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GBA cDNA ORF clone, Homo sapiens (human)

Gene Symbol GBA
Entrez Gene ID 2629
Full Name glucosidase, beta, acid
Synonyms GBA1, GCB, GLUC
General protein information
Preferred Names
glucosylceramidase
Names
glucosylceramidase
alglucerase
imiglucerase
acid beta-glucosidase
beta-glucocerebrosidase
lysosomal glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
NP_000148.2
NP_001005741.1
NP_001005742.1
NP_001165282.1
NP_001165283.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21

Summary This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]. lac of sum
Disorder MIM:

606463

Disorder Html: Gaucher disease, type I, 230800(3); Gaucher disease, type II, 230900

mRNA and Protein(s)

mRNA Protein Name
XM_006711270 XP_006711333 glucosylceramidase isoform X1
XM_011509407 XP_011507709 glucosylceramidase isoform X1
XM_006726211 XP_006726274 glucosylceramidase isoform X1
XM_011546930 XP_011545232 glucosylceramidase isoform X1
NM_001005742 NP_001005742 glucosylceramidase isoform 1 precursor
NM_001005741 NP_001005741 glucosylceramidase isoform 1 precursor
NM_000157 NP_000148 glucosylceramidase isoform 1 precursor
NM_001171811 NP_001165282 glucosylceramidase isoform 2
NM_001171812 NP_001165283 glucosylceramidase isoform 3 precursor



Homo sapiens (human) GBA NP_001005742.1
Pan troglodytes (chimpanzee) GBA NP_001008997.1
Canis lupus familiaris (dog) GBA XP_855035.1
Bos taurus (cattle) GBA NP_001039886.1
Mus musculus (house mouse) Gba NP_001070879.1
Rattus norvegicus (Norway rat) Gba NP_001121111.1
Gallus gallus (chicken) LOC101748171 XP_004950417.1
Gallus gallus (chicken) LOC101750375 XP_004950434.1
Danio rerio (zebrafish) gba XP_687471.3
Caenorhabditis elegans gba-3 NP_503119.1
Caenorhabditis elegans gba-1 NP_001040750.1
Caenorhabditis elegans gba-2 NP_494208.2
Caenorhabditis elegans gba-4 NP_500785.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GBA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GBA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22331
XM_006711270 PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22331
XM_011509407 PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu54570 XM_006726211 PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu54570 XM_011546930 PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu22331
NM_001005742 Homo sapiens glucosidase, beta, acid (GBA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22331
NM_001005741 Homo sapiens glucosidase, beta, acid (GBA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22331
NM_000157 Homo sapiens glucosidase, beta, acid (GBA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22337 NM_001171811 Homo sapiens glucosidase, beta, acid (GBA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $265.30
$379.00
OHu22347 NM_001171812 Homo sapiens glucosidase, beta, acid (GBA), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $286.30
$409.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22331
Clone ID Related Accession (Same CDS sequence) NM_000157 , NM_001005741 , NM_001005742 , XM_006711270 , XM_011509407
Accession Version XM_006711270.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006711333.1
CDS330..1940
Misc Feature(1)447..1928(+)
Translation

Target ORF information:

RefSeq Version XM_006711270
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006711270

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22331
Clone ID Related Accession (Same CDS sequence) NM_000157 , NM_001005741 , NM_001005742 , XM_006711270 , XM_011509407
Accession Version XM_011509407.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011507709.1
CDS411..2021
Misc Feature(1)528..2009(+)
Translation

Target ORF information:

RefSeq Version XM_011509407
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509407

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54570
Clone ID Related Accession (Same CDS sequence) XM_006726211 , XM_011546930
Accession Version XM_006726211.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGT GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006726274.1
CDS330..1940
Misc Feature(1)447..1928(+)
Translation

Target ORF information:

RefSeq Version XM_006726211
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006726211

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGT GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54570
Clone ID Related Accession (Same CDS sequence) XM_006726211 , XM_011546930
Accession Version XM_011546930.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003315906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGT GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011545232.1
CDS411..2021
Misc Feature(1)528..2009(+)
Translation

Target ORF information:

RefSeq Version XM_011546930
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glucosidase, beta, acid (GBA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011546930

