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ARHGEF7 cDNA ORF clone, Homo sapiens (human)

Gene Symbol ARHGEF7
Entrez Gene ID 8874
Full Name Rho guanine nucleotide exchange factor (GEF) 7
Synonyms BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB
General protein information
Preferred Names
rho guanine nucleotide exchange factor 7
Names
rho guanine nucleotide exchange factor 7
PAK-interacting exchange factor beta
guanine nucleotide exchange factor 7
SH3 domain-containing proline-rich protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

13

13q34

Summary Rho GTPases play a fundamental role in numerous cellular processes triggered by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. This protein can induce membrane ruffling. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

605477


mRNA and Protein(s)

mRNA Protein Name
XM_005254085 XP_005254142 rho guanine nucleotide exchange factor 7 isoform X2
XM_005254086 XP_005254143 rho guanine nucleotide exchange factor 7 isoform X3
XM_011521130 XP_011519432 rho guanine nucleotide exchange factor 7 isoform X4
XM_011521131 XP_011519433 rho guanine nucleotide exchange factor 7 isoform X5
XM_006719956 XP_006720019 rho guanine nucleotide exchange factor 7 isoform X6
XM_005254087 XP_005254144 rho guanine nucleotide exchange factor 7 isoform X7
XM_005254088 XP_005254145 rho guanine nucleotide exchange factor 7 isoform X8
XM_005254089 XP_005254146 rho guanine nucleotide exchange factor 7 isoform X9
XM_005254090 XP_005254147 rho guanine nucleotide exchange factor 7 isoform X10
XM_005254091 XP_005254148 rho guanine nucleotide exchange factor 7 isoform X11
XM_005254092 XP_005254149 rho guanine nucleotide exchange factor 7 isoform X12
XM_011521132 XP_011519434 rho guanine nucleotide exchange factor 7 isoform X12
XM_006719958 XP_006720021 rho guanine nucleotide exchange factor 7 isoform X12
XM_005254093 XP_005254150 rho guanine nucleotide exchange factor 7 isoform X12
XM_005254094 XP_005254151 rho guanine nucleotide exchange factor 7 isoform X12
XM_006719959 XP_006720022 rho guanine nucleotide exchange factor 7 isoform X12
XM_006719960 XP_006720023 rho guanine nucleotide exchange factor 7 isoform X13
XM_005254095 XP_005254152 rho guanine nucleotide exchange factor 7 isoform X1
XM_011521133 XP_011519435 rho guanine nucleotide exchange factor 7 isoform X14
NM_003899 NP_003890 rho guanine nucleotide exchange factor 7 isoform a
NM_145735 NP_663788 rho guanine nucleotide exchange factor 7 isoform b
NM_001113511 NP_001106983 rho guanine nucleotide exchange factor 7 isoform c
NM_001113512 NP_001106984 rho guanine nucleotide exchange factor 7 isoform d
NM_001113513 NP_001106985 rho guanine nucleotide exchange factor 7 isoform a



Homo sapiens (human) ARHGEF7 NP_001106983.1
Pan troglodytes (chimpanzee) ARHGEF7 XP_003314260.1
Macaca mulatta (Rhesus monkey) ARHGEF7 XP_002800879.1
Canis lupus familiaris (dog) ARHGEF7 XP_005634157.1
Bos taurus (cattle) ARHGEF7 XP_005214063.1
Mus musculus (house mouse) Arhgef7 NP_001106989.1
Rattus norvegicus (Norway rat) Arhgef7 NP_001106993.1
Gallus gallus (chicken) ARHGEF7 XP_003640579.1
Danio rerio (zebrafish) arhgef7a NP_001116707.1
Caenorhabditis elegans pix-1 NP_001257227.1
Xenopus (Silurana) tropicalis (western clawed frog) arhgef7 XP_004912048.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARHGEF7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu47394 XM_005254085 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu47395 XM_005254086 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu62272 XM_011521130 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu62273 XM_011521131 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu47396 XM_006719956 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47397 XM_005254087 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47398 XM_005254088 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47400 XM_005254089 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47401 XM_005254090 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47402 XM_005254091 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47403 XM_005254092 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47403 XM_011521132 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47403 XM_006719958 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47403 XM_005254093 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47403 XM_005254094 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47403 XM_006719959 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47404 XM_006719960 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu24709 XM_005254095 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu62274 XM_011521133 PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu24709 NM_003899 Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu24860 NM_145735 Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $552.30
$789.00
OHu24807
NM_001113511 Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu24900 NM_001113512 Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu24709 NM_001113513 Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu47394
Clone ID Related Accession (Same CDS sequence) XM_005254085
Accession Version XM_005254085.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2625bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423432. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GTGAGGTCCC TTGACCACTC AAACAGCCAG
ATGACGGATC TTAGTAAGAG CCCTAAGACC ATGAAAAAGC TGCTGCCCAA GCGCAAACCT
GAACGGAAGC CTTCAGATGA GGAGTTCGCG TCCCGGAAAA GCACAGCTGC TTTGGAAGAA
GATGCTCAGA TTCTGAAAGT CATTGAAGCT TACTGCACCA GCGCCAAAAC AAGGCAAACA
CTCAATTCAA CATGGCAAGG CACTGACCTG ATGCATAATC ACGTCTTGGC TGATGATGAC
CAACCAAGCC TAGACTCCCT GGGGCGTCGC AGTAGCCTTT CTCGTTTGGA GCCTTCAGAC
CTCTCGGAAG ACTCTGACTA TGACAGTATA TGGACAGCCC ATAGTTACAG AATGGGTTCT
ACATCTCGTT CACGCAAAGA ATCTGCTCCA CAAGTTTTGC TTCCAGAAGA AGAGAAAATT
ATAGTGGAAG AAACTAAAAG TAATGGTCAG ACAGTGATAG AAGAAAAGAG TCTTGTGGAT
ACCGTATATG CATTAAAGGA TGAAGTTCAA GAATTAAGAC AGGACAACAA AAAGATGAAG
AAATCTCTAG AGGAAGAACA GAGAGCCCGC AAAGACCTGG AGAAGCTGGT GAGGAAAGTC
CTGAAGAACA TGAATGATCC TGCCTGGGAT GAGACCAATC TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254142.1
CDS37..2661
Misc Feature(1)97..357(+)
Misc Feature(2)256..354(+)
Misc Feature(3)535..696(+)
Misc Feature(4)550..684(+)
Misc Feature(5)787..1320(+)
Misc Feature(6)805..1320(+)
Misc Feature(7)1411..1707(+)
Translation

Target ORF information:

RefSeq Version XM_005254085
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254085

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GTGAGGTCCC TTGACCACTC AAACAGCCAG
ATGACGGATC TTAGTAAGAG CCCTAAGACC ATGAAAAAGC TGCTGCCCAA GCGCAAACCT
GAACGGAAGC CTTCAGATGA GGAGTTCGCG TCCCGGAAAA GCACAGCTGC TTTGGAAGAA
GATGCTCAGA TTCTGAAAGT CATTGAAGCT TACTGCACCA GCGCCAAAAC AAGGCAAACA
CTCAATTCAA CATGGCAAGG CACTGACCTG ATGCATAATC ACGTCTTGGC TGATGATGAC
CAACCAAGCC TAGACTCCCT GGGGCGTCGC AGTAGCCTTT CTCGTTTGGA GCCTTCAGAC
CTCTCGGAAG ACTCTGACTA TGACAGTATA TGGACAGCCC ATAGTTACAG AATGGGTTCT
ACATCTCGTT CACGCAAAGA ATCTGCTCCA CAAGTTTTGC TTCCAGAAGA AGAGAAAATT
ATAGTGGAAG AAACTAAAAG TAATGGTCAG ACAGTGATAG AAGAAAAGAG TCTTGTGGAT
ACCGTATATG CATTAAAGGA TGAAGTTCAA GAATTAAGAC AGGACAACAA AAAGATGAAG
AAATCTCTAG AGGAAGAACA GAGAGCCCGC AAAGACCTGG AGAAGCTGGT GAGGAAAGTC
CTGAAGAACA TGAATGATCC TGCCTGGGAT GAGACCAATC TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47395
Clone ID Related Accession (Same CDS sequence) XM_005254086
Accession Version XM_005254086.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423434. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAACATGGC AAGGCACTGA CCTGATGCAT
AATCACGTCT TGGCTGATGA TGACCAACCA AGCCTAGACT CCCTGGGGCG TCGCAGTAGC
CTTTCTCGTT TGGAGCCTTC AGACCTCTCG GAAGACTCTG ACTATGACAG TATATGGACA
GCCCATAGTT ACAGAATGGG TTCTACATCT CGTTCACGCA AAGAATCTGC TCCACAAGTT
TTGCTTCCAG AAGAAGAGAA AATTATAGTG GAAGAAACTA AAAGTAATGG TCAGACAGTG
ATAGAAGAAA AGAGTCTTGT GGATACCGTA TATGCATTAA AGGATGAAGT TCAAGAATTA
AGACAGGACA ACAAAAAGAT GAAGAAATCT CTAGAGGAAG AACAGAGAGC CCGCAAAGAC
CTGGAGAAGC TGGTGAGGAA AGTCCTGAAG AACATGAATG ATCCTGCCTG GGATGAGACC
AATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254143.1
CDS37..2625
Misc Feature(1)97..357(+)
Misc Feature(2)256..354(+)
Misc Feature(3)535..696(+)
Misc Feature(4)550..684(+)
Misc Feature(5)787..1320(+)
Misc Feature(6)805..1320(+)
Misc Feature(7)1411..1707(+)
Translation

Target ORF information:

RefSeq Version XM_005254086
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254086

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAACATGGC AAGGCACTGA CCTGATGCAT
AATCACGTCT TGGCTGATGA TGACCAACCA AGCCTAGACT CCCTGGGGCG TCGCAGTAGC
CTTTCTCGTT TGGAGCCTTC AGACCTCTCG GAAGACTCTG ACTATGACAG TATATGGACA
GCCCATAGTT ACAGAATGGG TTCTACATCT CGTTCACGCA AAGAATCTGC TCCACAAGTT
TTGCTTCCAG AAGAAGAGAA AATTATAGTG GAAGAAACTA AAAGTAATGG TCAGACAGTG
ATAGAAGAAA AGAGTCTTGT GGATACCGTA TATGCATTAA AGGATGAAGT TCAAGAATTA
AGACAGGACA ACAAAAAGAT GAAGAAATCT CTAGAGGAAG AACAGAGAGC CCGCAAAGAC
CTGGAGAAGC TGGTGAGGAA AGTCCTGAAG AACATGAATG ATCCTGCCTG GGATGAGACC
AATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62272
Clone ID Related Accession (Same CDS sequence) XM_011521130
Accession Version XM_011521130.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAACGTT TGATGCAAAT
GATTTGTATC AGGGGCAGAA TTTTAACAAG GTCCTCAGTT CCTTAGTGAC TCTAAATAAA
GTAACAGCAG ACATCGGGCT GGGGAGTGAC TCCGTGTGTG CCCGGCCCTC GTCTCACCGC
ATAAAGTCTT TTGACTCCCT TGGATCACAG TCTTTGCACA CTCGGACTTC AAAACTGTTC
CAGGGCCAGT ATCGGAGTTT GGACATGACC GATAATAGCA ACAATCAACT GGTAGTAAGA
GCAAAGTTTA ACTTCCAGCA GACCAATGAG GACGAGCTTT CCTTCTCAAA AGGAGACGTC
ATCCATGTCA CCCGTGTGGA AGAGGGAGGC TGGTGGGAGG GCACACTCAA CGGCCGGACC
GGCTGGTTCC CCAGCAACTA CGTGCGCGAG GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC
AAATCAGGAA CACTGAAGAG CCCTCCCAAA GGATTTGATA CGACTGCCAT AAACAAAAGC
TATTACAATG TGGTGCTACA GAATATTTTA GAAACAGAAA ATGAATATTC TAAAGAACTT
CAGACTGTGC TTTCAACGTA CCTACGGCCA TTGCAGACCA GTGAGAAGTT AAGTTCAGCA
AACATTTCAT ATTTAATGGG AAATCTAGAA GAAATATGTT CTTTCCAGCA AATGCTCGTA
CAGTCTTTAG AAGAATGCAC CAAGTTGCCC GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT
TTAAACCTGA TGCCACAGAT GAAAACCCTG TACCTCACGT ATTGTGCCAA TCACCCTTCT
GCAGTGAATG TCCTCACGGA ACACAGTGAG GAGTTGGGGG AGTTCATGGA GACCAAAGGT
GCCAGCAGCC CTGGGATTCT CGTGCTGACC ACGGGCCTGA GCAAACCCTT CATGCGCCTG
GATAAATACC CTACGCTGCT CAAAGAGCTC GAGAGACACA TGGAGGATTA TCATACAGAT
AGACAAGATA TTCAAAAATC CATGGCTGCC TTCAAAAACC TTTCAGCCCA ATGTCAAGAA
GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG ATCCTGACGG AAGCCATCCG GAACTGGGAG
GGCGATGACA TTAAAACTCT GGGCAACGTC ACTTACATGT CCCAGGTCCT GATTCAGTGT
GCCGGAAGTG AGGAAAAGAA TGAAAGATAT CTTCTACTCT TCCCAAATGT TTTGCTAATG
TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT ATCTATCAGG GAAAGCTTCC AACGACAGGA
ATGACAATCA CAAAGCTTGA GGACAGTGAA AATCATAGAA ATGCATTTGA AATATCAGGG
AGCATGATTG AGCGGATATT AGTGTCGTGC AACAACCAGC AGGATCTGCA GGAATGGGTG
GAGCACCTAC AGAAGCAAAC GAAGGTCACG TCTGTGGGAA ACCCCACCAT AAAGCCTCAT
TCAGTGCCAT CTCATACCCT CCCCTCCCAC CCGGTCACTC CGTCCAGCAA GCACGCAGAC
AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC CACACGCTGC CCCACCCCTC CCACCACGGC
ACCCCGCACA CCACCATCAA CTGGGGACCC CTGGAGCCTC CGAAAACACC CAAGCCCTGG
AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC CTCCGGCCCT CAGCTGCTCT CTGCTACAAG
GAGGTGAGGT CCCTTGACCA CTCAAACAGC CAGATGACGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAACATGGCA AGGCACTGAC
CTGATGCATA ATCACGTCTT GGCTGATGAT GACCAACCAA GCCTAGACTC CCTGGGGCGT
CGCAGTAGCC TTTCTCGTTT GGAGCCTTCA GACCTCTCGG AAGACTCTGA CTATGACAGT
ATATGGACAG CCCATAGTTA CAGAATGGGT TCTACATCTC GTTCACGCAA AGAATCTGCT
CCACAAGTTT TGCTTCCAGA AGAAGAGAAA ATTATAGTGG AAGAAACTAA AAGTAATGGT
CAGACAGTGA TAGAAGAAAA GAGTCTTGTG GATACCGTAT ATGCATTAAA GGATGAAGTT
CAAGAATTAA GACAGGACAA CAAAAAGATG AAGAAATCTC TAGAGGAAGA ACAGAGAGCC
CGCAAAGACC TGGAGAAGCT GGTGAGGAAA GTCCTGAAGA ACATGAATGA TCCTGCCTGG
GATGAGACCA ATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519432.1
CDS94..2631
Misc Feature(1)154..>318(+)
Misc Feature(2)505..666(+)
Misc Feature(3)520..654(+)
Misc Feature(4)757..1290(+)
Misc Feature(5)775..1290(+)
Misc Feature(6)1381..1677(+)
Translation

