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PRTN3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PRTN3
Entrez Gene ID 5657
Full Name proteinase 3
Synonyms ACPA,AGP7,C-ANCA,CANCA,MBN,MBT,NP-4,NP4,P29,PR-3,PR3
General protein information
Preferred Names

proteinase 3

Names

myeloblastin
C-ANCA antigen
Wegener granulomatosis autoantigen
azurophil granule protein 7
leukocyte proteinase 3
neutrophil proteinase 4
serine proteinase, neutrophil
wegener autoantigen

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

19

19p13.3

Disorder MIM:

177020


mRNA and Protein(s)

mRNA Protein Name
NM_002777.3 NP_002768.3 myeloblastin precursor
XM_011528136.1 XP_011526438.1 myeloblastin isoform X1



Homo sapiens (human) PRTN3 NP_002768.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007141 XP_565054.3
Macaca mulatta (rhesus monkey) LOC721131 XP_001117176.2
Anopheles gambiae (African malaria mosquito) CTR2_ANOGA XP_309032.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP006710 XP_565496.1
Xenopus tropicalis (tropical clawed frog) prtn3 NP_001006847.1
Rattus norvegicus (Norway rat) Prtn3 NP_001019435.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007142 XP_308619.3
Mus musculus (house mouse) Prtn3 NP_035308.2
Anopheles gambiae (African malaria mosquito) CTR1_ANOGA XP_309033.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP006707 XP_309036.1
Bos taurus (cattle) PRTN3 XP_002689189.1


Related articles in PubMed

NETosis-associated serum biomarkers are reduced in type 1 diabetes in association with neutrophil count.
Qin J, Fu S, Speake C, Greenbaum CJ, Odegard JM
Clinical and experimental immunology184(3)318-22(2016 Jun)

Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.
Martin KR, Kantari-Mimoun C, Yin M, Pederzoli-Ribeil M, Angelot-Delettre F, Ceroi A, Grauffel C, Benhamou M, Reuter N, Saas P, Frachet P, Boulanger CM, Witko-Sarsat V
The Journal of biological chemistry291(20)10476-89(2016 May)

Clinical Implication of Proteinase-3-antineutrophil Cytoplasmic Antibody in Patients with Idiopathic Interstitial Pneumonias.
Hozumi H, Enomoto N, Oyama Y, Kono M, Fujisawa T, Inui N, Nakamura Y, Suda T
Lung194(2)235-42(2016 Apr)

Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis.
Yang J, Ge H, Poulton CJ, Hogan SL, Hu Y, Jones BE, Henderson CD, McInnis EA, Pendergraft WF 3rd, Jennette JC, Falk RJ, Ciavatta DJ
Clinical epigenetics885(2016)

Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis.
Millet A, Martin KR, Bonnefoy F, Saas P, Mocek J, Alkan M, Terrier B, Kerstein A, Tamassia N, Satyanarayanan SK, Ariel A, Ribeil JA, Guillevin L, Cassatella MA, Mueller A, Thieblemont N, Lamprecht P, Mouthon L, Perruche S, Witko-Sarsat V
The Journal of clinical investigation125(11)4107-21(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PRTN3 variants are associated with ANCA-associated vasculitis risk.
Title: Identification of Functional and Expression Polymorphisms Associated With Risk for Antineutrophil Cytoplasmic Autoantibody-Associated Vasculitis.

Changes in the DNA methylation status of the PRTN3 promoter may predict the likelihood of stable remission and explain autoantigen gene regulation
Title: Gene-Specific DNA Methylation Changes Predict Remission in Patients with ANCA-Associated Vasculitis.

Clinical manifestations varied ANCA-associated vasculitis categories, and neither MPO-ANCA nor PR3-ANCA significantly affected relapse of AAV.
Title: Clinical and prognostic features of Korean patients with MPO-ANCA, PR3-ANCA and ANCA-negative vasculitis.

Molecular simulations suggest that PR3 interacts with phosphatidylserine via a small number of amino acids, which engage in long lasting interactions with the lipid heads. As phosphatidylserine is a major component of microvesicles (MVs), this study also examined the consequences of this interaction on MV production and function.
Title: Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.

Study shows that MPO and PRTN3 in neutrophils of Anti-neutrophil cytoplasmic autoantibody (ANCA)-associated vasculitis (AAV) patients with active disease have a distinct pattern of histone modifications, which implicates epigenetic mechanisms in regulating expression of autoantigen genes and suggests that the epigenome may be involved in AAV pathogenesis.
Title: Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis.

