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SPRED3 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SPRED3
Entrez Gene ID 399473
Full Name sprouty related EVH1 domain containing 3
Synonyms Eve-3,spred-3
General protein information
Preferred Names

sprouty related EVH1 domain containing 3

Names

sprouty-related, EVH1 domain-containing protein 3
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

19

19q13.2
Summary This gene encodes a protein with a C-terminal Sprouty-like cysteine-rich domain (SRY) and an N-terminal Ena/Vasodilator-stimulated phosphoprotein (VASP) homology-1 (EVH-1) domain. The encoded protein is a member of a family of proteins that negatively regulates mitogen-activated protein (MAP) kinase signaling, particularly during organogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012].
Disorder MIM:

609293


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011526978.2 XP_011525280.1 sprouty-related, EVH1 domain-containing protein 3 isoform X2
XM_006723219.3 XP_006723282.1 sprouty-related, EVH1 domain-containing protein 3 isoform X1
NM_001042522.2 NP_001035987.1 sprouty-related, EVH1 domain-containing protein 3

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) SPRED3 XP_005616679.1
Pan troglodytes (chimpanzee) SPRED3 XP_524251.3
LOC715133 XP_001107519.1
Bos taurus (cattle) SPRED3 XP_003587312.2
Rattus norvegicus (Norway rat) Spred3 NP_001166815.1
Homo sapiens (human) SPRED3 NP_001035987.1
Mus musculus (house mouse) Spred3 NP_891557.3

Gene Ontology


Bibliography

Related articles in PubMed

Sprouty proteins: modified modulators, matchmakers or missing links?
Guy GR, Jackson RA, Yusoff P, Chow SY
The Journal of endocrinology203(2)191-202(2009 Nov)

Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination.
Lock P, I ST, Straffon AF, Schieb H, Hovens CM, Stylli SS
Biochemical and biophysical research communications351(4)1018-23(2006 Dec)

Eve-3: a liver enriched suppressor of Ras/MAPK signaling.
King JA, Corcoran NM, D'Abaco GM, Straffon AF, Smith CT, Poon CL, Buchert M, I S, Hall NE, Lock P, Hovens CM
Journal of hepatology44(4)758-67(2006 Apr)

FGF signaling inhibitor, SPRY4, is evolutionarily conserved target of WNT signaling pathway in progenitor cells.
Katoh Y, Katoh M
International journal of molecular medicine17(3)529-32(2006 Mar)

Mutation analysis of CBL-C and SPRED3 on 19q in human glioblastoma.
Mizoguchi M, Nutt CL, Louis DN
Neurogenetics5(1)81-2(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPRED3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPRED3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14619 NM_001042522.2
Latest version!		 Homo sapiens sprouty related EVH1 domain containing 3 (SPRED3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu14619 XM_006723219.3
Latest version!		 Homo sapiens sprouty related EVH1 domain containing 3 (SPRED3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu80072 XM_011526978.2
Latest version!		 Homo sapiens sprouty related EVH1 domain containing 3 (SPRED3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu14619
Clone ID Related Accession (Same CDS sequence) XM_006723219.3 , NM_001042522.2
Accession Version NM_001042522.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1233bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-05
Organism Homo sapiens(human)
Product sprouty-related, EVH1 domain-containing protein 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC151136.1, AC005789.1, AI028379.1 and AI871425.1. On Jul 18, 2012 this sequence version replaced NM_001042522.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC151136.1, DQ323928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

