HMGCS1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following HMGCS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMGCS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu01309 NM_001324220.2
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_001324219.2
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_002130.8
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_001330663.2
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_001364188.2
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_001098272.3
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu39317 NM_001324224.2
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu39317 NM_001324223.2
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu39317 NM_001324222.2
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu58268 XM_024446038.1
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu58268 XM_011514036.3
Latest version!
Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu39317 NM_001324222.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu01309 NM_001330663.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu39317 NM_001324224.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu39317 NM_001324223.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu01309 NM_001324219.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_001364188.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_001098272.2 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu58268 XM_011514036.2 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu01309 NM_002130.7 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu01309 NM_001324220.1 Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001324220.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, BJ995153.1, AK315593.1 and AC114947.2. On Apr 28, 2016 this sequence version replaced XM_011514034.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.61106.1, SRR1803611.194174.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311149.1
    CDS247..1809
    Translation

    Target ORF information:

    RefSeq Version NM_001324220.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001330663.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 31, 2019 this sequence version replaced NM_001330663.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.96054.1, SRR1660807.126299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317592.1
    CDS230..1792
    Translation

    Target ORF information:

    RefSeq Version NM_001330663.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330663.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_002130.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 17, 2012 this sequence version replaced NM_002130.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.158150.1, SRR1803613.257271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002121.4
    CDS168..1730
    Translation

    Target ORF information:

    RefSeq Version NM_002130.7
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002130.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001098272.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-25
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK315593.1 and AC114947.2. On Nov 22, 2018 this sequence version replaced NM_001098272.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.216745.1, SRR1803615.11048.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000325110.11/ ENSP00000322706.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091742.1
    CDS127..1689
    Translation

    Target ORF information:

    RefSeq Version NM_001098272.3
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098272.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001330663.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On Sep 1, 2016 this sequence version replaced XM_017009412.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.96054.1, SRR1660807.126299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317592.1
    CDS346..1908
    Translation

    Target ORF information:

    RefSeq Version NM_001330663.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330663.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001324219.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA801434.1, AK315593.1 and AC114947.2. On May 31, 2019 this sequence version replaced NM_001324219.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.2816.1, SRR1803616.112840.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267755, SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311148.1
    CDS171..1733
    Translation

    Target ORF information:

    RefSeq Version NM_001324219.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324219.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001364188.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 31, 2019 this sequence version replaced NM_001364188.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.42317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001351117.1
    CDS250..1812
    Translation

    Target ORF information:

    RefSeq Version NM_001364188.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001364188.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001324219.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA801434.1, AK315593.1 and AC114947.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.252369.1, SRR1803616.2816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267755, SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311148.1
    CDS214..1776
    Translation

    Target ORF information:

    RefSeq Version NM_001324219.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001364188.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.42317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001351117.1
    CDS250..1812
    Translation

    Target ORF information:

    RefSeq Version NM_001364188.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001364188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001098272.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. On May 16, 2012 this sequence version replaced NM_001098272.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.11048.1, SRR1660809.42847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091742.1
    CDS227..1789
    Translation

    Target ORF information:

    RefSeq Version NM_001098272.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098272.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_002130.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114947.2. On May 31, 2019 this sequence version replaced NM_002130.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.92027.1, SRR1660807.236422.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002121.4
    CDS68..1630
    Translation

    Target ORF information:

    RefSeq Version NM_002130.8
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002130.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01309
    Clone ID Related Accession (Same CDS sequence) NM_001324220.1 , NM_001330663.2 , NM_002130.7 , NM_001098272.3 , NM_001330663.1 , NM_001324219.2 , NM_001364188.2 , NM_001324219.1 , NM_001364188.1 , NM_001098272.2 , NM_002130.8 , NM_001324220.2
    Accession Version NM_001324220.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BJ995153.1, AK315593.1 and AC114947.2. On Jun 1, 2019 this sequence version replaced NM_001324220.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.163703.1, SRR1660803.231163.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311149.1
    CDS147..1709
    Translation

    Target ORF information:

