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HCLS1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol HCLS1
Entrez Gene ID 3059
Full Name hematopoietic cell-specific Lyn substrate 1
Synonyms CTTNL,HS1,lckBP1,p75
General protein information
Preferred Names

hematopoietic cell-specific Lyn substrate 1

Names

hematopoietic lineage cell-specific protein
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

3

3q13.33
Disorder MIM:

601306


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_005335.5 NP_005326.2 hematopoietic lineage cell-specific protein isoform 1
NM_001292041.1 NP_001278970.1 hematopoietic lineage cell-specific protein isoform 2
NM_001292041.2 NP_001278970.2 hematopoietic lineage cell-specific protein isoform 2
NM_005335.6 NP_005326.3 hematopoietic lineage cell-specific protein isoform 1

Pathways from BioSystems


Homologies


Homo sapiens (human) HCLS1 NP_005326.2
Mus musculus (house mouse) Hcls1 NP_032251.2
Rattus norvegicus (Norway rat) Hcls1 NP_001011898.1
Bos taurus (cattle) HCLS1 NP_001030229.1
Gallus gallus (chicken) HCLS1 NP_001026572.1
Pan troglodytes (chimpanzee) HCLS1 XP_516684.2
HCLS1 XP_001111514.1
Canis lupus familiaris (dog) HCLS1 XP_005639607.1

Gene Ontology


Bibliography

Related articles in PubMed

Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells.
Hasan MK, Yu J, Chen L, Cui B, Widhopf Ii GF, Rassenti L, Shen Z, Briggs SP, Kipps TJ
Leukemia31(12)2615-2622(2017 Dec)

Exome sequencing reveals recurrent germ line variants in patients with familial Waldenstr?m macroglobulinemia.
Roccaro AM, Sacco A, Shi J, Chiarini M, Perilla-Glen A, Manier S, Glavey S, Aljawai Y, Mishima Y, Kawano Y, Moschetta M, Correll M, Improgo MR, Brown JR, Imberti L, Rossi G, Castillo JJ, Treon SP, Freedman ML, Van Allen EM, Hide W, Hiller E, Rainville I, Ghobrial IM
Blood127(21)2598-606(2016 05)

SDF1?-induced interaction of the adapter proteins Nck and HS1 facilitates actin polymerization and migration in T?cells.
Lettau M, Kabelitz D, Janssen O
European journal of immunology45(2)551-61(2015 Feb)

Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors.
Habif G, Grasset MF, Kieffer-Jaquinod S, Kuhn L, Mouchiroud G, Gobert-Gosse S
Journal of proteomics78231-44(2013 Jan)

Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis.
Skokowa J, Klimiankou M, Klimenkova O, Lan D, Gupta K, Hussein K, Carrizosa E, Kusnetsova I, Li Z, Sustmann C, Ganser A, Zeidler C, Kreipe HH, Burkhardt J, Grosschedl R, Welte K
Nature medicine18(10)1550-9(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these studies indicate HS1 plays an important role in ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell migration.
Title: Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells.

SDF1alpha-induced interaction of the adapter proteins Nck and HS1 facilitates actin polymerization and migration in T cells.
Title: SDF1?-induced interaction of the adapter proteins Nck and HS1 facilitates actin polymerization and migration in T?cells.

Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors.
Title: Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors.

interaction of HCLS1 with LEF-1 is essential for G-CSF-triggered myeloid differentiation; data demonstrate the importance of HCLS1 in myelopoiesis in vitro and in vivo
Title: Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis.

HS1 overexpressed in leukemic as compared to normal B lymphocytes.
Title: HS1, a Lyn kinase substrate, is abnormally expressed in B-chronic lymphocytic leukemia and correlates with response to fludarabine-based regimen.

