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KLK3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol KLK3
Entrez Gene ID 354
Full Name kallikrein-related peptidase 3
Synonyms APS, KLK2A1, PSA, hK3
General protein information
Preferred Names
prostate-specific antigen
Names
prostate-specific antigen
seminin
P-30 antigen
kallikrein-3
semenogelase
gamma-seminoprotein
prostate specific antigen
NP_001025218.1
NP_001025219.1
NP_001025221.1
NP_001639.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.41

Summary Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

176820


mRNA and Protein(s)

mRNA Protein Name
XM_011526923 XP_011525225 prostate-specific antigen isoform X1
XM_011526924 XP_011525226 prostate-specific antigen isoform X2
NM_001648 NP_001639 prostate-specific antigen isoform 1 preproprotein
NM_001030047 NP_001025218 prostate-specific antigen isoform 3 preproprotein
NM_001030048 NP_001025219 prostate-specific antigen isoform 4 preproprotein



Homo sapiens (human) KLK3 NP_001639.1
Macaca mulatta (Rhesus monkey) KLK3 NP_001036241.1
Mus musculus (house mouse) Klk1b8 NP_032483.1
Mus musculus (house mouse) Klk1b16 NP_032480.1
Mus musculus (house mouse) Klk1 NP_034769.4
Mus musculus (house mouse) Klk1b5 NP_032482.1
Mus musculus (house mouse) Klk1b1 NP_034775.1
Mus musculus (house mouse) Klk1b9 NP_034246.1
Mus musculus (house mouse) Klk1b3 NP_032719.1
Mus musculus (house mouse) Klk1b11 NP_034770.1
Mus musculus (house mouse) Klk1b24 NP_034773.1
Mus musculus (house mouse) Klk1b26 NP_034774.1
Mus musculus (house mouse) Klk1b21 NP_034772.1
Mus musculus (house mouse) Klk1b27 NP_064664.1
Mus musculus (house mouse) Klk1b22 NP_034244.1
Mus musculus (house mouse) Klk1b4 NP_035045.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KLK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KLK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu56336 XM_011526923 PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu56337 XM_011526924 PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu23388
NM_001648 Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu23435
NM_001030047 Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$134.50-$188.30
$269.00
OHu23926 NM_001030048 Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu56336
Clone ID Related Accession (Same CDS sequence) XM_011526923
Accession Version XM_011526923.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGGG TGATTCTGGG
GGCCCACTTG TCTGTAATGG TGTGCTTCAA GGTATCACGT CATGGGGCAG TGAACCATGT
GCCCTGCCCG AAAGGCCTTC CCTGTACACC AAGGTGGTGC ATTACCGGAA GTGGATCAAG
GACACCATCG TGGCCAACCC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525225.1
CDS36..839
Misc Feature(1)105..812(+)
Misc Feature(2)108..821(+)
Misc Feature(3)108..110(+)
Misc Feature(4)228..692(+)
Misc Feature(5)672..743(+)
Translation

Target ORF information:

RefSeq Version XM_011526923
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526923

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGGG TGATTCTGGG
GGCCCACTTG TCTGTAATGG TGTGCTTCAA GGTATCACGT CATGGGGCAG TGAACCATGT
GCCCTGCCCG AAAGGCCTTC CCTGTACACC AAGGTGGTGC ATTACCGGAA GTGGATCAAG
GACACCATCG TGGCCAACCC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56337
Clone ID Related Accession (Same CDS sequence) XM_011526924
Accession Version XM_011526924.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 735bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGTG GGTCATTCTG
ATCACCGAAC TGACCATGCC AGCCCTGCCG ATGGTCCTCC ATGGCTCCCT AGTGCCCTGG
AGAGGAGGTG TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525226.1
CDS36..770
Misc Feature(1)105..>680(+)
Misc Feature(2)108..>680(+)
Misc Feature(3)108..110(+)
Misc Feature(4)228..395(+)
Translation

Target ORF information:

RefSeq Version XM_011526924
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526924