Target ORF information:

RefSeq Version XM_011521130
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521130

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAACGTT TGATGCAAAT
GATTTGTATC AGGGGCAGAA TTTTAACAAG GTCCTCAGTT CCTTAGTGAC TCTAAATAAA
GTAACAGCAG ACATCGGGCT GGGGAGTGAC TCCGTGTGTG CCCGGCCCTC GTCTCACCGC
ATAAAGTCTT TTGACTCCCT TGGATCACAG TCTTTGCACA CTCGGACTTC AAAACTGTTC
CAGGGCCAGT ATCGGAGTTT GGACATGACC GATAATAGCA ACAATCAACT GGTAGTAAGA
GCAAAGTTTA ACTTCCAGCA GACCAATGAG GACGAGCTTT CCTTCTCAAA AGGAGACGTC
ATCCATGTCA CCCGTGTGGA AGAGGGAGGC TGGTGGGAGG GCACACTCAA CGGCCGGACC
GGCTGGTTCC CCAGCAACTA CGTGCGCGAG GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC
AAATCAGGAA CACTGAAGAG CCCTCCCAAA GGATTTGATA CGACTGCCAT AAACAAAAGC
TATTACAATG TGGTGCTACA GAATATTTTA GAAACAGAAA ATGAATATTC TAAAGAACTT
CAGACTGTGC TTTCAACGTA CCTACGGCCA TTGCAGACCA GTGAGAAGTT AAGTTCAGCA
AACATTTCAT ATTTAATGGG AAATCTAGAA GAAATATGTT CTTTCCAGCA AATGCTCGTA
CAGTCTTTAG AAGAATGCAC CAAGTTGCCC GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT
TTAAACCTGA TGCCACAGAT GAAAACCCTG TACCTCACGT ATTGTGCCAA TCACCCTTCT
GCAGTGAATG TCCTCACGGA ACACAGTGAG GAGTTGGGGG AGTTCATGGA GACCAAAGGT
GCCAGCAGCC CTGGGATTCT CGTGCTGACC ACGGGCCTGA GCAAACCCTT CATGCGCCTG
GATAAATACC CTACGCTGCT CAAAGAGCTC GAGAGACACA TGGAGGATTA TCATACAGAT
AGACAAGATA TTCAAAAATC CATGGCTGCC TTCAAAAACC TTTCAGCCCA ATGTCAAGAA
GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG ATCCTGACGG AAGCCATCCG GAACTGGGAG
GGCGATGACA TTAAAACTCT GGGCAACGTC ACTTACATGT CCCAGGTCCT GATTCAGTGT
GCCGGAAGTG AGGAAAAGAA TGAAAGATAT CTTCTACTCT TCCCAAATGT TTTGCTAATG
TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT ATCTATCAGG GAAAGCTTCC AACGACAGGA
ATGACAATCA CAAAGCTTGA GGACAGTGAA AATCATAGAA ATGCATTTGA AATATCAGGG
AGCATGATTG AGCGGATATT AGTGTCGTGC AACAACCAGC AGGATCTGCA GGAATGGGTG
GAGCACCTAC AGAAGCAAAC GAAGGTCACG TCTGTGGGAA ACCCCACCAT AAAGCCTCAT
TCAGTGCCAT CTCATACCCT CCCCTCCCAC CCGGTCACTC CGTCCAGCAA GCACGCAGAC
AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC CACACGCTGC CCCACCCCTC CCACCACGGC
ACCCCGCACA CCACCATCAA CTGGGGACCC CTGGAGCCTC CGAAAACACC CAAGCCCTGG
AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC CTCCGGCCCT CAGCTGCTCT CTGCTACAAG
GAGGTGAGGT CCCTTGACCA CTCAAACAGC CAGATGACGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAACATGGCA AGGCACTGAC
CTGATGCATA ATCACGTCTT GGCTGATGAT GACCAACCAA GCCTAGACTC CCTGGGGCGT
CGCAGTAGCC TTTCTCGTTT GGAGCCTTCA GACCTCTCGG AAGACTCTGA CTATGACAGT
ATATGGACAG CCCATAGTTA CAGAATGGGT TCTACATCTC GTTCACGCAA AGAATCTGCT
CCACAAGTTT TGCTTCCAGA AGAAGAGAAA ATTATAGTGG AAGAAACTAA AAGTAATGGT
CAGACAGTGA TAGAAGAAAA GAGTCTTGTG GATACCGTAT ATGCATTAAA GGATGAAGTT
CAAGAATTAA GACAGGACAA CAAAAAGATG AAGAAATCTC TAGAGGAAGA ACAGAGAGCC
CGCAAAGACC TGGAGAAGCT GGTGAGGAAA GTCCTGAAGA ACATGAATGA TCCTGCCTGG
GATGAGACCA ATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62273
Clone ID Related Accession (Same CDS sequence) XM_011521131
Accession Version XM_011521131.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAACGTT TGATGCAAAT
GATTTGTATC AGGGGCAGAA TTTTAACAAG GTCCTCAGTT CCTTAGTGAC TCTAAATAAA
GTAACAGCAG ACATCGGGCT GGGGAGTGAC TCCGTGTGTG CCCGGCCCTC GTCTCACCGC
ATAAAGTCTT TTGACTCCCT TGGATCACAG TCTTTGCACA CTCGGACTTC AAAACTGTTC
CAGGGCCAGT ATCGGAGTTT GGACATGACC GATAATAGCA ACAATCAACT GGTAGTAAGA
GCAAAGTTTA ACTTCCAGCA GACCAATGAG GACGAGCTTT CCTTCTCAAA AGGAGACGTC
ATCCATGTCA CCCGTGTGGA AGAGGGAGGC TGGTGGGAGG GCACACTCAA CGGCCGGACC
GGCTGGTTCC CCAGCAACTA CGTGCGCGAG GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC
AAATCAGGAA CACTGAAGAG CCCTCCCAAA GGATTTGATA CGACTGCCAT AAACAAAAGC
TATTACAATG TGGTGCTACA GAATATTTTA GAAACAGAAA ATGAATATTC TAAAGAACTT
CAGACTGTGC TTTCAACGTA CCTACGGCCA TTGCAGACCA GTGAGAAGTT AAGTTCAGCA
AACATTTCAT ATTTAATGGG AAATCTAGAA GAAATATGTT CTTTCCAGCA AATGCTCGTA
CAGTCTTTAG AAGAATGCAC CAAGTTGCCC GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT
TTAAACCTGA TGCCACAGAT GAAAACCCTG TACCTCACGT ATTGTGCCAA TCACCCTTCT
GCAGTGAATG TCCTCACGGA ACACAGTGAG GAGTTGGGGG AGTTCATGGA GACCAAAGGT
GCCAGCAGCC CTGGGATTCT CGTGCTGACC ACGGGCCTGA GCAAACCCTT CATGCGCCTG
GATAAATACC CTACGCTGCT CAAAGAGCTC GAGAGACACA TGGAGGATTA TCATACAGAT
AGACAAGATA TTCAAAAATC CATGGCTGCC TTCAAAAACC TTTCAGCCCA ATGTCAAGAA
GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG ATCCTGACGG AAGCCATCCG GAACTGGGAG
GGCGATGACA TTAAAACTCT GGGCAACGTC ACTTACATGT CCCAGGTCCT GATTCAGTGT
GCCGGAAGTG AGGAAAAGAA TGAAAGATAT CTTCTACTCT TCCCAAATGT TTTGCTAATG
TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT ATCTATCAGG GAAAGCTTCC AACGACAGGA
ATGACAATCA CAAAGCTTGA GGACAGTGAA AATCATAGAA ATGCATTTGA AATATCAGGG
AGCATGATTG AGCGGATATT AGTGTCGTGC AACAACCAGC AGGATCTGCA GGAATGGGTG
GAGCACCTAC AGAAGCAAAC GAAGGTCACG TCTGTGGGAA ACCCCACCAT AAAGCCTCAT
TCAGTGCCAT CTCATACCCT CCCCTCCCAC CCGGTCACTC CGTCCAGCAA GCACGCAGAC
AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC CACACGCTGC CCCACCCCTC CCACCACGGC
ACCCCGCACA CCACCATCAA CTGGGGACCC CTGGAGCCTC CGAAAACACC CAAGCCCTGG
AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC CTCCGGCCCT CAGCTGCTCT CTGCTACAAG
GAGGATCTTA GTAAGAGCCC TAAGACCATG AAAAAGCTGC TGCCCAAGCG CAAACCTGAA
CGGAAGCCTT CAGATGAGGA GTTCGCGTCC CGGAAAAGCA CAGCTGCTTT GGAAGAAGAT
GCTCAGATTC TGAAAGTCAT TGAAGCTTAC TGCACCAGCG CCAAAACAAG GCAAACACTC
AATTCAACAT GGCAAGGCAC TGACCTGATG CATAATCACG TCTTGGCTGA TGATGACCAA
CCAAGCCTAG ACTCCCTGGG GCGTCGCAGT AGCCTTTCTC GTTTGGAGCC TTCAGACCTC
TCGGAAGACT CTGACTATGA CAGTATATGG ACAGCCCATA GTTACAGAAT GGGTTCTACA
TCTCGTTCAC GCAAAGAATC TGCTCCACAA GTTTTGCTTC CAGAAGAAGA GAAAATTATA
GTGGAAGAAA CTAAAAGTAA TGGTCAGACA GTGATAGAAG AAAAGAGTCT TGTGGATACC
GTATATGCAT TAAAGGATGA AGTTCAAGAA TTAAGACAGG ACAACAAAAA GATGAAGAAA
TCTCTAGAGG AAGAACAGAG AGCCCGCAAA GACCTGGAGA AGCTGGTGAG GAAAGTCCTG
AAGAACATGA ATGATCCTGC CTGGGATGAG ACCAATCTAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519433.1
CDS245..2746
Misc Feature(1)305..>469(+)
Misc Feature(2)656..817(+)
Misc Feature(3)671..805(+)
Misc Feature(4)908..1441(+)
Misc Feature(5)926..1441(+)
Misc Feature(6)1532..1828(+)
Translation

Target ORF information:

RefSeq Version XM_011521131
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521131

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAACGTT TGATGCAAAT
GATTTGTATC AGGGGCAGAA TTTTAACAAG GTCCTCAGTT CCTTAGTGAC TCTAAATAAA
GTAACAGCAG ACATCGGGCT GGGGAGTGAC TCCGTGTGTG CCCGGCCCTC GTCTCACCGC
ATAAAGTCTT TTGACTCCCT TGGATCACAG TCTTTGCACA CTCGGACTTC AAAACTGTTC
CAGGGCCAGT ATCGGAGTTT GGACATGACC GATAATAGCA ACAATCAACT GGTAGTAAGA
GCAAAGTTTA ACTTCCAGCA GACCAATGAG GACGAGCTTT CCTTCTCAAA AGGAGACGTC
ATCCATGTCA CCCGTGTGGA AGAGGGAGGC TGGTGGGAGG GCACACTCAA CGGCCGGACC
GGCTGGTTCC CCAGCAACTA CGTGCGCGAG GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC
AAATCAGGAA CACTGAAGAG CCCTCCCAAA GGATTTGATA CGACTGCCAT AAACAAAAGC
TATTACAATG TGGTGCTACA GAATATTTTA GAAACAGAAA ATGAATATTC TAAAGAACTT
CAGACTGTGC TTTCAACGTA CCTACGGCCA TTGCAGACCA GTGAGAAGTT AAGTTCAGCA
AACATTTCAT ATTTAATGGG AAATCTAGAA GAAATATGTT CTTTCCAGCA AATGCTCGTA
CAGTCTTTAG AAGAATGCAC CAAGTTGCCC GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT
TTAAACCTGA TGCCACAGAT GAAAACCCTG TACCTCACGT ATTGTGCCAA TCACCCTTCT
GCAGTGAATG TCCTCACGGA ACACAGTGAG GAGTTGGGGG AGTTCATGGA GACCAAAGGT
GCCAGCAGCC CTGGGATTCT CGTGCTGACC ACGGGCCTGA GCAAACCCTT CATGCGCCTG
GATAAATACC CTACGCTGCT CAAAGAGCTC GAGAGACACA TGGAGGATTA TCATACAGAT
AGACAAGATA TTCAAAAATC CATGGCTGCC TTCAAAAACC TTTCAGCCCA ATGTCAAGAA
GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG ATCCTGACGG AAGCCATCCG GAACTGGGAG
GGCGATGACA TTAAAACTCT GGGCAACGTC ACTTACATGT CCCAGGTCCT GATTCAGTGT
GCCGGAAGTG AGGAAAAGAA TGAAAGATAT CTTCTACTCT TCCCAAATGT TTTGCTAATG
TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT ATCTATCAGG GAAAGCTTCC AACGACAGGA
ATGACAATCA CAAAGCTTGA GGACAGTGAA AATCATAGAA ATGCATTTGA AATATCAGGG
AGCATGATTG AGCGGATATT AGTGTCGTGC AACAACCAGC AGGATCTGCA GGAATGGGTG
GAGCACCTAC AGAAGCAAAC GAAGGTCACG TCTGTGGGAA ACCCCACCAT AAAGCCTCAT
TCAGTGCCAT CTCATACCCT CCCCTCCCAC CCGGTCACTC CGTCCAGCAA GCACGCAGAC
AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC CACACGCTGC CCCACCCCTC CCACCACGGC
ACCCCGCACA CCACCATCAA CTGGGGACCC CTGGAGCCTC CGAAAACACC CAAGCCCTGG
AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC CTCCGGCCCT CAGCTGCTCT CTGCTACAAG
GAGGATCTTA GTAAGAGCCC TAAGACCATG AAAAAGCTGC TGCCCAAGCG CAAACCTGAA
CGGAAGCCTT CAGATGAGGA GTTCGCGTCC CGGAAAAGCA CAGCTGCTTT GGAAGAAGAT
GCTCAGATTC TGAAAGTCAT TGAAGCTTAC TGCACCAGCG CCAAAACAAG GCAAACACTC
AATTCAACAT GGCAAGGCAC TGACCTGATG CATAATCACG TCTTGGCTGA TGATGACCAA
CCAAGCCTAG ACTCCCTGGG GCGTCGCAGT AGCCTTTCTC GTTTGGAGCC TTCAGACCTC
TCGGAAGACT CTGACTATGA CAGTATATGG ACAGCCCATA GTTACAGAAT GGGTTCTACA
TCTCGTTCAC GCAAAGAATC TGCTCCACAA GTTTTGCTTC CAGAAGAAGA GAAAATTATA
GTGGAAGAAA CTAAAAGTAA TGGTCAGACA GTGATAGAAG AAAAGAGTCT TGTGGATACC
GTATATGCAT TAAAGGATGA AGTTCAAGAA TTAAGACAGG ACAACAAAAA GATGAAGAAA
TCTCTAGAGG AAGAACAGAG AGCCCGCAAA GACCTGGAGA AGCTGGTGAG GAAAGTCCTG
AAGAACATGA ATGATCCTGC CTGGGATGAG ACCAATCTAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47396
Clone ID Related Accession (Same CDS sequence) XM_006719956
Accession Version XM_006719956.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825276. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GTGAGGTCCC TTGACCACTC AAACAGCCAG
ATGACGGATC TTAGTAAGAG CCCTAAGACC ATGAAAAAGC TGCTGCCCAA GCGCAAACCT
GAACGGAAGC CTTCAGATGA GGAGTTCGCG TCCCGGAAAA GCACAGCTGC TTTGGAAGAA
GATGCTCAGA TTCTGAAAGT CATTGAAGCT TACTGCACCA GCGCCAAAAC AAGGCAAACA
CTCAATTCAA GTTCACGCAA AGAATCTGCT CCACAAGTTT TGCTTCCAGA AGAAGAGAAA
ATTATAGTGG AAGAAACTAA AAGTAATGGT CAGACAGTGA TAGAAGAAAA GAGTCTTGTG
GATACCGTAT ATGCATTAAA GGATGAAGTT CAAGAATTAA GACAGGACAA CAAAAAGATG
AAGAAATCTC TAGAGGAAGA ACAGAGAGCC CGCAAAGACC TGGAGAAGCT GGTGAGGAAA
GTCCTGAAGA ACATGAATGA TCCTGCCTGG GATGAGACCA ATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720019.1
CDS37..2484
Misc Feature(1)97..357(+)
Misc Feature(2)256..354(+)
Misc Feature(3)535..696(+)
Misc Feature(4)550..684(+)
Misc Feature(5)787..1320(+)
Misc Feature(6)805..1320(+)
Misc Feature(7)1411..1707(+)
Translation

