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IDO1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol IDO1
Entrez Gene ID 3620
Full Name indoleamine 2,3-dioxygenase 1
Synonyms IDO, IDO-1, INDO
General protein information
Preferred Names
indoleamine 2,3-dioxygenase 1
Names
indoleamine 2,3-dioxygenase 1
indole 2,3-dioxygenase
indolamine 2,3 dioxygenase
indoleamine-pyrrole 2,3-dioxygenase
NP_002155.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8p12-p11

Summary This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]. lac of sum
Disorder MIM:

147435


mRNA and Protein(s)

mRNA Protein Name
NM_002164 NP_002155 indoleamine 2,3-dioxygenase 1



Homo sapiens (human) IDO1 NP_002155.1
Pan troglodytes (chimpanzee) IDO1 XP_001137531.1
Macaca mulatta (Rhesus monkey) IDO1 NP_001070951.1
Canis lupus familiaris (dog) IDO1 XP_532793.1
Bos taurus (cattle) IDO1 NP_001095336.1
Mus musculus (house mouse) Ido1 NP_032350.1
Rattus norvegicus (Norway rat) Ido1 NP_076463.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IDO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20074
NM_002164 Homo sapiens indoleamine 2,3-dioxygenase 1 (IDO1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20074
Clone ID Related Accession (Same CDS sequence) NM_002164
Accession Version NM_002164.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product indoleamine 2,3-dioxygenase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU137351.1, BC027882.1, CD366252.1 and BF094375.1. This sequence is a reference standard in the RefSeqGene project. On Feb 18, 2011 this sequence version replaced gi:156071492. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027882.1, X17668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACACG CTATGGAAAA CTCCTGGACA ATCAGTAAAG AGTACCATAT TGATGAAGAA 
GTGGGCTTTG CTCTGCCAAA TCCACAGGAA AATCTACCTG ATTTTTATAA TGACTGGATG
TTCATTGCTA AACATCTGCC TGATCTCATA GAGTCTGGCC AGCTTCGAGA AAGAGTTGAG
AAGTTAAACA TGCTCAGCAT TGATCATCTC ACAGACCACA AGTCACAGCG CCTTGCACGT
CTAGTTCTGG GATGCATCAC CATGGCATAT GTGTGGGGCA AAGGTCATGG AGATGTCCGT
AAGGTCTTGC CAAGAAATAT TGCTGTTCCT TACTGCCAAC TCTCCAAGAA ACTGGAACTG
CCTCCTATTT TGGTTTATGC AGACTGTGTC TTGGCAAACT GGAAGAAAAA GGATCCTAAT
AAGCCCCTGA CTTATGAGAA CATGGACGTT TTGTTCTCAT TTCGTGATGG AGACTGCAGT
AAAGGATTCT TCCTGGTCTC TCTATTGGTG GAAATAGCAG CTGCTTCTGC AATCAAAGTA
ATTCCTACTG TATTCAAGGC AATGCAAATG CAAGAACGGG ACACTTTGCT AAAGGCGCTG
TTGGAAATAG CTTCTTGCTT GGAGAAAGCC CTTCAAGTGT TTCACCAAAT CCACGATCAT
GTGAACCCAA AAGCATTTTT CAGTGTTCTT CGCATATATT TGTCTGGCTG GAAAGGCAAC
CCCCAGCTAT CAGACGGTCT GGTGTATGAA GGGTTCTGGG AAGACCCAAA GGAGTTTGCA
GGGGGCAGTG CAGGCCAAAG CAGCGTCTTT CAGTGCTTTG ACGTCCTGCT GGGCATCCAG
CAGACTGCTG GTGGAGGACA TGCTGCTCAG TTCCTCCAGG ACATGAGAAG ATATATGCCA
CCAGCTCACA GGAACTTCCT GTGCTCATTA GAGTCAAATC CCTCAGTCCG TGAGTTTGTC
CTTTCAAAAG GTGATGCTGG CCTGCGGGAA GCTTATGACG CCTGTGTGAA AGCTCTGGTC
TCCCTGAGGA GCTACCATCT GCAAATCGTG ACTAAGTACA TCCTGATTCC TGCAAGCCAG
CAGCCAAAGG AGAATAAGAC CTCTGAAGAC CCTTCAAAAC TGGAAGCCAA AGGAACTGGA
GGCACTGATT TAATGAATTT CCTGAAGACT GTAAGAAGTA CAACTGAGAA ATCCCTTTTG
AAGGAAGGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002155.1
CDS115..1326
Misc Feature(1)85..87(+)
Misc Feature(2)136..1314(+)
Exon (1)1..201
Gene:IDO1
Gene Synonym:
Exon (2)202..297
Gene:IDO1
Gene Synonym:
Exon (3)298..417
Gene:IDO1
Gene Synonym:
Exon (4)418..536
Gene:IDO1
Gene Synonym:
Exon (5)537..551
Gene:IDO1
Gene Synonym:
Exon (6)552..651
Gene:IDO1
Gene Synonym:
Exon (7)652..769
Gene:IDO1
Gene Synonym:
Exon (8)770..821
Gene:IDO1
Gene Synonym:
Exon (9)822..970
Gene:IDO1
Gene Synonym:
Exon (10)971..1931
Gene:IDO1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002164
Organism Homo sapiens (human)
Definition Homo sapiens indoleamine 2,3-dioxygenase 1 (IDO1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002164

