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PDE5A cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol PDE5A
Entrez Gene ID 8654
Full Name phosphodiesterase 5A
Synonyms CGB-PDE,CN5A,PDE5
General protein information
Preferred Names

phosphodiesterase 5A

Names

cGMP-specific 3',5'-cyclic phosphodiesterase
cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
cGMP-specific phosphodiesterase PDE5A2
cGMP-specific phosphodiesterase type 5A
phosphodiesterase 5A, cGMP-specific
phosphodiesterase isozyme 5
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

4

4q26
Summary This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:

603310


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_033430.3 NP_236914.2 cGMP-specific 3',5'-cyclic phosphodiesterase isoform 2
XM_017008791.2 XP_016864280.1 cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
NM_001083.3 NP_001074.2 cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
NM_033437.3 NP_246273.2 cGMP-specific 3',5'-cyclic phosphodiesterase isoform 3
XM_017008791.1 XP_016864280.1 cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
NM_033430.2 NP_236914.2 cGMP-specific 3',5'-cyclic phosphodiesterase isoform 2
NM_001083.4 NP_001074.2 cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1

Pathways from BioSystems


Homologies


Danio rerio (zebrafish) pde5ab NP_001116732.1
Drosophila melanogaster (fruit fly) Pde11 NP_001188837.1
Pan troglodytes (chimpanzee) PDE5A XP_001150967.2
PDE5A XP_001099652.1
Canis lupus familiaris (dog) PDE5A NP_001003188.1
Bos taurus (cattle) PDE5A NP_776842.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP008646 XP_314743.3
Rattus norvegicus (Norway rat) Pde5a NP_598268.1
Xenopus tropicalis (tropical clawed frog) pde5a NP_001072812.1
Homo sapiens (human) PDE5A NP_001074.2
Mus musculus (house mouse) Pde5a NP_700471.2
Gallus gallus (chicken) PDE5A XP_420631.3

Gene Ontology


Bibliography

Related articles in PubMed

Distinct phosphodiesterase 5A-containing compartments allow selective regulation of cGMP-dependent signalling in human arterial smooth muscle cells.
Wilson LS, Guo M, Umana MB, Maurice DH
Cellular signalling36204-211(2017 Aug)

Inhibition of PDE5A1 guanosine cyclic monophosphate (cGMP) hydrolysing activity by sildenafil analogues that inhibit cellular cGMP efflux.
Subbotina A, Ravna AW, Lysaa RA, Abagyan R, Bugno R, Sager G
The Journal of pharmacy and pharmacology69(6)675-683(2017 Jun)

Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome.
Cesarini V, Martini M, Vitiani LR, Gravina GL, Di Agostino S, Graziani G, D'Alessandris QG, Pallini R, Larocca LM, Rossi P, Jannini EA, Dolci S
Oncotarget8(8)13223-13239(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that high type 5 phosphodiesterase (PDE5) expression in glioblastoma multiforme (GBM) cells significantly correlated with longer overall survival of patients.
Title: Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome.

the relationship between PDE5A polymorphisms, diabetes, and the efficacy of sildenafil treatment.The response to sildenafil treatment depends on polymorphisms in the PDE5A gene and the glycemic status of the patients.
Title: PDE5A Polymorphisms Influence on Sildenafil Treatment Success.

Study showed that past attempts to quantify PDE5 mRNA were flawed due to the use of incorrect primers, and that when correct primers are used, PDE5 mRNA is detectable in human brain tissue; that PDE5 protein exists in human brain by western blot and ELISA; and performed immunohistochemistry and demonstrate that PDE5 is present in human neurons.
Title: PDE5 Exists in Human Neurons and is a Viable Therapeutic Target for Neurologic Disease.

