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GTF2H5 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol GTF2H5
Entrez Gene ID 404672
Full Name general transcription factor IIH subunit 5
Synonyms C6orf175,TFB5,TFIIH,TGF2H5,TTD,TTD-A,TTD3,TTDA,bA120J8.2
General protein information
Preferred Names

general transcription factor IIH subunit 5

Names

general transcription factor IIH subunit 5
TFB5 ortholog
TFIIH basal transcription factor complex TTD-A subunit
TFIIH basal transcription factor complex TTDA subunit
general transcription factor IIH, polypeptide 5

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

6

6q25.3

Summary This gene encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating cellular levels of TFIIH. Mutations in this gene result in trichothiodystrophy, complementation group A. [provided by RefSeq, Mar 2009].
Disorder MIM:

608780

Disorder Html: Trichothiodystrophy, complementation group A, 601675 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_207118.2 NP_997001.1 general transcription factor IIH subunit 5
XM_017010862.1 XP_016866351.1 general transcription factor IIH subunit 5 isoform X1



Canis lupus familiaris (dog) GTF2H5 XP_005615600.1
Danio rerio (zebrafish) gtf2h5 NP_001127834.2
Homo sapiens (human) GTF2H5 NP_997001.1
Pan troglodytes (chimpanzee) GTF2H5 XP_001145020.1
Bos taurus (cattle) GTF2H5 NP_001035627.1
Mus musculus (house mouse) Gtf2h5 NP_852057.2
Xenopus tropicalis (tropical clawed frog) gtf2h5 NP_001017298.1
Gallus gallus (chicken) GTF2H5 NP_001091004.1
Rattus norvegicus (Norway rat) Gtf2h5 NP_001119560.1
Drosophila melanogaster (fruit fly) Tfb5 NP_001137791.2
Macaca mulatta (rhesus monkey) GTF2H5 XP_001092693.1


Related articles in PubMed

Near-atomic resolution visualization of human transcription promoter opening.
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature533(7603)359-65(2016 05)

The NER-related gene GTF2H5 predicts survival in high-grade serous ovarian cancer patients.
Gayarre J, Kamieniak MM, Cazorla-Jiménez A, Muñoz-Repeto I, Borrego S, García-Donas J, Hernando S, Robles-Díaz L, García-Bueno JM, Ramón Y Cajal T, Hernández-Agudo E, Heredia Soto V, Márquez-Rodas I, Echarri MJ, Lacambra-Calvet C, Sáez R, Cusidó M, Redondo A, Paz-Ares L, Hardisson D, Mendiola M, Palacios J, Benítez J, García MJ
Journal of gynecologic oncology27(1)e7(2016 Jan)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription.
Singh A, Compe E, Le May N, Egly JM
American journal of human genetics96(2)194-207(2015 Feb)

Genome-wide association study of smoking behaviours among Bangladeshi adults.
Argos M, Tong L, Pierce BL, Rakibuz-Zaman M, Ahmed A, Islam T, Rahman M, Paul-Brutus R, Rahaman R, Roy S, Jasmine F, Kibriya MG, Ahsan H
Journal of medical genetics51(5)327-33(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low levels of GTF2H5 are associated with enhanced prognosis in high-grade serous ovarian cancer patients and may contribute to cisplatin sensitization.
Title: The NER-related gene GTF2H5 predicts survival in high-grade serous ovarian cancer patients.

Transcriptional differences found between various TFIIH subunit variants participate in the phenotypic variability observed among xeroderma pigmentosum, XP associated with Cockayne syndrome, and trichothiodystrophy individuals.
Title: TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription.

Findings give new insights into the behavior of TTDA within the context of a living cell and thereby shed light on the complex phenotype of TTD-A patients.
Title: In vivo interactions of TTDA mutant proteins within TFIIH.

Observational study of gene-disease association. (HuGE Navigator)
Title: Variation within DNA repair pathway genes and risk of multiple sclerosis.

Observational study and meta-analysis of gene-disease association. (HuGE Navigator)
Title: Comprehensive analysis of DNA repair gene variants and risk of meningioma.

