RAPGEF2 cDNA ORF clone, Homo sapiens(human)

The following RAPGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09032 NM_014247.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu95558 NM_001351726.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu95558 NM_001351725.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu95559 NM_001351727.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu105605 XM_024454286.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu105605 XM_024454287.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu95557 XM_017008856.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu95558 XM_017008858.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu95558 XM_017008857.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu95559 XM_017008859.1
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu39237 NM_001351724.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu63163 NM_001351728.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu39234 XM_005263358.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu39235 XM_006714420.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu39236 XM_005263359.4
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu39237 XM_006714421.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu39238 XM_005263360.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu39239 XM_005263361.3
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu39240 XM_006714422.4
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu39240 XM_011532426.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu63162 XM_011532425.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu63163 XM_011532427.2
Latest version!
Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OHu39236 XM_005263359.3 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu63163 NM_001351728.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu39237 NM_001351724.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu09032 NM_014247.2 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu39240 XM_006714422.3 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu95559 NM_001351727.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu95557 XM_017008856.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu09032
    Clone ID Related Accession (Same CDS sequence) NM_014247.2 , NM_014247.3
    Accession Version NM_014247.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-17
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA188103.1, AB002311.1, BU620827.1, BC110355.1 and BP386101.1. On or before Jul 25, 2007 this sequence version replaced XM_934717.2, XM_934718.2, XM_376350.4, XM_934714.2, XM_944412.2, XM_944410.2, XM_944403.2, NM_014247.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002311.1, SRR1803613.248869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055062.1
    CDS311..4810
    Translation

    Target ORF information:

    RefSeq Version NM_014247.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09032
    Clone ID Related Accession (Same CDS sequence) NM_014247.2 , NM_014247.3
    Accession Version NM_014247.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5, DA188103.1, AB002311.1, BU620827.1, BC110355.1, BP386101.1 and AC074344.5. On Jun 2, 2019 this sequence version replaced NM_014247.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002311.1, SRR1803613.248869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055062.1
    CDS348..4847
    Translation

    Target ORF information:

    RefSeq Version NM_014247.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014247.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95558
    Clone ID Related Accession (Same CDS sequence) XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
    Accession Version NM_001351726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_017008858.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.26208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338655.1
    CDS183..4817
    Translation

    Target ORF information:

    RefSeq Version NM_001351726.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95558
    Clone ID Related Accession (Same CDS sequence) XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
    Accession Version NM_001351725.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-25
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_017008857.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.104810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338654.1
    CDS1116..5750
    Translation

    Target ORF information:

    RefSeq Version NM_001351725.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95559
    Clone ID Related Accession (Same CDS sequence) NM_001351727.1 , NM_001351727.2 , XM_017008859.1
    Accession Version NM_001351727.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_017008859.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.73881.1, SRR1803617.77819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338656.1
    CDS478..5052
    Translation

    Target ORF information:

    RefSeq Version NM_001351727.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95559
    Clone ID Related Accession (Same CDS sequence) NM_001351727.1 , NM_001351727.2 , XM_017008859.1
    Accession Version NM_001351727.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On Jun 2, 2019 this sequence version replaced NM_001351727.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.77819.1, SRR1803617.73881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338656.1
    CDS348..4922
    Translation

    Target ORF information:

    RefSeq Version NM_001351727.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351727.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105605
    Clone ID Related Accession (Same CDS sequence) XM_024454286.1 , XM_024454287.1
    Accession Version XM_024454286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT
    CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT
    TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT
    ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA
    GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG
    ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT
    GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG
    TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC
    ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG
    GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC
    TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC
    CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC
    ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT
    CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG
    CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC
    TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT
    GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC
    AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG
    GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA
    AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA
    CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA
    GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT
    GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC
    ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA
    AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC
    ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA
    AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG
    ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC
    AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC
    TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT
    ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC
    ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT
    GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA
    GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG
    AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT
    GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT
    ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA
    GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG
    CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC
    AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA
    CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA
    GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT
    CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC
    GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA
    AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC
    AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA
    ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT
    CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT
    TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG
    CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC
    TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG
    CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT
    TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA
    CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA
    CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG
    TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT
    GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024310054.1
    CDS2168..6907
    Translation

    Target ORF information:

    RefSeq Version XM_024454286.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024454286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT
    CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT
    TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT
    ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA
    GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG
    ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT
    GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG
    TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC
    ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG
    GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC
    TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC
    CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC
    ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT
    CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG
    CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC
    TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT
    GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC
    AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG
    GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA
    AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA
    CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA
    GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT
    GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC
    ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA
    AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC
    ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA
    AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG
    ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC
    AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC
    TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT
    ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC
    ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT
    GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA
    GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG
    AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT
    GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT
    ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA
    GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG
    CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC
    AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA
    CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA
    GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT
    CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC
    GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA
    AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC
    AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA
    ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT
    CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT
    TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG
    CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC
    TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG
    CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT
    TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA
    CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA
    CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG
    TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT
    GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105605
    Clone ID Related Accession (Same CDS sequence) XM_024454286.1 , XM_024454287.1
    Accession Version XM_024454287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT
    CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT
    TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT
    ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA
    GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG
    ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT
    GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG
    TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC
    ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG
    GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC
    TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC
    CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC
    ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT
    CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG
    CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC
    TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT
    GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC
    AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG
    GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA
    AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA
    CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA
    GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT
    GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC
    ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA
    AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC
    ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA
    AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG
    ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC
    AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC
    TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT
    ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC
    ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT
    GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA
    GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG
    AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT
    GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT
    ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA
    GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG
    CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC
    AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA
    CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA
    GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT
    CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC
    GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA
    AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC
    AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA
    ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT
    CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT
    TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG
    CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC
    TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG
    CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT
    TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA
    CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA
    CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG
    TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT
    GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024310055.1
    CDS1210..5949
    Translation

    Target ORF information:

    RefSeq Version XM_024454287.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024454287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT
    CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT
    TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT
    ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA
    GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG
    ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT
    GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG
    TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC
    ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG
    GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC
    TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC
    CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC
    ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT
    CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG
    CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC
    TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT
    GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC
    AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG
    GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA
    AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA
    CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA
    GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT
    GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC
    ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA
    AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC
    ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA
    AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG
    ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC
    AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC
    TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT
    ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC
    ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT
    GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA
    GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG
    AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT
    GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT
    GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT
    ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA
    GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG
    CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC
    AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA
    CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA
    GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT
    CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC
    GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA
    AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC
    AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA
    ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT
    CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT
    TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG
    CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC
    TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG
    CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT
    TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA
    CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA
    CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA
    CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG
    TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT
    GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT
    TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT
    GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG
    GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT
    AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA
    ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT
    GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA
    CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT
    TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT
    TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC
    CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC
    ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC
    CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG
    GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG
    CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT
    CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA
    TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC
    AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC
    TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95557
    Clone ID Related Accession (Same CDS sequence) XM_017008856.1 , XM_017008856.2
    Accession Version XM_017008856.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA
    ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG
    CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC
    AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA
    ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG
    GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG
    AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA
    AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT
    TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT
    AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG
    TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG
    GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA
    GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG
    CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT
    GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC
    TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT
    TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC
    ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864345.1
    CDS96..5054
    Translation

    Target ORF information:

    RefSeq Version XM_017008856.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA
    ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG
    CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC
    AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA
    ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG
    GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG
    AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA
    AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT
    TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT
    AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG
    TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG
    GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA
    GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG
    CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT
    GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC
    TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT
    TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC
    ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95557
    Clone ID Related Accession (Same CDS sequence) XM_017008856.1 , XM_017008856.2
    Accession Version XM_017008856.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA
    ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG
    CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC
    AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA
    ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG
    GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG
    AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA
    AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT
    TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT
    AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG
    TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG
    GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA
    GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG
    CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT
    GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC
    TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT
    TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC
    ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864345.1
    CDS95..5053
    Translation

    Target ORF information:

    RefSeq Version XM_017008856.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA
    ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG
    CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC
    AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA
    ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG
    GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG
    AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA
    AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT
    TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT
    AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG
    TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG
    GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA
    GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG
    CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT
    GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC
    TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT
    TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG
    TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC
    ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95558
    Clone ID Related Accession (Same CDS sequence) XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
    Accession Version XM_017008857.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product rap guanine nucleotide exchange factor 2 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864346.1
    CDS1209..5843
    Translation

    Target ORF information:

    RefSeq Version XM_017008857.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95558
    Clone ID Related Accession (Same CDS sequence) XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
    Accession Version XM_017008858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product rap guanine nucleotide exchange factor 2 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864347.1
    CDS177..4811
    Translation

    Target ORF information:

    RefSeq Version XM_017008858.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95559
    Clone ID Related Accession (Same CDS sequence) NM_001351727.1 , NM_001351727.2 , XM_017008859.1
    Accession Version XM_017008859.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product rap guanine nucleotide exchange factor 2 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864348.1
    CDS995..5569
    Translation

    Target ORF information:

    RefSeq Version XM_017008859.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008859.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT
    ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT
    AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT
    TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT
    GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT
    GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT
    CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC
    TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG
    CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG
    GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG
    AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA
    GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA
    GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT
    GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG
    TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA
    GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG
    CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG
    CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC
    CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA
    GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39237
    Clone ID Related Accession (Same CDS sequence) XM_006714421.3 , NM_001351724.1 , NM_001351724.2
    Accession Version NM_001351724.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-22
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095064.3, AC105316.5 and AC074344.5. This sequence is a reference standard in the RefSeqGene project. On May 24, 2017 this sequence version replaced XM_006714421.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.175373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338653.1
    CDS38..5095
    Translation

    Target ORF information:

    RefSeq Version NM_001351724.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351724.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39237
    Clone ID Related Accession (Same CDS sequence) XM_006714421.3 , NM_001351724.1 , NM_001351724.2
    Accession Version NM_001351724.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095064.3, AC105316.5 and AC074344.5. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001351724.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.175373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338653.1
    CDS113..5170
    Translation

    Target ORF information:

    RefSeq Version NM_001351724.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351724.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63163
    Clone ID Related Accession (Same CDS sequence) NM_001351728.1 , NM_001351728.2 , XM_011532427.2
    Accession Version NM_001351728.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-08-03
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_011532427.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.113940.1, SRR1803611.140636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC
    AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338657.1
    CDS478..5100
    Translation

    Target ORF information:

    RefSeq Version NM_001351728.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC
    AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63163
    Clone ID Related Accession (Same CDS sequence) NM_001351728.1 , NM_001351728.2 , XM_011532427.2
    Accession Version NM_001351728.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On Jun 2, 2019 this sequence version replaced NM_001351728.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.140636.1, SRR1803615.113940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC
    AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338657.1
    CDS348..4970
    Translation

    Target ORF information:

    RefSeq Version NM_001351728.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351728.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC
    AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39234
    Clone ID Related Accession (Same CDS sequence) XM_005263358.3
    Accession Version XM_005263358.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GTGAGAACAG TAACAAGAAT
    AACAATGCAC CACGGACCTA TGGGATAGGC TATACTTTGG CTCCCAGTGG TACTGTGGAT
    AATTTTTCAG ATTCTGGTCA CAGTGAAATT TCTTCACGAT CCAGTATTGT TAGCAATTCG
    TCTTTTGACT CAGTGCCAGT CTCACTGCAC GATGAGAGGC GCCAGAGGCA TTCTGTCAGC
    ATCGTGGAAA CAAACCTAGG GATGGGCAGG ATGGAGAGGC GGACCATGAT TGAACCTGAT
    CAGTATAGCT TGGGGTCCTA TGCACCAATG TCCGAGGGCC GAGGCTTATA TGCTACAGCT
    ACAGTAATTT CTTCTCCAAG CACAGAGGAA CTTTCCCAGG ATCAGGGGGA TCGCGCGTCA
    CTTGATGCTG CTGACAGTGG CCGTGGGAGC TGGACGTCAT GCTCAAGTGG CTCCCATGAT
    AATATACAGA CGATCCAGCA CCAGAGAAGC TGGGAGACTC TTCCATTCGG GCATACTCAC
    TTTGATTATT CAGGGGATCC TGCAGGTTTA TGGGCATCAA GCAGCCATAT GGACCAAATT
    ATGTTTTCTG ATCATAGCAC AAAGTATAAC AGGCAAAATC AAAGTAGAGA GAGCCTTGAA
    CAAGCCCAGT CCCGAGCAAG CTGGGCGTCT TCCACAGGTT ACTGGGGAGA AGACTCAGAA
    GGTGACACAG GCACAATAAA GCGGAGGGGT GGAAAGGATG TTTCCATTGA AGCCGAAAGC
    AGTAGCCTAA CGTCTGTGAC TACGGAAGAA ACCAAGCCTG TCCCCATGCC TGCCCACATA
    GCTGTGGCAT CAAGTACTAC AAAGGGGCTC ATTGCACGAA AGGAGGGCAG GTATCGAGAG
    CCCCCGCCCA CCCCTCCCGG CTACATTGGA ATTCCCATTA CTGACTTTCC AGAAGGGCAC
    TCCCATCCAG CCAGGAAACC GCCGGACTAC AACGTGGCCC TTCAGAGATC GCGGATGGTC
    GCACGATCCT CCGACACAGC TGGGCCTTCA TCCGTACAGC AGCCACATGG GCATCCCACC
    AGCAGCAGGC CTGTGAACAA ACCTCAGTGG CATAAACCGA ACGAGTCTGA CCCGCGCCTC
    GCCCCCTATC AGTCCCAAGG GTTTTCCACC GAGGAGGATG AAGATGAACA AGTTTCTGCT
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263415.1
    CDS95..5200
    Translation

    Target ORF information:

    RefSeq Version XM_005263358.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263358.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GTGAGAACAG TAACAAGAAT
    AACAATGCAC CACGGACCTA TGGGATAGGC TATACTTTGG CTCCCAGTGG TACTGTGGAT
    AATTTTTCAG ATTCTGGTCA CAGTGAAATT TCTTCACGAT CCAGTATTGT TAGCAATTCG
    TCTTTTGACT CAGTGCCAGT CTCACTGCAC GATGAGAGGC GCCAGAGGCA TTCTGTCAGC
    ATCGTGGAAA CAAACCTAGG GATGGGCAGG ATGGAGAGGC GGACCATGAT TGAACCTGAT
    CAGTATAGCT TGGGGTCCTA TGCACCAATG TCCGAGGGCC GAGGCTTATA TGCTACAGCT
    ACAGTAATTT CTTCTCCAAG CACAGAGGAA CTTTCCCAGG ATCAGGGGGA TCGCGCGTCA
    CTTGATGCTG CTGACAGTGG CCGTGGGAGC TGGACGTCAT GCTCAAGTGG CTCCCATGAT
    AATATACAGA CGATCCAGCA CCAGAGAAGC TGGGAGACTC TTCCATTCGG GCATACTCAC
    TTTGATTATT CAGGGGATCC TGCAGGTTTA TGGGCATCAA GCAGCCATAT GGACCAAATT
    ATGTTTTCTG ATCATAGCAC AAAGTATAAC AGGCAAAATC AAAGTAGAGA GAGCCTTGAA
    CAAGCCCAGT CCCGAGCAAG CTGGGCGTCT TCCACAGGTT ACTGGGGAGA AGACTCAGAA
    GGTGACACAG GCACAATAAA GCGGAGGGGT GGAAAGGATG TTTCCATTGA AGCCGAAAGC
    AGTAGCCTAA CGTCTGTGAC TACGGAAGAA ACCAAGCCTG TCCCCATGCC TGCCCACATA
    GCTGTGGCAT CAAGTACTAC AAAGGGGCTC ATTGCACGAA AGGAGGGCAG GTATCGAGAG
    CCCCCGCCCA CCCCTCCCGG CTACATTGGA ATTCCCATTA CTGACTTTCC AGAAGGGCAC
    TCCCATCCAG CCAGGAAACC GCCGGACTAC AACGTGGCCC TTCAGAGATC GCGGATGGTC
    GCACGATCCT CCGACACAGC TGGGCCTTCA TCCGTACAGC AGCCACATGG GCATCCCACC
    AGCAGCAGGC CTGTGAACAA ACCTCAGTGG CATAAACCGA ACGAGTCTGA CCCGCGCCTC
    GCCCCCTATC AGTCCCAAGG GTTTTCCACC GAGGAGGATG AAGATGAACA AGTTTCTGCT
    GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39235
    Clone ID Related Accession (Same CDS sequence) XM_006714420.3
    Accession Version XM_006714420.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714420.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC
    ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT
    TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC
    ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC
    GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC
    ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA
    CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA
    GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT
    GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT
    CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG
    GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA
    AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA
    GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT
    CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA
    GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG
    ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT
    GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA
    GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG
    CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC
    AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC
    CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT
    GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC
    ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG
    CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA
    TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT
    AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG
    CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT
    AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC
    ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA
    AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG
    TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC
    TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA
    TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC
    CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC
    TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC
    TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC
    ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC
    TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT
    CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG
    AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT
    GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA
    AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG
    CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC
    ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT
    CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA
    CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG
    AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA
    AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT
    ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA
    AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA TCTTTTGGCT CCGGGCAGTT GGACTTAACC
    AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC AGTAACAAGA ATAACAATGC ACCACGGACC
    TATGGGATAG GCTATACTTT GGCTCCCAGT GGTACTGTGG ATAATTTTTC AGATTCTGGT
    CACAGTGAAA TTTCTTCACG ATCCAGTATT GTTAGCAATT CGTCTTTTGA CTCAGTGCCA
    GTCTCACTGC ACGATGAGAG GCGCCAGAGG CATTCTGTCA GCATCGTGGA AACAAACCTA
    GGGATGGGCA GGATGGAGAG GCGGACCATG ATTGAACCTG ATCAGTATAG CTTGGGGTCC
    TATGCACCAA TGTCCGAGGG CCGAGGCTTA TATGCTACAG CTACAGTAAT TTCTTCTCCA
    AGCACAGAGG AACTTTCCCA GGATCAGGGG GATCGCGCGT CACTTGATGC TGCTGACAGT
    GGCCGTGGGA GCTGGACGTC ATGCTCAAGT GGCTCCCATG ATAATATACA GACGATCCAG
    CACCAGAGAA GCTGGGAGAC TCTTCCATTC GGGCATACTC ACTTTGATTA TTCAGGGGAT
    CCTGCAGGTT TATGGGCATC AAGCAGCCAT ATGGACCAAA TTATGTTTTC TGATCATAGC
    ACAAAGTATA ACAGGCAAAA TCAAAGTAGA GAGAGCCTTG AACAAGCCCA GTCCCGAGCA
    AGCTGGGCGT CTTCCACAGG TTACTGGGGA GAAGACTCAG AAGGTGACAC AGGCACAATA
    AAGCGGAGGG GTGGAAAGGA TGTTTCCATT GAAGCCGAAA GCAGTAGCCT AACGTCTGTG
    ACTACGGAAG AAACCAAGCC TGTCCCCATG CCTGCCCACA TAGCTGTGGC ATCAAGTACT
    ACAAAGGGGC TCATTGCACG AAAGGAGGGC AGGTATCGAG AGCCCCCGCC CACCCCTCCC
    GGCTACATTG GAATTCCCAT TACTGACTTT CCAGAAGGGC ACTCCCATCC AGCCAGGAAA
    CCGCCGGACT ACAACGTGGC CCTTCAGAGA TCGCGGATGG TCGCACGATC CTCCGACACA
    GCTGGGCCTT CATCCGTACA GCAGCCACAT GGGCATCCCA CCAGCAGCAG GCCTGTGAAC
    AAACCTCAGT GGCATAAACC GAACGAGTCT GACCCGCGCC TCGCCCCCTA TCAGTCCCAA
    GGGTTTTCCA CCGAGGAGGA TGAAGATGAA CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714483.1
    CDS95..5182
    Translation