    RefSeq Version NM_001324220.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324220.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGA CGGTATGCCC TGGTAGTTGC AGGAGATATT
    GCTGTATATG CCACAGGAAA TGCTAGACCT ACAGGTGGAG TTGGAGCAGT AGCTCTGCTA
    ATTGGGCCAA ATGCTCCTTT AATTTTTGAA CGAGGGCTTC GTGGGACACA TATGCAACAT
    GCCTATGATT TTTACAAGCC TGATATGCTA TCTGAATATC CTATAGTAGA TGGAAAACTC
    TCCATACAGT GCTACCTCAG TGCATTAGAC CGCTGCTATT CTGTCTACTG CAAAAAGATC
    CATGCCCAGT GGCAGAAAGA GGGAAATGAT AAAGATTTTA CCTTGAATGA TTTTGGCTTC
    ATGATCTTTC ACTCACCATA TTGTAAACTG GTTCAGAAAT CTCTAGCTCG GATGTTGCTG
    AATGACTTCC TTAATGACCA GAATAGAGAT AAAAATAGTA TCTATAGTGG CCTGGAAGCC
    TTTGGGGATG TTAAATTAGA AGACACCTAC TTTGATAGAG ATGTGGAGAA GGCATTTATG
    AAGGCTAGCT CTGAACTCTT CAGTCAGAAA ACAAAGGCAT CTTTACTTGT ATCAAATCAA
    AATGGAAATA TGTACACATC TTCAGTATAT GGTTCCCTTG CATCTGTTCT AGCACAGTAC
    TCACCTCAGC AATTAGCAGG GAAGAGAATT GGAGTGTTTT CTTATGGTTC TGGTTTGGCT
    GCCACTCTGT ACTCTCTTAA AGTCACACAA GATGCTACAC CGGGGTCTGC TCTTGATAAA
    ATAACAGCAA GTTTATGTGA TCTTAAATCA AGGCTTGATT CAAGAACTGG TGTGGCACCA
    GATGTCTTCG CTGAAAACAT GAAGCTCAGA GAGGACACCC ATCATTTGGT CAACTATATT
    CCCCAGGGTT CAATAGATTC ACTCTTTGAA GGAACGTGGT ACTTAGTTAG GGTGGATGAA
    AAGCACAGAA GAACTTACGC TCGGCGTCCC ACTCCAAATG ATGACACTTT GGATGAAGGA
    GTAGGACTTG TGCATTCAAA CATAGCAACT GAGCATATTC CAAGCCCTGC CAAGAAAGTA
    CCAAGACTCC CTGCCACAGC AGCAGAACCT GAAGCAGCTG TCATTAGTAA TGGGGAACAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39317
    Clone ID Related Accession (Same CDS sequence) NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
    Accession Version NM_001324222.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK315593.1 and AC114947.2. On Jun 1, 2019 this sequence version replaced NM_001324222.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.84804.1, SRR1803613.16109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311151.1
    CDS127..1563
    Translation

    Target ORF information:

    RefSeq Version NM_001324222.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324222.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39317
    Clone ID Related Accession (Same CDS sequence) NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
    Accession Version NM_001324224.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, BJ995153.1, AK315593.1 and AC114947.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.92218.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311153.1
    CDS247..1683
    Translation

    Target ORF information:

    RefSeq Version NM_001324224.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324224.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39317
    Clone ID Related Accession (Same CDS sequence) NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
    Accession Version NM_001324222.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. On Apr 28, 2016 this sequence version replaced XM_011514035.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.84804.1, SRR1803613.16109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311151.1
    CDS227..1663
    Translation

    Target ORF information:

    RefSeq Version NM_001324222.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39317
    Clone ID Related Accession (Same CDS sequence) NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
    Accession Version NM_001324223.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. On May 31, 2019 this sequence version replaced NM_001324223.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.272420.1, SRR1660803.103675.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311152.1
    CDS68..1504
    Translation

    Target ORF information:

    RefSeq Version NM_001324223.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324223.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39317
    Clone ID Related Accession (Same CDS sequence) NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
    Accession Version NM_001324224.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BJ995153.1, AK315593.1 and AC114947.2. On May 31, 2019 this sequence version replaced NM_001324224.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.92218.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311153.1
    CDS147..1583
    Translation

