ASAH1 cDNA ORF clone, Homo sapiens(Human)

  • Gene

  • Clones

  • gRNAs

The following ASAH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASAH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20258 NM_004315.6
Latest version!
Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu20264 NM_177924.5
Latest version!
Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu20041 NM_001127505.3
Latest version!
Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $230.30
$329.00
OHu42065 XM_005273504.3
Latest version!
Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu107073 NM_001363743.2
Latest version!
Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu20041 NM_001127505.2 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $230.30
$329.00
OHu20258 NM_004315.5 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu20264 NM_177924.4 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu107073 NM_001363743.1 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu20264 NM_177924.3 Homo sapiens N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20258
    Clone ID Related Accession (Same CDS sequence) NM_004315.5 , NM_004315.6
    Accession Version NM_004315.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product acid ceramidase isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1, AI241768.1 and AC124242.6. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_004315.5. Transcript Variant: This variant (2) encodes the longest isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.110388.1, SRR1660803.78057.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA
    GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA
    TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA
    AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA
    CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT
    ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT
    TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA
    GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT
    TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT
    GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT
    GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA
    GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA
    GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA
    GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA
    TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG
    AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC
    AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC
    AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC
    CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004306.3
    CDS161..1396
    Translation

    Target ORF information:

    RefSeq Version NM_004315.6
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004315.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA
    GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA
    TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA
    AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA
    CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT
    ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT
    TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA
    GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT
    TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT
    GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT
    GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA
    GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA
    GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA
    GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA
    TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG
    AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC
    AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC
    AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC
    CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20264
    Clone ID Related Accession (Same CDS sequence) NM_177924.5 , NM_177924.4 , NM_177924.3
    Accession Version NM_177924.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-12
    Organism Homo sapiens(human)
    Product acid ceramidase isoform a preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA306573.1, DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1, AI241768.1 and AC124242.6. On Nov 22, 2018 this sequence version replaced NM_177924.4. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 2. The resulting isoform (a) includes a predicted signal peptide, is shorter and has a distinct N-terminus compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.46311.1, SRR5189664.67191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000637790.2/ ENSP00000490272.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
    GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA
    CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA
    AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG
    AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA
    TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC
    GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT
    ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC
    ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG
    CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG
    GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT
    CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT
    CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC
    ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC
    TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA
    TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT
    TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT
    CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA
    AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA
    TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808592.2
    CDS50..1237
    Translation

    Target ORF information:

    RefSeq Version NM_177924.5
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177924.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
    GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA
    CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA
    AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG
    AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA
    TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC
    GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT
    ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC
    ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG
    CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG
    GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT
    CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT
    CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC
    ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC
    TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA
    TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT
    TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT
    CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA
    AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA
    TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20264
    Clone ID Related Accession (Same CDS sequence) NM_177924.5 , NM_177924.4 , NM_177924.3
    Accession Version NM_177924.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product acid ceramidase isoform a preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA306573.1, DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1 and AI241768.1. On Nov 5, 2015 this sequence version replaced NM_177924.3. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 2. The resulting isoform (a) includes a predicted signal peptide, is shorter and has a distinct N-terminus compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.60368.1, SRR1660803.13604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
    GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA
    CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA
    AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG
    AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA
    TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC
    GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT
    ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC
    ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG
    CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG
    GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT
    CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT
    CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC
    ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC
    TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA
    TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT
    TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT
    CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA
    AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA
    TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808592.2
    CDS313..1500
    Translation

    Target ORF information:

    RefSeq Version NM_177924.4
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177924.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
    GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA
    CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA
    AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG
    AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA
    TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC
    GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT
    ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC
    ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG
    CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG
    GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT
    CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT
    CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC
    ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC
    TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA
    TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT
    TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT
    CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA
    AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA
    TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20264
    Clone ID Related Accession (Same CDS sequence) NM_177924.5 , NM_177924.4 , NM_177924.3
    Accession Version NM_177924.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product acid ceramidase isoform a preproprotein
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
    GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA
    CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA
    AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG
    AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA
    TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC
    GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT
    ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC
    ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG
    CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG
    GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT
    CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT
    CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC
    ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC
    TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA
    TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT
    TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT
    CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA
    AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA
    TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808592.2
    Translation

    Target ORF information:

    RefSeq Version NM_177924.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
    GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA
    CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA
    AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG
    AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA
    TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC
    GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT
    ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC
    ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG
    CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG
    GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT
    CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT
    CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC
    ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC
    TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA
    TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT
    TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT
    CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA
    AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA
    TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20041
    Clone ID Related Accession (Same CDS sequence) NM_001127505.2 , NM_001127505.3
    Accession Version NM_001127505.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product acid ceramidase isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, BC016828.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1 and AI241768.1. On Nov 5, 2015 this sequence version replaced NM_001127505.1. Transcript Variant: This variant (3) contains an alternate in-frame exon and lacks another alternate in-frame exon compared to variant 2. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC
    CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA
    CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC
    AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT
    TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA
    GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT
    CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA
    GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC
    TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT
    TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC
    ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC
    AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC
    CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA
    CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT
    CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA
    GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG
    CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG
    GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120977.1
    CDS198..1367
    Translation

