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TLE1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TLE1
Entrez Gene ID 7088
Full Name transducin like enhancer of split 1
Synonyms ESG,ESG1,GRG1
General protein information
Preferred Names

transducin like enhancer of split 1

Names

transducin-like enhancer protein 1
enhancer of split groucho-like protein 1
transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

9

9q21.32

Disorder MIM:

600189


mRNA and Protein(s)

mRNA Protein Name
NM_001303104.1 NP_001290033.1 transducin-like enhancer protein 1 isoform 3
NM_001303103.1 NP_001290032.1 transducin-like enhancer protein 1 isoform 1
XM_005252151.1 XP_005252208.1 transducin-like enhancer protein 1 isoform X1
XM_005252153.1 XP_005252210.1 transducin-like enhancer protein 1 isoform X4
XM_006717262.1 XP_006717325.1 transducin-like enhancer protein 1 isoform X9
XM_006717263.1 XP_006717326.1 transducin-like enhancer protein 1 isoform X17
XM_005252154.1 XP_005252211.1 transducin-like enhancer protein 1 isoform X5
XM_006717260.1 XP_006717323.1 transducin-like enhancer protein 1 isoform X12
XM_006717261.2 XP_006717324.1 transducin-like enhancer protein 1 isoform X15
NM_005077.4 NP_005068.2 transducin-like enhancer protein 1 isoform 2
XM_017015065.1 XP_016870554.1 transducin-like enhancer protein 1 isoform X8
XM_011518951.2 XP_011517253.1 transducin-like enhancer protein 1 isoform X10
XM_005252162.1 XP_005252219.1 transducin-like enhancer protein 1 isoform X13
XM_017015066.1 XP_016870555.1 transducin-like enhancer protein 1 isoform X14
XM_017015064.1 XP_016870553.1 transducin-like enhancer protein 1 isoform X7
XM_005252152.1 XP_005252209.1 transducin-like enhancer protein 1 isoform X2
XM_006717258.1 XP_006717321.1 transducin-like enhancer protein 1 isoform X3
XM_005252156.2 XP_005252213.1 transducin-like enhancer protein 1 isoform X6
XM_006717259.3 XP_006717322.1 transducin-like enhancer protein 1 isoform X11
XM_005252163.2 XP_005252220.1 transducin-like enhancer protein 1 isoform X16



Homo sapiens (human) TLE1 NP_005068.2
Gallus gallus (chicken) TLE1 XP_004949238.1
Rattus norvegicus (Norway rat) Tle1 NP_001166904.1
Canis lupus familiaris (dog) TLE1 XP_533516.3
Mus musculus (house mouse) Tle1 NP_035729.3
Pan troglodytes (chimpanzee) LOC464546 XP_003312170.1
Xenopus tropicalis (tropical clawed frog) tle1 NP_001163974.1
Macaca mulatta (rhesus monkey) TLE1 XP_001104021.1


Related articles in PubMed

Transducin-like enhancer of split-1 is expressed and functional in human macrophages.
De Paoli F, Copin C, Vanhoutte J, Derudas B, Vinod M, Zawadzki C, Susen S, Pattou F, Haulon S, Staels B, Eeckhoute J, Chinetti-Gbaguidi G
FEBS letters590(1)43-52(2016 Jan)

TLE1 promotes EMT in A549 lung cancer cells through suppression of E-cadherin.
Yao X, Ireland SK, Pham T, Temple B, Chen R, Raj MH, Biliran H
Biochemical and biophysical research communications455(3-4)277-84(2014 Dec)

Biological insights from 108 schizophrenia-associated genetic loci.

Nature511(7510)421-7(2014 Jul)

Grg3/TLE3 and Grg1/TLE1 induce monohormonal pancreatic β-cells while repressing α-cell functions.
Metzger DE, Liu C, Ziaie AS, Naji A, Zaret KS
Diabetes63(5)1804-16(2014 May)

Molecular functions of the TLE tetramerization domain in Wnt target gene repression.
Chodaparambil JV, Pate KT, Hepler MR, Tsai BP, Muthurajan UM, Luger K, Waterman ML, Weis WI
The EMBO journal33(7)719-31(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TLE1 is not a stand-alone diagnostic immunohistochemical marker for synovial sarcoma
Title: Transducer-like enhancer of split 1 (TLE1) expression as a diagnostic immunohistochemical marker for synovial sarcoma and its association with morphological features.

Data suggest TLE1 is highly expressed in macrophages cultured in vitro, in macrophages of atherosclerotic plaques, and in macrophages of visceral adipose tissue from obese women; expression of TLE1 is up-regulated upon macrophage activation.
Title: Transducin-like enhancer of split-1 is expressed and functional in human macrophages.

TLE1 has a role in regulating epithelial-to-mesenchymal transition in A549 cells through its repressive effect on E-cadherin
Title: TLE1 promotes EMT in A549 lung cancer cells through suppression of E-cadherin.

GrG1 is involved in pancreatic cell differentiation, establishing a monohormonal beta cell identity. Grg1 is the predominant Groucho expressed in human beta-cells but acts functionally similarly to Grg3.
Title: Grg3/TLE3 and Grg1/TLE1 induce monohormonal pancreatic β-cells while repressing α-cell functions.

TCF/TLE tetramer complex promotes structural transitions of chromatin to mediate repression.
Title: Molecular functions of the TLE tetramerization domain in Wnt target gene repression.

The following TLE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu43471 NM_001303103.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu61006 NM_005077.4
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu61007 NM_001303104.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43466 XM_005252151.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43467 XM_005252152.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43468 XM_006717258.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43469 XM_005252153.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43470 XM_005252154.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43472 XM_005252156.2
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43473 XM_006717259.3
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43474 XM_006717260.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43475 XM_005252162.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43476 XM_005252163.2
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43477 XM_006717261.2
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43478 XM_006717262.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu43479 XM_006717263.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu61005 XM_011518951.2
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu98859 XM_017015064.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu98860 XM_017015065.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu98861 XM_017015066.1
Latest version!
Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu43471
Clone ID Related Accession (Same CDS sequence) NM_001303103.1
Accession Version NM_001303103.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB209854.1, BC000228.1, DB211613.1, AL353682.11 and BC010100.2. On Dec 17, 2014 this sequence version replaced XM_005252155.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000228.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGCAGCTGG CTTGAGGACA CCCCTGGCAG TGCCCGGCCC ATATCCTGCT
CCTTTTGGGA TGGTCCCCCA CGCTGGCATG AACGGCGAGC TGACCAGCCC AGGCGCTGCC
TACGCCAGTT TACACAACAT GTCGCCCCAG ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG
GTGGCCTACG GGCGCTCCCC CATGGTGGGG TTTGATCCTC CCCCTCACAT GAGAGTACCT
ACCATTCCTC CAAACCTGGC AGGAATCCCT GGGGGGAAAC CTGCATACTC CTTCCACGTT
ACTGCAGACG GTCAGATGCA GCCTGTCCCT TTTCCCCCCG ACGCCCTCAT CGGACCCGGA
ATCCCCCGGC ATGCTCGCCA GATCAACACC CTCAACCACG GGGAGGTGGT GTGCGCTGTG
ACCATCAGCA ACCCCACGAG ACACGTGTAC ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG
GACATCAGCC ACCCTGGCAA TAAGAGCCCT GTCTCCCAGC TCGACTGTCT GAACAGAGAC
AATTATATCC GTTCCTGTAA ATTGCTACCC GATGGCTGCA CTCTCATAGT GGGAGGGGAA
GCCAGTACTT TGTCCATTTG GGACCTGGCG GCTCCAACCC CGCGCATCAA GGCGGAGCTG
ACGTCCTCGG CCCCCGCCTG CTACGCCCTG GCCATCAGCC CCGATTCCAA GGTCTGCTTC
TCATGCTGCA GCGACGGCAA CATCGCTGTG TGGGATCTGC ACAACCAGAC ACTAGTGAGG
CAATTCCAGG GCCACACAGA CGGAGCCAGC TGTATTGACA TTTCTAATGA TGGCACCAAG
CTCTGGACGG GTGGTTTGGA CAACACAGTC AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG
CTGCAGCAGC ACGACTTCAC CTCCCAGATC TTCTCCCTGG GGTACTGCCC CACCGGGGAG
TGGCTGGCAG TGGGCATGGA GAGCAGCAAT GTGGAGGTGC TGCACGTGAA CAAGCCTGAC
AAGTACCAGC TGCACCTGCA TGAGAGCTGC GTGCTGTCCC TGAAATTTGC TTACTGTGGT
AAATGGTTTG TGAGTACTGG AAAAGATAAC CTCCTCAATG CTTGGCGGAC CCCCTATGGA
GCCAGCATAT TCCAGTCCAA AGAGTCCTCG TCAGTGCTTA GCTGTGACAT CTCTGTGGAT
GATAAGTACA TAGTCACTGG CTCGGGGGAC AAGAAGGCTA CAGTCTATGA AGTCATCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001290032.1
CDS1296..3638
Misc Feature(1)1212..1214
Misc Feature(2)1296..1724
Misc Feature(3)1719..1922
Misc Feature(4)1923..2129
Misc Feature(5)1998..2009
Misc Feature(6)2040..2042
Misc Feature(7)2100..2102
Misc Feature(8)2112..2114
Misc Feature(9)2124..2126
Misc Feature(10)2130..2675
Misc Feature(11)2181..2183
Misc Feature(12)2733..2828
Misc Feature(13)<2751..3629
Misc Feature(14)2772..3626
Misc Feature(15)order(2772..2774,2826..2828,2853..2858,2904..2909,2964..2966,2976..2978,2994..2999,3039..3044,3093..3095,3108..3110,3126..3131,3168..3170,3219..3221,3234..3236,3252..3257,3282..3287,3345..3347,3357..3359,3375..3380,3411..3416,3465..3467,3480..3482,3498..3503,3534..3539,3591..3593,3603..3605,3621..3626)
Misc Feature(16)2784..2903
Misc Feature(17)2907..2999
Misc Feature(18)2925..3041
Misc Feature(19)3039..3131
Misc Feature(20)3054..3164
Misc Feature(21)3165..3257
Misc Feature(22)3183..3290
Misc Feature(23)3303..3404
Misc Feature(24)3411..3503
Misc Feature(25)3426..3530
Misc Feature(26)3534..3626
Misc Feature(27)3549..3623
Exon (1)1..1319
Exon (2)1320..1420
Exon (3)1421..1484
Exon (4)1485..1529
Exon (5)1530..1592
Exon (6)1593..1697
Exon (7)1698..1902
Exon (8)1903..1919
Exon (9)1920..2036
Exon (10)2037..2090
Exon (11)2091..2243
Exon (12)2244..2388
Exon (13)2389..2579
Exon (14)2580..2656
Exon (15)2657..2906
Exon (16)2907..3154
Exon (17)3155..3302
Exon (18)3303..3453
Exon (19)3454..3530
Exon (20)3531..4153
Translation

Target ORF information:

RefSeq Version NM_001303103.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001303103.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGCAGCTGG CTTGAGGACA CCCCTGGCAG TGCCCGGCCC ATATCCTGCT
CCTTTTGGGA TGGTCCCCCA CGCTGGCATG AACGGCGAGC TGACCAGCCC AGGCGCTGCC
TACGCCAGTT TACACAACAT GTCGCCCCAG ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG
GTGGCCTACG GGCGCTCCCC CATGGTGGGG TTTGATCCTC CCCCTCACAT GAGAGTACCT
ACCATTCCTC CAAACCTGGC AGGAATCCCT GGGGGGAAAC CTGCATACTC CTTCCACGTT
ACTGCAGACG GTCAGATGCA GCCTGTCCCT TTTCCCCCCG ACGCCCTCAT CGGACCCGGA
ATCCCCCGGC ATGCTCGCCA GATCAACACC CTCAACCACG GGGAGGTGGT GTGCGCTGTG
ACCATCAGCA ACCCCACGAG ACACGTGTAC ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG
GACATCAGCC ACCCTGGCAA TAAGAGCCCT GTCTCCCAGC TCGACTGTCT GAACAGAGAC
AATTATATCC GTTCCTGTAA ATTGCTACCC GATGGCTGCA CTCTCATAGT GGGAGGGGAA
GCCAGTACTT TGTCCATTTG GGACCTGGCG GCTCCAACCC CGCGCATCAA GGCGGAGCTG
ACGTCCTCGG CCCCCGCCTG CTACGCCCTG GCCATCAGCC CCGATTCCAA GGTCTGCTTC
TCATGCTGCA GCGACGGCAA CATCGCTGTG TGGGATCTGC ACAACCAGAC ACTAGTGAGG
CAATTCCAGG GCCACACAGA CGGAGCCAGC TGTATTGACA TTTCTAATGA TGGCACCAAG
CTCTGGACGG GTGGTTTGGA CAACACAGTC AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG
CTGCAGCAGC ACGACTTCAC CTCCCAGATC TTCTCCCTGG GGTACTGCCC CACCGGGGAG
TGGCTGGCAG TGGGCATGGA GAGCAGCAAT GTGGAGGTGC TGCACGTGAA CAAGCCTGAC
AAGTACCAGC TGCACCTGCA TGAGAGCTGC GTGCTGTCCC TGAAATTTGC TTACTGTGGT
AAATGGTTTG TGAGTACTGG AAAAGATAAC CTCCTCAATG CTTGGCGGAC CCCCTATGGA
GCCAGCATAT TCCAGTCCAA AGAGTCCTCG TCAGTGCTTA GCTGTGACAT CTCTGTGGAT
GATAAGTACA TAGTCACTGG CTCGGGGGAC AAGAAGGCTA CAGTCTATGA AGTCATCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61006
Clone ID Related Accession (Same CDS sequence) NM_005077.4
Accession Version NM_005077.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB209854.1, BC010100.2 and DB211613.1. On Dec 17, 2014 this sequence version replaced NM_005077.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010100.2, AK290860.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT
CTCGGCAAGC CTCCAGCCAT AGACCCCCTC GTTAACCAAG CGGCAGCTGG CTTGAGGACA
CCCCTGGCAG TGCCCGGCCC ATATCCTGCT CCTTTTGGGA TGGTCCCCCA CGCTGGCATG
AACGGCGAGC TGACCAGCCC AGGCGCTGCC TACGCCAGTT TACACAACAT GTCGCCCCAG
ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG GTGGCCTACG GGCGCTCCCC CATGGTGGGG
TTTGATCCTC CCCCTCACAT GAGAGTACCT ACCATTCCTC CAAACCTGGC AGGAATCCCT
GGGGGGAAAC CTGCATACTC CTTCCACGTT ACTGCAGACG GTCAGATGCA GCCTGTCCCT
TTTCCCCCCG ACGCCCTCAT CGGACCCGGA ATCCCCCGGC ATGCTCGCCA GATCAACACC
CTCAACCACG GGGAGGTGGT GTGCGCTGTG ACCATCAGCA ACCCCACGAG ACACGTGTAC
ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG GACATCAGCC ACCCTGGCAA TAAGAGCCCT
GTCTCCCAGC TCGACTGTCT GAACAGAGAC AATTATATCC GTTCCTGTAA ATTGCTACCC
GATGGCTGCA CTCTCATAGT GGGAGGGGAA GCCAGTACTT TGTCCATTTG GGACCTGGCG
GCTCCAACCC CGCGCATCAA GGCGGAGCTG ACGTCCTCGG CCCCCGCCTG CTACGCCCTG
GCCATCAGCC CCGATTCCAA GGTCTGCTTC TCATGCTGCA GCGACGGCAA CATCGCTGTG
TGGGATCTGC ACAACCAGAC ACTAGTGAGG CAATTCCAGG GCCACACAGA CGGAGCCAGC
TGTATTGACA TTTCTAATGA TGGCACCAAG CTCTGGACGG GTGGTTTGGA CAACACAGTC
AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG CTGCAGCAGC ACGACTTCAC CTCCCAGATC
TTCTCCCTGG GGTACTGCCC CACCGGGGAG TGGCTGGCAG TGGGCATGGA GAGCAGCAAT
GTGGAGGTGC TGCACGTGAA CAAGCCTGAC AAGTACCAGC TGCACCTGCA TGAGAGCTGC
GTGCTGTCCC TGAAATTTGC TTACTGTGGT AAATGGTTTG TGAGTACTGG AAAAGATAAC
CTCCTCAATG CTTGGCGGAC CCCCTATGGA GCCAGCATAT TCCAGTCCAA AGAGTCCTCG
TCAGTGCTTA GCTGTGACAT CTCTGTGGAT GATAAGTACA TAGTCACTGG CTCGGGGGAC
AAGAAGGCTA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005068.2
CDS1296..3608
Misc Feature(1)1212..1214
Misc Feature(2)1296..1694
Misc Feature(3)1296..1688
Misc Feature(4)1689..1892
Misc Feature(5)1893..2099
Misc Feature(6)1968..1979
Misc Feature(7)2010..2012
Misc Feature(8)2070..2072
Misc Feature(9)2082..2084
Misc Feature(10)2094..2096
Misc Feature(11)2100..2645
Misc Feature(12)2151..2153
Misc Feature(13)2703..2798
Misc Feature(14)<2721..3599
Misc Feature(15)2742..3596
Misc Feature(16)order(2742..2744,2796..2798,2823..2828,2874..2879,2934..2936,2946..2948,2964..2969,3009..3014,3063..3065,3078..3080,3096..3101,3138..3140,3189..3191,3204..3206,3222..3227,3252..3257,3315..3317,3327..3329,3345..3350,3381..3386,3435..3437,3450..3452,3468..3473,3504..3509,3561..3563,3573..3575,3591..3596)
Misc Feature(17)2754..2873
Misc Feature(18)2877..2969
Misc Feature(19)2895..3011
Misc Feature(20)3009..3101
Misc Feature(21)3024..3134
Misc Feature(22)3135..3227
Misc Feature(23)3153..3260
Misc Feature(24)3273..3374
Misc Feature(25)3381..3473
Misc Feature(26)3396..3500
Misc Feature(27)3504..3596
Misc Feature(28)3519..3593
Exon (1)1..1319
Exon (2)1320..1420
Exon (3)1421..1484
Exon (4)1485..1529
Exon (5)1530..1592
Exon (6)1593..1667
Exon (7)1668..1872
Exon (8)1873..1889
Exon (9)1890..2006
Exon (10)2007..2060
Exon (11)2061..2213
Exon (12)2214..2358
Exon (13)2359..2549
Exon (14)2550..2626
Exon (15)2627..2876
Exon (16)2877..3124
Exon (17)3125..3272
Exon (18)3273..3423
Exon (19)3424..3500
Exon (20)3501..4123
Translation

Target ORF information:

RefSeq Version NM_005077.4
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005077.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT
CTCGGCAAGC CTCCAGCCAT AGACCCCCTC GTTAACCAAG CGGCAGCTGG CTTGAGGACA
CCCCTGGCAG TGCCCGGCCC ATATCCTGCT CCTTTTGGGA TGGTCCCCCA CGCTGGCATG
AACGGCGAGC TGACCAGCCC AGGCGCTGCC TACGCCAGTT TACACAACAT GTCGCCCCAG
ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG GTGGCCTACG GGCGCTCCCC CATGGTGGGG
TTTGATCCTC CCCCTCACAT GAGAGTACCT ACCATTCCTC CAAACCTGGC AGGAATCCCT
GGGGGGAAAC CTGCATACTC CTTCCACGTT ACTGCAGACG GTCAGATGCA GCCTGTCCCT
TTTCCCCCCG ACGCCCTCAT CGGACCCGGA ATCCCCCGGC ATGCTCGCCA GATCAACACC
CTCAACCACG GGGAGGTGGT GTGCGCTGTG ACCATCAGCA ACCCCACGAG ACACGTGTAC
ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG GACATCAGCC ACCCTGGCAA TAAGAGCCCT
GTCTCCCAGC TCGACTGTCT GAACAGAGAC AATTATATCC GTTCCTGTAA ATTGCTACCC
GATGGCTGCA CTCTCATAGT GGGAGGGGAA GCCAGTACTT TGTCCATTTG GGACCTGGCG
GCTCCAACCC CGCGCATCAA GGCGGAGCTG ACGTCCTCGG CCCCCGCCTG CTACGCCCTG
GCCATCAGCC CCGATTCCAA GGTCTGCTTC TCATGCTGCA GCGACGGCAA CATCGCTGTG
TGGGATCTGC ACAACCAGAC ACTAGTGAGG CAATTCCAGG GCCACACAGA CGGAGCCAGC
TGTATTGACA TTTCTAATGA TGGCACCAAG CTCTGGACGG GTGGTTTGGA CAACACAGTC
AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG CTGCAGCAGC ACGACTTCAC CTCCCAGATC
TTCTCCCTGG GGTACTGCCC CACCGGGGAG TGGCTGGCAG TGGGCATGGA GAGCAGCAAT
GTGGAGGTGC TGCACGTGAA CAAGCCTGAC AAGTACCAGC TGCACCTGCA TGAGAGCTGC
GTGCTGTCCC TGAAATTTGC TTACTGTGGT AAATGGTTTG TGAGTACTGG AAAAGATAAC
CTCCTCAATG CTTGGCGGAC CCCCTATGGA GCCAGCATAT TCCAGTCCAA AGAGTCCTCG
TCAGTGCTTA GCTGTGACAT CTCTGTGGAT GATAAGTACA TAGTCACTGG CTCGGGGGAC
AAGAAGGCTA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61007
Clone ID Related Accession (Same CDS sequence) NM_001303104.1
Accession Version NM_001303104.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2268bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB209854.1, AK293606.1, DB211613.1, AL353682.11 and BC010100.2. Transcript Variant: This variant (3) uses two alternate in-frame splice sites compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293606.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGCA
GCTGGCTTGA GGACACCCCT GGCAGTGCCC GGCCCATATC CTGCTCCTTT TGGGATGGTC
CCCCACGCTG GCATGAACGG CGAGCTGACC AGCCCAGGCG CTGCCTACGC CAGTTTACAC
AACATGTCGC CCCAGATGAG CGCCGCAGCC GCCGCGGCCG CCGTGGTGGC CTACGGGCGC
TCCCCCATGG TGGGGTTTGA TCCTCCCCCT CACATGAGAG TACCTACCAT TCCTCCAAAC
CTGGCAGGAA TCCCTGGGGG GAAACCTGCA TACTCCTTCC ACGTTACTGC AGACGGTCAG
ATGCAGCCTG TCCCTTTTCC CCCCGACGCC CTCATCGGAC CCGGAATCCC CCGGCATGCT
CGCCAGATCA ACACCCTCAA CCACGGGGAG GTGGTGTGCG CTGTGACCAT CAGCAACCCC
ACGAGACACG TGTACACAGG CGGGAAGGGC TGCGTCAAGG TCTGGGACAT CAGCCACCCT
GGCAATAAGA GCCCTGTCTC CCAGCTCGAC TGTCTGAACA GAGACAATTA TATCCGTTCC
TGTAAATTGC TACCCGATGG CTGCACTCTC ATAGTGGGAG GGGAAGCCAG TACTTTGTCC
ATTTGGGACC TGGCGGCTCC AACCCCGCGC ATCAAGGCGG AGCTGACGTC CTCGGCCCCC
GCCTGCTACG CCCTGGCCAT CAGCCCCGAT TCCAAGGTCT GCTTCTCATG CTGCAGCGAC
GGCAACATCG CTGTGTGGGA TCTGCACAAC CAGACACTAG TGAGGCAATT CCAGGGCCAC
ACAGACGGAG CCAGCTGTAT TGACATTTCT AATGATGGCA CCAAGCTCTG GACGGGTGGT
TTGGACAACA CAGTCAGGTC CTGGGACCTG CGCGAGGGGC GGCAGCTGCA GCAGCACGAC
TTCACCTCCC AGATCTTCTC CCTGGGGTAC TGCCCCACCG GGGAGTGGCT GGCAGTGGGC
ATGGAGAGCA GCAATGTGGA GGTGCTGCAC GTGAACAAGC CTGACAAGTA CCAGCTGCAC
CTGCATGAGA GCTGCGTGCT GTCCCTGAAA TTTGCTTACT GTGGTAAATG GTTTGTGAGT
ACTGGAAAAG ATAACCTCCT CAATGCTTGG CGGACCCCCT ATGGAGCCAG CATATTCCAG
TCCAAAGAGT CCTCGTCAGT GCTTAGCTGT GACATCTCTG TGGATGATAA GTACATAGTC
ACTGGCTCGG GGGACAAGAA GGCTACAGTC TATGAAGTCA TCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001290033.1
CDS1296..3563
Misc Feature(1)1212..1214
Misc Feature(2)1296..1694
Misc Feature(3)1296..1688
Misc Feature(4)1689..1892
Misc Feature(5)1893..2099
Misc Feature(6)1968..1979
Misc Feature(7)2010..2012
Misc Feature(8)2070..2072
Misc Feature(9)2082..2084
Misc Feature(10)2094..2096
Misc Feature(11)2151..2153
Misc Feature(12)2658..2753
Misc Feature(13)<2676..3554
Misc Feature(14)2697..3551
Misc Feature(15)order(2697..2699,2751..2753,2778..2783,2829..2834,2889..2891,2901..2903,2919..2924,2964..2969,3018..3020,3033..3035,3051..3056,3093..3095,3144..3146,3159..3161,3177..3182,3207..3212,3270..3272,3282..3284,3300..3305,3336..3341,3390..3392,3405..3407,3423..3428,3459..3464,3516..3518,3528..3530,3546..3551)
Misc Feature(16)2709..2828
Misc Feature(17)2832..2924
Misc Feature(18)2850..2966
Misc Feature(19)2964..3056
Misc Feature(20)2979..3089
Misc Feature(21)3090..3182
Misc Feature(22)3108..3215
Misc Feature(23)3228..3329
Misc Feature(24)3336..3428
Misc Feature(25)3351..3455
Misc Feature(26)3459..3551
Misc Feature(27)3474..3548
Exon (1)1..1319
Exon (2)1320..1420
Exon (3)1421..1484
Exon (4)1485..1529
Exon (5)1530..1592
Exon (6)1593..1667
Exon (7)1668..1872
Exon (8)1873..1889
Exon (9)1890..2006
Exon (10)2007..2060
Exon (11)2061..2213
Exon (12)2214..2313
Exon (13)2314..2504
Exon (14)2505..2581
Exon (15)2582..2831
Exon (16)2832..3079
Exon (17)3080..3227
Exon (18)3228..3378
Exon (19)3379..3455
Exon (20)3456..4078
Translation

