NDP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDP
Entrez Gene ID	4693
Full Name	NDP, norrin cystine knot growth factor
Synonyms	EVR2,FEVR,ND
General protein information	Preferred Names NDP, norrin cystine knot growth factor Names norrin Norrie disease (pseudoglioma) X-linked exudative vitreoretinopathy 2 protein norrie disease protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.3
Summary	This gene encodes a secreted protein with a cystein-knot motif that activates the Wnt/beta-catenin pathway. The protein forms disulfide-linked oligomers in the extracellular matrix. Mutations in this gene result in Norrie disease and X-linked exudative vitreoretinopathy. [provided by RefSeq, Feb 2009].
Disorder MIM:	300658
Disorder Html:	Norrie disease, 310600 (3); Exudative vitreoretinopathy 2, X-linked, 305390 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000266.4	NP_000257.1	norrin precursor
NM_000266.3	NP_000257.1	norrin precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NDP	XP_001139783.1
Bos taurus (cattle)	NDP	NP_001039555.1
	NDP	XP_002806251.1
Canis lupus familiaris (dog)	NDP	XP_855261.1
Gallus gallus (chicken)	NDP	NP_001265015.1
Danio rerio (zebrafish)	LOC100003793	XP_001338820.2
Homo sapiens (human)	NDP	NP_000257.1
Mus musculus (house mouse)	Ndp	NP_035013.1
Xenopus tropicalis (tropical clawed frog)	ndp	XP_002932837.1

Gene Ontology
Bibliography

Related articles in PubMed

Next-generation sequencing reveals a novel NDP gene mutation in a Chinese family with Norrie disease.
Huang X, Tian M, Li J, Cui L, Li M, Zhang J
Indian journal of ophthalmology65(11)1161-1165(2017 Nov)

Mutation Spectrum of the LRP5, NDP, and TSPAN12 Genes in Chinese Patients With Familial Exudative Vitreoretinopathy.
Tang M, Sun L, Hu A, Yuan M, Yang Y, Peng X, Ding X
Investigative ophthalmology & visual science58(13)5949-5957(2017 Nov)

Mutations in LRP5,FZD4, TSPAN12, NDP, ZNF408, or KIF11 Genes Account for 38.7% of Chinese Patients With Familial Exudative Vitreoretinopathy.
Rao FQ, Cai XB, Cheng FF, Cheng W, Fang XL, Li N, Huang XF, Li LH, Jin ZB
Investigative ophthalmology & visual science58(5)2623-2629(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

c.314C>A mutation of NDP gene is a novel mutation and broadens the genetic spectrum of Norrie disease.
Title: Next-generation sequencing reveals a novel NDP gene mutation in a Chinese family with Norrie disease.

Probands with LRP5 or NDP mutations were mainly categorized into group III and IV, TSPAN12 mutations were mainly observed in probands with group IV and V FEVR.
Title: Mutation Spectrum of the LRP5, NDP, and TSPAN12 Genes in Chinese Patients With Familial Exudative Vitreoretinopathy.

Among the detected mutations, LRP5 accounted for the largest proportion with a mean mutation rate of 16.1% (5/31, 16.1%), followed by NDP (3/31, 9.7%), FZD4 (2/31, 6.5%), TSPAN12 (1/31, 3.2%), and KIF11 (1/31, 3.2%). All the novel changes were predicted to be pathogenic by a series of bioinformatics analyses.
Title: Mutations in LRP5,FZD4, TSPAN12, NDP, ZNF408, or KIF11 Genes Account for 38.7% of Chinese Patients With Familial Exudative Vitreoretinopathy.

The mutation c.310A>C (p.Lys104Gln) in exon 3 of NDP is associated with familial exudative vitreoretinopathy in the studied family
Title: A novel missense mutation of NDP in a Chinese family with X-linked familial exudative vitreoretinopathy.

First study to demonstrate the involvement of NDP among patients with Indian familial exudative vitreoretinopathy (FEVR) that further expands its mutation spectrum.
Title: Mutation spectrum of the Norrie disease pseudoglioma (NDP) gene in Indian patients with FEVR.

The following NDP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26248	NM_000266.4 Latest version!	Homo sapiens norrin cystine knot growth factor NDP (NDP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu26248	NM_000266.3	Homo sapiens NDP, norrin cystine knot growth factor (NDP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDP ( NM_000266.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000257.1 Homo sapiens norrin cystine knot growth factor NDP (NDP), mRNA.

Price & Availability↑

CloneID	OHu26248
Clone ID Related Accession (Same CDS sequence)	NM_000266.4 , NM_000266.3
Accession Version	NM_000266.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	norrin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X65882.1 and BC029901.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000266.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1017256.1, SRR3476690.582694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000642620.1/ ENSP00000495972.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGAAAAC ATGTACTAGC TGCATCCTTT TCTATGCTCT CCCTGCTGGT GATAATGGGA 
GATACAGACA GTAAAACGGA CAGCTCATTC ATAATGGACT CGGACCCTCG ACGCTGCATG 
AGGCACCACT ATGTGGATTC TATCAGTCAC CCATTGTACA AGTGTAGCTC AAAGATGGTG 
CTCCTGGCCA GGTGCGAGGG GCACTGCAGC CAGGCGTCAC GCTCCGAGCC TTTGGTGTCG 
TTCAGCACTG TCCTCAAGCA ACCCTTCCGT TCCTCCTGTC ACTGCTGCCG GCCCCAGACT 
TCCAAGCTGA AGGCACTGCG GCTGCGATGC TCAGGGGGCA TGCGACTCAC TGCCACCTAC 
CGGTACATCC TCTCCTGTCA CTGCGAGGAA TGCAATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000257.1
CDS	295..696
Translation	MRKHVLAASFSMLSLLVIMGDTDSKTDSSFIMDSDPRRCMRHHYVDSISHPLYKCSSKMVLLARCEGHCSQASRSEPLVSFSTVLKQPFRSSCHCCRPQTSKLKALRLRCSGGMRLTATYRYILSCHCEECNS

