MAP3K1 cDNA ORF clone, Homo sapiens(human)

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu95890 XM_017009485.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $727.30
$1039.00
OHu95889 XM_017009484.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu24943 NM_005921.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
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OHu24943 NM_005921.1 Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu95890
    Clone ID Related Accession (Same CDS sequence) XM_017009485.1
    Accession Version XM_017009485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAAGGGTTG CACAAGATGG ATGATCGTCC AGAGGAACGA 
    ATGATCAGGG AGAAACTGAA GGCAACCTGT ATGCCAGCCT GGAAGCACGA ATGGTTGGAA
    AGGAGAAATA GGCGAGGGCC TGTGGTGGTA AAACCAATCC CAGTTAAAGG AGATGGATCT
    GAAATGAATC ACTTAGCAGC TGAGTCTCCA GGAGAGGTCC AGGCAAGTGC GGCTTCACCA
    GCTTCCAAAG GCCGACGCAG TCCTTCTCCT GGCAACTCCC CATCAGGTCG CACAGTGAAA
    TCAGAATCTC CAGGAGTAAG GAGAAAAAGA GTTTCCCCAG TGCCTTTTCA GAGTGGCAGA
    ATCACACCAC CCCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCTGAGGAA
    ACAAACCGCC GTGTTAACAA AGTGATGCGG GCCAGACTGT ACTTACTGCA GCAGATAGGG
    CCTAACTCTT TCCTGATTGG AGGAGACAGC CCAGACAATA AATACCGGGT GTTTATTGGG
    CCTCAGAACT GCAGCTGTGC ACGTGGAACA TTCTGTATTC ATCTGCTATT TGTGATGCTC
    CGGGTGTTTC AACTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA
    TCTAGTACTT CTACGTCTAG TTCAGAAAAC AGCATAAAGG ATGAAGAGGA ACAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC
    AGGAACAAGC TGCACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGTAG AAGAAATAGA
    GAACCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CTCATGATTT CTACAGCCAC
    GAGTTGTCAA GTCCTGTGGA TTCCCCTTCT TCCCTCAGAG CTGCACAGCA GCAAACCGTA
    CAGCAGCAGC CTTTGGCTGG ATCACGAAGG AATCAAGAGA GCAATTTTAA CCTTACTCAT
    TATGGAACTC AGCAAATCCC TCCTGCTTAC AAAGATTTAG CTGAGCCATG GATTCAGGTG
    TTTGGAATGG AACTCGTTGG CTGCTTATTT TCTAGAAACT GGAATGTGAG AGAGATGGCC
    CTCAGGCGTC TTTCCCATGA TGTCAGTGGG GCCCTGCTGT TGGCAAATGG GGAGAGCACT
    GGAAATTCTG GGGGCAGCAG TGGAAGCAGC CCGAGTGGGG GAGCCACCAG TGGGTCTTCC
    CAGACCAGTA TCTCAGGAGA TGTGGTGGAG GCATGCTGCA GCGTTCTGTC AATGGTCTGT
    GCTGACCCTG TCTACAAAGT GTACGTTGCT GCTTTAAAAA CATTGAGAGC CATGCTGGTA
    TATACTCCTT GCCACAGTTT AGCGGAAAGA ATCAAACTTC AGAGACTTCT CCAGCCAGTT
    GTAGACACCA TCCTAGTCAA ATGTGCAGAT GCCAATAGCC GCACAAGTCA GCTGTCCATA
    TCAACACTGT TGGAACTGTG CAAAGGCCAA GCAGGAGAGT TGGCAGTTGG CAGAGAAATA
    CTAAAAGCTG GATCCATTGG TATTGGTGGT GTTGATTATG TCTTAAATTG TATTCTTGGA
    AACCAAACTG AATCAAACAA TTGGCAAGAA CTTCTTGGCC GCCTTTGTCT TATAGATAGA
    CTGTTGTTGG AATTTCCTGC TGAATTTTAT CCTCATATTG TCAGTACTGA TGTTTCACAA
    GCTGAGCCTG TTGAAATCAG GTATAAGAAG CTGCTGTCCC TCTTAACCTT TGCTTTGCAG
    TCCATTGATA ATTCCCACTC AATGGTTGGC AAACTTTCCA GAAGGATCTA CTTGAGTTCT
    GCAAGAATGG TTACTACAGT ACCCCATGTG TTTTCAAAAC TGTTAGAAAT GCTGAGTGTT
    TCCAGTTCCA CTCACTTCAC CAGGATGCGT CGCCGTTTGA TGGCTATTGC AGATGAGGTG
    GAAATTGCCG AAGCCATCCA GTTGGGCGTA GAAGACACTT TGGATGGTCA ACAGGACAGC
    TTCTTGCAGG CATCTGTTCC CAACAACTAT CTGGAAACCA CAGAGAACAG TTCCCCTGAG
    TGCACAGTCC ATTTAGAGAA AACTGGAAAA GGATTATGTG CTACAAAATT GAGTGCCAGT
    TCAGAGGACA TTTCTGAGAG ACTGGCCAGC ATTTCAGTAG GACCTTCTAG TTCAACAACA
    ACAACAACAA CAACAACAGA GCAACCAAAG CCAATGGTTC AAACAAAAGG CAGACCCCAC
    AGTCAGTGTT TGAACTCCTC TCCTTTATCT CATCATTCCC AATTAATGTT TCCAGCCTTG
    TCAACCCCTT CTTCTTCTAC CCCATCTGTA CCAGCTGGCA CTGCAACAGA TGTCTCTAAG
    CATAGACTTC AGGGATTCAT TCCCTGCAGA ATACCTTCTG CATCTCCTCA AACACAGCGC
    AAGTTTTCTC TACAATTCCA CAGAAACTGT CCTGAAAACA AAGACTCAGA TAAACTTTCC
    CCAGTCTTTA CTCAGTCAAG ACCCTTGCCC TCCAGTAACA TACACAGGCC AAAGCCATCT
    AGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATCCCT CAAAAAATAG CATGACACTT
    GATCTGAACA GTAGTTCCAA ATGTGATGAC AGCTTTGGCT GTAGCAGCAA TAGTAGTAAT
    GCTGTTATAC CCAGTGACGA GACAGTGTTC ACCCCAGTAG AGGAGAAATG CAGATTAGAT
    GTCAATACAG AGCTCAACTC CAGTATTGAG GACCTTCTTG AAGCATCTAT GCCTTCAAGT
    GATACAACAG TAACTTTTAA GTCAGAAGTT GCTGTCCTGT CTCCTGAAAA GGCTGAAAAT
    GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAGAA GATGGAAGCT
    GAAGAAGAAG AAGCTTTAGC AATTGCCATG GCAATGTCAG CGTCTCAGGA TGCCCTCCCC
    ATAGTTCCTC AGCTGCAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA
    CCAGAGACTC TACCAGGACA TACCAAAGCA AAACAACCGT ATAGAGAAGA CACTGAATGG
    CTGAAAGGTC AACAGATAGG CCTTGGAGCA TTTTCTTCTT GTTATCAGGC TCAAGATGTG
    GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACTTATG TCAGAAACAC ATCTTCTGAG
    CAAGAAGAAG TAGTAGAAGC ACTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT
    CCAAACATCA TTAGGATGTT GGGAGCCACG TGTGAGAAGA GCAATTACAA TCTCTTCATT
    GAATGGATGG CAGGGGGATC GGTGGCTCAT TTGCTGAGTA AATATGGAGC CTTCAAAGAA