Our data showed a significant and previously undocumented upregulation of PDE5 in both rat and human BPH.
Title: Upregulation of Phosphodiesterase type 5 in the Hyperplastic Prostate.

cGMP PDE isozymes, PDE5 and 10, are elevated in colon tumor cells compared with normal colonocytes, and inhibitors and siRNAs can selectively suppress colon tumor cell growth
Title: Suppression of ?-catenin/TCF transcriptional activity and colon tumor cell growth by dual inhibition of PDE5 and 10.

The following PDE5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu00024 NM_033437.3
Latest version!		 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $414.50-$580.30
$829.00
OHu02694 NM_001083.4
Latest version!		 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu05453 NM_033430.3
Latest version!		 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $587.30
$839.00
OHu95522 XM_017008791.2
Latest version!		 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu95522 XM_017008791.1 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu05453 NM_033430.2 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $587.30
$839.00
OHu02694 NM_001083.3 Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu00024
Clone ID Related Accession (Same CDS sequence) NM_033437.3
Accession Version NM_033437.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-03
Organism Homo sapiens(human)
Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF155194.1, CD511199.1, BC126233.1, AC093752.2 and BM978332.1. On Jul 28, 2010 this sequence version replaced NM_033437.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (3, also known as PDE5A3) is shorter at the N-terminus, compared to isoform 1. There are no publicly available full-length transcripts supporting this variant; it is represented based on full-length cloning by RT-PCR in PMID:10679255. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.108416.1, SRR1803611.184255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
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241
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361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGTCAATG CATGGTTTGC TGAGAGAGTT CACACCATCC CTGTGTGCAA GGAAGGTATC 
AGAGGCCACA CCGAATCTTG CTCTTGTCCC TTGCAGCAGA GTCCTCGTGC AGATAACAGT 
GCCCCTGGAA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTTGTCA AGGATTCTGA GGGAACTGTG AGCTTCCTCT CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCTCC AAGGTTTGAT CATGATGAAG GGGACCAGTG CTCAAGACTC 
TTGGAATTAG TGAAGGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCACAAAATT 
TTCTTGCATA TCCATGGACT GATATCTGCT GACCGCTATT CCCTGTTCCT TGTCTGTGAA 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTCTTTG ATGTTGCTGA AGGTTCAACA 
CTGGAAGAAG TTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCGC TTGGTGAGCC CTTGAACATC AAAGATGCAT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGCTACAAG ACACAAAGCA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAACGGT 
GGGACATTTA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAGCT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGTTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTTTGAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAAGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGT GTGTTTCACA TGGAGTGTGA GGAATTAGAA 
AAATCATCTG ATACATTAAC AAGGGAACAT GATGCAAACA AAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGATAAAAGA 
TTTCCCTGGA CAACTGAAAA TACAGGAAAT GTAAACCAGC AGTGCATTAG AAGTTTGCTT 
TGTACACCTA TAAAAAATGG AAAGAAGAAT AAAGTTATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGACGA ACAGTTTCTG 
GAAGCTTTTG TCATCTTTTG TGGCTTGGGG ATCCAGAACA CGCAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA AATGGTCACA TTGGAGGTTC TGTCGTATCA TGCTTCAGCA 
GCAGAGGAAG AAACAAGAGA GCTACAGTCG TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG 
ACCCTTAAAA TTACTGACTT TAGCTTCAGT GACTTTGAGC TGTCTGATCT GGAAACAGCA 
CTGTGTACAA TTCGGATGTT TACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGATGGAT TTTAAGTGTT AAGAAGAATT ATCGGAAGAA TGTTGCCTAT 
CATAATTGGA GACATGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTCT AAAAGCAGGC 
AAAATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCAT TGCTGATTGC TGCACTAAGC 
CACGATTTGG ATCACCGTGG TGTGAATAAC TCTTACATAC AGCGAAGTGA ACATCCACTT 
GCCCAGCTTT ACTGCCATTC AATCATGGAA CACCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
TTGAAAATAA TCAAGCAAGC TATTTTAGCT ACAGACCTAG CACTGTACAT TAAGAGGCGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAATT TGGAAGATCC TCATCAAAAG 
GAGTTGTTTT TGGCAATGCT GATGACAGCT TGTGATCTTT CTGCAATTAC AAAACCCTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGATCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAACCCACT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTGTA TGAGGCCCTG 
ACCCACGTGT CAGAGGACTG TTTCCCTTTG CTAGATGGCT GCAGAAAGAA CAGGCAGAAA 
TGGCAGGCCC TTGCAGAACA GCAGGAGAAG ATGCTGATTA ATGGGGAAAG CGGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_246273.2
CDS95..2566
Translation