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ATGGTGCGGG TCCGAGCTGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTGTGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAAC GCCTCCGGGA CCAGAAAACA 
ACCTTGGAGT GTACACTGAA GCCAGGCTTG GTTTACAACA AGGTGAATCC CATCTTTCAC 
CACTGGAGCC TGGGTGACTG CAAGTTTGGA CTGACGTTTC AGAGCCCTGC AGAGGCTGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCCGCACTGG GTCGAGGCTC ACTCACCCCC 
TCCTCCTCCT CCTCCTCCTC CTCTCCTTCC CAGGATACTG CAGAGACCCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCAGC 
GCCGCTGCGG CCCCCATCAT CACGATGGAG TCAGCTTCAG GCTTCGGGCC GACCACGCCC 
CCCCAGCGCC GCCGCTCCTC CGCTCAGAGC TACCCTCCGC TTCTACCGTT CACGGGGATT 
CCGGAACCCT CAGAGCCCCT GGCAGGGGCA GGGGGCCTGG GGTGGGGCGG CCGCGGCTAC 
GAGGATTACC GGCGCTCCGG GCCACCCGCG CCCCTCGCCC TGTCCACCTG CGTCGTGCGC 
TTCGCCAAGA CCGGCGCGTT GAGGGGCGCT GCCCTGGGTC CCCCAGCGGC ACTACCTGCC 
CCTTTGACCG AGGCTGCGCC CCCAGCGCCC CCCGCTCGCC CACCCCCCGG CCCGGGCCCA 
TCCTCTGCGC CTGCCAAGGC CTCCCCGGAA GCGGAGGAGG CAGCGCGCTG CGTGCATTGC 
CGCGCGCTCT TCCGTCGCAG AGCAGACGGG CGTGGCGGCC GCTGCGCAGA GGCCCCGGAC 
CCGGGTCGCC TCCTGGTGCG CCGTCTAAGC TGCCTGTGGT GCGCCGAGAG CTTGCTCTAC 
CACTGCCTGT CGGACGCCGA GGGCGACTTC TCGGACCCGT GCGCCTGCGA GCCGGGCCAC 
CCGCGCCCCG CCGCGCGCTG GGCCGCGCTG GCCGCGCTCT CCCTGGCAGT GCCCTGCCTC 
TGCTGCTACG CGCCCCTGCG CGCGTGCCAC TGGGTCGCAG CGCGATGCGG CTGCGCCGGC 
TGCGGGGGTC GCCACGAGGA GGCTGCGCGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001035987.1
CDS104..1336
Translation

Target ORF information:

RefSeq Version NM_001042522.2
Organism Homo sapiens(human)
Definition Homo sapiens sprouty related EVH1 domain containing 3 (SPRED3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001042522.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCTGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTGTGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAAC GCCTCCGGGA CCAGAAAACA 
ACCTTGGAGT GTACACTGAA GCCAGGCTTG GTTTACAACA AGGTGAATCC CATCTTTCAC 
CACTGGAGCC TGGGTGACTG CAAGTTTGGA CTGACGTTTC AGAGCCCTGC AGAGGCTGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCCGCACTGG GTCGAGGCTC ACTCACCCCC 
TCCTCCTCCT CCTCCTCCTC CTCTCCTTCC CAGGATACTG CAGAGACCCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCAGC 
GCCGCTGCGG CCCCCATCAT CACGATGGAG TCAGCTTCAG GCTTCGGGCC GACCACGCCC 
CCCCAGCGCC GCCGCTCCTC CGCTCAGAGC TACCCTCCGC TTCTACCGTT CACGGGGATT 
CCGGAACCCT CAGAGCCCCT GGCAGGGGCA GGGGGCCTGG GGTGGGGCGG CCGCGGCTAC 
GAGGATTACC GGCGCTCCGG GCCACCCGCG CCCCTCGCCC TGTCCACCTG CGTCGTGCGC 
TTCGCCAAGA CCGGCGCGTT GAGGGGCGCT GCCCTGGGTC CCCCAGCGGC ACTACCTGCC 
CCTTTGACCG AGGCTGCGCC CCCAGCGCCC CCCGCTCGCC CACCCCCCGG CCCGGGCCCA 
TCCTCTGCGC CTGCCAAGGC CTCCCCGGAA GCGGAGGAGG CAGCGCGCTG CGTGCATTGC 
CGCGCGCTCT TCCGTCGCAG AGCAGACGGG CGTGGCGGCC GCTGCGCAGA GGCCCCGGAC 
CCGGGTCGCC TCCTGGTGCG CCGTCTAAGC TGCCTGTGGT GCGCCGAGAG CTTGCTCTAC 
CACTGCCTGT CGGACGCCGA GGGCGACTTC TCGGACCCGT GCGCCTGCGA GCCGGGCCAC 
CCGCGCCCCG CCGCGCGCTG GGCCGCGCTG GCCGCGCTCT CCCTGGCAGT GCCCTGCCTC 
TGCTGCTACG CGCCCCTGCG CGCGTGCCAC TGGGTCGCAG CGCGATGCGG CTGCGCCGGC 
TGCGGGGGTC GCCACGAGGA GGCTGCGCGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14619
Clone ID Related Accession (Same CDS sequence) XM_006723219.3 , NM_001042522.2
Accession Version XM_006723219.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1233bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCTGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTGTGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAAC GCCTCCGGGA CCAGAAAACA 
ACCTTGGAGT GTACACTGAA GCCAGGCTTG GTTTACAACA AGGTGAATCC CATCTTTCAC 
CACTGGAGCC TGGGTGACTG CAAGTTTGGA CTGACGTTTC AGAGCCCTGC AGAGGCTGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCCGCACTGG GTCGAGGCTC ACTCACCCCC 
TCCTCCTCCT CCTCCTCCTC CTCTCCTTCC CAGGATACTG CAGAGACCCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCAGC 
GCCGCTGCGG CCCCCATCAT CACGATGGAG TCAGCTTCAG GCTTCGGGCC GACCACGCCC 
CCCCAGCGCC GCCGCTCCTC CGCTCAGAGC TACCCTCCGC TTCTACCGTT CACGGGGATT 
CCGGAACCCT CAGAGCCCCT GGCAGGGGCA GGGGGCCTGG GGTGGGGCGG CCGCGGCTAC 
GAGGATTACC GGCGCTCCGG GCCACCCGCG CCCCTCGCCC TGTCCACCTG CGTCGTGCGC 
TTCGCCAAGA CCGGCGCGTT GAGGGGCGCT GCCCTGGGTC CCCCAGCGGC ACTACCTGCC 
CCTTTGACCG AGGCTGCGCC CCCAGCGCCC CCCGCTCGCC CACCCCCCGG CCCGGGCCCA 
TCCTCTGCGC CTGCCAAGGC CTCCCCGGAA GCGGAGGAGG CAGCGCGCTG CGTGCATTGC 
CGCGCGCTCT TCCGTCGCAG AGCAGACGGG CGTGGCGGCC GCTGCGCAGA GGCCCCGGAC 
CCGGGTCGCC TCCTGGTGCG CCGTCTAAGC TGCCTGTGGT GCGCCGAGAG CTTGCTCTAC 
CACTGCCTGT CGGACGCCGA GGGCGACTTC TCGGACCCGT GCGCCTGCGA GCCGGGCCAC 
CCGCGCCCCG CCGCGCGCTG GGCCGCGCTG GCCGCGCTCT CCCTGGCAGT GCCCTGCCTC 
TGCTGCTACG CGCCCCTGCG CGCGTGCCAC TGGGTCGCAG CGCGATGCGG CTGCGCCGGC 
TGCGGGGGTC GCCACGAGGA GGCTGCGCGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723282.1
CDS95..1327
Translation

Target ORF information:

RefSeq Version XM_006723219.3
Organism Homo sapiens(human)
Definition Homo sapiens sprouty related EVH1 domain containing 3 (SPRED3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006723219.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTGCGGG TCCGAGCTGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGGTGAGC GTGTGTCGGG TCCGAGGGGC CAGGCCCGAG 
GGGGGGGCCC GCCAGGGGCA CTACGTCATC CACGGGGAAC GCCTCCGGGA CCAGAAAACA 
ACCTTGGAGT GTACACTGAA GCCAGGCTTG GTTTACAACA AGGTGAATCC CATCTTTCAC 
CACTGGAGCC TGGGTGACTG CAAGTTTGGA CTGACGTTTC AGAGCCCTGC AGAGGCTGAT 
GAGTTCCAGA AGAGCCTGCT GGCTGCGCTG GCCGCACTGG GTCGAGGCTC ACTCACCCCC 
TCCTCCTCCT CCTCCTCCTC CTCTCCTTCC CAGGATACTG CAGAGACCCC CTGCCCTCTG 
ACGTCCCACG TGGACAGCGA CTCCTCCTCC AGTCACAGCC GCCAGGAGAC TCCTCCCAGC 
GCCGCTGCGG CCCCCATCAT CACGATGGAG TCAGCTTCAG GCTTCGGGCC GACCACGCCC 
CCCCAGCGCC GCCGCTCCTC CGCTCAGAGC TACCCTCCGC TTCTACCGTT CACGGGGATT 
CCGGAACCCT CAGAGCCCCT GGCAGGGGCA GGGGGCCTGG GGTGGGGCGG CCGCGGCTAC 
GAGGATTACC GGCGCTCCGG GCCACCCGCG CCCCTCGCCC TGTCCACCTG CGTCGTGCGC 
TTCGCCAAGA CCGGCGCGTT GAGGGGCGCT GCCCTGGGTC CCCCAGCGGC ACTACCTGCC 
CCTTTGACCG AGGCTGCGCC CCCAGCGCCC CCCGCTCGCC CACCCCCCGG CCCGGGCCCA 
TCCTCTGCGC CTGCCAAGGC CTCCCCGGAA GCGGAGGAGG CAGCGCGCTG CGTGCATTGC 
CGCGCGCTCT TCCGTCGCAG AGCAGACGGG CGTGGCGGCC GCTGCGCAGA GGCCCCGGAC 
CCGGGTCGCC TCCTGGTGCG CCGTCTAAGC TGCCTGTGGT GCGCCGAGAG CTTGCTCTAC 
CACTGCCTGT CGGACGCCGA GGGCGACTTC TCGGACCCGT GCGCCTGCGA GCCGGGCCAC 
CCGCGCCCCG CCGCGCGCTG GGCCGCGCTG GCCGCGCTCT CCCTGGCAGT GCCCTGCCTC 
TGCTGCTACG CGCCCCTGCG CGCGTGCCAC TGGGTCGCAG CGCGATGCGG CTGCGCCGGC 
TGCGGGGGTC GCCACGAGGA GGCTGCGCGG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu80072
Clone ID Related Accession (Same CDS sequence) XM_011526978.2
Accession Version XM_011526978.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1140bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product sprouty-related, EVH1 domain-containing protein 3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGTGCGGG TCCGAGCTGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGACAACC TTGGAGTGTA CACTGAAGCC AGGCTTGGTT 
TACAACAAGG TGAATCCCAT CTTTCACCAC TGGAGCCTGG GTGACTGCAA GTTTGGACTG 
ACGTTTCAGA GCCCTGCAGA GGCTGATGAG TTCCAGAAGA GCCTGCTGGC TGCGCTGGCC 
GCACTGGGTC GAGGCTCACT CACCCCCTCC TCCTCCTCCT CCTCCTCCTC TCCTTCCCAG 
GATACTGCAG AGACCCCCTG CCCTCTGACG TCCCACGTGG ACAGCGACTC CTCCTCCAGT 
CACAGCCGCC AGGAGACTCC TCCCAGCGCC GCTGCGGCCC CCATCATCAC GATGGAGTCA 
GCTTCAGGCT TCGGGCCGAC CACGCCCCCC CAGCGCCGCC GCTCCTCCGC TCAGAGCTAC 
CCTCCGCTTC TACCGTTCAC GGGGATTCCG GAACCCTCAG AGCCCCTGGC AGGGGCAGGG 
GGCCTGGGGT GGGGCGGCCG CGGCTACGAG GATTACCGGC GCTCCGGGCC ACCCGCGCCC 
CTCGCCCTGT CCACCTGCGT CGTGCGCTTC GCCAAGACCG GCGCGTTGAG GGGCGCTGCC 
CTGGGTCCCC CAGCGGCACT ACCTGCCCCT TTGACCGAGG CTGCGCCCCC AGCGCCCCCC 
GCTCGCCCAC CCCCCGGCCC GGGCCCATCC TCTGCGCCTG CCAAGGCCTC CCCGGAAGCG 
GAGGAGGCAG CGCGCTGCGT GCATTGCCGC GCGCTCTTCC GTCGCAGAGC AGACGGGCGT 
GGCGGCCGCT GCGCAGAGGC CCCGGACCCG GGTCGCCTCC TGGTGCGCCG TCTAAGCTGC 
CTGTGGTGCG CCGAGAGCTT GCTCTACCAC TGCCTGTCGG ACGCCGAGGG CGACTTCTCG 
GACCCGTGCG CCTGCGAGCC GGGCCACCCG CGCCCCGCCG CGCGCTGGGC CGCGCTGGCC 
GCGCTCTCCC TGGCAGTGCC CTGCCTCTGC TGCTACGCGC CCCTGCGCGC GTGCCACTGG 
GTCGCAGCGC GATGCGGCTG CGCCGGCTGC GGGGGTCGCC ACGAGGAGGC TGCGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525280.1
CDS243..1382
Translation

