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NPEPPS cDNA ORF clone, Homo sapiens (human)

Gene Symbol NPEPPS
Entrez Gene ID 9520
Full Name aminopeptidase puromycin sensitive
Synonyms AAP-S, MP100, PSA
General protein information
Preferred Names
puromycin-sensitive aminopeptidase
Names
puromycin-sensitive aminopeptidase
metalloproteinase MP100
cytosol alanyl aminopeptidase
NP_006301.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21

Summary This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606793


mRNA and Protein(s)

mRNA Protein Name
XM_011525496 XP_011523798 puromycin-sensitive aminopeptidase isoform X1
NM_006310 NP_006301 puromycin-sensitive aminopeptidase



Homo sapiens (human) NPEPPS NP_006301.3
Pan troglodytes (chimpanzee) NPEPPS XP_001173517.2
Macaca mulatta (Rhesus monkey) NPEPPS XP_001082709.2
Canis lupus familiaris (dog) NPEPPS XP_537659.4
Bos taurus (cattle) NPEPPS NP_001180088.1
Mus musculus (house mouse) Npepps NP_032968.2
Rattus norvegicus (Norway rat) Npepps NP_536320.1
Gallus gallus (chicken) NPEPPS XP_001234986.3
Danio rerio (zebrafish) npepps NP_001157499.1
Drosophila melanogaster (fruit fly) Psa NP_728614.1
Caenorhabditis elegans pam-1 NP_001023210.1
Arabidopsis thaliana (thale cress) APM1 NP_195035.2
Xenopus (Silurana) tropicalis (western clawed frog) npepps NP_001072690.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NPEPPS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPEPPS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu62914 XM_011525496 PREDICTED: Homo sapiens aminopeptidase puromycin sensitive (NPEPPS), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu08979 NM_006310 Homo sapiens aminopeptidase puromycin sensitive (NPEPPS), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $650.30
$929.00
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2-4 Clone 40% OFF
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu62914
Clone ID Related Accession (Same CDS sequence) XM_011525496
Accession Version XM_011525496.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product puromycin-sensitive aminopeptidase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCATCAT TTTCCATGGA TTGCTCTCCA AGTTTTTGTG TTCCTGGCCT TTGGAACCCC 
