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PRICKLE1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PRICKLE1
Entrez Gene ID 144165
Full Name prickle planar cell polarity protein 1
Synonyms EPM1B,RILP
General protein information
Preferred Names

prickle planar cell polarity protein 1

Names

prickle-like protein 1
REST (RE-1 silencing transcription factor)/NRSF (neuron-restrictive silencer factor)-interacting LIM domain protein
REST/NRSF interacting LIM domain protein
REST/NRSF-interacting LIM domain protein 1
prickle homolog 1
prickle-like 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

12

12q12

Summary This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009].
Disorder MIM:

608500

Disorder Html: Epilepsy, progressive myoclonic 1B, 612437 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001144883.1 NP_001138355.1 prickle-like protein 1
XM_017018839.1 XP_016874328.1 prickle-like protein 1 isoform X1
XM_017018838.1 XP_016874327.1 prickle-like protein 1 isoform X1
XM_017018840.1 XP_016874329.1 prickle-like protein 1 isoform X1
XM_011537946.1 XP_011536248.1 prickle-like protein 1 isoform X1
XM_011537947.2 XP_011536249.1 prickle-like protein 1 isoform X1
NM_001144881.1 NP_001138353.1 prickle-like protein 1
NM_001144882.1 NP_001138354.1 prickle-like protein 1
NM_153026.2 NP_694571.2 prickle-like protein 1



Bos taurus (cattle) PRICKLE1 NP_001096004.1
Mus musculus (house mouse) Prickle1 NP_001028389.1
Rattus norvegicus (Norway rat) Prickle1 NP_955428.1
Xenopus tropicalis (tropical clawed frog) prickle1 NP_001016939.1
Pan troglodytes (chimpanzee) PRICKLE1 XP_001166831.1
Gallus gallus (chicken) PRICKLE1 XP_416036.2
Homo sapiens (human) PRICKLE1 NP_001138355.1
Canis lupus familiaris (dog) PRICKLE1 XP_005637023.1
Danio rerio (zebrafish) prickle1a NP_899185.2
Macaca mulatta (rhesus monkey) PRICKLE1 XP_002798581.1


Related articles in PubMed

A proteome-scale map of the human interactome network.
Rolland T, Taşan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabási AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP, Vidal M
Cell159(5)1212-1226(2014 Nov)

PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders.
Paemka L, Mahajan VB, Skeie JM, Sowers LP, Ehaideb SN, Gonzalez-Alegre P, Sasaoka T, Tao H, Miyagi A, Ueno N, Takao K, Miyakawa T, Wu S, Darbro BW, Ferguson PJ, Pieper AA, Britt JK, Wemmie JA, Rudd DS, Wassink T, El-Shanti H, Mefford HC, Carvill GL, Manak JR, Bassuk AG
PloS one8(12)e80737(2013)

Mink1 regulates β-catenin-independent Wnt signaling via Prickle phosphorylation.
Daulat AM, Luu O, Sing A, Zhang L, Wrana JL, McNeill H, Winklbauer R, Angers S
Molecular and cellular biology32(1)173-85(2012 Jan)

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
Fox CS, Liu Y, White CC, Feitosa M, Smith AV, Heard-Costa N, Lohman K, , , , Johnson AD, Foster MC, Greenawalt DM, Griffin P, Ding J, Newman AB, Tylavsky F, Miljkovic I, Kritchevsky SB, Launer L, Garcia M, Eiriksdottir G, Carr JJ, Gudnason V, Harris TB, Cupples LA, Borecki IB
PLoS genetics8(5)e1002695(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prickle1 localized to the membrane through its farnesyl moiety, and the membrane localization was necessary for Prickle1 to regulate migration, to bind to CLASPs and LL5beta, and to promote microtubule targeting of focal adhesions.
Title: Prickle1 promotes focal adhesion disassembly in cooperation with the CLASP-LL5β complex in migrating cells.

upregulation of PRICKLE1 in basal breast cancers, a subtype characterized by high metastatic potential, is associated with poor metastasis-free survival.
Title: PRICKLE1 Contributes to Cancer Cell Dissemination through Its Interaction with mTORC2.

