PDE5A cDNA ORF clone, Homo sapiens(human)

The following PDE5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu00024 NM_033437.3
Latest version!
Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$414.50-$580.30
$829.00
OHu02694 NM_001083.4
Latest version!
Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu05453 NM_033430.3
Latest version!
Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu95522 XM_017008791.2
Latest version!
Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu95522 XM_017008791.1 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu05453 NM_033430.2 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu02694 NM_001083.3 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu00024
    Clone ID Related Accession (Same CDS sequence) NM_033437.3
    Accession Version NM_033437.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF155194.1, CD511199.1, BC126233.1, AC093752.2 and BM978332.1. On Jul 28, 2010 this sequence version replaced NM_033437.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (3, also known as PDE5A3) is shorter at the N-terminus, compared to isoform 1. There are no publicly available full-length transcripts supporting this variant; it is represented based on full-length cloning by RT-PCR in PMID:10679255. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.108416.1, SRR1803611.184255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCAATG CATGGTTTGC TGAGAGAGTT CACACCATCC CTGTGTGCAA GGAAGGTATC 
    AGAGGCCACA CCGAATCTTG CTCTTGTCCC TTGCAGCAGA GTCCTCGTGC AGATAACAGT
    GCCCCTGGAA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC
    ATTGTTGTCA AGGATTCTGA GGGAACTGTG AGCTTCCTCT CTGACTCAGA AAAGAAGGAA
    CAGATGCCTC TAACCCCTCC AAGGTTTGAT CATGATGAAG GGGACCAGTG CTCAAGACTC
    TTGGAATTAG TGAAGGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCACAAAATT
    TTCTTGCATA TCCATGGACT GATATCTGCT GACCGCTATT CCCTGTTCCT TGTCTGTGAA
    GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTCTTTG ATGTTGCTGA AGGTTCAACA
    CTGGAAGAAG TTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT
    GTGGCAGCGC TTGGTGAGCC CTTGAACATC AAAGATGCAT ATGAGGATCC TCGGTTCAAT
    GCAGAAGTTG ACCAAATTAC AGGCTACAAG ACACAAAGCA TTCTTTGTAT GCCAATTAAG
    AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAACGGT
    GGGACATTTA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT
    GTTCTTCATA ATGCTCAGCT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG
    CTGCTTGACC TTGCTAGTTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTTTGAAG
    AAAATAGCTG CCACTATTAT CTCTTTCATG CAAGTGCAGA AATGCACCAT TTTCATAGTG
    GATGAAGATT GCTCCGATTC TTTTTCTAGT GTGTTTCACA TGGAGTGTGA GGAATTAGAA
    AAATCATCTG ATACATTAAC AAGGGAACAT GATGCAAACA AAATCAATTA CATGTATGCT
    CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGATAAAAGA
    TTTCCCTGGA CAACTGAAAA TACAGGAAAT GTAAACCAGC AGTGCATTAG AAGTTTGCTT
    TGTACACCTA TAAAAAATGG AAAGAAGAAT AAAGTTATAG GGGTTTGCCA ACTTGTTAAT
    AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGACGA ACAGTTTCTG
    GAAGCTTTTG TCATCTTTTG TGGCTTGGGG ATCCAGAACA CGCAGATGTA TGAAGCAGTG
    GAGAGAGCCA TGGCCAAGCA AATGGTCACA TTGGAGGTTC TGTCGTATCA TGCTTCAGCA
    GCAGAGGAAG AAACAAGAGA GCTACAGTCG TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG
    ACCCTTAAAA TTACTGACTT TAGCTTCAGT GACTTTGAGC TGTCTGATCT GGAAACAGCA
    CTGTGTACAA TTCGGATGTT TACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT
    GAGGTTCTTT GCAGATGGAT TTTAAGTGTT AAGAAGAATT ATCGGAAGAA TGTTGCCTAT
    CATAATTGGA GACATGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTCT AAAAGCAGGC
    AAAATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCAT TGCTGATTGC TGCACTAAGC
    CACGATTTGG ATCACCGTGG TGTGAATAAC TCTTACATAC AGCGAAGTGA ACATCCACTT
    GCCCAGCTTT ACTGCCATTC AATCATGGAA CACCATCATT TTGACCAGTG CCTGATGATT
    CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG
    TTGAAAATAA TCAAGCAAGC TATTTTAGCT ACAGACCTAG CACTGTACAT TAAGAGGCGA
    GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAATT TGGAAGATCC TCATCAAAAG
    GAGTTGTTTT TGGCAATGCT GATGACAGCT TGTGATCTTT CTGCAATTAC AAAACCCTGG
    CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGATCA AGGAGACAGA
    GAGAGAAAAG AACTCAACAT AGAACCCACT GATCTAATGA ACAGGGAGAA GAAAAACAAA
    ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTGTA TGAGGCCCTG
    ACCCACGTGT CAGAGGACTG TTTCCCTTTG CTAGATGGCT GCAGAAAGAA CAGGCAGAAA
    TGGCAGGCCC TTGCAGAACA GCAGGAGAAG ATGCTGATTA ATGGGGAAAG CGGCCAGGCC
    AAGCGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_246273.2
    CDS95..2566
    Translation