TTCATCATAT TCCGCAGCCG CTCCTCTCGC CGCCGCCTCC ACAGCCTGGG CCTCGCCGCG
ATGCCGGAGA AGAGGCCCTT CGAGCGGCTG CCTGCCGATG TCTCCCCCAT CAACTACAGC
CTTTGCCTCA AGCCCGACTT GCTGGACTTC ACCTTCGAGG GCAAGCTGGA GGCCGCCGCC
CAGGTGAGGC AGGCGACTAA TCAGATTGTG ATGAATTGTG CTGATATTGA TATTATTACA
GCTTCATATG CACCAGAAGG AGATGAAGAA ATACATGCTA CAGGATTTAA CTATCAGAAT
GAAGATGAAA AAGTCACCTT GTCTTTCCCT AGTACTCTGC AAACAGGTAC GGGAACCTTA
AAGATAGATT TTGTTGGAGA GCTGAATGAC AAAATGAAAG GTTTCTATAG AAGTAAATAT
ACTACCCCTT CTGGAGAGGT GCGCTATGCT GCTGTAACAC AGTTTGAGGC TACTGATGCC
CGAAGGGCTT TTCCTTGCTG GGATGAGCCT GCTATCAAAG CAACTTTTGA TATCTCATTG
GTTGTTCCTA AAGACAGAGT AGCTTTATCA AACATGAATG TAATTGACCG GAAACCATAC
CCTGATGATG AAAATTTAGT GGAAGTGAAG TTTGCCCGCA CACCTGTTAT GTCTACATAT
CTGGTGGCAT TTGTTGTGGG TGAATATGAC TTTGTAGAAA CAAGGTCAAA AGATGGTGTG
TGTGTCCGTG TTTACACTCC TGTTGGCAAA GCAGAGCAAG GAAAATTTGC GTTAGAGGTT
GCTGCTAAAA CCTTGCCTTT TTATAAGGAC TACTTCAATG TTCCTTATCC TCTACCTAAA
ATTGATCTCA TTGCTATTGC AGACTTTGCA GCTGGTGCCA TGGAGAACTG GGGCCTTGTT
ACTTATAGGG AGACTGCATT GCTTATTGAT CCAAAAAATT CCTGTTCTTC ATCCCGCCAG
TGGGTTGCTC TGGTTGTGGG ACATGAACTC GCCCATCAAT GGTTTGGAAA TCTTGTTACT
ATGGAATGGT GGACTCATCT TTGGTTAAAT GAAGGTTTTG CATCCTGGAT TGAATATCTG
TGTGTAGACC ACTGCTTCCC AGAGTATGAT ATTTGGACTC AGTTTGTTTC TGCTGATTAC
ACCCGTGCCC AGGAGCTTGA CGCCTTAGAT AACAGCCATC CTATTGAAGT CAGTGTGGGC
CATCCATCTG AGGTTGATGA GATATTTGAT GCTATATCAT ATAGCAAAGG TGCATCTGTC
ATCCGAATGC TGCATGACTA CATTGGGGAT AAGGACTTTA AGAAAGGAAT GAACATGTAT
TTAACCAAGT TCCAACAAAA GAATGCTGCC ACAGAGGATC TCTGGGAAAG TTTAGAAAAT
GCTAGTGGTA AACCTATAGC AGCTGTGATG AATACCTGGA CCAAACAAAT GGGATTTCCC
CTCATTTATG TGGAAGCTGA ACAGGTAGAA GATGACAGAT TATTGAGGTT GTCCCAAAAG
AAGTTCTGTG CTGGTGGGTC ATATGTTGGT GAAGATTGTC CCCAGTGGAT GGTCCCTATC
ACAATCTCTA CTAGTGAAGA CCCCAACCAG GCCAAACTAA AAATTCTAAT GGACAAGCCA
GAGATGAATG TGGTTTTGAA AAATGTCAAA CCAGACCAAT GGGTGAAGTT AAACTTAGGA
ACAGTTGGGT TTTATCGGAC CCAGTACAGC TCTGCCATGC TGGAAAGTTT ATTACCAGGC
ATTCGTGACC TTTCTCTGCC CCCTGTGGAT CGACTTGGAT TACAGAATGA CCTCTTCTCC
TTGGCTCGAG CTGGAATCAT TAGCACTGTA GAGGTTCTAA AAGTCATGGA GGCTTTTGTG