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGT GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22331
Clone ID Related Accession (Same CDS sequence) NM_000157 , NM_001005741 , NM_001005742 , XM_006711270 , XM_011509407
Accession Version NM_001005742.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300876.1 and AL713999.28. On Jan 28, 2010 this sequence version replaced gi:54607046. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK300876.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001005742.1
CDS407..2017
Misc Feature(1)116..118(+)
Misc Feature(2)524..2005(+)
Exon (1)1..357
Gene:GBA
Gene Synonym:
Exon (2)358..433
Gene:GBA
Gene Synonym:
Exon (3)434..521
Gene:GBA
Gene Synonym:
Exon (4)522..713
Gene:GBA
Gene Synonym:
Exon (5)714..860
Gene:GBA
Gene Synonym:
Exon (6)861..994
Gene:GBA
Gene Synonym:
Exon (7)995..1167
Gene:GBA
Gene Synonym:
Exon (8)1168..1405
Gene:GBA
Gene Synonym:
Exon (9)1406..1630
Gene:GBA
Gene Synonym:
Exon (10)1631..1794
Gene:GBA
Gene Synonym:
Exon (11)1795..1911
Gene:GBA
Gene Synonym:
Exon (12)1912..2564
Gene:GBA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001005742
Organism Homo sapiens (human)
Definition Homo sapiens glucosidase, beta, acid (GBA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001005742

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22331
Clone ID Related Accession (Same CDS sequence) NM_000157 , NM_001005741 , NM_001005742 , XM_006711270 , XM_011509407
Accession Version NM_001005741.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300876.1, AK291911.1 and AL713999.28. This sequence is a reference standard in the RefSeqGene project. On Jan 28, 2010 this sequence version replaced gi:54607044. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648487.1, AK291911.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001005741.1
CDS426..2036
Misc Feature(1)153..155(+)
Misc Feature(2)543..2024(+)
Exon (1)1..357
Gene:GBA
Gene Synonym:
Exon (2)358..452
Gene:GBA
Gene Synonym:
Exon (3)453..540
Gene:GBA
Gene Synonym:
Exon (4)541..732
Gene:GBA
Gene Synonym:
Exon (5)733..879
Gene:GBA
Gene Synonym:
Exon (6)880..1013
Gene:GBA
Gene Synonym:
Exon (7)1014..1186
Gene:GBA
Gene Synonym:
Exon (8)1187..1424
Gene:GBA
Gene Synonym:
Exon (9)1425..1649
Gene:GBA
Gene Synonym:
Exon (10)1650..1813
Gene:GBA
Gene Synonym:
Exon (11)1814..1930
Gene:GBA
Gene Synonym:
Exon (12)1931..2583
Gene:GBA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001005741
Organism Homo sapiens (human)
Definition Homo sapiens glucosidase, beta, acid (GBA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001005741

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22331
Clone ID Related Accession (Same CDS sequence) NM_000157 , NM_001005741 , NM_001005742 , XM_006711270 , XM_011509407
Accession Version NM_000157.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1611bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC297079.1, BC003356.1 and AL713999.28. On Jan 28, 2010 this sequence version replaced gi:54607042. Transcript Variant: This variant (1) encodes isoform 1. Variants 1, 2 and 3 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M19285.1, BC003356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000148.2
CDS167..1777
Misc Feature(1)65..67(+)
Misc Feature(2)284..1765(+)
Exon (1)1..193
Gene:GBA
Gene Synonym:
Exon (2)194..281
Gene:GBA
Gene Synonym:
Exon (3)282..473
Gene:GBA
Gene Synonym:
Exon (4)474..620
Gene:GBA
Gene Synonym:
Exon (5)621..754
Gene:GBA
Gene Synonym:
Exon (6)755..927
Gene:GBA
Gene Synonym:
Exon (7)928..1165
Gene:GBA
Gene Synonym:
Exon (8)1166..1390
Gene:GBA
Gene Synonym:
Exon (9)1391..1554
Gene:GBA
Gene Synonym:
Exon (10)1555..1671
Gene:GBA
Gene Synonym:
Exon (11)1672..2324
Gene:GBA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000157
Organism Homo sapiens (human)
Definition Homo sapiens glucosidase, beta, acid (GBA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000157

