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PRF1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PRF1
Entrez Gene ID 5551
Full Name perforin 1
Synonyms HPLH2,P1,PFP
General protein information
Preferred Names

perforin 1

Names

perforin-1
cytolysin
lymphocyte pore-forming protein
perforin 1 (pore forming protein)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

10

10q22.1

Summary The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008].
Disorder MIM:

170280

Disorder Html: Hemophagocytic lymphohistiocytosis, familial, 2, 603553 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001083116.1 NP_001076585.1 perforin-1 precursor
NM_005041.4 NP_005032.2 perforin-1 precursor



Homo sapiens (human) PRF1 NP_001076585.1
Xenopus tropicalis (tropical clawed frog) LOC100489175 XP_002942088.2
Xenopus tropicalis (tropical clawed frog) LOC101732838 XP_004919588.1
Xenopus tropicalis (tropical clawed frog) LOC100489324 XP_002942089.2
Xenopus tropicalis (tropical clawed frog) LOC100492973 XP_002941527.2
Xenopus tropicalis (tropical clawed frog) LOC101732506 XP_004918449.1
Xenopus tropicalis (tropical clawed frog) LOC100493300 XP_002941529.2
Danio rerio (zebrafish) prf1.7 XP_003200648.1
Danio rerio (zebrafish) LOC100000903 XP_001341008.1
Xenopus tropicalis (tropical clawed frog) LOC100489487 XP_002942090.2
Macaca mulatta (rhesus monkey) PRF1 XP_001107909.1
Pan troglodytes (chimpanzee) PRF1 XP_507837.2
Rattus norvegicus (Norway rat) Prf1 NP_059026.2
Gallus gallus (chicken) LOC101750363 XP_004945747.1
Danio rerio (zebrafish) LOC569443 XP_697920.2
Xenopus tropicalis (tropical clawed frog) LOC100491518 XP_002942100.2
Canis lupus familiaris (dog) PRF1 NP_001184111.1
Bos taurus (cattle) PRF1 NP_001137207.1
Mus musculus (house mouse) Prf1 NP_035203.3
Xenopus tropicalis (tropical clawed frog) LOC100493140 XP_002941528.1


Related articles in PubMed

Recurrent macrophage activation syndrome in spondyloarthritis and monoallelic missense mutations in PRF1: a description of one paediatric case.
Filocamo G, Petaccia A, Torcoletti M, Sieni E, Ravelli A, Corona F
Clinical and experimental rheumatology34(4)719(2016 Jul-Aug)

Search for the potential "second-hit" mechanism underlying the onset of familial hemophagocytic lymphohistiocytosis type 2 by whole-exome sequencing analysis.
Gao L, Dang X, Huang L, Zhu L, Fang M, Zhang J, Xu X, Zhu L, Li T, Zhao L, Wei J, Zhou J
Translational research : the journal of laboratory and clinical medicine17026-39(2016 Apr)

Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites.
Dotiwala F, Mulik S, Polidoro RB, Ansara JA, Burleigh BA, Walch M, Gazzinelli RT, Lieberman J
Nature medicine22(2)210-6(2016 Feb)

Accuracy of flow cytometric perforin screening for detecting patients with FHL due to PRF1 mutations.
Abdalgani M, Filipovich AH, Choo S, Zhang K, Gifford C, Villanueva J, Bleesing JJ, Marsh RA
Blood126(15)1858-60(2015 Oct)

Expression and Localization of Granzymes and Perforin in Human Calcific Aortic Valve Disease.
Ohukainen P, Näpäkangas J, Ohtonen P, Ruskoaho H, Taskinen P, Peltonen T, Rysä J
The Journal of heart valve disease24(5)612-20(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation in PRF1 gene is associated with hemophagocytic lymphohistiocytosis.
Title: Clinical presentation and outcome of pediatric patients with hemophagocytic lymphohistiocytosis in China: A retrospective multicenter study.

ITP patients displayed an increased frequency of rare missense variations of the PRF1 gene
Title: Decreased function of Fas and variations of the perforin gene in adult patients with primary immune thrombocytopenia.