AATGAGCCCA ATTATACTGT ATGGAGCGAC CTGAGCTGTA ACCTGGGGAT TCTCTCAACT
CTCTTGTCCC ACACAGACTT CTATGAGGAA ATCCAGGAGT TTGTGAAAGA TGTCTTTTCA
CCTATAGGGG AGAGACTGGG CTGGGACCCC AAACCTGGAG AAGGTCATCT CGATGCACTC
CTGAGGGGCT TGGTTCTGGG AAAACTAGGA AAAGCAGGAC ATAAGGCAAC GTTAGAAGAA
GCCCGTCGTC GGTTTAAGGA CCACGTGGAA GGAAAACAGA TTCTCTCCGC TGATCTGAGG
AGTCCTGTCT ATCTGACTGT TTTGAAGCAT GGTGATGGCA CTACTTTAGA TATTATGTTA
AAACTTCATA AACAAGCAGA TATGCAAGAA GAGAAAAACC GAATCGAAAG AGTCCTTGGC
GCTACTCTTT TGCCTGACCT GATTCAAAAA GTCCTCACGT TTGCACTTTC AGAAGAGGTA
CGTCCACAGG ACACTGTATC GGTAATTGGT GGAGTAGCTG GAGGCAGCAA GCATGGTAGG
AAAGCTGCTT GGAAATTCAT AAAGGACAAC TGGGAAGAAC TTTATAACCG ATACCAGGGA
GGATTCTTAA TATCCAGACT AATAAAGCTA TCAGTTGAGG GATTTGCAGT TGATAAAATG
GCTGGAGAGG TTAAGGCTTT CTTCGAGAGT CACCCAGCTC CTTCAGCTGA GCGTACCATC
CAGCAGTGTT GTGAAAATAT TCTGCTGAAT GCTGCCTGGC TAAAGCGAGA TGCTGAGAGC
ATCCACCAGT ACCTCCTTCA GCGGAAGGCC TCACCACCCA CAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523798.1
CDS38..2785
Misc Feature(1)182..1348(+)
Misc Feature(2)206..1537(+)
Misc Feature(3)1079..1150(+)
Misc Feature(4)1757..2698(+)
Translation

Target ORF information:

RefSeq Version XM_011525496
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens aminopeptidase puromycin sensitive (NPEPPS), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525496

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCATCAT TTTCCATGGA TTGCTCTCCA AGTTTTTGTG TTCCTGGCCT TTGGAACCCC 
TTCATCATAT TCCGCAGCCG CTCCTCTCGC CGCCGCCTCC ACAGCCTGGG CCTCGCCGCG
ATGCCGGAGA AGAGGCCCTT CGAGCGGCTG CCTGCCGATG TCTCCCCCAT CAACTACAGC
CTTTGCCTCA AGCCCGACTT GCTGGACTTC ACCTTCGAGG GCAAGCTGGA GGCCGCCGCC
CAGGTGAGGC AGGCGACTAA TCAGATTGTG ATGAATTGTG CTGATATTGA TATTATTACA
GCTTCATATG CACCAGAAGG AGATGAAGAA ATACATGCTA CAGGATTTAA CTATCAGAAT
GAAGATGAAA AAGTCACCTT GTCTTTCCCT AGTACTCTGC AAACAGGTAC GGGAACCTTA
AAGATAGATT TTGTTGGAGA GCTGAATGAC AAAATGAAAG GTTTCTATAG AAGTAAATAT
ACTACCCCTT CTGGAGAGGT GCGCTATGCT GCTGTAACAC AGTTTGAGGC TACTGATGCC
CGAAGGGCTT TTCCTTGCTG GGATGAGCCT GCTATCAAAG CAACTTTTGA TATCTCATTG
GTTGTTCCTA AAGACAGAGT AGCTTTATCA AACATGAATG TAATTGACCG GAAACCATAC
CCTGATGATG AAAATTTAGT GGAAGTGAAG TTTGCCCGCA CACCTGTTAT GTCTACATAT