Target ORF information:

RefSeq Version NM_033437.3
Organism Homo sapiens(human)
Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_033437.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGTCAATG CATGGTTTGC TGAGAGAGTT CACACCATCC CTGTGTGCAA GGAAGGTATC 
AGAGGCCACA CCGAATCTTG CTCTTGTCCC TTGCAGCAGA GTCCTCGTGC AGATAACAGT 
GCCCCTGGAA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTTGTCA AGGATTCTGA GGGAACTGTG AGCTTCCTCT CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCTCC AAGGTTTGAT CATGATGAAG GGGACCAGTG CTCAAGACTC 
TTGGAATTAG TGAAGGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCACAAAATT 
TTCTTGCATA TCCATGGACT GATATCTGCT GACCGCTATT CCCTGTTCCT TGTCTGTGAA 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTCTTTG ATGTTGCTGA AGGTTCAACA 
CTGGAAGAAG TTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCGC TTGGTGAGCC CTTGAACATC AAAGATGCAT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGCTACAAG ACACAAAGCA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAACGGT 
GGGACATTTA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAGCT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGTTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTTTGAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAAGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGT GTGTTTCACA TGGAGTGTGA GGAATTAGAA 
AAATCATCTG ATACATTAAC AAGGGAACAT GATGCAAACA AAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGATAAAAGA 
TTTCCCTGGA CAACTGAAAA TACAGGAAAT GTAAACCAGC AGTGCATTAG AAGTTTGCTT 
TGTACACCTA TAAAAAATGG AAAGAAGAAT AAAGTTATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGACGA ACAGTTTCTG 
GAAGCTTTTG TCATCTTTTG TGGCTTGGGG ATCCAGAACA CGCAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA AATGGTCACA TTGGAGGTTC TGTCGTATCA TGCTTCAGCA 
GCAGAGGAAG AAACAAGAGA GCTACAGTCG TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG 
ACCCTTAAAA TTACTGACTT TAGCTTCAGT GACTTTGAGC TGTCTGATCT GGAAACAGCA 
CTGTGTACAA TTCGGATGTT TACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGATGGAT TTTAAGTGTT AAGAAGAATT ATCGGAAGAA TGTTGCCTAT 
CATAATTGGA GACATGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTCT AAAAGCAGGC 
AAAATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCAT TGCTGATTGC TGCACTAAGC 
CACGATTTGG ATCACCGTGG TGTGAATAAC TCTTACATAC AGCGAAGTGA ACATCCACTT 
GCCCAGCTTT ACTGCCATTC AATCATGGAA CACCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
TTGAAAATAA TCAAGCAAGC TATTTTAGCT ACAGACCTAG CACTGTACAT TAAGAGGCGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAATT TGGAAGATCC TCATCAAAAG 
GAGTTGTTTT TGGCAATGCT GATGACAGCT TGTGATCTTT CTGCAATTAC AAAACCCTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGATCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAACCCACT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTGTA TGAGGCCCTG 
ACCCACGTGT CAGAGGACTG TTTCCCTTTG CTAGATGGCT GCAGAAAGAA CAGGCAGAAA 
TGGCAGGCCC TTGCAGAACA GCAGGAGAAG ATGCTGATTA ATGGGGAAAG CGGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02694
Clone ID Related Accession (Same CDS sequence) NM_001083.3 , NM_001083.4
Accession Version NM_001083.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-26
Organism Homo sapiens(human)
Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM920622.1, AF043731.1, AY264918.1, AC093752.2 and BM978332.1. On or before Mar 24, 2005 this sequence version replaced NM_033431.1, NM_001083.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as PDE5A1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126233.1, SRR1803611.275562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC 
CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG 
AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC 
ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT 
CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT 
TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA 
AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG 
ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA 
CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA 
CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC 
ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC 
CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG 
GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001074.2
CDS156..2783
Translation

