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POR cDNA ORF clone, Homo sapiens(Human)

Gene Symbol POR
Entrez Gene ID 5447
Full Name cytochrome p450 oxidoreductase
Synonyms CPR,CYPOR,P450R
General protein information
Preferred Names

cytochrome p450 oxidoreductase

Names

NADPH--cytochrome P450 reductase
NADPH-dependent cytochrome P450 reductase
P450 (cytochrome) oxidoreductase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q11.23

Summary This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:

124015

Disorder Html: Antley-Bixler syndrome with genital anomalies and disordered

mRNA and Protein(s)

mRNA Protein Name
NM_000941.2 NP_000932.3 NADPH--cytochrome P450 reductase



Canis lupus familiaris (dog) POR NP_001171276.1
Gallus gallus (chicken) POR NP_001182725.1
Kluyveromyces lactis KLLA0D08712g XP_453451.1
Homo sapiens (human) POR NP_000932.3
Pan troglodytes (chimpanzee) POR XP_001155755.2
Drosophila melanogaster (fruit fly) Cpr NP_001260128.1
Caenorhabditis elegans (roundworm) emb-8 NP_498103.1
Oryza sativa (rice) Os09g0558900 NP_001063914.1
Oryza sativa (rice) Os08g0243500 NP_001061346.1
Macaca mulatta (rhesus monkey) POR XP_001109664.1
Rattus norvegicus (Norway rat) Por NP_113764.1
Eremothecium gossypii AGOS_AAL121C NP_982421.1
Schizosaccharomyces pombe (fission yeast) ccr1 NP_596046.1
Magnaporthe oryzae (rice blast fungus) MGG_07117 XP_003715306.1
Danio rerio (zebrafish) por NP_001030154.1
Bos taurus (cattle) POR NP_001030467.1
Mus musculus (house mouse) Por NP_032924.1
Saccharomyces cerevisiae (baker's yeast) NCP1 NP_011908.1
Neurospora crassa NCU09741 XP_964443.1
Arabidopsis thaliana (thale cress) ATR2 NP_849472.2
Xenopus tropicalis (tropical clawed frog) por XP_002933864.1


Related articles in PubMed

Influence of genetic polymorphisms in cytochrome P450 oxidoreductase on the variability in stable warfarin maintenance dose in Han Chinese.
Zeng WT, Xu Q, Li CH, Chen WY, Sun XT, Wang X, Yang YY, Shi H, Yang ZS
European journal of clinical pharmacology72(11)1327-1334(2016 Nov)

The P450 oxidoreductase (POR) rs2868177 and cytochrome P450 (CYP) 2B6*6 polymorphisms contribute to the interindividual variability in human CYP2B6 activity.
Gao LC, Liu FQ, Yang L, Cheng L, Dai HY, Tao R, Cao SP, Wang D, Tang J
European journal of clinical pharmacology72(10)1205-1213(2016 Oct)

A Lack of Significant Effect of POR*28 Allelic Variant on Tacrolimus Exposure in Kidney Transplant Recipients.
Jannot AS, Vuillemin X, Etienne I, Buchler M, Hurault de Ligny B, Choukroun G, Colosio C, Thierry A, Vigneau C, Moulin B, Rerolle JP, Heng AE, Subra JF, Legendre C, Beaune P, Loriot MA, Thervet E, Pallet N
Therapeutic drug monitoring38(2)223-9(2016 Apr)

Delayed diagnosis of disorder of sex development (DSD) due to P450 oxidoreductase (POR) deficiency.
Koika V, Armeni AK, Georgopoulos NA
Hormones (Athens, Greece)15(2)277-82(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

To promote intracellular iron flux, an iron chaperone appears to be essential for receiving iron from heme catabolism. Data suggest that PCBP2 competes with CPR for binding HO1; PCBP2 K homology 3 domain is important for HO1/PCBP2 interaction; heme prompts HO1/CPR multimer and decreases HO1/PCBP2 multimer. [PCBP2 = poly(rC) binding protein 2; CPR = cytochrome P450 reductase; HO1 = heme oxidase 1]
Title: The iron chaperone poly(rC)-binding protein 2 forms a metabolon with the heme oxygenase 1/cytochrome P450 reductase complex for heme catabolism and iron transfer.

No significant differences in the distribution of haplotypes between cases and controls were detected. In conclusion, this study reveals that four SNPs in the POR gene (rs3823884, rs3898649, rs239953 and rs17685) may affect the susceptibility of smoking cessation in a Chinese Han population.
Title: Associations of cytochrome P450 oxidoreductase genetic polymorphisms with smoking cessation in a Chinese population.

For the first time, the SNP POR*37 831-35C>T was confirmed as a minor but statistically significant factor associated with inter-individual variation in warfarin maintenance dose in Han Chinese
Title: Influence of genetic polymorphisms in cytochrome P450 oxidoreductase on the variability in stable warfarin maintenance dose in Han Chinese.

