SGCG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SGCG
Entrez Gene ID	6445
Full Name	sarcoglycan gamma
Synonyms	35DAG,A4,DAGA4,DMDA,DMDA1,LGMD2C,MAM,SCARMD2,SCG3,gamma-SG
General protein information	Preferred Names sarcoglycan gamma Names gamma-sarcoglycan 35 kDa dystrophin-associated glycoprotein 35kD dystrophin-associated glycoprotein sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.12
Summary	This gene encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq, Oct 2008].
Disorder MIM:	608896
Disorder Html:	Muscular dystrophy, limb-girdle, type 2C, 253700 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000231.2	NP_000222.1	gamma-sarcoglycan
XM_005266505.2	XP_005266562.1	gamma-sarcoglycan isoform X2
XM_024449397.1	XP_024305165.1	gamma-sarcoglycan isoform X2
XM_006719861.3	XP_006719924.1	gamma-sarcoglycan isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sgcg	NP_001006994.1
Mus musculus (house mouse)	Sgcg	NP_036022.1
Xenopus tropicalis (tropical clawed frog)	sgcg	NP_001072908.1
Homo sapiens (human)	SGCG	NP_000222.1
Canis lupus familiaris (dog)	SGCG	NP_001014298.1
Pan troglodytes (chimpanzee)	SGCG	XP_001151216.1
Bos taurus (cattle)	SGCG	NP_001068712.1
Gallus gallus (chicken)	SGCG	XP_417139.2
Danio rerio (zebrafish)	LOC100538014	XP_003200060.1
	LOC721411	XP_001117551.2
Danio rerio (zebrafish)	sgcg	NP_001003748.1

Gene Ontology
Bibliography

Related articles in PubMed

?-sarcoglycan and dystrophin mutation spectrum in an Algerian cohort.
Dalichaouche I, Sifi Y, Roudaut C, Sifi K, Hamri A, Rouabah L, Abadi N, Richard I
Muscle & nerve56(1)129-135(2017 Jul)

Crystal Structures of SgcE6 and SgcC, the Two-Component Monooxygenase That Catalyzes Hydroxylation of a Carrier Protein-Tethered Substrate during the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027 in Streptomyces globisporus.
Chang CY, Lohman JR, Cao H, Tan K, Rudolf JD, Ma M, Xu W, Bingman CA, Yennamalli RM, Bigelow L, Babnigg G, Yan X, Joachimiak A, Phillips GN Jr, Shen B
Biochemistry55(36)5142-54(2016 09)

Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle.
Spinazzola JM, Smith TC, Liu M, Luna EJ, Barton ER
Human molecular genetics24(9)2470-81(2015 May)

Carrier frequency of the c.525delT mutation in the SGCG gene and estimated prevalence of limb girdle muscular dystrophy type 2C among the Moroccan population.
El Kerch F, Ratbi I, Sbiti A, Laarabi FZ, Barkat A, Sefiani A
Genetic testing and molecular biomarkers18(4)253-6(2014 Apr)

Puerto Rican founder mutation G787A in the SGCG gene: a case report of 2 siblings with LGMD 2C.
DiCapua D, Patwa H
Journal of clinical neuromuscular disease15(3)105-7(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that fifteen families were shown to carry SGCG variants in patient with early onset severe muscular dystrophy.
Title: ?-sarcoglycan and dystrophin mutation spectrum in an Algerian cohort.

FADH2-dependent monooxygenase (SgcE6 and SgcC) that catalyzes the hydroxylation of a PCP-tethered substrate
Title: Crystal Structures of SgcE6 and SgcC, the Two-Component Monooxygenase That Catalyzes Hydroxylation of a Carrier Protein-Tethered Substrate during the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027 in Streptomyces globisporus.

These results position archvillin as a mechanically sensitive component of the dystrophin complex and demonstrate that signaling defects caused by loss of gamma-SG occur both at the sarcolemma and in the nucleus.
Title: Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle.