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTGTACGCC TGGGCCAGAT GTCTTGACCC CAAAGAAACT TCAGTGTGTG
GACCTCCATG TTATTTCCAA TGACGTGTGT GCGCAAGTTC ACCCTCAGAA GGTGACCAAG
TTCATGCTGT GTGCTGGACG CTGGACAGGG GGCAAAAGCA CCTGCTCGTG GGTCATTCTG
ATCACCGAAC TGACCATGCC AGCCCTGCCG ATGGTCCTCC ATGGCTCCCT AGTGCCCTGG
AGAGGAGGTG TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23388
Clone ID Related Accession (Same CDS sequence) NM_001648
Accession Version NM_001648.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X05332.1. This sequence is a reference standard in the RefSeqGene project. On Aug 12, 2002 this sequence version replaced gi:4502172. Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X05332.1, M26663.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG GGTGATTCTG GGGGCCCACT TGTCTGTAAT
GGTGTGCTTC AAGGTATCAC GTCATGGGGC AGTGAACCAT GTGCCCTGCC CGAAAGGCCT
TCCCTGTACA CCAAGGTGGT GCATTACCGG AAGTGGATCA AGGACACCAT CGTGGCCAAC
CCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001639.1
CDS42..827
Misc Feature(1)111..800(+)
Misc Feature(2)114..809(+)
Misc Feature(3)114..116(+)
Misc Feature(4)234..680(+)
Misc Feature(5)246..248(+)
Misc Feature(6)273..275(+)
Misc Feature(7)282..284(+)
Misc Feature(8)546..548(+)
Misc Feature(9)660..731(+)
Exon (1)1..87
Gene:KLK3
Gene Synonym:
Exon (2)88..247
Gene:KLK3
Gene Synonym:
Exon (3)248..534
Gene:KLK3
Gene Synonym:
Exon (4)535..671
Gene:KLK3
Gene Synonym:
Exon (5)672..1464
Gene:KLK3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001648
Organism Homo sapiens (human)
Definition Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001648

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG GGTGATTCTG GGGGCCCACT TGTCTGTAAT
GGTGTGCTTC AAGGTATCAC GTCATGGGGC AGTGAACCAT GTGCCCTGCC CGAAAGGCCT
TCCCTGTACA CCAAGGTGGT GCATTACCGG AAGTGGATCA AGGACACCAT CGTGGCCAAC
CCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23435
Clone ID Related Accession (Same CDS sequence) NM_001030047
Accession Version NM_001030047.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005307.1, M21896.1 and AC011523.3. Transcript Variant: This variant (3) uses an alternate splice site at the end of a coding exon, that causes a frameshift. The resulting isoform (3) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU932434.1, M21896.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG TGGGTCATTC TGATCACCGA ACTGACCATG
CCAGCCCTGC CGATGGTCCT CCATGGCTCC CTAGTGCCCT GGAGAGGAGG TGTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025218.1
CDS42..758
Misc Feature(1)111..>668(+)
Misc Feature(2)114..>668(+)
Misc Feature(3)114..116(+)
Misc Feature(4)234..401(+)
Exon (1)1..87
Gene:KLK3
Gene Synonym:
Exon (2)88..247
Gene:KLK3
Gene Synonym:
Exon (3)248..534
Gene:KLK3
Gene Synonym:
Exon (4)535..671
Gene:KLK3
Gene Synonym:
Exon (5)672..1906
Gene:KLK3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001030047
Organism Homo sapiens (human)
Definition Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030047