Current studies demonstrate that, unlike previously described mutations, A287P causes POR deficiency disorder due to conformational instability leading to proteolytic susceptibility in vivo, rather than through an inherent flavin-binding defect.
Title: Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype.

analysis of the role of the active site loop in coenzyme binding and flavin reduction in cytochrome P450 reductase
Title: Role of active site loop in coenzyme binding and flavin reduction in cytochrome P450 reductase.

The following POR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25584 NM_000941.2
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Homo sapiens cytochrome p450 oxidoreductase (POR), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25584
Clone ID Related Accession (Same CDS sequence) NM_000941.2
Accession Version NM_000941.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-29
Organism Homo sapiens(Human)
Product NADPH--cytochrome P450 reductase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA701694.1, BP244929.1, BC034277.1, AF258341.1, BG104767.1 and AK129978.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XM_005250459.1, XM_005277600.1, NM_000941.1. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in higher primate species. This starting position is most commonly referred to in the literature, and the numbering system used to describe disease-associated mutations is based on this protein start. This includes data in the P450 oxidoreductase (POR) allele nomenclature locus-specific database, and the Human Gene Mutation Database (HGMD). This start codon is restricted to higher primate species, and it has a weak Kozak signal. However, it should be noted that an alternative downstream start codon, which has a strong Kozak signal and is much more widely conserved, is also present. The use of this downstream start codon would result in a protein that is 3 aa shorter at the N-terminus. Protein sequencing in PMID:2513880 indicates that this is the preferred start codon in vivo. It is therefore possible that the ribosome will initiate at the upstream start codon only a small fraction of the time (if at all), while leaky scanning will allow the downstream start codon to be used predominantly. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.67197.1, SRR1803615.192339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGATCAACA TGGGAGACTC CCACGTGGAC ACCAGCTCCA CCGTGTCCGA GGCGGTGGCC 
GAAGAAGTAT CTCTTTTCAG CATGACGGAC ATGATTCTGT TTTCGCTCAT CGTGGGTCTC
CTAACCTACT GGTTCCTCTT CAGAAAGAAA AAAGAAGAAG TCCCCGAGTT CACCAAAATT
CAGACATTGA CCTCCTCTGT CAGAGAGAGC AGCTTTGTGG AAAAGATGAA GAAAACGGGG
AGGAACATCA TCGTGTTCTA CGGCTCCCAG ACGGGGACTG CAGAGGAGTT TGCCAACCGC
CTGTCCAAGG ACGCCCACCG CTACGGGATG CGAGGCATGT CAGCGGACCC TGAGGAGTAT