    Target ORF information:

    RefSeq Version XM_006714420.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714420.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC
    ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT
    TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC
    ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC
    GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC
    ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA
    CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA
    GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT
    GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT
    CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG
    GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA
    AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA
    GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT
    CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA
    GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG
    ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT
    GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA
    GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG
    CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC
    AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC
    CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT
    GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC
    ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG
    CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA
    TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT
    AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG
    CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT
    AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC
    ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA
    AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG
    TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC
    TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA
    TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC
    CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC
    TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC
    TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC
    ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC
    TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT
    CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG
    AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT
    GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA
    AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG
    CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC
    ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT
    CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA
    CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG
    AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA
    AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT
    ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA
    AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA TCTTTTGGCT CCGGGCAGTT GGACTTAACC
    AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC AGTAACAAGA ATAACAATGC ACCACGGACC
    TATGGGATAG GCTATACTTT GGCTCCCAGT GGTACTGTGG ATAATTTTTC AGATTCTGGT
    CACAGTGAAA TTTCTTCACG ATCCAGTATT GTTAGCAATT CGTCTTTTGA CTCAGTGCCA
    GTCTCACTGC ACGATGAGAG GCGCCAGAGG CATTCTGTCA GCATCGTGGA AACAAACCTA
    GGGATGGGCA GGATGGAGAG GCGGACCATG ATTGAACCTG ATCAGTATAG CTTGGGGTCC
    TATGCACCAA TGTCCGAGGG CCGAGGCTTA TATGCTACAG CTACAGTAAT TTCTTCTCCA
    AGCACAGAGG AACTTTCCCA GGATCAGGGG GATCGCGCGT CACTTGATGC TGCTGACAGT
    GGCCGTGGGA GCTGGACGTC ATGCTCAAGT GGCTCCCATG ATAATATACA GACGATCCAG
    CACCAGAGAA GCTGGGAGAC TCTTCCATTC GGGCATACTC ACTTTGATTA TTCAGGGGAT
    CCTGCAGGTT TATGGGCATC AAGCAGCCAT ATGGACCAAA TTATGTTTTC TGATCATAGC
    ACAAAGTATA ACAGGCAAAA TCAAAGTAGA GAGAGCCTTG AACAAGCCCA GTCCCGAGCA
    AGCTGGGCGT CTTCCACAGG TTACTGGGGA GAAGACTCAG AAGGTGACAC AGGCACAATA
    AAGCGGAGGG GTGGAAAGGA TGTTTCCATT GAAGCCGAAA GCAGTAGCCT AACGTCTGTG
    ACTACGGAAG AAACCAAGCC TGTCCCCATG CCTGCCCACA TAGCTGTGGC ATCAAGTACT
    ACAAAGGGGC TCATTGCACG AAAGGAGGGC AGGTATCGAG AGCCCCCGCC CACCCCTCCC
    GGCTACATTG GAATTCCCAT TACTGACTTT CCAGAAGGGC ACTCCCATCC AGCCAGGAAA
    CCGCCGGACT ACAACGTGGC CCTTCAGAGA TCGCGGATGG TCGCACGATC CTCCGACACA
    GCTGGGCCTT CATCCGTACA GCAGCCACAT GGGCATCCCA CCAGCAGCAG GCCTGTGAAC
    AAACCTCAGT GGCATAAACC GAACGAGTCT GACCCGCGCC TCGCCCCCTA TCAGTCCCAA
    GGGTTTTCCA CCGAGGAGGA TGAAGATGAA CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39236
    Clone ID Related Accession (Same CDS sequence) XM_005263359.3 , XM_005263359.4
    Accession Version XM_005263359.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC
    TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG
    ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT
    GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA
    CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG
    GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA
    CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA
    GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT
    GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG
    AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA
    GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG
    TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG
    GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG
    AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG
    GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG
    GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC
    ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG
    GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG
    CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT
    TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263416.1
    CDS97..5178
    Translation