Target ORF information:

RefSeq Version XM_006719956
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719956

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GTGAGGTCCC TTGACCACTC AAACAGCCAG
ATGACGGATC TTAGTAAGAG CCCTAAGACC ATGAAAAAGC TGCTGCCCAA GCGCAAACCT
GAACGGAAGC CTTCAGATGA GGAGTTCGCG TCCCGGAAAA GCACAGCTGC TTTGGAAGAA
GATGCTCAGA TTCTGAAAGT CATTGAAGCT TACTGCACCA GCGCCAAAAC AAGGCAAACA
CTCAATTCAA GTTCACGCAA AGAATCTGCT CCACAAGTTT TGCTTCCAGA AGAAGAGAAA
ATTATAGTGG AAGAAACTAA AAGTAATGGT CAGACAGTGA TAGAAGAAAA GAGTCTTGTG
GATACCGTAT ATGCATTAAA GGATGAAGTT CAAGAATTAA GACAGGACAA CAAAAAGATG
AAGAAATCTC TAGAGGAAGA ACAGAGAGCC CGCAAAGACC TGGAGAAGCT GGTGAGGAAA
GTCCTGAAGA ACATGAATGA TCCTGCCTGG GATGAGACCA ATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47397
Clone ID Related Accession (Same CDS sequence) XM_005254087
Accession Version XM_005254087.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423436. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAAGTTCAC GCAAAGAATC TGCTCCACAA
GTTTTGCTTC CAGAAGAAGA GAAAATTATA GTGGAAGAAA CTAAAAGTAA TGGTCAGACA
GTGATAGAAG AAAAGAGTCT TGTGGATACC GTATATGCAT TAAAGGATGA AGTTCAAGAA
TTAAGACAGG ACAACAAAAA GATGAAGAAA TCTCTAGAGG AAGAACAGAG AGCCCGCAAA
GACCTGGAGA AGCTGGTGAG GAAAGTCCTG AAGAACATGA ATGATCCTGC CTGGGATGAG
ACCAATCTAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254144.1
CDS37..2448
Misc Feature(1)97..357(+)
Misc Feature(2)256..354(+)
Misc Feature(3)535..696(+)
Misc Feature(4)550..684(+)
Misc Feature(5)787..1320(+)
Misc Feature(6)805..1320(+)
Misc Feature(7)1411..1707(+)
Translation

Target ORF information:

RefSeq Version XM_005254087
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254087

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAAGTTCAC GCAAAGAATC TGCTCCACAA
GTTTTGCTTC CAGAAGAAGA GAAAATTATA GTGGAAGAAA CTAAAAGTAA TGGTCAGACA
GTGATAGAAG AAAAGAGTCT TGTGGATACC GTATATGCAT TAAAGGATGA AGTTCAAGAA
TTAAGACAGG ACAACAAAAA GATGAAGAAA TCTCTAGAGG AAGAACAGAG AGCCCGCAAA
GACCTGGAGA AGCTGGTGAG GAAAGTCCTG AAGAACATGA ATGATCCTGC CTGGGATGAG
ACCAATCTAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47398
Clone ID Related Accession (Same CDS sequence) XM_005254088
Accession Version XM_005254088.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2409bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423438. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGGACATGAC CGATAATAGC AACAATCAAC TGGTAGTAAG AGCAAAGTTT
AACTTCCAGC AGACCAATGA GGACGAGCTT TCCTTCTCAA AAGGAGACGT CATCCATGTC
ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG GGCACACTCA ACGGCCGGAC CGGCTGGTTC
CCCAGCAACT ACGTGCGCGA GGTCAAGGCC AGCGAGAAGC CTGTGTCTCC CAAATCAGGA
ACACTGAAGA GCCCTCCCAA AGGATTTGAT ACGACTGCCA TAAACAAAAG CTATTACAAT
GTGGTGCTAC AGAATATTTT AGAAACAGAA AATGAATATT CTAAAGAACT TCAGACTGTG
CTTTCAACGT ACCTACGGCC ATTGCAGACC AGTGAGAAGT TAAGTTCAGC AAACATTTCA
TATTTAATGG GAAATCTAGA AGAAATATGT TCTTTCCAGC AAATGCTCGT ACAGTCTTTA
GAAGAATGCA CCAAGTTGCC CGAAGCTCAG CAGAGAGTCG GAGGCTGCTT TTTAAACCTG
ATGCCACAGA TGAAAACCCT GTACCTCACG TATTGTGCCA ATCACCCTTC TGCAGTGAAT
GTCCTCACGG AACACAGTGA GGAGTTGGGG GAGTTCATGG AGACCAAAGG TGCCAGCAGC
CCTGGGATTC TCGTGCTGAC CACGGGCCTG AGCAAACCCT TCATGCGCCT GGATAAATAC
CCTACGCTGC TCAAAGAGCT CGAGAGACAC ATGGAGGATT ATCATACAGA TAGACAAGAT
ATTCAAAAAT CCATGGCTGC CTTCAAAAAC CTTTCAGCCC AATGTCAAGA AGTCCGGAAG
AGGAAAGAGC TTGAGCTGCA GATCCTGACG GAAGCCATCC GGAACTGGGA GGGCGATGAC
ATTAAAACTC TGGGCAACGT CACTTACATG TCCCAGGTCC TGATTCAGTG TGCCGGAAGT
GAGGAAAAGA ATGAAAGATA TCTTCTACTC TTCCCAAATG TTTTGCTAAT GTTGTCTGCC
AGTCCTAGGA TGAGTGGCTT TATCTATCAG GGAAAGCTTC CAACGACAGG AATGACAATC
ACAAAGCTTG AGGACAGTGA AAATCATAGA AATGCATTTG AAATATCAGG GAGCATGATT
GAGCGGATAT TAGTGTCGTG CAACAACCAG CAGGATCTGC AGGAATGGGT GGAGCACCTA
CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA AACCCCACCA TAAAGCCTCA TTCAGTGCCA
TCTCATACCC TCCCCTCCCA CCCGGTCACT CCGTCCAGCA AGCACGCAGA CAGCAAGCCC
GCGCCGCTGA CGCCCGCCTA CCACACGCTG CCCCACCCCT CCCACCACGG CACCCCGCAC
ACCACCATCA ACTGGGGACC CCTGGAGCCT CCGAAAACAC CCAAGCCCTG GAGCCTGAGC
TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC TCAGCTGCTC TCTGCTACAA GGAGGTGAGG
TCCCTTGACC ACTCAAACAG CCAGATGACG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAACATGGC AAGGCACTGA CCTGATGCAT
AATCACGTCT TGGCTGATGA TGACCAACCA AGCCTAGACT CCCTGGGGCG TCGCAGTAGC
CTTTCTCGTT TGGAGCCTTC AGACCTCTCG GAAGACTCTG ACTATGACAG TATATGGACA
GCCCATAGTT ACAGAATGGG TTCTACATCT CGTTCACGCA AAGAATCTGC TCCACAAGTT
TTGCTTCCAG AAGAAGAGAA AATTATAGTG GAAGAAACTA AAAGTAATGG TCAGACAGTG
ATAGAAGAAA AGAGTCTTGT GGATACCGTA TATGCATTAA AGGATGAAGT TCAAGAATTA
AGACAGGACA ACAAAAAGAT GAAGAAATCT CTAGAGGAAG AACAGAGAGC CCGCAAAGAC
CTGGAGAAGC TGGTGAGGAA AGTCCTGAAG AACATGAATG ATCCTGCCTG GGATGAGACC
AATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254145.1
CDS246..2654
Misc Feature(1)306..533(+)
Misc Feature(2)528..689(+)
Misc Feature(3)543..677(+)
Misc Feature(4)780..1313(+)
Misc Feature(5)798..1313(+)
Misc Feature(6)1404..1700(+)
Translation

Target ORF information:

RefSeq Version XM_005254088
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254088

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGGACATGAC CGATAATAGC AACAATCAAC TGGTAGTAAG AGCAAAGTTT
AACTTCCAGC AGACCAATGA GGACGAGCTT TCCTTCTCAA AAGGAGACGT CATCCATGTC
ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG GGCACACTCA ACGGCCGGAC CGGCTGGTTC
CCCAGCAACT ACGTGCGCGA GGTCAAGGCC AGCGAGAAGC CTGTGTCTCC CAAATCAGGA
ACACTGAAGA GCCCTCCCAA AGGATTTGAT ACGACTGCCA TAAACAAAAG CTATTACAAT
GTGGTGCTAC AGAATATTTT AGAAACAGAA AATGAATATT CTAAAGAACT TCAGACTGTG
CTTTCAACGT ACCTACGGCC ATTGCAGACC AGTGAGAAGT TAAGTTCAGC AAACATTTCA
TATTTAATGG GAAATCTAGA AGAAATATGT TCTTTCCAGC AAATGCTCGT ACAGTCTTTA
GAAGAATGCA CCAAGTTGCC CGAAGCTCAG CAGAGAGTCG GAGGCTGCTT TTTAAACCTG
ATGCCACAGA TGAAAACCCT GTACCTCACG TATTGTGCCA ATCACCCTTC TGCAGTGAAT
GTCCTCACGG AACACAGTGA GGAGTTGGGG GAGTTCATGG AGACCAAAGG TGCCAGCAGC
CCTGGGATTC TCGTGCTGAC CACGGGCCTG AGCAAACCCT TCATGCGCCT GGATAAATAC
CCTACGCTGC TCAAAGAGCT CGAGAGACAC ATGGAGGATT ATCATACAGA TAGACAAGAT
ATTCAAAAAT CCATGGCTGC CTTCAAAAAC CTTTCAGCCC AATGTCAAGA AGTCCGGAAG
AGGAAAGAGC TTGAGCTGCA GATCCTGACG GAAGCCATCC GGAACTGGGA GGGCGATGAC
ATTAAAACTC TGGGCAACGT CACTTACATG TCCCAGGTCC TGATTCAGTG TGCCGGAAGT
GAGGAAAAGA ATGAAAGATA TCTTCTACTC TTCCCAAATG TTTTGCTAAT GTTGTCTGCC
AGTCCTAGGA TGAGTGGCTT TATCTATCAG GGAAAGCTTC CAACGACAGG AATGACAATC
ACAAAGCTTG AGGACAGTGA AAATCATAGA AATGCATTTG AAATATCAGG GAGCATGATT
GAGCGGATAT TAGTGTCGTG CAACAACCAG CAGGATCTGC AGGAATGGGT GGAGCACCTA
CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA AACCCCACCA TAAAGCCTCA TTCAGTGCCA
TCTCATACCC TCCCCTCCCA CCCGGTCACT CCGTCCAGCA AGCACGCAGA CAGCAAGCCC
GCGCCGCTGA CGCCCGCCTA CCACACGCTG CCCCACCCCT CCCACCACGG CACCCCGCAC
ACCACCATCA ACTGGGGACC CCTGGAGCCT CCGAAAACAC CCAAGCCCTG GAGCCTGAGC
TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC TCAGCTGCTC TCTGCTACAA GGAGGTGAGG
TCCCTTGACC ACTCAAACAG CCAGATGACG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAACATGGC AAGGCACTGA CCTGATGCAT
AATCACGTCT TGGCTGATGA TGACCAACCA AGCCTAGACT CCCTGGGGCG TCGCAGTAGC
CTTTCTCGTT TGGAGCCTTC AGACCTCTCG GAAGACTCTG ACTATGACAG TATATGGACA
GCCCATAGTT ACAGAATGGG TTCTACATCT CGTTCACGCA AAGAATCTGC TCCACAAGTT
TTGCTTCCAG AAGAAGAGAA AATTATAGTG GAAGAAACTA AAAGTAATGG TCAGACAGTG
ATAGAAGAAA AGAGTCTTGT GGATACCGTA TATGCATTAA AGGATGAAGT TCAAGAATTA
AGACAGGACA ACAAAAAGAT GAAGAAATCT CTAGAGGAAG AACAGAGAGC CCGCAAAGAC
CTGGAGAAGC TGGTGAGGAA AGTCCTGAAG AACATGAATG ATCCTGCCTG GGATGAGACC
AATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47400
Clone ID Related Accession (Same CDS sequence) XM_005254089
Accession Version XM_005254089.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2379bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423440. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAAGACGT TTGATGCAAA TGATTTGTAT CAGGGGCAGA ATTTTAACAA GGTCCTCAGT 
TCCTTAGTGA CTCTAAATAA AGTAACAGCA GACATCGGGC TGGGGAGTGA CTCCGTGTGT
GCCCGGCCCT CGTCTCACCG CATAAAGTCT TTTGACTCCC TTGGATCACA GTCTTTGCAC
ACTCGGACTT CAAAACTGTT CCAGGGCCAG TATCGGAGTT TGGACATGAC CGATAATAGC
AACAATCAAC TGGTAGTAAG AGCAAAGTTT AACTTCCAGC AGACCAATGA GGACGAGCTT
TCCTTCTCAA AAGGAGACGT CATCCATGTC ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG
GGCACACTCA ACGGCCGGAC CGGCTGGTTC CCCAGCAACT ACGTGCGCGA GGTCAAGGCC
AGCGAGAAGC CTGTGTCTCC CAAATCAGGA ACACTGAAGA GCCCTCCCAA AGGATTTGAT
ACGACTGCCA TAAACAAAAG CTATTACAAT GTGGTGCTAC AGAATATTTT AGAAACAGAA
AATGAATATT CTAAAGAACT TCAGACTGTG CTTTCAACGT ACCTACGGCC ATTGCAGACC
AGTGAGAAGT TAAGTTCAGC AAACATTTCA TATTTAATGG GAAATCTAGA AGAAATATGT
TCTTTCCAGC AAATGCTCGT ACAGTCTTTA GAAGAATGCA CCAAGTTGCC CGAAGCTCAG
CAGAGAGTCG GAGGCTGCTT TTTAAACCTG ATGCCACAGA TGAAAACCCT GTACCTCACG
TATTGTGCCA ATCACCCTTC TGCAGTGAAT GTCCTCACGG AACACAGTGA GGAGTTGGGG
GAGTTCATGG AGACCAAAGG TGCCAGCAGC CCTGGGATTC TCGTGCTGAC CACGGGCCTG
AGCAAACCCT TCATGCGCCT GGATAAATAC CCTACGCTGC TCAAAGAGCT CGAGAGACAC
ATGGAGGATT ATCATACAGA TAGACAAGAT ATTCAAAAAT CCATGGCTGC CTTCAAAAAC
CTTTCAGCCC AATGTCAAGA AGTCCGGAAG AGGAAAGAGC TTGAGCTGCA GATCCTGACG
GAAGCCATCC GGAACTGGGA GGGCGATGAC ATTAAAACTC TGGGCAACGT CACTTACATG
TCCCAGGTCC TGATTCAGTG TGCCGGAAGT GAGGAAAAGA ATGAAAGATA TCTTCTACTC
TTCCCAAATG TTTTGCTAAT GTTGTCTGCC AGTCCTAGGA TGAGTGGCTT TATCTATCAG
GGAAAGCTTC CAACGACAGG AATGACAATC ACAAAGCTTG AGGACAGTGA AAATCATAGA
AATGCATTTG AAATATCAGG GAGCATGATT GAGCGGATAT TAGTGTCGTG CAACAACCAG
CAGGATCTGC AGGAATGGGT GGAGCACCTA CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA
AACCCCACCA TAAAGCCTCA TTCAGTGCCA TCTCATACCC TCCCCTCCCA CCCGGTCACT
CCGTCCAGCA AGCACGCAGA CAGCAAGCCC GCGCCGCTGA CGCCCGCCTA CCACACGCTG
CCCCACCCCT CCCACCACGG CACCCCGCAC ACCACCATCA ACTGGGGACC CCTGGAGCCT
CCGAAAACAC CCAAGCCCTG GAGCCTGAGC TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC
TCAGCTGCTC TCTGCTACAA GGAGGTGAGG TCCCTTGACC ACTCAAACAG CCAGATGACG
GATCTTAGTA AGAGCCCTAA GACCATGAAA AAGCTGCTGC CCAAGCGCAA ACCTGAACGG
AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG AAAAGCACAG CTGCTTTGGA AGAAGATGCT
CAGATTCTGA AAGTCATTGA AGCTTACTGC ACCAGCGCCA AAACAAGGCA AACACTCAAT
TCAACATGGC AAGGCACTGA CCTGATGCAT AATCACGTCT TGGCTGATGA TGACCAACCA
AGCCTAGACT CCCTGGGGCG TCGCAGTAGC CTTTCTCGTT TGGAGCCTTC AGACCTCTCG
GAAGACTCTG ACTATGACAG TATATGGACA GCCCATAGTT ACAGAATGGG TTCTACATCT
CGTTCACGCA AAGAATCTGC TCCACAAGTT TTGCTTCCAG AAGAAGAGAA AATTATAGTG
GAAGAAACTA AAAGTAATGG TCAGACAGTG ATAGAAGAAA AGAGTCTTGT GGATACCGTA
TATGCATTAA AGGATGAAGT TCAAGAATTA AGACAGGACA ACAAAAAGAT GAAGAAATCT
CTAGAGGAAG AACAGAGAGC CCGCAAAGAC CTGGAGAAGC TGGTGAGGAA AGTCCTGAAG
AACATGAATG ATCCTGCCTG GGATGAGACC AATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254146.1
CDS51..2429
Misc Feature(1)303..464(+)
Misc Feature(2)318..452(+)
Misc Feature(3)555..1088(+)
Misc Feature(4)573..1088(+)
Misc Feature(5)1179..1475(+)
Translation