GACCTGGCCG ACCTGAGCAG CCTGCCAGAG ATCGACAACG CCCTGGTGGT TTTCTGCATG
GCCACCTACG GTGAGGGAGA CCCCACCGAC AATGCCCAGG ACTTCTACGA CTGGCTGCAG
GAGACAGACG TGGATCTCTC TGGGGTCAAG TTCGCGGTGT TTGGTCTTGG GAACAAGACC
TACGAGCACT TCAATGCCAT GGGCAAGTAC GTGGACAAGC GGCTGGAGCA GCTCGGCGCC
CAGCGCATCT TTGAGCTGGG GTTGGGCGAC GACGATGGGA ACTTGGAGGA GGACTTCATC
ACCTGGCGAG AGCAGTTCTG GCCGGCCGTG TGTGAACACT TTGGGGTGGA AGCCACTGGC
GAGGAGTCCA GCATTCGCCA GTACGAGCTT GTGGTCCACA CCGACATAGA TGCGGCCAAG
GTGTACATGG GGGAGATGGG CCGGCTGAAG AGCTACGAGA ACCAGAAGCC CCCCTTTGAT
GCCAAGAATC CGTTCCTGGC TGCAGTCACC ACCAACCGGA AGCTGAACCA GGGAACCGAG
CGCCACCTCA TGCACCTGGA ATTGGACATC TCGGACTCCA AAATCAGGTA TGAATCTGGG
GACCACGTGG CTGTGTACCC AGCCAACGAC TCTGCTCTCG TCAACCAGCT GGGCAAAATC
CTGGGTGCCG ACCTGGACGT CGTCATGTCC CTGAACAACC TGGATGAGGA GTCCAACAAG
AAGCACCCAT TCCCGTGCCC TACGTCCTAC CGCACGGCCC TCACCTACTA CCTGGACATC
ACCAACCCGC CGCGTACCAA CGTGCTGTAC GAGCTGGCGC AGTACGCCTC GGAGCCCTCG
GAGCAGGAGC TGCTGCGCAA GATGGCCTCC TCCTCCGGCG AGGGCAAGGA GCTGTACCTG
AGCTGGGTGG TGGAGGCCCG GAGGCACATC CTGGCCATCC TGCAGGACTG CCCGTCCCTG
CGGCCCCCCA TCGACCACCT GTGTGAGCTG CTGCCGCGCC TGCAGGCCCG CTACTACTCC
ATCGCCTCAT CCTCCAAGGT CCACCCCAAC TCTGTGCACA TCTGTGCGGT GGTTGTGGAG
TACGAGACCA AGGCTGGCCG CATCAACAAG GGCGTGGCCA CCAACTGGCT GCGGGCCAAG
GAGCCTGCCG GGGAGAACGG CGGCCGTGCG CTGGTGCCCA TGTTCGTGCG CAAGTCCCAG
TTCCGCCTGC CCTTCAAGGC CACCACGCCT GTCATCATGG TGGGCCCCGG CACCGGGGTG
GCACCCTTCA TAGGCTTCAT CCAGGAGCGG GCCTGGCTGC GACAGCAGGG CAAGGAGGTG
GGGGAGACGC TGCTGTACTA CGGCTGCCGC CGCTCGGATG AGGACTACCT GTACCGGGAG
GAGCTGGCGC AGTTCCACAG GGACGGTGCG CTCACCCAGC TCAACGTGGC CTTCTCCCGG
GAGCAGTCCC ACAAGGTCTA CGTCCAGCAC CTGCTAAAGC AAGACCGAGA GCACCTGTGG
AAGTTGATCG AAGGCGGTGC CCACATCTAC GTCTGTGGGG ATGCACGGAA CATGGCCAGG
GATGTGCAGA ACACCTTCTA CGACATCGTG GCTGAGCTCG GGGCCATGGA GCACGCGCAG
GCGGTGGACT ACATCAAGAA ACTGATGACC AAGGGCCGCT ACTCCCTGGA CGTGTGGAGC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000932.3
CDS83..2125
Misc Feature(1)245..2119
Misc Feature(2)335..748
Misc Feature(3)920..2119
Misc Feature(4)order(983..985,1511..1513,1517..1519,1691..1696,1787..1792,1877..1882,1895..1897,1901..1903,1907..1909,1982..1984,1994..1996,2003..2005)
Misc Feature(5)order(1046..1048,1451..1462,1505..1513,1553..1564,2117..2119)
Misc Feature(6)order(1451..1453,1457..1462)
Misc Feature(7)order(1460..1462,1976..1978,2111..2113,2117..2119)
Misc Feature(8)order(1553..1555,1562..1564,1571..1573)
Misc Feature(9)order(1679..1681,1691..1702,1706..1708)
Exon (1)1..78
Exon (2)79..270
Exon (3)271..319
Exon (4)320..448
Exon (5)449..598
Exon (6)599..723
Exon (7)724..813
Exon (8)814..912
Exon (9)913..1029
Exon (10)1030..1148
Exon (11)1149..1330
Exon (12)1331..1480
Exon (13)1481..1751
Exon (14)1752..1897
Exon (15)1898..1980
Exon (16)1981..2499
Translation

