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AP5S1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol AP5S1
Entrez Gene ID 55317
Full Name adaptor related protein complex 5 sigma 1 subunit
Synonyms C20orf29
General protein information
Preferred Names

adaptor related protein complex 5 sigma 1 subunit

Names

AP-5 complex subunit sigma-1
adapter-related protein complex 5 sigma subunit
adaptor-related protein complex 5 sigma subunit
protein C20orf29
sigma5
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

20

20p13
Disorder MIM:

614824


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001204446.1 NP_001191375.1 AP-5 complex subunit sigma-1
NM_001204447.1 NP_001191376.1 AP-5 complex subunit sigma-1
NM_018347.2 NP_060817.1 AP-5 complex subunit sigma-1
NM_018347.3 NP_060817.1 AP-5 complex subunit sigma-1
NM_001204447.2 NP_001191376.1 AP-5 complex subunit sigma-1

Pathways from BioSystems


Homologies


Homo sapiens (human) AP5S1 NP_001191375.1
C20orf29 XP_002798238.1
Mus musculus (house mouse) Ap5s1 NP_081405.2
Canis lupus familiaris (dog) AP5S1 XP_851396.1
Rattus norvegicus (Norway rat) Ap5s1 NP_001128090.1
Xenopus tropicalis (tropical clawed frog) ap5s1 NP_001107332.1
Pan troglodytes (chimpanzee) AP5S1 XP_001163166.1
Bos taurus (cattle) AP5S1 NP_001029983.1
Danio rerio (zebrafish) ap5s1 XP_005156643.1

Gene Ontology


Bibliography

Related articles in PubMed

The fifth adaptor protein complex.
Hirst J, Barlow LD, Francisco GC, Sahlender DA, Seaman MN, Dacks JB, Robinson MS
PLoS biology9(10)e1001170(2011 Oct)

A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.
S?abicki M, Theis M, Krastev DB, Samsonov S, Mundwiller E, Junqueira M, Paszkowski-Rogacz M, Teyra J, Heninger AK, Poser I, Prieur F, Truchetto J, Confavreux C, Marelli C, Durr A, Camdessanche JP, Brice A, Shevchenko A, Pisabarro MT, Stevanin G, Buchholz F
PLoS biology8(6)e1000408(2010 Jun)

The DNA sequence and comparative analysis of human chromosome 20.
Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J
Nature414(6866)865-71(2001 Dec 20-27)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AP5S1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP5S1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu32238 NM_001204447.2
Latest version!		 Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu32238 NM_018347.3
Latest version!		 Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu32238 NM_001204446.1
Latest version!		 Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
View Old Accession Versions >>
OHu32238 NM_018347.2 Homo sapiens adaptor related protein complex 5 sigma 1 subunit (AP5S1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu32238 NM_001204447.1 Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu32238
Clone ID Related Accession (Same CDS sequence) NM_001204446.1 , NM_001204447.1 , NM_018347.2 , NM_018347.3 , NM_001204447.2
Accession Version NM_001204446.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 603bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product AP-5 complex subunit sigma-1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA253969.1, DA129391.1 and AK002030.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: DA129391.1, DA763550.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191375.1
CDS348..950
Translation

Target ORF information:

RefSeq Version NM_001204446.1
Organism Homo sapiens(human)
Definition Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001204446.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32238
Clone ID Related Accession (Same CDS sequence) NM_001204446.1 , NM_001204447.1 , NM_018347.2 , NM_018347.3 , NM_001204447.2
Accession Version NM_001204447.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 603bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product AP-5 complex subunit sigma-1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA253969.1, AK093332.1, DB190907.1 and AI338486.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK093332.1, SRR1163657.50623.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191376.1
CDS196..798
Translation

Target ORF information:

RefSeq Version NM_001204447.1
Organism Homo sapiens(human)
Definition Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001204447.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32238
Clone ID Related Accession (Same CDS sequence) NM_001204446.1 , NM_001204447.1 , NM_018347.2 , NM_018347.3 , NM_001204447.2
Accession Version NM_018347.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 603bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product AP-5 complex subunit sigma-1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA253969.1, BC043344.1 and AK002030.1. On Mar 2, 2011 this sequence version replaced NM_018347.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK002030.1, SRR1660809.186259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060817.1
CDS252..854
Translation

Target ORF information:

RefSeq Version NM_018347.2
Organism Homo sapiens(human)
Definition Homo sapiens adaptor related protein complex 5 sigma 1 subunit (AP5S1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018347.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32238
Clone ID Related Accession (Same CDS sequence) NM_001204446.1 , NM_001204447.1 , NM_018347.2 , NM_018347.3 , NM_001204447.2
Accession Version NM_018347.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 603bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-01
Organism Homo sapiens(human)
Product AP-5 complex subunit sigma-1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA253969.1, BC043344.1, AK002030.1 and AL109804.41. On Nov 24, 2018 this sequence version replaced NM_018347.2. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.400910.1, AK002030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000615891.2/ ENSP00000481750.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060817.1
CDS229..831
Translation

Target ORF information:

RefSeq Version NM_018347.3
Organism Homo sapiens(human)
Definition Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018347.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu32238
Clone ID Related Accession (Same CDS sequence) NM_001204446.1 , NM_001204447.1 , NM_018347.2 , NM_018347.3 , NM_001204447.2
Accession Version NM_001204447.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 603bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-30
Organism Homo sapiens(human)
Product AP-5 complex subunit sigma-1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA253969.1, AK093332.1, DB190907.1, AI338486.1 and AL109804.41. On May 31, 2019 this sequence version replaced NM_001204447.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.52990.1, AK093332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191376.1
CDS173..775
Translation

Target ORF information:

RefSeq Version NM_001204447.2
Organism Homo sapiens(human)
Definition Homo sapiens adaptor related protein complex 5 subunit sigma 1 (AP5S1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001204447.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTCCACG CCTTCCTCAT TCACACCTTG AGGGCCCCGA ATACTGAGGA CACGGGCCTT 
TGCCGAGTGC TGTACTCCTG CGTCTTCGGT GCTGAGAAGT CACCTGATGA CCCACGGCCG 
CATGGTGCCG AGAGGGACAG GCTTCTCCGG AAGGAACAGA TTTTAGCTGT GGCCAGGCAG 
GTAGAGTCAA TGTGTCGGCT GCAGCAGCAG GCATCTGGCC GGCCCCCCAT GGACCTGCAG 
CCGCAATCCT CAGATGAGCA AGTGCCGCTG CACGAGGCCC CACGTGGGGC TTTCCGCCTG 
GCAGCAGAGA ACCCTTTCCA GGAGCCACGG ACGGTGGTGT GGCTGGGCGT GCTCTCGTTA 
GGCTTTGCCC TGGTGCTGGA TGCCCATGAG AACCTGCTAC TGGCTGAGGG CACGCTCCGG 
CTGCTGACAC GCCTCCTCCT TGACCACCTC CGGCTGCTGG CGCCCAGCAC CAGCCTTCTG 
CTGCGGGCTG ACCGCATTGA GGGCATCCTC ACCCGCTTCC TGCCACATGG TCAGCTGCTT 
TTCCTCAACG ACCAGTTTGT CCAAGGCCTG GAGAAGGAAT TCAGTGCCGC TTGGCCCCGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The fifth adaptor protein complex.
PLoS biology9(10)e1001170(2011 Oct)
Hirst J,Barlow LD,Francisco GC,Sahlender DA,Seaman MN,Dacks JB,Robinson MS


book

A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.
PLoS biology8(6)e1000408(2010 Jun)
S?abicki M,Theis M,Krastev DB,Samsonov S,Mundwiller E,Junqueira M,Paszkowski-Rogacz M,Teyra J,Heninger AK,Poser I,Prieur F,Truchetto J,Confavreux C,Marelli C,Durr A,Camdessanche JP,Brice A,Shevchenko A,Pisabarro MT,Stevanin G,Buchholz F


book

The DNA sequence and comparative analysis of human chromosome 20.
Nature414(6866)865-71(2001 Dec 20-27)
Deloukas P,Matthews LH,Ashurst J,Burton J,Gilbert JG,Jones M,Stavrides G,Almeida JP,Babbage AK,Bagguley CL,Bailey J,Barlow KF,Bates KN,Beard LM,Beare DM,Beasley OP,Bird CP,Blakey SE,Bridgeman AM,Brown AJ,Buck D,Burrill W,Butler AP,Carder C,Carter NP,Chapman JC,Clamp M,Clark G,Clark LN,Clark SY,Clee CM,Clegg S,Cobley VE,Collier RE,Connor R,Corby NR,Coulson A,Coville GJ,Deadman R,Dhami P,Dunn M,Ellington AG,Frankland JA,Fraser A,French L,Garner P,Grafham DV,Griffiths C,Griffiths MN,Gwilliam R,Hall RE,Hammond S,Harley JL,Heath PD,Ho S,Holden JL,Howden PJ,Huckle E,Hunt AR,Hunt SE,Jekosch K,Johnson CM,Johnson D,Kay MP,Kimberley AM,King A,Knights A,Laird GK,Lawlor S,Lehvaslaiho MH,Leversha M,Lloyd C,Lloyd DM,Lovell JD,Marsh VL,Martin SL,McConnachie LJ,McLay K,McMurray AA,Milne S,Mistry D,Moore MJ,Mullikin JC,Nickerson T,Oliver K,Parker A,Patel R,Pearce TA,Peck AI,Phillimore BJ,Prathalingam SR,Plumb RW,Ramsay H,Rice CM,Ross MT,Scott CE,Sehra HK,Shownkeen R,Sims S,Skuce CD,Smith ML,Soderlund C,Steward CA,Sulston JE,Swann M,Sycamore N,Taylor R,Tee L,Thomas DW,Thorpe A,Tracey A,Tromans AC,Vaudin M,Wall M,Wallis JM,Whitehead SL,Whittaker P,Willey DL,Williams L,Williams SA,Wilming L,Wray PW,Hubbard T,Durbin RM,Bentley DR,Beck S,Rogers J