Casr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Casr
Entrez Gene ID	24247
Full Name	calcium-sensing receptor
Synonyms	PCaR1,RaKCaR
General protein information	Preferred Names calcium-sensing receptor Names extracellular calcium-sensing receptor Calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal hyperparathyroidism) parathyroid Cell calcium-sensing receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q22
Summary	a G protein-coupled receptor that senses extracellular calcium; involved in regulating the secretion of parathyroid hormone, bile and intestinal fluid [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597871.1	XP_017453360.1	extracellular calcium-sensing receptor isoform X1
XM_017597870.1	XP_017453359.1	extracellular calcium-sensing receptor isoform X1
NM_001309638.1	NP_001296567.1	extracellular calcium-sensing receptor precursor
XM_017597869.1	XP_017453358.1	extracellular calcium-sensing receptor isoform X1
XM_017597872.1	XP_017453361.1	extracellular calcium-sensing receptor isoform X1
NM_016996.2	NP_058692.1	extracellular calcium-sensing receptor precursor

Pathways from BioSystems

Homologies

	CASR	XP_001112009.2
Canis lupus familiaris (dog)	CASR	NP_001074978.1
Homo sapiens (human)	CASR	NP_001171536.1
Bos taurus (cattle)	CASR	NP_776427.1
Rattus norvegicus (Norway rat)	Casr	NP_058692.1
Danio rerio (zebrafish)	LOC560607	XP_689097.1
Xenopus tropicalis (tropical clawed frog)	LOC100496505	XP_004919842.1
Pan troglodytes (chimpanzee)	CASR	XP_516689.2
Mus musculus (house mouse)	Casr	NP_038831.2
Gallus gallus (chicken)	CASR	XP_416491.3

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of mitochondrial fission in calcium sensing receptor-mediated vascular smooth muscle cells proliferation during?hypertension.
Zhang X, Chen W, Li J, Qi S, Hong S, Wang Y, Gao L, Shi Z, Liu Y, Liu W, Chi Y, Liu C, Fu Y, Yin X
Biochemical and biophysical research communications495(1)454-460(2018 01)

Role of the calcium sensing receptor in cardiomyocyte apoptosis via mitochondrial dynamics in compensatory hypertrophied myocardium of spontaneously hypertensive rat.
Hong S, Zhang X, Zhang X, Liu W, Fu Y, Liu Y, Shi Z, Chi J, Zhao M, Yin X
Biochemical and biophysical research communications487(3)728-733(2017 06)

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.
Wang Y, Gao P, Wei C, Li H, Zhang L, Zhao Y, Wu B, Tian Y, Zhang W, Wu L, Wang R, Xu C
Cell death & disease8(5)e2799(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Calcium sensing receptor (CaSR) was increased in the aortas from spontaneous hypertensive rats (SHRs) compared with age-matched Wistar Kyoto (WKY) rats.
Title: Involvement of mitochondrial fission in calcium sensing receptor-mediated vascular smooth muscle cells proliferation during?hypertension.

These findings indicate that cardiac function could be enhanced significantly by combination therapy with CaSR inhibition and ESC transplantation; the effect was better than ESC transplantation alone, and the mechanism might be associated with a reduction in cell apoptosis via the inhibition of the MAPK pathway.
Title: Calcium-sensing receptor-mediated mitogen-activated protein kinase pathway improves the status of transplanted mouse embryonic stem cells in rats with acute myocardial infarction.

Our results demonstrated that CaSR activation induced cardiomyocyte apoptosis through the mitochondrial dynamics mediated apoptotic pathway in hypertensive hearts.
Title: Role of the calcium sensing receptor in cardiomyocyte apoptosis via mitochondrial dynamics in compensatory hypertrophied myocardium of spontaneously hypertensive rat.

The binding of HIMF enhanced the activity of extracellular CaSR and mediated hypoxia-evoked proliferation of pulmonary artery smooth cells and the development of pulmonary vascular remodeling and pulmonary hypertension
Title: Hypoxia-Induced Mitogenic Factor Acts as a Nonclassical Ligand of Calcium-Sensing Receptor, Therapeutically Exploitable for Intermittent Hypoxia-Induced Pulmonary Hypertension.