Target ORF information:

RefSeq Version	NM_000266.4
Organism	Homo sapiens(human)
Definition	Homo sapiens norrin cystine knot growth factor NDP (NDP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000266.4

ORF Insert Sequence:

ATGAGAAAAC ATGTACTAGC TGCATCCTTT TCTATGCTCT CCCTGCTGGT GATAATGGGA 
GATACAGACA GTAAAACGGA CAGCTCATTC ATAATGGACT CGGACCCTCG ACGCTGCATG 
AGGCACCACT ATGTGGATTC TATCAGTCAC CCATTGTACA AGTGTAGCTC AAAGATGGTG 
CTCCTGGCCA GGTGCGAGGG GCACTGCAGC CAGGCGTCAC GCTCCGAGCC TTTGGTGTCG 
TTCAGCACTG TCCTCAAGCA ACCCTTCCGT TCCTCCTGTC ACTGCTGCCG GCCCCAGACT 
TCCAAGCTGA AGGCACTGCG GCTGCGATGC TCAGGGGGCA TGCGACTCAC TGCCACCTAC 
CGGTACATCC TCTCCTGTCA CTGCGAGGAA TGCAATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDP ( NM_000266.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000257.1 Homo sapiens NDP, norrin cystine knot growth factor (NDP), mRNA.

Price & Availability↑

CloneID	OHu26248
Clone ID Related Accession (Same CDS sequence)	NM_000266.4 , NM_000266.3
Accession Version	NM_000266.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	norrin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA714180.1, X65882.1 and BC029901.1. This sequence is a reference standard in the RefSeqGene project. On Feb 18, 2009 this sequence version replaced NM_000266.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X65724.1, SRR1660809.89329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAAAC ATGTACTAGC TGCATCCTTT TCTATGCTCT CCCTGCTGGT GATAATGGGA 
GATACAGACA GTAAAACGGA CAGCTCATTC ATAATGGACT CGGACCCTCG ACGCTGCATG 
AGGCACCACT ATGTGGATTC TATCAGTCAC CCATTGTACA AGTGTAGCTC AAAGATGGTG 
CTCCTGGCCA GGTGCGAGGG GCACTGCAGC CAGGCGTCAC GCTCCGAGCC TTTGGTGTCG 
TTCAGCACTG TCCTCAAGCA ACCCTTCCGT TCCTCCTGTC ACTGCTGCCG GCCCCAGACT 
TCCAAGCTGA AGGCACTGCG GCTGCGATGC TCAGGGGGCA TGCGACTCAC TGCCACCTAC 
CGGTACATCC TCTCCTGTCA CTGCGAGGAA TGCAATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000257.1
CDS	580..981
Translation	MRKHVLAASFSMLSLLVIMGDTDSKTDSSFIMDSDPRRCMRHHYVDSISHPLYKCSSKMVLLARCEGHCSQASRSEPLVSFSTVLKQPFRSSCHCCRPQTSKLKALRLRCSGGMRLTATYRYILSCHCEECNS

Target ORF information:

RefSeq Version	NM_000266.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NDP, norrin cystine knot growth factor (NDP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000266.3

ORF Insert Sequence:

ATGAGAAAAC ATGTACTAGC TGCATCCTTT TCTATGCTCT CCCTGCTGGT GATAATGGGA 
GATACAGACA GTAAAACGGA CAGCTCATTC ATAATGGACT CGGACCCTCG ACGCTGCATG 
AGGCACCACT ATGTGGATTC TATCAGTCAC CCATTGTACA AGTGTAGCTC AAAGATGGTG 
CTCCTGGCCA GGTGCGAGGG GCACTGCAGC CAGGCGTCAC GCTCCGAGCC TTTGGTGTCG 
TTCAGCACTG TCCTCAAGCA ACCCTTCCGT TCCTCCTGTC ACTGCTGCCG GCCCCAGACT 
TCCAAGCTGA AGGCACTGCG GCTGCGATGC TCAGGGGGCA TGCGACTCAC TGCCACCTAC 
CGGTACATCC TCTCCTGTCA CTGCGAGGAA TGCAATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Next-generation sequencing reveals a novel NDP gene mutation in a Chinese family with Norrie disease.
Indian journal of ophthalmology65(11)1161-1165(2017 Nov)
Huang X,Tian M,Li J,Cui L,Li M,Zhang J

Mutation Spectrum of the LRP5, NDP, and TSPAN12 Genes in Chinese Patients With Familial Exudative Vitreoretinopathy.
Investigative ophthalmology & visual science58(13)5949-5957(2017 Nov)
Tang M,Sun L,Hu A,Yuan M,Yang Y,Peng X,Ding X

Mutations in LRP5,FZD4, TSPAN12, NDP, ZNF408, or KIF11 Genes Account for 38.7% of Chinese Patients With Familial Exudative Vitreoretinopathy.
Investigative ophthalmology & visual science58(5)2623-2629(2017 May)
Rao FQ,Cai XB,Cheng FF,Cheng W,Fang XL,Li N,Huang XF,Li LH,Jin ZB