    Target ORF information:

    RefSeq Version NM_033437.3
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGTCAATG CATGGTTTGC TGAGAGAGTT CACACCATCC CTGTGTGCAA GGAAGGTATC 
    AGAGGCCACA CCGAATCTTG CTCTTGTCCC TTGCAGCAGA GTCCTCGTGC AGATAACAGT
    GCCCCTGGAA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC
    ATTGTTGTCA AGGATTCTGA GGGAACTGTG AGCTTCCTCT CTGACTCAGA AAAGAAGGAA
    CAGATGCCTC TAACCCCTCC AAGGTTTGAT CATGATGAAG GGGACCAGTG CTCAAGACTC
    TTGGAATTAG TGAAGGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCACAAAATT
    TTCTTGCATA TCCATGGACT GATATCTGCT GACCGCTATT CCCTGTTCCT TGTCTGTGAA
    GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTCTTTG ATGTTGCTGA AGGTTCAACA
    CTGGAAGAAG TTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT
    GTGGCAGCGC TTGGTGAGCC CTTGAACATC AAAGATGCAT ATGAGGATCC TCGGTTCAAT
    GCAGAAGTTG ACCAAATTAC AGGCTACAAG ACACAAAGCA TTCTTTGTAT GCCAATTAAG
    AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAACGGT
    GGGACATTTA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT
    GTTCTTCATA ATGCTCAGCT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG
    CTGCTTGACC TTGCTAGTTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTTTGAAG
    AAAATAGCTG CCACTATTAT CTCTTTCATG CAAGTGCAGA AATGCACCAT TTTCATAGTG
    GATGAAGATT GCTCCGATTC TTTTTCTAGT GTGTTTCACA TGGAGTGTGA GGAATTAGAA
    AAATCATCTG ATACATTAAC AAGGGAACAT GATGCAAACA AAATCAATTA CATGTATGCT
    CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGATAAAAGA
    TTTCCCTGGA CAACTGAAAA TACAGGAAAT GTAAACCAGC AGTGCATTAG AAGTTTGCTT
    TGTACACCTA TAAAAAATGG AAAGAAGAAT AAAGTTATAG GGGTTTGCCA ACTTGTTAAT
    AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGACGA ACAGTTTCTG
    GAAGCTTTTG TCATCTTTTG TGGCTTGGGG ATCCAGAACA CGCAGATGTA TGAAGCAGTG
    GAGAGAGCCA TGGCCAAGCA AATGGTCACA TTGGAGGTTC TGTCGTATCA TGCTTCAGCA
    GCAGAGGAAG AAACAAGAGA GCTACAGTCG TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG
    ACCCTTAAAA TTACTGACTT TAGCTTCAGT GACTTTGAGC TGTCTGATCT GGAAACAGCA
    CTGTGTACAA TTCGGATGTT TACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT
    GAGGTTCTTT GCAGATGGAT TTTAAGTGTT AAGAAGAATT ATCGGAAGAA TGTTGCCTAT
    CATAATTGGA GACATGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTCT AAAAGCAGGC
    AAAATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCAT TGCTGATTGC TGCACTAAGC
    CACGATTTGG ATCACCGTGG TGTGAATAAC TCTTACATAC AGCGAAGTGA ACATCCACTT
    GCCCAGCTTT ACTGCCATTC AATCATGGAA CACCATCATT TTGACCAGTG CCTGATGATT
    CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG
    TTGAAAATAA TCAAGCAAGC TATTTTAGCT ACAGACCTAG CACTGTACAT TAAGAGGCGA
    GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAATT TGGAAGATCC TCATCAAAAG
    GAGTTGTTTT TGGCAATGCT GATGACAGCT TGTGATCTTT CTGCAATTAC AAAACCCTGG
    CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGATCA AGGAGACAGA
    GAGAGAAAAG AACTCAACAT AGAACCCACT GATCTAATGA ACAGGGAGAA GAAAAACAAA
    ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTGTA TGAGGCCCTG
    ACCCACGTGT CAGAGGACTG TTTCCCTTTG CTAGATGGCT GCAGAAAGAA CAGGCAGAAA
    TGGCAGGCCC TTGCAGAACA GCAGGAGAAG ATGCTGATTA ATGGGGAAAG CGGCCAGGCC
    AAGCGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02694
    Clone ID Related Accession (Same CDS sequence) NM_001083.3 , NM_001083.4
    Accession Version NM_001083.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Homo sapiens(human)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM920622.1, AF043731.1, AY264918.1, AC093752.2 and BM978332.1. On or before Mar 24, 2005 this sequence version replaced NM_033431.1, NM_001083.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as PDE5A1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126233.1, SRR1803611.275562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC
    CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG
    AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC
    ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT
    CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT
    TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA
    AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG
    ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA
    CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA
    CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC
    ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC
    CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG
    GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074.2
    CDS156..2783
    Translation