Target ORF information:

RefSeq Version XM_005254089
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254089

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAAGACGT TTGATGCAAA TGATTTGTAT CAGGGGCAGA ATTTTAACAA GGTCCTCAGT 
TCCTTAGTGA CTCTAAATAA AGTAACAGCA GACATCGGGC TGGGGAGTGA CTCCGTGTGT
GCCCGGCCCT CGTCTCACCG CATAAAGTCT TTTGACTCCC TTGGATCACA GTCTTTGCAC
ACTCGGACTT CAAAACTGTT CCAGGGCCAG TATCGGAGTT TGGACATGAC CGATAATAGC
AACAATCAAC TGGTAGTAAG AGCAAAGTTT AACTTCCAGC AGACCAATGA GGACGAGCTT
TCCTTCTCAA AAGGAGACGT CATCCATGTC ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG
GGCACACTCA ACGGCCGGAC CGGCTGGTTC CCCAGCAACT ACGTGCGCGA GGTCAAGGCC
AGCGAGAAGC CTGTGTCTCC CAAATCAGGA ACACTGAAGA GCCCTCCCAA AGGATTTGAT
ACGACTGCCA TAAACAAAAG CTATTACAAT GTGGTGCTAC AGAATATTTT AGAAACAGAA
AATGAATATT CTAAAGAACT TCAGACTGTG CTTTCAACGT ACCTACGGCC ATTGCAGACC
AGTGAGAAGT TAAGTTCAGC AAACATTTCA TATTTAATGG GAAATCTAGA AGAAATATGT
TCTTTCCAGC AAATGCTCGT ACAGTCTTTA GAAGAATGCA CCAAGTTGCC CGAAGCTCAG
CAGAGAGTCG GAGGCTGCTT TTTAAACCTG ATGCCACAGA TGAAAACCCT GTACCTCACG
TATTGTGCCA ATCACCCTTC TGCAGTGAAT GTCCTCACGG AACACAGTGA GGAGTTGGGG
GAGTTCATGG AGACCAAAGG TGCCAGCAGC CCTGGGATTC TCGTGCTGAC CACGGGCCTG
AGCAAACCCT TCATGCGCCT GGATAAATAC CCTACGCTGC TCAAAGAGCT CGAGAGACAC
ATGGAGGATT ATCATACAGA TAGACAAGAT ATTCAAAAAT CCATGGCTGC CTTCAAAAAC
CTTTCAGCCC AATGTCAAGA AGTCCGGAAG AGGAAAGAGC TTGAGCTGCA GATCCTGACG
GAAGCCATCC GGAACTGGGA GGGCGATGAC ATTAAAACTC TGGGCAACGT CACTTACATG
TCCCAGGTCC TGATTCAGTG TGCCGGAAGT GAGGAAAAGA ATGAAAGATA TCTTCTACTC
TTCCCAAATG TTTTGCTAAT GTTGTCTGCC AGTCCTAGGA TGAGTGGCTT TATCTATCAG
GGAAAGCTTC CAACGACAGG AATGACAATC ACAAAGCTTG AGGACAGTGA AAATCATAGA
AATGCATTTG AAATATCAGG GAGCATGATT GAGCGGATAT TAGTGTCGTG CAACAACCAG
CAGGATCTGC AGGAATGGGT GGAGCACCTA CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA
AACCCCACCA TAAAGCCTCA TTCAGTGCCA TCTCATACCC TCCCCTCCCA CCCGGTCACT
CCGTCCAGCA AGCACGCAGA CAGCAAGCCC GCGCCGCTGA CGCCCGCCTA CCACACGCTG
CCCCACCCCT CCCACCACGG CACCCCGCAC ACCACCATCA ACTGGGGACC CCTGGAGCCT
CCGAAAACAC CCAAGCCCTG GAGCCTGAGC TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC
TCAGCTGCTC TCTGCTACAA GGAGGTGAGG TCCCTTGACC ACTCAAACAG CCAGATGACG
GATCTTAGTA AGAGCCCTAA GACCATGAAA AAGCTGCTGC CCAAGCGCAA ACCTGAACGG
AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG AAAAGCACAG CTGCTTTGGA AGAAGATGCT
CAGATTCTGA AAGTCATTGA AGCTTACTGC ACCAGCGCCA AAACAAGGCA AACACTCAAT
TCAACATGGC AAGGCACTGA CCTGATGCAT AATCACGTCT TGGCTGATGA TGACCAACCA
AGCCTAGACT CCCTGGGGCG TCGCAGTAGC CTTTCTCGTT TGGAGCCTTC AGACCTCTCG
GAAGACTCTG ACTATGACAG TATATGGACA GCCCATAGTT ACAGAATGGG TTCTACATCT
CGTTCACGCA AAGAATCTGC TCCACAAGTT TTGCTTCCAG AAGAAGAGAA AATTATAGTG
GAAGAAACTA AAAGTAATGG TCAGACAGTG ATAGAAGAAA AGAGTCTTGT GGATACCGTA
TATGCATTAA AGGATGAAGT TCAAGAATTA AGACAGGACA ACAAAAAGAT GAAGAAATCT
CTAGAGGAAG AACAGAGAGC CCGCAAAGAC CTGGAGAAGC TGGTGAGGAA AGTCCTGAAG
AACATGAATG ATCCTGCCTG GGATGAGACC AATCTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47401
Clone ID Related Accession (Same CDS sequence) XM_005254090
Accession Version XM_005254090.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423442. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGGACATGAC CGATAATAGC AACAATCAAC TGGTAGTAAG AGCAAAGTTT
AACTTCCAGC AGACCAATGA GGACGAGCTT TCCTTCTCAA AAGGAGACGT CATCCATGTC
ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG GGCACACTCA ACGGCCGGAC CGGCTGGTTC
CCCAGCAACT ACGTGCGCGA GGTCAAGGCC AGCGAGAAGC CTGTGTCTCC CAAATCAGGA
ACACTGAAGA GCCCTCCCAA AGGATTTGAT ACGACTGCCA TAAACAAAAG CTATTACAAT
GTGGTGCTAC AGAATATTTT AGAAACAGAA AATGAATATT CTAAAGAACT TCAGACTGTG
CTTTCAACGT ACCTACGGCC ATTGCAGACC AGTGAGAAGT TAAGTTCAGC AAACATTTCA
TATTTAATGG GAAATCTAGA AGAAATATGT TCTTTCCAGC AAATGCTCGT ACAGTCTTTA
GAAGAATGCA CCAAGTTGCC CGAAGCTCAG CAGAGAGTCG GAGGCTGCTT TTTAAACCTG
ATGCCACAGA TGAAAACCCT GTACCTCACG TATTGTGCCA ATCACCCTTC TGCAGTGAAT
GTCCTCACGG AACACAGTGA GGAGTTGGGG GAGTTCATGG AGACCAAAGG TGCCAGCAGC
CCTGGGATTC TCGTGCTGAC CACGGGCCTG AGCAAACCCT TCATGCGCCT GGATAAATAC
CCTACGCTGC TCAAAGAGCT CGAGAGACAC ATGGAGGATT ATCATACAGA TAGACAAGAT
ATTCAAAAAT CCATGGCTGC CTTCAAAAAC CTTTCAGCCC AATGTCAAGA AGTCCGGAAG
AGGAAAGAGC TTGAGCTGCA GATCCTGACG GAAGCCATCC GGAACTGGGA GGGCGATGAC
ATTAAAACTC TGGGCAACGT CACTTACATG TCCCAGGTCC TGATTCAGTG TGCCGGAAGT
GAGGAAAAGA ATGAAAGATA TCTTCTACTC TTCCCAAATG TTTTGCTAAT GTTGTCTGCC
AGTCCTAGGA TGAGTGGCTT TATCTATCAG GGAAAGCTTC CAACGACAGG AATGACAATC
ACAAAGCTTG AGGACAGTGA AAATCATAGA AATGCATTTG AAATATCAGG GAGCATGATT
GAGCGGATAT TAGTGTCGTG CAACAACCAG CAGGATCTGC AGGAATGGGT GGAGCACCTA
CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA AACCCCACCA TAAAGCCTCA TTCAGTGCCA
TCTCATACCC TCCCCTCCCA CCCGGTCACT CCGTCCAGCA AGCACGCAGA CAGCAAGCCC
GCGCCGCTGA CGCCCGCCTA CCACACGCTG CCCCACCCCT CCCACCACGG CACCCCGCAC
ACCACCATCA ACTGGGGACC CCTGGAGCCT CCGAAAACAC CCAAGCCCTG GAGCCTGAGC
TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC TCAGCTGCTC TCTGCTACAA GGAGGATCTT
AGTAAGAGCC CTAAGACCAT GAAAAAGCTG CTGCCCAAGC GCAAACCTGA ACGGAAGCCT
TCAGATGAGG AGTTCGCGTC CCGGAAAAGC ACAGCTGCTT TGGAAGAAGA TGCTCAGATT
CTGAAAGTCA TTGAAGCTTA CTGCACCAGC GCCAAAACAA GGCAAACACT CAATTCAAGT
TCACGCAAAG AATCTGCTCC ACAAGTTTTG CTTCCAGAAG AAGAGAAAAT TATAGTGGAA
GAAACTAAAA GTAATGGTCA GACAGTGATA GAAGAAAAGA GTCTTGTGGA TACCGTATAT
GCATTAAAGG ATGAAGTTCA AGAATTAAGA CAGGACAACA AAAAGATGAA GAAATCTCTA
GAGGAAGAAC AGAGAGCCCG CAAAGACCTG GAGAAGCTGG TGAGGAAAGT CCTGAAGAAC
ATGAATGATC CTGCCTGGGA TGAGACCAAT CTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254147.1
CDS246..2441
Misc Feature(1)306..533(+)
Misc Feature(2)528..689(+)
Misc Feature(3)543..677(+)
Misc Feature(4)780..1313(+)
Misc Feature(5)798..1313(+)
Misc Feature(6)1404..1700(+)
Translation

Target ORF information:

RefSeq Version XM_005254090
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254090

