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KRT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol KRT1
Entrez Gene ID 3848
Full Name keratin 1, type II
Synonyms CK1, EHK, EHK1, EPPK, K1, KRT1A, NEPPK
General protein information
Preferred Names
keratin, type II cytoskeletal 1
Names
keratin, type II cytoskeletal 1
CK-1
cytokeratin 1
cytokeratin-1
67 kDa cytokeratin
hair alpha protein
type-II keratin Kb1
epidermolytic hyperkeratosis 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13.13

Summary The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

139350

Disorder Html: Epidermolytic hyperkeratosis, 113800 (3); Ichthyosis, cyclic, with

mRNA and Protein(s)

mRNA Protein Name
NM_006121 NP_006112 keratin, type II cytoskeletal 1



Homo sapiens (human) KRT1 NP_006112.3
Pan troglodytes (chimpanzee) KRT1 NP_001104288.1
Macaca mulatta (Rhesus monkey) KRT1 XP_001098082.2
Canis lupus familiaris (dog) KRT1 NP_001003392.1
Bos taurus (cattle) KRT1 XP_002687292.1
Mus musculus (house mouse) Krt1 NP_032499.2
Rattus norvegicus (Norway rat) Krt1 NP_001008802.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19449 NM_006121 Homo sapiens keratin 1, type II (KRT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
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2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19449
Clone ID Related Accession (Same CDS sequence) NM_006121
Accession Version NM_006121.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cytoskeletal 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from DB112126.1, BC063697.1 and AC055716.24. This sequence is a reference standard in the RefSeqGene project. On Dec 14, 2006 this sequence version replaced gi:17318568. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063697.1, M10938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAGTCGAC AGTTTAGTTC CAGGTCTGGG TACCGAAGTG GAGGGGGCTT CAGCTCTGGC 
TCTGCTGGGA TCATCAACTA CCAGCGCAGG ACCACCAGCA GCTCCACACG CCGCAGTGGA
GGAGGTGGTG GGAGATTTTC AAGCTGTGGT GGTGGTGGTG GTAGCTTTGG TGCTGGTGGT
GGATTTGGAA GTCGGAGTCT TGTTAACCTT GGTGGCAGTA AAAGCATCTC CATAAGTGTG
GCTAGAGGAG GTGGACGTGG TAGTGGCTTT GGTGGTGGTT ATGGTGGTGG TGGCTTTGGT
GGTGGTGGCT TTGGTGGTGG TGGCTTTGGT GGAGGTGGCA TTGGGGGTGG TGGCTTTGGT
GGTTTTGGCA GTGGTGGTGG TGGTTTTGGT GGAGGTGGCT TTGGGGGTGG TGGATATGGG
GGTGGTTATG GTCCTGTCTG CCCTCCTGGT GGCATACAAG AAGTCACTAT CAACCAGAGC
CTTCTTCAGC CCCTCAATGT GGAGATTGAC CCTGAGATCC AAAAGGTGAA GTCTCGAGAA
AGGGAGCAAA TCAAGTCACT CAACAACCAA TTTGCCTCCT TCATTGACAA GGTGAGGTTC
CTGGAGCAGC AGAACCAGGT ACTGCAAACA AAATGGGAGC TGCTGCAGCA GGTAGATACC
TCCACTAGAA CCCATAATTT AGAGCCCTAC TTTGAGTCAT TCATCAACAA TCTCCGAAGG
AGAGTGGACC AACTGAAGAG TGATCAATCT CGGTTGGATT CGGAACTGAA GAACATGCAG
GACATGGTGG AGGATTACCG GAACAAGTAT GAGGATGAAA TCAACAAGCG GACAAATGCA
GAGAATGAAT TTGTGACCAT CAAGAAGGAT GTGGATGGTG CTTATATGAC CAAGGTGGAC
CTTCAGGCCA AACTTGACAA CTTGCAGCAG GAAATTGATT TCCTTACAGC ACTCTACCAA
GCAGAGTTGT CTCAGATGCA GACTCAAATC AGTGAAACTA ATGTCATCCT CTCTATGGAC
AACAACCGCA GTCTCGACCT GGACAGCATC ATTGCTGAGG TCAAGGCCCA GTACGAGGAT
ATAGCCCAGA AGAGCAAAGC TGAGGCCGAG TCCTTGTACC AGAGCAAGTA TGAAGAGCTG
CAGATCACTG CTGGCAGACA TGGGGATAGT GTGAGAAATT CAAAGATAGA AATTTCTGAG
CTGAATCGTG TGATCCAGAG ACTTAGATCT GAAATCGACA ATGTCAAGAA GCAGATCTCC
AACTTGCAGC AGTCCATCAG TGATGCAGAG CAGCGTGGCG AGAATGCCCT CAAGGATGCC
AAGAACAAGC TGAATGACCT GGAGGATGCC CTGCAGCAGG CCAAGGAAGA CCTGGCCCGC
CTGCTGCGCG ACTACCAGGA GCTGATGAAC ACCAAGCTGG CCCTGGATCT GGAGATTGCC
ACCTACAGGA CCCTCCTGGA GGGAGAAGAA AGCAGGATGT CTGGAGAATG TGCCCCGAAC
GTGAGTGTGT CTGTGAGCAC AAGCCACACC ACCATCAGTG GAGGTGGCAG CCGAGGAGGT
GGCGGCGGTG GCTACGGCTC TGGAGGTAGC AGCTATGGCT CCGGAGGTGG TAGCTATGGT
TCTGGAGGTG GCGGCGGCGG CGGCCGTGGC AGCTATGGCT CCGGAGGTAG CAGCTACGGC
TCCGGAGGTG GCAGCTATGG CTCTGGAGGT GGCGGCGGCG GCCATGGCAG CTACGGCTCC
GGAAGCAGCA GTGGGGGCTA CAGAGGTGGC TCTGGAGGCG GCGGCGGCGG CAGCTCTGGC
GGCCGGGGCT CTGGCGGCGG GAGCTCTGGA GGCTCCATAG GAGGCCGGGG ATCCAGCTCT
GGGGGTGTCA AGTCCTCTGG TGGCAGTTCC AGCGTGAAGT TTGTTTCTAC CACTTATTCC
GGAGTAACCA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006112.3
CDS60..1994
Misc Feature(1)51..53(+)
Misc Feature(2)63..596(+)
Misc Feature(3)120..122(+)
Misc Feature(4)120..122(+)
Misc Feature(5)255..257(+)
Misc Feature(6)303..305(+)
Misc Feature(7)597..1535(+)
Misc Feature(8)597..1526(+)
Misc Feature(9)597..704(+)
Misc Feature(10)705..761(+)
Misc Feature(11)762..1037(+)
Misc Feature(12)885..887(+)
Misc Feature(13)1038..1109(+)
Misc Feature(14)1089..1091(+)
Misc Feature(15)1110..1526(+)
Misc Feature(16)1248..1463(+)
Misc Feature(17)1356..1358(+)
Misc Feature(18)1527..1991(+)
Exon (1)1..650
Gene:KRT1
Gene Synonym:
Exon (2)651..865
Gene:KRT1
Gene Synonym:
Exon (3)866..926
Gene:KRT1
Gene Synonym:
Exon (4)927..1022
Gene:KRT1
Gene Synonym:
Exon (5)1023..1187
Gene:KRT1
Gene Synonym:
Exon (6)1188..1313
Gene:KRT1
Gene Synonym:
Exon (7)1314..1534
Gene:KRT1
Gene Synonym:
Exon (8)1535..1569
Gene:KRT1
Gene Synonym:
Exon (9)1570..2451
Gene:KRT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006121
Organism Homo sapiens (human)
Definition Homo sapiens keratin 1, type II (KRT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006121

