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MYL1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MYL1
Entrez Gene ID 4632
Full Name myosin light chain 1
Synonyms MLC1F,MLC3F
General protein information
Preferred Names

myosin light chain 1

Names

myosin light chain 1/3, skeletal muscle isoform
A1 catalytic
A2 catalytic
MLC1/MLC3
MLC1F/MLC3F
myosin light chain A1/A2
myosin light chain alkali 1/2
myosin, light chain 1, alkali; skeletal, fast
myosin, light polypeptide 1, alkali; skeletal, fast

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2q34

Summary Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

160780


mRNA and Protein(s)

mRNA Protein Name
NM_079422.2 NP_524146.1 myosin light chain 1/3, skeletal muscle isoform isoform 3f
NM_079420.2 NP_524144.1 myosin light chain 1/3, skeletal muscle isoform isoform 1f



Rattus norvegicus (Norway rat) Myl1 NP_001071124.1
Pan troglodytes (chimpanzee) MYL1 XP_516064.2
Canis lupus familiaris (dog) MYL1 XP_005640670.1
Mus musculus (house mouse) Myl1 NP_067260.1
Homo sapiens (human) MYL1 NP_524144.1
Bos taurus (cattle) MYL1 NP_001073046.1
Danio rerio (zebrafish) myl1 NP_956294.1
Kluyveromyces lactis KLLA0F10439g XP_455555.1
Schizosaccharomyces pombe (fission yeast) cdc4 NP_594947.1
Neurospora crassa NCU06617 XP_962359.2
Danio rerio (zebrafish) mylz3 NP_571694.1
Saccharomyces cerevisiae (baker's yeast) MLC1 NP_011409.1
Magnaporthe oryzae (rice blast fungus) MGG_09470 XP_003712281.1
Gallus gallus (chicken) MYL1 NP_001038097.1
Eremothecium gossypii AGOS_AFL030C NP_985518.1
Xenopus tropicalis (tropical clawed frog) myl1 NP_988954.1


Related articles in PubMed

Intron polymorphism in MYL1 gene is associated with individual cardiac trainability to endurance training in human myocardium.
Lin X, Wang D, Wen L, Zhou S, Hu Y, Zhang Y
The Journal of sports medicine and physical fitness57(1-2)144-153(2017 Jan-Feb)

Src Family Kinases (SFK) Mediate Angiotensin II-Induced Myosin Light Chain Phosphorylation and Hypertension.
Qin B, Zhou J
PloS one10(5)e0127891(2015)

The MLK-related kinase (MRK) is a novel RhoC effector that mediates lysophosphatidic acid (LPA)-stimulated tumor cell invasion.
Korkina O, Dong Z, Marullo A, Warshaw G, Symons M, Ruggieri R
The Journal of biological chemistry288(8)5364-73(2013 Feb)

Mutations of ventricular essential myosin light chain disturb myosin binding and sarcomeric sorting.
Lossie J, Ushakov DS, Ferenczi MA, Werner S, Keller S, Haase H, Morano I
Cardiovascular research93(3)390-6(2012 Mar)

Inhibition of MLC phosphorylation restricts replication of influenza virus--a mechanism of action for anti-influenza agents.
Haidari M, Zhang W, Ganjehei L, Ali M, Chen Z
PloS one6(6)e21444(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

polymorphism of intron MYL1 was associated with endurance performance, specifically in endurance trainability, but not genetic predisposition, in human adults.
Title: Intron polymorphism in MYL1 gene is associated with individual cardiac trainability to endurance training in human myocardium.

This study provides an additional mechanistic link between Angiotensin II and vasoconstriction via SFK-enhanced MLC phosphorylation in smooth muscle cells.
Title: Src Family Kinases (SFK) Mediate Angiotensin II-Induced Myosin Light Chain Phosphorylation and Hypertension.

MRK is a novel RhoC effector that controls LPA-stimulated cell invasion at least in part by regulating myosin dynamics, ERK and p38
Title: The MLK-related kinase (MRK) is a novel RhoC effector that mediates lysophosphatidic acid (LPA)-stimulated tumor cell invasion.

Disturbed myosin binding of mutated hVLC-1 may provide a pathomechanism for the development of hypertrohic cardiomyopathy.
Title: Mutations of ventricular essential myosin light chain disturb myosin binding and sarcomeric sorting.

Influenza infection activates a series of signaling pathways that converge to induce myosin light chain (MLC) phosphorylation and remodeling of the actin cytoskeleton.
Title: Inhibition of MLC phosphorylation restricts replication of influenza virus--a mechanism of action for anti-influenza agents.