Target ORF information:

RefSeq Version NM_001083.3
Organism Homo sapiens(human)
Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001083.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC 
CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG 
AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC 
ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT 
CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT 
TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA 
AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG 
ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA 
CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA 
CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC 
ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC 
CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG 
GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02694
Clone ID Related Accession (Same CDS sequence) NM_001083.3 , NM_001083.4
Accession Version NM_001083.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-03
Organism Homo sapiens(human)
Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF043731.1, AY264918.1, AC093752.2 and BM978332.1. On Nov 23, 2018 this sequence version replaced NM_001083.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as PDE5A1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126233.1, SRR1803611.275562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000354960.8/ ENSP00000347046.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC 
CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG 
AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC 
ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT 
CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT 
TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA 
AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG 
ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA 
CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA 
CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC 
ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC 
CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG 
GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001074.2
CDS134..2761
Translation

Target ORF information:

RefSeq Version NM_001083.4
Organism Homo sapiens(human)
Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001083.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGAACAA GCTGACTGAC 
CTGGAGATAC TTGCATTGCT GATTGCTGCA CTAAGCCACG ATTTGGATCA CCGTGGTGTG 
AATAACTCTT ACATACAGCG AAGTGAACAT CCACTTGCCC AGCTTTACTG CCATTCAATC 
ATGGAACACC ATCATTTTGA CCAGTGCCTG ATGATTCTTA ATAGTCCAGG CAATCAGATT 
CTCAGTGGCC TCTCCATTGA AGAATATAAG ACCACGTTGA AAATAATCAA GCAAGCTATT 
TTAGCTACAG ACCTAGCACT GTACATTAAG AGGCGAGGAG AATTTTTTGA ACTTATAAGA 
AAAAATCAAT TCAATTTGGA AGATCCTCAT CAAAAGGAGT TGTTTTTGGC AATGCTGATG 
ACAGCTTGTG ATCTTTCTGC AATTACAAAA CCCTGGCCTA TTCAACAACG GATAGCAGAA 
CTTGTAGCAA CTGAATTTTT TGATCAAGGA GACAGAGAGA GAAAAGAACT CAACATAGAA 
CCCACTGATC TAATGAACAG GGAGAAGAAA AACAAAATCC CAAGTATGCA AGTTGGGTTC 
ATAGATGCCA TCTGCTTGCA ACTGTATGAG GCCCTGACCC ACGTGTCAGA GGACTGTTTC 
CCTTTGCTAG ATGGCTGCAG AAAGAACAGG CAGAAATGGC AGGCCCTTGC AGAACAGCAG 
GAGAAGATGC TGATTAATGG GGAAAGCGGC CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu05453
Clone ID Related Accession (Same CDS sequence) NM_033430.3 , NM_033430.2
Accession Version NM_033430.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-03
Organism Homo sapiens(human)
Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC080089.5, AF043732.1, AY264918.1, AC093752.2 and BM978332.1. On Jun 1, 2019 this sequence version replaced NM_033430.2. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2, also known as PDE5A2) has a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.247788.1, SRR1660805.155004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC 
TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG 
AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT 
CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT 
TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT 
GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC 
TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC 
AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC 
TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT 
GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT 
GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT 
AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT 
GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT 
AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG 
CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG 
ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA 
TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG 
TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT 
AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT 
CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG 
ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC 
TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA 
CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT 
ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT 
TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA 
GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT 
GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG 
CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG 
ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_236914.2
CDS563..3064
Translation