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAGTCGAC AGTTTAGTTC CAGGTCTGGG TACCGAAGTG GAGGGGGCTT CAGCTCTGGC 
TCTGCTGGGA TCATCAACTA CCAGCGCAGG ACCACCAGCA GCTCCACACG CCGCAGTGGA
GGAGGTGGTG GGAGATTTTC AAGCTGTGGT GGTGGTGGTG GTAGCTTTGG TGCTGGTGGT
GGATTTGGAA GTCGGAGTCT TGTTAACCTT GGTGGCAGTA AAAGCATCTC CATAAGTGTG
GCTAGAGGAG GTGGACGTGG TAGTGGCTTT GGTGGTGGTT ATGGTGGTGG TGGCTTTGGT
GGTGGTGGCT TTGGTGGTGG TGGCTTTGGT GGAGGTGGCA TTGGGGGTGG TGGCTTTGGT
GGTTTTGGCA GTGGTGGTGG TGGTTTTGGT GGAGGTGGCT TTGGGGGTGG TGGATATGGG
GGTGGTTATG GTCCTGTCTG CCCTCCTGGT GGCATACAAG AAGTCACTAT CAACCAGAGC
CTTCTTCAGC CCCTCAATGT GGAGATTGAC CCTGAGATCC AAAAGGTGAA GTCTCGAGAA
AGGGAGCAAA TCAAGTCACT CAACAACCAA TTTGCCTCCT TCATTGACAA GGTGAGGTTC
CTGGAGCAGC AGAACCAGGT ACTGCAAACA AAATGGGAGC TGCTGCAGCA GGTAGATACC
TCCACTAGAA CCCATAATTT AGAGCCCTAC TTTGAGTCAT TCATCAACAA TCTCCGAAGG
AGAGTGGACC AACTGAAGAG TGATCAATCT CGGTTGGATT CGGAACTGAA GAACATGCAG
GACATGGTGG AGGATTACCG GAACAAGTAT GAGGATGAAA TCAACAAGCG GACAAATGCA
GAGAATGAAT TTGTGACCAT CAAGAAGGAT GTGGATGGTG CTTATATGAC CAAGGTGGAC
CTTCAGGCCA AACTTGACAA CTTGCAGCAG GAAATTGATT TCCTTACAGC ACTCTACCAA
GCAGAGTTGT CTCAGATGCA GACTCAAATC AGTGAAACTA ATGTCATCCT CTCTATGGAC
AACAACCGCA GTCTCGACCT GGACAGCATC ATTGCTGAGG TCAAGGCCCA GTACGAGGAT
ATAGCCCAGA AGAGCAAAGC TGAGGCCGAG TCCTTGTACC AGAGCAAGTA TGAAGAGCTG
CAGATCACTG CTGGCAGACA TGGGGATAGT GTGAGAAATT CAAAGATAGA AATTTCTGAG
CTGAATCGTG TGATCCAGAG ACTTAGATCT GAAATCGACA ATGTCAAGAA GCAGATCTCC
AACTTGCAGC AGTCCATCAG TGATGCAGAG CAGCGTGGCG AGAATGCCCT CAAGGATGCC
AAGAACAAGC TGAATGACCT GGAGGATGCC CTGCAGCAGG CCAAGGAAGA CCTGGCCCGC
CTGCTGCGCG ACTACCAGGA GCTGATGAAC ACCAAGCTGG CCCTGGATCT GGAGATTGCC
ACCTACAGGA CCCTCCTGGA GGGAGAAGAA AGCAGGATGT CTGGAGAATG TGCCCCGAAC
GTGAGTGTGT CTGTGAGCAC AAGCCACACC ACCATCAGTG GAGGTGGCAG CCGAGGAGGT
GGCGGCGGTG GCTACGGCTC TGGAGGTAGC AGCTATGGCT CCGGAGGTGG TAGCTATGGT
TCTGGAGGTG GCGGCGGCGG CGGCCGTGGC AGCTATGGCT CCGGAGGTAG CAGCTACGGC
TCCGGAGGTG GCAGCTATGG CTCTGGAGGT GGCGGCGGCG GCCATGGCAG CTACGGCTCC
GGAAGCAGCA GTGGGGGCTA CAGAGGTGGC TCTGGAGGCG GCGGCGGCGG CAGCTCTGGC
GGCCGGGGCT CTGGCGGCGG GAGCTCTGGA GGCTCCATAG GAGGCCGGGG ATCCAGCTCT
GGGGGTGTCA AGTCCTCTGG TGGCAGTTCC AGCGTGAAGT TTGTTTCTAC CACTTATTCC
GGAGTAACCA GATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of endothelial cell surface antigens encoded by genes other than HLA. A combined immunoprecipitation and proteomic approach for the identification of antigens recognized by antibodies against endothelial cells in transplant recipients
Hum. Immunol. 74 (11), 1445-1452 (2013)
Qin Z, Zou Y, Lavingia B and Stastny P.


