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GJA1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol GJA1
Entrez Gene ID 2697
Full Name gap junction protein alpha 1
Synonyms AVSD3,CMDR,CX43,EKVP,EKVP3,GJAL,HLHS1,HSS,ODDD,PPKCA
General protein information
Preferred Names

gap junction protein alpha 1

Names

gap junction alpha-1 protein
connexin-43
gap junction 43 kDa heart protein
gap junction protein, alpha 1, 43kDa

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

6

6q22.31

Summary This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014].
Disorder MIM:

121014

Disorder Html: Oculodentodigital dysplasia, 164200 (3); Syndactyly, type III,

mRNA and Protein(s)

mRNA Protein Name
NM_000165.4 NP_000156.1 gap junction alpha-1 protein



Mus musculus (house mouse) Gja1 NP_034418.1
Xenopus tropicalis (tropical clawed frog) gja1 NP_988856.1
Macaca mulatta (rhesus monkey) GJA1 XP_001109617.1
Pan troglodytes (chimpanzee) GJA1 XP_001164338.1
Canis lupus familiaris (dog) GJA1 NP_001002951.2
Bos taurus (cattle) GJA1 NP_776493.1
Rattus norvegicus (Norway rat) Gja1 NP_036699.1
Homo sapiens (human) GJA1 NP_000156.1
Gallus gallus (chicken) GJA1 NP_989917.1
Danio rerio (zebrafish) cx43 NP_571113.1


Related articles in PubMed

Gap junction protein connexin43 deregulation contributes to bladder carcinogenesis via targeting MAPK pathway.
Ai XL, Chi Q, Qiu Y, Li HY, Li DJ, Wang JX, Wang ZY
Molecular and cellular biochemistry428(1-2)109-118(2017 Apr)

Cx43 Mediates Resistance against MPP⁺-Induced Apoptosis in SH-SY5Y Neuroblastoma Cells via Modulating the Mitochondrial Apoptosis Pathway.
Kim IS, Ganesan P, Choi DK
International journal of molecular sciences17(11)(2016 Nov)

Gap junction composed of connexin43 modulates 5‑fluorouracil, oxaliplatin and irinotecan resistance on colorectal cancers.
Zou ZW, Chen HJ, Yu JL, Huang ZH, Fang S, Lin XH
Molecular medicine reports14(5)4893-4900(2016 Nov)

CX43 Expression in Colonic Adenomas and Surrounding Mucosa Is a Marker of Malignant Potential.
Bišćanin A, Ljubičić N, Boban M, Baličević D, Pavić I, Bišćanin MM, Budimir I, Dorosulic Z, Duvnjak M
Anticancer research36(10)5437-5442(2016 10)

Role of CAPE on cardiomyocyte protection via connexin 43 regulation under hypoxia.
Chen CC, Kuo CY, Chen RF
International journal of medical sciences13(10)754-758(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cx45 mutant p.R75H is responsible for a novel disease entity of progressive atrial conduction system defects associated with craniofacial and dentodigital malformation.
Title: Progressive Atrial Conduction Defects Associated With Bone Malformation Caused by a Connexin-45 Mutation.

Connexin 43 might contribute to the stabilization of atheromatous plaque which is affected by oxidative stress.
Title: Protective effects of connexins in atheromatous plaques in patients of carotid artery stenosis.

resistance to 5FU, oxaliplatin and irinotecan in colon cancer cells was relative to Cx43 expression loss as cancer developed, which may indicate a novel basis for therapeutic strategy development to combat drug resistance in numerous cell types, in addition to colon cancer cells.
Title: Gap junction composed of connexin43 modulates 5‑fluorouracil, oxaliplatin and irinotecan resistance on colorectal cancers.

Connexin 43 expression is increased in human fetal membrane defects after fetoscopic surgery compared to controls, with preferential distribution in the fibroblast layer compared with the epithelial layer.
Title: Connexin 43 is overexpressed in human fetal membrane defects after fetoscopic surgery.

Caffeic acid phenethyl ester attenuates hypoxia-caused connexin 43 down-regulation and cardiomyocyte death.
Title: Role of CAPE on cardiomyocyte protection via connexin 43 regulation under hypoxia.