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGGACATGAC CGATAATAGC AACAATCAAC TGGTAGTAAG AGCAAAGTTT
AACTTCCAGC AGACCAATGA GGACGAGCTT TCCTTCTCAA AAGGAGACGT CATCCATGTC
ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG GGCACACTCA ACGGCCGGAC CGGCTGGTTC
CCCAGCAACT ACGTGCGCGA GGTCAAGGCC AGCGAGAAGC CTGTGTCTCC CAAATCAGGA
ACACTGAAGA GCCCTCCCAA AGGATTTGAT ACGACTGCCA TAAACAAAAG CTATTACAAT
GTGGTGCTAC AGAATATTTT AGAAACAGAA AATGAATATT CTAAAGAACT TCAGACTGTG
CTTTCAACGT ACCTACGGCC ATTGCAGACC AGTGAGAAGT TAAGTTCAGC AAACATTTCA
TATTTAATGG GAAATCTAGA AGAAATATGT TCTTTCCAGC AAATGCTCGT ACAGTCTTTA
GAAGAATGCA CCAAGTTGCC CGAAGCTCAG CAGAGAGTCG GAGGCTGCTT TTTAAACCTG
ATGCCACAGA TGAAAACCCT GTACCTCACG TATTGTGCCA ATCACCCTTC TGCAGTGAAT
GTCCTCACGG AACACAGTGA GGAGTTGGGG GAGTTCATGG AGACCAAAGG TGCCAGCAGC
CCTGGGATTC TCGTGCTGAC CACGGGCCTG AGCAAACCCT TCATGCGCCT GGATAAATAC
CCTACGCTGC TCAAAGAGCT CGAGAGACAC ATGGAGGATT ATCATACAGA TAGACAAGAT
ATTCAAAAAT CCATGGCTGC CTTCAAAAAC CTTTCAGCCC AATGTCAAGA AGTCCGGAAG
AGGAAAGAGC TTGAGCTGCA GATCCTGACG GAAGCCATCC GGAACTGGGA GGGCGATGAC
ATTAAAACTC TGGGCAACGT CACTTACATG TCCCAGGTCC TGATTCAGTG TGCCGGAAGT
GAGGAAAAGA ATGAAAGATA TCTTCTACTC TTCCCAAATG TTTTGCTAAT GTTGTCTGCC
AGTCCTAGGA TGAGTGGCTT TATCTATCAG GGAAAGCTTC CAACGACAGG AATGACAATC
ACAAAGCTTG AGGACAGTGA AAATCATAGA AATGCATTTG AAATATCAGG GAGCATGATT
GAGCGGATAT TAGTGTCGTG CAACAACCAG CAGGATCTGC AGGAATGGGT GGAGCACCTA
CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA AACCCCACCA TAAAGCCTCA TTCAGTGCCA
TCTCATACCC TCCCCTCCCA CCCGGTCACT CCGTCCAGCA AGCACGCAGA CAGCAAGCCC
GCGCCGCTGA CGCCCGCCTA CCACACGCTG CCCCACCCCT CCCACCACGG CACCCCGCAC
ACCACCATCA ACTGGGGACC CCTGGAGCCT CCGAAAACAC CCAAGCCCTG GAGCCTGAGC
TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC TCAGCTGCTC TCTGCTACAA GGAGGATCTT
AGTAAGAGCC CTAAGACCAT GAAAAAGCTG CTGCCCAAGC GCAAACCTGA ACGGAAGCCT
TCAGATGAGG AGTTCGCGTC CCGGAAAAGC ACAGCTGCTT TGGAAGAAGA TGCTCAGATT
CTGAAAGTCA TTGAAGCTTA CTGCACCAGC GCCAAAACAA GGCAAACACT CAATTCAAGT
TCACGCAAAG AATCTGCTCC ACAAGTTTTG CTTCCAGAAG AAGAGAAAAT TATAGTGGAA
GAAACTAAAA GTAATGGTCA GACAGTGATA GAAGAAAAGA GTCTTGTGGA TACCGTATAT
GCATTAAAGG ATGAAGTTCA AGAATTAAGA CAGGACAACA AAAAGATGAA GAAATCTCTA
GAGGAAGAAC AGAGAGCCCG CAAAGACCTG GAGAAGCTGG TGAGGAAAGT CCTGAAGAAC
ATGAATGATC CTGCCTGGGA TGAGACCAAT CTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47402
Clone ID Related Accession (Same CDS sequence) XM_005254091
Accession Version XM_005254091.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423444. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGAAGACGT TTGATGCAAA TGATTTGTAT CAGGGGCAGA ATTTTAACAA GGTCCTCAGT 
TCCTTAGTGA CTCTAAATAA AGTAACAGCA GACATCGGGC TGGGGAGTGA CTCCGTGTGT
GCCCGGCCCT CGTCTCACCG CATAAAGTCT TTTGACTCCC TTGGATCACA GTCTTTGCAC
ACTCGGACTT CAAAACTGTT CCAGGGCCAG TATCGGAGTT TGGACATGAC CGATAATAGC
AACAATCAAC TGGTAGTAAG AGCAAAGTTT AACTTCCAGC AGACCAATGA GGACGAGCTT
TCCTTCTCAA AAGGAGACGT CATCCATGTC ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG
GGCACACTCA ACGGCCGGAC CGGCTGGTTC CCCAGCAACT ACGTGCGCGA GGTCAAGGCC
AGCGAGAAGC CTGTGTCTCC CAAATCAGGA ACACTGAAGA GCCCTCCCAA AGGATTTGAT
ACGACTGCCA TAAACAAAAG CTATTACAAT GTGGTGCTAC AGAATATTTT AGAAACAGAA
AATGAATATT CTAAAGAACT TCAGACTGTG CTTTCAACGT ACCTACGGCC ATTGCAGACC
AGTGAGAAGT TAAGTTCAGC AAACATTTCA TATTTAATGG GAAATCTAGA AGAAATATGT
TCTTTCCAGC AAATGCTCGT ACAGTCTTTA GAAGAATGCA CCAAGTTGCC CGAAGCTCAG
CAGAGAGTCG GAGGCTGCTT TTTAAACCTG ATGCCACAGA TGAAAACCCT GTACCTCACG
TATTGTGCCA ATCACCCTTC TGCAGTGAAT GTCCTCACGG AACACAGTGA GGAGTTGGGG
GAGTTCATGG AGACCAAAGG TGCCAGCAGC CCTGGGATTC TCGTGCTGAC CACGGGCCTG
AGCAAACCCT TCATGCGCCT GGATAAATAC CCTACGCTGC TCAAAGAGCT CGAGAGACAC
ATGGAGGATT ATCATACAGA TAGACAAGAT ATTCAAAAAT CCATGGCTGC CTTCAAAAAC
CTTTCAGCCC AATGTCAAGA AGTCCGGAAG AGGAAAGAGC TTGAGCTGCA GATCCTGACG
GAAGCCATCC GGAACTGGGA GGGCGATGAC ATTAAAACTC TGGGCAACGT CACTTACATG
TCCCAGGTCC TGATTCAGTG TGCCGGAAGT GAGGAAAAGA ATGAAAGATA TCTTCTACTC
TTCCCAAATG TTTTGCTAAT GTTGTCTGCC AGTCCTAGGA TGAGTGGCTT TATCTATCAG
GGAAAGCTTC CAACGACAGG AATGACAATC ACAAAGCTTG AGGACAGTGA AAATCATAGA
AATGCATTTG AAATATCAGG GAGCATGATT GAGCGGATAT TAGTGTCGTG CAACAACCAG
CAGGATCTGC AGGAATGGGT GGAGCACCTA CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA
AACCCCACCA TAAAGCCTCA TTCAGTGCCA TCTCATACCC TCCCCTCCCA CCCGGTCACT
CCGTCCAGCA AGCACGCAGA CAGCAAGCCC GCGCCGCTGA CGCCCGCCTA CCACACGCTG
CCCCACCCCT CCCACCACGG CACCCCGCAC ACCACCATCA ACTGGGGACC CCTGGAGCCT
CCGAAAACAC CCAAGCCCTG GAGCCTGAGC TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC
TCAGCTGCTC TCTGCTACAA GGAGGATCTT AGTAAGAGCC CTAAGACCAT GAAAAAGCTG
CTGCCCAAGC GCAAACCTGA ACGGAAGCCT TCAGATGAGG AGTTCGCGTC CCGGAAAAGC
ACAGCTGCTT TGGAAGAAGA TGCTCAGATT CTGAAAGTCA TTGAAGCTTA CTGCACCAGC
GCCAAAACAA GGCAAACACT CAATTCAAGT TCACGCAAAG AATCTGCTCC ACAAGTTTTG
CTTCCAGAAG AAGAGAAAAT TATAGTGGAA GAAACTAAAA GTAATGGTCA GACAGTGATA
GAAGAAAAGA GTCTTGTGGA TACCGTATAT GCATTAAAGG ATGAAGTTCA AGAATTAAGA
CAGGACAACA AAAAGATGAA GAAATCTCTA GAGGAAGAAC AGAGAGCCCG CAAAGACCTG
GAGAAGCTGG TGAGGAAAGT CCTGAAGAAC ATGAATGATC CTGCCTGGGA TGAGACCAAT
CTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254148.1
CDS51..2216
Misc Feature(1)303..464(+)
Misc Feature(2)318..452(+)
Misc Feature(3)555..1088(+)
Misc Feature(4)573..1088(+)
Misc Feature(5)1179..1475(+)
Translation

Target ORF information:

RefSeq Version XM_005254091
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254091

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGAAGACGT TTGATGCAAA TGATTTGTAT CAGGGGCAGA ATTTTAACAA GGTCCTCAGT 
TCCTTAGTGA CTCTAAATAA AGTAACAGCA GACATCGGGC TGGGGAGTGA CTCCGTGTGT
GCCCGGCCCT CGTCTCACCG CATAAAGTCT TTTGACTCCC TTGGATCACA GTCTTTGCAC
ACTCGGACTT CAAAACTGTT CCAGGGCCAG TATCGGAGTT TGGACATGAC CGATAATAGC
AACAATCAAC TGGTAGTAAG AGCAAAGTTT AACTTCCAGC AGACCAATGA GGACGAGCTT
TCCTTCTCAA AAGGAGACGT CATCCATGTC ACCCGTGTGG AAGAGGGAGG CTGGTGGGAG
GGCACACTCA ACGGCCGGAC CGGCTGGTTC CCCAGCAACT ACGTGCGCGA GGTCAAGGCC
AGCGAGAAGC CTGTGTCTCC CAAATCAGGA ACACTGAAGA GCCCTCCCAA AGGATTTGAT
ACGACTGCCA TAAACAAAAG CTATTACAAT GTGGTGCTAC AGAATATTTT AGAAACAGAA
AATGAATATT CTAAAGAACT TCAGACTGTG CTTTCAACGT ACCTACGGCC ATTGCAGACC
AGTGAGAAGT TAAGTTCAGC AAACATTTCA TATTTAATGG GAAATCTAGA AGAAATATGT
TCTTTCCAGC AAATGCTCGT ACAGTCTTTA GAAGAATGCA CCAAGTTGCC CGAAGCTCAG
CAGAGAGTCG GAGGCTGCTT TTTAAACCTG ATGCCACAGA TGAAAACCCT GTACCTCACG
TATTGTGCCA ATCACCCTTC TGCAGTGAAT GTCCTCACGG AACACAGTGA GGAGTTGGGG
GAGTTCATGG AGACCAAAGG TGCCAGCAGC CCTGGGATTC TCGTGCTGAC CACGGGCCTG
AGCAAACCCT TCATGCGCCT GGATAAATAC CCTACGCTGC TCAAAGAGCT CGAGAGACAC
ATGGAGGATT ATCATACAGA TAGACAAGAT ATTCAAAAAT CCATGGCTGC CTTCAAAAAC
CTTTCAGCCC AATGTCAAGA AGTCCGGAAG AGGAAAGAGC TTGAGCTGCA GATCCTGACG
GAAGCCATCC GGAACTGGGA GGGCGATGAC ATTAAAACTC TGGGCAACGT CACTTACATG
TCCCAGGTCC TGATTCAGTG TGCCGGAAGT GAGGAAAAGA ATGAAAGATA TCTTCTACTC
TTCCCAAATG TTTTGCTAAT GTTGTCTGCC AGTCCTAGGA TGAGTGGCTT TATCTATCAG
GGAAAGCTTC CAACGACAGG AATGACAATC ACAAAGCTTG AGGACAGTGA AAATCATAGA
AATGCATTTG AAATATCAGG GAGCATGATT GAGCGGATAT TAGTGTCGTG CAACAACCAG
CAGGATCTGC AGGAATGGGT GGAGCACCTA CAGAAGCAAA CGAAGGTCAC GTCTGTGGGA
AACCCCACCA TAAAGCCTCA TTCAGTGCCA TCTCATACCC TCCCCTCCCA CCCGGTCACT
CCGTCCAGCA AGCACGCAGA CAGCAAGCCC GCGCCGCTGA CGCCCGCCTA CCACACGCTG
CCCCACCCCT CCCACCACGG CACCCCGCAC ACCACCATCA ACTGGGGACC CCTGGAGCCT
CCGAAAACAC CCAAGCCCTG GAGCCTGAGC TGCCTGCGGC CCGCGCCTCC CCTCCGGCCC
TCAGCTGCTC TCTGCTACAA GGAGGATCTT AGTAAGAGCC CTAAGACCAT GAAAAAGCTG
CTGCCCAAGC GCAAACCTGA ACGGAAGCCT TCAGATGAGG AGTTCGCGTC CCGGAAAAGC
ACAGCTGCTT TGGAAGAAGA TGCTCAGATT CTGAAAGTCA TTGAAGCTTA CTGCACCAGC
GCCAAAACAA GGCAAACACT CAATTCAAGT TCACGCAAAG AATCTGCTCC ACAAGTTTTG
CTTCCAGAAG AAGAGAAAAT TATAGTGGAA GAAACTAAAA GTAATGGTCA GACAGTGATA
GAAGAAAAGA GTCTTGTGGA TACCGTATAT GCATTAAAGG ATGAAGTTCA AGAATTAAGA
CAGGACAACA AAAAGATGAA GAAATCTCTA GAGGAAGAAC AGAGAGCCCG CAAAGACCTG
GAGAAGCTGG TGAGGAAAGT CCTGAAGAAC ATGAATGATC CTGCCTGGGA TGAGACCAAT
CTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47403
Clone ID Related Accession (Same CDS sequence) XM_005254092 , XM_005254093 , XM_005254094 , XM_006719958 , XM_006719959 , XM_011521132
Accession Version XM_005254092.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423446. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254149.1
CDS376..2529
Misc Feature(1)403..564(+)
Misc Feature(2)418..552(+)
Misc Feature(3)655..1188(+)
Misc Feature(4)673..1188(+)
Misc Feature(5)1279..1575(+)
Translation

Target ORF information:

RefSeq Version XM_005254092
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254092

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47403
Clone ID Related Accession (Same CDS sequence) XM_005254092 , XM_005254093 , XM_005254094 , XM_006719958 , XM_006719959 , XM_011521132
Accession Version XM_011521132.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519434.1
CDS348..2501
Misc Feature(1)375..536(+)
Misc Feature(2)390..524(+)
Misc Feature(3)627..1160(+)
Misc Feature(4)645..1160(+)
Misc Feature(5)1251..1547(+)
Translation

Target ORF information:

RefSeq Version XM_011521132
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521132

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47403
Clone ID Related Accession (Same CDS sequence) XM_005254092 , XM_005254093 , XM_005254094 , XM_006719958 , XM_006719959 , XM_011521132
Accession Version XM_006719958.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825280. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720021.1
CDS508..2661
Misc Feature(1)535..696(+)
Misc Feature(2)550..684(+)
Misc Feature(3)787..1320(+)
Misc Feature(4)805..1320(+)
Misc Feature(5)1411..1707(+)
Translation

Target ORF information:

RefSeq Version XM_006719958
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719958

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47403
Clone ID Related Accession (Same CDS sequence) XM_005254092 , XM_005254093 , XM_005254094 , XM_006719958 , XM_006719959 , XM_011521132
Accession Version XM_005254093.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423448. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254150.1
CDS385..2538
Misc Feature(1)412..573(+)
Misc Feature(2)427..561(+)
Misc Feature(3)664..1197(+)
Misc Feature(4)682..1197(+)
Misc Feature(5)1288..1584(+)
Translation

Target ORF information:

RefSeq Version XM_005254093
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254093

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47403
Clone ID Related Accession (Same CDS sequence) XM_005254092 , XM_005254093 , XM_005254094 , XM_006719958 , XM_006719959 , XM_011521132
Accession Version XM_005254094.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423450. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254151.1
CDS205..2358
Misc Feature(1)232..393(+)
Misc Feature(2)247..381(+)
Misc Feature(3)484..1017(+)
Misc Feature(4)502..1017(+)
Misc Feature(5)1108..1404(+)
Translation

Target ORF information:

RefSeq Version XM_005254094
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254094

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47403
Clone ID Related Accession (Same CDS sequence) XM_005254092 , XM_005254093 , XM_005254094 , XM_006719958 , XM_006719959 , XM_011521132
Accession Version XM_006719959.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825282. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720022.1
CDS362..2515
Misc Feature(1)389..550(+)
Misc Feature(2)404..538(+)
Misc Feature(3)641..1174(+)
Misc Feature(4)659..1174(+)
Misc Feature(5)1265..1561(+)
Translation

Target ORF information:

RefSeq Version XM_006719959
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719959