book

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance
J. Biol. Chem. 288 (26), 18716-18731 (2013)
Soteriou D, Iskender B, Byron A, Humphries JD, Borg-Bartolo S, Haddock MC, Baxter MA, Knight D, Humphries MJ and Kimber SJ.


book

Mild phenotype of epidermolytic hyperkeratosis mimicking ichthyosis bullosa of Siemens is related to specific mutation in 2B domain of KRT1
J. Dermatol. Sci. 70 (3), 220-222 (2013)
Sung,J.Y., Oh,S.W., Kim,S.E. and Kim,S.C.


book

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma naive to therapy
Mol Biosyst 9 (6), 1108-1116 (2013)
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A and Del Boccio P.


book

Epidemiology and clinical characteristics of bullous congenital ichthyosiform erythroderma (keratinolytic ichthyosis) in Japan: results from a nationwide survey
J. Am. Acad. Dermatol. 68 (2), 278-283 (2013)
Kurosawa M, Takagi A, Tamakoshi A, Kawamura T, Inaba Y, Yokoyama K, Kitajima Y, Aoyama Y, Iwatsuki K and Ikeda S.


book

The two size alleles of human keratin 1 are due to a deletion in the glycine-rich carboxyl-terminal V2 subdomain
J. Invest. Dermatol. 99 (6), 697-702 (1992)
Korge BP, Compton JG, Steinert PM and Mischke D.


book

Linkage of epidermolytic hyperkeratosis to the type II keratin gene cluster on chromosome 12q
Nat. Genet. 1 (4), 301-305 (1992)
Compton JG, DiGiovanna JJ, Santucci SK, Kearns KS, Amos CI, Abangan DL, Korge BP, McBride OW, Steinert PM and Bale SJ.


book

Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin
J. Infect. 24 (3), 317-320 (1992)
Dawson SJ and White LA.


book

Chromosomal mapping of human keratin genes: evidence of non-linkage
J. Invest. Dermatol. 91 (6), 572-578 (1988)
Lessin SR, Huebner K, Isobe M, Croce CM and Steinert PM.


book

Role of transforming growth factor beta in the maturation of human epidermal keratinocytes
J. Invest. Dermatol. 90 (3), 336-341 (1988)
Mansbridge JN and Hanawalt PC.


 
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