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CASR cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CASR
Entrez Gene ID 846
Full Name calcium sensing receptor
Synonyms CAR,EIG8,FHH,FIH,GPRC2A,HHC,HHC1,HYPOC1,NSHPT,PCAR1,hCasR
General protein information
Preferred Names

calcium sensing receptor

Names

extracellular calcium-sensing receptor
parathyroid Ca(2+)-sensing receptor 1
parathyroid cell calcium-sensing receptor 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

3

3q13.33-q21.1

Summary The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq, Jul 2008].
Disorder MIM:

601199

Disorder Html: Hypocalciuric hypercalcemia, type I, 145980 (3); Hyperparathyroidism,

mRNA and Protein(s)

mRNA Protein Name
NM_000388.3 NP_000379.2 extracellular calcium-sensing receptor isoform 2 precursor
NM_001178065.1 NP_001171536.1 extracellular calcium-sensing receptor isoform 1 precursor
XM_017007324.1 XP_016862813.1 extracellular calcium-sensing receptor isoform X1
XM_005247837.2 XP_005247894.1 extracellular calcium-sensing receptor isoform X2
XM_006713789.3 XP_006713852.1 extracellular calcium-sensing receptor isoform X1
XM_017007325.1 XP_016862814.1 extracellular calcium-sensing receptor isoform X1



Macaca mulatta (rhesus monkey) CASR XP_001112009.2
Canis lupus familiaris (dog) CASR NP_001074978.1
Homo sapiens (human) CASR NP_001171536.1
Bos taurus (cattle) CASR NP_776427.1
Rattus norvegicus (Norway rat) Casr NP_058692.1
Danio rerio (zebrafish) LOC560607 XP_689097.1
Xenopus tropicalis (tropical clawed frog) LOC100496505 XP_004919842.1
Pan troglodytes (chimpanzee) CASR XP_516689.2
Mus musculus (house mouse) Casr NP_038831.2
Gallus gallus (chicken) CASR XP_416491.3


Related articles in PubMed

A Novel Mutation of the Calcium Sensing Receptor Gene in a Franconian Kindred: Heterozygous Mutation c.1697_1698delTG Exon 6.
Cordes M, Kuwert T, Haag C, Raue F
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme49(2)142-146(2017 Feb)

Heterozygous inactivating CaSR mutations causing neonatal hyperparathyroidism: function, inheritance and phenotype.
Glaudo M, Letz S, Quinkler M, Bogner U, Elbelt U, Strasburger CJ, Schnabel D, Lankes E, Scheel S, Feldkamp J, Haag C, Schulze E, Frank-Raue K, Raue F, Mayr B, Schöfl C
European journal of endocrinology175(5)421-31(2016 Nov)

Calcium sensing receptor effects in adipocytes and liver cells: Implications for an adipose-hepatic crosstalk.
Villarroel P, Mattar P, D'Espessailles A, Arrese M, Arreguin A, Fuentes C, Reyes M, Cifuentes M
Archives of biochemistry and biophysics60747-54(2016 Oct)

Structural mechanism of ligand activation in human calcium-sensing receptor.
Geng Y, Mosyak L, Kurinov I, Zuo H, Sturchler E, Cheng TC, Subramanyam P, Brown AP, Brennan SC, Mun HC, Bush M, Chen Y, Nguyen TX, Cao B, Chang DD, Quick M, Conigrave AD, Colecraft HM, McDonald P, Fan QR
eLife5(2016 Jul)

Calcium Oxalate Induces Renal Injury through Calcium-Sensing Receptor.
Li X, Ma J, Shi W, Su Y, Fu X, Yang Y, Lu J, Yue Z
Oxidative medicine and cellular longevity20165203801(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is a prospective observational study that measures the expression of vitamin D (VD) metabolising and signalling molecules and Ca(2+) sensing receptor (CaSR) in human Fallopian tube (FT) during the menstrual cycle and ectopic pregnancy (EP).
Title: Characterisation of vitamin D-related molecules and calcium-sensing receptor in human Fallopian tube during the menstrual cycle and in ectopic pregnancy.

CaSR and AP2S1 sequencing is worthwhile in patients with familial hyperparathyroidism and phenotype suggesting familial hypocalciuric hypercalcemia as it can diagnose up to 50% of cases.
Title: Stepwise CaSR, AP2S1, and GNA11 sequencing in patients with suspected familial hypocalciuric hypercalcemia.

The detection of CaSR gene mutations is suitable to differentiate states of hypercalcemia and may help to avoid invasive procedures such as parathyroidectomies.
Title: A Novel Mutation of the Calcium Sensing Receptor Gene in a Franconian Kindred: Heterozygous Mutation c.1697_1698delTG Exon 6.

CaSR expression was shown in HepG2 cells and human liver samples; CaSR may contribute to obesity-associated hepatic metabolic consequences
Title: Calcium sensing receptor effects in adipocytes and liver cells: Implications for an adipose-hepatic crosstalk.

reduced expression of the CaSR is correlated with activation of the renin-angiotensin system, which induces increased vascular remodeling and vascular smooth muscle cell proliferation, and thereby associated with essential hypertension in the SHR rat model and in the Han Chinese population
Title: Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.