Molecular epidemiologic methods were used to calculate the frequency of heterozygotes for this SGCG mutation in Moroccan newborns and to estimate the prevalence of LGMD2C in the Moroccan population.
Title: Carrier frequency of the c.525delT mutation in the SGCG gene and estimated prevalence of limb girdle muscular dystrophy type 2C among the Moroccan population.

A report of two siblings with severe childhood onset limb-girdle muscular dystrophy type 2C supports the theory that the mutation G787A in the SGCG gene is a founder mutation.
Title: Puerto Rican founder mutation G787A in the SGCG gene: a case report of 2 siblings with LGMD 2C.

The following SGCG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGCG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26253	NM_000231.2 Latest version!	Homo sapiens sarcoglycan gamma (SGCG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu47253	XM_006719861.3 Latest version!	Homo sapiens sarcoglycan gamma (SGCG), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu47254	XM_005266505.2 Latest version!	Homo sapiens sarcoglycan gamma (SGCG), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu47254	XM_024449397.1 Latest version!	Homo sapiens sarcoglycan gamma (SGCG), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SGCG ( NM_000231.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000222.1 Homo sapiens sarcoglycan gamma (SGCG), mRNA.

Price & Availability↑

CloneID	OHu26253
Clone ID Related Accession (Same CDS sequence)	NM_000231.2
Accession Version	NM_000231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	gamma-sarcoglycan
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356287.16, DA897096.1, BC074778.2 and U34976.1. This sequence is a reference standard in the RefSeqGene project. On Oct 16, 2008 this sequence version replaced NM_000231.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U34976.1, ERR279837.528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCGTG AGCAGTACAC TACAGCCACA GAAGGCATCT GCATAGAGAG GCCAGAGAAT 
CAGTATGTCT ACAAAATTGG CATTTATGGC TGGAGAAAGC GCTGTCTCTA CTTGTTTGTT 
CTTCTTTTAC TCATCATCCT CGTTGTGAAT TTAGCTCTTA CAATTTGGAT TCTTAAAGTG 
ATGTGGTTTT CTCCAGCAGG AATGGGCCAC TTGTGTGTAA CAAAAGATGG ACTGCGCTTG 
GAAGGGGAAT CAGAATTTTT ATTCCCATTG TATGCCAAAG AAATACACTC CAGAGTGGAC 
TCATCTCTGC TTCTACAATC AACCCAGAAT GTGACTGTAA ATGCGCGCAA CTCAGAAGGG 
GAGGTCACAG GCAGGTTAAA AGTCGGTCCC AAAATGGTAG AAGTCCAGAA TCAACAGTTT 
CAGATCAACT CCAACGACGG CAAGCCACTA TTTACTGTAG ATGAGAAGGA AGTTGTGGTT 
GGTACAGATA AACTTCGAGT AACTGGGCCT GAAGGGGCTC TTTTTGAACA TTCAGTGGAG 
ACACCCCTTG TCAGAGCCGA CCCGTTTCAA GACCTTAGAT TAGAATCCCC CACTCGGAGT 
CTAAGCATGG ATGCCCCAAG GGGTGTGCAT ATTCAAGCTC ACGCTGGGAA AATTGAGGCG 
CTTTCTCAAA TGGATATTCT TTTTCATAGT AGTGATGGAA TGCTTGTGCT TGATGCTGAA 
ACTGTGTGCT TACCCAAGCT GGTGCAGGGG ACGTGGGGTC CCTCTGGCAG CTCACAGAGC 
CTCTACGAAA TCTGTGTGTG TCCAGATGGG AAGCTGTACC TGTCTGTGGC CGGTGTGAGC 
ACCACGTGCC AGGAGCACAG CCACATCTGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000222.1
CDS	156..1031
Translation	MVREQYTTATEGICIERPENQYVYKIGIYGWRKRCLYLFVLLLLIILVVNLALTIWILKVMWFSPAGMGHLCVTKDGLRLEGESEFLFPLYAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQFQINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQDLRLESPTRSLSMDAPRGVHIQAHAGKIEALSQMDILFHSSDGMLVLDAETVCLPKLVQGTWGPSGSSQSLYEICVCPDGKLYLSVAGVSTTCQEHSHICL

