TK1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following TK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu08944 NM_003258.5
Latest version!
Homo sapiens thymidine kinase 1 (TK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$134.50-$188.30
$269.00
OHu100746 NM_001346663.2
Latest version!
Homo sapiens thymidine kinase 1 (TK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu107130 NM_001363848.1
Latest version!
Homo sapiens thymidine kinase 1 (TK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu87437 XM_017024992.1
Latest version!
Homo sapiens thymidine kinase 1 (TK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu08944 NM_003258.4 Homo sapiens thymidine kinase 1 (TK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$134.50-$188.30
$269.00
OHu100746 NM_001346663.1 Homo sapiens thymidine kinase 1 (TK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu08944
    Clone ID Related Accession (Same CDS sequence) NM_003258.5 , NM_003258.4
    Accession Version NM_003258.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product thymidine kinase, cytosolic isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI754079.1 and BC006484.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003258.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.457995.1, SRR5189658.163613.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9575153) MANE Ensembl match :: ENST00000301634.12/ ENSP00000301634.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGGTCGAGG TGATTGGGGG AGCAGACAAG
    TACCACTCCG TGTGTCGGCT CTGCTACTTC AAGAAGGCCT CAGGCCAGCC TGCCGGGCCG
    GACAACAAAG AGAACTGCCC AGTGCCAGGA AAGCCAGGGG AAGCCGTGGC TGCCAGGAAG
    CTCTTTGCCC CACAGCAGAT TCTGCAATGC AGCCCTGCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003249.3
    CDS59..763
    Translation

    Target ORF information:

    RefSeq Version NM_003258.5
    Organism Homo sapiens(human)
    Definition Homo sapiens thymidine kinase 1 (TK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003258.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGGTCGAGG TGATTGGGGG AGCAGACAAG
    TACCACTCCG TGTGTCGGCT CTGCTACTTC AAGAAGGCCT CAGGCCAGCC TGCCGGGCCG
    GACAACAAAG AGAACTGCCC AGTGCCAGGA AAGCCAGGGG AAGCCGTGGC TGCCAGGAAG
    CTCTTTGCCC CACAGCAGAT TCTGCAATGC AGCCCTGCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08944
    Clone ID Related Accession (Same CDS sequence) NM_003258.5 , NM_003258.4
    Accession Version NM_003258.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product thymidine kinase, cytosolic isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI754079.1, BC006484.1 and AA633286.1. This sequence is a reference standard in the RefSeqGene project. On Jan 15, 2008 this sequence version replaced NM_003258.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.509094.1, SRR1163658.563798.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9575153) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGGTCGAGG TGATTGGGGG AGCAGACAAG
    TACCACTCCG TGTGTCGGCT CTGCTACTTC AAGAAGGCCT CAGGCCAGCC TGCCGGGCCG
    GACAACAAAG AGAACTGCCC AGTGCCAGGA AAGCCAGGGG AAGCCGTGGC TGCCAGGAAG
    CTCTTTGCCC CACAGCAGAT TCTGCAATGC AGCCCTGCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003249.3
    CDS211..915
    Translation

    Target ORF information:

    RefSeq Version NM_003258.4
    Organism Homo sapiens(human)
    Definition Homo sapiens thymidine kinase 1 (TK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003258.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGGTCGAGG TGATTGGGGG AGCAGACAAG
    TACCACTCCG TGTGTCGGCT CTGCTACTTC AAGAAGGCCT CAGGCCAGCC TGCCGGGCCG
    GACAACAAAG AGAACTGCCC AGTGCCAGGA AAGCCAGGGG AAGCCGTGGC TGCCAGGAAG
    CTCTTTGCCC CACAGCAGAT TCTGCAATGC AGCCCTGCCA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100746
    Clone ID Related Accession (Same CDS sequence) NM_001346663.1 , NM_001346663.2
    Accession Version NM_001346663.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product thymidine kinase, cytosolic isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087645.19 and AC021593.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.95509.1, SRR1163657.469647.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9575153) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGCAGATTC TGCAATGCAG CCCTGCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333592.1
    CDS211..753
    Translation

    Target ORF information:

    RefSeq Version NM_001346663.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thymidine kinase 1 (TK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346663.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGCAGATTC TGCAATGCAG CCCTGCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100746
    Clone ID Related Accession (Same CDS sequence) NM_001346663.1 , NM_001346663.2
    Accession Version NM_001346663.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product thymidine kinase, cytosolic isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087645.19 and AC021593.15. On May 31, 2019 this sequence version replaced NM_001346663.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.469647.1, SRR1163657.95509.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9575153) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGCAGATTC TGCAATGCAG CCCTGCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333592.1
    CDS59..601
    Translation

    Target ORF information:

    RefSeq Version NM_001346663.2
    Organism Homo sapiens(human)
    Definition Homo sapiens thymidine kinase 1 (TK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346663.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGCAGATTC TGCAATGCAG CCCTGCCAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107130
    Clone ID Related Accession (Same CDS sequence) NM_001363848.1
    Accession Version NM_001363848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product thymidine kinase, cytosolic isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087645.19 and AC021593.15. On Jun 3, 2018 this sequence version replaced XM_017024992.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.479174.1, SRR1163658.230355.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9575153) ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGGTAGCTC CACCTGCCTT CCCTGCAGGC
    CGGCGGGGTG GGGGTATGGC TCTGCCTCCT TCCTGTCCTG GCCCTTCACC CATCCCCTGT
    CCCTGCGGCC AGGTCGAGGT GATTGGGGGA GCAGACAAGT ACCACTCCGT GTGTCGGCTC
    TGCTACTTCA AGAAGGCCTC AGGCCAGCCT GCCGGGCCGG ACAACAAAGA GAACTGCCCA
    GTGCCAGGAA AGCCAGGGGA AGCCGTGGCT GCCAGGAAGC TCTTTGCCCC ACAGCAGATT
    CTGCAATGCA GCCCTGCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350777.1
    CDS211..1014
    Translation

    Target ORF information:

    RefSeq Version NM_001363848.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thymidine kinase 1 (TK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAGCTGCA TTAACCTGCC CACTGTGCTG CCTGGCTCCC CCAGCAAGAC CCGGGGGCAG 
    ATCCAGGTGA TTCTCGGGCC GATGTTCTCA GGAAAAAGCA CAGAGTTGAT GAGACGCGTC
    CGTCGCTTCC AGATTGCTCA GTACAAGTGC CTGGTGATCA AGTATGCCAA AGACACTCGC
    TACAGCAGCA GCTTCTGCAC ACATGACCGG AACACCATGG AGGCACTGCC CGCCTGCCTG
    CTCCGAGACG TGGCCCAGGA GGCCCTGGGC GTGGCTGTCA TAGGCATCGA CGAGGGGCAG
    TTTTTCCCTG ACATCGTGGA GTTCTGCGAG GCCATGGCCA ACGCCGGGAA GACCGTAATT
    GTGGCTGCAC TGGATGGGAC CTTCCAGAGG AAGCCATTTG GGGCCATCCT GAACCTGGTG
    CCGCTGGCCG AGAGCGTGGT GAAGCTGACG GCGGTGTGCA TGGAGTGCTT CCGGGAAGCC
    GCCTATACCA AGAGGCTCGG CACAGAGAAG GAGGTAGCTC CACCTGCCTT CCCTGCAGGC
    CGGCGGGGTG GGGGTATGGC TCTGCCTCCT TCCTGTCCTG GCCCTTCACC CATCCCCTGT
    CCCTGCGGCC AGGTCGAGGT GATTGGGGGA GCAGACAAGT ACCACTCCGT GTGTCGGCTC
    TGCTACTTCA AGAAGGCCTC AGGCCAGCCT GCCGGGCCGG ACAACAAAGA GAACTGCCCA
    GTGCCAGGAA AGCCAGGGGA AGCCGTGGCT GCCAGGAAGC TCTTTGCCCC ACAGCAGATT
    CTGCAATGCA GCCCTGCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87437
    Clone ID Related Accession (Same CDS sequence) XM_017024992.1
    Accession Version XM_017024992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product thymidine kinase, cytosolic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGGCGG GGCCGGCTCG TGATTGGCCA GCACGCCGTG GTTTAAAGCG GTCGGCGCGG 
    GAACCAGGGG CTTACTGCGG GACGGCCTTG GAGAGTACTC GGGTTCGTGA ACTTCCCGGA
    GGCGCAATGA GCTGCATTAA CCTGCCCACT GTGCTGCCTG GCTCCCCCAG CAAGACCCGG
    GGGCAGATCC AGGTGATTCT CGGGCCGATG TTCTCAGGAA AAAGCACAGA GTTGATGAGA
    CGCGTCCGTC GCTTCCAGAT TGCTCAGTAC AAGTGCCTGG TGATCAAGTA TGCCAAAGAC
    ACTCGCTACA GCAGCAGCTT CTGCACACAT GACCGGAACA CCATGGAGGC ACTGCCCGCC
    TGCCTGCTCC GAGACGTGGC CCAGGAGGCC CTGGGCGTGG CTGTCATAGG CATCGACGAG
    GGGCAGTTTT TCCCTGACAT CGTGGAGTTC TGCGAGGCCA TGGCCAACGC CGGGAAGACC
    GTAATTGTGG CTGCACTGGA TGGGACCTTC CAGAGGAAGC CATTTGGGGC CATCCTGAAC
    CTGGTGCCGC TGGCCGAGAG CGTGGTGAAG CTGACGGCGG TGTGCATGGA GTGCTTCCGG
    GAAGCCGCCT ATACCAAGAG GCTCGGCACA GAGAAGGAGG TAGCTCCACC TGCCTTCCCT
    GCAGGCCGGC GGGGTGGGGG TATGGCTCTG CCTCCTTCCT GTCCTGGCCC TTCACCCATC
    CCCTGTCCCT GCGGCCAGGT CGAGGTGATT GGGGGAGCAG ACAAGTACCA CTCCGTGTGT
    CGGCTCTGCT ACTTCAAGAA GGCCTCAGGC CAGCCTGCCG GGCCGGACAA CAAAGAGAAC
    TGCCCAGTGC CAGGAAAGCC AGGGGAAGCC GTGGCTGCCA GGAAGCTCTT TGCCCCACAG
    CAGATTCTGC AATGCAGCCC TGCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880481.1
    CDS89..1018
    Translation