The following CASR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27046 NM_000388.3
Latest version!
Homo sapiens calcium sensing receptor (CASR), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu38199 XM_005247837.2
Latest version!
Homo sapiens calcium sensing receptor (CASR), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu15961 NM_001178065.1
Latest version!
Homo sapiens calcium sensing receptor (CASR), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu38198 XM_006713789.3
Latest version!
Homo sapiens calcium sensing receptor (CASR), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OHu38198 XM_017007324.1
Latest version!
Homo sapiens calcium sensing receptor (CASR), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OHu38198 XM_017007325.1
Latest version!
Homo sapiens calcium sensing receptor (CASR), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27046
Clone ID Related Accession (Same CDS sequence) NM_000388.3
Accession Version NM_000388.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product extracellular calcium-sensing receptor isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068630.21, U20759.1, U20760.1, AC068754.16 and BP388878.1. This sequence is a reference standard in the RefSeqGene project. On Jun 5, 2008 this sequence version replaced NM_000388.2. Transcript Variant: This variant (2) has an alternate 5' UTR exon and lacks an in-frame segment in the CDS, as compared to variant 1. The resulting isoform (2) is shorter and lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U20759.1, D50855.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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2641
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2761
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3061
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ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCCTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC CAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000379.2
CDS373..3609
Misc Feature(1)334..336
Misc Feature(2)430..1962
Misc Feature(3)order(568..570,874..879,1618..1620)
Misc Feature(4)577..1857
Misc Feature(5)640..642
Misc Feature(6)order(685..687,694..696,835..837,1096..1098)
Misc Feature(7)760..762
Misc Feature(8)1153..1155
Misc Feature(9)1231..1233
Misc Feature(10)1708..1710
Misc Feature(11)1774..1776
Misc Feature(12)1834..1836
Misc Feature(13)1990..2142
Misc Feature(14)2209..2277
Misc Feature(15)2242..2949
Misc Feature(16)2320..2382
Misc Feature(17)2416..2472
Misc Feature(18)2545..2607
Misc Feature(19)2680..2748
Misc Feature(20)2788..2856
Misc Feature(21)2881..2958
Misc Feature(22)3010..3072
Misc Feature(23)3034..3036
Misc Feature(24)3034..3036
Misc Feature(25)3034..3036
Misc Feature(26)3130..3132
Misc Feature(27)3553..3555
Exon (1)1..130
Exon (2)131..557
Exon (3)558..864
Exon (4)865..1749
Exon (5)1750..1980
Exon (6)1981..2104
Exon (7)2105..4915
Translation

Target ORF information:

RefSeq Version NM_000388.3
Organism Homo sapiens(Human)
Definition Homo sapiens calcium sensing receptor (CASR), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000388.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCCTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC CAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38199
Clone ID Related Accession (Same CDS sequence) XM_005247837.2
Accession Version XM_005247837.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product extracellular calcium-sensing receptor isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005247837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTATCAGG TCAGTTATGC CTCCTCCAGC AGACTCCTCA GCAACAAGAA TCAATTCAAG 
TCTTTCCTCC GAACCATCCC CAATGATGAG CACCAGGCCA CTGCCATGGC AGACATCATC
GAGTATTTCC GCTGGAACTG GGTGGGCACA ATTGCAGCTG ATGACGACTA TGGGCGGCCG
GGGATTGAGA AATTCCGAGA GGAAGCTGAG GAAAGGGATA TCTGCATCGA CTTCAGTGAA
CTCATCTCCC AGTACTCTGA TGAGGAAGAG ATCCAGCATG TGGTAGAGGT GATTCAAAAT
TCCACGGCCA AAGTCATCGT GGTTTTCTCC AGTGGCCCAG ATCTTGAGCC CCTCATCAAG
GAGATTGTCC GGCGCAATAT CACGGGCAAG ATCTGGCTGG CCAGCGAGGC CTGGGCCAGC
TCCTCCCTGA TCGCCATGCC TCAGTACTTC CACGTGGTTG GCGGCACCAT TGGATTCGCT
CTGAAGGCTG GGCAGATCCC AGGCTTCCGG GAATTCCTGA AGAAGGTCCA TCCCAGGAAG
TCTGTCCACA ATGGTTTTGC CAAGGAGTTT TGGGAAGAAA CATTTAACTG CCACCTCCAA
GAAGGTGCAA AAGGACCTTT ACCTGTGGAC ACCTTTCTGA GAGGTCACGA AGAAAGTGGC
GACAGGTTTA GCAACAGCTC GACAGCCTTC CGACCCCTCT GTACAGGGGA TGAGAACATC
AGCAGTGTCG AGACCCCTTA CATAGATTAC ACGCATTTAC GGATATCCTA CAATGTGTAC
TTAGCAGTCT ACTCCATTGC CCACGCCTTG CAAGATATAT ATACCTGCTT ACCTGGGAGA
GGGCTCTTCA CCAATGGCTC CTGTGCAGAC ATCAAGAAAG TTGAGGCGTG GCAGGTCCTG
AAGCACCTAC GGCATCTAAA CTTTACAAAC AATATGGGGG AGCAGGTGAC CTTTGATGAG
TGTGGTGACC TGGTGGGGAA CTATTCCATC ATCAACTGGC ACCTCTCCCC AGAGGATGGC
TCCATCGTGT TTAAGGAAGT CGGGTATTAC AACGTCTATG CCAAGAAGGG AGAAAGACTC
TTCATCAACG AGGAGAAAAT CCTGTGGAGT GGGTTCTCCA GGGAGGTGCC CTTCTCCAAC
TGCAGCCGAG ACTGCCTGGC AGGGACCAGG AAAGGGATCA TTGAGGGGGA GCCCACCTGC
TGCTTTGAGT GTGTGGAGTG TCCTGATGGG GAGTATAGTG ATGAGACAGA TGCCAGTGCC
TGTAACAAGT GCCCAGATGA CTTCTGGTCC AATGAGAACC ACACCTCCTG CATTGCCAAG
GAGATCGAGT TTCTGTCGTG GACGGAGCCC TTTGGGATCG CACTCACCCT CTTTGCCGTG
CTGGGCATTT TCCTGACAGC CTTTGTGCTG GGTGTGTTTA TCAAGTTCCG CAACACACCC
ATTGTCAAGG CCACCAACCG AGAGCTCTCC TACCTCCTCC TCTTCTCCCT GCTCTGCTGC
TTCTCCAGCT CCCTGTTCTT CATCGGGGAG CCCCAGGACT GGACGTGCCG CCTGCGCCAG
CCGGCCTTTG GCATCAGCTT CGTGCTCTGC ATCTCATGCA TCCTGGTGAA AACCAACCGT
GTCCTCCTGG TGTTTGAGGC CAAGATCCCC ACCAGCTTCC ACCGCAAGTG GTGGGGGCTC
AACCTGCAGT TCCTGCTGGT TTTCCTCTGC ACCTTCATGC AGATTGTCAT CTGTGTGATC
TGGCTCTACA CCGCGCCCCC GTCAAGCTAC CGCAACCAGG AGCTGGAGGA TGAGATCATC
TTCATCACGT GCCACGAGGG CTCCCTCATG GCCCTGGGCT TCCTGATCGG CTACACCTGC
CTGCTGGCTG CCATCTGCTT CTTCTTTGCC TTCAAGTCCC GGAAGCTGCC GGAGAACTTC
AATGAAGCCA AGTTCATCAC CTTCAGCATG CTCATCTTCT TCATCGTCTG GATCTCCTTC
ATTCCAGCCT ATGCCAGCAC CTATGGCAAG TTTGTCTCTG CCGTAGAGGT GATTGCCATC
CTGGCAGCCA GCTTTGGCTT GCTGGCGTGC ATCTTCTTCA ACAAGATCTA CATCATTCTC
TTCAAGCCAT CCCGCAACAC CATCGAGGAG GTGCGTTGCA GCACCGCAGC TCACGCTTTC
AAGGTGGCTG CCCGGGCCAC GCTGCGCCGC AGCAACGTCT CCCGCAAGCG GTCCAGCAGC
CTTGGAGGCT CCACGGGATC CACCCCCTCC TCCTCCATCA GCAGCAAGAG CAACAGCGAA
GACCCATTCC CACAGCCCGA GAGGCAGAAG CAGCAGCAGC CGCTGGCCCT AACCCAGCAA
GAGCAGCAGC AGCAGCCCCT GACCCTCCCA CAGCAGCAAC GATCTCAGCA GCAGCCCAGA
TGCAAGCAGA AGGTCATCTT TGGCAGCGGC ACGGTCACCT TCTCACTGAG CTTTGATGAG
CCTCAGAAGA ACGCCATGGC CCACAGGAAT TCTACGCACC AGAACTCCCT GGAGGCCCAG
AAAAGCAGCG ATACGCTGAC CCGACACGAG CCATTACTCC CGCTGCAGTG CGGGGAAACG
GACTTAGATC TGACCGTCCA GGAAACAGGT CTGCAAGGAC CTGTGGGTGG AGACCAGCGG
CCAGAGGTGG AGGACCCTGA AGAGTTGTCC CCAGCACTTG TAGTGTCCAG TTCACAGAGC
TTTGTCATCA GTGGTGGAGG CAGCACTGTT ACAGAAAACG TAGTGAATTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247894.1
CDS86..2839
Misc Feature(1)<92..1192
Misc Feature(2)<92..1087
Misc Feature(3)1220..1372
Misc Feature(4)1472..2179
Translation

Target ORF information:

RefSeq Version XM_005247837.2
Organism Homo sapiens(Human)
Definition Homo sapiens calcium sensing receptor (CASR), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247837.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTATCAGG TCAGTTATGC CTCCTCCAGC AGACTCCTCA GCAACAAGAA TCAATTCAAG 
TCTTTCCTCC GAACCATCCC CAATGATGAG CACCAGGCCA CTGCCATGGC AGACATCATC
GAGTATTTCC GCTGGAACTG GGTGGGCACA ATTGCAGCTG ATGACGACTA TGGGCGGCCG
GGGATTGAGA AATTCCGAGA GGAAGCTGAG GAAAGGGATA TCTGCATCGA CTTCAGTGAA
CTCATCTCCC AGTACTCTGA TGAGGAAGAG ATCCAGCATG TGGTAGAGGT GATTCAAAAT
TCCACGGCCA AAGTCATCGT GGTTTTCTCC AGTGGCCCAG ATCTTGAGCC CCTCATCAAG
GAGATTGTCC GGCGCAATAT CACGGGCAAG ATCTGGCTGG CCAGCGAGGC CTGGGCCAGC
TCCTCCCTGA TCGCCATGCC TCAGTACTTC CACGTGGTTG GCGGCACCAT TGGATTCGCT
CTGAAGGCTG GGCAGATCCC AGGCTTCCGG GAATTCCTGA AGAAGGTCCA TCCCAGGAAG
TCTGTCCACA ATGGTTTTGC CAAGGAGTTT TGGGAAGAAA CATTTAACTG CCACCTCCAA
GAAGGTGCAA AAGGACCTTT ACCTGTGGAC ACCTTTCTGA GAGGTCACGA AGAAAGTGGC
GACAGGTTTA GCAACAGCTC GACAGCCTTC CGACCCCTCT GTACAGGGGA TGAGAACATC
AGCAGTGTCG AGACCCCTTA CATAGATTAC ACGCATTTAC GGATATCCTA CAATGTGTAC
TTAGCAGTCT ACTCCATTGC CCACGCCTTG CAAGATATAT ATACCTGCTT ACCTGGGAGA
GGGCTCTTCA CCAATGGCTC CTGTGCAGAC ATCAAGAAAG TTGAGGCGTG GCAGGTCCTG
AAGCACCTAC GGCATCTAAA CTTTACAAAC AATATGGGGG AGCAGGTGAC CTTTGATGAG
TGTGGTGACC TGGTGGGGAA CTATTCCATC ATCAACTGGC ACCTCTCCCC AGAGGATGGC
TCCATCGTGT TTAAGGAAGT CGGGTATTAC AACGTCTATG CCAAGAAGGG AGAAAGACTC
TTCATCAACG AGGAGAAAAT CCTGTGGAGT GGGTTCTCCA GGGAGGTGCC CTTCTCCAAC
TGCAGCCGAG ACTGCCTGGC AGGGACCAGG AAAGGGATCA TTGAGGGGGA GCCCACCTGC
TGCTTTGAGT GTGTGGAGTG TCCTGATGGG GAGTATAGTG ATGAGACAGA TGCCAGTGCC
TGTAACAAGT GCCCAGATGA CTTCTGGTCC AATGAGAACC ACACCTCCTG CATTGCCAAG
GAGATCGAGT TTCTGTCGTG GACGGAGCCC TTTGGGATCG CACTCACCCT CTTTGCCGTG
CTGGGCATTT TCCTGACAGC CTTTGTGCTG GGTGTGTTTA TCAAGTTCCG CAACACACCC
ATTGTCAAGG CCACCAACCG AGAGCTCTCC TACCTCCTCC TCTTCTCCCT GCTCTGCTGC
TTCTCCAGCT CCCTGTTCTT CATCGGGGAG CCCCAGGACT GGACGTGCCG CCTGCGCCAG
CCGGCCTTTG GCATCAGCTT CGTGCTCTGC ATCTCATGCA TCCTGGTGAA AACCAACCGT
GTCCTCCTGG TGTTTGAGGC CAAGATCCCC ACCAGCTTCC ACCGCAAGTG GTGGGGGCTC
AACCTGCAGT TCCTGCTGGT TTTCCTCTGC ACCTTCATGC AGATTGTCAT CTGTGTGATC
TGGCTCTACA CCGCGCCCCC GTCAAGCTAC CGCAACCAGG AGCTGGAGGA TGAGATCATC
TTCATCACGT GCCACGAGGG CTCCCTCATG GCCCTGGGCT TCCTGATCGG CTACACCTGC
CTGCTGGCTG CCATCTGCTT CTTCTTTGCC TTCAAGTCCC GGAAGCTGCC GGAGAACTTC
AATGAAGCCA AGTTCATCAC CTTCAGCATG CTCATCTTCT TCATCGTCTG GATCTCCTTC
ATTCCAGCCT ATGCCAGCAC CTATGGCAAG TTTGTCTCTG CCGTAGAGGT GATTGCCATC
CTGGCAGCCA GCTTTGGCTT GCTGGCGTGC ATCTTCTTCA ACAAGATCTA CATCATTCTC
TTCAAGCCAT CCCGCAACAC CATCGAGGAG GTGCGTTGCA GCACCGCAGC TCACGCTTTC
AAGGTGGCTG CCCGGGCCAC GCTGCGCCGC AGCAACGTCT CCCGCAAGCG GTCCAGCAGC
CTTGGAGGCT CCACGGGATC CACCCCCTCC TCCTCCATCA GCAGCAAGAG CAACAGCGAA
GACCCATTCC CACAGCCCGA GAGGCAGAAG CAGCAGCAGC CGCTGGCCCT AACCCAGCAA
GAGCAGCAGC AGCAGCCCCT GACCCTCCCA CAGCAGCAAC GATCTCAGCA GCAGCCCAGA
TGCAAGCAGA AGGTCATCTT TGGCAGCGGC ACGGTCACCT TCTCACTGAG CTTTGATGAG
CCTCAGAAGA ACGCCATGGC CCACAGGAAT TCTACGCACC AGAACTCCCT GGAGGCCCAG
AAAAGCAGCG ATACGCTGAC CCGACACGAG CCATTACTCC CGCTGCAGTG CGGGGAAACG
GACTTAGATC TGACCGTCCA GGAAACAGGT CTGCAAGGAC CTGTGGGTGG AGACCAGCGG
CCAGAGGTGG AGGACCCTGA AGAGTTGTCC CCAGCACTTG TAGTGTCCAG TTCACAGAGC