CTGGTGGCAT TTGTTGTGGG TGAATATGAC TTTGTAGAAA CAAGGTCAAA AGATGGTGTG
TGTGTCCGTG TTTACACTCC TGTTGGCAAA GCAGAGCAAG GAAAATTTGC GTTAGAGGTT
GCTGCTAAAA CCTTGCCTTT TTATAAGGAC TACTTCAATG TTCCTTATCC TCTACCTAAA
ATTGATCTCA TTGCTATTGC AGACTTTGCA GCTGGTGCCA TGGAGAACTG GGGCCTTGTT
ACTTATAGGG AGACTGCATT GCTTATTGAT CCAAAAAATT CCTGTTCTTC ATCCCGCCAG
TGGGTTGCTC TGGTTGTGGG ACATGAACTC GCCCATCAAT GGTTTGGAAA TCTTGTTACT
ATGGAATGGT GGACTCATCT TTGGTTAAAT GAAGGTTTTG CATCCTGGAT TGAATATCTG
TGTGTAGACC ACTGCTTCCC AGAGTATGAT ATTTGGACTC AGTTTGTTTC TGCTGATTAC
ACCCGTGCCC AGGAGCTTGA CGCCTTAGAT AACAGCCATC CTATTGAAGT CAGTGTGGGC
CATCCATCTG AGGTTGATGA GATATTTGAT GCTATATCAT ATAGCAAAGG TGCATCTGTC
ATCCGAATGC TGCATGACTA CATTGGGGAT AAGGACTTTA AGAAAGGAAT GAACATGTAT
TTAACCAAGT TCCAACAAAA GAATGCTGCC ACAGAGGATC TCTGGGAAAG TTTAGAAAAT
GCTAGTGGTA AACCTATAGC AGCTGTGATG AATACCTGGA CCAAACAAAT GGGATTTCCC
CTCATTTATG TGGAAGCTGA ACAGGTAGAA GATGACAGAT TATTGAGGTT GTCCCAAAAG
AAGTTCTGTG CTGGTGGGTC ATATGTTGGT GAAGATTGTC CCCAGTGGAT GGTCCCTATC
ACAATCTCTA CTAGTGAAGA CCCCAACCAG GCCAAACTAA AAATTCTAAT GGACAAGCCA
GAGATGAATG TGGTTTTGAA AAATGTCAAA CCAGACCAAT GGGTGAAGTT AAACTTAGGA
ACAGTTGGGT TTTATCGGAC CCAGTACAGC TCTGCCATGC TGGAAAGTTT ATTACCAGGC
ATTCGTGACC TTTCTCTGCC CCCTGTGGAT CGACTTGGAT TACAGAATGA CCTCTTCTCC
TTGGCTCGAG CTGGAATCAT TAGCACTGTA GAGGTTCTAA AAGTCATGGA GGCTTTTGTG
AATGAGCCCA ATTATACTGT ATGGAGCGAC CTGAGCTGTA ACCTGGGGAT TCTCTCAACT
CTCTTGTCCC ACACAGACTT CTATGAGGAA ATCCAGGAGT TTGTGAAAGA TGTCTTTTCA
CCTATAGGGG AGAGACTGGG CTGGGACCCC AAACCTGGAG AAGGTCATCT CGATGCACTC
CTGAGGGGCT TGGTTCTGGG AAAACTAGGA AAAGCAGGAC ATAAGGCAAC GTTAGAAGAA
GCCCGTCGTC GGTTTAAGGA CCACGTGGAA GGAAAACAGA TTCTCTCCGC TGATCTGAGG
AGTCCTGTCT ATCTGACTGT TTTGAAGCAT GGTGATGGCA CTACTTTAGA TATTATGTTA
AAACTTCATA AACAAGCAGA TATGCAAGAA GAGAAAAACC GAATCGAAAG AGTCCTTGGC
GCTACTCTTT TGCCTGACCT GATTCAAAAA GTCCTCACGT TTGCACTTTC AGAAGAGGTA
CGTCCACAGG ACACTGTATC GGTAATTGGT GGAGTAGCTG GAGGCAGCAA GCATGGTAGG
AAAGCTGCTT GGAAATTCAT AAAGGACAAC TGGGAAGAAC TTTATAACCG ATACCAGGGA
GGATTCTTAA TATCCAGACT AATAAAGCTA TCAGTTGAGG GATTTGCAGT TGATAAAATG
GCTGGAGAGG TTAAGGCTTT CTTCGAGAGT CACCCAGCTC CTTCAGCTGA GCGTACCATC