The following MYL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu13487 NM_079422.2
Latest version!
Homo sapiens myosin light chain 1 (MYL1), transcript variant 3f, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu13342 NM_079420.2
Latest version!
Homo sapiens myosin light chain 1 (MYL1), transcript variant 1f, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

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Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13487
Clone ID Related Accession (Same CDS sequence) NM_079422.2
Accession Version NM_079422.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-28
Organism Homo sapiens(Human)
Product myosin light chain 1/3, skeletal muscle isoform isoform 3f
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005318.1 and AA705167.1. On Oct 4, 2006 this sequence version replaced NM_079422.1. Transcript Variant: This variant (3f) encodes a protein that is 44 aa shorter than isoform 1f. Transcript variants 3f and 1f differ in their first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005318.1, BX436371.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
ATGTCCTTCA GTGCTGACCA GATTGCTGAA TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGATT CCAAGATCAC CTTAAGCCAG GTCGGTGATG TCCTTCGAGC TCTGGGCACA
AATCCCACCA ATGCAGAGGT CAGGAAAGTT CTGGGAAACC CCAGCAATGA AGAGCTGAAT
GCCAAGAAAA TTGAGTTTGA ACAATTTCTG CCTATGATGC AAGCCATTTC CAACAACAAG
GACCAGGCCA CCTATGAAGA CTTTGTTGAG GGTCTGCGTG TCTTTGACAA GGAAGGCAAT
GGCACAGTCA TGGGTGCTGA ACTCCGCCAT GTTCTAGCCA CCCTGGGTGA AAAGATGAAA
GAGGAAGAAG TGGAAGCCCT GATGGCAGGT CAAGAAGACT CCAATGGCTG CATCAACTAC
GAAGCTTTTG TCAAGCACAT CATGTCTATC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_524146.1
CDS72..524
Misc Feature(1)81..518
Misc Feature(2)99..293
Misc Feature(3)99..188
Misc Feature(4)330..515
Misc Feature(5)order(357..359,363..365,369..371,390..392,462..464,468..470,474..476,495..497)
Exon (1)1..74
Exon (2)75..99
Exon (3)100..243
Exon (4)244..417
Exon (5)418..495
Exon (6)496..538
Exon (7)539..862
Translation

Target ORF information:

RefSeq Version NM_079422.2
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 1 (MYL1), transcript variant 3f, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_079422.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGTCCTTCA GTGCTGACCA GATTGCTGAA TTCAAGGAGG CATTTCTCCT GTTTGACAGA 
ACAGGTGATT CCAAGATCAC CTTAAGCCAG GTCGGTGATG TCCTTCGAGC TCTGGGCACA
AATCCCACCA ATGCAGAGGT CAGGAAAGTT CTGGGAAACC CCAGCAATGA AGAGCTGAAT
GCCAAGAAAA TTGAGTTTGA ACAATTTCTG CCTATGATGC AAGCCATTTC CAACAACAAG
GACCAGGCCA CCTATGAAGA CTTTGTTGAG GGTCTGCGTG TCTTTGACAA GGAAGGCAAT
GGCACAGTCA TGGGTGCTGA ACTCCGCCAT GTTCTAGCCA CCCTGGGTGA AAAGATGAAA
GAGGAAGAAG TGGAAGCCCT GATGGCAGGT CAAGAAGACT CCAATGGCTG CATCAACTAC
GAAGCTTTTG TCAAGCACAT CATGTCTATC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13342
Clone ID Related Accession (Same CDS sequence) NM_079420.2
Accession Version NM_079420.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-28
Organism Homo sapiens(Human)
Product myosin light chain 1/3, skeletal muscle isoform isoform 1f
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA898482.1, CR456869.1 and AA705167.1. On Oct 4, 2006 this sequence version replaced NM_079420.1. Transcript Variant: This variant (1f) encodes a protein that is 44 aa longer than isoform 3f. Transcript variants 1f and 3f differ in their first two exons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279835.5629.1, ERR279856.3421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
ATGGCACCAA AGAAAGACGT GAAGAAACCT GTGGCTGCGG CTGCGGCTGC CCCAGCCCCG 
GCACCGGCAC CTGCACCTGC CCCTGCCCCA GCCAAACCCA AAGAAGAAAA AATTGACCTC
TCTGCCATTA AGATCGAGTT CTCTAAGGAA CAGCAGGATG AATTCAAGGA GGCATTTCTC
CTGTTTGACA GAACAGGTGA TTCCAAGATC ACCTTAAGCC AGGTCGGTGA TGTCCTTCGA
GCTCTGGGCA CAAATCCCAC CAATGCAGAG GTCAGGAAAG TTCTGGGAAA CCCCAGCAAT
GAAGAGCTGA ATGCCAAGAA AATTGAGTTT GAACAATTTC TGCCTATGAT GCAAGCCATT
TCCAACAACA AGGACCAGGC CACCTATGAA GACTTTGTTG AGGGTCTGCG TGTCTTTGAC
AAGGAAGGCA ATGGCACAGT CATGGGTGCT GAACTCCGCC ATGTTCTAGC CACCCTGGGT
GAAAAGATGA AAGAGGAAGA AGTGGAAGCC CTGATGGCAG GTCAAGAAGA CTCCAATGGC
TGCATCAACT ACGAAGCTTT TGTCAAGCAC ATCATGTCTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_524144.1
CDS130..714
Misc Feature(1)106..108
Misc Feature(2)133..135
Misc Feature(3)262..708
Misc Feature(4)289..483
Misc Feature(5)289..378
Misc Feature(6)340..342
Misc Feature(7)346..348
Misc Feature(8)388..390
Misc Feature(9)424..426
Misc Feature(10)520..705
Misc Feature(11)order(547..549,553..555,559..561,580..582,652..654,658..660,664..666,685..687)
Exon (1)1..261
Exon (2)262..289
Exon (3)290..433
Exon (4)434..607
Exon (5)608..685
Exon (6)686..728
Exon (7)729..1052
Translation