TTTGTCATCA GTGGTGGAGG CAGCACTGTT ACAGAAAACG TAGTGAATTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15961
Clone ID Related Accession (Same CDS sequence) NM_001178065.1
Accession Version NM_001178065.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product extracellular calcium-sensing receptor isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U20760.1, BC104999.1 and AC068754.16. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U20760.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1970526, SAMEA2145774 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGCC ACTCACCTTT
GTGCTGTCTG TCCTCCAGGT GCCCTTCTCC AACTGCAGCC GAGACTGCCT GGCAGGGACC
AGGAAAGGGA TCATTGAGGG GGAGCCCACC TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT
GGGGAGTATA GTGATGAGAC AGATGCCAGT GCCTGTAACA AGTGCCCAGA TGACTTCTGG
TCCAATGAGA ACCACACCTC CTGCATTGCC AAGGAGATCG AGTTTCTGTC GTGGACGGAG
CCCTTTGGGA TCGCACTCAC CCTCTTTGCC GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG
CTGGGTGTGT TTATCAAGTT CCGCAACACA CCCATTGTCA AGGCCACCAA CCGAGAGCTC
TCCTACCTCC TCCTCTTCTC CCTGCTCTGC TGCTTCTCCA GCTCCCTGTT CTTCATCGGG
GAGCCCCAGG ACTGGACGTG CCGCCTGCGC CAGCCGGCCT TTGGCATCAG CTTCGTGCTC
TGCATCTCAT GCATCCTGGT GAAAACCAAC CGTGTCCTCC TGGTGTTTGA GGCCAAGATC
CCCACCAGCT TCCACCGCAA GTGGTGGGGG CTCAACCTGC AGTTCCTGCT GGTTTTCCTC
TGCACCTTCA TGCAGATTGT CATCTGTGTG ATCTGGCTCT ACACCGCGCC CCCCTCAAGC
TACCGCAACC AGGAGCTGGA GGATGAGATC ATCTTCATCA CGTGCCACGA GGGCTCCCTC
ATGGCCCTGG GCTTCCTGAT CGGCTACACC TGCCTGCTGG CTGCCATCTG CTTCTTCTTT
GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC TTCAATGAAG CCAAGTTCAT CACCTTCAGC
ATGCTCATCT TCTTCATCGT CTGGATCTCC TTCATTCCAG CCTATGCCAG CACCTATGGC
AAGTTTGTCT CTGCCGTAGA GGTGATTGCC ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG
TGCATCTTCT TCAACAAGAT CTACATCATT CTCTTCAAGC CATCCCGCAA CACCATCGAG
GAGGTGCGTT GCAGCACCGC AGCTCACGCT TTCAAGGTGG CTGCCCGGGC CACGCTGCGC
CGCAGCAACG TCTCCCGCAA GCGGTCCAGC AGCCTTGGAG GCTCCACGGG ATCCACCCCC
TCCTCCTCCA TCAGCAGCAA GAGCAACAGC GAAGACCCAT TCCCACAGCC CGAGAGGCAG
AAGCAGCAGC AGCCGCTGGC CCTAACCCAG CAAGAGCAGC AGCAGCAGCC CCTGACCCTC
CCACAGCAGC AACGATCTCA GCAGCAGCCC AGATGCAAGC AGAAGGTCAT CTTTGGCAGC
GGCACGGTCA CCTTCTCACT GAGCTTTGAT GAGCCTCAGA AGAACGCCAT GGCCCACAGG
AATTCTACGC ACCAGAACTC CCTGGAGGCC CAGAAAAGCA GCGATACGCT GACCCGACAC
CAGCCATTAC TCCCGCTGCA GTGCGGGGAA ACGGACTTAG ATCTGACCGT CCAGGAAACA
GGTCTGCAAG GACCTGTGGG TGGAGACCAG CGGCCAGAGG TGGAGGACCC TGAAGAGTTG
TCCCCAGCAC TTGTAGTGTC CAGTTCACAG AGCTTTGTCA TCAGTGGTGG AGGCAGCACT
GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001171536.1
CDS439..3705
Misc Feature(1)400..402
Misc Feature(2)496..2028
Misc Feature(3)order(634..636,940..945,1684..1686)
Misc Feature(4)643..1923
Misc Feature(5)order(751..753,760..762,901..903,1162..1164)
Misc Feature(6)2086..2238
Misc Feature(7)2305..2373
Misc Feature(8)2338..3045
Misc Feature(9)2416..2478
Misc Feature(10)2512..2568
Misc Feature(11)2641..2703
Misc Feature(12)2776..2844
Misc Feature(13)2884..2952
Misc Feature(14)2977..3054
Misc Feature(15)3106..3168
Misc Feature(16)3226..3228
Misc Feature(17)3649..3651
Exon (1)1..196
Exon (2)197..623
Exon (3)624..930
Exon (4)931..1815
Exon (5)1816..2046
Exon (6)2047..2200
Exon (7)2201..5011
Translation