    TCAGTAGTTA TTAACTACAC TGAACAGTTA CTCCGTGGCC TTTCGTATCT CCATGAAAAC
    CAAATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC TGGTCAGAGA
    CTAAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA
    GAGTTTCAGG GACAATTACT GGGGACAATT GCATTTATGG CACCTGAGGT ACTAAGAGGT
    CAACAGTATG GAAGGAGCTG TGATGTATGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT
    TGTGCAAAAC CACCATGGAA TGCAGAAAAA CACTCCAATC ATCTTGCTTT GATATTTAAG
    ATTGCTAGTG CAACTACTGC TCCATCGATC CCTTCACATT TGTCTCCTGG TTTACGAGAT
    GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCATCAAG AGAGCTACTG
    AAGCATCCAG TCTTTCGTAC TACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864974.1
    CDS430..4479
    Translation

    Target ORF information:

    RefSeq Version XM_017009485.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAAGGGTTG CACAAGATGG ATGATCGTCC AGAGGAACGA 
    ATGATCAGGG AGAAACTGAA GGCAACCTGT ATGCCAGCCT GGAAGCACGA ATGGTTGGAA
    AGGAGAAATA GGCGAGGGCC TGTGGTGGTA AAACCAATCC CAGTTAAAGG AGATGGATCT
    GAAATGAATC ACTTAGCAGC TGAGTCTCCA GGAGAGGTCC AGGCAAGTGC GGCTTCACCA
    GCTTCCAAAG GCCGACGCAG TCCTTCTCCT GGCAACTCCC CATCAGGTCG CACAGTGAAA
    TCAGAATCTC CAGGAGTAAG GAGAAAAAGA GTTTCCCCAG TGCCTTTTCA GAGTGGCAGA
    ATCACACCAC CCCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCTGAGGAA
    ACAAACCGCC GTGTTAACAA AGTGATGCGG GCCAGACTGT ACTTACTGCA GCAGATAGGG
    CCTAACTCTT TCCTGATTGG AGGAGACAGC CCAGACAATA AATACCGGGT GTTTATTGGG
    CCTCAGAACT GCAGCTGTGC ACGTGGAACA TTCTGTATTC ATCTGCTATT TGTGATGCTC
    CGGGTGTTTC AACTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA
    TCTAGTACTT CTACGTCTAG TTCAGAAAAC AGCATAAAGG ATGAAGAGGA ACAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC
    AGGAACAAGC TGCACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGTAG AAGAAATAGA
    GAACCTTTAA TATGTCCCCT TTGTAGATCT AAGTGGAGAT CTCATGATTT CTACAGCCAC
    GAGTTGTCAA GTCCTGTGGA TTCCCCTTCT TCCCTCAGAG CTGCACAGCA GCAAACCGTA
    CAGCAGCAGC CTTTGGCTGG ATCACGAAGG AATCAAGAGA GCAATTTTAA CCTTACTCAT
    TATGGAACTC AGCAAATCCC TCCTGCTTAC AAAGATTTAG CTGAGCCATG GATTCAGGTG
    TTTGGAATGG AACTCGTTGG CTGCTTATTT TCTAGAAACT GGAATGTGAG AGAGATGGCC
    CTCAGGCGTC TTTCCCATGA TGTCAGTGGG GCCCTGCTGT TGGCAAATGG GGAGAGCACT
    GGAAATTCTG GGGGCAGCAG TGGAAGCAGC CCGAGTGGGG GAGCCACCAG TGGGTCTTCC
    CAGACCAGTA TCTCAGGAGA TGTGGTGGAG GCATGCTGCA GCGTTCTGTC AATGGTCTGT
    GCTGACCCTG TCTACAAAGT GTACGTTGCT GCTTTAAAAA CATTGAGAGC CATGCTGGTA
    TATACTCCTT GCCACAGTTT AGCGGAAAGA ATCAAACTTC AGAGACTTCT CCAGCCAGTT
    GTAGACACCA TCCTAGTCAA ATGTGCAGAT GCCAATAGCC GCACAAGTCA GCTGTCCATA
    TCAACACTGT TGGAACTGTG CAAAGGCCAA GCAGGAGAGT TGGCAGTTGG CAGAGAAATA
    CTAAAAGCTG GATCCATTGG TATTGGTGGT GTTGATTATG TCTTAAATTG TATTCTTGGA
    AACCAAACTG AATCAAACAA TTGGCAAGAA CTTCTTGGCC GCCTTTGTCT TATAGATAGA
    CTGTTGTTGG AATTTCCTGC TGAATTTTAT CCTCATATTG TCAGTACTGA TGTTTCACAA
    GCTGAGCCTG TTGAAATCAG GTATAAGAAG CTGCTGTCCC TCTTAACCTT TGCTTTGCAG
    TCCATTGATA ATTCCCACTC AATGGTTGGC AAACTTTCCA GAAGGATCTA CTTGAGTTCT
    GCAAGAATGG TTACTACAGT ACCCCATGTG TTTTCAAAAC TGTTAGAAAT GCTGAGTGTT
    TCCAGTTCCA CTCACTTCAC CAGGATGCGT CGCCGTTTGA TGGCTATTGC AGATGAGGTG
    GAAATTGCCG AAGCCATCCA GTTGGGCGTA GAAGACACTT TGGATGGTCA ACAGGACAGC
    TTCTTGCAGG CATCTGTTCC CAACAACTAT CTGGAAACCA CAGAGAACAG TTCCCCTGAG
    TGCACAGTCC ATTTAGAGAA AACTGGAAAA GGATTATGTG CTACAAAATT GAGTGCCAGT
    TCAGAGGACA TTTCTGAGAG ACTGGCCAGC ATTTCAGTAG GACCTTCTAG TTCAACAACA
    ACAACAACAA CAACAACAGA GCAACCAAAG CCAATGGTTC AAACAAAAGG CAGACCCCAC
    AGTCAGTGTT TGAACTCCTC TCCTTTATCT CATCATTCCC AATTAATGTT TCCAGCCTTG
    TCAACCCCTT CTTCTTCTAC CCCATCTGTA CCAGCTGGCA CTGCAACAGA TGTCTCTAAG
    CATAGACTTC AGGGATTCAT TCCCTGCAGA ATACCTTCTG CATCTCCTCA AACACAGCGC
    AAGTTTTCTC TACAATTCCA CAGAAACTGT CCTGAAAACA AAGACTCAGA TAAACTTTCC
    CCAGTCTTTA CTCAGTCAAG ACCCTTGCCC TCCAGTAACA TACACAGGCC AAAGCCATCT
    AGACCTACCC CAGGTAATAC AAGTAAACAG GGAGATCCCT CAAAAAATAG CATGACACTT
    GATCTGAACA GTAGTTCCAA ATGTGATGAC AGCTTTGGCT GTAGCAGCAA TAGTAGTAAT
    GCTGTTATAC CCAGTGACGA GACAGTGTTC ACCCCAGTAG AGGAGAAATG CAGATTAGAT
    GTCAATACAG AGCTCAACTC CAGTATTGAG GACCTTCTTG AAGCATCTAT GCCTTCAAGT
    GATACAACAG TAACTTTTAA GTCAGAAGTT GCTGTCCTGT CTCCTGAAAA GGCTGAAAAT
    GATGATACCT ACAAAGATGA TGTGAATCAT AATCAAAAGT GCAAAGAGAA GATGGAAGCT
    GAAGAAGAAG AAGCTTTAGC AATTGCCATG GCAATGTCAG CGTCTCAGGA TGCCCTCCCC
    ATAGTTCCTC AGCTGCAGGT TGAAAATGGA GAAGATATCA TCATTATTCA ACAGGATACA
    CCAGAGACTC TACCAGGACA TACCAAAGCA AAACAACCGT ATAGAGAAGA CACTGAATGG
    CTGAAAGGTC AACAGATAGG CCTTGGAGCA TTTTCTTCTT GTTATCAGGC TCAAGATGTG
    GGAACTGGAA CTTTAATGGC TGTTAAACAG GTGACTTATG TCAGAAACAC ATCTTCTGAG
    CAAGAAGAAG TAGTAGAAGC ACTAAGAGAA GAGATAAGAA TGATGAGCCA TCTGAATCAT
    CCAAACATCA TTAGGATGTT GGGAGCCACG TGTGAGAAGA GCAATTACAA TCTCTTCATT