Target ORF information:

RefSeq Version NM_001303104.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001303104.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGCA
GCTGGCTTGA GGACACCCCT GGCAGTGCCC GGCCCATATC CTGCTCCTTT TGGGATGGTC
CCCCACGCTG GCATGAACGG CGAGCTGACC AGCCCAGGCG CTGCCTACGC CAGTTTACAC
AACATGTCGC CCCAGATGAG CGCCGCAGCC GCCGCGGCCG CCGTGGTGGC CTACGGGCGC
TCCCCCATGG TGGGGTTTGA TCCTCCCCCT CACATGAGAG TACCTACCAT TCCTCCAAAC
CTGGCAGGAA TCCCTGGGGG GAAACCTGCA TACTCCTTCC ACGTTACTGC AGACGGTCAG
ATGCAGCCTG TCCCTTTTCC CCCCGACGCC CTCATCGGAC CCGGAATCCC CCGGCATGCT
CGCCAGATCA ACACCCTCAA CCACGGGGAG GTGGTGTGCG CTGTGACCAT CAGCAACCCC
ACGAGACACG TGTACACAGG CGGGAAGGGC TGCGTCAAGG TCTGGGACAT CAGCCACCCT
GGCAATAAGA GCCCTGTCTC CCAGCTCGAC TGTCTGAACA GAGACAATTA TATCCGTTCC
TGTAAATTGC TACCCGATGG CTGCACTCTC ATAGTGGGAG GGGAAGCCAG TACTTTGTCC
ATTTGGGACC TGGCGGCTCC AACCCCGCGC ATCAAGGCGG AGCTGACGTC CTCGGCCCCC
GCCTGCTACG CCCTGGCCAT CAGCCCCGAT TCCAAGGTCT GCTTCTCATG CTGCAGCGAC
GGCAACATCG CTGTGTGGGA TCTGCACAAC CAGACACTAG TGAGGCAATT CCAGGGCCAC
ACAGACGGAG CCAGCTGTAT TGACATTTCT AATGATGGCA CCAAGCTCTG GACGGGTGGT
TTGGACAACA CAGTCAGGTC CTGGGACCTG CGCGAGGGGC GGCAGCTGCA GCAGCACGAC
TTCACCTCCC AGATCTTCTC CCTGGGGTAC TGCCCCACCG GGGAGTGGCT GGCAGTGGGC
ATGGAGAGCA GCAATGTGGA GGTGCTGCAC GTGAACAAGC CTGACAAGTA CCAGCTGCAC
CTGCATGAGA GCTGCGTGCT GTCCCTGAAA TTTGCTTACT GTGGTAAATG GTTTGTGAGT
ACTGGAAAAG ATAACCTCCT CAATGCTTGG CGGACCCCCT ATGGAGCCAG CATATTCCAG
TCCAAAGAGT CCTCGTCAGT GCTTAGCTGT GACATCTCTG TGGATGATAA GTACATAGTC
ACTGGCTCGG GGGACAAGAA GGCTACAGTC TATGAAGTCA TCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43466
Clone ID Related Accession (Same CDS sequence) XM_005252151.1
Accession Version XM_005252151.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2445bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGGATTGGA TTTTCATTTG GAATTGGTAC CAGGGAATGG AAAGTTCGGA
CTGAAAGAGA AATCTTGGAT TTTATCGAGA AAATCTTCTT TTGAGATTAA GTCAGCAGCT
GGCTTGAGGA CACCCCTGGC AGTGCCCGGC CCATATCCTG CTCCTTTTGG GATGGTCCCC
CACGCTGGCA TGAACGGCGA GCTGACCAGC CCAGGCGCTG CCTACGCCAG TTTACACAAC
ATGTCGCCCC AGATGAGCGC CGCAGCCGCC GCGGCCGCCG TGGTGGCCTA CGGGCGCTCC
CCCATGGTGG GGTTTGATCC TCCCCCTCAC ATGAGAGTAC CTACCATTCC TCCAAACCTG
GCAGGAATCC CTGGGGGGAA ACCTGCATAC TCCTTCCACG TTACTGCAGA CGGTCAGATG
CAGCCTGTCC CTTTTCCCCC CGACGCCCTC ATCGGACCCG GAATCCCCCG GCATGCTCGC
CAGATCAACA CCCTCAACCA CGGGGAGGTG GTGTGCGCTG TGACCATCAG CAACCCCACG
AGACACGTGT ACACAGGCGG GAAGGGCTGC GTCAAGGTCT GGGACATCAG CCACCCTGGC
AATAAGAGCC CTGTCTCCCA GCTCGACTGT CTGAACAGAG ACAATTATAT CCGTTCCTGT
AAATTGCTAC CCGATGGCTG CACTCTCATA GTGGGAGGGG AAGCCAGTAC TTTGTCCATT
TGGGACCTGG CGGCTCCAAC CCCGCGCATC AAGGCGGAGC TGACGTCCTC GGCCCCCGCC
TGCTACGCCC TGGCCATCAG CCCCGATTCC AAGGTCTGCT TCTCATGCTG CAGCGACGGC
AACATCGCTG TGTGGGATCT GCACAACCAG ACACTAGTGA GGCAATTCCA GGGCCACACA
GACGGAGCCA GCTGTATTGA CATTTCTAAT GATGGCACCA AGCTCTGGAC GGGTGGTTTG
GACAACACAG TCAGGTCCTG GGACCTGCGC GAGGGGCGGC AGCTGCAGCA GCACGACTTC
ACCTCCCAGA TCTTCTCCCT GGGGTACTGC CCCACCGGGG AGTGGCTGGC AGTGGGCATG
GAGAGCAGCA ATGTGGAGGT GCTGCACGTG AACAAGCCTG ACAAGTACCA GCTGCACCTG
CATGAGAGCT GCGTGCTGTC CCTGAAATTT GCTTACTGTG GTAAATGGTT TGTGAGTACT
GGAAAAGATA ACCTCCTCAA TGCTTGGCGG ACCCCCTATG GAGCCAGCAT ATTCCAGTCC
AAAGAGTCCT CGTCAGTGCT TAGCTGTGAC ATCTCTGTGG ATGATAAGTA CATAGTCACT
GGCTCGGGGG ACAAGAAGGC TACAGTCTAT GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252208.1
CDS210..2654
Misc Feature(1)210..638
Misc Feature(2)<1767..2645
Misc Feature(3)1788..2642
Misc Feature(4)order(1788..1790,1842..1844,1869..1874,1920..1925,1980..1982,1992..1994,2010..2015,2055..2060,2109..2111,2124..2126,2142..2147,2184..2186,2235..2237,2250..2252,2268..2273,2298..2303,2361..2363,2373..2375,2391..2396,2427..2432,2481..2483,2496..2498,2514..2519,2550..2555,2607..2609,2619..2621,2637..2642)
Misc Feature(5)1800..1919
Misc Feature(6)1941..2057
Misc Feature(7)2070..2180
Misc Feature(8)2199..2306
Misc Feature(9)2319..2420
Misc Feature(10)2442..2546
Misc Feature(11)2565..2639
Translation

Target ORF information:

RefSeq Version XM_005252151.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252151.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGGATTGGA TTTTCATTTG GAATTGGTAC CAGGGAATGG AAAGTTCGGA
CTGAAAGAGA AATCTTGGAT TTTATCGAGA AAATCTTCTT TTGAGATTAA GTCAGCAGCT
GGCTTGAGGA CACCCCTGGC AGTGCCCGGC CCATATCCTG CTCCTTTTGG GATGGTCCCC
CACGCTGGCA TGAACGGCGA GCTGACCAGC CCAGGCGCTG CCTACGCCAG TTTACACAAC
ATGTCGCCCC AGATGAGCGC CGCAGCCGCC GCGGCCGCCG TGGTGGCCTA CGGGCGCTCC
CCCATGGTGG GGTTTGATCC TCCCCCTCAC ATGAGAGTAC CTACCATTCC TCCAAACCTG
GCAGGAATCC CTGGGGGGAA ACCTGCATAC TCCTTCCACG TTACTGCAGA CGGTCAGATG
CAGCCTGTCC CTTTTCCCCC CGACGCCCTC ATCGGACCCG GAATCCCCCG GCATGCTCGC
CAGATCAACA CCCTCAACCA CGGGGAGGTG GTGTGCGCTG TGACCATCAG CAACCCCACG
AGACACGTGT ACACAGGCGG GAAGGGCTGC GTCAAGGTCT GGGACATCAG CCACCCTGGC
AATAAGAGCC CTGTCTCCCA GCTCGACTGT CTGAACAGAG ACAATTATAT CCGTTCCTGT
AAATTGCTAC CCGATGGCTG CACTCTCATA GTGGGAGGGG AAGCCAGTAC TTTGTCCATT
TGGGACCTGG CGGCTCCAAC CCCGCGCATC AAGGCGGAGC TGACGTCCTC GGCCCCCGCC
TGCTACGCCC TGGCCATCAG CCCCGATTCC AAGGTCTGCT TCTCATGCTG CAGCGACGGC
AACATCGCTG TGTGGGATCT GCACAACCAG ACACTAGTGA GGCAATTCCA GGGCCACACA
GACGGAGCCA GCTGTATTGA CATTTCTAAT GATGGCACCA AGCTCTGGAC GGGTGGTTTG
GACAACACAG TCAGGTCCTG GGACCTGCGC GAGGGGCGGC AGCTGCAGCA GCACGACTTC
ACCTCCCAGA TCTTCTCCCT GGGGTACTGC CCCACCGGGG AGTGGCTGGC AGTGGGCATG
GAGAGCAGCA ATGTGGAGGT GCTGCACGTG AACAAGCCTG ACAAGTACCA GCTGCACCTG
CATGAGAGCT GCGTGCTGTC CCTGAAATTT GCTTACTGTG GTAAATGGTT TGTGAGTACT
GGAAAAGATA ACCTCCTCAA TGCTTGGCGG ACCCCCTATG GAGCCAGCAT ATTCCAGTCC
AAAGAGTCCT CGTCAGTGCT TAGCTGTGAC ATCTCTGTGG ATGATAAGTA CATAGTCACT
GGCTCGGGGG ACAAGAAGGC TACAGTCTAT GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43467
Clone ID Related Accession (Same CDS sequence) XM_005252152.1
Accession Version XM_005252152.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2442bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGGATTGGA TTTTCATTTG GAATTGGTAC CAGGGAATGG AAAGTTCGGA
CTGAAAGAGA AATCTTGGAT TTTATCGAGA AAATCTTCTT TTGAGATTAA GTCAGCTGGC
TTGAGGACAC CCCTGGCAGT GCCCGGCCCA TATCCTGCTC CTTTTGGGAT GGTCCCCCAC
GCTGGCATGA ACGGCGAGCT GACCAGCCCA GGCGCTGCCT ACGCCAGTTT ACACAACATG
TCGCCCCAGA TGAGCGCCGC AGCCGCCGCG GCCGCCGTGG TGGCCTACGG GCGCTCCCCC
ATGGTGGGGT TTGATCCTCC CCCTCACATG AGAGTACCTA CCATTCCTCC AAACCTGGCA
GGAATCCCTG GGGGGAAACC TGCATACTCC TTCCACGTTA CTGCAGACGG TCAGATGCAG
CCTGTCCCTT TTCCCCCCGA CGCCCTCATC GGACCCGGAA TCCCCCGGCA TGCTCGCCAG
ATCAACACCC TCAACCACGG GGAGGTGGTG TGCGCTGTGA CCATCAGCAA CCCCACGAGA
CACGTGTACA CAGGCGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAT
AAGAGCCCTG TCTCCCAGCT CGACTGTCTG AACAGAGACA ATTATATCCG TTCCTGTAAA
TTGCTACCCG ATGGCTGCAC TCTCATAGTG GGAGGGGAAG CCAGTACTTT GTCCATTTGG
GACCTGGCGG CTCCAACCCC GCGCATCAAG GCGGAGCTGA CGTCCTCGGC CCCCGCCTGC
TACGCCCTGG CCATCAGCCC CGATTCCAAG GTCTGCTTCT CATGCTGCAG CGACGGCAAC
ATCGCTGTGT GGGATCTGCA CAACCAGACA CTAGTGAGGC AATTCCAGGG CCACACAGAC
GGAGCCAGCT GTATTGACAT TTCTAATGAT GGCACCAAGC TCTGGACGGG TGGTTTGGAC
AACACAGTCA GGTCCTGGGA CCTGCGCGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
TCCCAGATCT TCTCCCTGGG GTACTGCCCC ACCGGGGAGT GGCTGGCAGT GGGCATGGAG
AGCAGCAATG TGGAGGTGCT GCACGTGAAC AAGCCTGACA AGTACCAGCT GCACCTGCAT
GAGAGCTGCG TGCTGTCCCT GAAATTTGCT TACTGTGGTA AATGGTTTGT GAGTACTGGA
AAAGATAACC TCCTCAATGC TTGGCGGACC CCCTATGGAG CCAGCATATT CCAGTCCAAA
GAGTCCTCGT CAGTGCTTAG CTGTGACATC TCTGTGGATG ATAAGTACAT AGTCACTGGC
TCGGGGGACA AGAAGGCTAC AGTCTATGAA GTCATCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252209.1
CDS210..2651
Misc Feature(1)210..638
Misc Feature(2)<1764..2642
Misc Feature(3)1785..2639
Misc Feature(4)order(1785..1787,1839..1841,1866..1871,1917..1922,1977..1979,1989..1991,2007..2012,2052..2057,2106..2108,2121..2123,2139..2144,2181..2183,2232..2234,2247..2249,2265..2270,2295..2300,2358..2360,2370..2372,2388..2393,2424..2429,2478..2480,2493..2495,2511..2516,2547..2552,2604..2606,2616..2618,2634..2639)
Misc Feature(5)1797..1916
Misc Feature(6)1938..2054
Misc Feature(7)2067..2177
Misc Feature(8)2196..2303
Misc Feature(9)2316..2417
Misc Feature(10)2439..2543
Misc Feature(11)2562..2636
Translation

Target ORF information:

RefSeq Version XM_005252152.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252152.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGGATTGGA TTTTCATTTG GAATTGGTAC CAGGGAATGG AAAGTTCGGA
CTGAAAGAGA AATCTTGGAT TTTATCGAGA AAATCTTCTT TTGAGATTAA GTCAGCTGGC
TTGAGGACAC CCCTGGCAGT GCCCGGCCCA TATCCTGCTC CTTTTGGGAT GGTCCCCCAC
GCTGGCATGA ACGGCGAGCT GACCAGCCCA GGCGCTGCCT ACGCCAGTTT ACACAACATG
TCGCCCCAGA TGAGCGCCGC AGCCGCCGCG GCCGCCGTGG TGGCCTACGG GCGCTCCCCC
ATGGTGGGGT TTGATCCTCC CCCTCACATG AGAGTACCTA CCATTCCTCC AAACCTGGCA
GGAATCCCTG GGGGGAAACC TGCATACTCC TTCCACGTTA CTGCAGACGG TCAGATGCAG
CCTGTCCCTT TTCCCCCCGA CGCCCTCATC GGACCCGGAA TCCCCCGGCA TGCTCGCCAG
ATCAACACCC TCAACCACGG GGAGGTGGTG TGCGCTGTGA CCATCAGCAA CCCCACGAGA
CACGTGTACA CAGGCGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAT
AAGAGCCCTG TCTCCCAGCT CGACTGTCTG AACAGAGACA ATTATATCCG TTCCTGTAAA
TTGCTACCCG ATGGCTGCAC TCTCATAGTG GGAGGGGAAG CCAGTACTTT GTCCATTTGG
GACCTGGCGG CTCCAACCCC GCGCATCAAG GCGGAGCTGA CGTCCTCGGC CCCCGCCTGC
TACGCCCTGG CCATCAGCCC CGATTCCAAG GTCTGCTTCT CATGCTGCAG CGACGGCAAC
ATCGCTGTGT GGGATCTGCA CAACCAGACA CTAGTGAGGC AATTCCAGGG CCACACAGAC
GGAGCCAGCT GTATTGACAT TTCTAATGAT GGCACCAAGC TCTGGACGGG TGGTTTGGAC
AACACAGTCA GGTCCTGGGA CCTGCGCGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
TCCCAGATCT TCTCCCTGGG GTACTGCCCC ACCGGGGAGT GGCTGGCAGT GGGCATGGAG
AGCAGCAATG TGGAGGTGCT GCACGTGAAC AAGCCTGACA AGTACCAGCT GCACCTGCAT
GAGAGCTGCG TGCTGTCCCT GAAATTTGCT TACTGTGGTA AATGGTTTGT GAGTACTGGA
AAAGATAACC TCCTCAATGC TTGGCGGACC CCCTATGGAG CCAGCATATT CCAGTCCAAA
GAGTCCTCGT CAGTGCTTAG CTGTGACATC TCTGTGGATG ATAAGTACAT AGTCACTGGC
TCGGGGGACA AGAAGGCTAC AGTCTATGAA GTCATCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43468
Clone ID Related Accession (Same CDS sequence) XM_006717258.1
Accession Version XM_006717258.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2442bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGTT GCAAGCTCAG
CATCTTTCTC ATGGCCACGG ACCCCCAGTT CCCCTTACGC CTCACCCTTC GGGACTTCAG
CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG
AGTGGGCAGT CTCACTTGGC AATAAAAGAT GACAAGAAGC ACCACGATGC AGAGCACCAC
AGAGACAGAG AGCCGGGCAC AAGTAATTCC CTCCTGGTCC CAGACAGTCT AAGAGGCACA
GATAAACGCA GAAATGGACC TGAATTTTCC AATGACATCA AGAAAAGGAA GGTGGATGAT
AAGGACTCCA GCCACTATGA CAGTGATGGT GACAAAAGCG ATGACAACTT AGTTGTGGAT
GTGTCTAATG AGGACCCTTC TTCTCCGCGA GCAAGCCCTG CCCACTCGCC CCGGGAAAAT
GGAATCGACA AAAATCGCCT GCTAAAGAAG GATGCTTCTA GCAGTCCAGC TTCCACGGCC
TCCTCGGCAA GTTCCACTTC TTTGAAATCC AAAGAAATGA GCTTGCATGA AAAAGCCAGC
ACGCCTGTTC TGAAATCCAG CACACCAACG CCTCGGAGCG ACATGCCAAC GCCGGGCACC
AGCGCCACTC CAGGCCTCCG TCCAGGTCTC GGCAAGCCTC CAGCCATAGA CCCCCTCGTT
AACCAAGCGG GATTGGATTT TCATTTGGAA TTGGTACCAG GGAATGGAAA GTTCGGACTG
AAAGAGAAAT CTTGGATTTT ATCGAGAAAA TCTTCTTTTG AGATTAAGTC AGCAGCTGGC
TTGAGGACAC CCCTGGCAGT GCCCGGCCCA TATCCTGCTC CTTTTGGGAT GGTCCCCCAC
GCTGGCATGA ACGGCGAGCT GACCAGCCCA GGCGCTGCCT ACGCCAGTTT ACACAACATG
TCGCCCCAGA TGAGCGCCGC AGCCGCCGCG GCCGCCGTGG TGGCCTACGG GCGCTCCCCC
ATGGTGGGGT TTGATCCTCC CCCTCACATG AGAGTACCTA CCATTCCTCC AAACCTGGCA
GGAATCCCTG GGGGGAAACC TGCATACTCC TTCCACGTTA CTGCAGACGG TCAGATGCAG
CCTGTCCCTT TTCCCCCCGA CGCCCTCATC GGACCCGGAA TCCCCCGGCA TGCTCGCCAG
ATCAACACCC TCAACCACGG GGAGGTGGTG TGCGCTGTGA CCATCAGCAA CCCCACGAGA
CACGTGTACA CAGGCGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAT
AAGAGCCCTG TCTCCCAGCT CGACTGTCTG AACAGAGACA ATTATATCCG TTCCTGTAAA
TTGCTACCCG ATGGCTGCAC TCTCATAGTG GGAGGGGAAG CCAGTACTTT GTCCATTTGG
GACCTGGCGG CTCCAACCCC GCGCATCAAG GCGGAGCTGA CGTCCTCGGC CCCCGCCTGC
TACGCCCTGG CCATCAGCCC CGATTCCAAG GTCTGCTTCT CATGCTGCAG CGACGGCAAC
ATCGCTGTGT GGGATCTGCA CAACCAGACA CTAGTGAGGC AATTCCAGGG CCACACAGAC
GGAGCCAGCT GTATTGACAT TTCTAATGAT GGCACCAAGC TCTGGACGGG TGGTTTGGAC
AACACAGTCA GGTCCTGGGA CCTGCGCGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
TCCCAGATCT TCTCCCTGGG GTACTGCCCC ACCGGGGAGT GGCTGGCAGT GGGCATGGAG
AGCAGCAATG TGGAGGTGCT GCACGTGAAC AAGCCTGACA AGTACCAGCT GCACCTGCAT
GAGAGCTGCG TGCTGTCCCT GAAATTTGCT TACTGTGGTA AATGGTTTGT GAGTACTGGA
AAAGATAACC TCCTCAATGC TTGGCGGACC CCCTATGGAG CCAGCATATT CCAGTCCAAA
GAGTCCTCGT CAGTGCTTAG CTGTGACATC TCTGTGGATG ATAAGTACAT AGTCACTGGC
TCGGGGGACA AGAAGGCTAC AGTCTATGAA GTCATCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717321.1
CDS210..2651
Misc Feature(1)210..635
Misc Feature(2)<1764..2642
Misc Feature(3)1785..2639
Misc Feature(4)order(1785..1787,1839..1841,1866..1871,1917..1922,1977..1979,1989..1991,2007..2012,2052..2057,2106..2108,2121..2123,2139..2144,2181..2183,2232..2234,2247..2249,2265..2270,2295..2300,2358..2360,2370..2372,2388..2393,2424..2429,2478..2480,2493..2495,2511..2516,2547..2552,2604..2606,2616..2618,2634..2639)
Misc Feature(5)1797..1916
Misc Feature(6)1938..2054
Misc Feature(7)2067..2177
Misc Feature(8)2196..2303
Misc Feature(9)2316..2417
Misc Feature(10)2439..2543
Misc Feature(11)2562..2636
Translation