Target ORF information:

RefSeq Version NM_033430.3
Organism Homo sapiens(human)
Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_033430.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC 
TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG 
AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT 
CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT 
TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT 
GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC 
TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC 
AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC 
TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT 
GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT 
GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT 
AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT 
GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT 
AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG 
CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG 
ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA 
TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG 
TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT 
AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT 
CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG 
ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC 
TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA 
CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT 
ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT 
TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA 
GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT 
GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG 
CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG 
ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu05453
Clone ID Related Accession (Same CDS sequence) NM_033430.3 , NM_033430.2
Accession Version NM_033430.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-22
Organism Homo sapiens(human)
Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF043732.1, AY264918.1, AC093752.2 and BM978332.1. On Mar 24, 2005 this sequence version replaced NM_033430.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2, also known as PDE5A2) has a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.247788.1, SRR1660805.155004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC 
TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG 
AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT 
CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT 
TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT 
GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC 
TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC 
AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC 
TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT 
GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT 
GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT 
AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT 
GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT 
AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG 
CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG 
ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA 
TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG 
TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT 
AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT 
CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG 
ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC 
TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA 
CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT 
ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT 
TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA 
GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT 
GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG 
CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG 
ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_236914.2
CDS113..2614
Translation

Target ORF information:

RefSeq Version NM_033430.2
Organism Homo sapiens(human)
Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_033430.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGTTGCCCT TTGGAGACAA AACAAGAGAA ATGGTCAATG CATGGTTTGC TGAGAGAGTT 
CACACCATCC CTGTGTGCAA GGAAGGTATC AGAGGCCACA CCGAATCTTG CTCTTGTCCC 
TTGCAGCAGA GTCCTCGTGC AGATAACAGT GCCCCTGGAA CACCAACCAG GAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTTGTCA AGGATTCTGA GGGAACTGTG 
AGCTTCCTCT CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCTCC AAGGTTTGAT 
CATGATGAAG GGGACCAGTG CTCAAGACTC TTGGAATTAG TGAAGGATAT TTCTAGTCAT 
TTGGATGTCA CAGCCTTATG TCACAAAATT TTCTTGCATA TCCATGGACT GATATCTGCT 
GACCGCTATT CCCTGTTCCT TGTCTGTGAA GACAGCTCCA ATGACAAGTT TCTTATCAGC 
CGCCTCTTTG ATGTTGCTGA AGGTTCAACA CTGGAAGAAG TTTCAAATAA CTGTATCCGC 
TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCGC TTGGTGAGCC CTTGAACATC 
AAAGATGCAT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGCTACAAG 
ACACAAAGCA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAACGGT GGGACATTTA CTGAAAAAGA TGAAAAGGAC 
TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAGCT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGTTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTTTGAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAAGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGT 
GTGTTTCACA TGGAGTGTGA GGAATTAGAA AAATCATCTG ATACATTAAC AAGGGAACAT 
GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT 
AATATCCCAG ATGTCAGTAA GGATAAAAGA TTTCCCTGGA CAACTGAAAA TACAGGAAAT 
GTAAACCAGC AGTGCATTAG AAGTTTGCTT TGTACACCTA TAAAAAATGG AAAGAAGAAT 
AAAGTTATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG 
CCTTTCAACC GAAATGACGA ACAGTTTCTG GAAGCTTTTG TCATCTTTTG TGGCTTGGGG 
ATCCAGAACA CGCAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA AATGGTCACA 
TTGGAGGTTC TGTCGTATCA TGCTTCAGCA GCAGAGGAAG AAACAAGAGA GCTACAGTCG 
TTAGCGGCTG CTGTGGTGCC ATCTGCCCAG ACCCTTAAAA TTACTGACTT TAGCTTCAGT 
GACTTTGAGC TGTCTGATCT GGAAACAGCA CTGTGTACAA TTCGGATGTT TACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGATGGAT TTTAAGTGTT 
AAGAAGAATT ATCGGAAGAA TGTTGCCTAT CATAATTGGA GACATGCCTT TAATACAGCT 
CAGTGCATGT TTGCTGCTCT AAAAGCAGGC AAAATTCAGA ACAAGCTGAC TGACCTGGAG 
ATACTTGCAT TGCTGATTGC TGCACTAAGC CACGATTTGG ATCACCGTGG TGTGAATAAC 
TCTTACATAC AGCGAAGTGA ACATCCACTT GCCCAGCTTT ACTGCCATTC AATCATGGAA 
CACCATCATT TTGACCAGTG CCTGATGATT CTTAATAGTC CAGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACG TTGAAAATAA TCAAGCAAGC TATTTTAGCT 
ACAGACCTAG CACTGTACAT TAAGAGGCGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAATT TGGAAGATCC TCATCAAAAG GAGTTGTTTT TGGCAATGCT GATGACAGCT 
TGTGATCTTT CTGCAATTAC AAAACCCTGG CCTATTCAAC AACGGATAGC AGAACTTGTA 
GCAACTGAAT TTTTTGATCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAACCCACT 
GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAACTGTA TGAGGCCCTG ACCCACGTGT CAGAGGACTG TTTCCCTTTG 
CTAGATGGCT GCAGAAAGAA CAGGCAGAAA TGGCAGGCCC TTGCAGAACA GCAGGAGAAG 
ATGCTGATTA ATGGGGAAAG CGGCCAGGCC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95522
Clone ID Related Accession (Same CDS sequence) XM_017008791.2 , XM_017008791.1
Accession Version XM_017008791.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG 
GGAATCGACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016864280.1
CDS37..1968
Translation