Target ORF information:

RefSeq Version NM_000941.2
Organism Homo sapiens(Human)
Definition Homo sapiens cytochrome p450 oxidoreductase (POR), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000941.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGATCAACA TGGGAGACTC CCACGTGGAC ACCAGCTCCA CCGTGTCCGA GGCGGTGGCC 
GAAGAAGTAT CTCTTTTCAG CATGACGGAC ATGATTCTGT TTTCGCTCAT CGTGGGTCTC
CTAACCTACT GGTTCCTCTT CAGAAAGAAA AAAGAAGAAG TCCCCGAGTT CACCAAAATT
CAGACATTGA CCTCCTCTGT CAGAGAGAGC AGCTTTGTGG AAAAGATGAA GAAAACGGGG
AGGAACATCA TCGTGTTCTA CGGCTCCCAG ACGGGGACTG CAGAGGAGTT TGCCAACCGC
CTGTCCAAGG ACGCCCACCG CTACGGGATG CGAGGCATGT CAGCGGACCC TGAGGAGTAT
GACCTGGCCG ACCTGAGCAG CCTGCCAGAG ATCGACAACG CCCTGGTGGT TTTCTGCATG
GCCACCTACG GTGAGGGAGA CCCCACCGAC AATGCCCAGG ACTTCTACGA CTGGCTGCAG
GAGACAGACG TGGATCTCTC TGGGGTCAAG TTCGCGGTGT TTGGTCTTGG GAACAAGACC
TACGAGCACT TCAATGCCAT GGGCAAGTAC GTGGACAAGC GGCTGGAGCA GCTCGGCGCC
CAGCGCATCT TTGAGCTGGG GTTGGGCGAC GACGATGGGA ACTTGGAGGA GGACTTCATC
ACCTGGCGAG AGCAGTTCTG GCCGGCCGTG TGTGAACACT TTGGGGTGGA AGCCACTGGC
GAGGAGTCCA GCATTCGCCA GTACGAGCTT GTGGTCCACA CCGACATAGA TGCGGCCAAG
GTGTACATGG GGGAGATGGG CCGGCTGAAG AGCTACGAGA ACCAGAAGCC CCCCTTTGAT
GCCAAGAATC CGTTCCTGGC TGCAGTCACC ACCAACCGGA AGCTGAACCA GGGAACCGAG
CGCCACCTCA TGCACCTGGA ATTGGACATC TCGGACTCCA AAATCAGGTA TGAATCTGGG
GACCACGTGG CTGTGTACCC AGCCAACGAC TCTGCTCTCG TCAACCAGCT GGGCAAAATC
CTGGGTGCCG ACCTGGACGT CGTCATGTCC CTGAACAACC TGGATGAGGA GTCCAACAAG
AAGCACCCAT TCCCGTGCCC TACGTCCTAC CGCACGGCCC TCACCTACTA CCTGGACATC
ACCAACCCGC CGCGTACCAA CGTGCTGTAC GAGCTGGCGC AGTACGCCTC GGAGCCCTCG
GAGCAGGAGC TGCTGCGCAA GATGGCCTCC TCCTCCGGCG AGGGCAAGGA GCTGTACCTG
AGCTGGGTGG TGGAGGCCCG GAGGCACATC CTGGCCATCC TGCAGGACTG CCCGTCCCTG
CGGCCCCCCA TCGACCACCT GTGTGAGCTG CTGCCGCGCC TGCAGGCCCG CTACTACTCC
ATCGCCTCAT CCTCCAAGGT CCACCCCAAC TCTGTGCACA TCTGTGCGGT GGTTGTGGAG
TACGAGACCA AGGCTGGCCG CATCAACAAG GGCGTGGCCA CCAACTGGCT GCGGGCCAAG
GAGCCTGCCG GGGAGAACGG CGGCCGTGCG CTGGTGCCCA TGTTCGTGCG CAAGTCCCAG
TTCCGCCTGC CCTTCAAGGC CACCACGCCT GTCATCATGG TGGGCCCCGG CACCGGGGTG
GCACCCTTCA TAGGCTTCAT CCAGGAGCGG GCCTGGCTGC GACAGCAGGG CAAGGAGGTG
GGGGAGACGC TGCTGTACTA CGGCTGCCGC CGCTCGGATG AGGACTACCT GTACCGGGAG
GAGCTGGCGC AGTTCCACAG GGACGGTGCG CTCACCCAGC TCAACGTGGC CTTCTCCCGG
GAGCAGTCCC ACAAGGTCTA CGTCCAGCAC CTGCTAAAGC AAGACCGAGA GCACCTGTGG
AAGTTGATCG AAGGCGGTGC CCACATCTAC GTCTGTGGGG ATGCACGGAA CATGGCCAGG
GATGTGCAGA ACACCTTCTA CGACATCGTG GCTGAGCTCG GGGCCATGGA GCACGCGCAG
GCGGTGGACT ACATCAAGAA ACTGATGACC AAGGGCCGCT ACTCCCTGGA CGTGTGGAGC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Influence of genetic polymorphisms in cytochrome P450 oxidoreductase on the variability in stable warfarin maintenance dose in Han Chinese.
European journal of clinical pharmacology72(11)1327-1334(2016 Nov)
Zeng WT,Xu Q,Li CH,Chen WY,Sun XT,Wang X,Yang YY,Shi H,Yang ZS


book

The P450 oxidoreductase (POR) rs2868177 and cytochrome P450 (CYP) 2B6*6 polymorphisms contribute to the interindividual variability in human CYP2B6 activity.
European journal of clinical pharmacology72(10)1205-1213(2016 Oct)
Gao LC,Liu FQ,Yang L,Cheng L,Dai HY,Tao R,Cao SP,Wang D,Tang J


book

A Lack of Significant Effect of POR*28 Allelic Variant on Tacrolimus Exposure in Kidney Transplant Recipients.
Therapeutic drug monitoring38(2)223-9(2016 Apr)
Jannot AS,Vuillemin X,Etienne I,Buchler M,Hurault de Ligny B,Choukroun G,Colosio C,Thierry A,Vigneau C,Moulin B,Rerolle JP,Heng AE,Subra JF,Legendre C,Beaune P,Loriot MA,Thervet E,Pallet N


book

Delayed diagnosis of disorder of sex development (DSD) due to P450 oxidoreductase (POR) deficiency.
Hormones (Athens, Greece)15(2)277-82(2016 Apr)
Koika V,Armeni AK,Georgopoulos NA


 
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