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACACG CTATGGAAAA CTCCTGGACA ATCAGTAAAG AGTACCATAT TGATGAAGAA 
GTGGGCTTTG CTCTGCCAAA TCCACAGGAA AATCTACCTG ATTTTTATAA TGACTGGATG
TTCATTGCTA AACATCTGCC TGATCTCATA GAGTCTGGCC AGCTTCGAGA AAGAGTTGAG
AAGTTAAACA TGCTCAGCAT TGATCATCTC ACAGACCACA AGTCACAGCG CCTTGCACGT
CTAGTTCTGG GATGCATCAC CATGGCATAT GTGTGGGGCA AAGGTCATGG AGATGTCCGT
AAGGTCTTGC CAAGAAATAT TGCTGTTCCT TACTGCCAAC TCTCCAAGAA ACTGGAACTG
CCTCCTATTT TGGTTTATGC AGACTGTGTC TTGGCAAACT GGAAGAAAAA GGATCCTAAT
AAGCCCCTGA CTTATGAGAA CATGGACGTT TTGTTCTCAT TTCGTGATGG AGACTGCAGT
AAAGGATTCT TCCTGGTCTC TCTATTGGTG GAAATAGCAG CTGCTTCTGC AATCAAAGTA
ATTCCTACTG TATTCAAGGC AATGCAAATG CAAGAACGGG ACACTTTGCT AAAGGCGCTG
TTGGAAATAG CTTCTTGCTT GGAGAAAGCC CTTCAAGTGT TTCACCAAAT CCACGATCAT
GTGAACCCAA AAGCATTTTT CAGTGTTCTT CGCATATATT TGTCTGGCTG GAAAGGCAAC
CCCCAGCTAT CAGACGGTCT GGTGTATGAA GGGTTCTGGG AAGACCCAAA GGAGTTTGCA
GGGGGCAGTG CAGGCCAAAG CAGCGTCTTT CAGTGCTTTG ACGTCCTGCT GGGCATCCAG
CAGACTGCTG GTGGAGGACA TGCTGCTCAG TTCCTCCAGG ACATGAGAAG ATATATGCCA
CCAGCTCACA GGAACTTCCT GTGCTCATTA GAGTCAAATC CCTCAGTCCG TGAGTTTGTC
CTTTCAAAAG GTGATGCTGG CCTGCGGGAA GCTTATGACG CCTGTGTGAA AGCTCTGGTC
TCCCTGAGGA GCTACCATCT GCAAATCGTG ACTAAGTACA TCCTGATTCC TGCAAGCCAG
CAGCCAAAGG AGAATAAGAC CTCTGAAGAC CCTTCAAAAC TGGAAGCCAA AGGAACTGGA
GGCACTGATT TAATGAATTT CCTGAAGACT GTAAGAAGTA CAACTGAGAA ATCCCTTTTG
AAGGAAGGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Checks and balances between human cytomegalovirus replication and indoleamine-2,3-dioxygenase
J. Gen. Virol. 95 (PT 3), 659-670 (2014)
Zimmermann A, Hauka S, Maywald M, Le VT, Schmidt SK, Daubener W and Hengel H.


book

The SOCS3-independent expression of IDO2 supports the homeostatic generation of T regulatory cells by human dendritic cells
J. Immunol. 192 (3), 1231-1240 (2014)
Trabanelli S, Ocadlikova D, Ciciarello M, Salvestrini V, Lecciso M, Jandus C, Metz R, Evangelisti C, Laury-Kleintop L, Romero P, Prendergast GC, Curti A and Lemoli RM.


book

Pro-inflammatory cytokines downregulate Hsp27 and cause apoptosis of human retinal capillary endothelial cells
Biochim. Biophys. Acta 1842 (2), 164-174 (2014)
Nahomi RB, Palmer A, Green KM, Fort PE and Nagaraj RH.


book

Cyclooxygenase-2 (COX-2) inhibition constrains indoleamine 2,3-dioxygenase 1 (IDO1) activity in acute myeloid leukaemia cells
Molecules 18 (9), 10132-10145 (2013)
Iachininoto MG, Nuzzolo ER, Bonanno G, Mariotti A, Procoli A, Locatelli F, De Cristofaro R and Rutella S.


book

IL-22 and IDO1 affect immunity and tolerance to murine and human vaginal candidiasis
PLoS Pathog. 9 (7), E1003486 (2013)
De Luca A, Carvalho A, Cunha C, Iannitti RG, Pitzurra L, Giovannini G, Mencacci A, Bartolommei L, Moretti S, Massi-Benedetti C, Fuchs D, De Bernardis F, Puccetti P and Romani L.


book

Gene structure of human indoleamine 2,3-dioxygenase
Biochem. Biophys. Res. Commun. 189 (1), 530-536 (1992)
Kadoya A, Tone S, Maeda H, Minatogawa Y and Kido R.


book

Localization and developmental change of indoleamine 2,3-dioxygenase activity in the human placenta
Acta Med. Okayama 45 (3), 135-139 (1991)
Kamimura S, Eguchi K, Yonezawa M and Sekiba K.


book

Molecular cloning, sequencing and expression of human interferon-gamma-inducible indoleamine 2,3-dioxygenase cDNA
Biochem. Biophys. Res. Commun. 168 (1), 1-8 (1990)
Dai W and Gupta SL.


book

Primary structure of human indoleamine 2,3-dioxygenase deduced from the nucleotide sequence of its cDNA
Nucleic Acids Res. 18 (2), 367 (1990)
Tone S, Takikawa O, Habara-Ohkubo A, Kadoya A, Yoshida R and Kido R.


book

Tumour necrosis factor-alpha and lipopolysaccharide enhance interferon-induced tryptophan degradation and pteridine synthesis in human cells
Biol. Chem. Hoppe-Seyler 370 (9), 1063-1069 (1989)
Werner-Felmayer G, Werner ER, Fuchs D, Hausen A, Reibnegger G and Wachter H.


 
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