    Target ORF information:

    RefSeq Version XM_017024992.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thymidine kinase 1 (TK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGGCGG GGCCGGCTCG TGATTGGCCA GCACGCCGTG GTTTAAAGCG GTCGGCGCGG 
    GAACCAGGGG CTTACTGCGG GACGGCCTTG GAGAGTACTC GGGTTCGTGA ACTTCCCGGA
    GGCGCAATGA GCTGCATTAA CCTGCCCACT GTGCTGCCTG GCTCCCCCAG CAAGACCCGG
    GGGCAGATCC AGGTGATTCT CGGGCCGATG TTCTCAGGAA AAAGCACAGA GTTGATGAGA
    CGCGTCCGTC GCTTCCAGAT TGCTCAGTAC AAGTGCCTGG TGATCAAGTA TGCCAAAGAC
    ACTCGCTACA GCAGCAGCTT CTGCACACAT GACCGGAACA CCATGGAGGC ACTGCCCGCC
    TGCCTGCTCC GAGACGTGGC CCAGGAGGCC CTGGGCGTGG CTGTCATAGG CATCGACGAG
    GGGCAGTTTT TCCCTGACAT CGTGGAGTTC TGCGAGGCCA TGGCCAACGC CGGGAAGACC
    GTAATTGTGG CTGCACTGGA TGGGACCTTC CAGAGGAAGC CATTTGGGGC CATCCTGAAC
    CTGGTGCCGC TGGCCGAGAG CGTGGTGAAG CTGACGGCGG TGTGCATGGA GTGCTTCCGG
    GAAGCCGCCT ATACCAAGAG GCTCGGCACA GAGAAGGAGG TAGCTCCACC TGCCTTCCCT
    GCAGGCCGGC GGGGTGGGGG TATGGCTCTG CCTCCTTCCT GTCCTGGCCC TTCACCCATC
    CCCTGTCCCT GCGGCCAGGT CGAGGTGATT GGGGGAGCAG ACAAGTACCA CTCCGTGTGT
    CGGCTCTGCT ACTTCAAGAA GGCCTCAGGC CAGCCTGCCG GGCCGGACAA CAAAGAGAAC
    TGCCCAGTGC CAGGAAAGCC AGGGGAAGCC GTGGCTGCCA GGAAGCTCTT TGCCCCACAG
    CAGATTCTGC AATGCAGCCC TGCCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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