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NGLY1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol NGLY1
Entrez Gene ID 55768
Full Name N-glycanase 1
Synonyms CDDG,CDG1V,PNG1,PNGase
General protein information
Preferred Names

N-glycanase 1

Names

peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
hPNGase
peptide:N-glycanase
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

3

3p24.2
Summary This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009].
Disorder MIM:

610661

Disorder Html: Congenital disorder of deglycosylation, 615273 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_018297.4 NP_060767.2 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 1
XM_011533945.2 XP_011532247.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X5
XM_017006839.2 XP_016862328.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X2
NM_018297.3 NP_060767.2 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 1
XM_005265316.1 XP_005265373.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X1
XM_005265317.1 XP_005265374.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X4
XM_017006839.1 XP_016862328.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X2
NM_001145293.1 NP_001138765.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 2
NM_001145294.1 NP_001138766.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 3
NM_001145295.1 NP_001138767.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 4
XM_011533944.1 XP_011532246.1 peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X3

Pathways from BioSystems


Homologies


Bos taurus (cattle) NGLY1 NP_001179716.1
Rattus norvegicus (Norway rat) Ngly1 NP_001014158.1
Neurospora crassa NCU00651 XP_965791.1
Mus musculus (house mouse) Ngly1 NP_067479.2
Danio rerio (zebrafish) ngly1 NP_001018437.1
Homo sapiens (human) NGLY1 NP_060767.2
NGLY1 XP_001092796.1
Canis lupus familiaris (dog) NGLY1 XP_003639867.1
Gallus gallus (chicken) NGLY1 NP_001026159.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007390 XP_308442.4
Caenorhabditis elegans (roundworm) png-1 NP_492913.1
Saccharomyces cerevisiae (baker's yeast) PNG1 NP_015229.1
Kluyveromyces lactis KLLA0E24223g XP_455043.1
Magnaporthe oryzae (rice blast fungus) MGG_03598 XP_003716307.1
Pan troglodytes (chimpanzee) NGLY1 XP_003309721.1
Schizosaccharomyces pombe (fission yeast) SPBC1709.14 NP_595447.1
Drosophila melanogaster (fruit fly) Pngl NP_724444.3
Eremothecium gossypii AGOS_ABR193W NP_983142.1
Xenopus tropicalis (tropical clawed frog) ngly1 XP_002932449.1

Gene Ontology


Bibliography

Related articles in PubMed

Mitochondrial function requires NGLY1.
Kong J, Peng M, Ostrovsky J, Kwon YJ, Oretsky O, McCormick EM, He M, Argon Y, Falk MJ
Mitochondrion386-16(2018 Jan)

Tissue-specific regulation of BMP signaling by
Galeone A, Han SY, Huang C, Hosomi A, Suzuki T, Jafar-Nejad H
eLife6(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Prospective phenotyping of NGLY1-CDDG, the first congenital disorder of deglycosylation.
Lam C, Ferreira C, Krasnewich D, Toro C, Latham L, Zein WM, Lehky T, Brewer C, Baker EH, Thurm A, Farmer CA, Rosenzweig SD, Lyons JJ, Schreiber JM, Gropman A, Lingala S, Ghany MG, Solomon B, Macnamara E, Davids M, Stratakis CA, Kimonis V, Gahl WA, Wolfe L
Genetics in medicine : official journal of the American College of Medical Genetics19(2)160-168(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our prospective phenotyping expands the clinical spectrum of NGLY1-CDDG, offers prognostic information, and provides baseline data for evaluating therapeutic interventions
Title: Prospective phenotyping of NGLY1-CDDG, the first congenital disorder of deglycosylation.

The patients with NGLY1 deficiency show developmental delay, seizures, peripheral neuropathy, abnormal liver function and alacrima.
Title: A congenital disorder of deglycosylation: Biochemical characterization of N-glycanase 1 deficiency in patient fibroblasts.

NGLY1 mutation causes neuromotor impairment, intellectual disability, and neuropathy
Title: NGLY1 mutation causes neuromotor impairment, intellectual disability, and neuropathy.

This review summarizes the research history of cytoplasmic PNGase.
Title: The cytoplasmic peptide:N-glycanase (Ngly1)-basic science encounters a human genetic disorder.

Data indicate that N-glycanase 1 (NGLY1) deficiency is a novel autosomal recessive disorder of the endoplasmic reticulum-associated degradation pathway associated with neurological dysfunction, abnormal tear production, and liver disease.
Title: Mutations in NGLY1 cause an inherited disorder of the endoplasmic reticulum-associated degradation pathway.