Target ORF information:

RefSeq Version	NM_000231.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcoglycan gamma (SGCG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000231.2

ORF Insert Sequence:

ATGGTGCGTG AGCAGTACAC TACAGCCACA GAAGGCATCT GCATAGAGAG GCCAGAGAAT 
CAGTATGTCT ACAAAATTGG CATTTATGGC TGGAGAAAGC GCTGTCTCTA CTTGTTTGTT 
CTTCTTTTAC TCATCATCCT CGTTGTGAAT TTAGCTCTTA CAATTTGGAT TCTTAAAGTG 
ATGTGGTTTT CTCCAGCAGG AATGGGCCAC TTGTGTGTAA CAAAAGATGG ACTGCGCTTG 
GAAGGGGAAT CAGAATTTTT ATTCCCATTG TATGCCAAAG AAATACACTC CAGAGTGGAC 
TCATCTCTGC TTCTACAATC AACCCAGAAT GTGACTGTAA ATGCGCGCAA CTCAGAAGGG 
GAGGTCACAG GCAGGTTAAA AGTCGGTCCC AAAATGGTAG AAGTCCAGAA TCAACAGTTT 
CAGATCAACT CCAACGACGG CAAGCCACTA TTTACTGTAG ATGAGAAGGA AGTTGTGGTT 
GGTACAGATA AACTTCGAGT AACTGGGCCT GAAGGGGCTC TTTTTGAACA TTCAGTGGAG 
ACACCCCTTG TCAGAGCCGA CCCGTTTCAA GACCTTAGAT TAGAATCCCC CACTCGGAGT 
CTAAGCATGG ATGCCCCAAG GGGTGTGCAT ATTCAAGCTC ACGCTGGGAA AATTGAGGCG 
CTTTCTCAAA TGGATATTCT TTTTCATAGT AGTGATGGAA TGCTTGTGCT TGATGCTGAA 
ACTGTGTGCT TACCCAAGCT GGTGCAGGGG ACGTGGGGTC CCTCTGGCAG CTCACAGAGC 
CTCTACGAAA TCTGTGTGTG TCCAGATGGG AAGCTGTACC TGTCTGTGGC CGGTGTGAGC 
ACCACGTGCC AGGAGCACAG CCACATCTGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SGCG ( XM_006719861.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719924.1 Homo sapiens sarcoglycan gamma (SGCG), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu47253
Clone ID Related Accession (Same CDS sequence)	XM_006719861.3
Accession Version	XM_006719861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	gamma-sarcoglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719861.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAA ATGCAGCGGC TGTTTGCGCC CCGGGACTCC AAGAAGTACA GCAGATGGTG 
CGTGAGCAGT ACACTACAGC CACAGAAGGC ATCTGCATAG AGAGGCCAGA GAATCAGTAT 
GTCTACAAAA TTGGCATTTA TGGCTGGAGA AAGCGCTGTC TCTACTTGTT TGTTCTTCTT 
TTACTCATCA TCCTCGTTGT GAATTTAGCT CTTACAATTT GGATTCTTAA AGTGATGTGG 
TTTTCTCCAG CAGGAATGGG CCACTTGTGT GTAACAAAAG ATGGACTGCG CTTGGAAGGG 
GAATCAGAAT TTTTATTCCC ATTGTATGCC AAAGAAATAC ACTCCAGAGT GGACTCATCT 
CTGCTTCTAC AATCAACCCA GAATGTGACT GTAAATGCGC GCAACTCAGA AGGGGAGGTC 
ACAGGCAGGT TAAAAGTCGG TCCCAAAATG GTAGAAGTCC AGAATCAACA GTTTCAGATC 
AACTCCAACG ACGGCAAGCC ACTATTTACT GTAGATGAGA AGGAAGTTGT GGTTGGTACA 
GATAAACTTC GAGTAACTGG GCCTGAAGGG GCTCTTTTTG AACATTCAGT GGAGACACCC 
CTTGTCAGAG CCGACCCGTT TCAAGACCTT AGATTAGAAT CCCCCACTCG GAGTCTAAGC 
ATGGATGCCC CAAGGGGTGT GCATATTCAA GCTCACGCTG GGAAAATTGA GGCGCTTTCT 
CAAATGGATA TTCTTTTTCA TAGTAGTGAT GGAATGCTTG TGCTTGATGC TGAAACTGTG 
TGCTTACCCA AGCTGGTGCA GGGGACGTGG GGTCCCTCTG GCAGCTCACA GAGCCTCTAC 
GAAATCTGTG TGTGTCCAGA TGGGAAGCTG TACCTGTCTG TGGCCGGTGT GAGCACCACG 
TGCCAGGAGC ACAACCACAT CTGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719924.1
CDS	286..1215
Translation	MKGNAAAVCAPGLQEVQQMVREQYTTATEGICIERPENQYVYKIGIYGWRKRCLYLFVLLLLIILVVNLALTIWILKVMWFSPAGMGHLCVTKDGLRLEGESEFLFPLYAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQFQINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQDLRLESPTRSLSMDAPRGVHIQAHAGKIEALSQMDILFHSSDGMLVLDAETVCLPKLVQGTWGPSGSSQSLYEICVCPDGKLYLSVAGVSTTCQEHNHICL