    Target ORF information:

    RefSeq Version XM_005263359.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC
    TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG
    ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT
    GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA
    CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG
    GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA
    CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA
    GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT
    GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG
    AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA
    GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG
    TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG
    GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG
    AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG
    GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG
    GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC
    ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG
    GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG
    CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT
    TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39236
    Clone ID Related Accession (Same CDS sequence) XM_005263359.3 , XM_005263359.4
    Accession Version XM_005263359.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC
    TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG
    ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT
    GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA
    CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG
    GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA
    CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA
    GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT
    GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG
    AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA
    GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG
    TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG
    GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG
    AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG
    GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG
    GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC
    ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG
    GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG
    CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT
    TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263416.1
    CDS95..5176
    Translation

    Target ORF information:

    RefSeq Version XM_005263359.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263359.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT
    CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT
    TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA
    ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG
    CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG
    GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA
    CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA
    GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT
    CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA
    TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA
    GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC
    TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG
    ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT
    GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA
    CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG
    GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA
    CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA
    GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT
    GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG
    AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA
    GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG
    TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG
    GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG
    AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG
    GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG
    GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC
    ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG
    GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG
    CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT
    CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT
    TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39237
    Clone ID Related Accession (Same CDS sequence) XM_006714421.3 , NM_001351724.1 , NM_001351724.2
    Accession Version XM_006714421.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714484.1
    CDS62..5119
    Translation

    Target ORF information:

    RefSeq Version XM_006714421.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714421.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC
    GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39238
    Clone ID Related Accession (Same CDS sequence) XM_005263360.3
    Accession Version XM_005263360.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263417.1
    CDS89..5071
    Translation

    Target ORF information:

    RefSeq Version XM_005263360.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263360.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT
    CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC
    CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT
    TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA
    GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT
    CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA
    GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG
    ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA
    GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA
    TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT
    GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT
    GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA
    AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA
    CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA
    ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG
    TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG
    TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT
    CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC
    AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT
    AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT
    ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT
    AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA
    AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA
    TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA
    GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC
    TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC
    AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG
    ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA
    GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT
    GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC
    AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC
    CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG
    GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT
    GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA
    CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT
    GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG
    GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT
    GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA
    TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA
    AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG
    GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG
    GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT
    CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT
    CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC
    CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG
    ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC
    ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA
    AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT
    TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG
    TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG
    TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC
    GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG
    CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG
    TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC
    TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC
    AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT
    TCTCCACAGA GTTCTCCAAG GAAAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39239
    Clone ID Related Accession (Same CDS sequence) XM_005263361.3
    Accession Version XM_005263361.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC
    ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT
    TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC
    ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC
    GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC
    ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA
    CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA
    GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT
    GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT
    CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG
    GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA
    AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA
    GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT
    CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA
    GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG
    ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT
    GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA
    GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG
    CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC
    AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC
    CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT
    GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC
    ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG
    CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA
    TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT
    AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG
    CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT
    AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC
    ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA
    AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG
    TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC
    TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA
    TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC
    CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC
    TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC
    TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC
    ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC
    TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT
    CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG
    AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT
    GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA
    AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG
    CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC
    ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT
    CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA
    CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG
    AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA
    AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT
    ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA
    AGGAAAGGCT ATACTTTGGC TCCCAGTGGT ACTGTGGATA ATTTTTCAGA TTCTGGTCAC
    AGTGAAATTT CTTCACGATC CAGTATTGTT AGCAATTCGT CTTTTGACTC AGTGCCAGTC
    TCACTGCACG ATGAGAGGCG CCAGAGGCAT TCTGTCAGCA TCGTGGAAAC AAACCTAGGG
    ATGGGCAGGA TGGAGAGGCG GACCATGATT GAACCTGATC AGTATAGCTT GGGGTCCTAT
    GCACCAATGT CCGAGGGCCG AGGCTTATAT GCTACAGCTA CAGTAATTTC TTCTCCAAGC
    ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT CGCGCGTCAC TTGATGCTGC TGACAGTGGC
    CGTGGGAGCT GGACGTCATG CTCAAGTGGC TCCCATGATA ATATACAGAC GATCCAGCAC
    CAGAGAAGCT GGGAGACTCT TCCATTCGGG CATACTCACT TTGATTATTC AGGGGATCCT
    GCAGGTTTAT GGGCATCAAG CAGCCATATG GACCAAATTA TGTTTTCTGA TCATAGCACA
    AAGTATAACA GGCAAAATCA AAGTAGAGAG AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC
    TGGGCGTCTT CCACAGGTTA CTGGGGAGAA GACTCAGAAG GTGACACAGG CACAATAAAG
    CGGAGGGGTG GAAAGGATGT TTCCATTGAA GCCGAAAGCA GTAGCCTAAC GTCTGTGACT
    ACGGAAGAAA CCAAGCCTGT CCCCATGCCT GCCCACATAG CTGTGGCATC AAGTACTACA
    AAGGGGCTCA TTGCACGAAA GGAGGGCAGG TATCGAGAGC CCCCGCCCAC CCCTCCCGGC
    TACATTGGAA TTCCCATTAC TGACTTTCCA GAAGGGCACT CCCATCCAGC CAGGAAACCG
    CCGGACTACA ACGTGGCCCT TCAGAGATCG CGGATGGTCG CACGATCCTC CGACACAGCT
    GGGCCTTCAT CCGTACAGCA GCCACATGGG CATCCCACCA GCAGCAGGCC TGTGAACAAA
    CCTCAGTGGC ATAAACCGAA CGAGTCTGAC CCGCGCCTCG CCCCCTATCA GTCCCAAGGG
    TTTTCCACCG AGGAGGATGA AGATGAACAA GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263418.1
    CDS95..5059
    Translation