Target ORF information:

RefSeq Version NM_079420.2
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 1 (MYL1), transcript variant 1f, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_079420.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCACCAA AGAAAGACGT GAAGAAACCT GTGGCTGCGG CTGCGGCTGC CCCAGCCCCG 
GCACCGGCAC CTGCACCTGC CCCTGCCCCA GCCAAACCCA AAGAAGAAAA AATTGACCTC
TCTGCCATTA AGATCGAGTT CTCTAAGGAA CAGCAGGATG AATTCAAGGA GGCATTTCTC
CTGTTTGACA GAACAGGTGA TTCCAAGATC ACCTTAAGCC AGGTCGGTGA TGTCCTTCGA
GCTCTGGGCA CAAATCCCAC CAATGCAGAG GTCAGGAAAG TTCTGGGAAA CCCCAGCAAT
GAAGAGCTGA ATGCCAAGAA AATTGAGTTT GAACAATTTC TGCCTATGAT GCAAGCCATT
TCCAACAACA AGGACCAGGC CACCTATGAA GACTTTGTTG AGGGTCTGCG TGTCTTTGAC
AAGGAAGGCA ATGGCACAGT CATGGGTGCT GAACTCCGCC ATGTTCTAGC CACCCTGGGT
GAAAAGATGA AAGAGGAAGA AGTGGAAGCC CTGATGGCAG GTCAAGAAGA CTCCAATGGC
TGCATCAACT ACGAAGCTTT TGTCAAGCAC ATCATGTCTA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Intron polymorphism in MYL1 gene is associated with individual cardiac trainability to endurance training in human myocardium.
The Journal of sports medicine and physical fitness57(1-2)144-153(2017 Jan-Feb)
Lin X,Wang D,Wen L,Zhou S,Hu Y,Zhang Y


book

Src Family Kinases (SFK) Mediate Angiotensin II-Induced Myosin Light Chain Phosphorylation and Hypertension.
PloS one10(5)e0127891(2015)
Qin B,Zhou J


book

The MLK-related kinase (MRK) is a novel RhoC effector that mediates lysophosphatidic acid (LPA)-stimulated tumor cell invasion.
The Journal of biological chemistry288(8)5364-73(2013 Feb)
Korkina O,Dong Z,Marullo A,Warshaw G,Symons M,Ruggieri R


book

Mutations of ventricular essential myosin light chain disturb myosin binding and sarcomeric sorting.
Cardiovascular research93(3)390-6(2012 Mar)
Lossie J,Ushakov DS,Ferenczi MA,Werner S,Keller S,Haase H,Morano I


book

Inhibition of MLC phosphorylation restricts replication of influenza virus--a mechanism of action for anti-influenza agents.
PloS one6(6)e21444(2011)
Haidari M,Zhang W,Ganjehei L,Ali M,Chen Z


 
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