Increased TIM3+CD8+T cells with lower perforin and granzyme B expression and higher CD95 expression in MDS patients were observed.
Title: Increased TIM3+CD8+T cells in Myelodysplastic Syndrome patients displayed less perforin and granzyme B secretion and higher CD95 expression.

PRF delivers granulysin and granzymes into parasite infected cells to kill the parasite.
Title: Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites.

Calcific aortic valve disease is characterized by increased expression of granzymes and perforin.
Title: Expression and Localization of Granzymes and Perforin in Human Calcific Aortic Valve Disease.

The following PRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23295 NM_001083116.1
Latest version!
Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu23295 NM_005041.4
Latest version!
Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00

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2-4 Clone 40% OFF
5 or more Clone 50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23295
Clone ID Related Accession (Same CDS sequence) NM_001083116.1 , NM_005041.4
Accession Version NM_001083116.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product perforin-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA941244.1, X13224.1, BC047695.1 and BQ654088.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2155751, SAMN01820691 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001076585.1
CDS162..1829
Misc Feature(1)105..107
Misc Feature(2)660..1268
Misc Feature(3)801..803
Misc Feature(4)1191..1193
Misc Feature(5)1323..1703
Misc Feature(6)order(1449..1451,1467..1469,1611..1613,1617..1619,1635..1637)
Misc Feature(7)774..782
Exon (1)1..131
Exon (2)132..700
Exon (3)701..2534
Translation

Target ORF information:

RefSeq Version NM_001083116.1
Organism Homo sapiens(Human)
Definition Homo sapiens perforin 1 (PRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001083116.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23295
Clone ID Related Accession (Same CDS sequence) NM_001083116.1 , NM_005041.4
Accession Version NM_005041.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-29
Organism Homo sapiens(Human)
Product perforin-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816891.1, X13224.1, BC047695.1 and BQ654088.1. On Mar 21, 2007 this sequence version replaced NM_005041.3. Transcript Variant: This variant (1) represents the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ555237.1, SRR1163657.274701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005032.2
CDS136..1803
Misc Feature(1)82..84
Misc Feature(2)634..1242
Misc Feature(3)775..777
Misc Feature(4)1165..1167
Misc Feature(5)1297..1677
Misc Feature(6)order(1423..1425,1441..1443,1585..1587,1591..1593,1609..1611)
Misc Feature(7)748..756
Exon (1)1..131
Exon (2)132..674
Exon (3)675..2508
Translation

Target ORF information:

RefSeq Version NM_005041.4
Organism Homo sapiens(Human)
Definition Homo sapiens perforin 1 (PRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005041.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Recurrent macrophage activation syndrome in spondyloarthritis and monoallelic missense mutations in PRF1: a description of one paediatric case.
Clinical and experimental rheumatology34(4)719(2016 Jul-Aug)
Filocamo G,Petaccia A,Torcoletti M,Sieni E,Ravelli A,Corona F


book

Search for the potential "second-hit" mechanism underlying the onset of familial hemophagocytic lymphohistiocytosis type 2 by whole-exome sequencing analysis.
Translational research : the journal of laboratory and clinical medicine17026-39(2016 Apr)
Gao L,Dang X,Huang L,Zhu L,Fang M,Zhang J,Xu X,Zhu L,Li T,Zhao L,Wei J,Zhou J


book

Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites.
Nature medicine22(2)210-6(2016 Feb)
Dotiwala F,Mulik S,Polidoro RB,Ansara JA,Burleigh BA,Walch M,Gazzinelli RT,Lieberman J


book

Accuracy of flow cytometric perforin screening for detecting patients with FHL due to PRF1 mutations.
Blood126(15)1858-60(2015 Oct)
Abdalgani M,Filipovich AH,Choo S,Zhang K,Gifford C,Villanueva J,Bleesing JJ,Marsh RA


book

Expression and Localization of Granzymes and Perforin in Human Calcific Aortic Valve Disease.
The Journal of heart valve disease24(5)612-20(2015 Sep)
Ohukainen P,Näpäkangas J,Ohtonen P,Ruskoaho H,Taskinen P,Peltonen T,Rysä J


 
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