The following HCLS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCLS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24681 NM_005335.5
Latest version!		 Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu107365 NM_005335.6
Latest version!		 Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu108219 NM_001292041.2
Latest version!		 Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu24656 NM_001292041.1
Latest version!		 Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu24681
Clone ID Related Accession (Same CDS sequence) NM_005335.5
Accession Version NM_005335.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-17
Organism Homo sapiens(human)
Product hematopoietic lineage cell-specific protein isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC427610.1, BY994733.1, BP338037.1, BC016758.2, AI091876.1 and BQ002360.1. On May 17, 2014 this sequence version replaced NM_005335.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.130028.1, SRR1163657.362337.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTACT CTCGTGGCTT TGGTGGCCGG 
TACGGGGTGG AGAAGGATAA ATGGGACAAA GCAGCTCTGG GATATGACTA CAAGGGAGAG 
ACGGAGAAAC ACGAGTCCCA GAGAGATTAT GCCAAGGGCT TTGGTGGCCA GTATGGAATC 
CAGAAGGACC GAGTGGATAA GAGCGCTGTC GGCTTCAATG AAATGGAGGC CCCGACCACA 
GCTTATAAGA AGACGACGCC CATAGAAGCC GCTTCTAGTG GTACCCGTGG GCTGAAGGCG 
AAATTTGAGT CCATGGCTGA GGAGAAGAGG AAGCGAGAGG AAGAGGAGAA GGCACAGCAG 
GTGGCCAGGA GGCAACAGGA GCGAAAGGCT GTGACAAAGA GGAGCCCTGA GGCTCCACAG 
CCAGTGATAG CTATGGAAGA GCCAGCAGTA CCGGCCCCAC TGCCCAAGAA AATCTCCTCA 
GAGGCCTGGC CTCCAGTTGG GACTCCTCCA TCATCAGAGT CTGAGCCTGT GAGAACCAGC 
AGGGAACACC CAGTGCCCTT GCTGCCCATT AGGCAGACTC TCCCGGAGGA CAATGAGGAG 
CCCCCAGCTC TGCCCCCTAG GACTCTGGAA GGCCTCCAGG TGGAGGAAGA GCCAGTGTAC 
GAAGCAGAGC CTGAGCCTGA GCCCGAGCCT GAGCCCGAGC CTGAGAATGA CTATGAGGAC 
GTTGAGGAGA TGGACAGGCA TGAGCAGGAG GATGAACCAG AGGGGGACTA TGAGGAGGTG 
CTCGAGCCTG AAGATTCTTC TTTTTCTTCT GCTCTGGCTG GATCATCAGG CTGCCCGGCT 
GGGGCTGGGG CTGGGGCTGT GGCTCTGGGG ATCTCAGCTG TGGCTCTATA TGATTACCAA 
GGAGAGGGAA GTGATGAGCT TTCCTTTGAT CCGGACGACG TAATCACTGA CATTGAGATG 
GTGGACGAGG GCTGGTGGCG GGGACGTTGC CATGGCCACT TTGGACTCTT CCCTGCAAAT 
TATGTCAAGC TTCTGGAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005326.2
CDS130..1590
Translation

Target ORF information:

RefSeq Version NM_005335.5
Organism Homo sapiens(human)
Definition Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_005335.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTACT CTCGTGGCTT TGGTGGCCGG 
TACGGGGTGG AGAAGGATAA ATGGGACAAA GCAGCTCTGG GATATGACTA CAAGGGAGAG 
ACGGAGAAAC ACGAGTCCCA GAGAGATTAT GCCAAGGGCT TTGGTGGCCA GTATGGAATC 
CAGAAGGACC GAGTGGATAA GAGCGCTGTC GGCTTCAATG AAATGGAGGC CCCGACCACA 
GCTTATAAGA AGACGACGCC CATAGAAGCC GCTTCTAGTG GTACCCGTGG GCTGAAGGCG 
AAATTTGAGT CCATGGCTGA GGAGAAGAGG AAGCGAGAGG AAGAGGAGAA GGCACAGCAG 
GTGGCCAGGA GGCAACAGGA GCGAAAGGCT GTGACAAAGA GGAGCCCTGA GGCTCCACAG 
CCAGTGATAG CTATGGAAGA GCCAGCAGTA CCGGCCCCAC TGCCCAAGAA AATCTCCTCA 
GAGGCCTGGC CTCCAGTTGG GACTCCTCCA TCATCAGAGT CTGAGCCTGT GAGAACCAGC 
AGGGAACACC CAGTGCCCTT GCTGCCCATT AGGCAGACTC TCCCGGAGGA CAATGAGGAG 
CCCCCAGCTC TGCCCCCTAG GACTCTGGAA GGCCTCCAGG TGGAGGAAGA GCCAGTGTAC 
GAAGCAGAGC CTGAGCCTGA GCCCGAGCCT GAGCCCGAGC CTGAGAATGA CTATGAGGAC 
GTTGAGGAGA TGGACAGGCA TGAGCAGGAG GATGAACCAG AGGGGGACTA TGAGGAGGTG 
CTCGAGCCTG AAGATTCTTC TTTTTCTTCT GCTCTGGCTG GATCATCAGG CTGCCCGGCT 
GGGGCTGGGG CTGGGGCTGT GGCTCTGGGG ATCTCAGCTG TGGCTCTATA TGATTACCAA 
GGAGAGGGAA GTGATGAGCT TTCCTTTGAT CCGGACGACG TAATCACTGA CATTGAGATG 
GTGGACGAGG GCTGGTGGCG GGGACGTTGC CATGGCCACT TTGGACTCTT CCCTGCAAAT 
TATGTCAAGC TTCTGGAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu107365
Clone ID Related Accession (Same CDS sequence) NM_005335.6
Accession Version NM_005335.6 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-26
Organism Homo sapiens(human)