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGCC TGCTACTGAC CCTGCAGCCA GAACAGAAGT TCCAGAAAGT GAAGGGATTT
GGAGGGGCCA TGACAGATGC TGCTGCTCTC AACATCCTTG CCCTGTCACC CCCTGCCCAA
AATTTGCTAC TTAAATCGTA CTTCTCTGAA GAAGGAATCG GATATAACAT CATCCGGGTA
CCCATGGCCA GCTGTGACTT CTCCATCCGC ACCTACACCT ATGCAGACAC CCCTGATGAT
TTCCAGTTGC ACAACTTCAG CCTCCCAGAG GAAGATACCA AGCTCAAGAT ACCCCTGATT
CACCGAGCCC TGCAGTTGGC CCAGCGTCCC GTTTCACTCC TTGCCAGCCC CTGGACATCA
CCCACTTGGC TCAAGACCAA TGGAGCGGTG AATGGGAAGG GGTCACTCAA GGGACAGCCC
GGAGACATCT ACCACCAGAC CTGGGCCAGA TACTTTGTGA AGTTCCTGGA TGCCTATGCT
GAGCACAAGT TACAGTTCTG GGCAGTGACA GCTGAAAATG AGCCTTCTGC TGGGCTGTTG
AGTGGATACC CCTTCCAGTG CCTGGGCTTC ACCCCTGAAC ATCAGCGAGA CTTCATTGCC
CGTGACCTAG GTCCTACCCT CGCCAACAGT ACTCACCACA ATGTCCGCCT ACTCATGCTG
GATGACCAAC GCTTGCTGCT GCCCCACTGG GCAAAGGTGG TACTGACAGA CCCAGAAGCA
GCTAAATATG TTCATGGCAT TGCTGTACAT TGGTACCTGG ACTTTCTGGC TCCAGCCAAA
GCCACCCTAG GGGAGACACA CCGCCTGTTC CCCAACACCA TGCTCTTTGC CTCAGAGGCC
TGTGTGGGCT CCAAGTTCTG GGAGCAGAGT GTGCGGCTAG GCTCCTGGGA TCGAGGGATG
CAGTACAGCC ACAGCATCAT CACGAACCTC CTGTACCATG TGGTCGGCTG GACCGACTGG
AACCTTGCCC TGAACCCCGA AGGAGGACCC AATTGGGTGC GTAACTTTGT CGACAGTCCC
ATCATTGTAG ACATCACCAA GGACACGTTT TACAAACAGC CCATGTTCTA CCACCTTGGC
CACTTCAGCA AGTTCATTCC TGAGGGCTCC CAGAGAGTGG GGCTGGTTGC CAGTCAGAAG
AACGACCTGG ACGCAGTGGC ACTGATGCAT CCCGATGGCT CTGCTGTTGT GGTCGTGCTA
AACCGCTCCT CTAAGGATGT GCCTCTTACC ATCAAGGATC CTGCTGTGGG CTTCCTGGAG
ACAATCTCAC CTGGCTACTC CATTCACACC TACCTGTGGC GTCGCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22337
Clone ID Related Accession (Same CDS sequence) NM_001171811
Accession Version NM_001171811.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300876.1, AK300829.1 and AL713999.28. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300829.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGCTGA GTATGGGGCC CATCCAGGCT AATCACACGG GCACAGGCCT GCTACTGACC 
CTGCAGCCAG AACAGAAGTT CCAGAAAGTG AAGGGATTTG GAGGGGCCAT GACAGATGCT
GCTGCTCTCA ACATCCTTGC CCTGTCACCC CCTGCCCAAA ATTTGCTACT TAAATCGTAC