Target ORF information:

RefSeq Version	XM_006719861.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcoglycan gamma (SGCG), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719861.3

ORF Insert Sequence:

ATGAAGGGAA ATGCAGCGGC TGTTTGCGCC CCGGGACTCC AAGAAGTACA GCAGATGGTG 
CGTGAGCAGT ACACTACAGC CACAGAAGGC ATCTGCATAG AGAGGCCAGA GAATCAGTAT 
GTCTACAAAA TTGGCATTTA TGGCTGGAGA AAGCGCTGTC TCTACTTGTT TGTTCTTCTT 
TTACTCATCA TCCTCGTTGT GAATTTAGCT CTTACAATTT GGATTCTTAA AGTGATGTGG 
TTTTCTCCAG CAGGAATGGG CCACTTGTGT GTAACAAAAG ATGGACTGCG CTTGGAAGGG 
GAATCAGAAT TTTTATTCCC ATTGTATGCC AAAGAAATAC ACTCCAGAGT GGACTCATCT 
CTGCTTCTAC AATCAACCCA GAATGTGACT GTAAATGCGC GCAACTCAGA AGGGGAGGTC 
ACAGGCAGGT TAAAAGTCGG TCCCAAAATG GTAGAAGTCC AGAATCAACA GTTTCAGATC 
AACTCCAACG ACGGCAAGCC ACTATTTACT GTAGATGAGA AGGAAGTTGT GGTTGGTACA 
GATAAACTTC GAGTAACTGG GCCTGAAGGG GCTCTTTTTG AACATTCAGT GGAGACACCC 
CTTGTCAGAG CCGACCCGTT TCAAGACCTT AGATTAGAAT CCCCCACTCG GAGTCTAAGC 
ATGGATGCCC CAAGGGGTGT GCATATTCAA GCTCACGCTG GGAAAATTGA GGCGCTTTCT 
CAAATGGATA TTCTTTTTCA TAGTAGTGAT GGAATGCTTG TGCTTGATGC TGAAACTGTG 
TGCTTACCCA AGCTGGTGCA GGGGACGTGG GGTCCCTCTG GCAGCTCACA GAGCCTCTAC 
GAAATCTGTG TGTGTCCAGA TGGGAAGCTG TACCTGTCTG TGGCCGGTGT GAGCACCACG 
TGCCAGGAGC ACAACCACAT CTGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SGCG ( XM_005266505.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005266562.1 Homo sapiens sarcoglycan gamma (SGCG), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu47254
Clone ID Related Accession (Same CDS sequence)	XM_005266505.2 , XM_024449397.1