    GAATGGATGG CAGGGGGATC GGTGGCTCAT TTGCTGAGTA AATATGGAGC CTTCAAAGAA
    TCAGTAGTTA TTAACTACAC TGAACAGTTA CTCCGTGGCC TTTCGTATCT CCATGAAAAC
    CAAATCATTC ACAGAGATGT CAAAGGTGCC AATTTGCTAA TTGACAGCAC TGGTCAGAGA
    CTAAGAATTG CAGATTTTGG AGCTGCAGCC AGGTTGGCAT CAAAAGGAAC TGGTGCAGGA
    GAGTTTCAGG GACAATTACT GGGGACAATT GCATTTATGG CACCTGAGGT ACTAAGAGGT
    CAACAGTATG GAAGGAGCTG TGATGTATGG AGTGTTGGCT GTGCTATTAT AGAAATGGCT
    TGTGCAAAAC CACCATGGAA TGCAGAAAAA CACTCCAATC ATCTTGCTTT GATATTTAAG
    ATTGCTAGTG CAACTACTGC TCCATCGATC CCTTCACATT TGTCTCCTGG TTTACGAGAT
    GTGGCTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCATCAAG AGAGCTACTG
    AAGCATCCAG TCTTTCGTAC TACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95889
    Clone ID Related Accession (Same CDS sequence) XM_017009484.1
    Accession Version XM_017009484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACTTCT GCAAGGTGGT GGTCTTGAGT GGTGGAGAAA AAGATCATGA GTGCACAAGC 
    TGTTTCTGTC GTCGTGAGAT GGAGAATAAA GAAACTCTCA AAGGGTTGCA CAAGATGGAT
    GATCGTCCAG AGGAACGAAT GATCAGGGAG AAACTGAAGG CAACCTGTAT GCCAGCCTGG
    AAGCACGAAT GGTTGGAAAG GAGAAATAGG CGAGGGCCTG TGGTGGTAAA ACCAATCCCA
    GTTAAAGGAG ATGGATCTGA AATGAATCAC TTAGCAGCTG AGTCTCCAGG AGAGGTCCAG
    GCAAGTGCGG CTTCACCAGC TTCCAAAGGC CGACGCAGTC CTTCTCCTGG CAACTCCCCA
    TCAGGTCGCA CAGTGAAATC AGAATCTCCA GGAGTAAGGA GAAAAAGAGT TTCCCCAGTG
    CCTTTTCAGA GTGGCAGAAT CACACCACCC CGAAGAGCCC CTTCACCAGA TGGCTTCTCA
    CCATATAGCC CTGAGGAAAC AAACCGCCGT GTTAACAAAG TGATGCGGGC CAGACTGTAC
    TTACTGCAGC AGATAGGGCC TAACTCTTTC CTGATTGGAG GAGACAGCCC AGACAATAAA
    TACCGGGTGT TTATTGGGCC TCAGAACTGC AGCTGTGCAC GTGGAACATT CTGTATTCAT
    CTGCTATTTG TGATGCTCCG GGTGTTTCAA CTAGAACCTT CAGACCCAAT GTTATGGAGA
    AAAACTTTAA AGAATTTTGA GGTTGAGAGT TTGTTCCAGA AATATCACAG TAGGCGTAGC
    TCAAGGATCA AAGCTCCATC TCGTAACACC ATCCAGAAGT TTGTTTCACG CATGTCAAAT
    TCTCATACAT TGTCATCATC TAGTACTTCT ACGTCTAGTT CAGAAAACAG CATAAAGGAT
    GAAGAGGAAC AGATGTGTCC TATTTGCTTG TTGGGCATGC TTGATGAAGA AAGTCTTACA
    GTGTGTGAAG ACGGCTGCAG GAACAAGCTG CACCACCACT GCATGTCAAT TTGGGCAGAA
    GAGTGTAGAA GAAATAGAGA ACCTTTAATA TGTCCCCTTT GTAGATCTAA GTGGAGATCT
    CATGATTTCT ACAGCCACGA GTTGTCAAGT CCTGTGGATT CCCCTTCTTC CCTCAGAGCT
    GCACAGCAGC AAACCGTACA GCAGCAGCCT TTGGCTGGAT CACGAAGGAA TCAAGAGAGC
    AATTTTAACC TTACTCATTA TGGAACTCAG CAAATCCCTC CTGCTTACAA AGATTTAGCT
    GAGCCATGGA TTCAGGTGTT TGGAATGGAA CTCGTTGGCT GCTTATTTTC TAGAAACTGG
    AATGTGAGAG AGATGGCCCT CAGGCGTCTT TCCCATGATG TCAGTGGGGC CCTGCTGTTG
    GCAAATGGGG AGAGCACTGG AAATTCTGGG GGCAGCAGTG GAAGCAGCCC GAGTGGGGGA
    GCCACCAGTG GGTCTTCCCA GACCAGTATC TCAGGAGATG TGGTGGAGGC ATGCTGCAGC
    GTTCTGTCAA TGGTCTGTGC TGACCCTGTC TACAAAGTGT ACGTTGCTGC TTTAAAAACA
    TTGAGAGCCA TGCTGGTATA TACTCCTTGC CACAGTTTAG CGGAAAGAAT CAAACTTCAG
    AGACTTCTCC AGCCAGTTGT AGACACCATC CTAGTCAAAT GTGCAGATGC CAATAGCCGC
    ACAAGTCAGC TGTCCATATC AACACTGTTG GAACTGTGCA AAGGCCAAGC AGGAGAGTTG
    GCAGTTGGCA GAGAAATACT AAAAGCTGGA TCCATTGGTA TTGGTGGTGT TGATTATGTC
    TTAAATTGTA TTCTTGGAAA CCAAACTGAA TCAAACAATT GGCAAGAACT TCTTGGCCGC
    CTTTGTCTTA TAGATAGACT GTTGTTGGAA TTTCCTGCTG AATTTTATCC TCATATTGTC
    AGTACTGATG TTTCACAAGC TGAGCCTGTT GAAATCAGGT ATAAGAAGCT GCTGTCCCTC
    TTAACCTTTG CTTTGCAGTC CATTGATAAT TCCCACTCAA TGGTTGGCAA ACTTTCCAGA
    AGGATCTACT TGAGTTCTGC AAGAATGGTT ACTACAGTAC CCCATGTGTT TTCAAAACTG
    TTAGAAATGC TGAGTGTTTC CAGTTCCACT CACTTCACCA GGATGCGTCG CCGTTTGATG
    GCTATTGCAG ATGAGGTGGA AATTGCCGAA GCCATCCAGT TGGGCGTAGA AGACACTTTG
    GATGGTCAAC AGGACAGCTT CTTGCAGGCA TCTGTTCCCA ACAACTATCT GGAAACCACA
    GAGAACAGTT CCCCTGAGTG CACAGTCCAT TTAGAGAAAA CTGGAAAAGG ATTATGTGCT
    ACAAAATTGA GTGCCAGTTC AGAGGACATT TCTGAGAGAC TGGCCAGCAT TTCAGTAGGA
    CCTTCTAGTT CAACAACAAC AACAACAACA ACAACAGAGC AACCAAAGCC AATGGTTCAA
    ACAAAAGGCA GACCCCACAG TCAGTGTTTG AACTCCTCTC CTTTATCTCA TCATTCCCAA
    TTAATGTTTC CAGCCTTGTC AACCCCTTCT TCTTCTACCC CATCTGTACC AGCTGGCACT
    GCAACAGATG TCTCTAAGCA TAGACTTCAG GGATTCATTC CCTGCAGAAT ACCTTCTGCA
    TCTCCTCAAA CACAGCGCAA GTTTTCTCTA CAATTCCACA GAAACTGTCC TGAAAACAAA
    GACTCAGATA AACTTTCCCC AGTCTTTACT CAGTCAAGAC CCTTGCCCTC CAGTAACATA
    CACAGGCCAA AGCCATCTAG ACCTACCCCA GGTAATACAA GTAAACAGGG AGATCCCTCA
    AAAAATAGCA TGACACTTGA TCTGAACAGT AGTTCCAAAT GTGATGACAG CTTTGGCTGT
    AGCAGCAATA GTAGTAATGC TGTTATACCC AGTGACGAGA CAGTGTTCAC CCCAGTAGAG
    GAGAAATGCA GATTAGATGT CAATACAGAG CTCAACTCCA GTATTGAGGA CCTTCTTGAA
    GCATCTATGC CTTCAAGTGA TACAACAGTA ACTTTTAAGT CAGAAGTTGC TGTCCTGTCT
    CCTGAAAAGG CTGAAAATGA TGATACCTAC AAAGATGATG TGAATCATAA TCAAAAGTGC
    AAAGAGAAGA TGGAAGCTGA AGAAGAAGAA GCTTTAGCAA TTGCCATGGC AATGTCAGCG
    TCTCAGGATG CCCTCCCCAT AGTTCCTCAG CTGCAGGTTG AAAATGGAGA AGATATCATC
    ATTATTCAAC AGGATACACC AGAGACTCTA CCAGGACATA CCAAAGCAAA ACAACCGTAT