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAACAAA AGCGTGATCT TGCTGGGTCG GCACAGCCTG
TTTCATCCTG AAGACACAGG CCAGGTATTT CAGGTCAGCC ACAGCTTCCC ACACCCGCTC
TACGATATGA GCCTCCTGAA GAATCGATTC CTCAGGCCAG GTGATGACTC CAGCCACGAC
CTCATGCTGC TCCGCCTGTC AGAGCCTGCC GAGCTCACGG ATGCTGTGAA GGTCATGGAC
CTGCCCACCC AGGAGCCAGC ACTGGGGACC ACCTGCTACG CCTCAGGCTG GGGCAGCATT
GAACCAGAGG AGTTCTTGAC CCCAAAGAAA CTTCAGTGTG TGGACCTCCA TGTTATTTCC
AATGACGTGT GTGCGCAAGT TCACCCTCAG AAGGTGACCA AGTTCATGCT GTGTGCTGGA
CGCTGGACAG GGGGCAAAAG CACCTGCTCG TGGGTCATTC TGATCACCGA ACTGACCATG
CCAGCCCTGC CGATGGTCCT CCATGGCTCC CTAGTGCCCT GGAGAGGAGG TGTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23926
Clone ID Related Accession (Same CDS sequence) NM_001030048
Accession Version NM_001030048.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product prostate-specific antigen isoform 4 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005307.1, BQ932072.1 and AC011523.3. Transcript Variant: This variant (4) uses an alternate in-frame splice site at the end of a coding exon. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ932072.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAAGCCA GGTGATGACT CCAGCCACGA CCTCATGCTG
CTCCGCCTGT CAGAGCCTGC CGAGCTCACG GATGCTGTGA AGGTCATGGA CCTGCCCACC
CAGGAGCCAG CACTGGGGAC CACCTGCTAC GCCTCAGGCT GGGGCAGCAT TGAACCAGAG
GAGTTCTTGA CCCCAAAGAA ACTTCAGTGT GTGGACCTCC ATGTTATTTC CAATGACGTG
TGTGCGCAAG TTCACCCTCA GAAGGTGACC AAGTTCATGC TGTGTGCTGG ACGCTGGACA
GGGGGCAAAA GCACCTGCTC GGGTGATTCT GGGGGCCCAC TTGTCTGTAA TGGTGTGCTT
CAAGGTATCA CGTCATGGGG CAGTGAACCA TGTGCCCTGC CCGAAAGGCC TTCCCTGTAC
ACCAAGGTGG TGCATTACCG GAAGTGGATC AAGGACACCA TCGTGGCCAA CCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001025219.1
CDS42..698
Misc Feature(1)111..671(+)
Misc Feature(2)114..680(+)
Misc Feature(3)114..116(+)
Misc Feature(4)234..551(+)
Misc Feature(5)531..602(+)
Exon (1)1..87
Gene:KLK3
Gene Synonym:
Exon (2)88..247
Gene:KLK3
Gene Synonym:
Exon (3)248..405
Gene:KLK3
Gene Synonym:
Exon (4)406..542
Gene:KLK3
Gene Synonym:
Exon (5)543..1335
Gene:KLK3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001030048
Organism Homo sapiens (human)
Definition Homo sapiens kallikrein-related peptidase 3 (KLK3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001030048

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGTGGGTCC CGGTTGTCTT CCTCACCCTG TCCGTGACGT GGATTGGTGC TGCACCCCTC 
ATCCTGTCTC GGATTGTGGG AGGCTGGGAG TGCGAGAAGC ATTCCCAACC CTGGCAGGTG
CTTGTGGCCT CTCGTGGCAG GGCAGTCTGC GGCGGTGTTC TGGTGCACCC CCAGTGGGTC
CTCACAGCTG CCCACTGCAT CAGGAAGCCA GGTGATGACT CCAGCCACGA CCTCATGCTG
CTCCGCCTGT CAGAGCCTGC CGAGCTCACG GATGCTGTGA AGGTCATGGA CCTGCCCACC
CAGGAGCCAG CACTGGGGAC CACCTGCTAC GCCTCAGGCT GGGGCAGCAT TGAACCAGAG
GAGTTCTTGA CCCCAAAGAA ACTTCAGTGT GTGGACCTCC ATGTTATTTC CAATGACGTG
TGTGCGCAAG TTCACCCTCA GAAGGTGACC AAGTTCATGC TGTGTGCTGG ACGCTGGACA
GGGGGCAAAA GCACCTGCTC GGGTGATTCT GGGGGCCCAC TTGTCTGTAA TGGTGTGCTT
CAAGGTATCA CGTCATGGGG CAGTGAACCA TGTGCCCTGC CCGAAAGGCC TTCCCTGTAC
ACCAAGGTGG TGCATTACCG GAAGTGGATC AAGGACACCA TCGTGGCCAA CCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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