Accession Version	XM_005266505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	gamma-sarcoglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005266505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGTG AGCAGTACAC TACAGCCACA GAAGGCATCT GCATAGAGAG GCCAGAGAAT 
CAGTATGTCT ACAAAATTGG CATTTATGGC TGGAGAAAGC GCTGTCTCTA CTTGTTTGTT 
CTTCTTTTAC TCATCATCCT CGTTGTGAAT TTAGCTCTTA CAATTTGGAT TCTTAAAGTG 
ATGTGGTTTT CTCCAGCAGG AATGGGCCAC TTGTGTGTAA CAAAAGATGG ACTGCGCTTG 
GAAGGGGAAT CAGAATTTTT ATTCCCATTG TATGCCAAAG AAATACACTC CAGAGTGGAC 
TCATCTCTGC TTCTACAATC AACCCAGAAT GTGACTGTAA ATGCGCGCAA CTCAGAAGGG 
GAGGTCACAG GCAGGTTAAA AGTCGGTCCC AAAATGGTAG AAGTCCAGAA TCAACAGTTT 
CAGATCAACT CCAACGACGG CAAGCCACTA TTTACTGTAG ATGAGAAGGA AGTTGTGGTT 
GGTACAGATA AACTTCGAGT AACTGGGCCT GAAGGGGCTC TTTTTGAACA TTCAGTGGAG 
ACACCCCTTG TCAGAGCCGA CCCGTTTCAA GACCTTAGAT TAGAATCCCC CACTCGGAGT 
CTAAGCATGG ATGCCCCAAG GGGTGTGCAT ATTCAAGCTC ACGCTGGGAA AATTGAGGCG 
CTTTCTCAAA TGGATATTCT TTTTCATAGT AGTGATGGAA TGCTTGTGCT TGATGCTGAA 
ACTGTGTGCT TACCCAAGCT GGTGCAGGGG ACGTGGGGTC CCTCTGGCAG CTCACAGAGC 
CTCTACGAAA TCTGTGTGTG TCCAGATGGG AAGCTGTACC TGTCTGTGGC CGGTGTGAGC 
ACCACGTGCC AGGAGCACAA CCACATCTGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005266562.1
CDS	249..1124
Translation	MVREQYTTATEGICIERPENQYVYKIGIYGWRKRCLYLFVLLLLIILVVNLALTIWILKVMWFSPAGMGHLCVTKDGLRLEGESEFLFPLYAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQFQINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQDLRLESPTRSLSMDAPRGVHIQAHAGKIEALSQMDILFHSSDGMLVLDAETVCLPKLVQGTWGPSGSSQSLYEICVCPDGKLYLSVAGVSTTCQEHNHICL

Target ORF information:

RefSeq Version	XM_005266505.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcoglycan gamma (SGCG), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005266505.2

ORF Insert Sequence:

ATGGTGCGTG AGCAGTACAC TACAGCCACA GAAGGCATCT GCATAGAGAG GCCAGAGAAT 
CAGTATGTCT ACAAAATTGG CATTTATGGC TGGAGAAAGC GCTGTCTCTA CTTGTTTGTT 
CTTCTTTTAC TCATCATCCT CGTTGTGAAT TTAGCTCTTA CAATTTGGAT TCTTAAAGTG 
ATGTGGTTTT CTCCAGCAGG AATGGGCCAC TTGTGTGTAA CAAAAGATGG ACTGCGCTTG 
GAAGGGGAAT CAGAATTTTT ATTCCCATTG TATGCCAAAG AAATACACTC CAGAGTGGAC 
TCATCTCTGC TTCTACAATC AACCCAGAAT GTGACTGTAA ATGCGCGCAA CTCAGAAGGG 
GAGGTCACAG GCAGGTTAAA AGTCGGTCCC AAAATGGTAG AAGTCCAGAA TCAACAGTTT 
CAGATCAACT CCAACGACGG CAAGCCACTA TTTACTGTAG ATGAGAAGGA AGTTGTGGTT 
GGTACAGATA AACTTCGAGT AACTGGGCCT GAAGGGGCTC TTTTTGAACA TTCAGTGGAG 
ACACCCCTTG TCAGAGCCGA CCCGTTTCAA GACCTTAGAT TAGAATCCCC CACTCGGAGT 
CTAAGCATGG ATGCCCCAAG GGGTGTGCAT ATTCAAGCTC ACGCTGGGAA AATTGAGGCG 
CTTTCTCAAA TGGATATTCT TTTTCATAGT AGTGATGGAA TGCTTGTGCT TGATGCTGAA 
ACTGTGTGCT TACCCAAGCT GGTGCAGGGG ACGTGGGGTC CCTCTGGCAG CTCACAGAGC 
CTCTACGAAA TCTGTGTGTG TCCAGATGGG AAGCTGTACC TGTCTGTGGC CGGTGTGAGC 
ACCACGTGCC AGGAGCACAA CCACATCTGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SGCG ( XM_024449397.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305165.1 Homo sapiens sarcoglycan gamma (SGCG), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu47254
Clone ID Related Accession (Same CDS sequence)	XM_005266505.2 , XM_024449397.1
Accession Version	XM_024449397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	gamma-sarcoglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGTG AGCAGTACAC TACAGCCACA GAAGGCATCT GCATAGAGAG GCCAGAGAAT 
CAGTATGTCT ACAAAATTGG CATTTATGGC TGGAGAAAGC GCTGTCTCTA CTTGTTTGTT 
CTTCTTTTAC TCATCATCCT CGTTGTGAAT TTAGCTCTTA CAATTTGGAT TCTTAAAGTG 
ATGTGGTTTT CTCCAGCAGG AATGGGCCAC TTGTGTGTAA CAAAAGATGG ACTGCGCTTG 
GAAGGGGAAT CAGAATTTTT ATTCCCATTG TATGCCAAAG AAATACACTC CAGAGTGGAC 
TCATCTCTGC TTCTACAATC AACCCAGAAT GTGACTGTAA ATGCGCGCAA CTCAGAAGGG 
GAGGTCACAG GCAGGTTAAA AGTCGGTCCC AAAATGGTAG AAGTCCAGAA TCAACAGTTT 
CAGATCAACT CCAACGACGG CAAGCCACTA TTTACTGTAG ATGAGAAGGA AGTTGTGGTT 
GGTACAGATA AACTTCGAGT AACTGGGCCT GAAGGGGCTC TTTTTGAACA TTCAGTGGAG 
ACACCCCTTG TCAGAGCCGA CCCGTTTCAA GACCTTAGAT TAGAATCCCC CACTCGGAGT 
CTAAGCATGG ATGCCCCAAG GGGTGTGCAT ATTCAAGCTC ACGCTGGGAA AATTGAGGCG 
CTTTCTCAAA TGGATATTCT TTTTCATAGT AGTGATGGAA TGCTTGTGCT TGATGCTGAA 
ACTGTGTGCT TACCCAAGCT GGTGCAGGGG ACGTGGGGTC CCTCTGGCAG CTCACAGAGC 
CTCTACGAAA TCTGTGTGTG TCCAGATGGG AAGCTGTACC TGTCTGTGGC CGGTGTGAGC 
ACCACGTGCC AGGAGCACAA CCACATCTGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305165.1
CDS	194..1069
Translation	MVREQYTTATEGICIERPENQYVYKIGIYGWRKRCLYLFVLLLLIILVVNLALTIWILKVMWFSPAGMGHLCVTKDGLRLEGESEFLFPLYAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQFQINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQDLRLESPTRSLSMDAPRGVHIQAHAGKIEALSQMDILFHSSDGMLVLDAETVCLPKLVQGTWGPSGSSQSLYEICVCPDGKLYLSVAGVSTTCQEHNHICL