Target ORF information:

RefSeq Version XM_011526978.2
Organism Homo sapiens(human)
Definition Homo sapiens sprouty related EVH1 domain containing 3 (SPRED3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011526978.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGTGCGGG TCCGAGCTGT GGTGATGGCC CGAGATGACT CCAGTGGGGG CTGGCTGCCT 
GTGGGGGGCG GGGGCCTCAG CCAGACAACC TTGGAGTGTA CACTGAAGCC AGGCTTGGTT 
TACAACAAGG TGAATCCCAT CTTTCACCAC TGGAGCCTGG GTGACTGCAA GTTTGGACTG 
ACGTTTCAGA GCCCTGCAGA GGCTGATGAG TTCCAGAAGA GCCTGCTGGC TGCGCTGGCC 
GCACTGGGTC GAGGCTCACT CACCCCCTCC TCCTCCTCCT CCTCCTCCTC TCCTTCCCAG 
GATACTGCAG AGACCCCCTG CCCTCTGACG TCCCACGTGG ACAGCGACTC CTCCTCCAGT 
CACAGCCGCC AGGAGACTCC TCCCAGCGCC GCTGCGGCCC CCATCATCAC GATGGAGTCA 
GCTTCAGGCT TCGGGCCGAC CACGCCCCCC CAGCGCCGCC GCTCCTCCGC TCAGAGCTAC 
CCTCCGCTTC TACCGTTCAC GGGGATTCCG GAACCCTCAG AGCCCCTGGC AGGGGCAGGG 
GGCCTGGGGT GGGGCGGCCG CGGCTACGAG GATTACCGGC GCTCCGGGCC ACCCGCGCCC 
CTCGCCCTGT CCACCTGCGT CGTGCGCTTC GCCAAGACCG GCGCGTTGAG GGGCGCTGCC 
CTGGGTCCCC CAGCGGCACT ACCTGCCCCT TTGACCGAGG CTGCGCCCCC AGCGCCCCCC 
GCTCGCCCAC CCCCCGGCCC GGGCCCATCC TCTGCGCCTG CCAAGGCCTC CCCGGAAGCG 
GAGGAGGCAG CGCGCTGCGT GCATTGCCGC GCGCTCTTCC GTCGCAGAGC AGACGGGCGT 
GGCGGCCGCT GCGCAGAGGC CCCGGACCCG GGTCGCCTCC TGGTGCGCCG TCTAAGCTGC 
CTGTGGTGCG CCGAGAGCTT GCTCTACCAC TGCCTGTCGG ACGCCGAGGG CGACTTCTCG 
GACCCGTGCG CCTGCGAGCC GGGCCACCCG CGCCCCGCCG CGCGCTGGGC CGCGCTGGCC 
GCGCTCTCCC TGGCAGTGCC CTGCCTCTGC TGCTACGCGC CCCTGCGCGC GTGCCACTGG 
GTCGCAGCGC GATGCGGCTG CGCCGGCTGC GGGGGTCGCC ACGAGGAGGC TGCGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Sprouty proteins: modified modulators, matchmakers or missing links?
The Journal of endocrinology203(2)191-202(2009 Nov)
Guy GR,Jackson RA,Yusoff P,Chow SY


book

Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination.
Biochemical and biophysical research communications351(4)1018-23(2006 Dec)
Lock P,I ST,Straffon AF,Schieb H,Hovens CM,Stylli SS


book

Eve-3: a liver enriched suppressor of Ras/MAPK signaling.
Journal of hepatology44(4)758-67(2006 Apr)
King JA,Corcoran NM,D'Abaco GM,Straffon AF,Smith CT,Poon CL,Buchert M,I S,Hall NE,Lock P,Hovens CM


book

FGF signaling inhibitor, SPRY4, is evolutionarily conserved target of WNT signaling pathway in progenitor cells.
International journal of molecular medicine17(3)529-32(2006 Mar)
Katoh Y,Katoh M


book

Mutation analysis of CBL-C and SPRED3 on 19q in human glioblastoma.
Neurogenetics5(1)81-2(2004 Feb)
Mizoguchi M,Nutt CL,Louis DN