Target ORF information:

RefSeq Version XM_006717258.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717258.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGTT GCAAGCTCAG
CATCTTTCTC ATGGCCACGG ACCCCCAGTT CCCCTTACGC CTCACCCTTC GGGACTTCAG
CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG
AGTGGGCAGT CTCACTTGGC AATAAAAGAT GACAAGAAGC ACCACGATGC AGAGCACCAC
AGAGACAGAG AGCCGGGCAC AAGTAATTCC CTCCTGGTCC CAGACAGTCT AAGAGGCACA
GATAAACGCA GAAATGGACC TGAATTTTCC AATGACATCA AGAAAAGGAA GGTGGATGAT
AAGGACTCCA GCCACTATGA CAGTGATGGT GACAAAAGCG ATGACAACTT AGTTGTGGAT
GTGTCTAATG AGGACCCTTC TTCTCCGCGA GCAAGCCCTG CCCACTCGCC CCGGGAAAAT
GGAATCGACA AAAATCGCCT GCTAAAGAAG GATGCTTCTA GCAGTCCAGC TTCCACGGCC
TCCTCGGCAA GTTCCACTTC TTTGAAATCC AAAGAAATGA GCTTGCATGA AAAAGCCAGC
ACGCCTGTTC TGAAATCCAG CACACCAACG CCTCGGAGCG ACATGCCAAC GCCGGGCACC
AGCGCCACTC CAGGCCTCCG TCCAGGTCTC GGCAAGCCTC CAGCCATAGA CCCCCTCGTT
AACCAAGCGG GATTGGATTT TCATTTGGAA TTGGTACCAG GGAATGGAAA GTTCGGACTG
AAAGAGAAAT CTTGGATTTT ATCGAGAAAA TCTTCTTTTG AGATTAAGTC AGCAGCTGGC
TTGAGGACAC CCCTGGCAGT GCCCGGCCCA TATCCTGCTC CTTTTGGGAT GGTCCCCCAC
GCTGGCATGA ACGGCGAGCT GACCAGCCCA GGCGCTGCCT ACGCCAGTTT ACACAACATG
TCGCCCCAGA TGAGCGCCGC AGCCGCCGCG GCCGCCGTGG TGGCCTACGG GCGCTCCCCC
ATGGTGGGGT TTGATCCTCC CCCTCACATG AGAGTACCTA CCATTCCTCC AAACCTGGCA
GGAATCCCTG GGGGGAAACC TGCATACTCC TTCCACGTTA CTGCAGACGG TCAGATGCAG
CCTGTCCCTT TTCCCCCCGA CGCCCTCATC GGACCCGGAA TCCCCCGGCA TGCTCGCCAG
ATCAACACCC TCAACCACGG GGAGGTGGTG TGCGCTGTGA CCATCAGCAA CCCCACGAGA
CACGTGTACA CAGGCGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAT
AAGAGCCCTG TCTCCCAGCT CGACTGTCTG AACAGAGACA ATTATATCCG TTCCTGTAAA
TTGCTACCCG ATGGCTGCAC TCTCATAGTG GGAGGGGAAG CCAGTACTTT GTCCATTTGG
GACCTGGCGG CTCCAACCCC GCGCATCAAG GCGGAGCTGA CGTCCTCGGC CCCCGCCTGC
TACGCCCTGG CCATCAGCCC CGATTCCAAG GTCTGCTTCT CATGCTGCAG CGACGGCAAC
ATCGCTGTGT GGGATCTGCA CAACCAGACA CTAGTGAGGC AATTCCAGGG CCACACAGAC
GGAGCCAGCT GTATTGACAT TTCTAATGAT GGCACCAAGC TCTGGACGGG TGGTTTGGAC
AACACAGTCA GGTCCTGGGA CCTGCGCGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
TCCCAGATCT TCTCCCTGGG GTACTGCCCC ACCGGGGAGT GGCTGGCAGT GGGCATGGAG
AGCAGCAATG TGGAGGTGCT GCACGTGAAC AAGCCTGACA AGTACCAGCT GCACCTGCAT
GAGAGCTGCG TGCTGTCCCT GAAATTTGCT TACTGTGGTA AATGGTTTGT GAGTACTGGA
AAAGATAACC TCCTCAATGC TTGGCGGACC CCCTATGGAG CCAGCATATT CCAGTCCAAA
GAGTCCTCGT CAGTGCTTAG CTGTGACATC TCTGTGGATG ATAAGTACAT AGTCACTGGC
TCGGGGGACA AGAAGGCTAC AGTCTATGAA GTCATCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43469
Clone ID Related Accession (Same CDS sequence) XM_005252153.1
Accession Version XM_005252153.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT
CTCGGCAAGC CTCCAGCCAT AGACCCCCTC GTTAACCAAG CGGGATTGGA TTTTCATTTG
GAATTGGTAC CAGGGAATGG AAAGTTCGGA CTGAAAGAGA AATCTTGGAT TTTATCGAGA
AAATCTTCTT TTGAGATTAA GTCAGCAGCT GGCTTGAGGA CACCCCTGGC AGTGCCCGGC
CCATATCCTG CTCCTTTTGG GATGGTCCCC CACGCTGGCA TGAACGGCGA GCTGACCAGC
CCAGGCGCTG CCTACGCCAG TTTACACAAC ATGTCGCCCC AGATGAGCGC CGCAGCCGCC
GCGGCCGCCG TGGTGGCCTA CGGGCGCTCC CCCATGGTGG GGTTTGATCC TCCCCCTCAC
ATGAGAGTAC CTACCATTCC TCCAAACCTG GCAGGAATCC CTGGGGGGAA ACCTGCATAC
TCCTTCCACG TTACTGCAGA CGGTCAGATG CAGCCTGTCC CTTTTCCCCC CGACGCCCTC
ATCGGACCCG GAATCCCCCG GCATGCTCGC CAGATCAACA CCCTCAACCA CGGGGAGGTG
GTGTGCGCTG TGACCATCAG CAACCCCACG AGACACGTGT ACACAGGCGG GAAGGGCTGC
GTCAAGGTCT GGGACATCAG CCACCCTGGC AATAAGAGCC CTGTCTCCCA GCTCGACTGT
CTGAACAGAG ACAATTATAT CCGTTCCTGT AAATTGCTAC CCGATGGCTG CACTCTCATA
GTGGGAGGGG AAGCCAGTAC TTTGTCCATT TGGGACCTGG CGGCTCCAAC CCCGCGCATC
AAGGCGGAGC TGACGTCCTC GGCCCCCGCC TGCTACGCCC TGGCCATCAG CCCCGATTCC
AAGGTCTGCT TCTCATGCTG CAGCGACGGC AACATCGCTG TGTGGGATCT GCACAACCAG
ACACTAGTGA GGCAATTCCA GGGCCACACA GACGGAGCCA GCTGTATTGA CATTTCTAAT
GATGGCACCA AGCTCTGGAC GGGTGGTTTG GACAACACAG TCAGGTCCTG GGACCTGCGC
GAGGGGCGGC AGCTGCAGCA GCACGACTTC ACCTCCCAGA TCTTCTCCCT GGGGTACTGC
CCCACCGGGG AGTGGCTGGC AGTGGGCATG GAGAGCAGCA ATGTGGAGGT GCTGCACGTG
AACAAGCCTG ACAAGTACCA GCTGCACCTG CATGAGAGCT GCGTGCTGTC CCTGAAATTT
GCTTACTGTG GTAAATGGTT TGTGAGTACT GGAAAAGATA ACCTCCTCAA TGCTTGGCGG
ACCCCCTATG GAGCCAGCAT ATTCCAGTCC AAAGAGTCCT CGTCAGTGCT TAGCTGTGAC
ATCTCTGTGG ATGATAAGTA CATAGTCACT GGCTCGGGGG ACAAGAAGGC TACAGTCTAT
GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252210.1
CDS210..2624
Misc Feature(1)210..608
Misc Feature(2)<1737..2615
Misc Feature(3)1758..2612
Misc Feature(4)order(1758..1760,1812..1814,1839..1844,1890..1895,1950..1952,1962..1964,1980..1985,2025..2030,2079..2081,2094..2096,2112..2117,2154..2156,2205..2207,2220..2222,2238..2243,2268..2273,2331..2333,2343..2345,2361..2366,2397..2402,2451..2453,2466..2468,2484..2489,2520..2525,2577..2579,2589..2591,2607..2612)
Misc Feature(5)1770..1889
Misc Feature(6)1911..2027
Misc Feature(7)2040..2150
Misc Feature(8)2169..2276
Misc Feature(9)2289..2390
Misc Feature(10)2412..2516
Misc Feature(11)2535..2609
Translation

Target ORF information:

RefSeq Version XM_005252153.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252153.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT
CTCGGCAAGC CTCCAGCCAT AGACCCCCTC GTTAACCAAG CGGGATTGGA TTTTCATTTG
GAATTGGTAC CAGGGAATGG AAAGTTCGGA CTGAAAGAGA AATCTTGGAT TTTATCGAGA
AAATCTTCTT TTGAGATTAA GTCAGCAGCT GGCTTGAGGA CACCCCTGGC AGTGCCCGGC
CCATATCCTG CTCCTTTTGG GATGGTCCCC CACGCTGGCA TGAACGGCGA GCTGACCAGC
CCAGGCGCTG CCTACGCCAG TTTACACAAC ATGTCGCCCC AGATGAGCGC CGCAGCCGCC
GCGGCCGCCG TGGTGGCCTA CGGGCGCTCC CCCATGGTGG GGTTTGATCC TCCCCCTCAC
ATGAGAGTAC CTACCATTCC TCCAAACCTG GCAGGAATCC CTGGGGGGAA ACCTGCATAC
TCCTTCCACG TTACTGCAGA CGGTCAGATG CAGCCTGTCC CTTTTCCCCC CGACGCCCTC
ATCGGACCCG GAATCCCCCG GCATGCTCGC CAGATCAACA CCCTCAACCA CGGGGAGGTG
GTGTGCGCTG TGACCATCAG CAACCCCACG AGACACGTGT ACACAGGCGG GAAGGGCTGC
GTCAAGGTCT GGGACATCAG CCACCCTGGC AATAAGAGCC CTGTCTCCCA GCTCGACTGT
CTGAACAGAG ACAATTATAT CCGTTCCTGT AAATTGCTAC CCGATGGCTG CACTCTCATA
GTGGGAGGGG AAGCCAGTAC TTTGTCCATT TGGGACCTGG CGGCTCCAAC CCCGCGCATC
AAGGCGGAGC TGACGTCCTC GGCCCCCGCC TGCTACGCCC TGGCCATCAG CCCCGATTCC
AAGGTCTGCT TCTCATGCTG CAGCGACGGC AACATCGCTG TGTGGGATCT GCACAACCAG
ACACTAGTGA GGCAATTCCA GGGCCACACA GACGGAGCCA GCTGTATTGA CATTTCTAAT
GATGGCACCA AGCTCTGGAC GGGTGGTTTG GACAACACAG TCAGGTCCTG GGACCTGCGC
GAGGGGCGGC AGCTGCAGCA GCACGACTTC ACCTCCCAGA TCTTCTCCCT GGGGTACTGC
CCCACCGGGG AGTGGCTGGC AGTGGGCATG GAGAGCAGCA ATGTGGAGGT GCTGCACGTG
AACAAGCCTG ACAAGTACCA GCTGCACCTG CATGAGAGCT GCGTGCTGTC CCTGAAATTT
GCTTACTGTG GTAAATGGTT TGTGAGTACT GGAAAAGATA ACCTCCTCAA TGCTTGGCGG
ACCCCCTATG GAGCCAGCAT ATTCCAGTCC AAAGAGTCCT CGTCAGTGCT TAGCTGTGAC
ATCTCTGTGG ATGATAAGTA CATAGTCACT GGCTCGGGGG ACAAGAAGGC TACAGTCTAT
GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43470
Clone ID Related Accession (Same CDS sequence) XM_005252154.1
Accession Version XM_005252154.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGTT GCAAGCTCAG CATCTTTCTC ATGGCCACGG ACCCCCAGTT
CCCCTTACGC CTCACCCTTC GGGACTTCAG CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT
GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG AGTGGGCAGT CTCACTTGGC AATAAAAGAT
GACAAGAAGC ACCACGATGC AGAGCACCAC AGAGACAGAG AGCCGGGCAC AAGTAATTCC
CTCCTGGTCC CAGACAGTCT AAGAGGCACA GATAAACGCA GAAATGGACC TGAATTTTCC
AATGACATCA AGAAAAGGAA GGTGGATGAT AAGGACTCCA GCCACTATGA CAGTGATGGT
GACAAAAGCG ATGACAACTT AGTTGTGGAT GTGTCTAATG AGGACCCTTC TTCTCCGCGA
GCAAGCCCTG CCCACTCGCC CCGGGAAAAT GGAATCGACA AAAATCGCCT GCTAAAGAAG
GATGCTTCTA GCAGTCCAGC TTCCACGGCC TCCTCGGCAA GTTCCACTTC TTTGAAATCC
AAAGAAATGA GCTTGCATGA AAAAGCCAGC ACGCCTGTTC TGAAATCCAG CACACCAACG
CCTCGGAGCG ACATGCCAAC GCCGGGCACC AGCGCCACTC CAGGCCTCCG TCCAGGTCTC
GGCAAGCCTC CAGCCATAGA CCCCCTCGTT AACCAAGCGG GATTGGATTT TCATTTGGAA
TTGGTACCAG GGAATGGAAA GTTCGGACTG AAAGAGAAAT CTTGGATTTT ATCGAGAAAA
TCTTCTTTTG AGATTAAGTC AGCAGCTGGC TTGAGGACAC CCCTGGCAGT GCCCGGCCCA
TATCCTGCTC CTTTTGGGAT GGTCCCCCAC GCTGGCATGA ACGGCGAGCT GACCAGCCCA
GGCGCTGCCT ACGCCAGTTT ACACAACATG TCGCCCCAGA TGAGCGCCGC AGCCGCCGCG
GCCGCCGTGG TGGCCTACGG GCGCTCCCCC ATGGTGGGGT TTGATCCTCC CCCTCACATG
AGAGTACCTA CCATTCCTCC AAACCTGGCA GGAATCCCTG GGGGGAAACC TGCATACTCC
TTCCACGTTA CTGCAGACGG TCAGATGCAG CCTGTCCCTT TTCCCCCCGA CGCCCTCATC
GGACCCGGAA TCCCCCGGCA TGCTCGCCAG ATCAACACCC TCAACCACGG GGAGGTGGTG
TGCGCTGTGA CCATCAGCAA CCCCACGAGA CACGTGTACA CAGGCGGGAA GGGCTGCGTC
AAGGTCTGGG ACATCAGCCA CCCTGGCAAT AAGAGCCCTG TCTCCCAGCT CGACTGTCTG
AACAGAGACA ATTATATCCG TTCCTGTAAA TTGCTACCCG ATGGCTGCAC TCTCATAGTG
GGAGGGGAAG CCAGTACTTT GTCCATTTGG GACCTGGCGG CTCCAACCCC GCGCATCAAG
GCGGAGCTGA CGTCCTCGGC CCCCGCCTGC TACGCCCTGG CCATCAGCCC CGATTCCAAG
GTCTGCTTCT CATGCTGCAG CGACGGCAAC ATCGCTGTGT GGGATCTGCA CAACCAGACA
CTAGTGAGGC AATTCCAGGG CCACACAGAC GGAGCCAGCT GTATTGACAT TTCTAATGAT
GGCACCAAGC TCTGGACGGG TGGTTTGGAC AACACAGTCA GGTCCTGGGA CCTGCGCGAG
GGGCGGCAGC TGCAGCAGCA CGACTTCACC TCCCAGATCT TCTCCCTGGG GTACTGCCCC
ACCGGGGAGT GGCTGGCAGT GGGCATGGAG AGCAGCAATG TGGAGGTGCT GCACGTGAAC
AAGCCTGACA AGTACCAGCT GCACCTGCAT GAGAGCTGCG TGCTGTCCCT GAAATTTGCT
TACTGTGGTA AATGGTTTGT GAGTACTGGA AAAGATAACC TCCTCAATGC TTGGCGGACC
CCCTATGGAG CCAGCATATT CCAGTCCAAA GAGTCCTCGT CAGTGCTTAG CTGTGACATC
TCTGTGGATG ATAAGTACAT AGTCACTGGC TCGGGGGACA AGAAGGCTAC AGTCTATGAA
GTCATCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252211.1
CDS210..2621
Misc Feature(1)210..605
Misc Feature(2)<1734..2612
Misc Feature(3)1755..2609
Misc Feature(4)order(1755..1757,1809..1811,1836..1841,1887..1892,1947..1949,1959..1961,1977..1982,2022..2027,2076..2078,2091..2093,2109..2114,2151..2153,2202..2204,2217..2219,2235..2240,2265..2270,2328..2330,2340..2342,2358..2363,2394..2399,2448..2450,2463..2465,2481..2486,2517..2522,2574..2576,2586..2588,2604..2609)
Misc Feature(5)1767..1886
Misc Feature(6)1908..2024
Misc Feature(7)2037..2147
Misc Feature(8)2166..2273
Misc Feature(9)2286..2387
Misc Feature(10)2409..2513
Misc Feature(11)2532..2606
Translation

Target ORF information:

RefSeq Version XM_005252154.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252154.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGTT GCAAGCTCAG CATCTTTCTC ATGGCCACGG ACCCCCAGTT
CCCCTTACGC CTCACCCTTC GGGACTTCAG CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT
GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG AGTGGGCAGT CTCACTTGGC AATAAAAGAT
GACAAGAAGC ACCACGATGC AGAGCACCAC AGAGACAGAG AGCCGGGCAC AAGTAATTCC
CTCCTGGTCC CAGACAGTCT AAGAGGCACA GATAAACGCA GAAATGGACC TGAATTTTCC
AATGACATCA AGAAAAGGAA GGTGGATGAT AAGGACTCCA GCCACTATGA CAGTGATGGT
GACAAAAGCG ATGACAACTT AGTTGTGGAT GTGTCTAATG AGGACCCTTC TTCTCCGCGA
GCAAGCCCTG CCCACTCGCC CCGGGAAAAT GGAATCGACA AAAATCGCCT GCTAAAGAAG
GATGCTTCTA GCAGTCCAGC TTCCACGGCC TCCTCGGCAA GTTCCACTTC TTTGAAATCC
AAAGAAATGA GCTTGCATGA AAAAGCCAGC ACGCCTGTTC TGAAATCCAG CACACCAACG
CCTCGGAGCG ACATGCCAAC GCCGGGCACC AGCGCCACTC CAGGCCTCCG TCCAGGTCTC
GGCAAGCCTC CAGCCATAGA CCCCCTCGTT AACCAAGCGG GATTGGATTT TCATTTGGAA
TTGGTACCAG GGAATGGAAA GTTCGGACTG AAAGAGAAAT CTTGGATTTT ATCGAGAAAA
TCTTCTTTTG AGATTAAGTC AGCAGCTGGC TTGAGGACAC CCCTGGCAGT GCCCGGCCCA
TATCCTGCTC CTTTTGGGAT GGTCCCCCAC GCTGGCATGA ACGGCGAGCT GACCAGCCCA
GGCGCTGCCT ACGCCAGTTT ACACAACATG TCGCCCCAGA TGAGCGCCGC AGCCGCCGCG
GCCGCCGTGG TGGCCTACGG GCGCTCCCCC ATGGTGGGGT TTGATCCTCC CCCTCACATG
AGAGTACCTA CCATTCCTCC AAACCTGGCA GGAATCCCTG GGGGGAAACC TGCATACTCC
TTCCACGTTA CTGCAGACGG TCAGATGCAG CCTGTCCCTT TTCCCCCCGA CGCCCTCATC
GGACCCGGAA TCCCCCGGCA TGCTCGCCAG ATCAACACCC TCAACCACGG GGAGGTGGTG
TGCGCTGTGA CCATCAGCAA CCCCACGAGA CACGTGTACA CAGGCGGGAA GGGCTGCGTC
AAGGTCTGGG ACATCAGCCA CCCTGGCAAT AAGAGCCCTG TCTCCCAGCT CGACTGTCTG
AACAGAGACA ATTATATCCG TTCCTGTAAA TTGCTACCCG ATGGCTGCAC TCTCATAGTG
GGAGGGGAAG CCAGTACTTT GTCCATTTGG GACCTGGCGG CTCCAACCCC GCGCATCAAG
GCGGAGCTGA CGTCCTCGGC CCCCGCCTGC TACGCCCTGG CCATCAGCCC CGATTCCAAG
GTCTGCTTCT CATGCTGCAG CGACGGCAAC ATCGCTGTGT GGGATCTGCA CAACCAGACA
CTAGTGAGGC AATTCCAGGG CCACACAGAC GGAGCCAGCT GTATTGACAT TTCTAATGAT
GGCACCAAGC TCTGGACGGG TGGTTTGGAC AACACAGTCA GGTCCTGGGA CCTGCGCGAG
GGGCGGCAGC TGCAGCAGCA CGACTTCACC TCCCAGATCT TCTCCCTGGG GTACTGCCCC
ACCGGGGAGT GGCTGGCAGT GGGCATGGAG AGCAGCAATG TGGAGGTGCT GCACGTGAAC
AAGCCTGACA AGTACCAGCT GCACCTGCAT GAGAGCTGCG TGCTGTCCCT GAAATTTGCT
TACTGTGGTA AATGGTTTGT GAGTACTGGA AAAGATAACC TCCTCAATGC TTGGCGGACC
CCCTATGGAG CCAGCATATT CCAGTCCAAA GAGTCCTCGT CAGTGCTTAG CTGTGACATC
TCTGTGGATG ATAAGTACAT AGTCACTGGC TCGGGGGACA AGAAGGCTAC AGTCTATGAA
GTCATCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43472
Clone ID Related Accession (Same CDS sequence) XM_005252156.2
Accession Version XM_005252156.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGCTGGCTT GAGGACACCC CTGGCAGTGC CCGGCCCATA TCCTGCTCCT
TTTGGGATGG TCCCCCACGC TGGCATGAAC GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC
GCCAGTTTAC ACAACATGTC GCCCCAGATG AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG
GCCTACGGGC GCTCCCCCAT GGTGGGGTTT GATCCTCCCC CTCACATGAG AGTACCTACC
ATTCCTCCAA ACCTGGCAGG AATCCCTGGG GGGAAACCTG CATACTCCTT CCACGTTACT
GCAGACGGTC AGATGCAGCC TGTCCCTTTT CCCCCCGACG CCCTCATCGG ACCCGGAATC
CCCCGGCATG CTCGCCAGAT CAACACCCTC AACCACGGGG AGGTGGTGTG CGCTGTGACC
ATCAGCAACC CCACGAGACA CGTGTACACA GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC
ATCAGCCACC CTGGCAATAA GAGCCCTGTC TCCCAGCTCG ACTGTCTGAA CAGAGACAAT
TATATCCGTT CCTGTAAATT GCTACCCGAT GGCTGCACTC TCATAGTGGG AGGGGAAGCC
AGTACTTTGT CCATTTGGGA CCTGGCGGCT CCAACCCCGC GCATCAAGGC GGAGCTGACG
TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA
TGCTGCAGCG ACGGCAACAT CGCTGTGTGG GATCTGCACA ACCAGACACT AGTGAGGCAA
TTCCAGGGCC ACACAGACGG AGCCAGCTGT ATTGACATTT CTAATGATGG CACCAAGCTC
TGGACGGGTG GTTTGGACAA CACAGTCAGG TCCTGGGACC TGCGCGAGGG GCGGCAGCTG
CAGCAGCACG ACTTCACCTC CCAGATCTTC TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG
CTGGCAGTGG GCATGGAGAG CAGCAATGTG GAGGTGCTGC ACGTGAACAA GCCTGACAAG
TACCAGCTGC ACCTGCATGA GAGCTGCGTG CTGTCCCTGA AATTTGCTTA CTGTGGTAAA
TGGTTTGTGA GTACTGGAAA AGATAACCTC CTCAATGCTT GGCGGACCCC CTATGGAGCC
AGCATATTCC AGTCCAAAGA GTCCTCGTCA GTGCTTAGCT GTGACATCTC TGTGGATGAT
AAGTACATAG TCACTGGCTC GGGGGACAAG AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252213.1
CDS210..2549
Misc Feature(1)210..638
Misc Feature(2)<1662..2540
Misc Feature(3)1683..2537
Misc Feature(4)order(1683..1685,1737..1739,1764..1769,1815..1820,1875..1877,1887..1889,1905..1910,1950..1955,2004..2006,2019..2021,2037..2042,2079..2081,2130..2132,2145..2147,2163..2168,2193..2198,2256..2258,2268..2270,2286..2291,2322..2327,2376..2378,2391..2393,2409..2414,2445..2450,2502..2504,2514..2516,2532..2537)
Misc Feature(5)1695..1814
Misc Feature(6)1836..1952
Misc Feature(7)1965..2075
Misc Feature(8)2094..2201
Misc Feature(9)2214..2315
Misc Feature(10)2337..2441
Misc Feature(11)2460..2534
Translation

Target ORF information:

RefSeq Version XM_005252156.2
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252156.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGCA GTTGCAAGCT
CAGCATCTTT CTCATGGCCA CGGACCCCCA GTTCCCCTTA CGCCTCACCC TTCGGGACTT
CAGCCTCCTG GAATCCCGCC CCTCGGGGGC AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT
CTGAGTGGGC AGTCTCACTT GGCAATAAAA GATGACAAGA AGCACCACGA TGCAGAGCAC
CACAGAGACA GAGAGCCGGG CACAAGTAAT TCCCTCCTGG TCCCAGACAG TCTAAGAGGC
ACAGATAAAC GCAGAAATGG ACCTGAATTT TCCAATGACA TCAAGAAAAG GAAGGTGGAT
GATAAGGACT CCAGCCACTA TGACAGTGAT GGTGACAAAA GCGATGACAA CTTAGTTGTG
GATGTGTCTA ATGAGGACCC TTCTTCTCCG CGAGCAAGCC CTGCCCACTC GCCCCGGGAA
AATGGAATCG ACAAAAATCG CCTGCTAAAG AAGGATGCTT CTAGCAGTCC AGCTTCCACG
GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC
AGCACGCCTG TTCTGAAATC CAGCACACCA ACGCCTCGGA GCGACATGCC AACGCCGGGC
ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT CTCGGCAAGC CTCCAGCCAT AGACCCCCTC
GTTAACCAAG CGGCTGGCTT GAGGACACCC CTGGCAGTGC CCGGCCCATA TCCTGCTCCT
TTTGGGATGG TCCCCCACGC TGGCATGAAC GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC
GCCAGTTTAC ACAACATGTC GCCCCAGATG AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG
GCCTACGGGC GCTCCCCCAT GGTGGGGTTT GATCCTCCCC CTCACATGAG AGTACCTACC
ATTCCTCCAA ACCTGGCAGG AATCCCTGGG GGGAAACCTG CATACTCCTT CCACGTTACT
GCAGACGGTC AGATGCAGCC TGTCCCTTTT CCCCCCGACG CCCTCATCGG ACCCGGAATC
CCCCGGCATG CTCGCCAGAT CAACACCCTC AACCACGGGG AGGTGGTGTG CGCTGTGACC
ATCAGCAACC CCACGAGACA CGTGTACACA GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC
ATCAGCCACC CTGGCAATAA GAGCCCTGTC TCCCAGCTCG ACTGTCTGAA CAGAGACAAT
TATATCCGTT CCTGTAAATT GCTACCCGAT GGCTGCACTC TCATAGTGGG AGGGGAAGCC
AGTACTTTGT CCATTTGGGA CCTGGCGGCT CCAACCCCGC GCATCAAGGC GGAGCTGACG
TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA
TGCTGCAGCG ACGGCAACAT CGCTGTGTGG GATCTGCACA ACCAGACACT AGTGAGGCAA
TTCCAGGGCC ACACAGACGG AGCCAGCTGT ATTGACATTT CTAATGATGG CACCAAGCTC
TGGACGGGTG GTTTGGACAA CACAGTCAGG TCCTGGGACC TGCGCGAGGG GCGGCAGCTG
CAGCAGCACG ACTTCACCTC CCAGATCTTC TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG
CTGGCAGTGG GCATGGAGAG CAGCAATGTG GAGGTGCTGC ACGTGAACAA GCCTGACAAG
TACCAGCTGC ACCTGCATGA GAGCTGCGTG CTGTCCCTGA AATTTGCTTA CTGTGGTAAA
TGGTTTGTGA GTACTGGAAA AGATAACCTC CTCAATGCTT GGCGGACCCC CTATGGAGCC
AGCATATTCC AGTCCAAAGA GTCCTCGTCA GTGCTTAGCT GTGACATCTC TGTGGATGAT
AAGTACATAG TCACTGGCTC GGGGGACAAG AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43473
Clone ID Related Accession (Same CDS sequence) XM_006717259.3
Accession Version XM_006717259.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGCAGCAG
CAGTTGCAAG CTCAGCATCT TTCTCATGGC CACGGACCCC CAGTTCCCCT TACGCCTCAC
CCTTCGGGAC TTCAGCCTCC TGGAATCCCG CCCCTCGGGG GCAGTGCCGG CCTTCTTGCG
CTGTCTAGTG CTCTGAGTGG GCAGTCTCAC TTGGCAATAA AAGATGACAA GAAGCACCAC
GATGCAGAGC ACCACAGAGA CAGAGAGCCG GGCACAAGTA ATTCCCTCCT GGTCCCAGAC
AGTCTAAGAG GCACAGATAA ACGCAGAAAT GGACCTGAAT TTTCCAATGA CATCAAGAAA
AGGAAGGTGG ATGATAAGGA CTCCAGCCAC TATGACAGTG ATGGTGACAA AAGCGATGAC
AACTTAGTTG TGGATGTGTC TAATGAGGAC CCTTCTTCTC CGCGAGCAAG CCCTGCCCAC
TCGCCCCGGG AAAATGGAAT CGACAAAAAT CGCCTGCTAA AGAAGGATGC TTCTAGCAGT
CCAGCTTCCA CGGCCTCCTC GGCAAGTTCC ACTTCTTTGA AATCCAAAGA AATGAGCTTG
CATGAAAAAG CCAGCACGCC TGTTCTGAAA TCCAGCACAC CAACGCCTCG GAGCGACATG
CCAACGCCGG GCACCAGCGC CACTCCAGGC CTCCGTCCAG GTCTCGGCAA GCCTCCAGCC
ATAGACCCCC TCGTTAACCA AGCGGGATTG GATTTTCATT TGGAATTGGT ACCAGGGAAT
GGAAAGTTCG GACTGAAAGA GAAATCTTGG ATTTTATCGA GAAAATCTTC TTTTGAGATT
AAGTCAGCAG CTGGCTTGAG GACACCCCTG GCAGTGCCCG GCCCATATCC TGCTCCTTTT
GGGATGGTCC CCCACGCTGG CATGAACGGC GAGCTGACCA GCCCAGGCGC TGCCTACGCC
AGTTTACACA ACATGTCGCC CCAGATGAGC GCCGCAGCCG CCGCGGCCGC CGTGGTGGCC
TACGGGCGCT CCCCCATGGT GGGGTTTGAT CCTCCCCCTC ACATGAGAGT ACCTACCATT
CCTCCAAACC TGGCAGGAAT CCCTGGGGGG AAACCTGCAT ACTCCTTCCA CGTTACTGCA
GACGGTCAGA TGCAGCCTGT CCCTTTTCCC CCCGACGCCC TCATCGGACC CGGAATCCCC
CGGCATGCTC GCCAGATCAA CACCCTCAAC CACGGGGAGG TGGTGTGCGC TGTGACCATC
AGCAACCCCA CGAGACACGT GTACACAGGC GGGAAGGGCT GCGTCAAGGT CTGGGACATC
AGCCACCCTG GCAATAAGAG CCCTGTCTCC CAGCTCGACT GTCTGAACAG AGACAATTAT
ATCCGTTCCT GTAAATTGCT ACCCGATGGC TGCACTCTCA TAGTGGGAGG GGAAGCCAGT
ACTTTGTCCA TTTGGGACCT GGCGGCTCCA ACCCCGCGCA TCAAGGCGGA GCTGACGTCC
TCGGCCCCCG CCTGCTACGC CCTGGCCATC AGCCCCGATT CCAAGGTCTG CTTCTCATGC
TGCAGCGACG GCAACATCGC TGTGTGGGAT CTGCACAACC AGACACTAGT GAGGCAATTC
CAGGGCCACA CAGACGGAGC CAGCTGTATT GACATTTCTA ATGATGGCAC CAAGCTCTGG
ACGGGTGGTT TGGACAACAC AGTCAGGTCC TGGGACCTGC GCGAGGGGCG GCAGCTGCAG
CAGCACGACT TCACCTCCCA GATCTTCTCC CTGGGGTACT GCCCCACCGG GGAGTGGCTG
GCAGTGGGCA TGGAGAGCAG CAATGTGGAG GTGCTGCACG TGAACAAGCC TGACAAGTAC
CAGCTGCACC TGCATGAGAG CTGCGTGCTG TCCCTGAAAT TTGCTTACTG TGGTAAATGG
TTTGTGAGTA CTGGAAAAGA TAACCTCCTC AATGCTTGGC GGACCCCCTA TGGAGCCAGC
ATATTCCAGT CCAAAGAGTC CTCGTCAGTG CTTAGCTGTG ACATCTCTGT GGATGATAAG
TACATAGTCA CTGGCTCGGG GGACAAGAAG GCTACAGTCT ATGAAGTCAT CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717322.1
CDS61..2337
Misc Feature(1)61..>291
Misc Feature(2)<1450..2328
Misc Feature(3)1471..2325
Misc Feature(4)order(1471..1473,1525..1527,1552..1557,1603..1608,1663..1665,1675..1677,1693..1698,1738..1743,1792..1794,1807..1809,1825..1830,1867..1869,1918..1920,1933..1935,1951..1956,1987..1992,2044..2046,2056..2058,2074..2079,2110..2115,2164..2166,2179..2181,2197..2202,2233..2238,2290..2292,2302..2304,2320..2325)
Misc Feature(5)1483..1602
Misc Feature(6)1624..1740
Misc Feature(7)1753..1863
Misc Feature(8)1882..1989
Misc Feature(9)2002..2103
Misc Feature(10)2125..2229
Misc Feature(11)2248..2322
Translation

Target ORF information:

RefSeq Version XM_006717259.3
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717259.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGCAGCAG
CAGTTGCAAG CTCAGCATCT TTCTCATGGC CACGGACCCC CAGTTCCCCT TACGCCTCAC
CCTTCGGGAC TTCAGCCTCC TGGAATCCCG CCCCTCGGGG GCAGTGCCGG CCTTCTTGCG
CTGTCTAGTG CTCTGAGTGG GCAGTCTCAC TTGGCAATAA AAGATGACAA GAAGCACCAC
GATGCAGAGC ACCACAGAGA CAGAGAGCCG GGCACAAGTA ATTCCCTCCT GGTCCCAGAC
AGTCTAAGAG GCACAGATAA ACGCAGAAAT GGACCTGAAT TTTCCAATGA CATCAAGAAA
AGGAAGGTGG ATGATAAGGA CTCCAGCCAC TATGACAGTG ATGGTGACAA AAGCGATGAC
AACTTAGTTG TGGATGTGTC TAATGAGGAC CCTTCTTCTC CGCGAGCAAG CCCTGCCCAC
TCGCCCCGGG AAAATGGAAT CGACAAAAAT CGCCTGCTAA AGAAGGATGC TTCTAGCAGT
CCAGCTTCCA CGGCCTCCTC GGCAAGTTCC ACTTCTTTGA AATCCAAAGA AATGAGCTTG
CATGAAAAAG CCAGCACGCC TGTTCTGAAA TCCAGCACAC CAACGCCTCG GAGCGACATG
CCAACGCCGG GCACCAGCGC CACTCCAGGC CTCCGTCCAG GTCTCGGCAA GCCTCCAGCC
ATAGACCCCC TCGTTAACCA AGCGGGATTG GATTTTCATT TGGAATTGGT ACCAGGGAAT
GGAAAGTTCG GACTGAAAGA GAAATCTTGG ATTTTATCGA GAAAATCTTC TTTTGAGATT
AAGTCAGCAG CTGGCTTGAG GACACCCCTG GCAGTGCCCG GCCCATATCC TGCTCCTTTT
GGGATGGTCC CCCACGCTGG CATGAACGGC GAGCTGACCA GCCCAGGCGC TGCCTACGCC
AGTTTACACA ACATGTCGCC CCAGATGAGC GCCGCAGCCG CCGCGGCCGC CGTGGTGGCC
TACGGGCGCT CCCCCATGGT GGGGTTTGAT CCTCCCCCTC ACATGAGAGT ACCTACCATT
CCTCCAAACC TGGCAGGAAT CCCTGGGGGG AAACCTGCAT ACTCCTTCCA CGTTACTGCA
GACGGTCAGA TGCAGCCTGT CCCTTTTCCC CCCGACGCCC TCATCGGACC CGGAATCCCC
CGGCATGCTC GCCAGATCAA CACCCTCAAC CACGGGGAGG TGGTGTGCGC TGTGACCATC
AGCAACCCCA CGAGACACGT GTACACAGGC GGGAAGGGCT GCGTCAAGGT CTGGGACATC
AGCCACCCTG GCAATAAGAG CCCTGTCTCC CAGCTCGACT GTCTGAACAG AGACAATTAT
ATCCGTTCCT GTAAATTGCT ACCCGATGGC TGCACTCTCA TAGTGGGAGG GGAAGCCAGT
ACTTTGTCCA TTTGGGACCT GGCGGCTCCA ACCCCGCGCA TCAAGGCGGA GCTGACGTCC
TCGGCCCCCG CCTGCTACGC CCTGGCCATC AGCCCCGATT CCAAGGTCTG CTTCTCATGC
TGCAGCGACG GCAACATCGC TGTGTGGGAT CTGCACAACC AGACACTAGT GAGGCAATTC
CAGGGCCACA CAGACGGAGC CAGCTGTATT GACATTTCTA ATGATGGCAC CAAGCTCTGG
ACGGGTGGTT TGGACAACAC AGTCAGGTCC TGGGACCTGC GCGAGGGGCG GCAGCTGCAG
CAGCACGACT TCACCTCCCA GATCTTCTCC CTGGGGTACT GCCCCACCGG GGAGTGGCTG
GCAGTGGGCA TGGAGAGCAG CAATGTGGAG GTGCTGCACG TGAACAAGCC TGACAAGTAC
CAGCTGCACC TGCATGAGAG CTGCGTGCTG TCCCTGAAAT TTGCTTACTG TGGTAAATGG
TTTGTGAGTA CTGGAAAAGA TAACCTCCTC AATGCTTGGC GGACCCCCTA TGGAGCCAGC
ATATTCCAGT CCAAAGAGTC CTCGTCAGTG CTTAGCTGTG ACATCTCTGT GGATGATAAG
TACATAGTCA CTGGCTCGGG GGACAAGAAG GCTACAGTCT ATGAAGTCAT CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43474
Clone ID Related Accession (Same CDS sequence) XM_006717260.1
Accession Version XM_006717260.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2223bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CAAGTAATTC CCTCCTGGTC
CCAGACAGTC TAAGAGGCAC AGATAAACGC AGAAATGGAC CTGAATTTTC CAATGACATC
AAGAAAAGGA AGGTGGATGA TAAGGACTCC AGCCACTATG ACAGTGATGG TGACAAAAGC
GATGACAACT TAGTTGTGGA TGTGTCTAAT GAGGACCCTT CTTCTCCGCG AGCAAGCCCT
GCCCACTCGC CCCGGGAAAA TGGAATCGAC AAAAATCGCC TGCTAAAGAA GGATGCTTCT
AGCAGTCCAG CTTCCACGGC CTCCTCGGCA AGTTCCACTT CTTTGAAATC CAAAGAAATG
AGCTTGCATG AAAAAGCCAG CACGCCTGTT CTGAAATCCA GCACACCAAC GCCTCGGAGC
GACATGCCAA CGCCGGGCAC CAGCGCCACT CCAGGCCTCC GTCCAGGTCT CGGCAAGCCT
CCAGCCATAG ACCCCCTCGT TAACCAAGCG GGATTGGATT TTCATTTGGA ATTGGTACCA
GGGAATGGAA AGTTCGGACT GAAAGAGAAA TCTTGGATTT TATCGAGAAA ATCTTCTTTT
GAGATTAAGT CAGCAGCTGG CTTGAGGACA CCCCTGGCAG TGCCCGGCCC ATATCCTGCT
CCTTTTGGGA TGGTCCCCCA CGCTGGCATG AACGGCGAGC TGACCAGCCC AGGCGCTGCC
TACGCCAGTT TACACAACAT GTCGCCCCAG ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG
GTGGCCTACG GGCGCTCCCC CATGGTGGGG TTTGATCCTC CCCCTCACAT GAGAGTACCT
ACCATTCCTC CAAACCTGGC AGGAATCCCT GGGGGGAAAC CTGCATACTC CTTCCACGTT
ACTGCAGACG GTCAGATGCA GCCTGTCCCT TTTCCCCCCG ACGCCCTCAT CGGACCCGGA
ATCCCCCGGC ATGCTCGCCA GATCAACACC CTCAACCACG GGGAGGTGGT GTGCGCTGTG
ACCATCAGCA ACCCCACGAG ACACGTGTAC ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG
GACATCAGCC ACCCTGGCAA TAAGAGCCCT GTCTCCCAGC TCGACTGTCT GAACAGAGAC
AATTATATCC GTTCCTGTAA ATTGCTACCC GATGGCTGCA CTCTCATAGT GGGAGGGGAA
GCCAGTACTT TGTCCATTTG GGACCTGGCG GCTCCAACCC CGCGCATCAA GGCGGAGCTG
ACGTCCTCGG CCCCCGCCTG CTACGCCCTG GCCATCAGCC CCGATTCCAA GGTCTGCTTC
TCATGCTGCA GCGACGGCAA CATCGCTGTG TGGGATCTGC ACAACCAGAC ACTAGTGAGG
CAATTCCAGG GCCACACAGA CGGAGCCAGC TGTATTGACA TTTCTAATGA TGGCACCAAG
CTCTGGACGG GTGGTTTGGA CAACACAGTC AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG
CTGCAGCAGC ACGACTTCAC CTCCCAGATC TTCTCCCTGG GGTACTGCCC CACCGGGGAG
TGGCTGGCAG TGGGCATGGA GAGCAGCAAT GTGGAGGTGC TGCACGTGAA CAAGCCTGAC
AAGTACCAGC TGCACCTGCA TGAGAGCTGC GTGCTGTCCC TGAAATTTGC TTACTGTGGT
AAATGGTTTG TGAGTACTGG AAAAGATAAC CTCCTCAATG CTTGGCGGAC CCCCTATGGA
GCCAGCATAT TCCAGTCCAA AGAGTCCTCG TCAGTGCTTA GCTGTGACAT CTCTGTGGAT
GATAAGTACA TAGTCACTGG CTCGGGGGAC AAGAAGGCTA CAGTCTATGA AGTCATCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717323.1
CDS210..2432
Misc Feature(1)210..581
Misc Feature(2)<1545..2423
Misc Feature(3)1566..2420
Misc Feature(4)order(1566..1568,1620..1622,1647..1652,1698..1703,1758..1760,1770..1772,1788..1793,1833..1838,1887..1889,1902..1904,1920..1925,1962..1964,2013..2015,2028..2030,2046..2051,2076..2081,2139..2141,2151..2153,2169..2174,2205..2210,2259..2261,2274..2276,2292..2297,2328..2333,2385..2387,2397..2399,2415..2420)
Misc Feature(5)1578..1697
Misc Feature(6)1719..1835
Misc Feature(7)1848..1958
Misc Feature(8)1977..2084
Misc Feature(9)2097..2198
Misc Feature(10)2220..2324
Misc Feature(11)2343..2417
Translation