TTCTCTGAAG AAGGAATCGG ATATAACATC ATCCGGGTAC CCATGGCCAG CTGTGACTTC
TCCATCCGCA CCTACACCTA TGCAGACACC CCTGATGATT TCCAGTTGCA CAACTTCAGC
CTCCCAGAGG AAGATACCAA GCTCAAGATA CCCCTGATTC ACCGAGCCCT GCAGTTGGCC
CAGCGTCCCG TTTCACTCCT TGCCAGCCCC TGGACATCAC CCACTTGGCT CAAGACCAAT
GGAGCGGTGA ATGGGAAGGG GTCACTCAAG GGACAGCCCG GAGACATCTA CCACCAGACC
TGGGCCAGAT ACTTTGTGAA GTTCCTGGAT GCCTATGCTG AGCACAAGTT ACAGTTCTGG
GCAGTGACAG CTGAAAATGA GCCTTCTGCT GGGCTGTTGA GTGGATACCC CTTCCAGTGC
CTGGGCTTCA CCCCTGAACA TCAGCGAGAC TTCATTGCCC GTGACCTAGG TCCTACCCTC
GCCAACAGTA CTCACCACAA TGTCCGCCTA CTCATGCTGG ATGACCAACG CTTGCTGCTG
CCCCACTGGG CAAAGGTGGT ACTGACAGAC CCAGAAGCAG CTAAATATGT TCATGGCATT
GCTGTACATT GGTACCTGGA CTTTCTGGCT CCAGCCAAAG CCACCCTAGG GGAGACACAC
CGCCTGTTCC CCAACACCAT GCTCTTTGCC TCAGAGGCCT GTGTGGGCTC CAAGTTCTGG
GAGCAGAGTG TGCGGCTAGG CTCCTGGGAT CGAGGGATGC AGTACAGCCA CAGCATCATC
ACGAACCTCC TGTACCATGT GGTCGGCTGG ACCGACTGGA ACCTTGCCCT GAACCCCGAA
GGAGGACCCA ATTGGGTGCG TAACTTTGTC GACAGTCCCA TCATTGTAGA CATCACCAAG
GACACGTTTT ACAAACAGCC CATGTTCTAC CACCTTGGCC ACTTCAGCAA GTTCATTCCT
GAGGGCTCCC AGAGAGTGGG GCTGGTTGCC AGTCAGAAGA ACGACCTGGA CGCAGTGGCA
CTGATGCATC CCGATGGCTC TGCTGTTGTG GTCGTGCTAA ACCGCTCCTC TAAGGATGTG
CCTCTTACCA TCAAGGATCC TGCTGTGGGC TTCCTGGAGA CAATCTCACC TGGCTACTCC
ATTCACACCT ACCTGTGGCG TCGCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001165282.1
CDS504..1853
Misc Feature(1)153..155(+)
Misc Feature(2)504..1841(+)
Exon (1)1..357
Gene:GBA
Gene Synonym:
Exon (2)358..549
Gene:GBA
Gene Synonym:
Exon (3)550..696
Gene:GBA
Gene Synonym:
Exon (4)697..830
Gene:GBA
Gene Synonym:
Exon (5)831..1003
Gene:GBA
Gene Synonym:
Exon (6)1004..1241
Gene:GBA
Gene Synonym:
Exon (7)1242..1466
Gene:GBA
Gene Synonym:
Exon (8)1467..1630
Gene:GBA
Gene Synonym:
Exon (9)1631..1747
Gene:GBA
Gene Synonym:
Exon (10)1748..2400
Gene:GBA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001171811
Organism Homo sapiens (human)
Definition Homo sapiens glucosidase, beta, acid (GBA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001171811