Target ORF information:

RefSeq Version	XM_024449397.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sarcoglycan gamma (SGCG), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449397.1

ORF Insert Sequence:

ATGGTGCGTG AGCAGTACAC TACAGCCACA GAAGGCATCT GCATAGAGAG GCCAGAGAAT 
CAGTATGTCT ACAAAATTGG CATTTATGGC TGGAGAAAGC GCTGTCTCTA CTTGTTTGTT 
CTTCTTTTAC TCATCATCCT CGTTGTGAAT TTAGCTCTTA CAATTTGGAT TCTTAAAGTG 
ATGTGGTTTT CTCCAGCAGG AATGGGCCAC TTGTGTGTAA CAAAAGATGG ACTGCGCTTG 
GAAGGGGAAT CAGAATTTTT ATTCCCATTG TATGCCAAAG AAATACACTC CAGAGTGGAC 
TCATCTCTGC TTCTACAATC AACCCAGAAT GTGACTGTAA ATGCGCGCAA CTCAGAAGGG 
GAGGTCACAG GCAGGTTAAA AGTCGGTCCC AAAATGGTAG AAGTCCAGAA TCAACAGTTT 
CAGATCAACT CCAACGACGG CAAGCCACTA TTTACTGTAG ATGAGAAGGA AGTTGTGGTT 
GGTACAGATA AACTTCGAGT AACTGGGCCT GAAGGGGCTC TTTTTGAACA TTCAGTGGAG 
ACACCCCTTG TCAGAGCCGA CCCGTTTCAA GACCTTAGAT TAGAATCCCC CACTCGGAGT 
CTAAGCATGG ATGCCCCAAG GGGTGTGCAT ATTCAAGCTC ACGCTGGGAA AATTGAGGCG 
CTTTCTCAAA TGGATATTCT TTTTCATAGT AGTGATGGAA TGCTTGTGCT TGATGCTGAA 
ACTGTGTGCT TACCCAAGCT GGTGCAGGGG ACGTGGGGTC CCTCTGGCAG CTCACAGAGC 
CTCTACGAAA TCTGTGTGTG TCCAGATGGG AAGCTGTACC TGTCTGTGGC CGGTGTGAGC 
ACCACGTGCC AGGAGCACAA CCACATCTGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-sarcoglycan and dystrophin mutation spectrum in an Algerian cohort.
Muscle & nerve56(1)129-135(2017 Jul)
Dalichaouche I,Sifi Y,Roudaut C,Sifi K,Hamri A,Rouabah L,Abadi N,Richard I

Crystal Structures of SgcE6 and SgcC, the Two-Component Monooxygenase That Catalyzes Hydroxylation of a Carrier Protein-Tethered Substrate during the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027 in Streptomyces globisporus.
Biochemistry55(36)5142-54(2016 09)
Chang CY,Lohman JR,Cao H,Tan K,Rudolf JD,Ma M,Xu W,Bingman CA,Yennamalli RM,Bigelow L,Babnigg G,Yan X,Joachimiak A,Phillips GN Jr,Shen B

Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle.
Human molecular genetics24(9)2470-81(2015 May)
Spinazzola JM,Smith TC,Liu M,Luna EJ,Barton ER

Carrier frequency of the c.525delT mutation in the SGCG gene and estimated prevalence of limb girdle muscular dystrophy type 2C among the Moroccan population.
Genetic testing and molecular biomarkers18(4)253-6(2014 Apr)
El Kerch F,Ratbi I,Sbiti A,Laarabi FZ,Barkat A,Sefiani A

Puerto Rican founder mutation G787A in the SGCG gene: a case report of 2 siblings with LGMD 2C.
Journal of clinical neuromuscular disease15(3)105-7(2014 Mar)
DiCapua D,Patwa H