Target ORF information:

RefSeq Version XM_006717260.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717260.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CAAGTAATTC CCTCCTGGTC
CCAGACAGTC TAAGAGGCAC AGATAAACGC AGAAATGGAC CTGAATTTTC CAATGACATC
AAGAAAAGGA AGGTGGATGA TAAGGACTCC AGCCACTATG ACAGTGATGG TGACAAAAGC
GATGACAACT TAGTTGTGGA TGTGTCTAAT GAGGACCCTT CTTCTCCGCG AGCAAGCCCT
GCCCACTCGC CCCGGGAAAA TGGAATCGAC AAAAATCGCC TGCTAAAGAA GGATGCTTCT
AGCAGTCCAG CTTCCACGGC CTCCTCGGCA AGTTCCACTT CTTTGAAATC CAAAGAAATG
AGCTTGCATG AAAAAGCCAG CACGCCTGTT CTGAAATCCA GCACACCAAC GCCTCGGAGC
GACATGCCAA CGCCGGGCAC CAGCGCCACT CCAGGCCTCC GTCCAGGTCT CGGCAAGCCT
CCAGCCATAG ACCCCCTCGT TAACCAAGCG GGATTGGATT TTCATTTGGA ATTGGTACCA
GGGAATGGAA AGTTCGGACT GAAAGAGAAA TCTTGGATTT TATCGAGAAA ATCTTCTTTT
GAGATTAAGT CAGCAGCTGG CTTGAGGACA CCCCTGGCAG TGCCCGGCCC ATATCCTGCT
CCTTTTGGGA TGGTCCCCCA CGCTGGCATG AACGGCGAGC TGACCAGCCC AGGCGCTGCC
TACGCCAGTT TACACAACAT GTCGCCCCAG ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG
GTGGCCTACG GGCGCTCCCC CATGGTGGGG TTTGATCCTC CCCCTCACAT GAGAGTACCT
ACCATTCCTC CAAACCTGGC AGGAATCCCT GGGGGGAAAC CTGCATACTC CTTCCACGTT
ACTGCAGACG GTCAGATGCA GCCTGTCCCT TTTCCCCCCG ACGCCCTCAT CGGACCCGGA
ATCCCCCGGC ATGCTCGCCA GATCAACACC CTCAACCACG GGGAGGTGGT GTGCGCTGTG
ACCATCAGCA ACCCCACGAG ACACGTGTAC ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG
GACATCAGCC ACCCTGGCAA TAAGAGCCCT GTCTCCCAGC TCGACTGTCT GAACAGAGAC
AATTATATCC GTTCCTGTAA ATTGCTACCC GATGGCTGCA CTCTCATAGT GGGAGGGGAA
GCCAGTACTT TGTCCATTTG GGACCTGGCG GCTCCAACCC CGCGCATCAA GGCGGAGCTG
ACGTCCTCGG CCCCCGCCTG CTACGCCCTG GCCATCAGCC CCGATTCCAA GGTCTGCTTC
TCATGCTGCA GCGACGGCAA CATCGCTGTG TGGGATCTGC ACAACCAGAC ACTAGTGAGG
CAATTCCAGG GCCACACAGA CGGAGCCAGC TGTATTGACA TTTCTAATGA TGGCACCAAG
CTCTGGACGG GTGGTTTGGA CAACACAGTC AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG
CTGCAGCAGC ACGACTTCAC CTCCCAGATC TTCTCCCTGG GGTACTGCCC CACCGGGGAG
TGGCTGGCAG TGGGCATGGA GAGCAGCAAT GTGGAGGTGC TGCACGTGAA CAAGCCTGAC
AAGTACCAGC TGCACCTGCA TGAGAGCTGC GTGCTGTCCC TGAAATTTGC TTACTGTGGT
AAATGGTTTG TGAGTACTGG AAAAGATAAC CTCCTCAATG CTTGGCGGAC CCCCTATGGA
GCCAGCATAT TCCAGTCCAA AGAGTCCTCG TCAGTGCTTA GCTGTGACAT CTCTGTGGAT
GATAAGTACA TAGTCACTGG CTCGGGGGAC AAGAAGGCTA CAGTCTATGA AGTCATCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43475
Clone ID Related Accession (Same CDS sequence) XM_005252162.1
Accession Version XM_005252162.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2193bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGAGTAATTC CCTCCTGGTC CCAGACAGTC TAAGAGGCAC AGATAAACGC
AGAAATGGAC CTGAATTTTC CAATGACATC AAGAAAAGGA AGGTGGATGA TAAGGACTCC
AGCCACTATG ACAGTGATGG TGACAAAAGC GATGACAACT TAGTTGTGGA TGTGTCTAAT
GAGGACCCTT CTTCTCCGCG AGCAAGCCCT GCCCACTCGC CCCGGGAAAA TGGAATCGAC
AAAAATCGCC TGCTAAAGAA GGATGCTTCT AGCAGTCCAG CTTCCACGGC CTCCTCGGCA
AGTTCCACTT CTTTGAAATC CAAAGAAATG AGCTTGCATG AAAAAGCCAG CACGCCTGTT
CTGAAATCCA GCACACCAAC GCCTCGGAGC GACATGCCAA CGCCGGGCAC CAGCGCCACT
CCAGGCCTCC GTCCAGGTCT CGGCAAGCCT CCAGCCATAG ACCCCCTCGT TAACCAAGCG
GGATTGGATT TTCATTTGGA ATTGGTACCA GGGAATGGAA AGTTCGGACT GAAAGAGAAA
TCTTGGATTT TATCGAGAAA ATCTTCTTTT GAGATTAAGT CAGCAGCTGG CTTGAGGACA
CCCCTGGCAG TGCCCGGCCC ATATCCTGCT CCTTTTGGGA TGGTCCCCCA CGCTGGCATG
AACGGCGAGC TGACCAGCCC AGGCGCTGCC TACGCCAGTT TACACAACAT GTCGCCCCAG
ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG GTGGCCTACG GGCGCTCCCC CATGGTGGGG
TTTGATCCTC CCCCTCACAT GAGAGTACCT ACCATTCCTC CAAACCTGGC AGGAATCCCT
GGGGGGAAAC CTGCATACTC CTTCCACGTT ACTGCAGACG GTCAGATGCA GCCTGTCCCT
TTTCCCCCCG ACGCCCTCAT CGGACCCGGA ATCCCCCGGC ATGCTCGCCA GATCAACACC
CTCAACCACG GGGAGGTGGT GTGCGCTGTG ACCATCAGCA ACCCCACGAG ACACGTGTAC
ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG GACATCAGCC ACCCTGGCAA TAAGAGCCCT
GTCTCCCAGC TCGACTGTCT GAACAGAGAC AATTATATCC GTTCCTGTAA ATTGCTACCC
GATGGCTGCA CTCTCATAGT GGGAGGGGAA GCCAGTACTT TGTCCATTTG GGACCTGGCG
GCTCCAACCC CGCGCATCAA GGCGGAGCTG ACGTCCTCGG CCCCCGCCTG CTACGCCCTG
GCCATCAGCC CCGATTCCAA GGTCTGCTTC TCATGCTGCA GCGACGGCAA CATCGCTGTG
TGGGATCTGC ACAACCAGAC ACTAGTGAGG CAATTCCAGG GCCACACAGA CGGAGCCAGC
TGTATTGACA TTTCTAATGA TGGCACCAAG CTCTGGACGG GTGGTTTGGA CAACACAGTC
AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG CTGCAGCAGC ACGACTTCAC CTCCCAGATC
TTCTCCCTGG GGTACTGCCC CACCGGGGAG TGGCTGGCAG TGGGCATGGA GAGCAGCAAT
GTGGAGGTGC TGCACGTGAA CAAGCCTGAC AAGTACCAGC TGCACCTGCA TGAGAGCTGC
GTGCTGTCCC TGAAATTTGC TTACTGTGGT AAATGGTTTG TGAGTACTGG AAAAGATAAC
CTCCTCAATG CTTGGCGGAC CCCCTATGGA GCCAGCATAT TCCAGTCCAA AGAGTCCTCG
TCAGTGCTTA GCTGTGACAT CTCTGTGGAT GATAAGTACA TAGTCACTGG CTCGGGGGAC
AAGAAGGCTA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252219.1
CDS210..2402
Misc Feature(1)210..581
Misc Feature(2)<1515..2393
Misc Feature(3)1536..2390
Misc Feature(4)order(1536..1538,1590..1592,1617..1622,1668..1673,1728..1730,1740..1742,1758..1763,1803..1808,1857..1859,1872..1874,1890..1895,1932..1934,1983..1985,1998..2000,2016..2021,2046..2051,2109..2111,2121..2123,2139..2144,2175..2180,2229..2231,2244..2246,2262..2267,2298..2303,2355..2357,2367..2369,2385..2390)
Misc Feature(5)1548..1667
Misc Feature(6)1689..1805
Misc Feature(7)1818..1928
Misc Feature(8)1947..2054
Misc Feature(9)2067..2168
Misc Feature(10)2190..2294
Misc Feature(11)2313..2387
Translation

Target ORF information:

RefSeq Version XM_005252162.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252162.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGAGTAATTC CCTCCTGGTC CCAGACAGTC TAAGAGGCAC AGATAAACGC
AGAAATGGAC CTGAATTTTC CAATGACATC AAGAAAAGGA AGGTGGATGA TAAGGACTCC
AGCCACTATG ACAGTGATGG TGACAAAAGC GATGACAACT TAGTTGTGGA TGTGTCTAAT
GAGGACCCTT CTTCTCCGCG AGCAAGCCCT GCCCACTCGC CCCGGGAAAA TGGAATCGAC
AAAAATCGCC TGCTAAAGAA GGATGCTTCT AGCAGTCCAG CTTCCACGGC CTCCTCGGCA
AGTTCCACTT CTTTGAAATC CAAAGAAATG AGCTTGCATG AAAAAGCCAG CACGCCTGTT
CTGAAATCCA GCACACCAAC GCCTCGGAGC GACATGCCAA CGCCGGGCAC CAGCGCCACT
CCAGGCCTCC GTCCAGGTCT CGGCAAGCCT CCAGCCATAG ACCCCCTCGT TAACCAAGCG
GGATTGGATT TTCATTTGGA ATTGGTACCA GGGAATGGAA AGTTCGGACT GAAAGAGAAA
TCTTGGATTT TATCGAGAAA ATCTTCTTTT GAGATTAAGT CAGCAGCTGG CTTGAGGACA
CCCCTGGCAG TGCCCGGCCC ATATCCTGCT CCTTTTGGGA TGGTCCCCCA CGCTGGCATG
AACGGCGAGC TGACCAGCCC AGGCGCTGCC TACGCCAGTT TACACAACAT GTCGCCCCAG
ATGAGCGCCG CAGCCGCCGC GGCCGCCGTG GTGGCCTACG GGCGCTCCCC CATGGTGGGG
TTTGATCCTC CCCCTCACAT GAGAGTACCT ACCATTCCTC CAAACCTGGC AGGAATCCCT
GGGGGGAAAC CTGCATACTC CTTCCACGTT ACTGCAGACG GTCAGATGCA GCCTGTCCCT
TTTCCCCCCG ACGCCCTCAT CGGACCCGGA ATCCCCCGGC ATGCTCGCCA GATCAACACC
CTCAACCACG GGGAGGTGGT GTGCGCTGTG ACCATCAGCA ACCCCACGAG ACACGTGTAC
ACAGGCGGGA AGGGCTGCGT CAAGGTCTGG GACATCAGCC ACCCTGGCAA TAAGAGCCCT
GTCTCCCAGC TCGACTGTCT GAACAGAGAC AATTATATCC GTTCCTGTAA ATTGCTACCC
GATGGCTGCA CTCTCATAGT GGGAGGGGAA GCCAGTACTT TGTCCATTTG GGACCTGGCG
GCTCCAACCC CGCGCATCAA GGCGGAGCTG ACGTCCTCGG CCCCCGCCTG CTACGCCCTG
GCCATCAGCC CCGATTCCAA GGTCTGCTTC TCATGCTGCA GCGACGGCAA CATCGCTGTG
TGGGATCTGC ACAACCAGAC ACTAGTGAGG CAATTCCAGG GCCACACAGA CGGAGCCAGC
TGTATTGACA TTTCTAATGA TGGCACCAAG CTCTGGACGG GTGGTTTGGA CAACACAGTC
AGGTCCTGGG ACCTGCGCGA GGGGCGGCAG CTGCAGCAGC ACGACTTCAC CTCCCAGATC
TTCTCCCTGG GGTACTGCCC CACCGGGGAG TGGCTGGCAG TGGGCATGGA GAGCAGCAAT
GTGGAGGTGC TGCACGTGAA CAAGCCTGAC AAGTACCAGC TGCACCTGCA TGAGAGCTGC
GTGCTGTCCC TGAAATTTGC TTACTGTGGT AAATGGTTTG TGAGTACTGG AAAAGATAAC
CTCCTCAATG CTTGGCGGAC CCCCTATGGA GCCAGCATAT TCCAGTCCAA AGAGTCCTCG
TCAGTGCTTA GCTGTGACAT CTCTGTGGAT GATAAGTACA TAGTCACTGG CTCGGGGGAC
AAGAAGGCTA CAGTCTATGA AGTCATCTAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43476
Clone ID Related Accession (Same CDS sequence) XM_005252163.2
Accession Version XM_005252163.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X16
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGAGTAATTC CCTCCTGGTC CCAGACAGTC TAAGAGGCAC AGATAAACGC
AGAAATGGAC CTGAATTTTC CAATGACATC AAGAAAAGGA AGGTGGATGA TAAGGACTCC
AGCCACTATG ACAGTGATGG TGACAAAAGC GATGACAACT TAGTTGTGGA TGTGTCTAAT
GAGGACCCTT CTTCTCCGCG AGCAAGCCCT GCCCACTCGC CCCGGGAAAA TGGAATCGAC
AAAAATCGCC TGCTAAAGAA GGATGCTTCT AGCAGTCCAG CTTCCACGGC CTCCTCGGCA
AGTTCCACTT CTTTGAAATC CAAAGAAATG AGCTTGCATG AAAAAGCCAG CACGCCTGTT
CTGAAATCCA GCACACCAAC GCCTCGGAGC GACATGCCAA CGCCGGGCAC CAGCGCCACT
CCAGGCCTCC GTCCAGGTCT CGGCAAGCCT CCAGCCATAG ACCCCCTCGT TAACCAAGCG
GCAGCTGGCT TGAGGACACC CCTGGCAGTG CCCGGCCCAT ATCCTGCTCC TTTTGGGATG
GTCCCCCACG CTGGCATGAA CGGCGAGCTG ACCAGCCCAG GCGCTGCCTA CGCCAGTTTA
CACAACATGT CGCCCCAGAT GAGCGCCGCA GCCGCCGCGG CCGCCGTGGT GGCCTACGGG
CGCTCCCCCA TGGTGGGGTT TGATCCTCCC CCTCACATGA GAGTACCTAC CATTCCTCCA
AACCTGGCAG GAATCCCTGG GGGGAAACCT GCATACTCCT TCCACGTTAC TGCAGACGGT
CAGATGCAGC CTGTCCCTTT TCCCCCCGAC GCCCTCATCG GACCCGGAAT CCCCCGGCAT
GCTCGCCAGA TCAACACCCT CAACCACGGG GAGGTGGTGT GCGCTGTGAC CATCAGCAAC
CCCACGAGAC ACGTGTACAC AGGCGGGAAG GGCTGCGTCA AGGTCTGGGA CATCAGCCAC
CCTGGCAATA AGAGCCCTGT CTCCCAGCTC GACTGTCTGA ACAGAGACAA TTATATCCGT
TCCTGTAAAT TGCTACCCGA TGGCTGCACT CTCATAGTGG GAGGGGAAGC CAGTACTTTG
TCCATTTGGG ACCTGGCGGC TCCAACCCCG CGCATCAAGG CGGAGCTGAC GTCCTCGGCC
CCCGCCTGCT ACGCCCTGGC CATCAGCCCC GATTCCAAGG TCTGCTTCTC ATGCTGCAGC
GACGGCAACA TCGCTGTGTG GGATCTGCAC AACCAGACAC TAGTGAGGCA ATTCCAGGGC
CACACAGACG GAGCCAGCTG TATTGACATT TCTAATGATG GCACCAAGCT CTGGACGGGT
GGTTTGGACA ACACAGTCAG GTCCTGGGAC CTGCGCGAGG GGCGGCAGCT GCAGCAGCAC
GACTTCACCT CCCAGATCTT CTCCCTGGGG TACTGCCCCA CCGGGGAGTG GCTGGCAGTG
GGCATGGAGA GCAGCAATGT GGAGGTGCTG CACGTGAACA AGCCTGACAA GTACCAGCTG
CACCTGCATG AGAGCTGCGT GCTGTCCCTG AAATTTGCTT ACTGTGGTAA ATGGTTTGTG
AGTACTGGAA AAGATAACCT CCTCAATGCT TGGCGGACCC CCTATGGAGC CAGCATATTC
CAGTCCAAAG AGTCCTCGTC AGTGCTTAGC TGTGACATCT CTGTGGATGA TAAGTACATA
GTCACTGGCT CGGGGGACAA GAAGGCTACA GTCTATGAAG TCATCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252220.1
CDS210..2300
Misc Feature(1)210..581
Misc Feature(2)<1413..2291
Misc Feature(3)1434..2288
Misc Feature(4)order(1434..1436,1488..1490,1515..1520,1566..1571,1626..1628,1638..1640,1656..1661,1701..1706,1755..1757,1770..1772,1788..1793,1830..1832,1881..1883,1896..1898,1914..1919,1944..1949,2007..2009,2019..2021,2037..2042,2073..2078,2127..2129,2142..2144,2160..2165,2196..2201,2253..2255,2265..2267,2283..2288)
Misc Feature(5)1446..1565
Misc Feature(6)1587..1703
Misc Feature(7)1716..1826
Misc Feature(8)1845..1952
Misc Feature(9)1965..2066
Misc Feature(10)2088..2192
Misc Feature(11)2211..2285
Translation

Target ORF information:

RefSeq Version XM_005252163.2
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252163.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGAGTAATTC CCTCCTGGTC CCAGACAGTC TAAGAGGCAC AGATAAACGC
AGAAATGGAC CTGAATTTTC CAATGACATC AAGAAAAGGA AGGTGGATGA TAAGGACTCC
AGCCACTATG ACAGTGATGG TGACAAAAGC GATGACAACT TAGTTGTGGA TGTGTCTAAT
GAGGACCCTT CTTCTCCGCG AGCAAGCCCT GCCCACTCGC CCCGGGAAAA TGGAATCGAC
AAAAATCGCC TGCTAAAGAA GGATGCTTCT AGCAGTCCAG CTTCCACGGC CTCCTCGGCA
AGTTCCACTT CTTTGAAATC CAAAGAAATG AGCTTGCATG AAAAAGCCAG CACGCCTGTT
CTGAAATCCA GCACACCAAC GCCTCGGAGC GACATGCCAA CGCCGGGCAC CAGCGCCACT
CCAGGCCTCC GTCCAGGTCT CGGCAAGCCT CCAGCCATAG ACCCCCTCGT TAACCAAGCG
GCAGCTGGCT TGAGGACACC CCTGGCAGTG CCCGGCCCAT ATCCTGCTCC TTTTGGGATG
GTCCCCCACG CTGGCATGAA CGGCGAGCTG ACCAGCCCAG GCGCTGCCTA CGCCAGTTTA
CACAACATGT CGCCCCAGAT GAGCGCCGCA GCCGCCGCGG CCGCCGTGGT GGCCTACGGG
CGCTCCCCCA TGGTGGGGTT TGATCCTCCC CCTCACATGA GAGTACCTAC CATTCCTCCA
AACCTGGCAG GAATCCCTGG GGGGAAACCT GCATACTCCT TCCACGTTAC TGCAGACGGT
CAGATGCAGC CTGTCCCTTT TCCCCCCGAC GCCCTCATCG GACCCGGAAT CCCCCGGCAT
GCTCGCCAGA TCAACACCCT CAACCACGGG GAGGTGGTGT GCGCTGTGAC CATCAGCAAC
CCCACGAGAC ACGTGTACAC AGGCGGGAAG GGCTGCGTCA AGGTCTGGGA CATCAGCCAC
CCTGGCAATA AGAGCCCTGT CTCCCAGCTC GACTGTCTGA ACAGAGACAA TTATATCCGT
TCCTGTAAAT TGCTACCCGA TGGCTGCACT CTCATAGTGG GAGGGGAAGC CAGTACTTTG
TCCATTTGGG ACCTGGCGGC TCCAACCCCG CGCATCAAGG CGGAGCTGAC GTCCTCGGCC
CCCGCCTGCT ACGCCCTGGC CATCAGCCCC GATTCCAAGG TCTGCTTCTC ATGCTGCAGC
GACGGCAACA TCGCTGTGTG GGATCTGCAC AACCAGACAC TAGTGAGGCA ATTCCAGGGC
CACACAGACG GAGCCAGCTG TATTGACATT TCTAATGATG GCACCAAGCT CTGGACGGGT
GGTTTGGACA ACACAGTCAG GTCCTGGGAC CTGCGCGAGG GGCGGCAGCT GCAGCAGCAC
GACTTCACCT CCCAGATCTT CTCCCTGGGG TACTGCCCCA CCGGGGAGTG GCTGGCAGTG
GGCATGGAGA GCAGCAATGT GGAGGTGCTG CACGTGAACA AGCCTGACAA GTACCAGCTG
CACCTGCATG AGAGCTGCGT GCTGTCCCTG AAATTTGCTT ACTGTGGTAA ATGGTTTGTG
AGTACTGGAA AAGATAACCT CCTCAATGCT TGGCGGACCC CCTATGGAGC CAGCATATTC
CAGTCCAAAG AGTCCTCGTC AGTGCTTAGC TGTGACATCT CTGTGGATGA TAAGTACATA
GTCACTGGCT CGGGGGACAA GAAGGCTACA GTCTATGAAG TCATCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43477
Clone ID Related Accession (Same CDS sequence) XM_006717261.2
Accession Version XM_006717261.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006717261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCAGAAT TGAATGCCAT CATCGGGGTA CGTGGCCTAC CAGGTCTACC TCCTACACAG 
CAGCAGTTGC AAGCTCAGCA TCTTTCTCAT GGCCACGGAC CCCCAGTTCC CCTTACGCCT
CACCCTTCGG GACTTCAGCC TCCTGGAATC CCGCCCCTCG GGGGCAGTGC CGGCCTTCTT
GCGCTGTCTA GTGCTCTGAG TGGGCAGTCT CACTTGGCAA TAAAAGATGA CAAGAAGCAC
CACGATGCAG AGCACCACAG AGACAGAGAG CCGGGCACAA GTAATTCCCT CCTGGTCCCA
GACAGTCTAA GAGGCACAGA TAAACGCAGA AATGGACCTG AATTTTCCAA TGACATCAAG
AAAAGGAAGG TGGATGATAA GGACTCCAGC CACTATGACA GTGATGGTGA CAAAAGCGAT
GACAACTTAG TTGTGGATGT GTCTAATGAG GACCCTTCTT CTCCGCGAGC AAGCCCTGCC
CACTCGCCCC GGGAAAATGG AATCGACAAA AATCGCCTGC TAAAGAAGGA TGCTTCTAGC
AGTCCAGCTT CCACGGCCTC CTCGGCAAGT TCCACTTCTT TGAAATCCAA AGAAATGAGC
TTGCATGAAA AAGCCAGCAC GCCTGTTCTG AAATCCAGCA CACCAACGCC TCGGAGCGAC
ATGCCAACGC CGGGCACCAG CGCCACTCCA GGCCTCCGTC CAGGTCTCGG CAAGCCTCCA
GCCATAGACC CCCTCGTTAA CCAAGCGGGA TTGGATTTTC ATTTGGAATT GGTACCAGGG
AATGGAAAGT TCGGACTGAA AGAGAAATCT TGGATTTTAT CGAGAAAATC TTCTTTTGAG
ATTAAGTCAG CAGCTGGCTT GAGGACACCC CTGGCAGTGC CCGGCCCATA TCCTGCTCCT
TTTGGGATGG TCCCCCACGC TGGCATGAAC GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC
GCCAGTTTAC ACAACATGTC GCCCCAGATG AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG
GCCTACGGGC GCTCCCCCAT GGTGGGGTTT GATCCTCCCC CTCACATGAG AGTACCTACC
ATTCCTCCAA ACCTGGCAGG AATCCCTGGG GGGAAACCTG CATACTCCTT CCACGTTACT
GCAGACGGTC AGATGCAGCC TGTCCCTTTT CCCCCCGACG CCCTCATCGG ACCCGGAATC
CCCCGGCATG CTCGCCAGAT CAACACCCTC AACCACGGGG AGGTGGTGTG CGCTGTGACC
ATCAGCAACC CCACGAGACA CGTGTACACA GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC
ATCAGCCACC CTGGCAATAA GAGCCCTGTC TCCCAGCTCG ACTGTCTGAA CAGAGACAAT
TATATCCGTT CCTGTAAATT GCTACCCGAT GGCTGCACTC TCATAGTGGG AGGGGAAGCC
AGTACTTTGT CCATTTGGGA CCTGGCGGCT CCAACCCCGC GCATCAAGGC GGAGCTGACG
TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA
TGCTGCAGCG ACGGCAACAT CGCTGTGTGG GATCTGCACA ACCAGACACT AGTGAGGCAA
TTCCAGGGCC ACACAGACGG AGCCAGCTGT ATTGACATTT CTAATGATGG CACCAAGCTC
TGGACGGGTG GTTTGGACAA CACAGTCAGG TCCTGGGACC TGCGCGAGGG GCGGCAGCTG
CAGCAGCACG ACTTCACCTC CCAGATCTTC TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG
CTGGCAGTGG GCATGGAGAG CAGCAATGTG GAGGTGCTGC ACGTGAACAA GCCTGACAAG
TACCAGCTGC ACCTGCATGA GAGCTGCGTG CTGTCCCTGA AATTTGCTTA CTGTGGTAAA
TGGTTTGTGA GTACTGGAAA AGATAACCTC CTCAATGCTT GGCGGACCCC CTATGGAGCC
AGCATATTCC AGTCCAAAGA GTCCTCGTCA GTGCTTAGCT GTGACATCTC TGTGGATGAT
AAGTACATAG TCACTGGCTC GGGGGACAAG AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717324.1
CDS267..2366
Misc Feature(1)<1497..2357
Misc Feature(2)1500..2354
Misc Feature(3)order(1500..1502,1554..1556,1581..1586,1632..1637,1692..1694,1704..1706,1722..1727,1767..1772,1821..1823,1836..1838,1854..1859,1896..1898,1947..1949,1962..1964,1980..1985,2016..2021,2073..2075,2085..2087,2103..2108,2139..2144,2193..2195,2208..2210,2226..2231,2262..2267,2319..2321,2331..2333,2349..2354)
Misc Feature(4)1512..1631
Misc Feature(5)1653..1769
Misc Feature(6)1782..1892
Misc Feature(7)1911..2018
Misc Feature(8)2031..2132
Misc Feature(9)2154..2258
Misc Feature(10)2277..2351
Translation

Target ORF information:

RefSeq Version XM_006717261.2
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717261.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCAGAAT TGAATGCCAT CATCGGGGTA CGTGGCCTAC CAGGTCTACC TCCTACACAG 
CAGCAGTTGC AAGCTCAGCA TCTTTCTCAT GGCCACGGAC CCCCAGTTCC CCTTACGCCT
CACCCTTCGG GACTTCAGCC TCCTGGAATC CCGCCCCTCG GGGGCAGTGC CGGCCTTCTT
GCGCTGTCTA GTGCTCTGAG TGGGCAGTCT CACTTGGCAA TAAAAGATGA CAAGAAGCAC
CACGATGCAG AGCACCACAG AGACAGAGAG CCGGGCACAA GTAATTCCCT CCTGGTCCCA
GACAGTCTAA GAGGCACAGA TAAACGCAGA AATGGACCTG AATTTTCCAA TGACATCAAG
AAAAGGAAGG TGGATGATAA GGACTCCAGC CACTATGACA GTGATGGTGA CAAAAGCGAT
GACAACTTAG TTGTGGATGT GTCTAATGAG GACCCTTCTT CTCCGCGAGC AAGCCCTGCC
CACTCGCCCC GGGAAAATGG AATCGACAAA AATCGCCTGC TAAAGAAGGA TGCTTCTAGC
AGTCCAGCTT CCACGGCCTC CTCGGCAAGT TCCACTTCTT TGAAATCCAA AGAAATGAGC
TTGCATGAAA AAGCCAGCAC GCCTGTTCTG AAATCCAGCA CACCAACGCC TCGGAGCGAC
ATGCCAACGC CGGGCACCAG CGCCACTCCA GGCCTCCGTC CAGGTCTCGG CAAGCCTCCA
GCCATAGACC CCCTCGTTAA CCAAGCGGGA TTGGATTTTC ATTTGGAATT GGTACCAGGG
AATGGAAAGT TCGGACTGAA AGAGAAATCT TGGATTTTAT CGAGAAAATC TTCTTTTGAG
ATTAAGTCAG CAGCTGGCTT GAGGACACCC CTGGCAGTGC CCGGCCCATA TCCTGCTCCT
TTTGGGATGG TCCCCCACGC TGGCATGAAC GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC
GCCAGTTTAC ACAACATGTC GCCCCAGATG AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG
GCCTACGGGC GCTCCCCCAT GGTGGGGTTT GATCCTCCCC CTCACATGAG AGTACCTACC
ATTCCTCCAA ACCTGGCAGG AATCCCTGGG GGGAAACCTG CATACTCCTT CCACGTTACT
GCAGACGGTC AGATGCAGCC TGTCCCTTTT CCCCCCGACG CCCTCATCGG ACCCGGAATC
CCCCGGCATG CTCGCCAGAT CAACACCCTC AACCACGGGG AGGTGGTGTG CGCTGTGACC
ATCAGCAACC CCACGAGACA CGTGTACACA GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC
ATCAGCCACC CTGGCAATAA GAGCCCTGTC TCCCAGCTCG ACTGTCTGAA CAGAGACAAT
TATATCCGTT CCTGTAAATT GCTACCCGAT GGCTGCACTC TCATAGTGGG AGGGGAAGCC
AGTACTTTGT CCATTTGGGA CCTGGCGGCT CCAACCCCGC GCATCAAGGC GGAGCTGACG
TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA
TGCTGCAGCG ACGGCAACAT CGCTGTGTGG GATCTGCACA ACCAGACACT AGTGAGGCAA
TTCCAGGGCC ACACAGACGG AGCCAGCTGT ATTGACATTT CTAATGATGG CACCAAGCTC
TGGACGGGTG GTTTGGACAA CACAGTCAGG TCCTGGGACC TGCGCGAGGG GCGGCAGCTG
CAGCAGCACG ACTTCACCTC CCAGATCTTC TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG
CTGGCAGTGG GCATGGAGAG CAGCAATGTG GAGGTGCTGC ACGTGAACAA GCCTGACAAG
TACCAGCTGC ACCTGCATGA GAGCTGCGTG CTGTCCCTGA AATTTGCTTA CTGTGGTAAA
TGGTTTGTGA GTACTGGAAA AGATAACCTC CTCAATGCTT GGCGGACCCC CTATGGAGCC
AGCATATTCC AGTCCAAAGA GTCCTCGTCA GTGCTTAGCT GTGACATCTC TGTGGATGAT
AAGTACATAG TCACTGGCTC GGGGGACAAG AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43478
Clone ID Related Accession (Same CDS sequence) XM_006717262.1
Accession Version XM_006717262.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT
CTCGGCAAGC CTCCAGCCAT AGACCCCCTC GTTAACCAAG CGGCTGGCTT GAGGACACCC
CTGGCAGTGC CCGGCCCATA TCCTGCTCCT TTTGGGATGG TCCCCCACGC TGGCATGAAC
GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC GCCAGTTTAC ACAACATGTC GCCCCAGATG
AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG GCCTACGGGC GCTCCCCCAT GGTGGGGTTT
GATCCTCCCC CTCACATGAG AGTACCTACC ATTCCTCCAA ACCTGGCAGG AATCCCTGGG
GGGAAACCTG CATACTCCTT CCACGTTACT GCAGACGGTC AGATGCAGCC TGTCCCTTTT
CCCCCCGACG CCCTCATCGG ACCCGGAATC CCCCGGCATG CTCGCCAGAT CAACACCCTC
AACCACGGGG AGGTGGTGTG CGCTGTGACC ATCAGCAACC CCACGAGACA CGTGTACACA
GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC ATCAGCCACC CTGGCAATAA GAGCCCTGTC
TCCCAGCTCG ACTGTCTGAA CAGAGACAAT TATATCCGTT CCTGTAAATT GCTACCCGAT
GGCTGCACTC TCATAGTGGG AGGGGAAGCC AGTACTTTGT CCATTTGGGA CCTGGCGGCT
CCAACCCCGC GCATCAAGGC GGAGCTGACG TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC
ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA TGCTGCAGCG ACGGCAACAT CGCTGTGTGG
GATCTGCACA ACCAGACACT AGTGAGGCAA TTCCAGGGCC ACACAGACGG AGCCAGCTGT
ATTGACATTT CTAATGATGG CACCAAGCTC TGGACGGGTG GTTTGGACAA CACAGTCAGG
TCCTGGGACC TGCGCGAGGG GCGGCAGCTG CAGCAGCACG ACTTCACCTC CCAGATCTTC
TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG CTGGCAGTGG GCATGGAGAG CAGCAATGTG
GAGGTGCTGC ACGTGAACAA GCCTGACAAG TACCAGCTGC ACCTGCATGA GAGCTGCGTG
CTGTCCCTGA AATTTGCTTA CTGTGGTAAA TGGTTTGTGA GTACTGGAAA AGATAACCTC
CTCAATGCTT GGCGGACCCC CTATGGAGCC AGCATATTCC AGTCCAAAGA GTCCTCGTCA
GTGCTTAGCT GTGACATCTC TGTGGATGAT AAGTACATAG TCACTGGCTC GGGGGACAAG
AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717325.1
CDS210..2519
Misc Feature(1)210..608
Misc Feature(2)<1632..2510
Misc Feature(3)1653..2507
Misc Feature(4)order(1653..1655,1707..1709,1734..1739,1785..1790,1845..1847,1857..1859,1875..1880,1920..1925,1974..1976,1989..1991,2007..2012,2049..2051,2100..2102,2115..2117,2133..2138,2163..2168,2226..2228,2238..2240,2256..2261,2292..2297,2346..2348,2361..2363,2379..2384,2415..2420,2472..2474,2484..2486,2502..2507)
Misc Feature(5)1665..1784
Misc Feature(6)1806..1922
Misc Feature(7)1935..2045
Misc Feature(8)2064..2171
Misc Feature(9)2184..2285
Misc Feature(10)2307..2411
Misc Feature(11)2430..2504
Translation

Target ORF information:

RefSeq Version XM_006717262.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717262.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGCA GTTGCAAGCT CAGCATCTTT CTCATGGCCA CGGACCCCCA
GTTCCCCTTA CGCCTCACCC TTCGGGACTT CAGCCTCCTG GAATCCCGCC CCTCGGGGGC
AGTGCCGGCC TTCTTGCGCT GTCTAGTGCT CTGAGTGGGC AGTCTCACTT GGCAATAAAA
GATGACAAGA AGCACCACGA TGCAGAGCAC CACAGAGACA GAGAGCCGGG CACAAGTAAT
TCCCTCCTGG TCCCAGACAG TCTAAGAGGC ACAGATAAAC GCAGAAATGG ACCTGAATTT
TCCAATGACA TCAAGAAAAG GAAGGTGGAT GATAAGGACT CCAGCCACTA TGACAGTGAT
GGTGACAAAA GCGATGACAA CTTAGTTGTG GATGTGTCTA ATGAGGACCC TTCTTCTCCG
CGAGCAAGCC CTGCCCACTC GCCCCGGGAA AATGGAATCG ACAAAAATCG CCTGCTAAAG
AAGGATGCTT CTAGCAGTCC AGCTTCCACG GCCTCCTCGG CAAGTTCCAC TTCTTTGAAA
TCCAAAGAAA TGAGCTTGCA TGAAAAAGCC AGCACGCCTG TTCTGAAATC CAGCACACCA
ACGCCTCGGA GCGACATGCC AACGCCGGGC ACCAGCGCCA CTCCAGGCCT CCGTCCAGGT
CTCGGCAAGC CTCCAGCCAT AGACCCCCTC GTTAACCAAG CGGCTGGCTT GAGGACACCC
CTGGCAGTGC CCGGCCCATA TCCTGCTCCT TTTGGGATGG TCCCCCACGC TGGCATGAAC
GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC GCCAGTTTAC ACAACATGTC GCCCCAGATG
AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG GCCTACGGGC GCTCCCCCAT GGTGGGGTTT
GATCCTCCCC CTCACATGAG AGTACCTACC ATTCCTCCAA ACCTGGCAGG AATCCCTGGG
GGGAAACCTG CATACTCCTT CCACGTTACT GCAGACGGTC AGATGCAGCC TGTCCCTTTT
CCCCCCGACG CCCTCATCGG ACCCGGAATC CCCCGGCATG CTCGCCAGAT CAACACCCTC
AACCACGGGG AGGTGGTGTG CGCTGTGACC ATCAGCAACC CCACGAGACA CGTGTACACA
GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC ATCAGCCACC CTGGCAATAA GAGCCCTGTC
TCCCAGCTCG ACTGTCTGAA CAGAGACAAT TATATCCGTT CCTGTAAATT GCTACCCGAT
GGCTGCACTC TCATAGTGGG AGGGGAAGCC AGTACTTTGT CCATTTGGGA CCTGGCGGCT
CCAACCCCGC GCATCAAGGC GGAGCTGACG TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC
ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA TGCTGCAGCG ACGGCAACAT CGCTGTGTGG
GATCTGCACA ACCAGACACT AGTGAGGCAA TTCCAGGGCC ACACAGACGG AGCCAGCTGT
ATTGACATTT CTAATGATGG CACCAAGCTC TGGACGGGTG GTTTGGACAA CACAGTCAGG
TCCTGGGACC TGCGCGAGGG GCGGCAGCTG CAGCAGCACG ACTTCACCTC CCAGATCTTC
TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG CTGGCAGTGG GCATGGAGAG CAGCAATGTG
GAGGTGCTGC ACGTGAACAA GCCTGACAAG TACCAGCTGC ACCTGCATGA GAGCTGCGTG
CTGTCCCTGA AATTTGCTTA CTGTGGTAAA TGGTTTGTGA GTACTGGAAA AGATAACCTC
CTCAATGCTT GGCGGACCCC CTATGGAGCC AGCATATTCC AGTCCAAAGA GTCCTCGTCA
GTGCTTAGCT GTGACATCTC TGTGGATGAT AAGTACATAG TCACTGGCTC GGGGGACAAG
AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43479
Clone ID Related Accession (Same CDS sequence) XM_006717263.1
Accession Version XM_006717263.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2307bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X17
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGTT GCAAGCTCAG CATCTTTCTC ATGGCCACGG ACCCCCAGTT
CCCCTTACGC CTCACCCTTC GGGACTTCAG CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT
GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG AGTGGGCAGT CTCACTTGGC AATAAAAGAT
GACAAGAAGC ACCACGATGC AGAGCACCAC AGAGACAGAG AGCCGGGCAC AAGTAATTCC
CTCCTGGTCC CAGACAGTCT AAGAGGCACA GATAAACGCA GAAATGGACC TGAATTTTCC
AATGACATCA AGAAAAGGAA GGTGGATGAT AAGGACTCCA GCCACTATGA CAGTGATGGT
GACAAAAGCG ATGACAACTT AGTTGTGGAT GTGTCTAATG AGGACCCTTC TTCTCCGCGA
GCAAGCCCTG CCCACTCGCC CCGGGAAAAT GGAATCGACA AAAATCGCCT GCTAAAGAAG
GATGCTTCTA GCAGTCCAGC TTCCACGGCC TCCTCGGCAA GTTCCACTTC TTTGAAATCC
AAAGAAATGA GCTTGCATGA AAAAGCCAGC ACGCCTGTTC TGAAATCCAG CACACCAACG
CCTCGGAGCG ACATGCCAAC GCCGGGCACC AGCGCCACTC CAGGCCTCCG TCCAGGTCTC
GGCAAGCCTC CAGCCATAGA CCCCCTCGTT AACCAAGCGG CTGGCTTGAG GACACCCCTG
GCAGTGCCCG GCCCATATCC TGCTCCTTTT GGGATGGTCC CCCACGCTGG CATGAACGGC
GAGCTGACCA GCCCAGGCGC TGCCTACGCC AGTTTACACA ACATGTCGCC CCAGATGAGC
GCCGCAGCCG CCGCGGCCGC CGTGGTGGCC TACGGGCGCT CCCCCATGGT GGGGTTTGAT
CCTCCCCCTC ACATGAGAGT ACCTACCATT CCTCCAAACC TGGCAGGAAT CCCTGGGGGG
AAACCTGCAT ACTCCTTCCA CGTTACTGCA GACGGTCAGA TGCAGCCTGT CCCTTTTCCC
CCCGACGCCC TCATCGGACC CGGAATCCCC CGGCATGCTC GCCAGATCAA CACCCTCAAC
CACGGGGAGG TGGTGTGCGC TGTGACCATC AGCAACCCCA CGAGACACGT GTACACAGGC
GGGAAGGGCT GCGTCAAGGT CTGGGACATC AGCCACCCTG GCAATAAGAG CCCTGTCTCC
CAGCTCGACT GTCTGAACAG AGACAATTAT ATCCGTTCCT GTAAATTGCT ACCCGATGGC
TGCACTCTCA TAGTGGGAGG GGAAGCCAGT ACTTTGTCCA TTTGGGACCT GGCGGCTCCA
ACCCCGCGCA TCAAGGCGGA GCTGACGTCC TCGGCCCCCG CCTGCTACGC CCTGGCCATC
AGCCCCGATT CCAAGGTCTG CTTCTCATGC TGCAGCGACG GCAACATCGC TGTGTGGGAT
CTGCACAACC AGACACTAGT GAGGCAATTC CAGGGCCACA CAGACGGAGC CAGCTGTATT
GACATTTCTA ATGATGGCAC CAAGCTCTGG ACGGGTGGTT TGGACAACAC AGTCAGGTCC
TGGGACCTGC GCGAGGGGCG GCAGCTGCAG CAGCACGACT TCACCTCCCA GATCTTCTCC
CTGGGGTACT GCCCCACCGG GGAGTGGCTG GCAGTGGGCA TGGAGAGCAG CAATGTGGAG
GTGCTGCACG TGAACAAGCC TGACAAGTAC CAGCTGCACC TGCATGAGAG CTGCGTGCTG
TCCCTGAAAT TTGCTTACTG TGGTAAATGG TTTGTGAGTA CTGGAAAAGA TAACCTCCTC
AATGCTTGGC GGACCCCCTA TGGAGCCAGC ATATTCCAGT CCAAAGAGTC CTCGTCAGTG
CTTAGCTGTG ACATCTCTGT GGATGATAAG TACATAGTCA CTGGCTCGGG GGACAAGAAG
GCTACAGTCT ATGAAGTCAT CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717326.1
CDS210..2516
Misc Feature(1)210..605
Misc Feature(2)<1629..2507
Misc Feature(3)1650..2504
Misc Feature(4)order(1650..1652,1704..1706,1731..1736,1782..1787,1842..1844,1854..1856,1872..1877,1917..1922,1971..1973,1986..1988,2004..2009,2046..2048,2097..2099,2112..2114,2130..2135,2160..2165,2223..2225,2235..2237,2253..2258,2289..2294,2343..2345,2358..2360,2376..2381,2412..2417,2469..2471,2481..2483,2499..2504)
Misc Feature(5)1662..1781
Misc Feature(6)1803..1919
Misc Feature(7)1932..2042
Misc Feature(8)2061..2168
Misc Feature(9)2181..2282
Misc Feature(10)2304..2408
Misc Feature(11)2427..2501
Translation