Target ORF information:

RefSeq Version NM_001178065.1
Organism Homo sapiens(Human)
Definition Homo sapiens calcium sensing receptor (CASR), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001178065.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGCC ACTCACCTTT
GTGCTGTCTG TCCTCCAGGT GCCCTTCTCC AACTGCAGCC GAGACTGCCT GGCAGGGACC
AGGAAAGGGA TCATTGAGGG GGAGCCCACC TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT
GGGGAGTATA GTGATGAGAC AGATGCCAGT GCCTGTAACA AGTGCCCAGA TGACTTCTGG
TCCAATGAGA ACCACACCTC CTGCATTGCC AAGGAGATCG AGTTTCTGTC GTGGACGGAG
CCCTTTGGGA TCGCACTCAC CCTCTTTGCC GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG
CTGGGTGTGT TTATCAAGTT CCGCAACACA CCCATTGTCA AGGCCACCAA CCGAGAGCTC
TCCTACCTCC TCCTCTTCTC CCTGCTCTGC TGCTTCTCCA GCTCCCTGTT CTTCATCGGG
GAGCCCCAGG ACTGGACGTG CCGCCTGCGC CAGCCGGCCT TTGGCATCAG CTTCGTGCTC
TGCATCTCAT GCATCCTGGT GAAAACCAAC CGTGTCCTCC TGGTGTTTGA GGCCAAGATC
CCCACCAGCT TCCACCGCAA GTGGTGGGGG CTCAACCTGC AGTTCCTGCT GGTTTTCCTC
TGCACCTTCA TGCAGATTGT CATCTGTGTG ATCTGGCTCT ACACCGCGCC CCCCTCAAGC
TACCGCAACC AGGAGCTGGA GGATGAGATC ATCTTCATCA CGTGCCACGA GGGCTCCCTC
ATGGCCCTGG GCTTCCTGAT CGGCTACACC TGCCTGCTGG CTGCCATCTG CTTCTTCTTT
GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC TTCAATGAAG CCAAGTTCAT CACCTTCAGC
ATGCTCATCT TCTTCATCGT CTGGATCTCC TTCATTCCAG CCTATGCCAG CACCTATGGC
AAGTTTGTCT CTGCCGTAGA GGTGATTGCC ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG
TGCATCTTCT TCAACAAGAT CTACATCATT CTCTTCAAGC CATCCCGCAA CACCATCGAG
GAGGTGCGTT GCAGCACCGC AGCTCACGCT TTCAAGGTGG CTGCCCGGGC CACGCTGCGC
CGCAGCAACG TCTCCCGCAA GCGGTCCAGC AGCCTTGGAG GCTCCACGGG ATCCACCCCC
TCCTCCTCCA TCAGCAGCAA GAGCAACAGC GAAGACCCAT TCCCACAGCC CGAGAGGCAG
AAGCAGCAGC AGCCGCTGGC CCTAACCCAG CAAGAGCAGC AGCAGCAGCC CCTGACCCTC
CCACAGCAGC AACGATCTCA GCAGCAGCCC AGATGCAAGC AGAAGGTCAT CTTTGGCAGC
GGCACGGTCA CCTTCTCACT GAGCTTTGAT GAGCCTCAGA AGAACGCCAT GGCCCACAGG
AATTCTACGC ACCAGAACTC CCTGGAGGCC CAGAAAAGCA GCGATACGCT GACCCGACAC
CAGCCATTAC TCCCGCTGCA GTGCGGGGAA ACGGACTTAG ATCTGACCGT CCAGGAAACA
GGTCTGCAAG GACCTGTGGG TGGAGACCAG CGGCCAGAGG TGGAGGACCC TGAAGAGTTG
TCCCCAGCAC TTGTAGTGTC CAGTTCACAG AGCTTTGTCA TCAGTGGTGG AGGCAGCACT
GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38198
Clone ID Related Accession (Same CDS sequence) XM_017007324.1 , XM_006713789.3 , XM_017007325.1
Accession Version XM_017007324.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product extracellular calcium-sensing receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016862813.1
CDS2319..5555
Misc Feature(1)2376..3908
Misc Feature(2)order(2514..2516,2820..2825,3564..3566)
Misc Feature(3)2523..3803
Misc Feature(4)order(2631..2633,2640..2642,2781..2783,3042..3044)
Misc Feature(5)3936..4088
Misc Feature(6)4188..4895
Translation

Target ORF information:

RefSeq Version XM_017007324.1
Organism Homo sapiens(Human)
Definition Homo sapiens calcium sensing receptor (CASR), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007324.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38198
Clone ID Related Accession (Same CDS sequence) XM_017007324.1 , XM_006713789.3 , XM_017007325.1
Accession Version XM_006713789.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product extracellular calcium-sensing receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713852.1
CDS954..4190
Misc Feature(1)1011..2543
Misc Feature(2)order(1149..1151,1455..1460,2199..2201)
Misc Feature(3)1158..2438
Misc Feature(4)order(1266..1268,1275..1277,1416..1418,1677..1679)
Misc Feature(5)2571..2723
Misc Feature(6)2823..3530
Translation

Target ORF information:

RefSeq Version XM_006713789.3
Organism Homo sapiens(Human)
Definition Homo sapiens calcium sensing receptor (CASR), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006713789.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu38198
Clone ID Related Accession (Same CDS sequence) XM_017007324.1 , XM_006713789.3 , XM_017007325.1
Accession Version XM_017007325.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product extracellular calcium-sensing receptor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016862814.1
CDS345..3581
Misc Feature(1)402..1934
Misc Feature(2)order(540..542,846..851,1590..1592)
Misc Feature(3)549..1829
Misc Feature(4)order(657..659,666..668,807..809,1068..1070)
Misc Feature(5)1962..2114
Misc Feature(6)2214..2921
Translation

Target ORF information:

RefSeq Version XM_017007325.1
Organism Homo sapiens(Human)
Definition Homo sapiens calcium sensing receptor (CASR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017007325.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGGCATTTT ATAGCTGCTG CTGGGTCCTC TTGGCACTCA CCTGGCACAC CTCTGCCTAC 
GGGCCAGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TTGGGGGGCT CTTTCCTATT
CATTTTGGAG TAGCAGCTAA AGATCAAGAT CTCAAATCAA GGCCGGAGTC TGTGGAATGT
ATCAGGTATA ATTTCCGTGG GTTTCGCTGG TTACAGGCTA TGATATTTGC CATAGAGGAG
ATAAACAGCA GCCCAGCCCT TCTTCCCAAC TTGACGCTGG GATACAGGAT ATTTGACACT
TGCAACACCG TTTCTAAGGC CTTGGAAGCC ACCCTGAGTT TTGTTGCTCA AAACAAAATT
GATTCTTTGA ACCTTGATGA GTTCTGCAAC TGCTCAGAGC ACATTCCCTC TACGATTGCT
GTGGTGGGAG CAACTGGCTC AGGCGTCTCC ACGGCAGTGG CAAATCTGCT GGGGCTCTTC
TACATTCCCC AGGTCAGTTA TGCCTCCTCC AGCAGACTCC TCAGCAACAA GAATCAATTC
AAGTCTTTCC TCCGAACCAT CCCCAATGAT GAGCACCAGG CCACTGCCAT GGCAGACATC
ATCGAGTATT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGGCGG
CCGGGGATTG AGAAATTCCG AGAGGAAGCT GAGGAAAGGG ATATCTGCAT CGACTTCAGT
GAACTCATCT CCCAGTACTC TGATGAGGAA GAGATCCAGC ATGTGGTAGA GGTGATTCAA
AATTCCACGG CCAAAGTCAT CGTGGTTTTC TCCAGTGGCC CAGATCTTGA GCCCCTCATC
AAGGAGATTG TCCGGCGCAA TATCACGGGC AAGATCTGGC TGGCCAGCGA GGCCTGGGCC
AGCTCCTCCC TGATCGCCAT GCCTCAGTAC TTCCACGTGG TTGGCGGCAC CATTGGATTC
GCTCTGAAGG CTGGGCAGAT CCCAGGCTTC CGGGAATTCC TGAAGAAGGT CCATCCCAGG
AAGTCTGTCC ACAATGGTTT TGCCAAGGAG TTTTGGGAAG AAACATTTAA CTGCCACCTC
CAAGAAGGTG CAAAAGGACC TTTACCTGTG GACACCTTTC TGAGAGGTCA CGAAGAAAGT
GGCGACAGGT TTAGCAACAG CTCGACAGCC TTCCGACCCC TCTGTACAGG GGATGAGAAC
ATCAGCAGTG TCGAGACCCC TTACATAGAT TACACGCATT TACGGATATC CTACAATGTG
TACTTAGCAG TCTACTCCAT TGCCCACGCC TTGCAAGATA TATATACCTG CTTACCTGGG
AGAGGGCTCT TCACCAATGG CTCCTGTGCA GACATCAAGA AAGTTGAGGC GTGGCAGGTC
CTGAAGCACC TACGGCATCT AAACTTTACA AACAATATGG GGGAGCAGGT GACCTTTGAT
GAGTGTGGTG ACCTGGTGGG GAACTATTCC ATCATCAACT GGCACCTCTC CCCAGAGGAT
GGCTCCATCG TGTTTAAGGA AGTCGGGTAT TACAACGTCT ATGCCAAGAA GGGAGAAAGA
CTCTTCATCA ACGAGGAGAA AATCCTGTGG AGTGGGTTCT CCAGGGAGGT GCCCTTCTCC
AACTGCAGCC GAGACTGCCT GGCAGGGACC AGGAAAGGGA TCATTGAGGG GGAGCCCACC
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGGGAGTATA GTGATGAGAC AGATGCCAGT
GCCTGTAACA AGTGCCCAGA TGACTTCTGG TCCAATGAGA ACCACACCTC CTGCATTGCC
AAGGAGATCG AGTTTCTGTC GTGGACGGAG CCCTTTGGGA TCGCACTCAC CCTCTTTGCC
GTGCTGGGCA TTTTCCTGAC AGCCTTTGTG CTGGGTGTGT TTATCAAGTT CCGCAACACA
CCCATTGTCA AGGCCACCAA CCGAGAGCTC TCCTACCTCC TCCTCTTCTC CCTGCTCTGC
TGCTTCTCCA GCTCCCTGTT CTTCATCGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGC
CAGCCGGCCT TTGGCATCAG CTTCGTGCTC TGCATCTCAT GCATCCTGGT GAAAACCAAC
CGTGTCCTCC TGGTGTTTGA GGCCAAGATC CCCACCAGCT TCCACCGCAA GTGGTGGGGG
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATTGT CATCTGTGTG
ATCTGGCTCT ACACCGCGCC CCCGTCAAGC TACCGCAACC AGGAGCTGGA GGATGAGATC
ATCTTCATCA CGTGCCACGA GGGCTCCCTC ATGGCCCTGG GCTTCCTGAT CGGCTACACC
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCCGGAAGCT GCCGGAGAAC
TTCAATGAAG CCAAGTTCAT CACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC
TTCATTCCAG CCTATGCCAG CACCTATGGC AAGTTTGTCT CTGCCGTAGA GGTGATTGCC
ATCCTGGCAG CCAGCTTTGG CTTGCTGGCG TGCATCTTCT TCAACAAGAT CTACATCATT
CTCTTCAAGC CATCCCGCAA CACCATCGAG GAGGTGCGTT GCAGCACCGC AGCTCACGCT
TTCAAGGTGG CTGCCCGGGC CACGCTGCGC CGCAGCAACG TCTCCCGCAA GCGGTCCAGC
AGCCTTGGAG GCTCCACGGG ATCCACCCCC TCCTCCTCCA TCAGCAGCAA GAGCAACAGC
GAAGACCCAT TCCCACAGCC CGAGAGGCAG AAGCAGCAGC AGCCGCTGGC CCTAACCCAG
CAAGAGCAGC AGCAGCAGCC CCTGACCCTC CCACAGCAGC AACGATCTCA GCAGCAGCCC
AGATGCAAGC AGAAGGTCAT CTTTGGCAGC GGCACGGTCA CCTTCTCACT GAGCTTTGAT
GAGCCTCAGA AGAACGCCAT GGCCCACAGG AATTCTACGC ACCAGAACTC CCTGGAGGCC
CAGAAAAGCA GCGATACGCT GACCCGACAC GAGCCATTAC TCCCGCTGCA GTGCGGGGAA
ACGGACTTAG ATCTGACCGT CCAGGAAACA GGTCTGCAAG GACCTGTGGG TGGAGACCAG
CGGCCAGAGG TGGAGGACCC TGAAGAGTTG TCCCCAGCAC TTGTAGTGTC CAGTTCACAG
AGCTTTGTCA TCAGTGGTGG AGGCAGCACT GTTACAGAAA ACGTAGTGAA TTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A Novel Mutation of the Calcium Sensing Receptor Gene in a Franconian Kindred: Heterozygous Mutation c.1697_1698delTG Exon 6.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme49(2)142-146(2017 Feb)
Cordes M,Kuwert T,Haag C,Raue F