CAGCAGTGTT GTGAAAATAT TCTGCTGAAT GCTGCCTGGC TAAAGCGAGA TGCTGAGAGC
ATCCACCAGT ACCTCCTTCA GCGGAAGGCC TCACCACCCA CAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu08979
Clone ID Related Accession (Same CDS sequence) NM_006310
Accession Version NM_006310.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product puromycin-sensitive aminopeptidase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB476543.3, BC065294.1, AJ132583.1 and BM723238.1. On Oct 31, 2007 this sequence version replaced gi:15451906. CCDS Note: Experimental evidence (N-terminal protein sequencing; PubMed ID 10978616) shows a peptide starting at Pro-46, suggesting that the downstream ATG (coding for Met-45) is used. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ132583.1, BC065294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTGGCTGG CAGCTGCCGC CCCCTCCCTC GCTCGCCGCC TGCTCTTCCT CGGCCCTCCG 
CCTCCTCCCC TCCTCCTTCT CGTCTTCAGC CGCTCCTCTC GCCGCCGCCT CCACAGCCTG
GGCCTCGCCG CGATGCCGGA GAAGAGGCCC TTCGAGCGGC TGCCTGCCGA TGTCTCCCCC
ATCAACTACA GCCTTTGCCT CAAGCCCGAC TTGCTGGACT TCACCTTCGA GGGCAAGCTG
GAGGCCGCCG CCCAGGTGAG GCAGGCGACT AATCAGATTG TGATGAATTG TGCTGATATT
GATATTATTA CAGCTTCATA TGCACCAGAA GGAGATGAAG AAATACATGC TACAGGATTT
AACTATCAGA ATGAAGATGA AAAAGTCACC TTGTCTTTCC CTAGTACTCT GCAAACAGGT
ACGGGAACCT TAAAGATAGA TTTTGTTGGA GAGCTGAATG ACAAAATGAA AGGTTTCTAT
AGAAGTAAAT ATACTACCCC TTCTGGAGAG GTGCGCTATG CTGCTGTAAC ACAGTTTGAG
GCTACTGATG CCCGAAGGGC TTTTCCTTGC TGGGATGAGC CTGCTATCAA AGCAACTTTT
GATATCTCAT TGGTTGTTCC TAAAGACAGA GTAGCTTTAT CAAACATGAA TGTAATTGAC
CGGAAACCAT ACCCTGATGA TGAAAATTTA GTGGAAGTGA AGTTTGCCCG CACACCTGTT
ATGTCTACAT ATCTGGTGGC ATTTGTTGTG GGTGAATATG ACTTTGTAGA AACAAGGTCA
AAAGATGGTG TGTGTGTCCG TGTTTACACT CCTGTTGGCA AAGCAGAGCA AGGAAAATTT
GCGTTAGAGG TTGCTGCTAA AACCTTGCCT TTTTATAAGG ACTACTTCAA TGTTCCTTAT
CCTCTACCTA AAATTGATCT CATTGCTATT GCAGACTTTG CAGCTGGTGC CATGGAGAAC
TGGGGCCTTG TTACTTATAG GGAGACTGCA TTGCTTATTG ATCCAAAAAA TTCCTGTTCT
TCATCCCGCC AGTGGGTTGC TCTGGTTGTG GGACATGAAC TCGCCCATCA ATGGTTTGGA
AATCTTGTTA CTATGGAATG GTGGACTCAT CTTTGGTTAA ATGAAGGTTT TGCATCCTGG
ATTGAATATC TGTGTGTAGA CCACTGCTTC CCAGAGTATG ATATTTGGAC TCAGTTTGTT