Monocrotaline aggregates on and activates the CaSR of pulmonary artery endothelial cells to trigger endothelial damage and, ultimately, induces pulmonary hypertension.
Title: Monocrotaline Induces Endothelial Injury and Pulmonary Hypertension by Targeting the Extracellular Calcium-Sensing Receptor.

The following Casr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Casr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa34304	NM_016996.2 Latest version!	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa34304	NM_001309638.1 Latest version!	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa34304	XM_017597869.1 Latest version!	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa34304	XM_017597872.1 Latest version!	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa34304	XM_017597870.1 Latest version!	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
ORa34304	XM_017597871.1 Latest version!	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Casr ( NM_001309638.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001296567.1 Rattus norvegicus calcium-sensing receptor (Casr), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa34304
Clone ID Related Accession (Same CDS sequence)	XM_017597871.1 , XM_017597870.1 , NM_001309638.1 , XM_017597869.1 , XM_017597872.1 , NM_016996.2
Accession Version	NM_001309638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	extracellular calcium-sensing receptor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07034351.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY214122.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04563757, SAMN04563758 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296567.1
CDS	573..3812
Translation	MASYSCCLALLALAWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPSLLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQYKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCQAGTRKGIIEGEPTCCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQILICIIWLYTAPPSSYRNHELEDEIIFITCHEGSLMALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRKRSSSLGGSTGSIPSSSISSKSNSEDRFPQPERQKQQQPLSLTQQEQQQQPLTLHPQQQQQPQQPRCKQKVIFGSGTVTFSLSFDEPQKNAMAHRNSMRQNSLEAQRSNDTLGRHQALLPLQCADADSEMTIQETGLQGPMVGDHQPEMESSDEMSPALVMSTSRSFVISGGGSSVTENVLHS

Target ORF information:

RefSeq Version	NM_001309638.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001309638.1

ORF Insert Sequence:

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casr ( NM_016996.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058692.1 Rattus norvegicus calcium-sensing receptor (Casr), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa34304
Clone ID Related Accession (Same CDS sequence)	XM_017597871.1 , XM_017597870.1 , NM_001309638.1 , XM_017597869.1 , XM_017597872.1 , NM_016996.2
Accession Version	NM_016996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	extracellular calcium-sensing receptor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07034351.1. On Jun 11, 2015 this sequence version replaced NM_016996.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642749 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058692.1
CDS	574..3813
Translation	MASYSCCLALLALAWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPSLLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQYKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCQAGTRKGIIEGEPTCCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQILICIIWLYTAPPSSYRNHELEDEIIFITCHEGSLMALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRKRSSSLGGSTGSIPSSSISSKSNSEDRFPQPERQKQQQPLSLTQQEQQQQPLTLHPQQQQQPQQPRCKQKVIFGSGTVTFSLSFDEPQKNAMAHRNSMRQNSLEAQRSNDTLGRHQALLPLQCADADSEMTIQETGLQGPMVGDHQPEMESSDEMSPALVMSTSRSFVISGGGSSVTENVLHS

Target ORF information:

RefSeq Version	NM_016996.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016996.2

ORF Insert Sequence:

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casr ( XM_017597871.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453360.1 Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X3, mRNA.

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CloneID	ORa34304
Clone ID Related Accession (Same CDS sequence)	XM_017597871.1 , XM_017597870.1 , NM_001309638.1 , XM_017597869.1 , XM_017597872.1 , NM_016996.2
Accession Version	XM_017597871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular calcium-sensing receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453360.1
CDS	483..3722
Translation	MASYSCCLALLALAWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPSLLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQYKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCQAGTRKGIIEGEPTCCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQILICIIWLYTAPPSSYRNHELEDEIIFITCHEGSLMALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRKRSSSLGGSTGSIPSSSISSKSNSEDRFPQPERQKQQQPLSLTQQEQQQQPLTLHPQQQQQPQQPRCKQKVIFGSGTVTFSLSFDEPQKNAMAHRNSMRQNSLEAQRSNDTLGRHQALLPLQCADADSEMTIQETGLQGPMVGDHQPEMESSDEMSPALVMSTSRSFVISGGGSSVTENVLHS

Target ORF information:

RefSeq Version	XM_017597871.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597871.1

ORF Insert Sequence:

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casr ( XM_017597870.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453359.1 Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X2, mRNA.

