ATF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATF4
Entrez Gene ID	468
Full Name	activating transcription factor 4
Synonyms	CREB-2,CREB2,TAXREB67,TXREB
General protein information	Preferred Names activating transcription factor 4 Names cyclic AMP-dependent transcription factor ATF-4 DNA-binding protein TAXREB67 cAMP response element-binding protein 2 cAMP-dependent transcription factor ATF-4 cAMP-responsive element-binding protein 2 cyclic AMP-responsive element-binding protein 2 tax-responsive enhancer element B67 tax-responsive enhancer element-binding protein 67
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.1
Summary	This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011].
Disorder MIM:	604064

mRNA and Protein(s)

mRNA	Protein	Name
XM_017028807.1	XP_016884296.1	cyclic AMP-dependent transcription factor ATF-4 isoform X1
XM_017028807.2	XP_016884296.1	cyclic AMP-dependent transcription factor ATF-4 isoform X1
NM_182810.2	NP_877962.1	cyclic AMP-dependent transcription factor ATF-4
NM_001675.4	NP_001666.2	cyclic AMP-dependent transcription factor ATF-4

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATF4	NP_001666.2
Canis lupus familiaris (dog)	ATF4	XP_859677.1
Mus musculus (house mouse)	Atf4	NP_001274109.1
Danio rerio (zebrafish)	atf4b2	NP_001096662.1
Xenopus tropicalis (tropical clawed frog)	atf4	NP_001004785.1
Danio rerio (zebrafish)	atf4b1	NP_998398.1
Pan troglodytes (chimpanzee)	ATF4	NP_001239446.1
	ATF4	XP_002798402.1
Bos taurus (cattle)	ATF4	NP_001029514.1
Rattus norvegicus (Norway rat)	Atf4	NP_077379.1
Gallus gallus (chicken)	ATF4	NP_990211.1

Gene Ontology
Bibliography

Related articles in PubMed

Translation of 5' leaders is pervasive in genes resistant to eIF2 repression.
Andreev DE, O'Connor PB, Fahey C, Kenny EM, Terenin IM, Dmitriev SE, Cormican P, Morris DW, Shatsky IN, Baranov PV
eLife4e03971(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GRP78 inhibition enhances ATF4-induced cell death by the deubiquitination and stabilization of CHOP in human osteosarcoma cells.
Title: GRP78 inhibition enhances ATF4-induced cell death by the deubiquitination and stabilization of CHOP in human osteosarcoma.

These results suggest that p21 induction plays a vital role in the cellular response to ER stress and indicate that p21 is a prosurvival effector of ATF4.
Title: The CDK inhibitor p21 is a novel target gene of ATF4 and contributes to cell survival under ER stress.

ATF4 contributes to tumor growth of endometrial cancer (EC) by promoting CCL2 and subsequent recruitment of macrophage, and ATF4/CCL2 axis might be a potential therapeutic target for EC.
Title: ATF4 regulates CCL2 expression to promote endometrial cancer growth by controlling macrophage infiltration.

SLC30A10 has a protective role in 1-methyl-4-phenylpyridinium-induced toxicity via PERK-ATF4 pathway.
Title: Protective function of SLC30A10 induced via PERK-ATF4 pathway against 1-methyl-4-phenylpyridinium.

ATF4 pathway is activated in vivo upon mitochondrial stress.
Title: Multi-omics analysis identifies ATF4 as a key regulator of the mitochondrial stress response in mammals.

The following ATF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14189	NM_001675.4 Latest version!	Homo sapiens activating transcription factor 4 (ATF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14189	NM_182810.2 Latest version!	Homo sapiens activating transcription factor 4 (ATF4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14189	XM_017028807.2 Latest version!	Homo sapiens activating transcription factor 4 (ATF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu14189	XM_017028807.1	Homo sapiens activating transcription factor 4 (ATF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATF4 ( NM_182810.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_877962.1 Homo sapiens activating transcription factor 4 (ATF4), transcript variant 2, mRNA.

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CloneID	OHu14189
Clone ID Related Accession (Same CDS sequence)	XM_017028807.1 , XM_017028807.2 , NM_182810.2 , NM_001675.4
Accession Version	NM_182810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cyclic AMP-dependent transcription factor ATF-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057751.1 and BC024775.1. On Dec 4, 2013 this sequence version replaced NM_182810.1. Transcript Variant: This variant (2) lacks an internal segment in the 5' UTR, compared to variant 1. The protein translation of this variant is regulated by two upstream open reading frames (PMID: 15277680). Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.254576.1, SRR1163655.513096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 15277680 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_877962.1
CDS	281..1336
Translation	MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

Target ORF information:

RefSeq Version	NM_182810.2
Organism	Homo sapiens(human)
Definition	Homo sapiens activating transcription factor 4 (ATF4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182810.2