Product hematopoietic lineage cell-specific protein isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133750.2. On Nov 23, 2018 this sequence version replaced NM_005335.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.362337.1, SRR3476690.455893.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000314583.8/ ENSP00000320176.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTACT CTCGTGGCTT TGGTGGCCGG 
TACGGGGTGG AGAAGGATAA ATGGGACAAA GCAGCTCTGG GATATGACTA CAAGGGAGAG 
ACGGAGAAAC ACGAGTCCCA GAGAGATTAT GCCAAGGGCT TTGGTGGCCA GTATGGAATC 
CAGAAGGACC GAGTGGATAA GAGCGCTGTC GGCTTCAATG AAATGGAGGC CCCGACCACA 
GCTTATAAGA AGACGACGCC CATAGAAGCC GCTTCTAGTG GTACCCGTGG GCTGAAGGCG 
AAATTTGAGT CCATGGCTGA GGAGAAGAGG AAGCGAGAGG AAGAGGAGAA GGCACAGCAG 
GTGGCCAGGA GGCAACAGGA GCGAAAGGCT GTGACAAAGA GGAGCCCTGA GGCTCCACAG 
CCAGTGATAG CTATGGAAGA GCCAGCAGTA CCGGCCCCAC TGCCCAAGAA AATCTCCTCA 
GAGGCCTGGC CTCCAGTTGG GACTCCTCCA TCATCAGAGT CTGAGCCTGT GAGAACCAGC 
AGGGAACACC CAGTGCCCTT GCTGCCCATT AGGCAGACTC TCCCGGAGGA CAATGAGGAG 
CCCCCAGCTC TGCCCCCTAG GACTCTGGAA GGCCTCCAGG TGGAGGAAGA GCCAGTGTAC 
GAAGCAGAGC CTGAGCCTGA GCCCGAGCCT GAGCCCGAGC CTGAGAATGA CTATGAGGAC 
GTTGAGGAGA TGGACAGGCA TGAGCAGGAG GATGAACCAG AGGGGGACTA TGAGGAGGTG 
CTCGAGCCTG AAGATTCTTC TTTTTCTTCT GCTCTGGCTG GATCATCAGG CTGCCCGGCT 
GGGGCTGGGG CTGGGGCTGT GGCTCTGGGG ATCTCAGCTG TGGCTGTATA TGATTACCAA 
GGAGAGGGAA GTGATGAGCT TTCCTTTGAT CCGGACGACG TAATCACTGA CATTGAGATG 
GTGGACGAGG GCTGGTGGCG GGGACGTTGC CATGGCCACT TTGGACTCTT CCCTGCAAAT 
TATGTCAAGC TTCTGGAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005326.3
CDS85..1545
Translation