The following NGLY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGLY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu12091 NM_018297.4
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu18936 NM_001145294.1
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $259.50-$363.30
$519.00
OHu69736 XM_011533944.1
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $307.30
$439.00
OHu11077 NM_001145293.1
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu12482 NM_001145295.1
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu37371 XM_005265316.1
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu37372 XM_005265317.1
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu69737 XM_011533945.2
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu94383 XM_017006839.2
Latest version!		 Homo sapiens N-glycanase 1 (NGLY1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu12091 NM_018297.3 Homo sapiens N-glycanase 1 (NGLY1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu94383 XM_017006839.1 Homo sapiens N-glycanase 1 (NGLY1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu12091
Clone ID Related Accession (Same CDS sequence) NM_018297.4 , NM_018297.3
Accession Version NM_018297.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-29
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092798.3, BC007226.1 and BQ017148.1. On Nov 22, 2018 this sequence version replaced NM_018297.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.667160.1, SRR1660803.2103.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000280700.10/ ENSP00000280700.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GGTATATTTG 
GCCCGAAAGG AAGGATCATC TTTTGCTTAT ATTTCCTGGA AGTTTGAGTG TGGGTCAGTT 
GGCCTAAAAG TAGATAGCAT TTCTATTAGA ACAAGTAGTC AAACTTTTCA GACTGGAACA 
GTAGAATGGA AATTGCGATC TGATACAGCA CAAGTAGAAC TGACAGGCGA TAACAGTCTT 
CACTCCTATG CTGATTTTTC TGGTGCCACT GAAGTTATTT TGGAAGCAGA ATTAAGCAGA 
GGAGATGGTG ATGTCGCTTG GCAACACACC CAGCTGTTTA GACAAAGCTT AAATGACCAT 
GAAGAAAATT GTTTGGAGAT AATTATAAAA TTCAGTGACC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060767.2
CDS54..2018
Translation

Target ORF information:

RefSeq Version NM_018297.4
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018297.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GGTATATTTG 
GCCCGAAAGG AAGGATCATC TTTTGCTTAT ATTTCCTGGA AGTTTGAGTG TGGGTCAGTT 
GGCCTAAAAG TAGATAGCAT TTCTATTAGA ACAAGTAGTC AAACTTTTCA GACTGGAACA 
GTAGAATGGA AATTGCGATC TGATACAGCA CAAGTAGAAC TGACAGGCGA TAACAGTCTT 
CACTCCTATG CTGATTTTTC TGGTGCCACT GAAGTTATTT TGGAAGCAGA ATTAAGCAGA 
GGAGATGGTG ATGTCGCTTG GCAACACACC CAGCTGTTTA GACAAAGCTT AAATGACCAT 
GAAGAAAATT GTTTGGAGAT AATTATAAAA TTCAGTGACC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu12091
Clone ID Related Accession (Same CDS sequence) NM_018297.4 , NM_018297.3
Accession Version NM_018297.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-17
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092798.3, BC007226.1 and BQ017148.1. On Feb 21, 2009 this sequence version replaced NM_018297.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.2103.1, SRR1660807.78384.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GGTATATTTG 
GCCCGAAAGG AAGGATCATC TTTTGCTTAT ATTTCCTGGA AGTTTGAGTG TGGGTCAGTT 
GGCCTAAAAG TAGATAGCAT TTCTATTAGA ACAAGTAGTC AAACTTTTCA GACTGGAACA 
GTAGAATGGA AATTGCGATC TGATACAGCA CAAGTAGAAC TGACAGGCGA TAACAGTCTT 
CACTCCTATG CTGATTTTTC TGGTGCCACT GAAGTTATTT TGGAAGCAGA ATTAAGCAGA 
GGAGATGGTG ATGTCGCTTG GCAACACACC CAGCTGTTTA GACAAAGCTT AAATGACCAT 
GAAGAAAATT GTTTGGAGAT AATTATAAAA TTCAGTGACC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060767.2
CDS109..2073
Translation

Target ORF information:

RefSeq Version NM_018297.3
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018297.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GGTATATTTG 
GCCCGAAAGG AAGGATCATC TTTTGCTTAT ATTTCCTGGA AGTTTGAGTG TGGGTCAGTT 
GGCCTAAAAG TAGATAGCAT TTCTATTAGA ACAAGTAGTC AAACTTTTCA GACTGGAACA 
GTAGAATGGA AATTGCGATC TGATACAGCA CAAGTAGAAC TGACAGGCGA TAACAGTCTT 
CACTCCTATG CTGATTTTTC TGGTGCCACT GAAGTTATTT TGGAAGCAGA ATTAAGCAGA 
GGAGATGGTG ATGTCGCTTG GCAACACACC CAGCTGTTTA GACAAAGCTT AAATGACCAT 
GAAGAAAATT GTTTGGAGAT AATTATAAAA TTCAGTGACC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18936
Clone ID Related Accession (Same CDS sequence) NM_001145294.1
Accession Version NM_001145294.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1839bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-29
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC329733.1, AK296047.1 and BQ017148.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.56900.1, SRR1803612.283346.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAGAAACC CTAATGATGA AAAATATAGA TCCATCCGGA TTGGAAACAC AGCCTTTTCT 
ACTAGACTCT TGCCTGTCAG AGGAGCTGTT GAATGTTTAT TTGAAATGGG CTTTGAAGAG 
GGAGAAACAC ATCTCATCTT TCCTAAAAAA GCTTCAGTGG AGCAGCTGCA AAAAATTCGT 
GACCTGATTG CCATAGAGAG AAGTAGCAGA CTGGATGGCT CAAATAAGAG CCACAAAGTA 
AAGTCATCTC AGCAACCTGC AGCCAGTACC CAGCTTCCTA CAACACCATC TTCAAATCCC 
AGTGGGTTAA ACCAGCACAC AAGGAACCGT CAAGGGCAGT CATCAGATCC ACCATCTGCT 
TCAACGGTTG CTGCTGACTC AGCCATTCTA GAAGTTCTTC AGTCCAACAT TCAGCATGTG 
CTGGTCTATG AAAATCCTGC TCTTCAGGAG AAAGCGTTGG CTTGTATTCC GGTCCAAGAA 
CTAAAAAGGA AATCACAAGA AAAGTTATCG AGAGCTAGAA AATTGGATAA AGGTATCAAT 
ATAAGTGATG AGGATTTTCT TTTGCTGGAG CTTTTGCACT GGTTTAAGGA AGAATTTTTT 
CACTGGGTGA ATAACGTTTT GTGCAGCAAA TGTGGTGGAC AGACTAGGTC TAGAGATAGA 
TCATTACTGC CCAGTGATGA TGAGCTGAAG TGGGGTGCAA AGGAAGTGGA AGATCATTAC 
TGTGATGCCT GCCAGTTCAG CAATCGATTC CCAAGATATA ATAACCCTGA GAAACTTTTG 
GAAACAAGAT GTGGACGGTG TGGCGAGTGG GCCAATTGTT TTACACTGTG CTGCCGAGCT 
GTAGGGTTTG AAGCTCGCTA TGTTTGGGAT TACACAGACC ATGTCTGGAC AGAAGTCTAT 
TCTCCTTCTC AGCAGCGGTG GCTGCACTGT GATGCATGTG AAGATGTCTG TGACAAGCCA 
CTCCTTTATG AAATAGGATG GGGCAAGAAG CTTTCCTATG TCATAGCATT TTCAAAAGAT 
GAGGTAGTTG ATGTCACTTG GCGATATTCC TGCAAACATG AAGAGGTGAT TGCCAGAAGA 
ACTAAGGTTA AAGAAGCATT ACTTCGAGAC ACTATTAATG GGCTTAATAA GCAGAGGCAA 
CTGTTTTTGT CAGAAAACAG AAGGAAAGAA CTTCTCCAGA GGATAATTGT GGAGCTTGTT 
GAATTTATAT CTCCCAAAAC CCCTAAACCT GGAGAACTTG GGGGAAGAAT ATCTGGGTCA 
GTGGCTTGGA GAGTAGCCCG AGGTGAAATG GGTCTACAGA GAAAAGAAAC CTTGTTTATT 
CCCTGTGAAA ATGAGAAGAT TTCTAAACAG CTCCACCTTT GTTACAATAT TGTGAAAGAT 
CGTTATGTTC GAGTTTCAAA TAACAATCAA ACCATTTCTG GATGGGAGAA TGGCGTGTGG 
AAAATGGAAT CTATATTCAG AAAAGTTGAA ACAGACTGGC ACATGGTATA TTTGGCCCGA 
AAGGAAGGAT CATCTTTTGC TTATATTTCC TGGAAGTTTG AGTGTGGGTC AGTTGGCCTA 
AAAGTAGATA GCATTTCTAT TAGAACAAGT AGTCAAACTT TTCAGACTGG AACAGTAGAA 
TGGAAATTGC GATCTGATAC AGCACAAGTA GAACTGACAG GCGATAACAG TCTTCACTCC 
TATGCTGATT TTTCTGGTGC CACTGAAGTT ATTTTGGAAG CAGAATTAAG CAGAGGAGAT 
GGTGATGTCG CTTGGCAACA CACCCAGCTG TTTAGACAAA GCTTAAATGA CCATGAAGAA 
AATTGTTTGG AGATAATTAT AAAATTCAGT GACCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138766.1
CDS175..2013
Translation

Target ORF information:

RefSeq Version NM_001145294.1
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001145294.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAGAAACC CTAATGATGA AAAATATAGA TCCATCCGGA TTGGAAACAC AGCCTTTTCT 
ACTAGACTCT TGCCTGTCAG AGGAGCTGTT GAATGTTTAT TTGAAATGGG CTTTGAAGAG 
GGAGAAACAC ATCTCATCTT TCCTAAAAAA GCTTCAGTGG AGCAGCTGCA AAAAATTCGT 
GACCTGATTG CCATAGAGAG AAGTAGCAGA CTGGATGGCT CAAATAAGAG CCACAAAGTA 
AAGTCATCTC AGCAACCTGC AGCCAGTACC CAGCTTCCTA CAACACCATC TTCAAATCCC 
AGTGGGTTAA ACCAGCACAC AAGGAACCGT CAAGGGCAGT CATCAGATCC ACCATCTGCT 
TCAACGGTTG CTGCTGACTC AGCCATTCTA GAAGTTCTTC AGTCCAACAT TCAGCATGTG 
CTGGTCTATG AAAATCCTGC TCTTCAGGAG AAAGCGTTGG CTTGTATTCC GGTCCAAGAA 
CTAAAAAGGA AATCACAAGA AAAGTTATCG AGAGCTAGAA AATTGGATAA AGGTATCAAT 
ATAAGTGATG AGGATTTTCT TTTGCTGGAG CTTTTGCACT GGTTTAAGGA AGAATTTTTT 
CACTGGGTGA ATAACGTTTT GTGCAGCAAA TGTGGTGGAC AGACTAGGTC TAGAGATAGA 
TCATTACTGC CCAGTGATGA TGAGCTGAAG TGGGGTGCAA AGGAAGTGGA AGATCATTAC 
TGTGATGCCT GCCAGTTCAG CAATCGATTC CCAAGATATA ATAACCCTGA GAAACTTTTG 
GAAACAAGAT GTGGACGGTG TGGCGAGTGG GCCAATTGTT TTACACTGTG CTGCCGAGCT 
GTAGGGTTTG AAGCTCGCTA TGTTTGGGAT TACACAGACC ATGTCTGGAC AGAAGTCTAT 
TCTCCTTCTC AGCAGCGGTG GCTGCACTGT GATGCATGTG AAGATGTCTG TGACAAGCCA 
CTCCTTTATG AAATAGGATG GGGCAAGAAG CTTTCCTATG TCATAGCATT TTCAAAAGAT 
GAGGTAGTTG ATGTCACTTG GCGATATTCC TGCAAACATG AAGAGGTGAT TGCCAGAAGA 
ACTAAGGTTA AAGAAGCATT ACTTCGAGAC ACTATTAATG GGCTTAATAA GCAGAGGCAA 
CTGTTTTTGT CAGAAAACAG AAGGAAAGAA CTTCTCCAGA GGATAATTGT GGAGCTTGTT 
GAATTTATAT CTCCCAAAAC CCCTAAACCT GGAGAACTTG GGGGAAGAAT ATCTGGGTCA 
GTGGCTTGGA GAGTAGCCCG AGGTGAAATG GGTCTACAGA GAAAAGAAAC CTTGTTTATT 
CCCTGTGAAA ATGAGAAGAT TTCTAAACAG CTCCACCTTT GTTACAATAT TGTGAAAGAT 
CGTTATGTTC GAGTTTCAAA TAACAATCAA ACCATTTCTG GATGGGAGAA TGGCGTGTGG 
AAAATGGAAT CTATATTCAG AAAAGTTGAA ACAGACTGGC ACATGGTATA TTTGGCCCGA 
AAGGAAGGAT CATCTTTTGC TTATATTTCC TGGAAGTTTG AGTGTGGGTC AGTTGGCCTA 
AAAGTAGATA GCATTTCTAT TAGAACAAGT AGTCAAACTT TTCAGACTGG AACAGTAGAA 
TGGAAATTGC GATCTGATAC AGCACAAGTA GAACTGACAG GCGATAACAG TCTTCACTCC 
TATGCTGATT TTTCTGGTGC CACTGAAGTT ATTTTGGAAG CAGAATTAAG CAGAGGAGAT 
GGTGATGTCG CTTGGCAACA CACCCAGCTG TTTAGACAAA GCTTAAATGA CCATGAAGAA 
AATTGTTTGG AGATAATTAT AAAATTCAGT GACCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69736
Clone ID Related Accession (Same CDS sequence) XM_011533944.1
Accession Version XM_011533944.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1734bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGCTTTG AAGAGGGAGA AACACATCTC ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG 
CTGCAAAAAA TTCGTGACCT GATTGCCATA GAGAGAAGTA GCAGACTGGA TGGCTCAAAT 
AAGAGCCACA AAGTAAAGTC ATCTCAGCAA CCTGCAGCCA GTACCCAGCT TCCTACAACA 
CCATCTTCAA ATCCCAGTGG GTTAAACCAG CACACAAGGA ACCGTCAAGG GCAGTCATCA 
GATCCACCAT CTGCTTCAAC GGTTGCTGCT GACTCAGCCA TTCTAGAAGT TCTTCAGTCC 
AACATTCAGC ATGTGCTGGT CTATGAAAAT CCTGCTCTTC AGGAGAAAGC GTTGGCTTGT 
ATTCCGGTCC AAGAACTAAA AAGGAAATCA CAAGAAAAGT TATCGAGAGC TAGAAAATTG 
GATAAAGGTA TCAATATAAG TGATGAGGAT TTTCTTTTGC TGGAGCTTTT GCACTGGTTT 
AAGGAAGAAT TTTTTCACTG GGTGAATAAC GTTTTGTGCA GCAAATGTGG TGGACAGACT 
AGGTCTAGAG ATAGATCATT ACTGCCCAGT GATGATGAGC TGAAGTGGGG TGCAAAGGAA 
GTGGAAGATC ATTACTGTGA TGCCTGCCAG TTCAGCAATC GATTCCCAAG ATATAATAAC 
CCTGAGAAAC TTTTGGAAAC AAGATGTGGA CGGTGTGGCG AGTGGGCCAA TTGTTTTACA 
CTGTGCTGCC GAGCTGTAGG GTTTGAAGCT CGCTATGTTT GGGATTACAC AGACCATGTC 
TGGACAGAAG TCTATTCTCC TTCTCAGCAG CGGTGGCTGC ACTGTGATGC ATGTGAAGAT 
GTCTGTGACA AGCCACTCCT TTATGAAATA GGATGGGGCA AGAAGCTTTC CTATGTCATA 
GCATTTTCAA AAGATGAGGT AGTTGATGTC ACTTGGCGAT ATTCCTGCAA ACATGAAGAG 
GTGATTGCCA GAAGAACTAA GGTTAAAGAA GCATTACTTC GAGACACTAT TAATGGGCTT 
AATAAGCAGA GGCAACTGTT TTTGTCAGAA AACAGAAGGA AAGAACTTCT CCAGAGGATA 
ATTGTGGAGC TTGTTGAATT TATATCTCCC AAAACCCCTA AACCTGGAGA ACTTGGGGGA 
AGAATATCTG GGTCAGTGGC TTGGAGAGTA GCCCGAGGTG AAATGGGTCT ACAGAGAAAA 
GAAACCTTGT TTATTCCCTG TGAAAATGAG AAGATTTCTA AACAGCTCCA CCTTTGTTAC 
AATATTGTGA AAGATCGTTA TGTTCGAGTT TCAAATAACA ATCAAACCAT TTCTGGATGG 
GAGAATGGCG TGTGGAAAAT GGAATCTATA TTCAGAAAAG TTGAAACAGA CTGGCACATG 
GTATATTTGG CCCGAAAGGA AGGATCATCT TTTGCTTATA TTTCCTGGAA GTTTGAGTGT 
GGGTCAGTTG GCCTAAAAGT AGATAGCATT TCTATTAGAA CAAGTAGTCA AACTTTTCAG 
ACTGGAACAG TAGAATGGAA ATTGCGATCT GATACAGCAC AAGTAGAACT GACAGGCGAT 
AACAGTCTTC ACTCCTATGC TGATTTTTCT GGTGCCACTG AAGTTATTTT GGAAGCAGAA 
TTAAGCAGAG GAGATGGTGA TGTCGCTTGG CAACACACCC AGCTGTTTAG ACAAAGCTTA 
AATGACCATG AAGAAAATTG TTTGGAGATA ATTATAAAAT TCAGTGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532246.1
CDS236..1969
Translation