Target ORF information:

RefSeq Version XM_006717263.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717263.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGTT GCAAGCTCAG CATCTTTCTC ATGGCCACGG ACCCCCAGTT
CCCCTTACGC CTCACCCTTC GGGACTTCAG CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT
GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG AGTGGGCAGT CTCACTTGGC AATAAAAGAT
GACAAGAAGC ACCACGATGC AGAGCACCAC AGAGACAGAG AGCCGGGCAC AAGTAATTCC
CTCCTGGTCC CAGACAGTCT AAGAGGCACA GATAAACGCA GAAATGGACC TGAATTTTCC
AATGACATCA AGAAAAGGAA GGTGGATGAT AAGGACTCCA GCCACTATGA CAGTGATGGT
GACAAAAGCG ATGACAACTT AGTTGTGGAT GTGTCTAATG AGGACCCTTC TTCTCCGCGA
GCAAGCCCTG CCCACTCGCC CCGGGAAAAT GGAATCGACA AAAATCGCCT GCTAAAGAAG
GATGCTTCTA GCAGTCCAGC TTCCACGGCC TCCTCGGCAA GTTCCACTTC TTTGAAATCC
AAAGAAATGA GCTTGCATGA AAAAGCCAGC ACGCCTGTTC TGAAATCCAG CACACCAACG
CCTCGGAGCG ACATGCCAAC GCCGGGCACC AGCGCCACTC CAGGCCTCCG TCCAGGTCTC
GGCAAGCCTC CAGCCATAGA CCCCCTCGTT AACCAAGCGG CTGGCTTGAG GACACCCCTG
GCAGTGCCCG GCCCATATCC TGCTCCTTTT GGGATGGTCC CCCACGCTGG CATGAACGGC
GAGCTGACCA GCCCAGGCGC TGCCTACGCC AGTTTACACA ACATGTCGCC CCAGATGAGC
GCCGCAGCCG CCGCGGCCGC CGTGGTGGCC TACGGGCGCT CCCCCATGGT GGGGTTTGAT
CCTCCCCCTC ACATGAGAGT ACCTACCATT CCTCCAAACC TGGCAGGAAT CCCTGGGGGG
AAACCTGCAT ACTCCTTCCA CGTTACTGCA GACGGTCAGA TGCAGCCTGT CCCTTTTCCC
CCCGACGCCC TCATCGGACC CGGAATCCCC CGGCATGCTC GCCAGATCAA CACCCTCAAC
CACGGGGAGG TGGTGTGCGC TGTGACCATC AGCAACCCCA CGAGACACGT GTACACAGGC
GGGAAGGGCT GCGTCAAGGT CTGGGACATC AGCCACCCTG GCAATAAGAG CCCTGTCTCC
CAGCTCGACT GTCTGAACAG AGACAATTAT ATCCGTTCCT GTAAATTGCT ACCCGATGGC
TGCACTCTCA TAGTGGGAGG GGAAGCCAGT ACTTTGTCCA TTTGGGACCT GGCGGCTCCA
ACCCCGCGCA TCAAGGCGGA GCTGACGTCC TCGGCCCCCG CCTGCTACGC CCTGGCCATC
AGCCCCGATT CCAAGGTCTG CTTCTCATGC TGCAGCGACG GCAACATCGC TGTGTGGGAT
CTGCACAACC AGACACTAGT GAGGCAATTC CAGGGCCACA CAGACGGAGC CAGCTGTATT
GACATTTCTA ATGATGGCAC CAAGCTCTGG ACGGGTGGTT TGGACAACAC AGTCAGGTCC
TGGGACCTGC GCGAGGGGCG GCAGCTGCAG CAGCACGACT TCACCTCCCA GATCTTCTCC
CTGGGGTACT GCCCCACCGG GGAGTGGCTG GCAGTGGGCA TGGAGAGCAG CAATGTGGAG
GTGCTGCACG TGAACAAGCC TGACAAGTAC CAGCTGCACC TGCATGAGAG CTGCGTGCTG
TCCCTGAAAT TTGCTTACTG TGGTAAATGG TTTGTGAGTA CTGGAAAAGA TAACCTCCTC
AATGCTTGGC GGACCCCCTA TGGAGCCAGC ATATTCCAGT CCAAAGAGTC CTCGTCAGTG
CTTAGCTGTG ACATCTCTGT GGATGATAAG TACATAGTCA CTGGCTCGGG GGACAAGAAG
GCTACAGTCT ATGAAGTCAT CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61005
Clone ID Related Accession (Same CDS sequence) XM_011518951.2
Accession Version XM_011518951.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGTT GCAAGCTCAG CATCTTTCTC ATGGCCACGG ACCCCCAGTT
CCCCTTACGC CTCACCCTTC GGGACTTCAG CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT
GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG AGTGGGCAGT CTCACTTGGC AATAAAAGAT
GACAAGAAGC ACCACGATGC AGAGCACCAC AGAGACAGAG AGCCGGGCAC AAGTAATTCC
CTCCTGGTCC CAGACAGTCT AAGAGGCACA GATAAACGCA GAAATGGACC TGAATTTTCC
AATGACATCA AGAAAAGGAA GGTGGATGAT AAGGACTCCA GCCACTATGA CAGTGATGGT
GACAAAAGCG ATGACAACTT AGTTGTGGAT GTGTCTAATG AGGACCCTTC TTCTCCGCGA
GCAAGCCCTG CCCACTCGCC CCGGGAAAAT GGAATCGACA AAAATCGCCT GCTAAAGAAG
GATGCTTCTA GCAGTCCAGC TTCCACGGCC TCCTCGGCAA GTTCCACTTC TTTGAAATCC
AAAGAAATGA GCTTGCATGA AAAAGCCAGC ACGCCTGTTC TGAAATCCAG CACACCAACG
CCTCGGAGCG ACATGCCAAC GCCGGGCACC AGCGCCACTC CAGGCCTCCG TCCAGGTCTC
GGCAAGCCTC CAGCCATAGA CCCCCTCGTT AACCAAGCGG CAGCTGGCTT GAGGACACCC
CTGGCAGTGC CCGGCCCATA TCCTGCTCCT TTTGGGATGG TCCCCCACGC TGGCATGAAC
GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC GCCAGTTTAC ACAACATGTC GCCCCAGATG
AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG GCCTACGGGC GCTCCCCCAT GGTGGGGTTT
GATCCTCCCC CTCACATGAG AGTACCTACC ATTCCTCCAA ACCTGGCAGG AATCCCTGGG
GGGAAACCTG CATACTCCTT CCACGTTACT GCAGACGGTC AGATGCAGCC TGTCCCTTTT
CCCCCCGACG CCCTCATCGG ACCCGGAATC CCCCGGCATG CTCGCCAGAT CAACACCCTC
AACCACGGGG AGGTGGTGTG CGCTGTGACC ATCAGCAACC CCACGAGACA CGTGTACACA
GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC ATCAGCCACC CTGGCAATAA GAGCCCTGTC
TCCCAGCTCG ACTGTCTGAA CAGAGACAAT TATATCCGTT CCTGTAAATT GCTACCCGAT
GGCTGCACTC TCATAGTGGG AGGGGAAGCC AGTACTTTGT CCATTTGGGA CCTGGCGGCT
CCAACCCCGC GCATCAAGGC GGAGCTGACG TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC
ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA TGCTGCAGCG ACGGCAACAT CGCTGTGTGG
GATCTGCACA ACCAGACACT AGTGAGGCAA TTCCAGGGCC ACACAGACGG AGCCAGCTGT
ATTGACATTT CTAATGATGG CACCAAGCTC TGGACGGGTG GTTTGGACAA CACAGTCAGG
TCCTGGGACC TGCGCGAGGG GCGGCAGCTG CAGCAGCACG ACTTCACCTC CCAGATCTTC
TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG CTGGCAGTGG GCATGGAGAG CAGCAATGTG
GAGGTGCTGC ACGTGAACAA GCCTGACAAG TACCAGCTGC ACCTGCATGA GAGCTGCGTG
CTGTCCCTGA AATTTGCTTA CTGTGGTAAA TGGTTTGTGA GTACTGGAAA AGATAACCTC
CTCAATGCTT GGCGGACCCC CTATGGAGCC AGCATATTCC AGTCCAAAGA GTCCTCGTCA
GTGCTTAGCT GTGACATCTC TGTGGATGAT AAGTACATAG TCACTGGCTC GGGGGACAAG
AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011517253.1
CDS210..2519
Misc Feature(1)210..605
Misc Feature(2)<1632..2510
Misc Feature(3)1653..2507
Misc Feature(4)order(1653..1655,1707..1709,1734..1739,1785..1790,1845..1847,1857..1859,1875..1880,1920..1925,1974..1976,1989..1991,2007..2012,2049..2051,2100..2102,2115..2117,2133..2138,2163..2168,2226..2228,2238..2240,2256..2261,2292..2297,2346..2348,2361..2363,2379..2384,2415..2420,2472..2474,2484..2486,2502..2507)
Misc Feature(5)1665..1784
Misc Feature(6)1806..1922
Misc Feature(7)1935..2045
Misc Feature(8)2064..2171
Misc Feature(9)2184..2285
Misc Feature(10)2307..2411
Misc Feature(11)2430..2504
Translation

Target ORF information:

RefSeq Version XM_011518951.2
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011518951.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGCAGCAGTT GCAAGCTCAG CATCTTTCTC ATGGCCACGG ACCCCCAGTT
CCCCTTACGC CTCACCCTTC GGGACTTCAG CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT
GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG AGTGGGCAGT CTCACTTGGC AATAAAAGAT
GACAAGAAGC ACCACGATGC AGAGCACCAC AGAGACAGAG AGCCGGGCAC AAGTAATTCC
CTCCTGGTCC CAGACAGTCT AAGAGGCACA GATAAACGCA GAAATGGACC TGAATTTTCC
AATGACATCA AGAAAAGGAA GGTGGATGAT AAGGACTCCA GCCACTATGA CAGTGATGGT
GACAAAAGCG ATGACAACTT AGTTGTGGAT GTGTCTAATG AGGACCCTTC TTCTCCGCGA
GCAAGCCCTG CCCACTCGCC CCGGGAAAAT GGAATCGACA AAAATCGCCT GCTAAAGAAG
GATGCTTCTA GCAGTCCAGC TTCCACGGCC TCCTCGGCAA GTTCCACTTC TTTGAAATCC
AAAGAAATGA GCTTGCATGA AAAAGCCAGC ACGCCTGTTC TGAAATCCAG CACACCAACG
CCTCGGAGCG ACATGCCAAC GCCGGGCACC AGCGCCACTC CAGGCCTCCG TCCAGGTCTC
GGCAAGCCTC CAGCCATAGA CCCCCTCGTT AACCAAGCGG CAGCTGGCTT GAGGACACCC
CTGGCAGTGC CCGGCCCATA TCCTGCTCCT TTTGGGATGG TCCCCCACGC TGGCATGAAC
GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC GCCAGTTTAC ACAACATGTC GCCCCAGATG
AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG GCCTACGGGC GCTCCCCCAT GGTGGGGTTT
GATCCTCCCC CTCACATGAG AGTACCTACC ATTCCTCCAA ACCTGGCAGG AATCCCTGGG
GGGAAACCTG CATACTCCTT CCACGTTACT GCAGACGGTC AGATGCAGCC TGTCCCTTTT
CCCCCCGACG CCCTCATCGG ACCCGGAATC CCCCGGCATG CTCGCCAGAT CAACACCCTC
AACCACGGGG AGGTGGTGTG CGCTGTGACC ATCAGCAACC CCACGAGACA CGTGTACACA
GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC ATCAGCCACC CTGGCAATAA GAGCCCTGTC
TCCCAGCTCG ACTGTCTGAA CAGAGACAAT TATATCCGTT CCTGTAAATT GCTACCCGAT
GGCTGCACTC TCATAGTGGG AGGGGAAGCC AGTACTTTGT CCATTTGGGA CCTGGCGGCT
CCAACCCCGC GCATCAAGGC GGAGCTGACG TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC
ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA TGCTGCAGCG ACGGCAACAT CGCTGTGTGG
GATCTGCACA ACCAGACACT AGTGAGGCAA TTCCAGGGCC ACACAGACGG AGCCAGCTGT
ATTGACATTT CTAATGATGG CACCAAGCTC TGGACGGGTG GTTTGGACAA CACAGTCAGG
TCCTGGGACC TGCGCGAGGG GCGGCAGCTG CAGCAGCACG ACTTCACCTC CCAGATCTTC
TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG CTGGCAGTGG GCATGGAGAG CAGCAATGTG
GAGGTGCTGC ACGTGAACAA GCCTGACAAG TACCAGCTGC ACCTGCATGA GAGCTGCGTG
CTGTCCCTGA AATTTGCTTA CTGTGGTAAA TGGTTTGTGA GTACTGGAAA AGATAACCTC
CTCAATGCTT GGCGGACCCC CTATGGAGCC AGCATATTCC AGTCCAAAGA GTCCTCGTCA
GTGCTTAGCT GTGACATCTC TGTGGATGAT AAGTACATAG TCACTGGCTC GGGGGACAAG
AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98859
Clone ID Related Accession (Same CDS sequence) XM_017015064.1
Accession Version XM_017015064.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGTT GCAAGCTCAG
CATCTTTCTC ATGGCCACGG ACCCCCAGTT CCCCTTACGC CTCACCCTTC GGGACTTCAG
CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG
AGTGGGCAGT CTCACTTGGC AATAAAAGAT GACAAGAAGC ACCACGATGC AGAGCACCAC
AGAGACAGAG AGCCGGGCAC AAGTAATTCC CTCCTGGTCC CAGACAGTCT AAGAGGCACA
GATAAACGCA GAAATGGACC TGAATTTTCC AATGACATCA AGAAAAGGAA GGTGGATGAT
AAGGACTCCA GCCACTATGA CAGTGATGGT GACAAAAGCG ATGACAACTT AGTTGTGGAT
GTGTCTAATG AGGACCCTTC TTCTCCGCGA GCAAGCCCTG CCCACTCGCC CCGGGAAAAT
GGAATCGACA AAAATCGCCT GCTAAAGAAG GATGCTTCTA GCAGTCCAGC TTCCACGGCC
TCCTCGGCAA GTTCCACTTC TTTGAAATCC AAAGAAATGA GCTTGCATGA AAAAGCCAGC
ACGCCTGTTC TGAAATCCAG CACACCAACG CCTCGGAGCG ACATGCCAAC GCCGGGCACC
AGCGCCACTC CAGGCCTCCG TCCAGGTCTC GGCAAGCCTC CAGCCATAGA CCCCCTCGTT
AACCAAGCGG CAGCTGGCTT GAGGACACCC CTGGCAGTGC CCGGCCCATA TCCTGCTCCT
TTTGGGATGG TCCCCCACGC TGGCATGAAC GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC
GCCAGTTTAC ACAACATGTC GCCCCAGATG AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG
GCCTACGGGC GCTCCCCCAT GGTGGGGTTT GATCCTCCCC CTCACATGAG AGTACCTACC
ATTCCTCCAA ACCTGGCAGG AATCCCTGGG GGGAAACCTG CATACTCCTT CCACGTTACT
GCAGACGGTC AGATGCAGCC TGTCCCTTTT CCCCCCGACG CCCTCATCGG ACCCGGAATC
CCCCGGCATG CTCGCCAGAT CAACACCCTC AACCACGGGG AGGTGGTGTG CGCTGTGACC
ATCAGCAACC CCACGAGACA CGTGTACACA GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC
ATCAGCCACC CTGGCAATAA GAGCCCTGTC TCCCAGCTCG ACTGTCTGAA CAGAGACAAT
TATATCCGTT CCTGTAAATT GCTACCCGAT GGCTGCACTC TCATAGTGGG AGGGGAAGCC
AGTACTTTGT CCATTTGGGA CCTGGCGGCT CCAACCCCGC GCATCAAGGC GGAGCTGACG
TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA
TGCTGCAGCG ACGGCAACAT CGCTGTGTGG GATCTGCACA ACCAGACACT AGTGAGGCAA
TTCCAGGGCC ACACAGACGG AGCCAGCTGT ATTGACATTT CTAATGATGG CACCAAGCTC
TGGACGGGTG GTTTGGACAA CACAGTCAGG TCCTGGGACC TGCGCGAGGG GCGGCAGCTG
CAGCAGCACG ACTTCACCTC CCAGATCTTC TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG
CTGGCAGTGG GCATGGAGAG CAGCAATGTG GAGGTGCTGC ACGTGAACAA GCCTGACAAG
TACCAGCTGC ACCTGCATGA GAGCTGCGTG CTGTCCCTGA AATTTGCTTA CTGTGGTAAA
TGGTTTGTGA GTACTGGAAA AGATAACCTC CTCAATGCTT GGCGGACCCC CTATGGAGCC
AGCATATTCC AGTCCAAAGA GTCCTCGTCA GTGCTTAGCT GTGACATCTC TGTGGATGAT
AAGTACATAG TCACTGGCTC GGGGGACAAG AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870553.1
CDS210..2549
Misc Feature(1)210..635
Misc Feature(2)<1662..2540
Misc Feature(3)1683..2537
Misc Feature(4)order(1683..1685,1737..1739,1764..1769,1815..1820,1875..1877,1887..1889,1905..1910,1950..1955,2004..2006,2019..2021,2037..2042,2079..2081,2130..2132,2145..2147,2163..2168,2193..2198,2256..2258,2268..2270,2286..2291,2322..2327,2376..2378,2391..2393,2409..2414,2445..2450,2502..2504,2514..2516,2532..2537)
Misc Feature(5)1695..1814
Misc Feature(6)1836..1952
Misc Feature(7)1965..2075
Misc Feature(8)2094..2201
Misc Feature(9)2214..2315
Misc Feature(10)2337..2441
Misc Feature(11)2460..2534
Translation

Target ORF information:

RefSeq Version XM_017015064.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015064.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGTT GCAAGCTCAG
CATCTTTCTC ATGGCCACGG ACCCCCAGTT CCCCTTACGC CTCACCCTTC GGGACTTCAG
CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG
AGTGGGCAGT CTCACTTGGC AATAAAAGAT GACAAGAAGC ACCACGATGC AGAGCACCAC
AGAGACAGAG AGCCGGGCAC AAGTAATTCC CTCCTGGTCC CAGACAGTCT AAGAGGCACA
GATAAACGCA GAAATGGACC TGAATTTTCC AATGACATCA AGAAAAGGAA GGTGGATGAT
AAGGACTCCA GCCACTATGA CAGTGATGGT GACAAAAGCG ATGACAACTT AGTTGTGGAT
GTGTCTAATG AGGACCCTTC TTCTCCGCGA GCAAGCCCTG CCCACTCGCC CCGGGAAAAT
GGAATCGACA AAAATCGCCT GCTAAAGAAG GATGCTTCTA GCAGTCCAGC TTCCACGGCC
TCCTCGGCAA GTTCCACTTC TTTGAAATCC AAAGAAATGA GCTTGCATGA AAAAGCCAGC
ACGCCTGTTC TGAAATCCAG CACACCAACG CCTCGGAGCG ACATGCCAAC GCCGGGCACC
AGCGCCACTC CAGGCCTCCG TCCAGGTCTC GGCAAGCCTC CAGCCATAGA CCCCCTCGTT
AACCAAGCGG CAGCTGGCTT GAGGACACCC CTGGCAGTGC CCGGCCCATA TCCTGCTCCT
TTTGGGATGG TCCCCCACGC TGGCATGAAC GGCGAGCTGA CCAGCCCAGG CGCTGCCTAC
GCCAGTTTAC ACAACATGTC GCCCCAGATG AGCGCCGCAG CCGCCGCGGC CGCCGTGGTG
GCCTACGGGC GCTCCCCCAT GGTGGGGTTT GATCCTCCCC CTCACATGAG AGTACCTACC
ATTCCTCCAA ACCTGGCAGG AATCCCTGGG GGGAAACCTG CATACTCCTT CCACGTTACT
GCAGACGGTC AGATGCAGCC TGTCCCTTTT CCCCCCGACG CCCTCATCGG ACCCGGAATC
CCCCGGCATG CTCGCCAGAT CAACACCCTC AACCACGGGG AGGTGGTGTG CGCTGTGACC
ATCAGCAACC CCACGAGACA CGTGTACACA GGCGGGAAGG GCTGCGTCAA GGTCTGGGAC
ATCAGCCACC CTGGCAATAA GAGCCCTGTC TCCCAGCTCG ACTGTCTGAA CAGAGACAAT
TATATCCGTT CCTGTAAATT GCTACCCGAT GGCTGCACTC TCATAGTGGG AGGGGAAGCC
AGTACTTTGT CCATTTGGGA CCTGGCGGCT CCAACCCCGC GCATCAAGGC GGAGCTGACG
TCCTCGGCCC CCGCCTGCTA CGCCCTGGCC ATCAGCCCCG ATTCCAAGGT CTGCTTCTCA
TGCTGCAGCG ACGGCAACAT CGCTGTGTGG GATCTGCACA ACCAGACACT AGTGAGGCAA
TTCCAGGGCC ACACAGACGG AGCCAGCTGT ATTGACATTT CTAATGATGG CACCAAGCTC
TGGACGGGTG GTTTGGACAA CACAGTCAGG TCCTGGGACC TGCGCGAGGG GCGGCAGCTG
CAGCAGCACG ACTTCACCTC CCAGATCTTC TCCCTGGGGT ACTGCCCCAC CGGGGAGTGG
CTGGCAGTGG GCATGGAGAG CAGCAATGTG GAGGTGCTGC ACGTGAACAA GCCTGACAAG
TACCAGCTGC ACCTGCATGA GAGCTGCGTG CTGTCCCTGA AATTTGCTTA CTGTGGTAAA
TGGTTTGTGA GTACTGGAAA AGATAACCTC CTCAATGCTT GGCGGACCCC CTATGGAGCC
AGCATATTCC AGTCCAAAGA GTCCTCGTCA GTGCTTAGCT GTGACATCTC TGTGGATGAT
AAGTACATAG TCACTGGCTC GGGGGACAAG AAGGCTACAG TCTATGAAGT CATCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98860
Clone ID Related Accession (Same CDS sequence) XM_017015065.1
Accession Version XM_017015065.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGTT GCAAGCTCAG
CATCTTTCTC ATGGCCACGG ACCCCCAGTT CCCCTTACGC CTCACCCTTC GGGACTTCAG
CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG
AGTGGGCAGT CTCACTTGGC AATAAAAGAT GACAAGAAGC ACCACGATGC AGAGCACCAC
AGAGACAGAG AGCCGGGCAC AAGTAATTCC CTCCTGGTCC CAGACAGTCT AAGAGGCACA
GATAAACGCA GAAATGGACC TGAATTTTCC AATGACATCA AGAAAAGGAA GGTGGATGAT
AAGGACTCCA GCCACTATGA CAGTGATGGT GACAAAAGCG ATGACAACTT AGTTGTGGAT
GTGTCTAATG AGGACCCTTC TTCTCCGCGA GCAAGCCCTG CCCACTCGCC CCGGGAAAAT
GGAATCGACA AAAATCGCCT GCTAAAGAAG GATGCTTCTA GCAGTCCAGC TTCCACGGCC
TCCTCGGCAA GTTCCACTTC TTTGAAATCC AAAGAAATGA GCTTGCATGA AAAAGCCAGC
ACGCCTGTTC TGAAATCCAG CACACCAACG CCTCGGAGCG ACATGCCAAC GCCGGGCACC
AGCGCCACTC CAGGCCTCCG TCCAGGTCTC GGCAAGCCTC CAGCCATAGA CCCCCTCGTT
AACCAAGCGG CTGGCTTGAG GACACCCCTG GCAGTGCCCG GCCCATATCC TGCTCCTTTT
GGGATGGTCC CCCACGCTGG CATGAACGGC GAGCTGACCA GCCCAGGCGC TGCCTACGCC
AGTTTACACA ACATGTCGCC CCAGATGAGC GCCGCAGCCG CCGCGGCCGC CGTGGTGGCC
TACGGGCGCT CCCCCATGGT GGGGTTTGAT CCTCCCCCTC ACATGAGAGT ACCTACCATT
CCTCCAAACC TGGCAGGAAT CCCTGGGGGG AAACCTGCAT ACTCCTTCCA CGTTACTGCA
GACGGTCAGA TGCAGCCTGT CCCTTTTCCC CCCGACGCCC TCATCGGACC CGGAATCCCC
CGGCATGCTC GCCAGATCAA CACCCTCAAC CACGGGGAGG TGGTGTGCGC TGTGACCATC
AGCAACCCCA CGAGACACGT GTACACAGGC GGGAAGGGCT GCGTCAAGGT CTGGGACATC
AGCCACCCTG GCAATAAGAG CCCTGTCTCC CAGCTCGACT GTCTGAACAG AGACAATTAT
ATCCGTTCCT GTAAATTGCT ACCCGATGGC TGCACTCTCA TAGTGGGAGG GGAAGCCAGT
ACTTTGTCCA TTTGGGACCT GGCGGCTCCA ACCCCGCGCA TCAAGGCGGA GCTGACGTCC
TCGGCCCCCG CCTGCTACGC CCTGGCCATC AGCCCCGATT CCAAGGTCTG CTTCTCATGC
TGCAGCGACG GCAACATCGC TGTGTGGGAT CTGCACAACC AGACACTAGT GAGGCAATTC
CAGGGCCACA CAGACGGAGC CAGCTGTATT GACATTTCTA ATGATGGCAC CAAGCTCTGG
ACGGGTGGTT TGGACAACAC AGTCAGGTCC TGGGACCTGC GCGAGGGGCG GCAGCTGCAG
CAGCACGACT TCACCTCCCA GATCTTCTCC CTGGGGTACT GCCCCACCGG GGAGTGGCTG
GCAGTGGGCA TGGAGAGCAG CAATGTGGAG GTGCTGCACG TGAACAAGCC TGACAAGTAC
CAGCTGCACC TGCATGAGAG CTGCGTGCTG TCCCTGAAAT TTGCTTACTG TGGTAAATGG
TTTGTGAGTA CTGGAAAAGA TAACCTCCTC AATGCTTGGC GGACCCCCTA TGGAGCCAGC
ATATTCCAGT CCAAAGAGTC CTCGTCAGTG CTTAGCTGTG ACATCTCTGT GGATGATAAG
TACATAGTCA CTGGCTCGGG GGACAAGAAG GCTACAGTCT ATGAAGTCAT CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870554.1
CDS210..2546
Translation

Target ORF information:

RefSeq Version XM_017015065.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015065.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGACTGAA
ATCGCCAAGA GATTGAATAC GATTTGTGCA CAAGTCATCC CATTTCTGTC TCAGGAACAT
CAACAACAGG TGGCCCAGGC TGTTGAACGT GCCAAACAGG TGACCATGGC AGAATTGAAT
GCCATCATCG GGGTACGTGG CCTACCAGGT CTACCTCCTA CACAGCAGTT GCAAGCTCAG
CATCTTTCTC ATGGCCACGG ACCCCCAGTT CCCCTTACGC CTCACCCTTC GGGACTTCAG
CCTCCTGGAA TCCCGCCCCT CGGGGGCAGT GCCGGCCTTC TTGCGCTGTC TAGTGCTCTG
AGTGGGCAGT CTCACTTGGC AATAAAAGAT GACAAGAAGC ACCACGATGC AGAGCACCAC
AGAGACAGAG AGCCGGGCAC AAGTAATTCC CTCCTGGTCC CAGACAGTCT AAGAGGCACA
GATAAACGCA GAAATGGACC TGAATTTTCC AATGACATCA AGAAAAGGAA GGTGGATGAT
AAGGACTCCA GCCACTATGA CAGTGATGGT GACAAAAGCG ATGACAACTT AGTTGTGGAT
GTGTCTAATG AGGACCCTTC TTCTCCGCGA GCAAGCCCTG CCCACTCGCC CCGGGAAAAT
GGAATCGACA AAAATCGCCT GCTAAAGAAG GATGCTTCTA GCAGTCCAGC TTCCACGGCC
TCCTCGGCAA GTTCCACTTC TTTGAAATCC AAAGAAATGA GCTTGCATGA AAAAGCCAGC
ACGCCTGTTC TGAAATCCAG CACACCAACG CCTCGGAGCG ACATGCCAAC GCCGGGCACC
AGCGCCACTC CAGGCCTCCG TCCAGGTCTC GGCAAGCCTC CAGCCATAGA CCCCCTCGTT
AACCAAGCGG CTGGCTTGAG GACACCCCTG GCAGTGCCCG GCCCATATCC TGCTCCTTTT
GGGATGGTCC CCCACGCTGG CATGAACGGC GAGCTGACCA GCCCAGGCGC TGCCTACGCC
AGTTTACACA ACATGTCGCC CCAGATGAGC GCCGCAGCCG CCGCGGCCGC CGTGGTGGCC
TACGGGCGCT CCCCCATGGT GGGGTTTGAT CCTCCCCCTC ACATGAGAGT ACCTACCATT
CCTCCAAACC TGGCAGGAAT CCCTGGGGGG AAACCTGCAT ACTCCTTCCA CGTTACTGCA
GACGGTCAGA TGCAGCCTGT CCCTTTTCCC CCCGACGCCC TCATCGGACC CGGAATCCCC
CGGCATGCTC GCCAGATCAA CACCCTCAAC CACGGGGAGG TGGTGTGCGC TGTGACCATC
AGCAACCCCA CGAGACACGT GTACACAGGC GGGAAGGGCT GCGTCAAGGT CTGGGACATC
AGCCACCCTG GCAATAAGAG CCCTGTCTCC CAGCTCGACT GTCTGAACAG AGACAATTAT
ATCCGTTCCT GTAAATTGCT ACCCGATGGC TGCACTCTCA TAGTGGGAGG GGAAGCCAGT
ACTTTGTCCA TTTGGGACCT GGCGGCTCCA ACCCCGCGCA TCAAGGCGGA GCTGACGTCC
TCGGCCCCCG CCTGCTACGC CCTGGCCATC AGCCCCGATT CCAAGGTCTG CTTCTCATGC
TGCAGCGACG GCAACATCGC TGTGTGGGAT CTGCACAACC AGACACTAGT GAGGCAATTC
CAGGGCCACA CAGACGGAGC CAGCTGTATT GACATTTCTA ATGATGGCAC CAAGCTCTGG
ACGGGTGGTT TGGACAACAC AGTCAGGTCC TGGGACCTGC GCGAGGGGCG GCAGCTGCAG
CAGCACGACT TCACCTCCCA GATCTTCTCC CTGGGGTACT GCCCCACCGG GGAGTGGCTG
GCAGTGGGCA TGGAGAGCAG CAATGTGGAG GTGCTGCACG TGAACAAGCC TGACAAGTAC
CAGCTGCACC TGCATGAGAG CTGCGTGCTG TCCCTGAAAT TTGCTTACTG TGGTAAATGG
TTTGTGAGTA CTGGAAAAGA TAACCTCCTC AATGCTTGGC GGACCCCCTA TGGAGCCAGC
ATATTCCAGT CCAAAGAGTC CTCGTCAGTG CTTAGCTGTG ACATCTCTGT GGATGATAAG
TACATAGTCA CTGGCTCGGG GGACAAGAAG GCTACAGTCT ATGAAGTCAT CTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98861
Clone ID Related Accession (Same CDS sequence) XM_017015066.1
Accession Version XM_017015066.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product transducin-like enhancer protein 1 isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGCAGCAG
CAGTTGCAAG CTCAGCATCT TTCTCATGGC CACGGACCCC CAGTTCCCCT TACGCCTCAC
CCTTCGGGAC TTCAGCCTCC TGGAATCCCG CCCCTCGGGG GCAGTGCCGG CCTTCTTGCG
CTGTCTAGTG CTCTGAGTGG GCAGTCTCAC TTGGCAATAA AAGATGACAA GAAGCACCAC
GATGCAGAGC ACCACAGAGA CAGAGAGCCG GGCACAAGTA ATTCCCTCCT GGTCCCAGAC
AGTCTAAGAG GCACAGATAA ACGCAGAAAT GGACCTGAAT TTTCCAATGA CATCAAGAAA
AGGAAGGTGG ATGATAAGGA CTCCAGCCAC TATGACAGTG ATGGTGACAA AAGCGATGAC
AACTTAGTTG TGGATGTGTC TAATGAGGAC CCTTCTTCTC CGCGAGCAAG CCCTGCCCAC
TCGCCCCGGG AAAATGGAAT CGACAAAAAT CGCCTGCTAA AGAAGGATGC TTCTAGCAGT
CCAGCTTCCA CGGCCTCCTC GGCAAGTTCC ACTTCTTTGA AATCCAAAGA AATGAGCTTG
CATGAAAAAG CCAGCACGCC TGTTCTGAAA TCCAGCACAC CAACGCCTCG GAGCGACATG
CCAACGCCGG GCACCAGCGC CACTCCAGGC CTCCGTCCAG GTCTCGGCAA GCCTCCAGCC
ATAGACCCCC TCGTTAACCA AGCGGCAGCT GGCTTGAGGA CACCCCTGGC AGTGCCCGGC
CCATATCCTG CTCCTTTTGG GATGGTCCCC CACGCTGGCA TGAACGGCGA GCTGACCAGC
CCAGGCGCTG CCTACGCCAG TTTACACAAC ATGTCGCCCC AGATGAGCGC CGCAGCCGCC
GCGGCCGCCG TGGTGGCCTA CGGGCGCTCC CCCATGGTGG GGTTTGATCC TCCCCCTCAC
ATGAGAGTAC CTACCATTCC TCCAAACCTG GCAGGAATCC CTGGGGGGAA ACCTGCATAC
TCCTTCCACG TTACTGCAGA CGGTCAGATG CAGCCTGTCC CTTTTCCCCC CGACGCCCTC
ATCGGACCCG GAATCCCCCG GCATGCTCGC CAGATCAACA CCCTCAACCA CGGGGAGGTG
GTGTGCGCTG TGACCATCAG CAACCCCACG AGACACGTGT ACACAGGCGG GAAGGGCTGC
GTCAAGGTCT GGGACATCAG CCACCCTGGC AATAAGAGCC CTGTCTCCCA GCTCGACTGT
CTGAACAGAG ACAATTATAT CCGTTCCTGT AAATTGCTAC CCGATGGCTG CACTCTCATA
GTGGGAGGGG AAGCCAGTAC TTTGTCCATT TGGGACCTGG CGGCTCCAAC CCCGCGCATC
AAGGCGGAGC TGACGTCCTC GGCCCCCGCC TGCTACGCCC TGGCCATCAG CCCCGATTCC
AAGGTCTGCT TCTCATGCTG CAGCGACGGC AACATCGCTG TGTGGGATCT GCACAACCAG
ACACTAGTGA GGCAATTCCA GGGCCACACA GACGGAGCCA GCTGTATTGA CATTTCTAAT
GATGGCACCA AGCTCTGGAC GGGTGGTTTG GACAACACAG TCAGGTCCTG GGACCTGCGC
GAGGGGCGGC AGCTGCAGCA GCACGACTTC ACCTCCCAGA TCTTCTCCCT GGGGTACTGC
CCCACCGGGG AGTGGCTGGC AGTGGGCATG GAGAGCAGCA ATGTGGAGGT GCTGCACGTG
AACAAGCCTG ACAAGTACCA GCTGCACCTG CATGAGAGCT GCGTGCTGTC CCTGAAATTT
GCTTACTGTG GTAAATGGTT TGTGAGTACT GGAAAAGATA ACCTCCTCAA TGCTTGGCGG
ACCCCCTATG GAGCCAGCAT ATTCCAGTCC AAAGAGTCCT CGTCAGTGCT TAGCTGTGAC
ATCTCTGTGG ATGATAAGTA CATAGTCACT GGCTCGGGGG ACAAGAAGGC TACAGTCTAT
GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870555.1
CDS118..2292
Misc Feature(1)118..>348
Misc Feature(2)<1405..2283
Misc Feature(3)1426..2280
Misc Feature(4)order(1426..1428,1480..1482,1507..1512,1558..1563,1618..1620,1630..1632,1648..1653,1693..1698,1747..1749,1762..1764,1780..1785,1822..1824,1873..1875,1888..1890,1906..1911,1942..1947,1999..2001,2011..2013,2029..2034,2065..2070,2119..2121,2134..2136,2152..2157,2188..2193,2245..2247,2257..2259,2275..2280)
Misc Feature(5)1438..1557
Misc Feature(6)1579..1695
Misc Feature(7)1708..1818
Misc Feature(8)1837..1944
Misc Feature(9)1957..2058
Misc Feature(10)2080..2184
Misc Feature(11)2203..2277
Translation

Target ORF information:

RefSeq Version XM_017015066.1
Organism Homo sapiens(Human)
Definition Homo sapiens transducin like enhancer of split 1 (TLE1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015066.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGTTCCCGC AGAGCCGGCA CCCGACGCCG CACCAGGCTG CAGGCCAGCC CTTCAAGTTC 
ACTATCCCGG AGTCCCTGGA CCGGATTAAA GAGGAATTCC AGTTCCTGCA GGCGCAGTAT
CACAGCCTTA AATTGGAATG TGAGAAACTG GCAAGTGAAA AGACAGAAAT GCAGAGGCAC
TATGTGATGT ATTATGAAAT GTCATATGGA TTAAACATTG AAATGCACAA ACAGCAGCAG
CAGTTGCAAG CTCAGCATCT TTCTCATGGC CACGGACCCC CAGTTCCCCT TACGCCTCAC
CCTTCGGGAC TTCAGCCTCC TGGAATCCCG CCCCTCGGGG GCAGTGCCGG CCTTCTTGCG
CTGTCTAGTG CTCTGAGTGG GCAGTCTCAC TTGGCAATAA AAGATGACAA GAAGCACCAC
GATGCAGAGC ACCACAGAGA CAGAGAGCCG GGCACAAGTA ATTCCCTCCT GGTCCCAGAC
AGTCTAAGAG GCACAGATAA ACGCAGAAAT GGACCTGAAT TTTCCAATGA CATCAAGAAA
AGGAAGGTGG ATGATAAGGA CTCCAGCCAC TATGACAGTG ATGGTGACAA AAGCGATGAC
AACTTAGTTG TGGATGTGTC TAATGAGGAC CCTTCTTCTC CGCGAGCAAG CCCTGCCCAC
TCGCCCCGGG AAAATGGAAT CGACAAAAAT CGCCTGCTAA AGAAGGATGC TTCTAGCAGT
CCAGCTTCCA CGGCCTCCTC GGCAAGTTCC ACTTCTTTGA AATCCAAAGA AATGAGCTTG
CATGAAAAAG CCAGCACGCC TGTTCTGAAA TCCAGCACAC CAACGCCTCG GAGCGACATG
CCAACGCCGG GCACCAGCGC CACTCCAGGC CTCCGTCCAG GTCTCGGCAA GCCTCCAGCC
ATAGACCCCC TCGTTAACCA AGCGGCAGCT GGCTTGAGGA CACCCCTGGC AGTGCCCGGC
CCATATCCTG CTCCTTTTGG GATGGTCCCC CACGCTGGCA TGAACGGCGA GCTGACCAGC
CCAGGCGCTG CCTACGCCAG TTTACACAAC ATGTCGCCCC AGATGAGCGC CGCAGCCGCC
GCGGCCGCCG TGGTGGCCTA CGGGCGCTCC CCCATGGTGG GGTTTGATCC TCCCCCTCAC
ATGAGAGTAC CTACCATTCC TCCAAACCTG GCAGGAATCC CTGGGGGGAA ACCTGCATAC
TCCTTCCACG TTACTGCAGA CGGTCAGATG CAGCCTGTCC CTTTTCCCCC CGACGCCCTC
ATCGGACCCG GAATCCCCCG GCATGCTCGC CAGATCAACA CCCTCAACCA CGGGGAGGTG
GTGTGCGCTG TGACCATCAG CAACCCCACG AGACACGTGT ACACAGGCGG GAAGGGCTGC
GTCAAGGTCT GGGACATCAG CCACCCTGGC AATAAGAGCC CTGTCTCCCA GCTCGACTGT
CTGAACAGAG ACAATTATAT CCGTTCCTGT AAATTGCTAC CCGATGGCTG CACTCTCATA
GTGGGAGGGG AAGCCAGTAC TTTGTCCATT TGGGACCTGG CGGCTCCAAC CCCGCGCATC
AAGGCGGAGC TGACGTCCTC GGCCCCCGCC TGCTACGCCC TGGCCATCAG CCCCGATTCC
AAGGTCTGCT TCTCATGCTG CAGCGACGGC AACATCGCTG TGTGGGATCT GCACAACCAG
ACACTAGTGA GGCAATTCCA GGGCCACACA GACGGAGCCA GCTGTATTGA CATTTCTAAT
GATGGCACCA AGCTCTGGAC GGGTGGTTTG GACAACACAG TCAGGTCCTG GGACCTGCGC
GAGGGGCGGC AGCTGCAGCA GCACGACTTC ACCTCCCAGA TCTTCTCCCT GGGGTACTGC
CCCACCGGGG AGTGGCTGGC AGTGGGCATG GAGAGCAGCA ATGTGGAGGT GCTGCACGTG
AACAAGCCTG ACAAGTACCA GCTGCACCTG CATGAGAGCT GCGTGCTGTC CCTGAAATTT
GCTTACTGTG GTAAATGGTT TGTGAGTACT GGAAAAGATA ACCTCCTCAA TGCTTGGCGG
ACCCCCTATG GAGCCAGCAT ATTCCAGTCC AAAGAGTCCT CGTCAGTGCT TAGCTGTGAC
ATCTCTGTGG ATGATAAGTA CATAGTCACT GGCTCGGGGG ACAAGAAGGC TACAGTCTAT
GAAGTCATCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Transducin-like enhancer of split-1 is expressed and functional in human macrophages.
FEBS letters590(1)43-52(2016 Jan)
De Paoli F,Copin C,Vanhoutte J,Derudas B,Vinod M,Zawadzki C,Susen S,Pattou F,Haulon S,Staels B,Eeckhoute J,Chinetti-Gbaguidi G


book

TLE1 promotes EMT in A549 lung cancer cells through suppression of E-cadherin.
Biochemical and biophysical research communications455(3-4)277-84(2014 Dec)
Yao X,Ireland SK,Pham T,Temple B,Chen R,Raj MH,Biliran H


book

Biological insights from 108 schizophrenia-associated genetic loci.
Nature511(7510)421-7(2014 Jul)


book

Grg3/TLE3 and Grg1/TLE1 induce monohormonal pancreatic β-cells while repressing α-cell functions.
Diabetes63(5)1804-16(2014 May)
Metzger DE,Liu C,Ziaie AS,Naji A,Zaret KS


book

Molecular functions of the TLE tetramerization domain in Wnt target gene repression.
The EMBO journal33(7)719-31(2014 Apr)
Chodaparambil JV,Pate KT,Hepler MR,Tsai BP,Muthurajan UM,Luger K,Waterman ML,Weis WI


 
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