Target ORF information:

RefSeq Version NM_005335.6
Organism Homo sapiens(human)
Definition Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_005335.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTACT CTCGTGGCTT TGGTGGCCGG 
TACGGGGTGG AGAAGGATAA ATGGGACAAA GCAGCTCTGG GATATGACTA CAAGGGAGAG 
ACGGAGAAAC ACGAGTCCCA GAGAGATTAT GCCAAGGGCT TTGGTGGCCA GTATGGAATC 
CAGAAGGACC GAGTGGATAA GAGCGCTGTC GGCTTCAATG AAATGGAGGC CCCGACCACA 
GCTTATAAGA AGACGACGCC CATAGAAGCC GCTTCTAGTG GTACCCGTGG GCTGAAGGCG 
AAATTTGAGT CCATGGCTGA GGAGAAGAGG AAGCGAGAGG AAGAGGAGAA GGCACAGCAG 
GTGGCCAGGA GGCAACAGGA GCGAAAGGCT GTGACAAAGA GGAGCCCTGA GGCTCCACAG 
CCAGTGATAG CTATGGAAGA GCCAGCAGTA CCGGCCCCAC TGCCCAAGAA AATCTCCTCA 
GAGGCCTGGC CTCCAGTTGG GACTCCTCCA TCATCAGAGT CTGAGCCTGT GAGAACCAGC 
AGGGAACACC CAGTGCCCTT GCTGCCCATT AGGCAGACTC TCCCGGAGGA CAATGAGGAG 
CCCCCAGCTC TGCCCCCTAG GACTCTGGAA GGCCTCCAGG TGGAGGAAGA GCCAGTGTAC 
GAAGCAGAGC CTGAGCCTGA GCCCGAGCCT GAGCCCGAGC CTGAGAATGA CTATGAGGAC 
GTTGAGGAGA TGGACAGGCA TGAGCAGGAG GATGAACCAG AGGGGGACTA TGAGGAGGTG 
CTCGAGCCTG AAGATTCTTC TTTTTCTTCT GCTCTGGCTG GATCATCAGG CTGCCCGGCT 
GGGGCTGGGG CTGGGGCTGT GGCTCTGGGG ATCTCAGCTG TGGCTGTATA TGATTACCAA 
GGAGAGGGAA GTGATGAGCT TTCCTTTGAT CCGGACGACG TAATCACTGA CATTGAGATG 
GTGGACGAGG GCTGGTGGCG GGGACGTTGC CATGGCCACT TTGGACTCTT CCCTGCAAAT 
TATGTCAAGC TTCTGGAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu108219
Clone ID Related Accession (Same CDS sequence) NM_001292041.2
Accession Version NM_001292041.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-27
Organism Homo sapiens(human)
Product hematopoietic lineage cell-specific protein isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133750.2. On May 31, 2019 this sequence version replaced NM_001292041.1. Transcript Variant: This variant (2) lacks an exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298696.1, SRR1163657.191831.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTATG CCAAGGGCTT TGGTGGCCAG 
TATGGAATCC AGAAGGACCG AGTGGATAAG AGCGCTGTCG GCTTCAATGA AATGGAGGCC 
CCGACCACAG CTTATAAGAA GACGACGCCC ATAGAAGCCG CTTCTAGTGG TACCCGTGGG 
CTGAAGGCGA AATTTGAGTC CATGGCTGAG GAGAAGAGGA AGCGAGAGGA AGAGGAGAAG 
GCACAGCAGG TGGCCAGGAG GCAACAGGAG CGAAAGGCTG TGACAAAGAG GAGCCCTGAG 
GCTCCACAGC CAGTGATAGC TATGGAAGAG CCAGCAGTAC CGGCCCCACT GCCCAAGAAA 
ATCTCCTCAG AGGCCTGGCC TCCAGTTGGG ACTCCTCCAT CATCAGAGTC TGAGCCTGTG 
AGAACCAGCA GGGAACACCC AGTGCCCTTG CTGCCCATTA GGCAGACTCT CCCGGAGGAC 
AATGAGGAGC CCCCAGCTCT GCCCCCTAGG ACTCTGGAAG GCCTCCAGGT GGAGGAAGAG 
CCAGTGTACG AAGCAGAGCC TGAGCCTGAG CCCGAGCCTG AGCCCGAGCC TGAGAATGAC 
TATGAGGACG TTGAGGAGAT GGACAGGCAT GAGCAGGAGG ATGAACCAGA GGGGGACTAT 
GAGGAGGTGC TCGAGCCTGA AGATTCTTCT TTTTCTTCTG CTCTGGCTGG ATCATCAGGC 
TGCCCGGCTG GGGCTGGGGC TGGGGCTGTG GCTCTGGGGA TCTCAGCTGT GGCTGTATAT 
GATTACCAAG GAGAGGGAAG TGATGAGCTT TCCTTTGATC CGGACGACGT AATCACTGAC 
ATTGAGATGG TGGACGAGGG CTGGTGGCGG GGACGTTGCC ATGGCCACTT TGGACTCTTC 
CCTGCAAATT ATGTCAAGCT TCTGGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278970.2
CDS85..1434
Translation