Target ORF information:

RefSeq Version XM_011533944.1
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011533944.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGGCTTTG AAGAGGGAGA AACACATCTC ATCTTTCCTA AAAAAGCTTC AGTGGAGCAG 
CTGCAAAAAA TTCGTGACCT GATTGCCATA GAGAGAAGTA GCAGACTGGA TGGCTCAAAT 
AAGAGCCACA AAGTAAAGTC ATCTCAGCAA CCTGCAGCCA GTACCCAGCT TCCTACAACA 
CCATCTTCAA ATCCCAGTGG GTTAAACCAG CACACAAGGA ACCGTCAAGG GCAGTCATCA 
GATCCACCAT CTGCTTCAAC GGTTGCTGCT GACTCAGCCA TTCTAGAAGT TCTTCAGTCC 
AACATTCAGC ATGTGCTGGT CTATGAAAAT CCTGCTCTTC AGGAGAAAGC GTTGGCTTGT 
ATTCCGGTCC AAGAACTAAA AAGGAAATCA CAAGAAAAGT TATCGAGAGC TAGAAAATTG 
GATAAAGGTA TCAATATAAG TGATGAGGAT TTTCTTTTGC TGGAGCTTTT GCACTGGTTT 
AAGGAAGAAT TTTTTCACTG GGTGAATAAC GTTTTGTGCA GCAAATGTGG TGGACAGACT 
AGGTCTAGAG ATAGATCATT ACTGCCCAGT GATGATGAGC TGAAGTGGGG TGCAAAGGAA 
GTGGAAGATC ATTACTGTGA TGCCTGCCAG TTCAGCAATC GATTCCCAAG ATATAATAAC 
CCTGAGAAAC TTTTGGAAAC AAGATGTGGA CGGTGTGGCG AGTGGGCCAA TTGTTTTACA 
CTGTGCTGCC GAGCTGTAGG GTTTGAAGCT CGCTATGTTT GGGATTACAC AGACCATGTC 
TGGACAGAAG TCTATTCTCC TTCTCAGCAG CGGTGGCTGC ACTGTGATGC ATGTGAAGAT 
GTCTGTGACA AGCCACTCCT TTATGAAATA GGATGGGGCA AGAAGCTTTC CTATGTCATA 
GCATTTTCAA AAGATGAGGT AGTTGATGTC ACTTGGCGAT ATTCCTGCAA ACATGAAGAG 
GTGATTGCCA GAAGAACTAA GGTTAAAGAA GCATTACTTC GAGACACTAT TAATGGGCTT 
AATAAGCAGA GGCAACTGTT TTTGTCAGAA AACAGAAGGA AAGAACTTCT CCAGAGGATA 
ATTGTGGAGC TTGTTGAATT TATATCTCCC AAAACCCCTA AACCTGGAGA ACTTGGGGGA 
AGAATATCTG GGTCAGTGGC TTGGAGAGTA GCCCGAGGTG AAATGGGTCT ACAGAGAAAA 
GAAACCTTGT TTATTCCCTG TGAAAATGAG AAGATTTCTA AACAGCTCCA CCTTTGTTAC 
AATATTGTGA AAGATCGTTA TGTTCGAGTT TCAAATAACA ATCAAACCAT TTCTGGATGG 
GAGAATGGCG TGTGGAAAAT GGAATCTATA TTCAGAAAAG TTGAAACAGA CTGGCACATG 
GTATATTTGG CCCGAAAGGA AGGATCATCT TTTGCTTATA TTTCCTGGAA GTTTGAGTGT 
GGGTCAGTTG GCCTAAAAGT AGATAGCATT TCTATTAGAA CAAGTAGTCA AACTTTTCAG 
ACTGGAACAG TAGAATGGAA ATTGCGATCT GATACAGCAC AAGTAGAACT GACAGGCGAT 
AACAGTCTTC ACTCCTATGC TGATTTTTCT GGTGCCACTG AAGTTATTTT GGAAGCAGAA 
TTAAGCAGAG GAGATGGTGA TGTCGCTTGG CAACACACCC AGCTGTTTAG ACAAAGCTTA 
AATGACCATG AAGAAAATTG TTTGGAGATA ATTATAAAAT TCAGTGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11077
Clone ID Related Accession (Same CDS sequence) NM_001145293.1
Accession Version NM_001145293.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-29
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092798.3, BC007226.1, BC000963.2 and BQ017148.1. Transcript Variant: This variant (2) includes an alternate exon that causes a frameshift and lacks an alternate exon which restores the reading frame, compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.111671.1, SRR1803612.34076.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGAGTTACA GCGCACATTA 
AGCCTAAAAC TGACCACGTT AAAGGAGATT GGAAAAACAT TTTTAAGGAT TTCAAAGAGA 
CAGAAATTAA TACAGGTAGT TGATGTCACT TGGCGATATT CCTGCAAACA TGAAGAGGTG 
ATTGCCAGAA GAACTAAGGT TAAAGAAGCA TTACTTCGAG ACACTATTAA TGGGCTTAAT 
AAGCAGAGGC AACTGTTTTT GTCAGAAAAC AGAAGGAAAG AACTTCTCCA GAGGATAATT 
GTGGAGCTTG TTGAATTTAT ATCTCCCAAA ACCCCTAAAC CTGGAGAACT TGGGGGAAGA 
ATATCTGGGT CAGTGGCTTG GAGAGTAGCC CGAGGTGAAA TGGGTCTACA GAGAAAAGAA 
ACCTTGTTTA TTCCCTGTGA AAATGAGAAG ATTTCTAAAC AGCTCCACCT TTGTTACAAT 
ATTGTGAAAG ATCGTTATGT TCGAGTTTCA AATAACAATC AAACCATTTC TGGATGGGAG 
AATGGCGTGT GGAAAATGGA ATCTATATTC AGAAAAGTTG AAACAGACTG GCACATGGTA 
TATTTGGCCC GAAAGGAAGG ATCATCTTTT GCTTATATTT CCTGGAAGTT TGAGTGTGGG 
TCAGTTGGCC TAAAAGTAGA TAGCATTTCT ATTAGAACAA GTAGTCAAAC TTTTCAGACT 
GGAACAGTAG AATGGAAATT GCGATCTGAT ACAGCACAAG TAGAACTGAC AGGCGATAAC 
AGTCTTCACT CCTATGCTGA TTTTTCTGGT GCCACTGAAG TTATTTTGGA AGCAGAATTA 
AGCAGAGGAG ATGGTGATGT CGCTTGGCAA CACACCCAGC TGTTTAGACA AAGCTTAAAT 
GACCATGAAG AAAATTGTTT GGAGATAATT ATAAAATTCA GTGACCTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138765.1
CDS109..2019
Translation