    Target ORF information:

    RefSeq Version NM_001324224.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324224.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39317
    Clone ID Related Accession (Same CDS sequence) NM_001324222.2 , NM_001324224.1 , NM_001324222.1 , NM_001324223.2 , NM_001324224.2 , NM_001324223.1
    Accession Version NM_001324223.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB074885.1, AK315593.1 and AC114947.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.284767.1, SRR1803617.187120.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267761 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311152.1
    CDS168..1604
    Translation

    Target ORF information:

    RefSeq Version NM_001324223.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTGGAT CACTTCCTTT GAATGCAGAA GCTTGCTGGC CAAAAGATGT GGGAATTGTT 
    GCCCTTGAGA TCTATTTTCC TTCTCAATAT GTTGATCAAG CAGAGTTGGA AAAATATGAT
    GGTGTAGATG CTGGAAAGTA TACCATTGGC TTGGGCCAGG CCAAGATGGG CTTCTGCACA
    GATAGAGAAG ATATTAACTC TCTTTGCATG ACTGTGGTTC AGAATCTTAT GGAGAGAAAT
    AACCTTTCCT ATGATTGCAT TGGGCGGCTG GAAGTTGGAA CAGAGACAAT CATCGACAAA
    TCAAAGTCTG TGAAGACTAA TTTGATGCAG CTGTTTGAAG AGTCTGGGAA TACAGATATA
    GAAGGAATCG ACACAACTAA TGCATGCTAT GGAGGCACAG CTGCTGTCTT CAATGCTGTT
    AACTGGATTG AGTCCAGCTC TTGGGATGGG CTTCGTGGGA CACATATGCA ACATGCCTAT
    GATTTTTACA AGCCTGATAT GCTATCTGAA TATCCTATAG TAGATGGAAA ACTCTCCATA
    CAGTGCTACC TCAGTGCATT AGACCGCTGC TATTCTGTCT ACTGCAAAAA GATCCATGCC
    CAGTGGCAGA AAGAGGGAAA TGATAAAGAT TTTACCTTGA ATGATTTTGG CTTCATGATC
    TTTCACTCAC CATATTGTAA ACTGGTTCAG AAATCTCTAG CTCGGATGTT GCTGAATGAC
    TTCCTTAATG ACCAGAATAG AGATAAAAAT AGTATCTATA GTGGCCTGGA AGCCTTTGGG
    GATGTTAAAT TAGAAGACAC CTACTTTGAT AGAGATGTGG AGAAGGCATT TATGAAGGCT
    AGCTCTGAAC TCTTCAGTCA GAAAACAAAG GCATCTTTAC TTGTATCAAA TCAAAATGGA
    AATATGTACA CATCTTCAGT ATATGGTTCC CTTGCATCTG TTCTAGCACA GTACTCACCT
    CAGCAATTAG CAGGGAAGAG AATTGGAGTG TTTTCTTATG GTTCTGGTTT GGCTGCCACT
    CTGTACTCTC TTAAAGTCAC ACAAGATGCT ACACCGGGGT CTGCTCTTGA TAAAATAACA
    GCAAGTTTAT GTGATCTTAA ATCAAGGCTT GATTCAAGAA CTGGTGTGGC ACCAGATGTC
    TTCGCTGAAA ACATGAAGCT CAGAGAGGAC ACCCATCATT TGGTCAACTA TATTCCCCAG
    GGTTCAATAG ATTCACTCTT TGAAGGAACG TGGTACTTAG TTAGGGTGGA TGAAAAGCAC
    AGAAGAACTT ACGCTCGGCG TCCCACTCCA AATGATGACA CTTTGGATGA AGGAGTAGGA
    CTTGTGCATT CAAACATAGC AACTGAGCAT ATTCCAAGCC CTGCCAAGAA AGTACCAAGA
    CTCCCTGCCA CAGCAGCAGA ACCTGAAGCA GCTGTCATTA GTAATGGGGA ACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58268
    Clone ID Related Accession (Same CDS sequence) XM_011514036.3 , XM_024446038.1 , XM_011514036.2
    Accession Version XM_011514036.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
    GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC
    AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT
    TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG
    ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC
    CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG
    GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA
    TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA
    GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT
    GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT
    CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT
    GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC
    AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG
    GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG
    GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC
    AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT
    GGGGAACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512338.1
    CDS1587..2558
    Translation