The following GTF2H5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF2H5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22965 NM_207118.2
Latest version!
Homo sapiens general transcription factor IIH subunit 5 (GTF2H5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu96602 XM_017010862.1
Latest version!
Homo sapiens general transcription factor IIH subunit 5 (GTF2H5), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00

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Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22965
Clone ID Related Accession (Same CDS sequence) NM_207118.2
Accession Version NM_207118.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 216bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-17
Organism Homo sapiens(Human)
Product general transcription factor IIH subunit 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN403888.1, BC056906.1, AL590703.15, AL133347.28 and BQ004444.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2009 this sequence version replaced NM_207118.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN403888.1, AK055106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGGTCAACG TCTTGAAAGG AGTGCTTATA GAATGTGATC CTGCCATGAA GCAGTTTCTG 
CTGTACTTGG ATGAGTCCAA TGCCCTGGGG AAGAAGTTCA TCATTCAAGA CATTGATGAC
ACTCACGTCT TTGTAATAGC AGAATTGGTT AATGTCCTCC AGGAGCGAGT GGGTGAATTA
ATGGACCAAA ATGCTTTTTC CCTTACCCAG AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997001.1
CDS84..299
Misc Feature(1)75..77
Misc Feature(2)84..287
Misc Feature(3)288..290
Misc Feature(4)288..290
Exon (1)1..49
Exon (2)50..118
Exon (3)119..7486
Translation

Target ORF information:

RefSeq Version NM_207118.2
Organism Homo sapiens(Human)
Definition Homo sapiens general transcription factor IIH subunit 5 (GTF2H5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207118.2

ORF Insert Sequence:

1
61
121
181
ATGGTCAACG TCTTGAAAGG AGTGCTTATA GAATGTGATC CTGCCATGAA GCAGTTTCTG 
CTGTACTTGG ATGAGTCCAA TGCCCTGGGG AAGAAGTTCA TCATTCAAGA CATTGATGAC
ACTCACGTCT TTGTAATAGC AGAATTGGTT AATGTCCTCC AGGAGCGAGT GGGTGAATTA
ATGGACCAAA ATGCTTTTTC CCTTACCCAG AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu96602
Clone ID Related Accession (Same CDS sequence) XM_017010862.1
Accession Version XM_017010862.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 246bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product general transcription factor IIH subunit 5 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
ATGGCGTATC GGCGCAGGCG CACTCTGCCG GCAACGCCGA GGCGCTTCTG CATCTGTGGG 
CCGAGTGATC CTGCCATGAA GCAGTTTCTG CTGTACTTGG ATGAGTCCAA TGCCCTGGGG
AAGAAGTTCA TCATTCAAGA CATTGATGAC ACTCACGTCT TTGTAATAGC AGAATTGGTT
AATGTCCTCC AGGAGCGAGT GGGTGAATTA ATGGACCAAA ATGCTTTTTC CCTTACCCAG
AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016866351.1
CDS16..261
Translation

Target ORF information:

RefSeq Version XM_017010862.1
Organism Homo sapiens(Human)
Definition Homo sapiens general transcription factor IIH subunit 5 (GTF2H5), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017010862.1

ORF Insert Sequence:

1
61
121
181
241
ATGGCGTATC GGCGCAGGCG CACTCTGCCG GCAACGCCGA GGCGCTTCTG CATCTGTGGG 
CCGAGTGATC CTGCCATGAA GCAGTTTCTG CTGTACTTGG ATGAGTCCAA TGCCCTGGGG
AAGAAGTTCA TCATTCAAGA CATTGATGAC ACTCACGTCT TTGTAATAGC AGAATTGGTT
AATGTCCTCC AGGAGCGAGT GGGTGAATTA ATGGACCAAA ATGCTTTTTC CCTTACCCAG
AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Near-atomic resolution visualization of human transcription promoter opening.
Nature533(7603)359-65(2016 05)
He Y,Yan C,Fang J,Inouye C,Tjian R,Ivanov I,Nogales E


book

The NER-related gene GTF2H5 predicts survival in high-grade serous ovarian cancer patients.
Journal of gynecologic oncology27(1)e7(2016 Jan)
Gayarre J,Kamieniak MM,Cazorla-Jiménez A,Muñoz-Repeto I,Borrego S,García-Donas J,Hernando S,Robles-Díaz L,García-Bueno JM,Ramón Y Cajal T,Hernández-Agudo E,Heredia Soto V,Márquez-Rodas I,Echarri MJ,Lacambra-Calvet C,Sáez R,Cusidó M,Redondo A,Paz-Ares L,Hardisson D,Mendiola M,Palacios J,Benítez J,García MJ


book

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M


book

TFIIH subunit alterations causing xeroderma pigmentosum and trichothiodystrophy specifically disturb several steps during transcription.
American journal of human genetics96(2)194-207(2015 Feb)
Singh A,Compe E,Le May N,Egly JM


book

Genome-wide association study of smoking behaviours among Bangladeshi adults.
Journal of medical genetics51(5)327-33(2014 May)
Argos M,Tong L,Pierce BL,Rakibuz-Zaman M,Ahmed A,Islam T,Rahman M,Paul-Brutus R,Rahaman R,Roy S,Jasmine F,Kibriya MG,Ahsan H


 
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