Our experimental data demonstrate that high expression of Prickle1 and Vangl2 reduce the growth of neuroblastoma cells and indicate different roles of PCP proteins in tumorigenic cells compared to normal cells.
Title: Planar cell polarity gene expression correlates with tumor cell viability and prognostic outcome in neuroblastoma.

these findings suggest PRICKLE1 mutations contribute to ASD by disrupting the interaction with SYN1 and regulation of synaptic vesicles.
Title: PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders.

MINK1 interacts with and phosphorylates PRICKLE1 and PRICKLE2.
Title: Mink1 regulates β-catenin-independent Wnt signaling via Prickle phosphorylation.

The following PRICKLE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRICKLE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22315 NM_001144882.1
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 NM_001144881.1
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 NM_153026.2
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 NM_001144883.1
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 XM_017018840.1
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 XM_011537946.1
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 XM_017018839.1
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 XM_011537947.2
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 XM_017018838.1
Latest version!
Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version NM_001144883.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-01-17
Organism Homo sapiens(Human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA321918.1, AK122881.1, CN390789.1, AK056189.1, AC079601.25 and BE858932.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122881.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138355.1
CDS284..2779
Misc Feature(1)257..259
Misc Feature(2)347..637
Misc Feature(3)659..835
Misc Feature(4)order(659..661,668..670,737..739,746..748,755..757,764..766,818..820,827..829)
Misc Feature(5)848..1015
Misc Feature(6)order(854..856,863..865,917..919,926..928,935..937,944..946,998..1000,1007..1009)
Misc Feature(7)1028..1204
Misc Feature(8)order(1034..1036,1043..1045,1100..1102,1115..1117,1124..1126,1133..1135,1187..1189,1199..1201)
Misc Feature(9)1226..1228
Misc Feature(10)2054..2056
Misc Feature(11)2063..2065
Misc Feature(12)2330..2332
Misc Feature(13)2765..2767
Exon (1)1..238
Exon (2)239..415
Exon (3)416..529
Exon (4)530..667
Exon (5)668..871
Exon (6)872..1058
Exon (7)1059..1922
Exon (8)1923..4250
Translation

Target ORF information:

RefSeq Version NM_001144883.1
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001144883.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version NM_001144881.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-01-17
Organism Homo sapiens(Human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122877.1, AC079601.25, BC114939.1 and BE858932.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138353.1
CDS288..2783
Misc Feature(1)261..263
Misc Feature(2)351..641
Misc Feature(3)663..839
Misc Feature(4)order(663..665,672..674,741..743,750..752,759..761,768..770,822..824,831..833)
Misc Feature(5)852..1019
Misc Feature(6)order(858..860,867..869,921..923,930..932,939..941,948..950,1002..1004,1011..1013)
Misc Feature(7)1032..1208
Misc Feature(8)order(1038..1040,1047..1049,1104..1106,1119..1121,1128..1130,1137..1139,1191..1193,1203..1205)
Misc Feature(9)1230..1232
Misc Feature(10)2058..2060
Misc Feature(11)2067..2069
Misc Feature(12)2334..2336
Misc Feature(13)2769..2771
Exon (1)1..239
Exon (2)240..419
Exon (3)420..533
Exon (4)534..671
Exon (5)672..875
Exon (6)876..1062
Exon (7)1063..1926
Exon (8)1927..4254
Translation

Target ORF information:

RefSeq Version NM_001144881.1
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001144881.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version NM_001144882.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-01-17
Organism Homo sapiens(Human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA321918.1, BC114940.1, AC079601.25 and BE858932.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF399844.1, AK122881.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138354.1
CDS287..2782
Misc Feature(1)260..262
Misc Feature(2)350..640
Misc Feature(3)662..838
Misc Feature(4)order(662..664,671..673,740..742,749..751,758..760,767..769,821..823,830..832)
Misc Feature(5)851..1018
Misc Feature(6)order(857..859,866..868,920..922,929..931,938..940,947..949,1001..1003,1010..1012)
Misc Feature(7)1031..1207
Misc Feature(8)order(1037..1039,1046..1048,1103..1105,1118..1120,1127..1129,1136..1138,1190..1192,1202..1204)
Misc Feature(9)1229..1231
Misc Feature(10)2057..2059
Misc Feature(11)2066..2068
Misc Feature(12)2333..2335
Misc Feature(13)2768..2770
Exon (1)1..238
Exon (2)239..418
Exon (3)419..532
Exon (4)533..670
Exon (5)671..874
Exon (6)875..1061
Exon (7)1062..1925
Exon (8)1926..4253
Translation