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG TGAGGTCCCT TGACCACTCA
AACAGCCAGA TGACGGATCT TAGTAAGAGC CCTAAGACCA TGAAAAAGCT GCTGCCCAAG
CGCAAACCTG AACGGAAGCC TTCAGATGAG GAGTTCGCGT CCCGGAAAAG CACAGCTGCT
TTGGAAGAAG ATGCTCAGAT TCTGAAAGTC ATTGAAGCTT ACTGCACCAG CGCCAAAACA
AGGCAAACAC TCAATTCAAC ATGGCAAGGC ACTGACCTGA TGCATAATCA CGTCTTGGCT
GATGATGACC AACCAAGCCT AGACTCCCTG GGGCGTCGCA GTAGCCTTTC TCGTTTGGAG
CCTTCAGACC TCTCGGAAGA CTCTGACTAT GACAGTATAT GGACAGCCCA TAGTTACAGA
ATGGGTTCTA CATCTCGTTC ACGCAAAGAA TCTGCTCCAC AAGTTTTGCT TCCAGAAGAA
GAGAAAATTA TAGTGGAAGA AACTAAAAGT AATGGTCAGA CAGTGATAGA AGAAAAGAGT
CTTGTGGATA CCGTATATGC ATTAAAGGAT GAAGTTCAAG AATTAAGACA GGACAACAAA
AAGATGAAGA AATCTCTAGA GGAAGAACAG AGAGCCCGCA AAGACCTGGA GAAGCTGGTG
AGGAAAGTCC TGAAGAACAT GAATGATCCT GCCTGGGATG AGACCAATCT ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47404
Clone ID Related Accession (Same CDS sequence) XM_006719960
Accession Version XM_006719960.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825284. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GTGAGGTCCC TTGACCACTC AAACAGCCAG
ATGACGGTAA TCATGTGTGC AAGTCAGAAT TTCCATTTTC TATCATGGGA AGTTACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720023.1
CDS37..2076
Misc Feature(1)97..357(+)
Misc Feature(2)256..354(+)
Misc Feature(3)535..696(+)
Misc Feature(4)550..684(+)
Misc Feature(5)787..1320(+)
Misc Feature(6)805..1320(+)
Misc Feature(7)1411..1707(+)
Translation

Target ORF information:

RefSeq Version XM_006719960
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719960

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GTGAGGTCCC TTGACCACTC AAACAGCCAG
ATGACGGTAA TCATGTGTGC AAGTCAGAAT TTCCATTTTC TATCATGGGA AGTTACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24709
Clone ID Related Accession (Same CDS sequence) NM_001113513 , NM_003899 , XM_005254095
Accession Version XM_005254095.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530423452. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAAGTTCACG CAAAGAATCT
GCTCCACAAG TTTTGCTTCC AGAAGAAGAG AAAATTATAG TGGAAGAAAC TAAAAGTAAT
GGTCAGACAG TGATAGAAGA AAAGAGTCTT GTGGATACCG TATATGCATT AAAGGATGAA
GTTCAAGAAT TAAGACAGGA CAACAAAAAG ATGAAGAAAT CTCTAGAGGA AGAACAGAGA
GCCCGCAAAG ACCTGGAGAA GCTGGTGAGG AAAGTCCTGA AGAACATGAA TGATCCTGCC
TGGGATGAGA CCAATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254152.1
CDS176..2116
Misc Feature(1)203..364(+)
Misc Feature(2)218..352(+)
Misc Feature(3)455..988(+)
Misc Feature(4)473..988(+)
Misc Feature(5)1079..1375(+)
Translation

Target ORF information:

RefSeq Version XM_005254095
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254095

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAAGTTCACG CAAAGAATCT
GCTCCACAAG TTTTGCTTCC AGAAGAAGAG AAAATTATAG TGGAAGAAAC TAAAAGTAAT
GGTCAGACAG TGATAGAAGA AAAGAGTCTT GTGGATACCG TATATGCATT AAAGGATGAA
GTTCAAGAAT TAAGACAGGA CAACAAAAAG ATGAAGAAAT CTCTAGAGGA AGAACAGAGA
GCCCGCAAAG ACCTGGAGAA GCTGGTGAGG AAAGTCCTGA AGAACATGAA TGATCCTGCC
TGGGATGAGA CCAATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62274
Clone ID Related Accession (Same CDS sequence) XM_011521133
Accession Version XM_011521133.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1515bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009952.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAG CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011519435.1
CDS37..1551
Misc Feature(1)97..357(+)
Misc Feature(2)256..354(+)
Misc Feature(3)535..696(+)
Misc Feature(4)550..684(+)
Misc Feature(5)787..1320(+)
Misc Feature(6)805..1320(+)
Misc Feature(7)1411..>1545(+)
Translation

Target ORF information:

RefSeq Version XM_011521133
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant X19, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521133

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAG CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24709
Clone ID Related Accession (Same CDS sequence) NM_001113513 , NM_003899 , XM_005254095
Accession Version NM_003899.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB104833.1, D63476.1, BC050521.1, CF144155.1, CF121796.1 and AA457036.1. On Jan 24, 2008 this sequence version replaced gi:22027526. Transcript Variant: This variant (1) differs in the 5' UTR, 3' UTR, coding region, and uses a downstream start codon, compared to variant 3. Both variants 1 and 5 encode isoform a, which has a shorter N-terminus and a longer and distinct C-terminus, compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D63476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAAGTTCACG CAAAGAATCT
GCTCCACAAG TTTTGCTTCC AGAAGAAGAG AAAATTATAG TGGAAGAAAC TAAAAGTAAT
GGTCAGACAG TGATAGAAGA AAAGAGTCTT GTGGATACCG TATATGCATT AAAGGATGAA
GTTCAAGAAT TAAGACAGGA CAACAAAAAG ATGAAGAAAT CTCTAGAGGA AGAACAGAGA
GCCCGCAAAG ACCTGGAGAA GCTGGTGAGG AAAGTCCTGA AGAACATGAA TGATCCTGCC
TGGGATGAGA CCAATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003890.1
CDS498..2438
Misc Feature(1)27..29(+)
Misc Feature(2)525..686(+)
Misc Feature(3)540..674(+)
Misc Feature(4)708..710(+)
Misc Feature(5)732..734(+)
Misc Feature(6)777..1310(+)
Misc Feature(7)795..1310(+)
Misc Feature(8)1401..1697(+)
Misc Feature(9)1515..1517(+)
Misc Feature(10)2070..2072(+)
Misc Feature(11)2073..2075(+)
Misc Feature(12)2232..2234(+)
Exon (1)1..278
Gene:ARHGEF7
Gene Synonym:
Exon (2)279..363
Gene:ARHGEF7
Gene Synonym:
Exon (3)364..494
Gene:ARHGEF7
Gene Synonym:
Exon (4)495..696
Gene:ARHGEF7
Gene Synonym:
Exon (5)697..785
Gene:ARHGEF7
Gene Synonym:
Exon (6)786..880
Gene:ARHGEF7
Gene Synonym:
Exon (7)881..976
Gene:ARHGEF7
Gene Synonym:
Exon (8)977..1099
Gene:ARHGEF7
Gene Synonym:
Exon (9)1100..1238
Gene:ARHGEF7
Gene Synonym:
Exon (10)1239..1298
Gene:ARHGEF7
Gene Synonym:
Exon (11)1299..1445
Gene:ARHGEF7
Gene Synonym:
Exon (12)1446..1532
Gene:ARHGEF7
Gene Synonym:
Exon (13)1533..1611
Gene:ARHGEF7
Gene Synonym:
Exon (14)1612..1751
Gene:ARHGEF7
Gene Synonym:
Exon (15)1752..1976
Gene:ARHGEF7
Gene Synonym:
Exon (16)1977..2070
Gene:ARHGEF7
Gene Synonym:
Exon (17)2071..2160
Gene:ARHGEF7
Gene Synonym:
Exon (18)2161..2260
Gene:ARHGEF7
Gene Synonym:
Exon (19)2261..2315
Gene:ARHGEF7
Gene Synonym:
Exon (20)2316..5059
Gene:ARHGEF7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003899
Organism Homo sapiens (human)
Definition Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003899

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAAGTTCACG CAAAGAATCT
GCTCCACAAG TTTTGCTTCC AGAAGAAGAG AAAATTATAG TGGAAGAAAC TAAAAGTAAT
GGTCAGACAG TGATAGAAGA AAAGAGTCTT GTGGATACCG TATATGCATT AAAGGATGAA
GTTCAAGAAT TAAGACAGGA CAACAAAAAG ATGAAGAAAT CTCTAGAGGA AGAACAGAGA
GCCCGCAAAG ACCTGGAGAA GCTGGTGAGG AAAGTCCTGA AGAACATGAA TGATCCTGCC
TGGGATGAGA CCAATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24860
Clone ID Related Accession (Same CDS sequence) NM_145735
Accession Version NM_145735.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC060776.1, AL834228.1, BM677392.1, DB180957.1 and AK093326.1. On Jan 24, 2008 this sequence version replaced gi:22027527. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 3. The resulting protein (isoform b) is shorter than isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB177848.1, AL834228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAACATGGC AAGGCACTGA CCTGATGCAT
AATCACGTCT TGGCTGATGA TGACCAACCA AGCCTAGACT CCCTGGGGCG TCGCAGTAGC
CTTTCTCGTT TGGAGCCTTC AGACCTCTCG GAAGACTCTG ACTATGACAG TATATGGACA
GCCCATAGTT ACAGAATGGG TTCTACATCT CGTAAGAGCT GTTGCTCATA TATCTCTCAC
CAGAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_663788.1
CDS251..2599
Misc Feature(1)311..571(+)
Misc Feature(2)470..568(+)
Misc Feature(3)749..910(+)
Misc Feature(4)764..898(+)
Misc Feature(5)932..934(+)
Misc Feature(6)956..958(+)
Misc Feature(7)1001..1534(+)
Misc Feature(8)1019..1534(+)
Misc Feature(9)1625..1921(+)
Misc Feature(10)1739..1741(+)
Misc Feature(11)2294..2296(+)
Misc Feature(12)2297..2299(+)
Exon (1)1..415
Gene:ARHGEF7
Gene Synonym:
Exon (2)416..502
Gene:ARHGEF7
Gene Synonym:
Exon (3)503..587
Gene:ARHGEF7
Gene Synonym:
Exon (4)588..718
Gene:ARHGEF7
Gene Synonym:
Exon (5)719..920
Gene:ARHGEF7
Gene Synonym:
Exon (6)921..1009
Gene:ARHGEF7
Gene Synonym:
Exon (7)1010..1104
Gene:ARHGEF7
Gene Synonym:
Exon (8)1105..1200
Gene:ARHGEF7
Gene Synonym:
Exon (9)1201..1323
Gene:ARHGEF7
Gene Synonym:
Exon (10)1324..1462
Gene:ARHGEF7
Gene Synonym:
Exon (11)1463..1522
Gene:ARHGEF7
Gene Synonym:
Exon (12)1523..1669
Gene:ARHGEF7
Gene Synonym:
Exon (13)1670..1756
Gene:ARHGEF7
Gene Synonym:
Exon (14)1757..1835
Gene:ARHGEF7
Gene Synonym:
Exon (15)1836..1975
Gene:ARHGEF7
Gene Synonym:
Exon (16)1976..2200
Gene:ARHGEF7
Gene Synonym:
Exon (17)2201..2294
Gene:ARHGEF7
Gene Synonym:
Exon (18)2295..2384
Gene:ARHGEF7
Gene Synonym:
Exon (19)2385..5462
Gene:ARHGEF7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_145735
Organism Homo sapiens (human)
Definition Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_145735