Target ORF information:

RefSeq Version XM_017008791.2
Organism Homo sapiens(human)
Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017008791.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG 
GGAATCGACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95522
Clone ID Related Accession (Same CDS sequence) XM_017008791.2 , XM_017008791.1
Accession Version XM_017008791.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG 
GGAATCGACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016864280.1
CDS410..2341
Translation

Target ORF information:

RefSeq Version XM_017008791.1
Organism Homo sapiens(human)
Definition Homo sapiens phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017008791.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGAGCGGG CCGGCCCCAG CTTCGGGCAG CAGCGACAGC AGCAGCAGCC CCAGCAGCAG 
AAGCAGCAGC AGAGGGATCA GGACTCGGTC GAAGCATGGC TGGACGATCA CTGGGACTTT 
ACCTTCTCAT ACTTTGTTAG AAAAGCCACC AGAGAAATGG TCAATGCATG GTTTGCTGAG 
AGAGTTCACA CCATCCCTGT GTGCAAGGAA GGTATCAGAG GCCACACCGA ATCTTGCTCT 
TGTCCCTTGC AGCAGAGTCC TCGTGCAGAT AACAGTGCCC CTGGAACACC AACCAGGAAA 
ATCTCTGCCT CTGAATTTGA CCGGCCTCTT AGACCCATTG TTGTCAAGGA TTCTGAGGGA 
ACTGTGAGCT TCCTCTCTGA CTCAGAAAAG AAGGAACAGA TGCCTCTAAC CCCTCCAAGG 
TTTGATCATG ATGAAGGGGA CCAGTGCTCA AGACTCTTGG AATTAGTGAA GGATATTTCT 
AGTCATTTGG ATGTCACAGC CTTATGTCAC AAAATTTTCT TGCATATCCA TGGACTGATA 
TCTGCTGACC GCTATTCCCT GTTCCTTGTC TGTGAAGACA GCTCCAATGA CAAGTTTCTT 
ATCAGCCGCC TCTTTGATGT TGCTGAAGGT TCAACACTGG AAGAAGTTTC AAATAACTGT 
ATCCGCTTAG AATGGAACAA AGGCATTGTG GGACATGTGG CAGCGCTTGG TGAGCCCTTG 
AACATCAAAG ATGCATATGA GGATCCTCGG TTCAATGCAG AAGTTGACCA AATTACAGGC 
TACAAGACAC AAAGCATTCT TTGTATGCCA ATTAAGAATC ATAGGGAAGA GGTTGTTGGT 
GTAGCCCAGG CCATCAACAA GAAATCAGGA AACGGTGGGA CATTTACTGA AAAAGATGAA 
AAGGACTTTG CTGCTTATTT GGCATTTTGT GGTATTGTTC TTCATAATGC TCAGCTCTAT 