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGCTGA GTATGGGGCC CATCCAGGCT AATCACACGG GCACAGGCCT GCTACTGACC 
CTGCAGCCAG AACAGAAGTT CCAGAAAGTG AAGGGATTTG GAGGGGCCAT GACAGATGCT
GCTGCTCTCA ACATCCTTGC CCTGTCACCC CCTGCCCAAA ATTTGCTACT TAAATCGTAC
TTCTCTGAAG AAGGAATCGG ATATAACATC ATCCGGGTAC CCATGGCCAG CTGTGACTTC
TCCATCCGCA CCTACACCTA TGCAGACACC CCTGATGATT TCCAGTTGCA CAACTTCAGC
CTCCCAGAGG AAGATACCAA GCTCAAGATA CCCCTGATTC ACCGAGCCCT GCAGTTGGCC
CAGCGTCCCG TTTCACTCCT TGCCAGCCCC TGGACATCAC CCACTTGGCT CAAGACCAAT
GGAGCGGTGA ATGGGAAGGG GTCACTCAAG GGACAGCCCG GAGACATCTA CCACCAGACC
TGGGCCAGAT ACTTTGTGAA GTTCCTGGAT GCCTATGCTG AGCACAAGTT ACAGTTCTGG
GCAGTGACAG CTGAAAATGA GCCTTCTGCT GGGCTGTTGA GTGGATACCC CTTCCAGTGC
CTGGGCTTCA CCCCTGAACA TCAGCGAGAC TTCATTGCCC GTGACCTAGG TCCTACCCTC
GCCAACAGTA CTCACCACAA TGTCCGCCTA CTCATGCTGG ATGACCAACG CTTGCTGCTG
CCCCACTGGG CAAAGGTGGT ACTGACAGAC CCAGAAGCAG CTAAATATGT TCATGGCATT
GCTGTACATT GGTACCTGGA CTTTCTGGCT CCAGCCAAAG CCACCCTAGG GGAGACACAC
CGCCTGTTCC CCAACACCAT GCTCTTTGCC TCAGAGGCCT GTGTGGGCTC CAAGTTCTGG
GAGCAGAGTG TGCGGCTAGG CTCCTGGGAT CGAGGGATGC AGTACAGCCA CAGCATCATC
ACGAACCTCC TGTACCATGT GGTCGGCTGG ACCGACTGGA ACCTTGCCCT GAACCCCGAA
GGAGGACCCA ATTGGGTGCG TAACTTTGTC GACAGTCCCA TCATTGTAGA CATCACCAAG
GACACGTTTT ACAAACAGCC CATGTTCTAC CACCTTGGCC ACTTCAGCAA GTTCATTCCT
GAGGGCTCCC AGAGAGTGGG GCTGGTTGCC AGTCAGAAGA ACGACCTGGA CGCAGTGGCA
CTGATGCATC CCGATGGCTC TGCTGTTGTG GTCGTGCTAA ACCGCTCCTC TAAGGATGTG
CCTCTTACCA TCAAGGATCC TGCTGTGGGC TTCCTGGAGA CAATCTCACC TGGCTACTCC
ATTCACACCT ACCTGTGGCG TCGCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22347
Clone ID Related Accession (Same CDS sequence) NM_001171812
Accession Version NM_001171812.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1464bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glucosylceramidase isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298900.1 and AL713999.28. Transcript Variant: This variant (5) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGAA TCGGATATAA CATCATCCGG GTACCCATGG CCAGCTGTGA CTTCTCCATC
CGCACCTACA CCTATGCAGA CACCCCTGAT GATTTCCAGT TGCACAACTT CAGCCTCCCA
GAGGAAGATA CCAAGCTCAA GATACCCCTG ATTCACCGAG CCCTGCAGTT GGCCCAGCGT
CCCGTTTCAC TCCTTGCCAG CCCCTGGACA TCACCCACTT GGCTCAAGAC CAATGGAGCG
GTGAATGGGA AGGGGTCACT CAAGGGACAG CCCGGAGACA TCTACCACCA GACCTGGGCC
AGATACTTTG TGAAGTTCCT GGATGCCTAT GCTGAGCACA AGTTACAGTT CTGGGCAGTG
ACAGCTGAAA ATGAGCCTTC TGCTGGGCTG TTGAGTGGAT ACCCCTTCCA GTGCCTGGGC
TTCACCCCTG AACATCAGCG AGACTTCATT GCCCGTGACC TAGGTCCTAC CCTCGCCAAC
AGTACTCACC ACAATGTCCG CCTACTCATG CTGGATGACC AACGCTTGCT GCTGCCCCAC
TGGGCAAAGG TGGTACTGAC AGACCCAGAA GCAGCTAAAT ATGTTCATGG CATTGCTGTA
CATTGGTACC TGGACTTTCT GGCTCCAGCC AAAGCCACCC TAGGGGAGAC ACACCGCCTG
TTCCCCAACA CCATGCTCTT TGCCTCAGAG GCCTGTGTGG GCTCCAAGTT CTGGGAGCAG
AGTGTGCGGC TAGGCTCCTG GGATCGAGGG ATGCAGTACA GCCACAGCAT CATCACGAAC
CTCCTGTACC ATGTGGTCGG CTGGACCGAC TGGAACCTTG CCCTGAACCC CGAAGGAGGA
CCCAATTGGG TGCGTAACTT TGTCGACAGT CCCATCATTG TAGACATCAC CAAGGACACG
TTTTACAAAC AGCCCATGTT CTACCACCTT GGCCACTTCA GCAAGTTCAT TCCTGAGGGC
TCCCAGAGAG TGGGGCTGGT TGCCAGTCAG AAGAACGACC TGGACGCAGT GGCACTGATG
CATCCCGATG GCTCTGCTGT TGTGGTCGTG CTAAACCGCT CCTCTAAGGA TGTGCCTCTT
ACCATCAAGG ATCCTGCTGT GGGCTTCCTG GAGACAATCT CACCTGGCTA CTCCATTCAC
ACCTACCTGT GGCGTCGCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001165283.1
CDS164..1627
Misc Feature(1)62..64(+)
Misc Feature(2)281..1615(+)
Exon (1)1..190
Gene:GBA
Gene Synonym:
Exon (2)191..278
Gene:GBA
Gene Synonym:
Exon (3)279..470
Gene:GBA
Gene Synonym:
Exon (4)471..604
Gene:GBA
Gene Synonym:
Exon (5)605..777
Gene:GBA
Gene Synonym:
Exon (6)778..1015
Gene:GBA
Gene Synonym:
Exon (7)1016..1240
Gene:GBA
Gene Synonym:
Exon (8)1241..1404
Gene:GBA
Gene Synonym:
Exon (9)1405..1521
Gene:GBA
Gene Synonym:
Exon (10)1522..2174
Gene:GBA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001171812
Organism Homo sapiens (human)
Definition Homo sapiens glucosidase, beta, acid (GBA), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001171812