The following PRTN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRTN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25395 NM_002777.3
Latest version!
Homo sapiens proteinase 3 (PRTN3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu59976 XM_011528136.1
Latest version!
Homo sapiens proteinase 3 (PRTN3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

ORF Online Only Promotion

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1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25395
Clone ID Related Accession (Same CDS sequence) NM_002777.3
Accession Version NM_002777.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 771bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product myeloblastin precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M75154.1, BC096185.3 and AC004799.2. On Jul 28, 2005 this sequence version replaced NM_002777.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X55668.1, M75154.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145893, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCACC GGCCCCCCAG CCCTGCCCTG GCGTCCGTGC TGCTGGCCTT GCTGCTGAGC 
GGTGCTGCCC GAGCTGCGGA GATCGTGGGC GGGCACGAGG CGCAGCCACA CTCCCGGCCC
TACATGGCCT CCCTGCAGAT GCGGGGGAAC CCGGGCAGCC ACTTCTGCGG AGGCACCTTG
ATCCACCCCA GCTTCGTGCT GACGGCCGCG CACTGCCTGC GGGACATACC CCAGCGCCTG
GTGAACGTGG TGCTCGGAGC CCACAACGTG CGGACGCAGG AGCCCACCCA GCAGCACTTC
TCGGTGGCTC AGGTGTTTCT GAACAACTAC GACGCGGAGA ACAAACTGAA CGACGTTCTC
CTCATCCAGC TGAGCAGCCC AGCCAACCTC AGTGCCTCCG TCGCCACAGT CCAGCTGCCA
CAGCAGGACC AGCCAGTGCC CCACGGCACC CAGTGCCTGG CCATGGGCTG GGGCCGCGTG
GGTGCCCACG ACCCCCCAGC CCAGGTCCTG CAGGAGCTCA ATGTCACCGT GGTCACCTTC
TTCTGCCGGC CACATAACAT TTGCACTTTC GTCCCTCGCC GCAAGGCCGG CATCTGCTTC
GGAGACTCAG GTGGCCCCCT GATCTGTGAT GGCATCATCC AAGGAATAGA CTCCTTCGTG
ATCTGGGGAT GTGCCACCCG CCTTTTCCCT GACTTCTTCA CGCGGGTAGC CCTCTACGTG
GACTGGATCC GTTCCACGCT GCGCCGTGTG GAGGCCAAGG GCCGCCCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002768.3
CDS25..795
Misc Feature(1)103..753
Misc Feature(2)106..762
Misc Feature(3)106..108
Misc Feature(4)order(235..237,376..378,631..633)
Misc Feature(5)order(613..615,676..678,682..684)
Exon (1)1..85
Exon (2)86..251
Exon (3)252..393
Exon (4)394..624
Exon (5)625..1001
Translation

Target ORF information:

RefSeq Version NM_002777.3
Organism Homo sapiens(Human)
Definition Homo sapiens proteinase 3 (PRTN3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002777.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCTCACC GGCCCCCCAG CCCTGCCCTG GCGTCCGTGC TGCTGGCCTT GCTGCTGAGC 
GGTGCTGCCC GAGCTGCGGA GATCGTGGGC GGGCACGAGG CGCAGCCACA CTCCCGGCCC
TACATGGCCT CCCTGCAGAT GCGGGGGAAC CCGGGCAGCC ACTTCTGCGG AGGCACCTTG
ATCCACCCCA GCTTCGTGCT GACGGCCGCG CACTGCCTGC GGGACATACC CCAGCGCCTG
GTGAACGTGG TGCTCGGAGC CCACAACGTG CGGACGCAGG AGCCCACCCA GCAGCACTTC
TCGGTGGCTC AGGTGTTTCT GAACAACTAC GACGCGGAGA ACAAACTGAA CGACGTTCTC
CTCATCCAGC TGAGCAGCCC AGCCAACCTC AGTGCCTCCG TCGCCACAGT CCAGCTGCCA
CAGCAGGACC AGCCAGTGCC CCACGGCACC CAGTGCCTGG CCATGGGCTG GGGCCGCGTG
GGTGCCCACG ACCCCCCAGC CCAGGTCCTG CAGGAGCTCA ATGTCACCGT GGTCACCTTC
TTCTGCCGGC CACATAACAT TTGCACTTTC GTCCCTCGCC GCAAGGCCGG CATCTGCTTC
GGAGACTCAG GTGGCCCCCT GATCTGTGAT GGCATCATCC AAGGAATAGA CTCCTTCGTG
ATCTGGGGAT GTGCCACCCG CCTTTTCCCT GACTTCTTCA CGCGGGTAGC CCTCTACGTG
GACTGGATCC GTTCCACGCT GCGCCGTGTG GAGGCCAAGG GCCGCCCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59976
Clone ID Related Accession (Same CDS sequence) XM_011528136.1
Accession Version XM_011528136.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product myeloblastin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCACC GGCCCCCCAG CCCTGCCCTG GCGTCCGTGC TGCTGGCCTT GCTGCTGAGC 
GGTGCTGCCC GAGCTGCGGA GATCGTGGGC GGGCACGAGG CGCAGCCACA CTCCCGGCCC
TACATGGCCT CCCTGCAGAT GCGGGGGAAC CCGGGCAGCC ACTTCTGCGG AGGCACCTTG
ATCCACCCCA GCTTCGTGCT GACGGCCGCG CACTGCCTGC GGGACATACC CCAGCGCCTG
GTGAACGTGG TGCTCGGAGC CCACAACGTG CGGACGCAGG AGCCCACCCA GCAGCACTTC
TCGGTGGCTC AGGTGTTTCT GAACAACTAC GACGCGGAGA ACAAACTGAA CGACGTTCTC
CTCATCCAGC TGAGCAGCCC AGCCAACCTC AGTGCCTCCG TCGCCACAGT CCAGCTGCCA
CAGCAGGACC AGCCAGTGCC CCACGGCACC CAGTGCCTGG CCATGGGCTG GGGCCGCGTG
GGTGCCCACG ACCCCCCAGC CCAGGTCCTG CAGGAGCTCA ATGTCACCGT GGTCACCTTC
TTCTGCCGGC CACATAACAT TTGCACTTTC GTCCCTCGCC GCAAGGCCGG CATCTGCTTC
GGAGACTCAG GTGGCCCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011526438.1
CDS45..665
Misc Feature(1)123..662
Misc Feature(2)126..662
Misc Feature(3)126..128
Misc Feature(4)order(255..257,396..398,651..653)
Translation

