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SDHAF2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SDHAF2
Entrez Gene ID 54949
Full Name succinate dehydrogenase complex assembly factor 2
Synonyms C11orf79,PGL2,SDH5
General protein information
Preferred Names

succinate dehydrogenase complex assembly factor 2

Names

succinate dehydrogenase assembly factor 2, mitochondrial
SDH assembly factor 2
hSDH5
succinate dehydrogenase subunit 5, mitochondrial

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q12.2

Disorder MIM:

613019

Disorder Html: Paragangliomas 2, 601650 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_017841.2 NP_060311.1 succinate dehydrogenase assembly factor 2, mitochondrial



Rattus norvegicus (Norway rat) Sdhaf2 NP_001008372.1
Drosophila melanogaster (fruit fly) CG14757 NP_610363.1
Drosophila melanogaster (fruit fly) CG12895 NP_001260868.1
Homo sapiens (human) SDHAF2 NP_060311.1
Eremothecium gossypii AGOS_AER200C NP_985057.1
Neurospora crassa NCU01343 XP_961429.1
Danio rerio (zebrafish) sdhaf2 NP_001076333.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012191 XP_320341.4
Kluyveromyces lactis KLLA0E13333g XP_454550.1
Pan troglodytes (chimpanzee) PPP1R32 NP_001238783.1
Canis lupus familiaris (dog) SDHAF2 XP_533273.2
Mus musculus (house mouse) Sdhaf2 NP_079609.2
Saccharomyces cerevisiae (baker's yeast) EMI5 NP_014570.1
Xenopus tropicalis (tropical clawed frog) sdhaf2 XP_002937676.1
Macaca mulatta (rhesus monkey) SDHAF2 XP_001082359.2
Bos taurus (cattle) SDHAF2 NP_001070513.1
Gallus gallus (chicken) LOC426015 NP_001264818.1
Magnaporthe oryzae (rice blast fungus) MGG_07034 XP_003715216.1


Related articles in PubMed

A two-decade experience of head and neck paragangliomas in a whole population-based single centre cohort.
Anttila T, Häyry V, Nicoli T, Hagström J, Aittomäki K, Vikatmaa P, Niemelä M, Saarilahti K, Mäkitie A, Bäck LJ
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(8)2045-53(2015 Aug)

Universal genetic screening uncovers a novel presentation of an SDHAF2 mutation.
Casey R, Garrahy A, Tuthill A, O'Halloran D, Joyce C, Casey MB, O'Shea P, Bell M
The Journal of clinical endocrinology and metabolism99(7)E1392-6(2014 Jul)

Mitochondrial matrix proteostasis is linked to hereditary paraganglioma: LON-mediated turnover of the human flavinylation factor SDH5 is regulated by its interaction with SDHA.
Bezawork-Geleta A, Saiyed T, Dougan DA, Truscott KN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(4)1794-804(2014 Apr)

Role of SDHAF2 and SDHD in von Hippel-Lindau associated pheochromocytomas.
Kugelberg J, Welander J, Schiavi F, Fassina A, Bäckdahl M, Larsson C, Opocher G, Söderkvist P, Dahia PL, Neumann HP, Gimm O
World journal of surgery38(3)724-32(2014 Mar)

Succinate dehydrogenase 5 (SDH5) regulates glycogen synthase kinase 3β-β-catenin-mediated lung cancer metastasis.
Liu J, Gao L, Zhang H, Wang D, Wang M, Zhu J, Pang C, Wang C
The Journal of biological chemistry288(41)29965-73(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data show that SDHA flavination is independent of SDHAF2 in breast cancer cells, employing an alternative mechanism.
Title: The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells.

FAD interacts noncovalently with SDHA in the absence of SDH5
Title: In-vitro, SDH5-dependent flavinylation of immobilized human respiratory complex II flavoprotein.

Autosomal dominant susceptibility for Paraganglioma is modified by imprinting and mutations in the SDHAF2 gene cause Paragangliomas only when the mutation is inherited from father.
Title: A two-decade experience of head and neck paragangliomas in a whole population-based single centre cohort.

New mutation found in SDHAF2 gene in pheochromocytoma/paraganglioma patients.
Title: Universal genetic screening uncovers a novel presentation of an SDHAF2 mutation.

Data indicate that SDH5 is protected from mitochondrial LON (LONM)-mediated degradation in mitochondria by its stable interaction with SDHA, a state that is dysregulated in hereditary paraganglioma 2 (PGL2).
Title: Mitochondrial matrix proteostasis is linked to hereditary paraganglioma: LON-mediated turnover of the human flavinylation factor SDH5 is regulated by its interaction with SDHA.

The following SDHAF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHAF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22591 NM_017841.2
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Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22591
Clone ID Related Accession (Same CDS sequence) NM_017841.2
Accession Version NM_017841.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-02
Organism Homo sapiens(Human)
Product succinate dehydrogenase assembly factor 2, mitochondrial
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000494.1, AI039966.1 and AI660205.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_017841.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000494.1, BC002331.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060311.1
CDS23..523
Misc Feature(1)221..442
Exon (1)1..58
Exon (2)59..282
Exon (3)283..392
Exon (4)393..1219
Translation

Target ORF information:

RefSeq Version NM_017841.2
Organism Homo sapiens(Human)
Definition Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_017841.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A two-decade experience of head and neck paragangliomas in a whole population-based single centre cohort.
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(8)2045-53(2015 Aug)
Anttila T,Häyry V,Nicoli T,Hagström J,Aittomäki K,Vikatmaa P,Niemelä M,Saarilahti K,Mäkitie A,Bäck LJ


book

Universal genetic screening uncovers a novel presentation of an SDHAF2 mutation.
The Journal of clinical endocrinology and metabolism99(7)E1392-6(2014 Jul)
Casey R,Garrahy A,Tuthill A,O'Halloran D,Joyce C,Casey MB,O'Shea P,Bell M


book

Mitochondrial matrix proteostasis is linked to hereditary paraganglioma: LON-mediated turnover of the human flavinylation factor SDH5 is regulated by its interaction with SDHA.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(4)1794-804(2014 Apr)
Bezawork-Geleta A,Saiyed T,Dougan DA,Truscott KN


book

Role of SDHAF2 and SDHD in von Hippel-Lindau associated pheochromocytomas.
World journal of surgery38(3)724-32(2014 Mar)
Kugelberg J,Welander J,Schiavi F,Fassina A,Bäckdahl M,Larsson C,Opocher G,Söderkvist P,Dahia PL,Neumann HP,Gimm O


book

Succinate dehydrogenase 5 (SDH5) regulates glycogen synthase kinase 3β-β-catenin-mediated lung cancer metastasis.
The Journal of biological chemistry288(41)29965-73(2013 Oct)
Liu J,Gao L,Zhang H,Wang D,Wang M,Zhu J,Pang C,Wang C


 
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