    AGAGAAGACA CTGAATGGCT GAAAGGTCAA CAGATAGGCC TTGGAGCATT TTCTTCTTGT
    TATCAGGCTC AAGATGTGGG AACTGGAACT TTAATGGCTG TTAAACAGGT GACTTATGTC
    AGAAACACAT CTTCTGAGCA AGAAGAAGTA GTAGAAGCAC TAAGAGAAGA GATAAGAATG
    ATGAGCCATC TGAATCATCC AAACATCATT AGGATGTTGG GAGCCACGTG TGAGAAGAGC
    AATTACAATC TCTTCATTGA ATGGATGGCA GGGGGATCGG TGGCTCATTT GCTGAGTAAA
    TATGGAGCCT TCAAAGAATC AGTAGTTATT AACTACACTG AACAGTTACT CCGTGGCCTT
    TCGTATCTCC ATGAAAACCA AATCATTCAC AGAGATGTCA AAGGTGCCAA TTTGCTAATT
    GACAGCACTG GTCAGAGACT AAGAATTGCA GATTTTGGAG CTGCAGCCAG GTTGGCATCA
    AAAGGAACTG GTGCAGGAGA GTTTCAGGGA CAATTACTGG GGACAATTGC ATTTATGGCA
    CCTGAGGTAC TAAGAGGTCA ACAGTATGGA AGGAGCTGTG ATGTATGGAG TGTTGGCTGT
    GCTATTATAG AAATGGCTTG TGCAAAACCA CCATGGAATG CAGAAAAACA CTCCAATCAT
    CTTGCTTTGA TATTTAAGAT TGCTAGTGCA ACTACTGCTC CATCGATCCC TTCACATTTG
    TCTCCTGGTT TACGAGATGT GGCTCTTCGT TGTTTAGAAC TTCAACCTCA GGACAGACCT
    CCATCAAGAG AGCTACTGAA GCATCCAGTC TTTCGTACTA CATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864973.1
    CDS3624..7751
    Translation

    Target ORF information:

    RefSeq Version XM_017009484.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACTTCT GCAAGGTGGT GGTCTTGAGT GGTGGAGAAA AAGATCATGA GTGCACAAGC 
    TGTTTCTGTC GTCGTGAGAT GGAGAATAAA GAAACTCTCA AAGGGTTGCA CAAGATGGAT
    GATCGTCCAG AGGAACGAAT GATCAGGGAG AAACTGAAGG CAACCTGTAT GCCAGCCTGG
    AAGCACGAAT GGTTGGAAAG GAGAAATAGG CGAGGGCCTG TGGTGGTAAA ACCAATCCCA
    GTTAAAGGAG ATGGATCTGA AATGAATCAC TTAGCAGCTG AGTCTCCAGG AGAGGTCCAG
    GCAAGTGCGG CTTCACCAGC TTCCAAAGGC CGACGCAGTC CTTCTCCTGG CAACTCCCCA
    TCAGGTCGCA CAGTGAAATC AGAATCTCCA GGAGTAAGGA GAAAAAGAGT TTCCCCAGTG
    CCTTTTCAGA GTGGCAGAAT CACACCACCC CGAAGAGCCC CTTCACCAGA TGGCTTCTCA
    CCATATAGCC CTGAGGAAAC AAACCGCCGT GTTAACAAAG TGATGCGGGC CAGACTGTAC
    TTACTGCAGC AGATAGGGCC TAACTCTTTC CTGATTGGAG GAGACAGCCC AGACAATAAA
    TACCGGGTGT TTATTGGGCC TCAGAACTGC AGCTGTGCAC GTGGAACATT CTGTATTCAT
    CTGCTATTTG TGATGCTCCG GGTGTTTCAA CTAGAACCTT CAGACCCAAT GTTATGGAGA
    AAAACTTTAA AGAATTTTGA GGTTGAGAGT TTGTTCCAGA AATATCACAG TAGGCGTAGC
    TCAAGGATCA AAGCTCCATC TCGTAACACC ATCCAGAAGT TTGTTTCACG CATGTCAAAT
    TCTCATACAT TGTCATCATC TAGTACTTCT ACGTCTAGTT CAGAAAACAG CATAAAGGAT
    GAAGAGGAAC AGATGTGTCC TATTTGCTTG TTGGGCATGC TTGATGAAGA AAGTCTTACA
    GTGTGTGAAG ACGGCTGCAG GAACAAGCTG CACCACCACT GCATGTCAAT TTGGGCAGAA
    GAGTGTAGAA GAAATAGAGA ACCTTTAATA TGTCCCCTTT GTAGATCTAA GTGGAGATCT
    CATGATTTCT ACAGCCACGA GTTGTCAAGT CCTGTGGATT CCCCTTCTTC CCTCAGAGCT
    GCACAGCAGC AAACCGTACA GCAGCAGCCT TTGGCTGGAT CACGAAGGAA TCAAGAGAGC
    AATTTTAACC TTACTCATTA TGGAACTCAG CAAATCCCTC CTGCTTACAA AGATTTAGCT
    GAGCCATGGA TTCAGGTGTT TGGAATGGAA CTCGTTGGCT GCTTATTTTC TAGAAACTGG
    AATGTGAGAG AGATGGCCCT CAGGCGTCTT TCCCATGATG TCAGTGGGGC CCTGCTGTTG
    GCAAATGGGG AGAGCACTGG AAATTCTGGG GGCAGCAGTG GAAGCAGCCC GAGTGGGGGA
    GCCACCAGTG GGTCTTCCCA GACCAGTATC TCAGGAGATG TGGTGGAGGC ATGCTGCAGC
    GTTCTGTCAA TGGTCTGTGC TGACCCTGTC TACAAAGTGT ACGTTGCTGC TTTAAAAACA
    TTGAGAGCCA TGCTGGTATA TACTCCTTGC CACAGTTTAG CGGAAAGAAT CAAACTTCAG
    AGACTTCTCC AGCCAGTTGT AGACACCATC CTAGTCAAAT GTGCAGATGC CAATAGCCGC
    ACAAGTCAGC TGTCCATATC AACACTGTTG GAACTGTGCA AAGGCCAAGC AGGAGAGTTG
    GCAGTTGGCA GAGAAATACT AAAAGCTGGA TCCATTGGTA TTGGTGGTGT TGATTATGTC
    TTAAATTGTA TTCTTGGAAA CCAAACTGAA TCAAACAATT GGCAAGAACT TCTTGGCCGC
    CTTTGTCTTA TAGATAGACT GTTGTTGGAA TTTCCTGCTG AATTTTATCC TCATATTGTC
    AGTACTGATG TTTCACAAGC TGAGCCTGTT GAAATCAGGT ATAAGAAGCT GCTGTCCCTC
    TTAACCTTTG CTTTGCAGTC CATTGATAAT TCCCACTCAA TGGTTGGCAA ACTTTCCAGA
    AGGATCTACT TGAGTTCTGC AAGAATGGTT ACTACAGTAC CCCATGTGTT TTCAAAACTG
    TTAGAAATGC TGAGTGTTTC CAGTTCCACT CACTTCACCA GGATGCGTCG CCGTTTGATG
    GCTATTGCAG ATGAGGTGGA AATTGCCGAA GCCATCCAGT TGGGCGTAGA AGACACTTTG
    GATGGTCAAC AGGACAGCTT CTTGCAGGCA TCTGTTCCCA ACAACTATCT GGAAACCACA
    GAGAACAGTT CCCCTGAGTG CACAGTCCAT TTAGAGAAAA CTGGAAAAGG ATTATGTGCT
    ACAAAATTGA GTGCCAGTTC AGAGGACATT TCTGAGAGAC TGGCCAGCAT TTCAGTAGGA
    CCTTCTAGTT CAACAACAAC AACAACAACA ACAACAGAGC AACCAAAGCC AATGGTTCAA
    ACAAAAGGCA GACCCCACAG TCAGTGTTTG AACTCCTCTC CTTTATCTCA TCATTCCCAA
    TTAATGTTTC CAGCCTTGTC AACCCCTTCT TCTTCTACCC CATCTGTACC AGCTGGCACT
    GCAACAGATG TCTCTAAGCA TAGACTTCAG GGATTCATTC CCTGCAGAAT ACCTTCTGCA
    TCTCCTCAAA CACAGCGCAA GTTTTCTCTA CAATTCCACA GAAACTGTCC TGAAAACAAA
    GACTCAGATA AACTTTCCCC AGTCTTTACT CAGTCAAGAC CCTTGCCCTC CAGTAACATA
    CACAGGCCAA AGCCATCTAG ACCTACCCCA GGTAATACAA GTAAACAGGG AGATCCCTCA
    AAAAATAGCA TGACACTTGA TCTGAACAGT AGTTCCAAAT GTGATGACAG CTTTGGCTGT
    AGCAGCAATA GTAGTAATGC TGTTATACCC AGTGACGAGA CAGTGTTCAC CCCAGTAGAG
    GAGAAATGCA GATTAGATGT CAATACAGAG CTCAACTCCA GTATTGAGGA CCTTCTTGAA