Target ORF information:

RefSeq Version NM_001145293.1
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001145293.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGAGTTACA GCGCACATTA 
AGCCTAAAAC TGACCACGTT AAAGGAGATT GGAAAAACAT TTTTAAGGAT TTCAAAGAGA 
CAGAAATTAA TACAGGTAGT TGATGTCACT TGGCGATATT CCTGCAAACA TGAAGAGGTG 
ATTGCCAGAA GAACTAAGGT TAAAGAAGCA TTACTTCGAG ACACTATTAA TGGGCTTAAT 
AAGCAGAGGC AACTGTTTTT GTCAGAAAAC AGAAGGAAAG AACTTCTCCA GAGGATAATT 
GTGGAGCTTG TTGAATTTAT ATCTCCCAAA ACCCCTAAAC CTGGAGAACT TGGGGGAAGA 
ATATCTGGGT CAGTGGCTTG GAGAGTAGCC CGAGGTGAAA TGGGTCTACA GAGAAAAGAA 
ACCTTGTTTA TTCCCTGTGA AAATGAGAAG ATTTCTAAAC AGCTCCACCT TTGTTACAAT 
ATTGTGAAAG ATCGTTATGT TCGAGTTTCA AATAACAATC AAACCATTTC TGGATGGGAG 
AATGGCGTGT GGAAAATGGA ATCTATATTC AGAAAAGTTG AAACAGACTG GCACATGGTA 
TATTTGGCCC GAAAGGAAGG ATCATCTTTT GCTTATATTT CCTGGAAGTT TGAGTGTGGG 
TCAGTTGGCC TAAAAGTAGA TAGCATTTCT ATTAGAACAA GTAGTCAAAC TTTTCAGACT 
GGAACAGTAG AATGGAAATT GCGATCTGAT ACAGCACAAG TAGAACTGAC AGGCGATAAC 
AGTCTTCACT CCTATGCTGA TTTTTCTGGT GCCACTGAAG TTATTTTGGA AGCAGAATTA 
AGCAGAGGAG ATGGTGATGT CGCTTGGCAA CACACCCAGC TGTTTAGACA AAGCTTAAAT 
GACCATGAAG AAAATTGTTT GGAGATAATT ATAAAATTCA GTGACCTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu12482
Clone ID Related Accession (Same CDS sequence) NM_001145295.1
Accession Version NM_001145295.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-29
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform 4
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092798.3, BC007226.1, BC017220.2 and BQ017148.1. Transcript Variant: This variant (4) lacks an exon in the 3' coding region, which results in a frameshift and an early stop codon compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.29099.1, SRR1803612.96704.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GATAACAGTC 
TTCACTCCTA TGCTGATTTT TCTGGTGCCA CTGAAGTTAT TTTGGAAGCA GAATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138767.1
CDS109..1785
Translation

Target ORF information:

RefSeq Version NM_001145295.1
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001145295.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GATAACAGTC 
TTCACTCCTA TGCTGATTTT TCTGGTGCCA CTGAAGTTAT TTTGGAAGCA GAATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37371
Clone ID Related Accession (Same CDS sequence) XM_005265316.1
Accession Version XM_005265316.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGAAAAGAAA CCTTGTTTAT TCCCTGTGAA AATGAGAAGA TTTCTAAACA GCTCCACCTT 
TGTTACAATA TTGTGAAAGA TCGTTATGTT CGAGTTTCAA ATAACAATCA AACCATTTCT 
GGATGGGAGA ATGGCGTGTG GAAAATGGAA TCTATATTCA GAAAAGTTGA AACAGACTGG 
CACATGGTAT ATTTGGCCCG AAAGGAAGGA TCATCTTTTG CTTATATTTC CTGGAAGTTT 
GAGTGTGGGT CAGTTGGCCT AAAAGTAGAT AGCATTTCTA TTAGAACAAG TAGTCAAACT 
TTTCAGACTG GAACAGTAGA ATGGAAATTG CGATCTGATA CAGCACAAGT AGAACTGACA 
GGCGATAACA GTCTTCACTC CTATGCTGAT TTTTCTGGTG CCACTGAAGT TATTTTGGAA 
GCAGAATTAA GCAGAGGAGA TGGTGATGTC GCTTGGCAAC ACACCCAGCT GTTTAGACAA 
AGCTTAAATG ACCATGAAGA AAATTGTTTG GAGATAATTA TAAAATTCAG TGACCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265373.1
CDS54..1853
Translation

Target ORF information:

RefSeq Version XM_005265316.1
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005265316.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGAAAAGAAA CCTTGTTTAT TCCCTGTGAA AATGAGAAGA TTTCTAAACA GCTCCACCTT 
TGTTACAATA TTGTGAAAGA TCGTTATGTT CGAGTTTCAA ATAACAATCA AACCATTTCT 
GGATGGGAGA ATGGCGTGTG GAAAATGGAA TCTATATTCA GAAAAGTTGA AACAGACTGG 
CACATGGTAT ATTTGGCCCG AAAGGAAGGA TCATCTTTTG CTTATATTTC CTGGAAGTTT 
GAGTGTGGGT CAGTTGGCCT AAAAGTAGAT AGCATTTCTA TTAGAACAAG TAGTCAAACT 
TTTCAGACTG GAACAGTAGA ATGGAAATTG CGATCTGATA CAGCACAAGT AGAACTGACA 
GGCGATAACA GTCTTCACTC CTATGCTGAT TTTTCTGGTG CCACTGAAGT TATTTTGGAA 
GCAGAATTAA GCAGAGGAGA TGGTGATGTC GCTTGGCAAC ACACCCAGCT GTTTAGACAA 
AGCTTAAATG ACCATGAAGA AAATTGTTTG GAGATAATTA TAAAATTCAG TGACCTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37372
Clone ID Related Accession (Same CDS sequence) XM_005265317.1
Accession Version XM_005265317.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGAAAAGAAA CCTTGTTTAT TCCCTGTGAA AATGAGAAGA TTTCTAAACA GCTCCACCTT 
TGTTACAATA TTGTGAAAGA TCGTTATGTT CGAGTTTCAA ATAACAATCA AACCATTTCT 
GGATGGGAGA ATGGCGTGTG GAAAATGGAA TCTATATTCA GAAAAGTTGA AACAGACTGG 
CACATGATAA CAGTCTTCAC TCCTATGCTG ATTTTTCTGG TGCCACTGAA GTTATTTTGG 
AAGCAGAATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265374.1
CDS54..1565
Translation

Target ORF information:

RefSeq Version XM_005265317.1
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005265317.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGAAAAGAAA CCTTGTTTAT TCCCTGTGAA AATGAGAAGA TTTCTAAACA GCTCCACCTT 
TGTTACAATA TTGTGAAAGA TCGTTATGTT CGAGTTTCAA ATAACAATCA AACCATTTCT 
GGATGGGAGA ATGGCGTGTG GAAAATGGAA TCTATATTCA GAAAAGTTGA AACAGACTGG 
CACATGATAA CAGTCTTCAC TCCTATGCTG ATTTTTCTGG TGCCACTGAA GTTATTTTGG 
AAGCAGAATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69737
Clone ID Related Accession (Same CDS sequence) XM_011533945.2
Accession Version XM_011533945.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGGTAGTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532247.1
CDS54..1064
Translation

Target ORF information:

RefSeq Version XM_011533945.2
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011533945.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGGTAGTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu94383
Clone ID Related Accession (Same CDS sequence) XM_017006839.2 , XM_017006839.1
Accession Version XM_017006839.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1776bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GATATCCCCC 
TTTGCTAGCC ATTCCTTTAA AGGAGCAGTT AACCTGTGTG TAACCCAGAT GCCTAATAGT 
TTAGGATGGG TGAAACTAAT GACTCCCGGG GAACACTGGT GGAGTCCTGA GTCCCACATC 
CTCCTTACAA GCGGCTGTAA GAGGAGCATG CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016862328.1
CDS54..1829
Translation

Target ORF information:

RefSeq Version XM_017006839.2
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017006839.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GATATCCCCC 
TTTGCTAGCC ATTCCTTTAA AGGAGCAGTT AACCTGTGTG TAACCCAGAT GCCTAATAGT 
TTAGGATGGG TGAAACTAAT GACTCCCGGG GAACACTGGT GGAGTCCTGA GTCCCACATC 
CTCCTTACAA GCGGCTGTAA GAGGAGCATG CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu94383
Clone ID Related Accession (Same CDS sequence) XM_017006839.2 , XM_017006839.1
Accession Version XM_017006839.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1776bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GATATCCCCC 
TTTGCTAGCC ATTCCTTTAA AGGAGCAGTT AACCTGTGTG TAACCCAGAT GCCTAATAGT 
TTAGGATGGG TGAAACTAAT GACTCCCGGG GAACACTGGT GGAGTCCTGA GTCCCACATC 
CTCCTTACAA GCGGCTGTAA GAGGAGCATG CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016862328.1
CDS54..1829
Translation