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGTTTT CAAGTCCTTC CAGAGAGGAA TGTCCCAAGC CTTTGAGTAG GGTAAGCATC 
ATGGCTGGCA GCCTCACAGG ATTGCTTCTA CTTCAGGCAG TGTCGTGGGC ATCAGGTGCC
CGCCCCTGCA TCCCTAAAAG CTTCGGCTAC AGCTCGGTGG TGTGTGTCTG CAATGCCACA
TACTGTGACT CCTTTGACCC CCCGACCTTT CCTGCCCTTG GTACCTTCAG CCGCTATGAG
AGTACACGCA GTGGGCGACG GATGGAGCTG AGTATGGGGC CCATCCAGGC TAATCACACG
GGCACAGGAA TCGGATATAA CATCATCCGG GTACCCATGG CCAGCTGTGA CTTCTCCATC
CGCACCTACA CCTATGCAGA CACCCCTGAT GATTTCCAGT TGCACAACTT CAGCCTCCCA
GAGGAAGATA CCAAGCTCAA GATACCCCTG ATTCACCGAG CCCTGCAGTT GGCCCAGCGT
CCCGTTTCAC TCCTTGCCAG CCCCTGGACA TCACCCACTT GGCTCAAGAC CAATGGAGCG
GTGAATGGGA AGGGGTCACT CAAGGGACAG CCCGGAGACA TCTACCACCA GACCTGGGCC
AGATACTTTG TGAAGTTCCT GGATGCCTAT GCTGAGCACA AGTTACAGTT CTGGGCAGTG
ACAGCTGAAA ATGAGCCTTC TGCTGGGCTG TTGAGTGGAT ACCCCTTCCA GTGCCTGGGC
TTCACCCCTG AACATCAGCG AGACTTCATT GCCCGTGACC TAGGTCCTAC CCTCGCCAAC
AGTACTCACC ACAATGTCCG CCTACTCATG CTGGATGACC AACGCTTGCT GCTGCCCCAC
TGGGCAAAGG TGGTACTGAC AGACCCAGAA GCAGCTAAAT ATGTTCATGG CATTGCTGTA
CATTGGTACC TGGACTTTCT GGCTCCAGCC AAAGCCACCC TAGGGGAGAC ACACCGCCTG
TTCCCCAACA CCATGCTCTT TGCCTCAGAG GCCTGTGTGG GCTCCAAGTT CTGGGAGCAG
AGTGTGCGGC TAGGCTCCTG GGATCGAGGG ATGCAGTACA GCCACAGCAT CATCACGAAC
CTCCTGTACC ATGTGGTCGG CTGGACCGAC TGGAACCTTG CCCTGAACCC CGAAGGAGGA
CCCAATTGGG TGCGTAACTT TGTCGACAGT CCCATCATTG TAGACATCAC CAAGGACACG
TTTTACAAAC AGCCCATGTT CTACCACCTT GGCCACTTCA GCAAGTTCAT TCCTGAGGGC
TCCCAGAGAG TGGGGCTGGT TGCCAGTCAG AAGAACGACC TGGACGCAGT GGCACTGATG
CATCCCGATG GCTCTGCTGT TGTGGTCGTG CTAAACCGCT CCTCTAAGGA TGTGCCTCTT
ACCATCAAGG ATCCTGCTGT GGGCTTCCTG GAGACAATCT CACCTGGCTA CTCCATTCAC
ACCTACCTGT GGCGTCGCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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