GAGACTTCAC TGCTGGAGAA CAAGAGAAAT CAGGTGCTGC TTGACCTTGC TAGTTTAATT 
TTTGAAGAAC AACAATCATT AGAAGTAATT TTGAAGAAAA TAGCTGCCAC TATTATCTCT 
TTCATGCAAG TGCAGAAATG CACCATTTTC ATAGTGGATG AAGATTGCTC CGATTCTTTT 
TCTAGTGTGT TTCACATGGA GTGTGAGGAA TTAGAAAAAT CATCTGATAC ATTAACAAGG 
GAACATGATG CAAACAAAAT CAATTACATG TATGCTCAGT ATGTCAAAAA TACTATGGAA 
CCACTTAATA TCCCAGATGT CAGTAAGGAT AAAAGATTTC CCTGGACAAC TGAAAATACA 
GGAAATGTAA ACCAGCAGTG CATTAGAAGT TTGCTTTGTA CACCTATAAA AAATGGAAAG 
AAGAATAAAG TTATAGGGGT TTGCCAACTT GTTAATAAGA TGGAGGAGAA TACTGGCAAG 
GTTAAGCCTT TCAACCGAAA TGACGAACAG TTTCTGGAAG CTTTTGTCAT CTTTTGTGGC 
TTGGGGATCC AGAACACGCA GATGTATGAA GCAGTGGAGA GAGCCATGGC CAAGCAAATG 
GTCACATTGG AGGTTCTGTC GTATCATGCT TCAGCAGCAG AGGAAGAAAC AAGAGAGCTA 
CAGTCGTTAG CGGCTGCTGT GGTGCCATCT GCCCAGACCC TTAAAATTAC TGACTTTAGC 
TTCAGTGACT TTGAGCTGTC TGATCTGGAA ACAGCACTGT GTACAATTCG GATGTTTACT 
GACCTCAACC TTGTGCAGAA CTTCCAGATG AAACATGAGG TTCTTTGCAG ATGGATTTTA 
AGTGTTAAGA AGAATTATCG GAAGAATGTT GCCTATCATA ATTGGAGACA TGCCTTTAAT 
ACAGCTCAGT GCATGTTTGC TGCTCTAAAA GCAGGCAAAA TTCAGATTCT AACTTCAGTG 
GGAATCGACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Distinct phosphodiesterase 5A-containing compartments allow selective regulation of cGMP-dependent signalling in human arterial smooth muscle cells.
Cellular signalling36204-211(2017 Aug)
Wilson LS,Guo M,Umana MB,Maurice DH


book

Inhibition of PDE5A1 guanosine cyclic monophosphate (cGMP) hydrolysing activity by sildenafil analogues that inhibit cellular cGMP efflux.
The Journal of pharmacy and pharmacology69(6)675-683(2017 Jun)
Subbotina A,Ravna AW,Lysaa RA,Abagyan R,Bugno R,Sager G


book

Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome.
Oncotarget8(8)13223-13239(2017 Feb)
Cesarini V,Martini M,Vitiani LR,Gravina GL,Di Agostino S,Graziani G,D'Alessandris QG,Pallini R,Larocca LM,Rossi P,Jannini EA,Dolci S