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MS4A1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MS4A1
Entrez Gene ID 931
Full Name membrane spanning 4-domains A1
Synonyms B1,Bp35,CD20,CVID5,LEU-16,MS4A2,S7
General protein information
Preferred Names

membrane spanning 4-domains A1

Names

B-lymphocyte antigen CD20
B-lymphocyte cell-surface antigen B1
CD20 antigen
CD20 receptor
leukocyte surface antigen Leu-16
membrane-spanning 4-domains, subfamily A, member 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q12.2

Summary This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008].
Disorder MIM:

112210

Disorder Html: Immunodeficiency, common variable, 5, 613495 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_152866.2 NP_690605.1 B-lymphocyte antigen CD20
NM_021950.3 NP_068769.2 B-lymphocyte antigen CD20



Pan troglodytes (chimpanzee) MS4A1 XP_508461.2
Bos taurus (cattle) MS4A1 NP_001071322.1
Canis lupus familiaris (dog) MS4A1 NP_001041493.1
Mus musculus (house mouse) Ms4a1 NP_031667.1
Macaca mulatta (rhesus monkey) MS4A1 XP_001086364.1
Rattus norvegicus (Norway rat) Ms4a1 NP_001101048.1
Homo sapiens (human) MS4A1 NP_690605.1


Related articles in PubMed

Overcoming rituximab drug-resistance by the genetically engineered anti-CD20-hIFN-α fusion protein: Direct cytotoxicity and synergy with chemotherapy.
Vega GG, Franco-Cea LA, Huerta-Yepez S, Mayani H, Morrison SL, Bonavida B, Vega MI
International journal of oncology47(5)1735-48(2015 Nov)

Multispectral Imaging Approach to the Diagnosis of a CD20+ Cutaneous T-cell Lymphoproliferative Disorder: A Case Report.
Salva KA, Bennett D, Longley J, Guitart J, Wood GS
The American Journal of dermatopathology37(10)e116-21(2015 Oct)

CD20 alternative splicing isoform generates immunogenic CD4 helper T epitopes.
Vauchy C, Gamonet C, Ferrand C, Daguindau E, Galaine J, Beziaud L, Chauchet A, Henry Dunand CJ, Deschamps M, Rohrlich PS, Borg C, Adotevi O, Godet Y
International journal of cancer137(1)116-26(2015 Jul)

Establishment and Characterization of a CD20-Positive NK/T-Cell Lymphoma Cell Line.
Gu L, Hong L, Ling Z, Feng J, Zheng Z, Du L, Mou H, Sun W, Kong X, Ling Y, Jiang Z, Zhu C, Mao W, Qian L
Clinical laboratory61(7)731-9(2015)

Inhibitors of SRC kinases impair antitumor activity of anti-CD20 monoclonal antibodies.
Winiarska M, Bojarczuk K, Pyrzynska B, Bil J, Siernicka M, Dwojak M, Bobrowicz M, Miazek N, Zapala P, Zagozdzon A, Krol M, Syta A, Podszywalow-Bartnicka P, Pilch Z, Dabrowska-Iwanicka A, Juszczynski P, Efremov DG, Slabicki M, Zenz T, Le Roy A, Olive D, Rygiel TP, Leusen JH, Golab J
mAbs6(5)1300-13(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PZ-DHA also arrested MDA-MB-231 cell division at the G2/M phase and down-regulated expression of cyclin B1 and cyclin-dependent kinase 1 (CDK1)
Title: Docosahexaenoic acid-acylated phloridzin, a novel polyphenol fatty acid ester derivative, is cytotoxic to breast cancer cells.

IL-17-producing pathogenic T lymphocytes co-express CD20 in primary Sjogren's syndrome patients.
Title: Interleukin (IL)-17-producing pathogenic T lymphocytes co-express CD20 and are depleted by rituximab in primary Sjögren's syndrome: a pilot study.

Data suggest that insulitis (destruction of pancreatic beta-cells and their ability to produce/secrete insulin) occurs in two distinct profiles in type 1 diabetes; these differ in proportion of CD20-positive B-lymphocytes (relative to CD4-positive T-lymphocytes) present within the infiltrate; greater infiltration of CD20-positive B-lymphocytes leads to more aggressive disease progression.
Title: Differential Insulitic Profiles Determine the Extent of β-Cell Destruction and the Age at Onset of Type 1 Diabetes.