Target ORF information:

RefSeq Version NM_001144882.1
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001144882.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version NM_153026.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-01-17
Organism Homo sapiens(Human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA413584.1, BC114939.1, AC079601.25 and BE858932.1. This sequence is a reference standard in the RefSeqGene project. On or before Jan 30, 2009 this sequence version replaced XM_001718043.1, XM_001714454.1, NM_153026.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF399844.1, AK056499.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_694571.2
CDS355..2850
Misc Feature(1)328..330
Misc Feature(2)418..708
Misc Feature(3)730..906
Misc Feature(4)order(730..732,739..741,808..810,817..819,826..828,835..837,889..891,898..900)
Misc Feature(5)919..1086
Misc Feature(6)order(925..927,934..936,988..990,997..999,1006..1008,1015..1017,1069..1071,1078..1080)
Misc Feature(7)1099..1275
Misc Feature(8)order(1105..1107,1114..1116,1171..1173,1186..1188,1195..1197,1204..1206,1258..1260,1270..1272)
Misc Feature(9)1297..1299
Misc Feature(10)2125..2127
Misc Feature(11)2134..2136
Misc Feature(12)2401..2403
Misc Feature(13)2836..2838
Exon (1)1..306
Exon (2)307..486
Exon (3)487..600
Exon (4)601..738
Exon (5)739..942
Exon (6)943..1129
Exon (7)1130..1993
Exon (8)1994..4321
Translation

Target ORF information:

RefSeq Version NM_153026.2
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153026.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version XM_017018839.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product prickle-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016874328.1
CDS351..2846
Misc Feature(1)414..704
Misc Feature(2)726..902
Misc Feature(3)order(726..728,735..737,804..806,813..815,822..824,831..833,885..887,894..896)
Misc Feature(4)915..1082
Misc Feature(5)order(921..923,930..932,984..986,993..995,1002..1004,1011..1013,1065..1067,1074..1076)
Misc Feature(6)1095..1271
Misc Feature(7)order(1101..1103,1110..1112,1167..1169,1182..1184,1191..1193,1200..1202,1254..1256,1266..1268)
Translation

Target ORF information:

RefSeq Version XM_017018839.1
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017018839.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version XM_017018838.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product prickle-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016874327.1
CDS151..2646
Misc Feature(1)214..504
Misc Feature(2)526..702
Misc Feature(3)order(526..528,535..537,604..606,613..615,622..624,631..633,685..687,694..696)
Misc Feature(4)715..882
Misc Feature(5)order(721..723,730..732,784..786,793..795,802..804,811..813,865..867,874..876)
Misc Feature(6)895..1071
Misc Feature(7)order(901..903,910..912,967..969,982..984,991..993,1000..1002,1054..1056,1066..1068)
Translation

Target ORF information:

RefSeq Version XM_017018838.1
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017018838.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version XM_017018840.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product prickle-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016874329.1
CDS171..2666
Misc Feature(1)234..524
Misc Feature(2)546..722
Misc Feature(3)order(546..548,555..557,624..626,633..635,642..644,651..653,705..707,714..716)
Misc Feature(4)735..902
Misc Feature(5)order(741..743,750..752,804..806,813..815,822..824,831..833,885..887,894..896)
Misc Feature(6)915..1091
Misc Feature(7)order(921..923,930..932,987..989,1002..1004,1011..1013,1020..1022,1074..1076,1086..1088)
Translation

Target ORF information:

RefSeq Version XM_017018840.1
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017018840.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version XM_011537946.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product prickle-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536248.1
CDS106..2601
Misc Feature(1)169..459
Misc Feature(2)481..657
Misc Feature(3)order(481..483,490..492,559..561,568..570,577..579,586..588,640..642,649..651)
Misc Feature(4)670..837
Misc Feature(5)order(676..678,685..687,739..741,748..750,757..759,766..768,820..822,829..831)
Misc Feature(6)850..1026
Misc Feature(7)order(856..858,865..867,922..924,937..939,946..948,955..957,1009..1011,1021..1023)
Translation

Target ORF information:

RefSeq Version XM_011537946.1
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537946.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144883.1 , XM_017018839.1 , XM_017018838.1 , XM_017018840.1 , XM_011537946.1 , XM_011537947.2 , NM_001144881.1 , NM_001144882.1 , NM_153026.2
Accession Version XM_011537947.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product prickle-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536249.1
CDS337..2832
Misc Feature(1)400..690
Misc Feature(2)712..888
Misc Feature(3)order(712..714,721..723,790..792,799..801,808..810,817..819,871..873,880..882)
Misc Feature(4)901..1068
Misc Feature(5)order(907..909,916..918,970..972,979..981,988..990,997..999,1051..1053,1060..1062)
Misc Feature(6)1081..1257
Misc Feature(7)order(1087..1089,1096..1098,1153..1155,1168..1170,1177..1179,1186..1188,1240..1242,1252..1254)
Translation

Target ORF information:

RefSeq Version XM_011537947.2
Organism Homo sapiens(Human)
Definition Homo sapiens prickle planar cell polarity protein 1 (PRICKLE1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537947.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A proteome-scale map of the human interactome network.
Cell159(5)1212-1226(2014 Nov)
Rolland T,Taşan M,Charloteaux B,Pevzner SJ,Zhong Q,Sahni N,Yi S,Lemmens I,Fontanillo C,Mosca R,Kamburov A,Ghiassian SD,Yang X,Ghamsari L,Balcha D,Begg BE,Braun P,Brehme M,Broly MP,Carvunis AR,Convery-Zupan D,Corominas R,Coulombe-Huntington J,Dann E,Dreze M,Dricot A,Fan C,Franzosa E,Gebreab F,Gutierrez BJ,Hardy MF,Jin M,Kang S,Kiros R,Lin GN,Luck K,MacWilliams A,Menche J,Murray RR,Palagi A,Poulin MM,Rambout X,Rasla J,Reichert P,Romero V,Ruyssinck E,Sahalie JM,Scholz A,Shah AA,Sharma A,Shen Y,Spirohn K,Tam S,Tejeda AO,Trigg SA,Twizere JC,Vega K,Walsh J,Cusick ME,Xia Y,Barabási AL,Iakoucheva LM,Aloy P,De Las Rivas J,Tavernier J,Calderwood MA,Hill DE,Hao T,Roth FP,Vidal M


book

PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders.
PloS one8(12)e80737(2013)
Paemka L,Mahajan VB,Skeie JM,Sowers LP,Ehaideb SN,Gonzalez-Alegre P,Sasaoka T,Tao H,Miyagi A,Ueno N,Takao K,Miyakawa T,Wu S,Darbro BW,Ferguson PJ,Pieper AA,Britt JK,Wemmie JA,Rudd DS,Wassink T,El-Shanti H,Mefford HC,Carvill GL,Manak JR,Bassuk AG


book

Mink1 regulates β-catenin-independent Wnt signaling via Prickle phosphorylation.
Molecular and cellular biology32(1)173-85(2012 Jan)
Daulat AM,Luu O,Sing A,Zhang L,Wrana JL,McNeill H,Winklbauer R,Angers S


book

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
PLoS genetics8(5)e1002695(2012)
Fox CS,Liu Y,White CC,Feitosa M,Smith AV,Heard-Costa N,Lohman K,,,,Johnson AD,Foster MC,Greenawalt DM,Griffin P,Ding J,Newman AB,Tylavsky F,Miljkovic I,Kritchevsky SB,Launer L,Garcia M,Eiriksdottir G,Carr JJ,Gudnason V,Harris TB,Cupples LA,Borecki IB


 
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