The following GJA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu24686 NM_000165.4
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Homo sapiens gap junction protein alpha 1 (GJA1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24686
Clone ID Related Accession (Same CDS sequence) NM_000165.4
Accession Version NM_000165.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product gap junction alpha-1 protein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP226987.1, BC026329.1, AL139098.15 and AW664595.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2014 this sequence version replaced NM_000165.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP226987.1, SRR1803614.274105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGGTGACT GGAGCGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA CTCAACTGCT 
GGAGGGAAGG TGTGGCTGTC AGTACTTTTC ATTTTCCGAA TCCTGCTGCT GGGGACAGCG
GTTGAGTCAG CCTGGGGAGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA GCAACCTGGT
TGTGAAAATG TCTGCTATGA CAAGTCTTTC CCAATCTCTC ATGTGCGCTT CTGGGTCCTG
CAGATCATAT TTGTGTCTGT ACCCACACTC TTGTACCTGG CTCATGTGTT CTATGTGATG
CGAAAGGAAG AGAAACTGAA CAAGAAAGAG GAAGAACTCA AGGTTGCCCA AACTGATGGT
GTCAATGTGG ACATGCACTT GAAGCAGATT GAGATAAAGA AGTTCAAGTA CGGTATTGAA
GAGCATGGTA AGGTGAAAAT GCGAGGGGGG TTGCTGCGAA CCTACATCAT CAGTATCCTC
TTCAAGTCTA TCTTTGAGGT GGCCTTCTTG CTGATCCAGT GGTACATCTA TGGATTCAGC
TTGAGTGCTG TTTACACTTG CAAAAGAGAT CCCTGCCCAC ATCAGGTGGA CTGTTTCCTC
TCTCGCCCCA CGGAGAAAAC CATCTTCATC ATCTTCATGC TGGTGGTGTC CTTGGTGTCC
CTGGCCTTGA ATATCATTGA ACTCTTCTAT GTTTTCTTCA AGGGCGTTAA GGATCGGGTT
AAGGGAAAGA GCGACCCTTA CCATGCGACC AGTGGTGCGC TGAGCCCTGC CAAAGACTGT
GGGTCTCAAA AATATGCTTA TTTCAATGGC TGCTCCTCAC CAACCGCTCC CCTCTCGCCT
ATGTCTCCTC CTGGGTACAA GCTGGTTACT GGCGACAGAA ACAATTCTTC TTGCCGCAAT
TACAACAAGC AAGCAAGTGA GCAAAACTGG GCTAATTACA GTGCAGAACA AAATCGAATG
GGGCAGGCGG GAAGCACCAT CTCTAACTCC CATGCACAGC CTTTTGATTT CCCCGATGAT
AACCAGAATT CTAAAAAACT AGCTGCTGGA CATGAATTAC AGCCACTAGC CATTGTGGAC
CAGCGACCTT CAAGCAGAGC CAGCAGTCGT GCCAGCAGCA GACCTCGGCC TGATGACCTG
GAGATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000156.1
CDS273..1421
Misc Feature(1)246..248
Misc Feature(2)279..971
Misc Feature(3)285..287
Misc Feature(4)312..380
Misc Feature(5)399..500
Misc Feature(6)501..569
Misc Feature(7)735..803
Misc Feature(8)765..965
Misc Feature(9)897..965
Misc Feature(10)1002..1418
Misc Feature(11)1011..1013
Misc Feature(12)1011..1013
Misc Feature(13)1035..1037
Misc Feature(14)1035..1037
Misc Feature(15)1035..1037
Misc Feature(16)1035..1037
Misc Feature(17)1056..1058
Misc Feature(18)1062..1418
Misc Feature(19)1065..1067
Misc Feature(20)1083..1085
Misc Feature(21)1095..1097
Misc Feature(22)1107..1109
Misc Feature(23)1116..1118
Misc Feature(24)1116..1118
Misc Feature(25)1116..1118
Misc Feature(26)1149..1208
Misc Feature(27)1188..1190
Misc Feature(28)1209..1211
Misc Feature(29)1212..1214
Misc Feature(30)1245..1247
Misc Feature(31)1245..1247
Misc Feature(32)1248..1250
Misc Feature(33)1254..1256
Misc Feature(34)1254..1256
Misc Feature(35)1260..1262
Misc Feature(36)1260..1262
Misc Feature(37)1302..1304
Misc Feature(38)1365..1367
Misc Feature(39)1365..1367
Misc Feature(40)1374..1376
Misc Feature(41)1374..1376
Misc Feature(42)1374..1376
Misc Feature(43)1374..1376
Misc Feature(44)1377..1379
Misc Feature(45)1377..1379
Misc Feature(46)1389..1391
Misc Feature(47)1389..1391
Exon (1)1..256
Exon (2)257..3169
Translation