TCTGCTGATT ACACCCGTGC CCAGGAGCTT GACGCCTTAG ATAACAGCCA TCCTATTGAA
GTCAGTGTGG GCCATCCATC TGAGGTTGAT GAGATATTTG ATGCTATATC ATATAGCAAA
GGTGCATCTG TCATCCGAAT GCTGCATGAC TACATTGGGG ATAAGGACTT TAAGAAAGGA
ATGAACATGT ATTTAACCAA GTTCCAACAA AAGAATGCTG CCACAGAGGA TCTCTGGGAA
AGTTTAGAAA ATGCTAGTGG TAAACCTATA GCAGCTGTGA TGAATACCTG GACCAAACAA
ATGGGATTTC CCCTCATTTA TGTGGAAGCT GAACAGGTAG AAGATGACAG ATTATTGAGG
TTGTCCCAAA AGAAGTTCTG TGCTGGTGGG TCATATGTTG GTGAAGATTG TCCCCAGTGG
ATGGTCCCTA TCACAATCTC TACTAGTGAA GACCCCAACC AGGCCAAACT AAAAATTCTA
ATGGACAAGC CAGAGATGAA TGTGGTTTTG AAAAATGTCA AACCAGACCA ATGGGTGAAG
TTAAACTTAG GAACAGTTGG GTTTTATCGG ACCCAGTACA GCTCTGCCAT GCTGGAAAGT
TTATTACCAG GCATTCGTGA CCTTTCTCTG CCCCCTGTGG ATCGACTTGG ATTACAGAAT
GACCTCTTCT CCTTGGCTCG AGCTGGAATC ATTAGCACTG TAGAGGTTCT AAAAGTCATG
GAGGCTTTTG TGAATGAGCC CAATTATACT GTATGGAGCG ACCTGAGCTG TAACCTGGGG
ATTCTCTCAA CTCTCTTGTC CCACACAGAC TTCTATGAGG AAATCCAGGA GTTTGTGAAA
GATGTCTTTT CACCTATAGG GGAGAGACTG GGCTGGGACC CCAAACCTGG AGAAGGTCAT
CTCGATGCAC TCCTGAGGGG CTTGGTTCTG GGAAAACTAG GAAAAGCAGG ACATAAGGCA
ACGTTAGAAG AAGCCCGTCG TCGGTTTAAG GACCACGTGG AAGGAAAACA GATTCTCTCC
GCTGATCTGA GGAGTCCTGT CTATCTGACT GTTTTGAAGC ATGGTGATGG CACTACTTTA
GATATTATGT TAAAACTTCA TAAACAAGCA GATATGCAAG AAGAGAAAAA CCGAATCGAA
AGAGTCCTTG GCGCTACTCT TTTGCCTGAC CTGATTCAAA AAGTCCTCAC GTTTGCACTT
TCAGAAGAGG TACGTCCACA GGACACTGTA TCGGTAATTG GTGGAGTAGC TGGAGGCAGC
AAGCATGGTA GGAAAGCTGC TTGGAAATTC ATAAAGGACA ACTGGGAAGA ACTTTATAAC
CGATACCAGG GAGGATTCTT AATATCCAGA CTAATAAAGC TATCAGTTGA GGGATTTGCA
GTTGATAAAA TGGCTGGAGA GGTTAAGGCT TTCTTCGAGA GTCACCCAGC TCCTTCAGCT
GAGCGTACCA TCCAGCAGTG TTGTGAAAAT ATTCTGCTGA ATGCTGCCTG GCTAAAGCGA
GATGCTGAGA GCATCCACCA GTACCTCCTT CAGCGGAAGG CCTCACCACC CACAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006301.3
CDS224..2983
Misc Feature(1)380..1546(+)
Misc Feature(2)404..1735(+)
Misc Feature(3)1169..1183(+)
Misc Feature(4)1277..1348(+)
Misc Feature(5)1535..1537(+)
Misc Feature(6)1955..2896(+)
Misc Feature(7)2399..2413(+)
Exon (1)1..478
Gene:NPEPPS
Gene Synonym:
Exon (2)479..563
Gene:NPEPPS
Gene Synonym:
Exon (3)564..641
Gene:NPEPPS
Gene Synonym:
Exon (4)642..763
Gene:NPEPPS