    Target ORF information:

    RefSeq Version XM_011514036.3
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514036.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
    GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC
    AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT
    TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG
    ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC
    CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG
    GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA
    TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA
    GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT
    GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT
    CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT
    GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC
    AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG
    GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG
    GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC
    AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT
    GGGGAACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58268
    Clone ID Related Accession (Same CDS sequence) XM_011514036.3 , XM_024446038.1 , XM_011514036.2
    Accession Version XM_024446038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
    GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC
    AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT
    TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG
    ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC
    CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG
    GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA
    TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA
    GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT
    GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT
    CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT
    GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC
    AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG
    GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG
    GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC
    AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT
    GGGGAACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024301806.1
    CDS1636..2607
    Translation

    Target ORF information:

    RefSeq Version XM_024446038.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
    GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC
    AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT
    TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG
    ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC
    CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG
    GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA
    TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA
    GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT
    GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT
    CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT
    GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC
    AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG
    GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG
    GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC
    AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT
    GGGGAACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58268
    Clone ID Related Accession (Same CDS sequence) XM_011514036.3 , XM_024446038.1 , XM_011514036.2
    Accession Version XM_011514036.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product hydroxymethylglutaryl-CoA synthase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
    GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC
    AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT
    TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG
    ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC
    CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG
    GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA
    TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA
    GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT
    GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT
    CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT
    GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC
    AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG
    GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG
    GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC
    AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT
    GGGGAACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512338.1
    CDS624..1595
    Translation

    Target ORF information:

    RefSeq Version XM_011514036.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514036.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAACATG CCTATGATTT TTACAAGCCT GATATGCTAT CTGAATATCC TATAGTAGAT 
    GGAAAACTCT CCATACAGTG CTACCTCAGT GCATTAGACC GCTGCTATTC TGTCTACTGC
    AAAAAGATCC ATGCCCAGTG GCAGAAAGAG GGAAATGATA AAGATTTTAC CTTGAATGAT
    TTTGGCTTCA TGATCTTTCA CTCACCATAT TGTAAACTGG TTCAGAAATC TCTAGCTCGG
    ATGTTGCTGA ATGACTTCCT TAATGACCAG AATAGAGATA AAAATAGTAT CTATAGTGGC
    CTGGAAGCCT TTGGGGATGT TAAATTAGAA GACACCTACT TTGATAGAGA TGTGGAGAAG
    GCATTTATGA AGGCTAGCTC TGAACTCTTC AGTCAGAAAA CAAAGGCATC TTTACTTGTA
    TCAAATCAAA ATGGAAATAT GTACACATCT TCAGTATATG GTTCCCTTGC ATCTGTTCTA
    GCACAGTACT CACCTCAGCA ATTAGCAGGG AAGAGAATTG GAGTGTTTTC TTATGGTTCT
    GGTTTGGCTG CCACTCTGTA CTCTCTTAAA GTCACACAAG ATGCTACACC GGGGTCTGCT
    CTTGATAAAA TAACAGCAAG TTTATGTGAT CTTAAATCAA GGCTTGATTC AAGAACTGGT
    GTGGCACCAG ATGTCTTCGC TGAAAACATG AAGCTCAGAG AGGACACCCA TCATTTGGTC
    AACTATATTC CCCAGGGTTC AATAGATTCA CTCTTTGAAG GAACGTGGTA CTTAGTTAGG
    GTGGATGAAA AGCACAGAAG AACTTACGCT CGGCGTCCCA CTCCAAATGA TGACACTTTG
    GATGAAGGAG TAGGACTTGT GCATTCAAAC ATAGCAACTG AGCATATTCC AAGCCCTGCC
    AAGAAAGTAC CAAGACTCCC TGCCACAGCA GCAGAACCTG AAGCAGCTGT CATTAGTAAT
    GGGGAACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.