Target ORF information:

RefSeq Version XM_011528136.1
Organism Homo sapiens(Human)
Definition Homo sapiens proteinase 3 (PRTN3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011528136.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTCACC GGCCCCCCAG CCCTGCCCTG GCGTCCGTGC TGCTGGCCTT GCTGCTGAGC 
GGTGCTGCCC GAGCTGCGGA GATCGTGGGC GGGCACGAGG CGCAGCCACA CTCCCGGCCC
TACATGGCCT CCCTGCAGAT GCGGGGGAAC CCGGGCAGCC ACTTCTGCGG AGGCACCTTG
ATCCACCCCA GCTTCGTGCT GACGGCCGCG CACTGCCTGC GGGACATACC CCAGCGCCTG
GTGAACGTGG TGCTCGGAGC CCACAACGTG CGGACGCAGG AGCCCACCCA GCAGCACTTC
TCGGTGGCTC AGGTGTTTCT GAACAACTAC GACGCGGAGA ACAAACTGAA CGACGTTCTC
CTCATCCAGC TGAGCAGCCC AGCCAACCTC AGTGCCTCCG TCGCCACAGT CCAGCTGCCA
CAGCAGGACC AGCCAGTGCC CCACGGCACC CAGTGCCTGG CCATGGGCTG GGGCCGCGTG
GGTGCCCACG ACCCCCCAGC CCAGGTCCTG CAGGAGCTCA ATGTCACCGT GGTCACCTTC
TTCTGCCGGC CACATAACAT TTGCACTTTC GTCCCTCGCC GCAAGGCCGG CATCTGCTTC
GGAGACTCAG GTGGCCCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

NETosis-associated serum biomarkers are reduced in type 1 diabetes in association with neutrophil count.
Clinical and experimental immunology184(3)318-22(2016 Jun)
Qin J,Fu S,Speake C,Greenbaum CJ,Odegard JM


book

Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.
The Journal of biological chemistry291(20)10476-89(2016 May)
Martin KR,Kantari-Mimoun C,Yin M,Pederzoli-Ribeil M,Angelot-Delettre F,Ceroi A,Grauffel C,Benhamou M,Reuter N,Saas P,Frachet P,Boulanger CM,Witko-Sarsat V


book

Clinical Implication of Proteinase-3-antineutrophil Cytoplasmic Antibody in Patients with Idiopathic Interstitial Pneumonias.
Lung194(2)235-42(2016 Apr)
Hozumi H,Enomoto N,Oyama Y,Kono M,Fujisawa T,Inui N,Nakamura Y,Suda T


book

Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis.
Clinical epigenetics885(2016)
Yang J,Ge H,Poulton CJ,Hogan SL,Hu Y,Jones BE,Henderson CD,McInnis EA,Pendergraft WF 3rd,Jennette JC,Falk RJ,Ciavatta DJ


book

Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis.
The Journal of clinical investigation125(11)4107-21(2015 Nov)
Millet A,Martin KR,Bonnefoy F,Saas P,Mocek J,Alkan M,Terrier B,Kerstein A,Tamassia N,Satyanarayanan SK,Ariel A,Ribeil JA,Guillevin L,Cassatella MA,Mueller A,Thieblemont N,Lamprecht P,Mouthon L,Perruche S,Witko-Sarsat V


 
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