Data indicate that Philadelphia chromosome-positive (Ph-positive) acute lymphoblastic leukemia (ALL) patients who presented with cyclin dependent kinase inhibitor 2A/B (CDKN2A/B) deletion showed a higher rate of CD20 antigen expression.
Title: Correlation between deletion of the CDKN2 gene and tyrosine kinase inhibitor resistance in adult Philadelphia chromosome-positive acute lymphoblastic leukemia.

lipid formulations based on a polyplex or lipoplex backbone additionally equipped with anti-CD20 antibodies are promising non-viral vectors for specific oligonucleotide transfer into human tumor cells.
Title: Novel antisense therapeutics delivery systems: In vitro and in vivo studies of liposomes targeted with anti-CD20 antibody.

The following MS4A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MS4A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu00965 NM_021950.3
Latest version!
Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu00965 NM_152866.2
Latest version!
Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu00965
Clone ID Related Accession (Same CDS sequence) NM_152866.2 , NM_021950.3
Accession Version NM_152866.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 894bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-27
Organism Homo sapiens(Human)
Product B-lymphocyte antigen CD20
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD691924.1, AW504475.1, BC002807.2 and AP001034.5. This sequence is a reference standard in the RefSeqGene project. On Jun 30, 2005 this sequence version replaced NM_152866.1. Transcript Variant: This variant (1) represents the longer transcript variant. Variants 1 and 3 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.27825.1, SRR1163658.295869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACAACAC CCAGAAATTC AGTAAATGGG ACTTTCCCGG CAGAGCCAAT GAAAGGCCCT 
ATTGCTATGC AATCTGGTCC AAAACCACTC TTCAGGAGGA TGTCTTCACT GGTGGGCCCC
ACGCAAAGCT TCTTCATGAG GGAATCTAAG ACTTTGGGGG CTGTCCAGAT TATGAATGGG
CTCTTCCACA TTGCCCTGGG GGGTCTTCTG ATGATCCCAG CAGGGATCTA TGCACCCATC
TGTGTGACTG TGTGGTACCC TCTCTGGGGA GGCATTATGT ATATTATTTC CGGATCACTC
CTGGCAGCAA CGGAGAAAAA CTCCAGGAAG TGTTTGGTCA AAGGAAAAAT GATAATGAAT
TCATTGAGCC TCTTTGCTGC CATTTCTGGA ATGATTCTTT CAATCATGGA CATACTTAAT
ATTAAAATTT CCCATTTTTT AAAAATGGAG AGTCTGAATT TTATTAGAGC TCACACACCA
TATATTAACA TATACAACTG TGAACCAGCT AATCCCTCTG AGAAAAACTC CCCATCTACC
CAATACTGTT ACAGCATACA ATCTCTGTTC TTGGGCATTT TGTCAGTGAT GCTGATCTTT
GCCTTCTTCC AGGAACTTGT AATAGCTGGC ATCGTTGAGA ATGAATGGAA AAGAACGTGC
TCCAGACCCA AATCTAACAT AGTTCTCCTG TCAGCAGAAG AAAAAAAAGA ACAGACTATT
GAAATAAAAG AAGAAGTGGT TGGGCTAACT GAAACATCTT CCCAACCAAA GAATGAAGAA
GACATTGAAA TTATTCCAAT CCAAGAAGAG GAAGAAGAAG AAACAGAGAC GAACTTTCCA
GAACCTCCCC AAGATCAGGA ATCCTCACCA ATAGAAAATG ACAGCTCTCC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_690605.1
CDS417..1310
Misc Feature(1)342..344
Misc Feature(2)522..524
Misc Feature(3)567..1064
Misc Feature(4)585..650
Misc Feature(5)636..656
Misc Feature(6)669..731
Misc Feature(7)777..839
Misc Feature(8)852..896
Misc Feature(9)918..941
Misc Feature(10)981..1043
Misc Feature(11)1089..1091
Misc Feature(12)1107..1109
Misc Feature(13)1107..1109
Misc Feature(14)1131..1133
Misc Feature(15)1164..1166
Misc Feature(16)1164..1166
Misc Feature(17)1281..1283
Misc Feature(18)1281..1283
Exon (1)1..137
Exon (2)138..226
Exon (3)227..575
Exon (4)576..695
Exon (5)696..752
Exon (6)753..989
Exon (7)990..1091
Exon (8)1092..3594
Translation

Target ORF information:

RefSeq Version NM_152866.2
Organism Homo sapiens(Human)
Definition Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152866.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACAACAC CCAGAAATTC AGTAAATGGG ACTTTCCCGG CAGAGCCAAT GAAAGGCCCT 
ATTGCTATGC AATCTGGTCC AAAACCACTC TTCAGGAGGA TGTCTTCACT GGTGGGCCCC
ACGCAAAGCT TCTTCATGAG GGAATCTAAG ACTTTGGGGG CTGTCCAGAT TATGAATGGG
CTCTTCCACA TTGCCCTGGG GGGTCTTCTG ATGATCCCAG CAGGGATCTA TGCACCCATC
TGTGTGACTG TGTGGTACCC TCTCTGGGGA GGCATTATGT ATATTATTTC CGGATCACTC
CTGGCAGCAA CGGAGAAAAA CTCCAGGAAG TGTTTGGTCA AAGGAAAAAT GATAATGAAT
TCATTGAGCC TCTTTGCTGC CATTTCTGGA ATGATTCTTT CAATCATGGA CATACTTAAT
ATTAAAATTT CCCATTTTTT AAAAATGGAG AGTCTGAATT TTATTAGAGC TCACACACCA
TATATTAACA TATACAACTG TGAACCAGCT AATCCCTCTG AGAAAAACTC CCCATCTACC
CAATACTGTT ACAGCATACA ATCTCTGTTC TTGGGCATTT TGTCAGTGAT GCTGATCTTT
GCCTTCTTCC AGGAACTTGT AATAGCTGGC ATCGTTGAGA ATGAATGGAA AAGAACGTGC
TCCAGACCCA AATCTAACAT AGTTCTCCTG TCAGCAGAAG AAAAAAAAGA ACAGACTATT
GAAATAAAAG AAGAAGTGGT TGGGCTAACT GAAACATCTT CCCAACCAAA GAATGAAGAA
GACATTGAAA TTATTCCAAT CCAAGAAGAG GAAGAAGAAG AAACAGAGAC GAACTTTCCA
GAACCTCCCC AAGATCAGGA ATCCTCACCA ATAGAAAATG ACAGCTCTCC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00965
Clone ID Related Accession (Same CDS sequence) NM_152866.2 , NM_021950.3
Accession Version NM_021950.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 894bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-27
Organism Homo sapiens(Human)
Product B-lymphocyte antigen CD20
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD691924.1, BC002807.2 and AP001034.5. On Jun 30, 2005 this sequence version replaced NM_021950.2. Transcript Variant: This variant (3) lacks a portion of the 5' UTR, compared to variant 1, but encodes the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.223920.1, SRR1163658.424843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACAACAC CCAGAAATTC AGTAAATGGG ACTTTCCCGG CAGAGCCAAT GAAAGGCCCT 
ATTGCTATGC AATCTGGTCC AAAACCACTC TTCAGGAGGA TGTCTTCACT GGTGGGCCCC
ACGCAAAGCT TCTTCATGAG GGAATCTAAG ACTTTGGGGG CTGTCCAGAT TATGAATGGG
CTCTTCCACA TTGCCCTGGG GGGTCTTCTG ATGATCCCAG CAGGGATCTA TGCACCCATC
TGTGTGACTG TGTGGTACCC TCTCTGGGGA GGCATTATGT ATATTATTTC CGGATCACTC
CTGGCAGCAA CGGAGAAAAA CTCCAGGAAG TGTTTGGTCA AAGGAAAAAT GATAATGAAT
TCATTGAGCC TCTTTGCTGC CATTTCTGGA ATGATTCTTT CAATCATGGA CATACTTAAT
ATTAAAATTT CCCATTTTTT AAAAATGGAG AGTCTGAATT TTATTAGAGC TCACACACCA
TATATTAACA TATACAACTG TGAACCAGCT AATCCCTCTG AGAAAAACTC CCCATCTACC
CAATACTGTT ACAGCATACA ATCTCTGTTC TTGGGCATTT TGTCAGTGAT GCTGATCTTT
GCCTTCTTCC AGGAACTTGT AATAGCTGGC ATCGTTGAGA ATGAATGGAA AAGAACGTGC
TCCAGACCCA AATCTAACAT AGTTCTCCTG TCAGCAGAAG AAAAAAAAGA ACAGACTATT
GAAATAAAAG AAGAAGTGGT TGGGCTAACT GAAACATCTT CCCAACCAAA GAATGAAGAA
GACATTGAAA TTATTCCAAT CCAAGAAGAG GAAGAAGAAG AAACAGAGAC GAACTTTCCA
GAACCTCCCC AAGATCAGGA ATCCTCACCA ATAGAAAATG ACAGCTCTCC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_068769.2
CDS154..1047
Misc Feature(1)112..114
Misc Feature(2)259..261
Misc Feature(3)304..801
Misc Feature(4)322..387
Misc Feature(5)373..393
Misc Feature(6)406..468
Misc Feature(7)514..576
Misc Feature(8)589..633
Misc Feature(9)655..678
Misc Feature(10)718..780
Misc Feature(11)826..828
Misc Feature(12)844..846
Misc Feature(13)844..846
Misc Feature(14)868..870
Misc Feature(15)901..903
Misc Feature(16)901..903
Misc Feature(17)1018..1020
Misc Feature(18)1018..1020
Exon (1)1..137
Exon (2)138..312
Exon (3)313..432
Exon (4)433..489
Exon (5)490..726
Exon (6)727..828
Exon (7)829..3331
Translation