    Target ORF information:

    RefSeq Version XM_005263361.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263361.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
    ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC
    TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA
    AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT
    TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA
    GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG
    GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA
    AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT
    CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC
    ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT
    TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC
    ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC
    GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC
    ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA
    CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA
    GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT
    GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT
    CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG
    GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC
    ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT
    GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA
    AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA
    GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT
    CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC
    AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA
    GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA
    ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG
    ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT
    GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA
    GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG
    CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC
    AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC
    CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT
    GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC
    ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG
    CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA
    TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT
    AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG
    CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT
    AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC
    ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA
    AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT
    TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG
    TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC
    TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA
    TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC
    CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG
    AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC
    TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC
    TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT
    CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC
    ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC
    TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT
    CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG
    AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT
    GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA
    AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG
    CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC
    ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT
    CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA
    CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG
    AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA
    AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT
    ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA
    AGGAAAGGCT ATACTTTGGC TCCCAGTGGT ACTGTGGATA ATTTTTCAGA TTCTGGTCAC
    AGTGAAATTT CTTCACGATC CAGTATTGTT AGCAATTCGT CTTTTGACTC AGTGCCAGTC
    TCACTGCACG ATGAGAGGCG CCAGAGGCAT TCTGTCAGCA TCGTGGAAAC AAACCTAGGG
    ATGGGCAGGA TGGAGAGGCG GACCATGATT GAACCTGATC AGTATAGCTT GGGGTCCTAT
    GCACCAATGT CCGAGGGCCG AGGCTTATAT GCTACAGCTA CAGTAATTTC TTCTCCAAGC
    ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT CGCGCGTCAC TTGATGCTGC TGACAGTGGC
    CGTGGGAGCT GGACGTCATG CTCAAGTGGC TCCCATGATA ATATACAGAC GATCCAGCAC
    CAGAGAAGCT GGGAGACTCT TCCATTCGGG CATACTCACT TTGATTATTC AGGGGATCCT
    GCAGGTTTAT GGGCATCAAG CAGCCATATG GACCAAATTA TGTTTTCTGA TCATAGCACA
    AAGTATAACA GGCAAAATCA AAGTAGAGAG AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC
    TGGGCGTCTT CCACAGGTTA CTGGGGAGAA GACTCAGAAG GTGACACAGG CACAATAAAG
    CGGAGGGGTG GAAAGGATGT TTCCATTGAA GCCGAAAGCA GTAGCCTAAC GTCTGTGACT
    ACGGAAGAAA CCAAGCCTGT CCCCATGCCT GCCCACATAG CTGTGGCATC AAGTACTACA
    AAGGGGCTCA TTGCACGAAA GGAGGGCAGG TATCGAGAGC CCCCGCCCAC CCCTCCCGGC
    TACATTGGAA TTCCCATTAC TGACTTTCCA GAAGGGCACT CCCATCCAGC CAGGAAACCG
    CCGGACTACA ACGTGGCCCT TCAGAGATCG CGGATGGTCG CACGATCCTC CGACACAGCT
    GGGCCTTCAT CCGTACAGCA GCCACATGGG CATCCCACCA GCAGCAGGCC TGTGAACAAA
    CCTCAGTGGC ATAAACCGAA CGAGTCTGAC CCGCGCCTCG CCCCCTATCA GTCCCAAGGG
    TTTTCCACCG AGGAGGATGA AGATGAACAA GTTTCTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39240
    Clone ID Related Accession (Same CDS sequence) XM_006714422.3 , XM_006714422.4 , XM_011532426.2
    Accession Version XM_006714422.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA
    TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC
    AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714485.1
    CDS658..5415
    Translation

    Target ORF information:

    RefSeq Version XM_006714422.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714422.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA
    TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC
    AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39240
    Clone ID Related Accession (Same CDS sequence) XM_006714422.3 , XM_006714422.4 , XM_011532426.2
    Accession Version XM_006714422.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714422.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA
    TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC
    AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714485.1
    CDS2169..6926
    Translation

    Target ORF information:

    RefSeq Version XM_006714422.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714422.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA
    TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC
    AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39240
    Clone ID Related Accession (Same CDS sequence) XM_006714422.3 , XM_006714422.4 , XM_011532426.2
    Accession Version XM_011532426.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA
    TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC
    AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530728.1
    CDS1210..5967
    Translation

    Target ORF information:

    RefSeq Version XM_011532426.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532426.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
    AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT
    GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA
    TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC
    CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG
    AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG
    AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG
    AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA
    ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT
    TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG
    GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG
    ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG
    GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA
    ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC
    AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA
    GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC
    TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA
    GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG
    TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG
    TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA
    GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC
    GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT
    CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT
    GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA
    GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT
    AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA
    AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA
    AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT
    GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG
    ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT
    GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA
    TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC
    ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT
    GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT
    CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC
    TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT
    GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT
    CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG
    CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC
    ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC
    AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT
    CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA
    CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC
    CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA
    CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT
    GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG
    GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG
    AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG
    GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC
    CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG
    GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA
    AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA
    TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG
    CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG
    TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG
    GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT
    TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT
    TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT
    TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA
    TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC
    AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT
    GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT
    GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG
    CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG
    ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA
    TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG
    GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT
    GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC
    GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT
    ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA
    GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA
    GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT
    GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG
    CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC
    AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT
    CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA
    TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT
    GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT
    GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA
    CAAGTTTCTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63162
    Clone ID Related Accession (Same CDS sequence) XM_011532425.2
    Accession Version XM_011532425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rap guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGAAT ATGGGTTATC TGTGGACTAT ATGGATGAAA ATGAAGAATA TTTTCAGCGG 
    CAAGCTTCCC ATAGACAGTC TCGAAGGAGA TTTAGAAAAA TCAACCAGAA AGGTGAAAGA
    CAAACAATTA TTGACACTGT GGATCCTTAT CCCATGGGCA AACCTCCTTT GCCTAGAGGC
    TATCACACGG AATGCACTAA ATCTCAGCTT CCTGCAGATT TCACAAAACT GCATCTTACT
    GACAGTCTCC ACCCACAGGT GACCCACGTT TCTTCTAGCC ATTCAGGATG TAGTATCACT
    AGTGATTCTG GGAGCAGCAG TCTTTCTGAT ATCTACCAGG CCACAGAAAG CGAGGCTGGT
    GATATGGACC TGAGTGGGTT GCCAGAAACA GCAGTGGATT CCGAAGACGA CGACGATGAA
