Chrnb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrnb4
Entrez Gene ID	25103
Full Name	cholinergic receptor nicotinic beta 4 subunit
General protein information	Preferred Names cholinergic receptor nicotinic beta 4 subunit Names neuronal acetylcholine receptor subunit beta-4 Acetylcholine receptor beta 4 N-alpha 2 cholinergic receptor, nicotinic beta 4 cholinergic receptor, nicotinic, beta 4 (neuronal) cholinergic receptor, nicotinic, beta polypeptide 4 neuronal acetylcholine receptor non-alpha-2 chain nicotinic acetylcholine receptor-related protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	may act as a subunit of a neuronal nicotinic acetylcholine receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_052806.2	NP_434693.1	neuronal acetylcholine receptor subunit beta-4 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CHRNB4	NP_990150.1
Homo sapiens (human)	CHRNB4	NP_000741.1
Bos taurus (cattle)	CHRNB4	NP_776942.1
Xenopus tropicalis (tropical clawed frog)	chrnb4	XP_002934622.2
Pan troglodytes (chimpanzee)	CHRNB4	XP_510529.3
Canis lupus familiaris (dog)	CHRNB4	XP_852548.2
Rattus norvegicus (Norway rat)	Chrnb4	NP_434693.1
Danio rerio (zebrafish)	LOC568566	XP_696993.2
	LOC716649	XP_001107489.2
Mus musculus (house mouse)	Chrnb4	NP_683746.1

Gene Ontology
Bibliography

Related articles in PubMed

Inflammation-induced increase in nicotinic acetylcholine receptor current in cutaneous nociceptive DRG neurons from the adult rat.
Zhang XL, Albers KM, Gold MS
Neuroscience284483-99(2015 Jan)

Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: relevance to neuropsychiatric disorders.
Santoro ML, Santos CM, Ota VK, Gadelha A, Stilhano RS, Diana MC, Silva PN, Sp?ndola LM, Melaragno MI, Bressan RA, Han SW, Ab?lio VC, Belangero SI
Psychiatry research219(3)674-9(2014 Nov)

Subunit composition and kinetics of the Renshaw cell heteromeric nicotinic receptors.
Lamotte d'Incamps B, Ascher P
Biochemical pharmacology86(8)1114-21(2013 Oct)

Nicotinic acetylcholine receptors in dorsal root ganglion neurons include the ?6?4* subtype.
Hone AJ, Meyer EL, McIntyre M, McIntosh JM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(2)917-26(2012 Feb)

Quantitative analysis of the heteromeric neuronal nicotinic receptors in the rat hippocampus.
Lomazzo E, MacArthur L, Yasuda RP, Wolfe BB, Kellar KJ
Journal of neurochemistry115(3)625-34(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest the fast nAChR current in cutaneous dorsal root ganglia neurons was carried by homomeric alpha7 nAChRs while the slow current was carried by heteromeric (most likely alpha3beta4) nAChRs
Title: Inflammation-induced increase in nicotinic acetylcholine receptor current in cutaneous nociceptive DRG neurons from the adult rat.

Four genes were significantly downregulated in the PFC [prefrontal cortex ]of SHR[spontaneously hypertensive rat ] compared to Wistar rats (Gad2, Chrnb4, Slc5a7, and Qrfpr) and none in nucleus accumbens.
Title: Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: relevance to neuropsychiatric disorders.

alpha6beta4* nAChRs are among the subtypes expressed by dorsal root ganglion neurons, and to our knowledge, this is the first demonstration of alpha6beta4* in neurons outside the CNS
Title: Nicotinic acetylcholine receptors in dorsal root ganglion neurons include the ?6?4* subtype.

Loop 9 has a different role in the function of homomeric and heteromeric receptors.
Title: Role of loop 9 on the function of neuronal nicotinic receptors.

The role of negatively charged amino acids in the F-loop of the beta 4 subunit in channel activation and desensitization was studied using the patch-clamp technique.
Title: Role of negatively charged amino acids in beta 4 F-loop in activation and desensitization of alpha 3 beta 4 rat neuronal nicotinic receptors.

The following Chrnb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrnb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09161	NM_052806.2 Latest version!	Rattus norvegicus cholinergic receptor nicotinic beta 4 subunit (Chrnb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrnb4 ( NM_052806.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_434693.1 Rattus norvegicus cholinergic receptor nicotinic beta 4 subunit (Chrnb4), mRNA.