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CloneID	ORa34304
Clone ID Related Accession (Same CDS sequence)	XM_017597871.1 , XM_017597870.1 , NM_001309638.1 , XM_017597869.1 , XM_017597872.1 , NM_016996.2
Accession Version	XM_017597870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular calcium-sensing receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453359.1
CDS	562..3801
Translation	MASYSCCLALLALAWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPSLLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQYKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCQAGTRKGIIEGEPTCCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQILICIIWLYTAPPSSYRNHELEDEIIFITCHEGSLMALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRKRSSSLGGSTGSIPSSSISSKSNSEDRFPQPERQKQQQPLSLTQQEQQQQPLTLHPQQQQQPQQPRCKQKVIFGSGTVTFSLSFDEPQKNAMAHRNSMRQNSLEAQRSNDTLGRHQALLPLQCADADSEMTIQETGLQGPMVGDHQPEMESSDEMSPALVMSTSRSFVISGGGSSVTENVLHS

Target ORF information:

RefSeq Version	XM_017597870.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597870.1

ORF Insert Sequence:

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casr ( XM_017597869.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453358.1 Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X1, mRNA.

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CloneID	ORa34304
Clone ID Related Accession (Same CDS sequence)	XM_017597871.1 , XM_017597870.1 , NM_001309638.1 , XM_017597869.1 , XM_017597872.1 , NM_016996.2
Accession Version	XM_017597869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular calcium-sensing receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453358.1
CDS	3468..6707
Translation	MASYSCCLALLALAWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPSLLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQYKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCQAGTRKGIIEGEPTCCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQILICIIWLYTAPPSSYRNHELEDEIIFITCHEGSLMALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRKRSSSLGGSTGSIPSSSISSKSNSEDRFPQPERQKQQQPLSLTQQEQQQQPLTLHPQQQQQPQQPRCKQKVIFGSGTVTFSLSFDEPQKNAMAHRNSMRQNSLEAQRSNDTLGRHQALLPLQCADADSEMTIQETGLQGPMVGDHQPEMESSDEMSPALVMSTSRSFVISGGGSSVTENVLHS

Target ORF information:

RefSeq Version	XM_017597869.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597869.1

ORF Insert Sequence:

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casr ( XM_017597872.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453361.1 Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X4, mRNA.

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CloneID	ORa34304
Clone ID Related Accession (Same CDS sequence)	XM_017597871.1 , XM_017597870.1 , NM_001309638.1 , XM_017597869.1 , XM_017597872.1 , NM_016996.2
Accession Version	XM_017597872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	extracellular calcium-sensing receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453361.1
CDS	765..4004
Translation	MASYSCCLALLALAWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPSLLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQYKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCQAGTRKGIIEGEPTCCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQILICIIWLYTAPPSSYRNHELEDEIIFITCHEGSLMALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRKRSSSLGGSTGSIPSSSISSKSNSEDRFPQPERQKQQQPLSLTQQEQQQQPLTLHPQQQQQPQQPRCKQKVIFGSGTVTFSLSFDEPQKNAMAHRNSMRQNSLEAQRSNDTLGRHQALLPLQCADADSEMTIQETGLQGPMVGDHQPEMESSDEMSPALVMSTSRSFVISGGGSSVTENVLHS

Target ORF information:

RefSeq Version	XM_017597872.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium-sensing receptor (Casr), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597872.1

ORF Insert Sequence:

ATGGCATCGT ACAGCTGCTG TTTGGCCCTC TTGGCTCTTG CCTGGCACTC CTCTGCCTAT 
GGGCCTGACC AGCGAGCCCA AAAGAAGGGG GACATTATCC TAGGAGGTCT CTTTCCTATC 
CATTTTGGAG TAGCAGCCAA AGATCAAGAT CTGAAGTCAA GACCAGAGTC TGTGGAGTGC 
ATTAGGTATA ACTTCCGTGG ATTCCGATGG TTACAAGCCA TGATATTCGC CATAGAGGAG 
ATAAACAGCA GCCCCTCCCT TCTTCCCAAC ATGACACTGG GATATAGGAT ATTTGACACC 
TGTAACACCG TCTCCAAGGC GCTGGAAGCC ACCTTGAGTT TTGTTGCCCA GAACAAAATC 
GATTCTTTGA ACCTGGACGA GTTCTGCAAC TGCTCTGAGC ACATCCCTTC GACCATTGCC 
GTGGTGGGAG CCACCGGCTC CGGTGTCTCC ACGGCGGTAG CCAACCTGCT GGGACTTTTC 
TACATCCCCC AGGTGAGCTA CGCCTCCTCC AGCAGGCTCC TCAGCAATAA GAACCAGTAC 
AAATCCTTCC TCCGCACCAT TCCCAATGAC GAACACCAGG CAACCGCGAT GGCCGACATC 
ATCGAGTACT TCCGCTGGAA CTGGGTGGGC ACAATTGCAG CTGATGACGA CTATGGCAGA 
CCTGGCATTG AGAAGTTCCG AGAGGAAGCC GAAGAGAGGG ATATCTGCAT TGATTTTAGC 
GAGCTCATCT CCCAGTACTC TGACGAGGAA GAGATCCAGC AGGTGGTCGA AGTGATCCAA 
AACTCTACGG CCAAGGTCAT TGTCGTTTTC TCCAGCGGCC CGGACCTAGA ACCTCTCATC 
AAGGAGATTG TGCGGCGTAA CATCACAGGC AGGATCTGGC TGGCTAGCGA GGCCTGGGCC 
AGTTCCTCGC TGATTGCTAT GCCTGAGTAT TTCCATGTAG TCGGGGGCAC CATTGGGTTC 
GGTCTGAAGG CTGGGCAGAT TCCAGGCTTC CGAGAATTCC TACAGAAAGT TCATCCTAGG 
AAGTCTGTCC ACAATGGTTT TGCCAAAGAG TTTTGGGAAG AAACTTTTAA TTGCCACCTC 
CAAGAAGGCG CAAAAGGACC TTTACCTGTG GACACCTTCG TGAGAAGTCA CGAAGAAGGT 
GGCAACAGGT TACTCAATAG CTCTACTGCC TTCCGACCCC TCTGCACAGG GGATGAGAAC 
ATCAACAGTG TGGAGACCCC TTACATGGAT TACGAACATT TACGGATATC CTACAATGTG 
TACTTAGCCG TCTACTCCAT TGCGCATGCC CTACAAGATA TATACACCTG CTTACCCGGA 
AGAGGGCTTT TCACCAACGG GTCCTGTGCA GACATCAAGA AGGTTGAGGC CTGGCAGGTC 
TTGAAGCACC TACGGCACCT GAACTTCACC AACAACATGG GGGAGCAGGT GACCTTCGAT 
GAGTGTGGTG ATCTGGTGGG GAACTATTCT ATCATCAACT GGCACCTCTC CCCAGAGGAC 
GGCTCCATTG TGTTCAAGGA AGTTGGGTAC TACAATGTGT ATGCCAAGAA GGGAGAAAGA 
CTCTTCATCA ATGAGGAGAA GATCTTGTGG AGTGGGTTCT CCAGAGAGGT GCCTTTCTCC 
AATTGCAGCC GGGACTGTCA GGCAGGGACC AGGAAGGGGA TCATCGAGGG AGAGCCCACC 
TGCTGCTTTG AGTGTGTGGA GTGTCCTGAT GGAGAGTACA GTGGAGAGAC AGATGCGAGT 
GCCTGTGACA AGTGCCCGGA TGACTTCTGG TCCAATGAGA ACCACACTTC TTGCATTGCC 
AAGGAGATTG AGTTTCTGGC GTGGACCGAG CCCTTTGGAA TCGCTCTCAC TCTCTTTGCG 
GTGCTGGGCA TTTTCCTGAC CGCCTTTGTG CTGGGTGTCT TCATCAAGTT CCGAAACACA 
CCTATCGTCA AGGCCACCAA CCGAGAACTG TCCTACCTCC TGCTCTTCTC CCTACTCTGC 
TGCTTCTCCA GCTCCTTGTT CTTCATTGGG GAGCCCCAGG ACTGGACGTG CCGCCTGCGA 