    Target ORF information:

    RefSeq Version NM_001127505.2
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC
    CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA
    CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC
    AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT
    TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA
    GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT
    CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA
    GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC
    TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT
    TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC
    ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC
    AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC
    CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA
    CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT
    CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA
    GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG
    CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG
    GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20041
    Clone ID Related Accession (Same CDS sequence) NM_001127505.2 , NM_001127505.3
    Accession Version NM_001127505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-12
    Organism Homo sapiens(human)
    Product acid ceramidase isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, BC016828.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1, AI241768.1 and AC124242.6. On May 31, 2019 this sequence version replaced NM_001127505.2. Transcript Variant: This variant (3) contains an alternate in-frame exon and lacks another alternate in-frame exon compared to variant 2. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC
    CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA
    CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC
    AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT
    TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA
    GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT
    CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA
    GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC
    TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT
    TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC
    ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC
    AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC
    CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA
    CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT
    CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA
    GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG
    CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG
    GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120977.1
    CDS161..1330
    Translation

    Target ORF information:

    RefSeq Version NM_001127505.3
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC
    CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA
    CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC
    AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT
    TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA
    GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT
    CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA
    GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC
    TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT
    TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC
    ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC
    AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC
    CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA
    CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT
    CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA
    GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG
    CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG
    GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20258
    Clone ID Related Accession (Same CDS sequence) NM_004315.5 , NM_004315.6
    Accession Version NM_004315.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product acid ceramidase isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1 and AI241768.1. This sequence is a reference standard in the RefSeqGene project. On Nov 5, 2015 this sequence version replaced NM_004315.4. Transcript Variant: This variant (2) encodes the longest isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.110417.1, SRR1660809.122258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA
    GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA
    TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA
    AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA
    CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT
    ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT
    TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA
    GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT
    TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT
    GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT
    GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA
    GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA
    GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA
    GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA
    TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG
    AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC
    AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC
    AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC
    CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004306.3
    CDS198..1433
    Translation

    Target ORF information:

    RefSeq Version NM_004315.5
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004315.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
    AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA
    GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA
    GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA
    TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA
    AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA
    CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT
    ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT
    TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA
    GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT
    TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT
    GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT
    GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA
    GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA
    GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA
    GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA
    TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG
    AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC
    AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC
    AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC
    CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42065
    Clone ID Related Accession (Same CDS sequence) XM_005273504.3
    Accession Version XM_005273504.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product acid ceramidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAGGCGC AGTGGACAGA GGACTGCAGA AAATCAACCT ATCCTCCTTC AGGACCAACG 
    TACAGAGGTG CAGTTCCATG GTACACCATA AATCTTGACT TACCACCCTA CAAAAGATGG
    CATGAATTGA TGCTTGACAA GGCACCAGTG CTAAAGGTTA TAGTGAATTC TCTGAAGAAT
    ATGATAAATA CATTCGTGCC AAGTGGAAAA ATTATGCAGG TGGTGGATGA AAAATTGCCT
    GGCCTACTTG GCAACTTTCC TGGCCCTTTT GAAGAGGAAA TGAAGGGTAT TGCCGCTGTT
    ACTGATATAC CTTTAGGAGA GATTATTTCA TTCAATATTT TTTATGAATT ATTTACCATT
    TGTACTTCAA TAGTAGCAGA AGACAAAAAA GGTCATCTAA TACATGGGAG AAACATGGAT
    TTTGGAGTAT TTCTTGGGTG GAACATAAAT AATGATACCT GGGTCATAAC TGAGCAACTA
    AAACCTTTAA CAGTGAATTT GGATTTCCAA AGAAACAACA AAACTGTCTT CAAGGCTTCA
    AGCTTTGCTG GCTATGTGGG CATGTTAACA GGATTCAAAC CAGGACTGTT CAGTCTTACA
    CTGAATGAAC GTTTCAGTAT AAATGGTGGT TATCTGGGTA TTCTAGAATG GATTCTGGGA
    AAGAAAGATG TCATGTGGAT AGGGTTCCTC ACTAGAACAG TTCTGGAAAA TAGCACAAGT
    TATGAAGAAG CCAAGAATTT ATTGACCAAG ACCAAGATAT TGGCCCCAGC CTACTTTATC
    CTGGGAGGCA ACCAGTCTGG GGAAGGTTGT GTGATTACAC GAGACAGAAA GGAATCATTG
    GATGTATATG AACTCGATGC TAAGCAGGGT AGATGGTATG TGGTACAAAC AAATTATGAC
    CGTTGGAAAC ATCCCTTCTT CCTTGATGAT CGCAGAACGC CTGCAAAGAT GTGTCTGAAC
    CGCACCAGCC AAGAGAATAT CTCATTTGAA ACCATGTATG ATGTCCTGTC AACAAAACCT
    GTCCTCAACA AGCTGACCGT ATACACAACC TTGATAGATG TTACCAAAGG TCAATTCGAA
    ACTTACCTGC GGGACTGCCC TGACCCTTGT ATAGGTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005273561.1
    CDS140..1261
    Translation