Gene Synonym:
Exon (5)764..871
Gene:NPEPPS
Gene Synonym:
Exon (6)872..1072
Gene:NPEPPS
Gene Synonym:
Exon (7)1073..1169
Gene:NPEPPS
Gene Synonym:
Exon (8)1170..1203
Gene:NPEPPS
Gene Synonym:
Exon (9)1204..1318
Gene:NPEPPS
Gene Synonym:
Exon (10)1319..1483
Gene:NPEPPS
Gene Synonym:
Exon (11)1484..1588
Gene:NPEPPS
Gene Synonym:
Exon (12)1589..1649
Gene:NPEPPS
Gene Synonym:
Exon (13)1650..1759
Gene:NPEPPS
Gene Synonym:
Exon (14)1760..1823
Gene:NPEPPS
Gene Synonym:
Exon (15)1824..1963
Gene:NPEPPS
Gene Synonym:
Exon (16)1964..2098
Gene:NPEPPS
Gene Synonym:
Exon (17)2099..2318
Gene:NPEPPS
Gene Synonym:
Exon (18)2319..2461
Gene:NPEPPS
Gene Synonym:
Exon (19)2462..2518
Gene:NPEPPS
Gene Synonym:
Exon (20)2519..2626
Gene:NPEPPS
Gene Synonym:
Exon (21)2627..2782
Gene:NPEPPS
Gene Synonym:
Exon (22)2783..2830
Gene:NPEPPS
Gene Synonym:
Exon (23)2831..4339
Gene:NPEPPS
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006310
Organism Homo sapiens (human)
Definition Homo sapiens aminopeptidase puromycin sensitive (NPEPPS), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006310

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGTGGCTGG CAGCTGCCGC CCCCTCCCTC GCTCGCCGCC TGCTCTTCCT CGGCCCTCCG 
CCTCCTCCCC TCCTCCTTCT CGTCTTCAGC CGCTCCTCTC GCCGCCGCCT CCACAGCCTG
GGCCTCGCCG CGATGCCGGA GAAGAGGCCC TTCGAGCGGC TGCCTGCCGA TGTCTCCCCC
ATCAACTACA GCCTTTGCCT CAAGCCCGAC TTGCTGGACT TCACCTTCGA GGGCAAGCTG
GAGGCCGCCG CCCAGGTGAG GCAGGCGACT AATCAGATTG TGATGAATTG TGCTGATATT
GATATTATTA CAGCTTCATA TGCACCAGAA GGAGATGAAG AAATACATGC TACAGGATTT
AACTATCAGA ATGAAGATGA AAAAGTCACC TTGTCTTTCC CTAGTACTCT GCAAACAGGT
ACGGGAACCT TAAAGATAGA TTTTGTTGGA GAGCTGAATG ACAAAATGAA AGGTTTCTAT
AGAAGTAAAT ATACTACCCC TTCTGGAGAG GTGCGCTATG CTGCTGTAAC ACAGTTTGAG
GCTACTGATG CCCGAAGGGC TTTTCCTTGC TGGGATGAGC CTGCTATCAA AGCAACTTTT
GATATCTCAT TGGTTGTTCC TAAAGACAGA GTAGCTTTAT CAAACATGAA TGTAATTGAC
CGGAAACCAT ACCCTGATGA TGAAAATTTA GTGGAAGTGA AGTTTGCCCG CACACCTGTT
ATGTCTACAT ATCTGGTGGC ATTTGTTGTG GGTGAATATG ACTTTGTAGA AACAAGGTCA
AAAGATGGTG TGTGTGTCCG TGTTTACACT CCTGTTGGCA AAGCAGAGCA AGGAAAATTT
GCGTTAGAGG TTGCTGCTAA AACCTTGCCT TTTTATAAGG ACTACTTCAA TGTTCCTTAT