CAGCCTGCTT TCGGCATCAG CTTTGTGCTC TGTATCTCGT GCATCTTGGT GAAGACCAAT 
CGCGTCCTCC TGGTATTTGA AGCCAAGATA CCCACCAGCT TCCACCGGAA GTGGTGGGGG 
CTCAACCTGC AGTTCCTGCT GGTTTTCCTC TGCACCTTCA TGCAGATCCT CATCTGCATC 
ATCTGGCTCT ACACGGCGCC CCCCTCTAGC TACCGCAACC ATGAGCTGGA AGACGAAATC 
ATCTTCATCA CGTGCCATGA GGGCTCACTC ATGGCACTTG GCTCCCTGAT CGGCTATACC 
TGCCTGCTGG CTGCCATCTG CTTCTTCTTT GCCTTCAAGT CCAGGAAGTT ACCAGAGAAC 
TTCAACGAAG CCAAGTTCAT TACCTTCAGC ATGCTCATCT TCTTCATCGT CTGGATCTCC 
TTCATTCCAG CCTATGCCAG CACCTACGGC AAGTTTGTCT CTGCCGTAGA GGTGATCGCC 
ATTTTGGCAG CCAGCTTTGG CTTACTAGCC TGCATCTTCT TCAACAAGGT CTACATTATC 
CTCTTCAAGC CTTCCCGGAA CACCATTGAG GAAGTCCGCT CCAGCACCGC AGCACATGCT 
TTCAAAGTAG CAGCCCGCGC CACTCTACGC CGTCCCAACA TCTCCCGGAA GCGGTCCAGC 
AGCCTTGGAG GCTCCACCGG CTCCATTCCC TCCTCCTCCA TCAGCAGCAA AAGCAACAGC 
GAAGACCGGT TCCCGCAGCC AGAGAGGCAG AAGCAACAGC AACCGCTGTC CCTGACCCAG 
CAAGAACAGC AGCAGCAGCC CCTGACCCTC CACCCACAGC AACAGCAGCA GCCACAGCAG 
CCGAGATGCA AACAGAAGGT CATCTTCGGC AGTGGTACGG TCACCTTCTC TCTGAGTTTT 
GACGAGCCTC AGAAGAATGC CATGGCCCAC AGGAACTCCA TGCGTCAGAA CTCCCTGGAG 
GCCCAGAGGA GCAACGACAC CTTGGGCAGA CACCAGGCCC TGCTTCCCCT ACAGTGTGCA 
GATGCGGACT CAGAAATGAC CATTCAGGAA ACGGGCCTGC AAGGGCCCAT GGTGGGGGAC 
CACCAGCCAG AAATGGAAAG CTCAGATGAA ATGTCCCCAG CGCTGGTCAT GTCCACCTCT 
CGGAGCTTCG TCATTAGTGG TGGAGGTAGC TCTGTGACGG AAAACGTATT ACACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of mitochondrial fission in calcium sensing receptor-mediated vascular smooth muscle cells proliferation during?hypertension.
Biochemical and biophysical research communications495(1)454-460(2018 01)
Zhang X,Chen W,Li J,Qi S,Hong S,Wang Y,Gao L,Shi Z,Liu Y,Liu W,Chi Y,Liu C,Fu Y,Yin X

Role of the calcium sensing receptor in cardiomyocyte apoptosis via mitochondrial dynamics in compensatory hypertrophied myocardium of spontaneously hypertensive rat.
Biochemical and biophysical research communications487(3)728-733(2017 06)
Hong S,Zhang X,Zhang X,Liu W,Fu Y,Liu Y,Shi Z,Chi J,Zhao M,Yin X

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.
Cell death & disease8(5)e2799(2017 May)
Wang Y,Gao P,Wei C,Li H,Zhang L,Zhao Y,Wu B,Tian Y,Zhang W,Wu L,Wang R,Xu C

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Casr cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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