Target ORF information:

RefSeq Version NM_001292041.2
Organism Homo sapiens(human)
Definition Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001292041.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTATG CCAAGGGCTT TGGTGGCCAG 
TATGGAATCC AGAAGGACCG AGTGGATAAG AGCGCTGTCG GCTTCAATGA AATGGAGGCC 
CCGACCACAG CTTATAAGAA GACGACGCCC ATAGAAGCCG CTTCTAGTGG TACCCGTGGG 
CTGAAGGCGA AATTTGAGTC CATGGCTGAG GAGAAGAGGA AGCGAGAGGA AGAGGAGAAG 
GCACAGCAGG TGGCCAGGAG GCAACAGGAG CGAAAGGCTG TGACAAAGAG GAGCCCTGAG 
GCTCCACAGC CAGTGATAGC TATGGAAGAG CCAGCAGTAC CGGCCCCACT GCCCAAGAAA 
ATCTCCTCAG AGGCCTGGCC TCCAGTTGGG ACTCCTCCAT CATCAGAGTC TGAGCCTGTG 
AGAACCAGCA GGGAACACCC AGTGCCCTTG CTGCCCATTA GGCAGACTCT CCCGGAGGAC 
AATGAGGAGC CCCCAGCTCT GCCCCCTAGG ACTCTGGAAG GCCTCCAGGT GGAGGAAGAG 
CCAGTGTACG AAGCAGAGCC TGAGCCTGAG CCCGAGCCTG AGCCCGAGCC TGAGAATGAC 
TATGAGGACG TTGAGGAGAT GGACAGGCAT GAGCAGGAGG ATGAACCAGA GGGGGACTAT 
GAGGAGGTGC TCGAGCCTGA AGATTCTTCT TTTTCTTCTG CTCTGGCTGG ATCATCAGGC 
TGCCCGGCTG GGGCTGGGGC TGGGGCTGTG GCTCTGGGGA TCTCAGCTGT GGCTGTATAT 
GATTACCAAG GAGAGGGAAG TGATGAGCTT TCCTTTGATC CGGACGACGT AATCACTGAC 
ATTGAGATGG TGGACGAGGG CTGGTGGCGG GGACGTTGCC ATGGCCACTT TGGACTCTTC 
CCTGCAAATT ATGTCAAGCT TCTGGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24656
Clone ID Related Accession (Same CDS sequence) NM_001292041.1
Accession Version NM_001292041.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-29
Organism Homo sapiens(human)
Product hematopoietic lineage cell-specific protein isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC427610.1, BY994733.1, BP338037.1, BC016758.2, AI091876.1 and BQ002360.1. Transcript Variant: This variant (2) lacks an exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.191831.1, AK298696.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTATG CCAAGGGCTT TGGTGGCCAG 
TATGGAATCC AGAAGGACCG AGTGGATAAG AGCGCTGTCG GCTTCAATGA AATGGAGGCC 
CCGACCACAG CTTATAAGAA GACGACGCCC ATAGAAGCCG CTTCTAGTGG TACCCGTGGG 
CTGAAGGCGA AATTTGAGTC CATGGCTGAG GAGAAGAGGA AGCGAGAGGA AGAGGAGAAG 
GCACAGCAGG TGGCCAGGAG GCAACAGGAG CGAAAGGCTG TGACAAAGAG GAGCCCTGAG 
GCTCCACAGC CAGTGATAGC TATGGAAGAG CCAGCAGTAC CGGCCCCACT GCCCAAGAAA 
ATCTCCTCAG AGGCCTGGCC TCCAGTTGGG ACTCCTCCAT CATCAGAGTC TGAGCCTGTG 
AGAACCAGCA GGGAACACCC AGTGCCCTTG CTGCCCATTA GGCAGACTCT CCCGGAGGAC 
AATGAGGAGC CCCCAGCTCT GCCCCCTAGG ACTCTGGAAG GCCTCCAGGT GGAGGAAGAG 
CCAGTGTACG AAGCAGAGCC TGAGCCTGAG CCCGAGCCTG AGCCCGAGCC TGAGAATGAC 
TATGAGGACG TTGAGGAGAT GGACAGGCAT GAGCAGGAGG ATGAACCAGA GGGGGACTAT 
GAGGAGGTGC TCGAGCCTGA AGATTCTTCT TTTTCTTCTG CTCTGGCTGG ATCATCAGGC 
TGCCCGGCTG GGGCTGGGGC TGGGGCTGTG GCTCTGGGGA TCTCAGCTGT GGCTCTATAT 
GATTACCAAG GAGAGGGAAG TGATGAGCTT TCCTTTGATC CGGACGACGT AATCACTGAC 
ATTGAGATGG TGGACGAGGG CTGGTGGCGG GGACGTTGCC ATGGCCACTT TGGACTCTTC 
CCTGCAAATT ATGTCAAGCT TCTGGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278970.1
CDS130..1479
Translation

Target ORF information:

RefSeq Version NM_001292041.1
Organism Homo sapiens(human)
Definition Homo sapiens hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001292041.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTGGAAGT CTGTAGTGGG CCATGATGTG TCTGTTTCCG TGGAGACCCA GGGTGATGAT 
TGGGACACAG ATCCTGACTT TGTGAATGAC ATCTCTGAAA AGGAGCAACG ATGGGGAGCC 
AAGACCATCG AGGGGTCTGG ACGCACAGAA CACATCAACA TCCACCAGCT GAGGAACAAA 
GTATCAGAGG AGCATGATGT TCTCAGGAAG AAAGAGATGG AGTCAGGGCC CAAAGCATCC 
CATGGCTATG GAGGTCGGTT TGGAGTAGAA AGAGACCGAA TGGACAAGAG TGCAGTGGGC 
CATGAGTATG TTGCCGAGGT GGAGAAGCAC TCTTCTCAGA CGGATGCTGC CAAAGGCTTT 
GGGGGCAAGT ACGGAGTTGA GAGGGACAGG GCAGACAAGT CAGCAGTCGG CTTTGATTAT 
AAAGGAGAAG TGGAGAAGCA CACATCTCAG AAAGATTATG CCAAGGGCTT TGGTGGCCAG 
TATGGAATCC AGAAGGACCG AGTGGATAAG AGCGCTGTCG GCTTCAATGA AATGGAGGCC 
CCGACCACAG CTTATAAGAA GACGACGCCC ATAGAAGCCG CTTCTAGTGG TACCCGTGGG 
CTGAAGGCGA AATTTGAGTC CATGGCTGAG GAGAAGAGGA AGCGAGAGGA AGAGGAGAAG 
GCACAGCAGG TGGCCAGGAG GCAACAGGAG CGAAAGGCTG TGACAAAGAG GAGCCCTGAG 
GCTCCACAGC CAGTGATAGC TATGGAAGAG CCAGCAGTAC CGGCCCCACT GCCCAAGAAA 
ATCTCCTCAG AGGCCTGGCC TCCAGTTGGG ACTCCTCCAT CATCAGAGTC TGAGCCTGTG 
AGAACCAGCA GGGAACACCC AGTGCCCTTG CTGCCCATTA GGCAGACTCT CCCGGAGGAC 
AATGAGGAGC CCCCAGCTCT GCCCCCTAGG ACTCTGGAAG GCCTCCAGGT GGAGGAAGAG 
CCAGTGTACG AAGCAGAGCC TGAGCCTGAG CCCGAGCCTG AGCCCGAGCC TGAGAATGAC 
TATGAGGACG TTGAGGAGAT GGACAGGCAT GAGCAGGAGG ATGAACCAGA GGGGGACTAT 
GAGGAGGTGC TCGAGCCTGA AGATTCTTCT TTTTCTTCTG CTCTGGCTGG ATCATCAGGC 
TGCCCGGCTG GGGCTGGGGC TGGGGCTGTG GCTCTGGGGA TCTCAGCTGT GGCTCTATAT 
GATTACCAAG GAGAGGGAAG TGATGAGCTT TCCTTTGATC CGGACGACGT AATCACTGAC 
ATTGAGATGG TGGACGAGGG CTGGTGGCGG GGACGTTGCC ATGGCCACTT TGGACTCTTC 
CCTGCAAATT ATGTCAAGCT TCTGGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells.
Leukemia31(12)2615-2622(2017 Dec)
Hasan MK,Yu J,Chen L,Cui B,Widhopf Ii GF,Rassenti L,Shen Z,Briggs SP,Kipps TJ


book

Exome sequencing reveals recurrent germ line variants in patients with familial Waldenstr?m macroglobulinemia.
Blood127(21)2598-606(2016 05)
Roccaro AM,Sacco A,Shi J,Chiarini M,Perilla-Glen A,Manier S,Glavey S,Aljawai Y,Mishima Y,Kawano Y,Moschetta M,Correll M,Improgo MR,Brown JR,Imberti L,Rossi G,Castillo JJ,Treon SP,Freedman ML,Van Allen EM,Hide W,Hiller E,Rainville I,Ghobrial IM


book

SDF1?-induced interaction of the adapter proteins Nck and HS1 facilitates actin polymerization and migration in T?cells.
European journal of immunology45(2)551-61(2015 Feb)
Lettau M,Kabelitz D,Janssen O


book

Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors.
Journal of proteomics78231-44(2013 Jan)
Habif G,Grasset MF,Kieffer-Jaquinod S,Kuhn L,Mouchiroud G,Gobert-Gosse S


book

Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis.
Nature medicine18(10)1550-9(2012 Oct)
Skokowa J,Klimiankou M,Klimenkova O,Lan D,Gupta K,Hussein K,Carrizosa E,Kusnetsova I,Li Z,Sustmann C,Ganser A,Zeidler C,Kreipe HH,Burkhardt J,Grosschedl R,Welte K