CCTCTACCTA AAATTGATCT CATTGCTATT GCAGACTTTG CAGCTGGTGC CATGGAGAAC
TGGGGCCTTG TTACTTATAG GGAGACTGCA TTGCTTATTG ATCCAAAAAA TTCCTGTTCT
TCATCCCGCC AGTGGGTTGC TCTGGTTGTG GGACATGAAC TCGCCCATCA ATGGTTTGGA
AATCTTGTTA CTATGGAATG GTGGACTCAT CTTTGGTTAA ATGAAGGTTT TGCATCCTGG
ATTGAATATC TGTGTGTAGA CCACTGCTTC CCAGAGTATG ATATTTGGAC TCAGTTTGTT
TCTGCTGATT ACACCCGTGC CCAGGAGCTT GACGCCTTAG ATAACAGCCA TCCTATTGAA
GTCAGTGTGG GCCATCCATC TGAGGTTGAT GAGATATTTG ATGCTATATC ATATAGCAAA
GGTGCATCTG TCATCCGAAT GCTGCATGAC TACATTGGGG ATAAGGACTT TAAGAAAGGA
ATGAACATGT ATTTAACCAA GTTCCAACAA AAGAATGCTG CCACAGAGGA TCTCTGGGAA
AGTTTAGAAA ATGCTAGTGG TAAACCTATA GCAGCTGTGA TGAATACCTG GACCAAACAA
ATGGGATTTC CCCTCATTTA TGTGGAAGCT GAACAGGTAG AAGATGACAG ATTATTGAGG
TTGTCCCAAA AGAAGTTCTG TGCTGGTGGG TCATATGTTG GTGAAGATTG TCCCCAGTGG
ATGGTCCCTA TCACAATCTC TACTAGTGAA GACCCCAACC AGGCCAAACT AAAAATTCTA
ATGGACAAGC CAGAGATGAA TGTGGTTTTG AAAAATGTCA AACCAGACCA ATGGGTGAAG
TTAAACTTAG GAACAGTTGG GTTTTATCGG ACCCAGTACA GCTCTGCCAT GCTGGAAAGT
TTATTACCAG GCATTCGTGA CCTTTCTCTG CCCCCTGTGG ATCGACTTGG ATTACAGAAT
GACCTCTTCT CCTTGGCTCG AGCTGGAATC ATTAGCACTG TAGAGGTTCT AAAAGTCATG
GAGGCTTTTG TGAATGAGCC CAATTATACT GTATGGAGCG ACCTGAGCTG TAACCTGGGG
ATTCTCTCAA CTCTCTTGTC CCACACAGAC TTCTATGAGG AAATCCAGGA GTTTGTGAAA
GATGTCTTTT CACCTATAGG GGAGAGACTG GGCTGGGACC CCAAACCTGG AGAAGGTCAT
CTCGATGCAC TCCTGAGGGG CTTGGTTCTG GGAAAACTAG GAAAAGCAGG ACATAAGGCA
ACGTTAGAAG AAGCCCGTCG TCGGTTTAAG GACCACGTGG AAGGAAAACA GATTCTCTCC
GCTGATCTGA GGAGTCCTGT CTATCTGACT GTTTTGAAGC ATGGTGATGG CACTACTTTA
GATATTATGT TAAAACTTCA TAAACAAGCA GATATGCAAG AAGAGAAAAA CCGAATCGAA
AGAGTCCTTG GCGCTACTCT TTTGCCTGAC CTGATTCAAA AAGTCCTCAC GTTTGCACTT
TCAGAAGAGG TACGTCCACA GGACACTGTA TCGGTAATTG GTGGAGTAGC TGGAGGCAGC
AAGCATGGTA GGAAAGCTGC TTGGAAATTC ATAAAGGACA ACTGGGAAGA ACTTTATAAC
CGATACCAGG GAGGATTCTT AATATCCAGA CTAATAAAGC TATCAGTTGA GGGATTTGCA
GTTGATAAAA TGGCTGGAGA GGTTAAGGCT TTCTTCGAGA GTCACCCAGC TCCTTCAGCT
GAGCGTACCA TCCAGCAGTG TTGTGAAAAT ATTCTGCTGA ATGCTGCCTG GCTAAAGCGA
GATGCTGAGA GCATCCACCA GTACCTCCTT CAGCGGAAGG CCTCACCACC CACAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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