Price & Availability↑

CloneID	ORa09161
Clone ID Related Accession (Same CDS sequence)	NM_052806.2
Accession Version	NM_052806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal acetylcholine receptor subunit beta-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY574260.1. On Jun 10, 2007 this sequence version replaced NM_052806.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY574260.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632252, SAMN00632259 [ECO:0000348] ##Evidence-Data-END##

ATGAGGGGTA CGCCCCTGCT CCTCGTCTCT CTGTTCTCTC TGCTTCAGGA CGGGGACTGC 
CGCCTGGCCA ACGCAGAGGA GAAGCTGATG GATGACCTCC TGAACAAAAC CCGGTACAAC 
AACCTGATCC GCCCAGCCAC CAGCTCCTCT CAGCTCATCT CCATCCGCCT GGAGCTATCA 
CTGTCCCAGC TCATCAGTGT GAATGAGCGA GAACAGATCA TGACCACCAG CATCTGGCTG 
AAACAGGAAT GGACTGACTA CCGCCTGGCC TGGAACAGCT CCTGCTATGA AGGGGTGAAC 
ATTCTGAGGA TCCCCGCAAA GCGTGTCTGG TTGCCTGACA TCGTGTTGTA CAACAATGCC 
GATGGCACCT ATGAGGTGTC TGTCTACACC AACGTGATTG TGCGTTCCAA TGGCAGCATC 
CAGTGGCTGC CCCCTGCTAT CTACAAGAGT GCCTGCAAGA TTGAGGTGAA GCACTTTCCC 
TTCGACCAGC AGAACTGCAC CCTCAAATTC CGCTCCTGGA CCTATGACCA CACGGAGATT 
GACATGGTTC TTAAGTCGCC CACGGCCATC ATGGATGACT TCACCCCCAG TGGTGAATGG 
GACATTGTGG CCCTCCCAGG ACGGAGGACG GTGAACCCTC AGGACCCCAG CTACGTGGAC 
GTGACCTATG ACTTCATCAT CAAGCGCAAG CCGCTCTTCT ACACCATCAA TCTTATCATT 
CCTTGTGTGC TCATCACCTC GCTGGCTATC CTGGTCTTCT ACCTGCCCTC CGACTGTGGG 
GAGAAGATGA CGCTCTGCAT CTCTGTGCTG CTGGCACTCA CGTTCTTCCT GCTGCTCATC 
TCCAAGATCG TGCCTCCCAC CTCCCTTGAC ATACCGCTCA TTGGCAAGTA CCTCTTGTTC 
ACCATGGTGC TGGTCACCTT TTCCATCGTC ACCACTGTGT GTGTCCTCAA TGTGCACCAC 
CGCTCACCCA GCACTCACAC CATGGCATCC TGGGTCAAGG AGTGCTTCCT GCACAAACTG 
CCCACCTTCC TCTTCATGAA GCGTCCCGGT CTTGAAGTCA GCCTGGTCAG GGTCCCTCAT 
CCCAGCCAGC TGCACTTGGC CACAGCTGAT ACTGCAGCCA CCTCTGCCTT AGGCCCCACC 
AGCCCATCCA ACCTCTATGG GAGTTCCATG TACTTTGTGA ACCCTGTCCC TGCCGCTCCT 
AAGTCTGCAG TCAGCTCCCA CACAGCAGGC CTCCCCAGGG ATGCCCGTCT GAGGTCCTCC 
GGGAGGTTCC GGGAAGATCT ACAGGAAGCA TTAGAGGGTG TCAGCTTCAT CGCCCAGCAT 
CTGGAGAGCG ATGACCGAGA TCAAAGTGTC ATCGAGGACT GGAAGTTCGT CGCGATGGTT 
GTTGACCGCC TGTTCCTGTG GGTGTTCGTG TTTGTGTGTA TTCTGGGCAC CATGGGGCTC 
TTCCTGCCAC CCCTTTTCCA GATCCACGCA CCCTCCAAGG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_434693.1
CDS	1..1488
Translation	MRGTPLLLVSLFSLLQDGDCRLANAEEKLMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVSLVRVPHPSQLHLATADTAATSALGPTSPSNLYGSSMYFVNPVPAAPKSAVSSHTAGLPRDARLRSSGRFREDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVFVCILGTMGLFLPPLFQIHAPSKDS

Target ORF information:

RefSeq Version	NM_052806.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic beta 4 subunit (Chrnb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052806.2

ORF Insert Sequence:

ATGAGGGGTA CGCCCCTGCT CCTCGTCTCT CTGTTCTCTC TGCTTCAGGA CGGGGACTGC 
CGCCTGGCCA ACGCAGAGGA GAAGCTGATG GATGACCTCC TGAACAAAAC CCGGTACAAC 
AACCTGATCC GCCCAGCCAC CAGCTCCTCT CAGCTCATCT CCATCCGCCT GGAGCTATCA 
CTGTCCCAGC TCATCAGTGT GAATGAGCGA GAACAGATCA TGACCACCAG CATCTGGCTG 
AAACAGGAAT GGACTGACTA CCGCCTGGCC TGGAACAGCT CCTGCTATGA AGGGGTGAAC 
ATTCTGAGGA TCCCCGCAAA GCGTGTCTGG TTGCCTGACA TCGTGTTGTA CAACAATGCC 
GATGGCACCT ATGAGGTGTC TGTCTACACC AACGTGATTG TGCGTTCCAA TGGCAGCATC 
CAGTGGCTGC CCCCTGCTAT CTACAAGAGT GCCTGCAAGA TTGAGGTGAA GCACTTTCCC 
TTCGACCAGC AGAACTGCAC CCTCAAATTC CGCTCCTGGA CCTATGACCA CACGGAGATT 
GACATGGTTC TTAAGTCGCC CACGGCCATC ATGGATGACT TCACCCCCAG TGGTGAATGG 
GACATTGTGG CCCTCCCAGG ACGGAGGACG GTGAACCCTC AGGACCCCAG CTACGTGGAC 
GTGACCTATG ACTTCATCAT CAAGCGCAAG CCGCTCTTCT ACACCATCAA TCTTATCATT 
CCTTGTGTGC TCATCACCTC GCTGGCTATC CTGGTCTTCT ACCTGCCCTC CGACTGTGGG 
GAGAAGATGA CGCTCTGCAT CTCTGTGCTG CTGGCACTCA CGTTCTTCCT GCTGCTCATC 
TCCAAGATCG TGCCTCCCAC CTCCCTTGAC ATACCGCTCA TTGGCAAGTA CCTCTTGTTC 
ACCATGGTGC TGGTCACCTT TTCCATCGTC ACCACTGTGT GTGTCCTCAA TGTGCACCAC 
CGCTCACCCA GCACTCACAC CATGGCATCC TGGGTCAAGG AGTGCTTCCT GCACAAACTG 
CCCACCTTCC TCTTCATGAA GCGTCCCGGT CTTGAAGTCA GCCTGGTCAG GGTCCCTCAT 
CCCAGCCAGC TGCACTTGGC CACAGCTGAT ACTGCAGCCA CCTCTGCCTT AGGCCCCACC 
AGCCCATCCA ACCTCTATGG GAGTTCCATG TACTTTGTGA ACCCTGTCCC TGCCGCTCCT 
AAGTCTGCAG TCAGCTCCCA CACAGCAGGC CTCCCCAGGG ATGCCCGTCT GAGGTCCTCC 
GGGAGGTTCC GGGAAGATCT ACAGGAAGCA TTAGAGGGTG TCAGCTTCAT CGCCCAGCAT 
CTGGAGAGCG ATGACCGAGA TCAAAGTGTC ATCGAGGACT GGAAGTTCGT CGCGATGGTT 
GTTGACCGCC TGTTCCTGTG GGTGTTCGTG TTTGTGTGTA TTCTGGGCAC CATGGGGCTC 
TTCCTGCCAC CCCTTTTCCA GATCCACGCA CCCTCCAAGG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inflammation-induced increase in nicotinic acetylcholine receptor current in cutaneous nociceptive DRG neurons from the adult rat.
Neuroscience284483-99(2015 Jan)
Zhang XL,Albers KM,Gold MS

Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: relevance to neuropsychiatric disorders.
Psychiatry research219(3)674-9(2014 Nov)
Santoro ML,Santos CM,Ota VK,Gadelha A,Stilhano RS,Diana MC,Silva PN,Sp?ndola LM,Melaragno MI,Bressan RA,Han SW,Ab?lio VC,Belangero SI

Subunit composition and kinetics of the Renshaw cell heteromeric nicotinic receptors.
Biochemical pharmacology86(8)1114-21(2013 Oct)
Lamotte d'Incamps B,Ascher P

Nicotinic acetylcholine receptors in dorsal root ganglion neurons include the ?6?4* subtype.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(2)917-26(2012 Feb)
Hone AJ,Meyer EL,McIntyre M,McIntosh JM

Quantitative analysis of the heteromeric neuronal nicotinic receptors in the rat hippocampus.
Journal of neurochemistry115(3)625-34(2010 Nov)
Lomazzo E,MacArthur L,Yasuda RP,Wolfe BB,Kellar KJ

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Chrnb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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