Target ORF information:

RefSeq Version XM_017006839.1
Organism Homo sapiens(human)
Definition Homo sapiens N-glycanase 1 (NGLY1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017006839.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CGGCATTGGG CAGCTCCTCA GGCTCGGCGT CCCCGGCCGT GGCTGAGCTC 
TGCCAGAACA CCCCGGAGAC CTTTTTGGAG GCCTCCAAGC TGCTGCTCAC CTATGCTGAC 
AACATCCTCA GAAACCCTAA TGATGAAAAA TATAGATCCA TCCGGATTGG AAACACAGCC 
TTTTCTACTA GACTCTTGCC TGTCAGAGGA GCTGTTGAAT GTTTATTTGA AATGGGCTTT 
GAAGAGGGAG AAACACATCT CATCTTTCCT AAAAAAGCTT CAGTGGAGCA GCTGCAAAAA 
ATTCGTGACC TGATTGCCAT AGAGAGAAGT AGCAGACTGG ATGGCTCAAA TAAGAGCCAC 
AAAGTAAAGT CATCTCAGCA ACCTGCAGCC AGTACCCAGC TTCCTACAAC ACCATCTTCA 
AATCCCAGTG GGTTAAACCA GCACACAAGG AACCGTCAAG GGCAGTCATC AGATCCACCA 
TCTGCTTCAA CGGTTGCTGC TGACTCAGCC ATTCTAGAAG TTCTTCAGTC CAACATTCAG 
CATGTGCTGG TCTATGAAAA TCCTGCTCTT CAGGAGAAAG CGTTGGCTTG TATTCCGGTC 
CAAGAACTAA AAAGGAAATC ACAAGAAAAG TTATCGAGAG CTAGAAAATT GGATAAAGGT 
ATCAATATAA GTGATGAGGA TTTTCTTTTG CTGGAGCTTT TGCACTGGTT TAAGGAAGAA 
TTTTTTCACT GGGTGAATAA CGTTTTGTGC AGCAAATGTG GTGGACAGAC TAGGTCTAGA 
GATAGATCAT TACTGCCCAG TGATGATGAG CTGAAGTGGG GTGCAAAGGA AGTGGAAGAT 
CATTACTGTG ATGCCTGCCA GTTCAGCAAT CGATTCCCAA GATATAATAA CCCTGAGAAA 
CTTTTGGAAA CAAGATGTGG ACGGTGTGGC GAGTGGGCCA ATTGTTTTAC ACTGTGCTGC 
CGAGCTGTAG GGTTTGAAGC TCGCTATGTT TGGGATTACA CAGACCATGT CTGGACAGAA 
GTCTATTCTC CTTCTCAGCA GCGGTGGCTG CACTGTGATG CATGTGAAGA TGTCTGTGAC 
AAGCCACTCC TTTATGAAAT AGGATGGGGC AAGAAGCTTT CCTATGTCAT AGCATTTTCA 
AAAGATGAGG TAGTTGATGT CACTTGGCGA TATTCCTGCA AACATGAAGA GGTGATTGCC 
AGAAGAACTA AGGTTAAAGA AGCATTACTT CGAGACACTA TTAATGGGCT TAATAAGCAG 
AGGCAACTGT TTTTGTCAGA AAACAGAAGG AAAGAACTTC TCCAGAGGAT AATTGTGGAG 
CTTGTTGAAT TTATATCTCC CAAAACCCCT AAACCTGGAG AACTTGGGGG AAGAATATCT 
GGGTCAGTGG CTTGGAGAGT AGCCCGAGGT GAAATGGGTC TACAGAGAAA AGAAACCTTG 
TTTATTCCCT GTGAAAATGA GAAGATTTCT AAACAGCTCC ACCTTTGTTA CAATATTGTG 
AAAGATCGTT ATGTTCGAGT TTCAAATAAC AATCAAACCA TTTCTGGATG GGAGAATGGC 
GTGTGGAAAA TGGAATCTAT ATTCAGAAAA GTTGAAACAG ACTGGCACAT GATATCCCCC 
TTTGCTAGCC ATTCCTTTAA AGGAGCAGTT AACCTGTGTG TAACCCAGAT GCCTAATAGT 
TTAGGATGGG TGAAACTAAT GACTCCCGGG GAACACTGGT GGAGTCCTGA GTCCCACATC 
CTCCTTACAA GCGGCTGTAA GAGGAGCATG CAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Mitochondrial function requires NGLY1.
Mitochondrion386-16(2018 Jan)
Kong J,Peng M,Ostrovsky J,Kwon YJ,Oretsky O,McCormick EM,He M,Argon Y,Falk MJ


book

Tissue-specific regulation of BMP signaling by
eLife6(2017 Aug)
Galeone A,Han SY,Huang C,Hosomi A,Suzuki T,Jafar-Nejad H


book

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW


book

Prospective phenotyping of NGLY1-CDDG, the first congenital disorder of deglycosylation.
Genetics in medicine : official journal of the American College of Medical Genetics19(2)160-168(2017 Feb)
Lam C,Ferreira C,Krasnewich D,Toro C,Latham L,Zein WM,Lehky T,Brewer C,Baker EH,Thurm A,Farmer CA,Rosenzweig SD,Lyons JJ,Schreiber JM,Gropman A,Lingala S,Ghany MG,Solomon B,Macnamara E,Davids M,Stratakis CA,Kimonis V,Gahl WA,Wolfe L