    Target ORF information:

    RefSeq Version XM_005273504.3
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005273504.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAGGCGC AGTGGACAGA GGACTGCAGA AAATCAACCT ATCCTCCTTC AGGACCAACG 
    TACAGAGGTG CAGTTCCATG GTACACCATA AATCTTGACT TACCACCCTA CAAAAGATGG
    CATGAATTGA TGCTTGACAA GGCACCAGTG CTAAAGGTTA TAGTGAATTC TCTGAAGAAT
    ATGATAAATA CATTCGTGCC AAGTGGAAAA ATTATGCAGG TGGTGGATGA AAAATTGCCT
    GGCCTACTTG GCAACTTTCC TGGCCCTTTT GAAGAGGAAA TGAAGGGTAT TGCCGCTGTT
    ACTGATATAC CTTTAGGAGA GATTATTTCA TTCAATATTT TTTATGAATT ATTTACCATT
    TGTACTTCAA TAGTAGCAGA AGACAAAAAA GGTCATCTAA TACATGGGAG AAACATGGAT
    TTTGGAGTAT TTCTTGGGTG GAACATAAAT AATGATACCT GGGTCATAAC TGAGCAACTA
    AAACCTTTAA CAGTGAATTT GGATTTCCAA AGAAACAACA AAACTGTCTT CAAGGCTTCA
    AGCTTTGCTG GCTATGTGGG CATGTTAACA GGATTCAAAC CAGGACTGTT CAGTCTTACA
    CTGAATGAAC GTTTCAGTAT AAATGGTGGT TATCTGGGTA TTCTAGAATG GATTCTGGGA
    AAGAAAGATG TCATGTGGAT AGGGTTCCTC ACTAGAACAG TTCTGGAAAA TAGCACAAGT
    TATGAAGAAG CCAAGAATTT ATTGACCAAG ACCAAGATAT TGGCCCCAGC CTACTTTATC
    CTGGGAGGCA ACCAGTCTGG GGAAGGTTGT GTGATTACAC GAGACAGAAA GGAATCATTG
    GATGTATATG AACTCGATGC TAAGCAGGGT AGATGGTATG TGGTACAAAC AAATTATGAC
    CGTTGGAAAC ATCCCTTCTT CCTTGATGAT CGCAGAACGC CTGCAAAGAT GTGTCTGAAC
    CGCACCAGCC AAGAGAATAT CTCATTTGAA ACCATGTATG ATGTCCTGTC AACAAAACCT
    GTCCTCAACA AGCTGACCGT ATACACAACC TTGATAGATG TTACCAAAGG TCAATTCGAA
    ACTTACCTGC GGGACTGCCC TGACCCTTGT ATAGGTTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107073
    Clone ID Related Accession (Same CDS sequence) NM_001363743.1 , NM_001363743.2
    Accession Version NM_001363743.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product acid ceramidase isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124242.6. On May 29, 2018 this sequence version replaced XM_024447153.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.124746.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267760, SAMN03267764 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
    ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT
    GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA
    CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA
    ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA
    TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA
    ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT
    GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA
    CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT
    GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA
    GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC
    AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT
    GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA
    CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC
    CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC
    AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG
    CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350672.1
    CDS193..1185
    Translation

    Target ORF information:

    RefSeq Version NM_001363743.1
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
    ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT
    GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA
    CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA
    ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA
    TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA
    ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT
    GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA
    CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT
    GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA
    GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC
    AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT
    GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA
    CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC
    CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC
    AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG
    CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107073
    Clone ID Related Accession (Same CDS sequence) NM_001363743.1 , NM_001363743.2
    Accession Version NM_001363743.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-12
    Organism Homo sapiens(human)
    Product acid ceramidase isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124242.6. On May 31, 2019 this sequence version replaced NM_001363743.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.124746.1, SRR1803616.178239.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267760, SAMN03267764 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
    ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT
    GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA
    CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA
    ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA
    TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA
    ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT
    GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA
    CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT
    GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA
    GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC
    AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT
    GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA
    CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC
    CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC
    AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG
    CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350672.1
    CDS193..1185
    Translation

    Target ORF information:

    RefSeq Version NM_001363743.2
    Organism Homo sapiens(human)
    Definition Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363743.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
    ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT
    GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA
    CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA
    ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA
    TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA
    ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT
    GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA
    CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT
    GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA
    GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC
    AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT
    GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA
    CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC
    CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC
    AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG
    CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.