book

Heterozygous inactivating CaSR mutations causing neonatal hyperparathyroidism: function, inheritance and phenotype.
European journal of endocrinology175(5)421-31(2016 Nov)
Glaudo M,Letz S,Quinkler M,Bogner U,Elbelt U,Strasburger CJ,Schnabel D,Lankes E,Scheel S,Feldkamp J,Haag C,Schulze E,Frank-Raue K,Raue F,Mayr B,Schöfl C


book

Calcium sensing receptor effects in adipocytes and liver cells: Implications for an adipose-hepatic crosstalk.
Archives of biochemistry and biophysics60747-54(2016 Oct)
Villarroel P,Mattar P,D'Espessailles A,Arrese M,Arreguin A,Fuentes C,Reyes M,Cifuentes M


book

Structural mechanism of ligand activation in human calcium-sensing receptor.
eLife5(2016 Jul)
Geng Y,Mosyak L,Kurinov I,Zuo H,Sturchler E,Cheng TC,Subramanyam P,Brown AP,Brennan SC,Mun HC,Bush M,Chen Y,Nguyen TX,Cao B,Chang DD,Quick M,Conigrave AD,Colecraft HM,McDonald P,Fan QR


book

Calcium Oxalate Induces Renal Injury through Calcium-Sensing Receptor.
Oxidative medicine and cellular longevity20165203801(2016)
Li X,Ma J,Shi W,Su Y,Fu X,Yang Y,Lu J,Yue Z


 
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