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGACGTTTGA TGCAAATGAT TTGTATCAGG GGCAGAATTT TAACAAGGTC
CTCAGTTCCT TAGTGACTCT AAATAAAGTA ACAGCAGACA TCGGGCTGGG GAGTGACTCC
GTGTGTGCCC GGCCCTCGTC TCACCGCATA AAGTCTTTTG ACTCCCTTGG ATCACAGTCT
TTGCACACTC GGACTTCAAA ACTGTTCCAG GGCCAGTATC GGAGTTTGGA CATGACCGAT
AATAGCAACA ATCAACTGGT AGTAAGAGCA AAGTTTAACT TCCAGCAGAC CAATGAGGAC
GAGCTTTCCT TCTCAAAAGG AGACGTCATC CATGTCACCC GTGTGGAAGA GGGAGGCTGG
TGGGAGGGCA CACTCAACGG CCGGACCGGC TGGTTCCCCA GCAACTACGT GCGCGAGGTC
AAGGCCAGCG AGAAGCCTGT GTCTCCCAAA TCAGGAACAC TGAAGAGCCC TCCCAAAGGA
TTTGATACGA CTGCCATAAA CAAAAGCTAT TACAATGTGG TGCTACAGAA TATTTTAGAA
ACAGAAAATG AATATTCTAA AGAACTTCAG ACTGTGCTTT CAACGTACCT ACGGCCATTG
CAGACCAGTG AGAAGTTAAG TTCAGCAAAC ATTTCATATT TAATGGGAAA TCTAGAAGAA
ATATGTTCTT TCCAGCAAAT GCTCGTACAG TCTTTAGAAG AATGCACCAA GTTGCCCGAA
GCTCAGCAGA GAGTCGGAGG CTGCTTTTTA AACCTGATGC CACAGATGAA AACCCTGTAC
CTCACGTATT GTGCCAATCA CCCTTCTGCA GTGAATGTCC TCACGGAACA CAGTGAGGAG
TTGGGGGAGT TCATGGAGAC CAAAGGTGCC AGCAGCCCTG GGATTCTCGT GCTGACCACG
GGCCTGAGCA AACCCTTCAT GCGCCTGGAT AAATACCCTA CGCTGCTCAA AGAGCTCGAG
AGACACATGG AGGATTATCA TACAGATAGA CAAGATATTC AAAAATCCAT GGCTGCCTTC
AAAAACCTTT CAGCCCAATG TCAAGAAGTC CGGAAGAGGA AAGAGCTTGA GCTGCAGATC
CTGACGGAAG CCATCCGGAA CTGGGAGGGC GATGACATTA AAACTCTGGG CAACGTCACT
TACATGTCCC AGGTCCTGAT TCAGTGTGCC GGAAGTGAGG AAAAGAATGA AAGATATCTT
CTACTCTTCC CAAATGTTTT GCTAATGTTG TCTGCCAGTC CTAGGATGAG TGGCTTTATC
TATCAGGGAA AGCTTCCAAC GACAGGAATG ACAATCACAA AGCTTGAGGA CAGTGAAAAT
CATAGAAATG CATTTGAAAT ATCAGGGAGC ATGATTGAGC GGATATTAGT GTCGTGCAAC
AACCAGCAGG ATCTGCAGGA ATGGGTGGAG CACCTACAGA AGCAAACGAA GGTCACGTCT
GTGGGAAACC CCACCATAAA GCCTCATTCA GTGCCATCTC ATACCCTCCC CTCCCACCCG
GTCACTCCGT CCAGCAAGCA CGCAGACAGC AAGCCCGCGC CGCTGACGCC CGCCTACCAC
ACGCTGCCCC ACCCCTCCCA CCACGGCACC CCGCACACCA CCATCAACTG GGGACCCCTG
GAGCCTCCGA AAACACCCAA GCCCTGGAGC CTGAGCTGCC TGCGGCCCGC GCCTCCCCTC
CGGCCCTCAG CTGCTCTCTG CTACAAGGAG GATCTTAGTA AGAGCCCTAA GACCATGAAA
AAGCTGCTGC CCAAGCGCAA ACCTGAACGG AAGCCTTCAG ATGAGGAGTT CGCGTCCCGG
AAAAGCACAG CTGCTTTGGA AGAAGATGCT CAGATTCTGA AAGTCATTGA AGCTTACTGC
ACCAGCGCCA AAACAAGGCA AACACTCAAT TCAACATGGC AAGGCACTGA CCTGATGCAT
AATCACGTCT TGGCTGATGA TGACCAACCA AGCCTAGACT CCCTGGGGCG TCGCAGTAGC
CTTTCTCGTT TGGAGCCTTC AGACCTCTCG GAAGACTCTG ACTATGACAG TATATGGACA
GCCCATAGTT ACAGAATGGG TTCTACATCT CGTAAGAGCT GTTGCTCATA TATCTCTCAC
CAGAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24807
Clone ID Related Accession (Same CDS sequence) NM_001113511
Accession Version NM_001113511.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC060776.1, BM677392.1, DB180957.1 and AK093326.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060776.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGCTCCTGTT TCCTCCTTCC CAGCCTCCTC AGCATCTGGT AACCACCATT
CTCTTGTCTG CTTCTACGTT TGATGCAAAT GATTTGTATC AGGGGCAGAA TTTTAACAAG
GTCCTCAGTT CCTTAGTGAC TCTAAATAAA GTAACAGCAG ACATCGGGCT GGGGAGTGAC
TCCGTGTGTG CCCGGCCCTC GTCTCACCGC ATAAAGTCTT TTGACTCCCT TGGATCACAG
TCTTTGCACA CTCGGACTTC AAAACTGTTC CAGGGCCAGT ATCGGAGTTT GGACATGACC
GATAATAGCA ACAATCAACT GGTAGTAAGA GCAAAGTTTA ACTTCCAGCA GACCAATGAG
GACGAGCTTT CCTTCTCAAA AGGAGACGTC ATCCATGTCA CCCGTGTGGA AGAGGGAGGC
TGGTGGGAGG GCACACTCAA CGGCCGGACC GGCTGGTTCC CCAGCAACTA CGTGCGCGAG
GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC AAATCAGGAA CACTGAAGAG CCCTCCCAAA
GGATTTGATA CGACTGCCAT AAACAAAAGC TATTACAATG TGGTGCTACA GAATATTTTA
GAAACAGAAA ATGAATATTC TAAAGAACTT CAGACTGTGC TTTCAACGTA CCTACGGCCA
TTGCAGACCA GTGAGAAGTT AAGTTCAGCA AACATTTCAT ATTTAATGGG AAATCTAGAA
GAAATATGTT CTTTCCAGCA AATGCTCGTA CAGTCTTTAG AAGAATGCAC CAAGTTGCCC
GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT TTAAACCTGA TGCCACAGAT GAAAACCCTG
TACCTCACGT ATTGTGCCAA TCACCCTTCT GCAGTGAATG TCCTCACGGA ACACAGTGAG
GAGTTGGGGG AGTTCATGGA GACCAAAGGT GCCAGCAGCC CTGGGATTCT CGTGCTGACC
ACGGGCCTGA GCAAACCCTT CATGCGCCTG GATAAATACC CTACGCTGCT CAAAGAGCTC
GAGAGACACA TGGAGGATTA TCATACAGAT AGACAAGATA TTCAAAAATC CATGGCTGCC
TTCAAAAACC TTTCAGCCCA ATGTCAAGAA GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG
ATCCTGACGG AAGCCATCCG GAACTGGGAG GGCGATGACA TTAAAACTCT GGGCAACGTC
ACTTACATGT CCCAGGTCCT GATTCAGTGT GCCGGAAGTG AGGAAAAGAA TGAAAGATAT
CTTCTACTCT TCCCAAATGT TTTGCTAATG TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT
ATCTATCAGG GAAAGCTTCC AACGACAGGA ATGACAATCA CAAAGCTTGA GGACAGTGAA
AATCATAGAA ATGCATTTGA AATATCAGGG AGCATGATTG AGCGGATATT AGTGTCGTGC
AACAACCAGC AGGATCTGCA GGAATGGGTG GAGCACCTAC AGAAGCAAAC GAAGGTCACG
TCTGTGGGAA ACCCCACCAT AAAGCCTCAT TCAGTGCCAT CTCATACCCT CCCCTCCCAC
CCGGTCACTC CGTCCAGCAA GCACGCAGAC AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC
CACACGCTGC CCCACCCCTC CCACCACGGC ACCCCGCACA CCACCATCAA CTGGGGACCC
CTGGAGCCTC CGAAAACACC CAAGCCCTGG AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC
CTCCGGCCCT CAGCTGCTCT CTGCTACAAG GAGGATCTTA GTAAGAGCCC TAAGACCATG
AAAAAGCTGC TGCCCAAGCG CAAACCTGAA CGGAAGCCTT CAGATGAGGA GTTCGCGTCC
CGGAAAAGCA CAGCTGCTTT GGAAGAAGAT GCTCAGATTC TGAAAGTCAT TGAAGCTTAC
TGCACCAGCG CCAAAACAAG GCAAACACTC AATTCAACAT GGCAAGGCAC TGACCTGATG
CATAATCACG TCTTGGCTGA TGATGACCAA CCAAGCCTAG ACTCCCTGGG GCGTCGCAGT
AGCCTTTCTC GTTTGGAGCC TTCAGACCTC TCGGAAGACT CTGACTATGA CAGTATATGG
ACAGCCCATA GTTACAGAAT GGGTTCTACA TCTCGTAAGA GCTGTTGCTC ATATATCTCT
CACCAGAACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001106983.1
CDS251..2662
Misc Feature(1)311..634(+)
Misc Feature(2)470..631(+)
Misc Feature(3)812..973(+)
Misc Feature(4)827..961(+)
Misc Feature(5)995..997(+)
Misc Feature(6)1064..1597(+)
Misc Feature(7)1082..1597(+)
Misc Feature(8)1688..1984(+)
Misc Feature(9)1802..1804(+)
Misc Feature(10)2330..2332(+)
Exon (1)1..415
Gene:ARHGEF7
Gene Synonym:
Exon (2)416..502
Gene:ARHGEF7
Gene Synonym:
Exon (3)503..565
Gene:ARHGEF7
Gene Synonym:
Exon (4)566..650
Gene:ARHGEF7
Gene Synonym:
Exon (5)651..781
Gene:ARHGEF7
Gene Synonym:
Exon (6)782..983
Gene:ARHGEF7
Gene Synonym:
Exon (7)984..1072
Gene:ARHGEF7
Gene Synonym:
Exon (8)1073..1167
Gene:ARHGEF7
Gene Synonym:
Exon (9)1168..1263
Gene:ARHGEF7
Gene Synonym:
Exon (10)1264..1386
Gene:ARHGEF7
Gene Synonym:
Exon (11)1387..1525
Gene:ARHGEF7
Gene Synonym:
Exon (12)1526..1585
Gene:ARHGEF7
Gene Synonym:
Exon (13)1586..1732
Gene:ARHGEF7
Gene Synonym:
Exon (14)1733..1819
Gene:ARHGEF7
Gene Synonym:
Exon (15)1820..1898
Gene:ARHGEF7
Gene Synonym:
Exon (16)1899..2038
Gene:ARHGEF7
Gene Synonym:
Exon (17)2039..2263
Gene:ARHGEF7
Gene Synonym:
Exon (18)2264..2357
Gene:ARHGEF7
Gene Synonym:
Exon (19)2358..2447
Gene:ARHGEF7
Gene Synonym:
Exon (20)2448..5525
Gene:ARHGEF7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001113511
Organism Homo sapiens (human)
Definition Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113511

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAGTCTA CCCCGAGCCC
CGGAGCGAGA GCGAGTGCCT GAGCAACATC CGCGAGTTCC TGCGCGGCTG CGGGGCTTCC
CTGCGGCTGG AGCTCCTGTT TCCTCCTTCC CAGCCTCCTC AGCATCTGGT AACCACCATT
CTCTTGTCTG CTTCTACGTT TGATGCAAAT GATTTGTATC AGGGGCAGAA TTTTAACAAG
GTCCTCAGTT CCTTAGTGAC TCTAAATAAA GTAACAGCAG ACATCGGGCT GGGGAGTGAC
TCCGTGTGTG CCCGGCCCTC GTCTCACCGC ATAAAGTCTT TTGACTCCCT TGGATCACAG
TCTTTGCACA CTCGGACTTC AAAACTGTTC CAGGGCCAGT ATCGGAGTTT GGACATGACC
GATAATAGCA ACAATCAACT GGTAGTAAGA GCAAAGTTTA ACTTCCAGCA GACCAATGAG
GACGAGCTTT CCTTCTCAAA AGGAGACGTC ATCCATGTCA CCCGTGTGGA AGAGGGAGGC
TGGTGGGAGG GCACACTCAA CGGCCGGACC GGCTGGTTCC CCAGCAACTA CGTGCGCGAG
GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC AAATCAGGAA CACTGAAGAG CCCTCCCAAA
GGATTTGATA CGACTGCCAT AAACAAAAGC TATTACAATG TGGTGCTACA GAATATTTTA
GAAACAGAAA ATGAATATTC TAAAGAACTT CAGACTGTGC TTTCAACGTA CCTACGGCCA
TTGCAGACCA GTGAGAAGTT AAGTTCAGCA AACATTTCAT ATTTAATGGG AAATCTAGAA
GAAATATGTT CTTTCCAGCA AATGCTCGTA CAGTCTTTAG AAGAATGCAC CAAGTTGCCC
GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT TTAAACCTGA TGCCACAGAT GAAAACCCTG
TACCTCACGT ATTGTGCCAA TCACCCTTCT GCAGTGAATG TCCTCACGGA ACACAGTGAG
GAGTTGGGGG AGTTCATGGA GACCAAAGGT GCCAGCAGCC CTGGGATTCT CGTGCTGACC
ACGGGCCTGA GCAAACCCTT CATGCGCCTG GATAAATACC CTACGCTGCT CAAAGAGCTC
GAGAGACACA TGGAGGATTA TCATACAGAT AGACAAGATA TTCAAAAATC CATGGCTGCC
TTCAAAAACC TTTCAGCCCA ATGTCAAGAA GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG
ATCCTGACGG AAGCCATCCG GAACTGGGAG GGCGATGACA TTAAAACTCT GGGCAACGTC
ACTTACATGT CCCAGGTCCT GATTCAGTGT GCCGGAAGTG AGGAAAAGAA TGAAAGATAT
CTTCTACTCT TCCCAAATGT TTTGCTAATG TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT
ATCTATCAGG GAAAGCTTCC AACGACAGGA ATGACAATCA CAAAGCTTGA GGACAGTGAA
AATCATAGAA ATGCATTTGA AATATCAGGG AGCATGATTG AGCGGATATT AGTGTCGTGC
AACAACCAGC AGGATCTGCA GGAATGGGTG GAGCACCTAC AGAAGCAAAC GAAGGTCACG
TCTGTGGGAA ACCCCACCAT AAAGCCTCAT TCAGTGCCAT CTCATACCCT CCCCTCCCAC
CCGGTCACTC CGTCCAGCAA GCACGCAGAC AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC
CACACGCTGC CCCACCCCTC CCACCACGGC ACCCCGCACA CCACCATCAA CTGGGGACCC
CTGGAGCCTC CGAAAACACC CAAGCCCTGG AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC
CTCCGGCCCT CAGCTGCTCT CTGCTACAAG GAGGATCTTA GTAAGAGCCC TAAGACCATG
AAAAAGCTGC TGCCCAAGCG CAAACCTGAA CGGAAGCCTT CAGATGAGGA GTTCGCGTCC
CGGAAAAGCA CAGCTGCTTT GGAAGAAGAT GCTCAGATTC TGAAAGTCAT TGAAGCTTAC
TGCACCAGCG CCAAAACAAG GCAAACACTC AATTCAACAT GGCAAGGCAC TGACCTGATG
CATAATCACG TCTTGGCTGA TGATGACCAA CCAAGCCTAG ACTCCCTGGG GCGTCGCAGT
AGCCTTTCTC GTTTGGAGCC TTCAGACCTC TCGGAAGACT CTGACTATGA CAGTATATGG
ACAGCCCATA GTTACAGAAT GGGTTCTACA TCTCGTAAGA GCTGTTGCTC ATATATCTCT
CACCAGAACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24900
Clone ID Related Accession (Same CDS sequence) NM_001113512
Accession Version NM_001113512.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform d
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC060776.1, BC033905.1, BM677392.1, DB180957.1 and AK093326.1. Transcript Variant: This variant (4) lacks two alternate in-frame exons, compared to variant 3. The resulting protein (isoform d) is shorter than isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033905.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAACGTT TGATGCAAAT
GATTTGTATC AGGGGCAGAA TTTTAACAAG GTCCTCAGTT CCTTAGTGAC TCTAAATAAA
GTAACAGCAG ACATCGGGCT GGGGAGTGAC TCCGTGTGTG CCCGGCCCTC GTCTCACCGC
ATAAAGTCTT TTGACTCCCT TGGATCACAG TCTTTGCACA CTCGGACTTC AAAACTGTTC
CAGGGCCAGT ATCGGAGTTT GGACATGACC GATAATAGCA ACAATCAACT GGTAGTAAGA
GCAAAGTTTA ACTTCCAGCA GACCAATGAG GACGAGCTTT CCTTCTCAAA AGGAGACGTC
ATCCATGTCA CCCGTGTGGA AGAGGGAGGC TGGTGGGAGG GCACACTCAA CGGCCGGACC
GGCTGGTTCC CCAGCAACTA CGTGCGCGAG GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC
AAATCAGGAA CACTGAAGAG CCCTCCCAAA GGATTTGATA CGACTGCCAT AAACAAAAGC
TATTACAATG TGGTGCTACA GAATATTTTA GAAACAGAAA ATGAATATTC TAAAGAACTT
CAGACTGTGC TTTCAACGTA CCTACGGCCA TTGCAGACCA GTGAGAAGTT AAGTTCAGCA
AACATTTCAT ATTTAATGGG AAATCTAGAA GAAATATGTT CTTTCCAGCA AATGCTCGTA
CAGTCTTTAG AAGAATGCAC CAAGTTGCCC GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT
TTAAACCTGA TGCCACAGAT GAAAACCCTG TACCTCACGT ATTGTGCCAA TCACCCTTCT
GCAGTGAATG TCCTCACGGA ACACAGTGAG GAGTTGGGGG AGTTCATGGA GACCAAAGGT
GCCAGCAGCC CTGGGATTCT CGTGCTGACC ACGGGCCTGA GCAAACCCTT CATGCGCCTG
GATAAATACC CTACGCTGCT CAAAGAGCTC GAGAGACACA TGGAGGATTA TCATACAGAT
AGACAAGATA TTCAAAAATC CATGGCTGCC TTCAAAAACC TTTCAGCCCA ATGTCAAGAA
GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG ATCCTGACGG AAGCCATCCG GAACTGGGAG
GGCGATGACA TTAAAACTCT GGGCAACGTC ACTTACATGT CCCAGGTCCT GATTCAGTGT
GCCGGAAGTG AGGAAAAGAA TGAAAGATAT CTTCTACTCT TCCCAAATGT TTTGCTAATG
TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT ATCTATCAGG GAAAGCTTCC AACGACAGGA
ATGACAATCA CAAAGCTTGA GGACAGTGAA AATCATAGAA ATGCATTTGA AATATCAGGG
AGCATGATTG AGCGGATATT AGTGTCGTGC AACAACCAGC AGGATCTGCA GGAATGGGTG
GAGCACCTAC AGAAGCAAAC GAAGGTCACG TCTGTGGGAA ACCCCACCAT AAAGCCTCAT
TCAGTGCCAT CTCATACCCT CCCCTCCCAC CCGGTCACTC CGTCCAGCAA GCACGCAGAC
AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC CACACGCTGC CCCACCCCTC CCACCACGGC
ACCCCGCACA CCACCATCAA CTGGGGACCC CTGGAGCCTC CGAAAACACC CAAGCCCTGG
AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC CTCCGGCCCT CAGCTGCTCT CTGCTACAAG
GAGGATCTTA GTAAGAGCCC TAAGACCATG AAAAAGCTGC TGCCCAAGCG CAAACCTGAA
CGGAAGCCTT CAGATGAGGA GTTCGCGTCC CGGAAAAGCA CAGCTGCTTT GGAAGAAGAT
GCTCAGATTC TGAAAGTCAT TGAAGCTTAC TGCACCAGCG CCAAAACAAG GCAAACACTC
AATTCAACAT GGCAAGGCAC TGACCTGATG CATAATCACG TCTTGGCTGA TGATGACCAA
CCAAGCCTAG ACTCCCTGGG GCGTCGCAGT AGCCTTTCTC GTTTGGAGCC TTCAGACCTC
TCGGAAGACT CTGACTATGA CAGTATATGG ACAGCCCATA GTTACAGAAT GGGTTCTACA
TCTCGTAAGA GCTGTTGCTC ATATATCTCT CACCAGAACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001106984.1
CDS251..2512
Misc Feature(1)311..>475(+)
Misc Feature(2)662..823(+)
Misc Feature(3)677..811(+)
Misc Feature(4)914..1447(+)
Misc Feature(5)932..1447(+)
Misc Feature(6)1538..1834(+)
Exon (1)1..415
Gene:ARHGEF7
Gene Synonym:
Exon (2)416..500
Gene:ARHGEF7
Gene Synonym:
Exon (3)501..631
Gene:ARHGEF7
Gene Synonym:
Exon (4)632..833
Gene:ARHGEF7
Gene Synonym:
Exon (5)834..922
Gene:ARHGEF7
Gene Synonym:
Exon (6)923..1017
Gene:ARHGEF7
Gene Synonym:
Exon (7)1018..1113
Gene:ARHGEF7
Gene Synonym:
Exon (8)1114..1236
Gene:ARHGEF7
Gene Synonym:
Exon (9)1237..1375
Gene:ARHGEF7
Gene Synonym:
Exon (10)1376..1435
Gene:ARHGEF7
Gene Synonym:
Exon (11)1436..1582
Gene:ARHGEF7
Gene Synonym:
Exon (12)1583..1669
Gene:ARHGEF7
Gene Synonym:
Exon (13)1670..1748
Gene:ARHGEF7
Gene Synonym:
Exon (14)1749..1888
Gene:ARHGEF7
Gene Synonym:
Exon (15)1889..2113
Gene:ARHGEF7
Gene Synonym:
Exon (16)2114..2207
Gene:ARHGEF7
Gene Synonym:
Exon (17)2208..2297
Gene:ARHGEF7
Gene Synonym:
Exon (18)2298..5375
Gene:ARHGEF7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001113512
Organism Homo sapiens (human)
Definition Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113512