Target ORF information:

RefSeq Version NM_021950.3
Organism Homo sapiens(Human)
Definition Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021950.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACAACAC CCAGAAATTC AGTAAATGGG ACTTTCCCGG CAGAGCCAAT GAAAGGCCCT 
ATTGCTATGC AATCTGGTCC AAAACCACTC TTCAGGAGGA TGTCTTCACT GGTGGGCCCC
ACGCAAAGCT TCTTCATGAG GGAATCTAAG ACTTTGGGGG CTGTCCAGAT TATGAATGGG
CTCTTCCACA TTGCCCTGGG GGGTCTTCTG ATGATCCCAG CAGGGATCTA TGCACCCATC
TGTGTGACTG TGTGGTACCC TCTCTGGGGA GGCATTATGT ATATTATTTC CGGATCACTC
CTGGCAGCAA CGGAGAAAAA CTCCAGGAAG TGTTTGGTCA AAGGAAAAAT GATAATGAAT
TCATTGAGCC TCTTTGCTGC CATTTCTGGA ATGATTCTTT CAATCATGGA CATACTTAAT
ATTAAAATTT CCCATTTTTT AAAAATGGAG AGTCTGAATT TTATTAGAGC TCACACACCA
TATATTAACA TATACAACTG TGAACCAGCT AATCCCTCTG AGAAAAACTC CCCATCTACC
CAATACTGTT ACAGCATACA ATCTCTGTTC TTGGGCATTT TGTCAGTGAT GCTGATCTTT
GCCTTCTTCC AGGAACTTGT AATAGCTGGC ATCGTTGAGA ATGAATGGAA AAGAACGTGC
TCCAGACCCA AATCTAACAT AGTTCTCCTG TCAGCAGAAG AAAAAAAAGA ACAGACTATT
GAAATAAAAG AAGAAGTGGT TGGGCTAACT GAAACATCTT CCCAACCAAA GAATGAAGAA
GACATTGAAA TTATTCCAAT CCAAGAAGAG GAAGAAGAAG AAACAGAGAC GAACTTTCCA
GAACCTCCCC AAGATCAGGA ATCCTCACCA ATAGAAAATG ACAGCTCTCC TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Overcoming rituximab drug-resistance by the genetically engineered anti-CD20-hIFN-α fusion protein: Direct cytotoxicity and synergy with chemotherapy.
International journal of oncology47(5)1735-48(2015 Nov)
Vega GG,Franco-Cea LA,Huerta-Yepez S,Mayani H,Morrison SL,Bonavida B,Vega MI


book

Multispectral Imaging Approach to the Diagnosis of a CD20+ Cutaneous T-cell Lymphoproliferative Disorder: A Case Report.
The American Journal of dermatopathology37(10)e116-21(2015 Oct)
Salva KA,Bennett D,Longley J,Guitart J,Wood GS


book

CD20 alternative splicing isoform generates immunogenic CD4 helper T epitopes.
International journal of cancer137(1)116-26(2015 Jul)
Vauchy C,Gamonet C,Ferrand C,Daguindau E,Galaine J,Beziaud L,Chauchet A,Henry Dunand CJ,Deschamps M,Rohrlich PS,Borg C,Adotevi O,Godet Y


book

Establishment and Characterization of a CD20-Positive NK/T-Cell Lymphoma Cell Line.
Clinical laboratory61(7)731-9(2015)
Gu L,Hong L,Ling Z,Feng J,Zheng Z,Du L,Mou H,Sun W,Kong X,Ling Y,Jiang Z,Zhu C,Mao W,Qian L


book

Inhibitors of SRC kinases impair antitumor activity of anti-CD20 monoclonal antibodies.
mAbs6(5)1300-13(2014)
Winiarska M,Bojarczuk K,Pyrzynska B,Bil J,Siernicka M,Dwojak M,Bobrowicz M,Miazek N,Zapala P,Zagozdzon A,Krol M,Syta A,Podszywalow-Bartnicka P,Pilch Z,Dabrowska-Iwanicka A,Juszczynski P,Efremov DG,Slabicki M,Zenz T,Le Roy A,Olive D,Rygiel TP,Leusen JH,Golab J


 
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