ORF Insert Sequence:

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATF4 ( NM_001675.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001666.2 Homo sapiens activating transcription factor 4 (ATF4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14189
Clone ID Related Accession (Same CDS sequence)	XM_017028807.1 , XM_017028807.2 , NM_182810.2 , NM_001675.4
Accession Version	NM_001675.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cyclic AMP-dependent transcription factor ATF-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022312.7, D90209.1 and BC011994.1. On Feb 11, 2014 this sequence version replaced NM_001675.3. Transcript Variant: This variant (1) represents the longer transcript. The protein translation of this variant is regulated by an internal ribosome entry site (PMID: 23665047). Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.547543.1, SRR1163655.386267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001666.2
CDS	888..1943
Translation	MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

Target ORF information:

RefSeq Version	NM_001675.4
Organism	Homo sapiens(human)
Definition	Homo sapiens activating transcription factor 4 (ATF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001675.4

ORF Insert Sequence:

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATF4 ( XM_017028807.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884296.1 Homo sapiens activating transcription factor 4 (ATF4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu14189
Clone ID Related Accession (Same CDS sequence)	XM_017028807.1 , XM_017028807.2 , NM_182810.2 , NM_001675.4
Accession Version	XM_017028807.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cyclic AMP-dependent transcription factor ATF-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884296.1
CDS	760..1815
Translation	MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

Target ORF information:

RefSeq Version	XM_017028807.1
Organism	Homo sapiens(human)
Definition	Homo sapiens activating transcription factor 4 (ATF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028807.1

ORF Insert Sequence:

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATF4 ( XM_017028807.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884296.1 Homo sapiens activating transcription factor 4 (ATF4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu14189
Clone ID Related Accession (Same CDS sequence)	XM_017028807.1 , XM_017028807.2 , NM_182810.2 , NM_001675.4
Accession Version	XM_017028807.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cyclic AMP-dependent transcription factor ATF-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884296.1
CDS	5975..7030
Translation	MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

Target ORF information:

RefSeq Version	XM_017028807.2
Organism	Homo sapiens(human)
Definition	Homo sapiens activating transcription factor 4 (ATF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028807.2

ORF Insert Sequence:

ATGACCGAAA TGAGCTTCCT GAGCAGCGAG GTGTTGGTGG GGGACTTGAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGATTA CCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CTAAGGCGGG CTCCTCCGAA 
TGGCTGGCTG TGGATGGGTT GGTCAGTCCC TCCAACAACA GCAAGGAGGA TGCCTTCTCC 
GGGACAGATT GGATGTTGGA GAAAATGGAT TTGAAGGAGT TCGACTTGGA TGCCCTGTTG 
GGTATAGATG ACCTGGAAAC CATGCCAGAT GACCTTCTGA CCACGTTGGA TGACACTTGT 
GATCTCTTTG CCCCCCTAGT CCAGGAGACT AATAAGCAGC CCCCCCAGAC GGTGAACCCA 
ATTGGCCATC TCCCAGAAAG TTTAACAAAA CCCGACCAGG TTGCCCCCTT CACCTTCTTA 
CAACCTCTTC CCCTTTCCCC AGGGGTCCTG TCCTCCACTC CAGATCATTC CTTTAGTTTA 
GAGCTGGGCA GTGAAGTGGA TATCACTGAA GGAGATAGGA AGCCAGACTA CACTGCTTAC 
GTTGCCATGA TCCCTCAGTG CATAAAGGAG GAAGACACCC CTTCAGATAA TGATAGTGGC 
ATCTGTATGA GCCCAGAGTC CTATCTGGGG TCTCCTCAGC ACAGCCCCTC TACCAGGGGC 
TCTCCAAATA GGAGCCTCCC ATCTCCAGGT GTTCTCTGTG GGTCTGCCCG TCCCAAACCT 
TACGATCCTC CTGGAGAGAA GATGGTAGCA GCAAAAGTAA AGGGTGAGAA ACTGGATAAG 
AAGCTGAAAA AAATGGAGCA AAACAAGACA GCAGCCACTA GGTACCGCCA GAAGAAGAGG 
GCGGAGCAGG AGGCTCTTAC TGGTGAGTGC AAAGAGCTGG AAAAGAAGAA CGAGGCTCTA 
AAAGAGAGGG CGGATTCCCT GGCCAAGGAG ATCCAGTACC TGAAAGATTT GATAGAAGAG 
GTCCGCAAGG CAAGGGGGAA GAAAAGGGTC CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Translation of 5' leaders is pervasive in genes resistant to eIF2 repression.
eLife4e03971(2015 Jan)
Andreev DE,O'Connor PB,Fahey C,Kenny EM,Terenin IM,Dmitriev SE,Cormican P,Morris DW,Shatsky IN,Baranov PV

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ATF4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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