    GCATCTATGC CTTCAAGTGA TACAACAGTA ACTTTTAAGT CAGAAGTTGC TGTCCTGTCT
    CCTGAAAAGG CTGAAAATGA TGATACCTAC AAAGATGATG TGAATCATAA TCAAAAGTGC
    AAAGAGAAGA TGGAAGCTGA AGAAGAAGAA GCTTTAGCAA TTGCCATGGC AATGTCAGCG
    TCTCAGGATG CCCTCCCCAT AGTTCCTCAG CTGCAGGTTG AAAATGGAGA AGATATCATC
    ATTATTCAAC AGGATACACC AGAGACTCTA CCAGGACATA CCAAAGCAAA ACAACCGTAT
    AGAGAAGACA CTGAATGGCT GAAAGGTCAA CAGATAGGCC TTGGAGCATT TTCTTCTTGT
    TATCAGGCTC AAGATGTGGG AACTGGAACT TTAATGGCTG TTAAACAGGT GACTTATGTC
    AGAAACACAT CTTCTGAGCA AGAAGAAGTA GTAGAAGCAC TAAGAGAAGA GATAAGAATG
    ATGAGCCATC TGAATCATCC AAACATCATT AGGATGTTGG GAGCCACGTG TGAGAAGAGC
    AATTACAATC TCTTCATTGA ATGGATGGCA GGGGGATCGG TGGCTCATTT GCTGAGTAAA
    TATGGAGCCT TCAAAGAATC AGTAGTTATT AACTACACTG AACAGTTACT CCGTGGCCTT
    TCGTATCTCC ATGAAAACCA AATCATTCAC AGAGATGTCA AAGGTGCCAA TTTGCTAATT
    GACAGCACTG GTCAGAGACT AAGAATTGCA GATTTTGGAG CTGCAGCCAG GTTGGCATCA
    AAAGGAACTG GTGCAGGAGA GTTTCAGGGA CAATTACTGG GGACAATTGC ATTTATGGCA
    CCTGAGGTAC TAAGAGGTCA ACAGTATGGA AGGAGCTGTG ATGTATGGAG TGTTGGCTGT
    GCTATTATAG AAATGGCTTG TGCAAAACCA CCATGGAATG CAGAAAAACA CTCCAATCAT
    CTTGCTTTGA TATTTAAGAT TGCTAGTGCA ACTACTGCTC CATCGATCCC TTCACATTTG
    TCTCCTGGTT TACGAGATGT GGCTCTTCGT TGTTTAGAAC TTCAACCTCA GGACAGACCT
    CCATCAAGAG AGCTACTGAA GCATCCAGTC TTTCGTACTA CATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24943
    Clone ID Related Accession (Same CDS sequence) NM_005921.1 , NM_005921.2
    Accession Version NM_005921.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008937.7, AF042838.1, BU194120.1, DA889202.1 and AA602425.1. This sequence is a reference standard in the RefSeqGene project. On or before Jul 27, 2007 this sequence version replaced XM_042066.11, XM_001128827.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF042838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
    AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC
    GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT
    GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG
    AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC
    GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG
    GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA
    GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA
    TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA
    GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG
    GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC
    ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG
    AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG
    CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT
    GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA
    GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA
    AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC
    TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG
    CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC
    CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG
    ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA
    GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG
    GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT
    GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA
    ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC
    ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC
    CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG
    CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC
    AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT
    ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT
    ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT
    GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT
    GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC
    TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG
    CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA
    GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA
    CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT
    TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG
    AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT
    TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC
    AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT
    CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT
    GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA
    ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT
    AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA
    AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC
    ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT
    AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC
    AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG
    CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG
    GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG
    ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT
    GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA
    CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC
    ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT
    CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA
    TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT
    CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT
    CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC
    TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC
    CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT
    GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT
    GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA
    CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA
    GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG
    ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT
    TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA
    GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005912.1
    CDS502..5040
    Translation

    Target ORF information:

    RefSeq Version NM_005921.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
    AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC
    GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT
    GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG
    AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC
    GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG
    GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA
    GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA
    TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA
    GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG
    GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC
    ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG
    AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG
    CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT
    GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA
    GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA
    AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC
    TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG
    CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC
    CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG
    ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA
    GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG
    GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT
    GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA
    ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC
    ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC
    CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG
    CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC
    AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT
    ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT
    ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT
    GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT
    GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC
    TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG
    CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA
    GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA
    CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT
    TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG
    AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT
    TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC
    AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT
    CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT
    GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA
    ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT
    AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA
    AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC
    ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT
    AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC
    AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG
    CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG
    GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG
    ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT
    GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA
    CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC
    ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT
    CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA
    TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT
    CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT
    CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC
    TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC
    CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT
    GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT
    GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA
    CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA
    GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG
    ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT
    TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA
    GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24943
    Clone ID Related Accession (Same CDS sequence) NM_005921.1 , NM_005921.2
    Accession Version NM_005921.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008937.7, AF042838.1, BU194120.1, DA889202.1 and AA602425.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005921.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF042838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000399503.4/ ENSP00000382423.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
    AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC
    GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT
    GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG
    AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC
    GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG
    GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA
    GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA
    TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA
    GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG
    GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC
    ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG
    AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG
    CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT
    GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA
    GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA
    AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC
    TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG
    CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC
    CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG
    ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA
    GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG
    GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT
    GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA
    ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC
    ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC
    CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG
    CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC
    AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT
    ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT
    ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT
    GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT
    GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC
    TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG
    CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA
    GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA
    CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT
    TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG
    AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT
    TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC
    AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT
    CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT
    GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA
    ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT
    AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA
    AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC
    ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT
    AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC
    AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG
    CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG
    GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG
    ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT
    GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA
    CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC
    ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT
    CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA
    TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT
    CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT
    CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC
    TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC
    CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT
    GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT
    GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA
    CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA
    GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG
    ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT
    TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA
    GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005912.1
    CDS26..4564
    Translation

    Target ORF information:

    RefSeq Version NM_005921.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005921.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 
    AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC
    GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT
    GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG
    AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC
    GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG
    GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA
    GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA
    TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA
    GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG
    GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC
    ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG
    AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG
    CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT
    GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA
    GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA
    AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC
    TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG
    CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC
    CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG
    ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA
    GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG
    GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT
    GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA
    ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC
    ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC
    CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG
    CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC
    AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT
    ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT
    ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT
    GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT
    GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC
    TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG
    CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA
    GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA
    CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT
    TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG
    AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT
    TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC
    AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT
    CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT
    GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA
    ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT
    AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA
    AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC
    ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT
    AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC
    AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG
    CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG
    GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG
    ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT
    GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA
    CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC
    ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT
    CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA
    TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT
    CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT
    CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC
    TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC
    CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT
    GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT
    GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA
    CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA
    GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG
    ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT
    TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA
    GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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