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGAATTCCG CCGAGCAAAC CGTTACGTGG CTCATCACTC TGGGGGTGCT GGAGTCGCCC 
AAAAAAACCA TCTCGGACCC GGAGGGCTTT CTGCAGGCGT CGCTGAAGGA TGGGGTGGTC
CTCTGCAGGC TGCTGGAGCG CCTGCTCCCC GGGACCATCG AGAAAACGTT TGATGCAAAT
GATTTGTATC AGGGGCAGAA TTTTAACAAG GTCCTCAGTT CCTTAGTGAC TCTAAATAAA
GTAACAGCAG ACATCGGGCT GGGGAGTGAC TCCGTGTGTG CCCGGCCCTC GTCTCACCGC
ATAAAGTCTT TTGACTCCCT TGGATCACAG TCTTTGCACA CTCGGACTTC AAAACTGTTC
CAGGGCCAGT ATCGGAGTTT GGACATGACC GATAATAGCA ACAATCAACT GGTAGTAAGA
GCAAAGTTTA ACTTCCAGCA GACCAATGAG GACGAGCTTT CCTTCTCAAA AGGAGACGTC
ATCCATGTCA CCCGTGTGGA AGAGGGAGGC TGGTGGGAGG GCACACTCAA CGGCCGGACC
GGCTGGTTCC CCAGCAACTA CGTGCGCGAG GTCAAGGCCA GCGAGAAGCC TGTGTCTCCC
AAATCAGGAA CACTGAAGAG CCCTCCCAAA GGATTTGATA CGACTGCCAT AAACAAAAGC
TATTACAATG TGGTGCTACA GAATATTTTA GAAACAGAAA ATGAATATTC TAAAGAACTT
CAGACTGTGC TTTCAACGTA CCTACGGCCA TTGCAGACCA GTGAGAAGTT AAGTTCAGCA
AACATTTCAT ATTTAATGGG AAATCTAGAA GAAATATGTT CTTTCCAGCA AATGCTCGTA
CAGTCTTTAG AAGAATGCAC CAAGTTGCCC GAAGCTCAGC AGAGAGTCGG AGGCTGCTTT
TTAAACCTGA TGCCACAGAT GAAAACCCTG TACCTCACGT ATTGTGCCAA TCACCCTTCT
GCAGTGAATG TCCTCACGGA ACACAGTGAG GAGTTGGGGG AGTTCATGGA GACCAAAGGT
GCCAGCAGCC CTGGGATTCT CGTGCTGACC ACGGGCCTGA GCAAACCCTT CATGCGCCTG
GATAAATACC CTACGCTGCT CAAAGAGCTC GAGAGACACA TGGAGGATTA TCATACAGAT
AGACAAGATA TTCAAAAATC CATGGCTGCC TTCAAAAACC TTTCAGCCCA ATGTCAAGAA
GTCCGGAAGA GGAAAGAGCT TGAGCTGCAG ATCCTGACGG AAGCCATCCG GAACTGGGAG
GGCGATGACA TTAAAACTCT GGGCAACGTC ACTTACATGT CCCAGGTCCT GATTCAGTGT
GCCGGAAGTG AGGAAAAGAA TGAAAGATAT CTTCTACTCT TCCCAAATGT TTTGCTAATG
TTGTCTGCCA GTCCTAGGAT GAGTGGCTTT ATCTATCAGG GAAAGCTTCC AACGACAGGA
ATGACAATCA CAAAGCTTGA GGACAGTGAA AATCATAGAA ATGCATTTGA AATATCAGGG
AGCATGATTG AGCGGATATT AGTGTCGTGC AACAACCAGC AGGATCTGCA GGAATGGGTG
GAGCACCTAC AGAAGCAAAC GAAGGTCACG TCTGTGGGAA ACCCCACCAT AAAGCCTCAT
TCAGTGCCAT CTCATACCCT CCCCTCCCAC CCGGTCACTC CGTCCAGCAA GCACGCAGAC
AGCAAGCCCG CGCCGCTGAC GCCCGCCTAC CACACGCTGC CCCACCCCTC CCACCACGGC
ACCCCGCACA CCACCATCAA CTGGGGACCC CTGGAGCCTC CGAAAACACC CAAGCCCTGG
AGCCTGAGCT GCCTGCGGCC CGCGCCTCCC CTCCGGCCCT CAGCTGCTCT CTGCTACAAG
GAGGATCTTA GTAAGAGCCC TAAGACCATG AAAAAGCTGC TGCCCAAGCG CAAACCTGAA
CGGAAGCCTT CAGATGAGGA GTTCGCGTCC CGGAAAAGCA CAGCTGCTTT GGAAGAAGAT
GCTCAGATTC TGAAAGTCAT TGAAGCTTAC TGCACCAGCG CCAAAACAAG GCAAACACTC
AATTCAACAT GGCAAGGCAC TGACCTGATG CATAATCACG TCTTGGCTGA TGATGACCAA
CCAAGCCTAG ACTCCCTGGG GCGTCGCAGT AGCCTTTCTC GTTTGGAGCC TTCAGACCTC
TCGGAAGACT CTGACTATGA CAGTATATGG ACAGCCCATA GTTACAGAAT GGGTTCTACA
TCTCGTAAGA GCTGTTGCTC ATATATCTCT CACCAGAACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24709
Clone ID Related Accession (Same CDS sequence) NM_001113513 , NM_003899 , XM_005254095
Accession Version NM_001113513.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product rho guanine nucleotide exchange factor 7 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA216264.1, BC050521.1, D63476.1, CF144155.1, CF121796.1 and AA457036.1. Transcript Variant: This variant (1) differs in the 5' UTR, 3' UTR, coding region, and uses a downstream start codon, compared to variant 3. Both variants 1 and 5 encode isoform a, which has a shorter N-terminus and a longer and distinct C-terminus, compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAAGTTCACG CAAAGAATCT
GCTCCACAAG TTTTGCTTCC AGAAGAAGAG AAAATTATAG TGGAAGAAAC TAAAAGTAAT
GGTCAGACAG TGATAGAAGA AAAGAGTCTT GTGGATACCG TATATGCATT AAAGGATGAA
GTTCAAGAAT TAAGACAGGA CAACAAAAAG ATGAAGAAAT CTCTAGAGGA AGAACAGAGA
GCCCGCAAAG ACCTGGAGAA GCTGGTGAGG AAAGTCCTGA AGAACATGAA TGATCCTGCC
TGGGATGAGA CCAATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001106985.1
CDS385..2325
Misc Feature(1)412..573(+)
Misc Feature(2)427..561(+)
Misc Feature(3)664..1197(+)
Misc Feature(4)682..1197(+)
Misc Feature(5)1288..1584(+)
Exon (1)1..165
Gene:ARHGEF7
Gene Synonym:
Exon (2)166..250
Gene:ARHGEF7
Gene Synonym:
Exon (3)251..381
Gene:ARHGEF7
Gene Synonym:
Exon (4)382..583
Gene:ARHGEF7
Gene Synonym:
Exon (5)584..672
Gene:ARHGEF7
Gene Synonym:
Exon (6)673..767
Gene:ARHGEF7
Gene Synonym:
Exon (7)768..863
Gene:ARHGEF7
Gene Synonym:
Exon (8)864..986
Gene:ARHGEF7
Gene Synonym:
Exon (9)987..1125
Gene:ARHGEF7
Gene Synonym:
Exon (10)1126..1185
Gene:ARHGEF7
Gene Synonym:
Exon (11)1186..1332
Gene:ARHGEF7
Gene Synonym:
Exon (12)1333..1419
Gene:ARHGEF7
Gene Synonym:
Exon (13)1420..1498
Gene:ARHGEF7
Gene Synonym:
Exon (14)1499..1638
Gene:ARHGEF7
Gene Synonym:
Exon (15)1639..1863
Gene:ARHGEF7
Gene Synonym:
Exon (16)1864..1957
Gene:ARHGEF7
Gene Synonym:
Exon (17)1958..2047
Gene:ARHGEF7
Gene Synonym:
Exon (18)2048..2147
Gene:ARHGEF7
Gene Synonym:
Exon (19)2148..2202
Gene:ARHGEF7
Gene Synonym:
Exon (20)2203..4946
Gene:ARHGEF7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001113513
Organism Homo sapiens (human)
Definition Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113513

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACCGATA ATAGCAACAA TCAACTGGTA GTAAGAGCAA AGTTTAACTT CCAGCAGACC 
AATGAGGACG AGCTTTCCTT CTCAAAAGGA GACGTCATCC ATGTCACCCG TGTGGAAGAG
GGAGGCTGGT GGGAGGGCAC ACTCAACGGC CGGACCGGCT GGTTCCCCAG CAACTACGTG
CGCGAGGTCA AGGCCAGCGA GAAGCCTGTG TCTCCCAAAT CAGGAACACT GAAGAGCCCT
CCCAAAGGAT TTGATACGAC TGCCATAAAC AAAAGCTATT ACAATGTGGT GCTACAGAAT
ATTTTAGAAA CAGAAAATGA ATATTCTAAA GAACTTCAGA CTGTGCTTTC AACGTACCTA
CGGCCATTGC AGACCAGTGA GAAGTTAAGT TCAGCAAACA TTTCATATTT AATGGGAAAT
CTAGAAGAAA TATGTTCTTT CCAGCAAATG CTCGTACAGT CTTTAGAAGA ATGCACCAAG
TTGCCCGAAG CTCAGCAGAG AGTCGGAGGC TGCTTTTTAA ACCTGATGCC ACAGATGAAA
ACCCTGTACC TCACGTATTG TGCCAATCAC CCTTCTGCAG TGAATGTCCT CACGGAACAC
AGTGAGGAGT TGGGGGAGTT CATGGAGACC AAAGGTGCCA GCAGCCCTGG GATTCTCGTG
CTGACCACGG GCCTGAGCAA ACCCTTCATG CGCCTGGATA AATACCCTAC GCTGCTCAAA
GAGCTCGAGA GACACATGGA GGATTATCAT ACAGATAGAC AAGATATTCA AAAATCCATG
GCTGCCTTCA AAAACCTTTC AGCCCAATGT CAAGAAGTCC GGAAGAGGAA AGAGCTTGAG
CTGCAGATCC TGACGGAAGC CATCCGGAAC TGGGAGGGCG ATGACATTAA AACTCTGGGC
AACGTCACTT ACATGTCCCA GGTCCTGATT CAGTGTGCCG GAAGTGAGGA AAAGAATGAA
AGATATCTTC TACTCTTCCC AAATGTTTTG CTAATGTTGT CTGCCAGTCC TAGGATGAGT
GGCTTTATCT ATCAGGGAAA GCTTCCAACG ACAGGAATGA CAATCACAAA GCTTGAGGAC
AGTGAAAATC ATAGAAATGC ATTTGAAATA TCAGGGAGCA TGATTGAGCG GATATTAGTG
TCGTGCAACA ACCAGCAGGA TCTGCAGGAA TGGGTGGAGC ACCTACAGAA GCAAACGAAG
GTCACGTCTG TGGGAAACCC CACCATAAAG CCTCATTCAG TGCCATCTCA TACCCTCCCC
TCCCACCCGG TCACTCCGTC CAGCAAGCAC GCAGACAGCA AGCCCGCGCC GCTGACGCCC
GCCTACCACA CGCTGCCCCA CCCCTCCCAC CACGGCACCC CGCACACCAC CATCAACTGG
GGACCCCTGG AGCCTCCGAA AACACCCAAG CCCTGGAGCC TGAGCTGCCT GCGGCCCGCG
CCTCCCCTCC GGCCCTCAGC TGCTCTCTGC TACAAGGAGG ATCTTAGTAA GAGCCCTAAG
ACCATGAAAA AGCTGCTGCC CAAGCGCAAA CCTGAACGGA AGCCTTCAGA TGAGGAGTTC
GCGTCCCGGA AAAGCACAGC TGCTTTGGAA GAAGATGCTC AGATTCTGAA AGTCATTGAA
GCTTACTGCA CCAGCGCCAA AACAAGGCAA ACACTCAATT CAAGTTCACG CAAAGAATCT
GCTCCACAAG TTTTGCTTCC AGAAGAAGAG AAAATTATAG TGGAAGAAAC TAAAAGTAAT
GGTCAGACAG TGATAGAAGA AAAGAGTCTT GTGGATACCG TATATGCATT AAAGGATGAA
GTTCAAGAAT TAAGACAGGA CAACAAAAAG ATGAAGAAAT CTCTAGAGGA AGAACAGAGA
GCCCGCAAAG ACCTGGAGAA GCTGGTGAGG AAAGTCCTGA AGAACATGAA TGATCCTGCC
TGGGATGAGA CCAATCTATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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