    Target ORF information:

    RefSeq Version NM_001083.3
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC
    CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG
    AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC
    ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT
    CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT
    TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA
    AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG
    ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA
    CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA
    CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC
    ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC
    CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG
    GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02694
    Clone ID Related Accession (Same CDS sequence) NM_001083.3 , NM_001083.4
    Accession Version NM_001083.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF043731.1, AY264918.1, AC093752.2 and BM978332.1. On Nov 23, 2018 this sequence version replaced NM_001083.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as PDE5A1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126233.1, SRR1803611.275562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000354960.8/ ENSP00000347046.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC
    CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG
    AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC
    ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT
    CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT
    TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA
    AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG
    ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA
    CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA
    CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC
    ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC
    CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG
    GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074.2
    CDS134..2761
    Translation

    Target ORF information:

    RefSeq Version NM_001083.4
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC
    CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG
    AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC
    ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT
    CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT
    TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA
    AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG
    ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA
    CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA
    CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC
    ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC
    CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG
    GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05453
    Clone ID Related Accession (Same CDS sequence) NM_033430.3 , NM_033430.2
    Accession Version NM_033430.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC080089.5, AF043732.1, AY264918.1, AC093752.2 and BM978332.1. On Jun 1, 2019 this sequence version replaced NM_033430.2. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2, also known as PDE5A2) has a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.247788.1, SRR1660805.155004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
    CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC
    TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT
    GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG
    AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT
    CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT
    TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT
    GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC
    TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC
    AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC
    CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC
    TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT
    TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA
    GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG
    CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT
    GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT
    GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT
    AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT
    GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT
    AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG
    CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG
    ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA
    TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG
    TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT
    AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT
    CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG
    ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC
    TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA
    CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT
    GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT
    ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT
    CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT
    TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA
    GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT
    GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG
    CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG
    ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_236914.2
    CDS563..3064
    Translation

    Target ORF information:

    RefSeq Version NM_033430.3
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033430.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
    CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC
    TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT
    GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG
    AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT
    CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT
    TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT
    GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC
    TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC
    AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC
    CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC
    TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT
    TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA
    GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG
    CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT
    GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT
    GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT
    AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT
    GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT
    AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG
    CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG
    ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA
    TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG
    TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT
    AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT
    CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG
    ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC
    TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA
    CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT
    GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT
    ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT
    CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT
    TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA
    GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT
    GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG
    CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG
    ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05453
    Clone ID Related Accession (Same CDS sequence) NM_033430.3 , NM_033430.2
    Accession Version NM_033430.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-22
    Organism Homo sapiens(human)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF043732.1, AY264918.1, AC093752.2 and BM978332.1. On Mar 24, 2005 this sequence version replaced NM_033430.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2, also known as PDE5A2) has a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.247788.1, SRR1660805.155004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
    CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC
    TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT
    GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG
    AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT
    CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT
    TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT
    GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC
    TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC
    AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC
    CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC
    TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT
    TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA
    GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG
    CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT
    GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT
    GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT
    AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT
    GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT
    AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG
    CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG
    ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA
    TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG
    TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT
    AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT
    CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG
    ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC
    TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA
    CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT
    GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT
    ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT
    CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT
    TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA
    GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT
    GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG
    CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG
    ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_236914.2
    CDS113..2614
    Translation

    Target ORF information:

    RefSeq Version NM_033430.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033430.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
    CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC
    TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT
    GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG
    AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT
    CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT
    TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT
    GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC
    CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC
    TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC
    AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG
    ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC
    CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC
    TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT
    TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA
    GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG
    CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT
    GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT
    GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT
    AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT
    GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT
    AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG
    CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG
    ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA
    TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG
    TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT
    GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC
    AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT
    AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT
    CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG
    ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC
    TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA
    CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT
    GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT
    ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT
    CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT
    TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA
    GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT
    GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT
    GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG
    CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG
    ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95522
    Clone ID Related Accession (Same CDS sequence) XM_017008791.2 , XM_017008791.1
    Accession Version XM_017008791.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG
    GGAATCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864280.1
    CDS37..1968
    Translation

    Target ORF information:

    RefSeq Version XM_017008791.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008791.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG
    GGAATCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95522
    Clone ID Related Accession (Same CDS sequence) XM_017008791.2 , XM_017008791.1
    Accession Version XM_017008791.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG
    GGAATCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864280.1
    CDS410..2341
    Translation

    Target ORF information:

    RefSeq Version XM_017008791.1
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
    AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT
    ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG
    AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT
    TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA
    ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA
    ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG
    TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT
    AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA
    TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT
    ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT
    ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG
    AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC
    TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT
    GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA
    AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT
    GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT
    TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT
    TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT
    TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG
    GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA
    CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA
    GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG
    AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG
    GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC
    TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG
    GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA
    CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC
    TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT
    GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA
    AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT
    ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG
    GGAATCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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