Target ORF information:

RefSeq Version NM_000165.4
Organism Homo sapiens(Human)
Definition Homo sapiens gap junction protein alpha 1 (GJA1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000165.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGGTGACT GGAGCGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA CTCAACTGCT 
GGAGGGAAGG TGTGGCTGTC AGTACTTTTC ATTTTCCGAA TCCTGCTGCT GGGGACAGCG
GTTGAGTCAG CCTGGGGAGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA GCAACCTGGT
TGTGAAAATG TCTGCTATGA CAAGTCTTTC CCAATCTCTC ATGTGCGCTT CTGGGTCCTG
CAGATCATAT TTGTGTCTGT ACCCACACTC TTGTACCTGG CTCATGTGTT CTATGTGATG
CGAAAGGAAG AGAAACTGAA CAAGAAAGAG GAAGAACTCA AGGTTGCCCA AACTGATGGT
GTCAATGTGG ACATGCACTT GAAGCAGATT GAGATAAAGA AGTTCAAGTA CGGTATTGAA
GAGCATGGTA AGGTGAAAAT GCGAGGGGGG TTGCTGCGAA CCTACATCAT CAGTATCCTC
TTCAAGTCTA TCTTTGAGGT GGCCTTCTTG CTGATCCAGT GGTACATCTA TGGATTCAGC
TTGAGTGCTG TTTACACTTG CAAAAGAGAT CCCTGCCCAC ATCAGGTGGA CTGTTTCCTC
TCTCGCCCCA CGGAGAAAAC CATCTTCATC ATCTTCATGC TGGTGGTGTC CTTGGTGTCC
CTGGCCTTGA ATATCATTGA ACTCTTCTAT GTTTTCTTCA AGGGCGTTAA GGATCGGGTT
AAGGGAAAGA GCGACCCTTA CCATGCGACC AGTGGTGCGC TGAGCCCTGC CAAAGACTGT
GGGTCTCAAA AATATGCTTA TTTCAATGGC TGCTCCTCAC CAACCGCTCC CCTCTCGCCT
ATGTCTCCTC CTGGGTACAA GCTGGTTACT GGCGACAGAA ACAATTCTTC TTGCCGCAAT
TACAACAAGC AAGCAAGTGA GCAAAACTGG GCTAATTACA GTGCAGAACA AAATCGAATG
GGGCAGGCGG GAAGCACCAT CTCTAACTCC CATGCACAGC CTTTTGATTT CCCCGATGAT
AACCAGAATT CTAAAAAACT AGCTGCTGGA CATGAATTAC AGCCACTAGC CATTGTGGAC
CAGCGACCTT CAAGCAGAGC CAGCAGTCGT GCCAGCAGCA GACCTCGGCC TGATGACCTG
GAGATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Gap junction protein connexin43 deregulation contributes to bladder carcinogenesis via targeting MAPK pathway.
Molecular and cellular biochemistry428(1-2)109-118(2017 Apr)
Ai XL,Chi Q,Qiu Y,Li HY,Li DJ,Wang JX,Wang ZY


book

Cx43 Mediates Resistance against MPP⁺-Induced Apoptosis in SH-SY5Y Neuroblastoma Cells via Modulating the Mitochondrial Apoptosis Pathway.
International journal of molecular sciences17(11)(2016 Nov)
Kim IS,Ganesan P,Choi DK


book

Gap junction composed of connexin43 modulates 5‑fluorouracil, oxaliplatin and irinotecan resistance on colorectal cancers.
Molecular medicine reports14(5)4893-4900(2016 Nov)
Zou ZW,Chen HJ,Yu JL,Huang ZH,Fang S,Lin XH


book

CX43 Expression in Colonic Adenomas and Surrounding Mucosa Is a Marker of Malignant Potential.
Anticancer research36(10)5437-5442(2016 10)
Bišćanin A,Ljubičić N,Boban M,Baličević D,Pavić I,Bišćanin MM,Budimir I,Dorosulic Z,Duvnjak M


book

Role of CAPE on cardiomyocyte protection via connexin 43 regulation under hypoxia.
International journal of medical sciences13(10)754-758(2016)
Chen CC,Kuo CY,Chen RF


 
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