    GAAGACATTG AGAGAGCATC AGATCCTCTG ATGAGCAGGG ACATTGTGAG AGACTGCCTA
    GAGAAGGACC CAATTGACCG GACAGATGAT GACATTGAAC AACTCTTGGA ATTTATGCAC
    CAGTTGCCTG CTTTTGCCAA TATGACAATG TCAGTGAGGC GAGAACTCTG TGCTGTGATG
    GTGTTCGCAG TGGTGGAAAG AGCAGGGACC ATAGTGTTAA ATGATGGTGA AGAGCTGGAC
    TCCTGGTCAG TGATTCTCAA TGGATCTGTG GAAGTGACTT ATCCAGATGG AAAAGCAGAA
    ATACTGTGCA TGGGAAATAG TTTTGGTGTC TCTCCTACCA TGGACAAAGA ATACATGAAA
    GGAGTGATGA GAACAAAGGT GGATGACTGC CAGTTTGTCT GCATAGCCCA GCAAGATTAC
    TGCCGTATTC TCAATCAAGT AGAAAAGAAC ATGCAAAAAG TTGAAGAGGA AGGAGAGATT
    GTTATGGTGA AAGAACACCG AGAACTTGAT CGAACTGGAA CAAGAAAGGG ACACATTGTC
    ATCAAGGGTA CCTCAGAAAG GTTAACAATG CATTTGGTGG AAGAGCATTC AGTAGTAGAT
    CCAACATTCA TAGAAGACTT TCTGTTGACC TATAGGACTT TTCTTTCTAG CCCAATGGAA
    GTGGGCAAAA AGTTATTGGA GTGGTTTAAT GACCCGAGCC TCAGGGATAA GGTTACACGG
    GTAGTATTAT TGTGGGTAAA TAATCACTTC AATGACTTTG AAGGAGATCC TGCAATGACT
    CGATTTTTAG AAGAATTTGA AAACAATCTG GAAAGAGAGA AAATGGGTGG ACACCTAAGG
    CTGTTGAATA TCGCGTGTGC TGCTAAAGCA AAAAGAAGAT TGATGACGTT AACAAAACCA
    TCCCGAGAAG CTCCTTTGCC TTTTATCTTA CTTGGAGGCT CTGAGAAGGG ATTTGGAATC
    TTTGTTGACA GTGTAGATTC AGGTAGCAAA GCAACTGAAG CAGGCTTGAA ACGGGGGGAT
    CAGATATTAG AAGTAAATGG CCAAAACTTT GAAAACATTC AGCTGTCAAA AGCTATGGAA
    ATTCTTAGAA ATAACACACA TTTATCTATC ACTGTGAAAA CCAATTTATT TGTATTTAAA
    GAACTTCTAA CAAGATTGTC AGAAGAGAAA AGAAATGGTG CCCCCCACCT TCCTAAAATT
    GGTGACATTA AAAAGGCCAG TCGCTACTCC ATTCCAGATC TTGCTGTAGA TGTAGAACAG
    GTGATAGGAC TTGAAAAAGT GAACAAAAAA AGTAAAGCCA ACACTGTGGG AGGAAGGAAC
    AAGCTGAAAA AGATACTCGA CAAGACTCGG ATCAGTATCT TGCCACAGAA ACCATACAAT
    GATATTGGGA TTGGTCAGTC TCAAGATGAC AGCATAGTAG GATTAAGGCA GACAAAGCAC
    ATCCCAACTG CATTGCCTGT CAGTGGAACC TTATCATCCA GTAATCCTGA TTTATTGCAG
    TCACATCATC GCATTTTAGA CTTCAGTGCT ACTCCTGACT TGCCAGATCA AGTGCTAAGG
    GTTTTTAAGG CTGATCAGCA AAGCCGCTAC ATCATGATCA GTAAGGACAC TACAGCAAAG
    GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT GCTGTTACTG CCACCCCGGA TCAATATTCA
    CTATGTGAGG TCTCTGTCAC ACCTGAGGGA GTAATCAAAC AAAGAAGACT TCCAGATCAG
    CTTTCCAAAC TTGCAGACAG AATACAACTG AGTGGAAGGT ATTATCTGAA AAACAACATG
    GAAACAGAAA CTCTTTGTTC AGATGAAGAT GCTCAGGAGT TGTTGAGAGA GAGTCAAATT
    TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT GCAACACAGC TCTCTATGCG AAATTTTGAA
    CTCTTTCGCA ACATTGAACC TACTGAATAT ATAGATGATT TATTTAAACT CAGATCAAAA
    ACCAGCTGTG CCAACCTGAA GAGATTTGAA GAAGTCATTA ACCAGGAAAC ATTTTGGGTA
    GCATCTGAAA TTCTCAGAGA AACAAACCAG CTGAAGAGGA TGAAGATCAT TAAGCATTTC
    ATCAAGATAG CACTGCACTG TAGGGAATGC AAGAATTTTA ACTCAATGTT TGCAATCATC
    AGTGGCCTAA ACCTGGCACC AGTGGCAAGA CTGCGAACGA CCTGGGAGAA ACTTCCCAAT
    AAATACGAAA AACTATTTCA AGATCTCCAA GACCTGTTTG ATCCTTCCAG AAACATGGCA
    AAATATCGTA ATGTTCTCAA TAGTCAAAAT CTACAACCTC CCATAATCCC TCTATTCCCA
    GTTATCAAAA AGGATCTCAC CTTCCTTCAC GAAGGAAATG ACTCAAAAGT AGACGGGCTG
    GTCAATTTTG AGAAGCTAAG GATGATTGCA AAAGAAATTC GTCACGTTGG CCGAATGGCT
    TCAGTGAACA TGGACCCTGC CCTCATGTTC AGGACTCGGA AGAAGAAATG GCGGAGTTTG
    GGGTCTCTCA GCCAGGGTAG TACAAATGCA ACAGTGCTAG ATGTTGCTCA GACAGGTGGT
    CATAAAAAGC GGGTACGTCG TAGTTCCTTT CTCAATGCCA AAAAGCTTTA TGAAGATGCC
    CAAATGGCTC GAAAAGTGAA GCAGTACCTT TCCAATTTGG AGCTAGAAAT GGACGAGGAG
    AGTCTTCAGA CATTATCTCT GCAGTGTGAG CCAGCAACCA ACACATTGCC TAAGAATCCT
    GGTGACAAAA AGCCTGTCAA ATCCGAGACC TCTCCAGTAG CTCCAAGGGC AGGGTCACAA
    CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG CAGCAGCCAC CACCAGCACA TAAAATCAAC
    CAGGGACTAC AGGTTCCCGC CGTGTCCCTT TATCCTTCAC GGAAGAAAGT GCCCGTAAAG
    GATCTCCCAC CTTTTGGCAT AAACTCTCCA CAAGCTTTAA AAAAAATTCT TTCTTTGTCT
    GAAGAAGGAA GTTTGGAACG TCACAAGAAA CAGGCTGAAG ATACAATATC AAATGCATCT
    TCGCAGCTTT CTTCTCCTCC TACTTCTCCA CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC
    AATCAGCTGA GATCTTTTGG CTCCGGGCAG TTGGACTTAA CCAGTTCCTC CTCTTCTCTT
    GGAAGTGAGA ACAGTAACAA GAATAACAAT GCACCACGGA CCTATGGGAT AGGCTATACT
    TTGGCTCCCA GTGGTACTGT GGATAATTTT TCAGATTCTG GTCACAGTGA AATTTCTTCA
    CGATCCAGTA TTGTTAGCAA TTCGTCTTTT GACTCAGTGC CAGTCTCACT GCACGATGAG
    AGGCGCCAGA GGCATTCTGT CAGCATCGTG GAAACAAACC TAGGGATGGG CAGGATGGAG
    AGGCGGACCA TGATTGAACC TGATCAGTAT AGCTTGGGGT CCTATGCACC AATGTCCGAG
    GGCCGAGGCT TATATGCTAC AGCTACAGTA ATTTCTTCTC CAAGCACAGA GGAACTTTCC
    CAGGATCAGG GGGATCGCGC GTCACTTGAT GCTGCTGACA GTGGCCGTGG GAGCTGGACG
    TCATGCTCAA GTGGCTCCCA TGATAATATA CAGACGATCC AGCACCAGAG AAGCTGGGAG
    ACTCTTCCAT TCGGGCATAC TCACTTTGAT TATTCAGGGG ATCCTGCAGG TTTATGGGCA
    TCAAGCAGCC ATATGGACCA AATTATGTTT TCTGATCATA GCACAAAGTA TAACAGGCAA
    AATCAAAGTA GAGAGAGCCT TGAACAAGCC CAGTCCCGAG CAAGCTGGGC GTCTTCCACA
    GGTTACTGGG GAGAAGACTC AGAAGGTGAC ACAGGCACAA TAAAGCGGAG GGGTGGAAAG
    GATGTTTCCA TTGAAGCCGA AAGCAGTAGC CTAACGTCTG TGACTACGGA AGAAACCAAG
    CCTGTCCCCA TGCCTGCCCA CATAGCTGTG GCATCAAGTA CTACAAAGGG GCTCATTGCA
    CGAAAGGAGG GCAGGTATCG AGAGCCCCCG CCCACCCCTC CCGGCTACAT TGGAATTCCC
    ATTACTGACT TTCCAGAAGG GCACTCCCAT CCAGCCAGGA AACCGCCGGA CTACAACGTG
    GCCCTTCAGA GATCGCGGAT GGTCGCACGA TCCTCCGACA CAGCTGGGCC TTCATCCGTA
    CAGCAGCCAC ATGGGCATCC CACCAGCAGC AGGCCTGTGA ACAAACCTCA GTGGCATAAA
    CCGAACGAGT CTGACCCGCG CCTCGCCCCC TATCAGTCCC AAGGGTTTTC CACCGAGGAG
    GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530727.1
    CDS261..5030
    Translation

    Target ORF information:

    RefSeq Version XM_011532425.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTGAAT ATGGGTTATC TGTGGACTAT ATGGATGAAA ATGAAGAATA TTTTCAGCGG 
    CAAGCTTCCC ATAGACAGTC TCGAAGGAGA TTTAGAAAAA TCAACCAGAA AGGTGAAAGA
    CAAACAATTA TTGACACTGT GGATCCTTAT CCCATGGGCA AACCTCCTTT GCCTAGAGGC
    TATCACACGG AATGCACTAA ATCTCAGCTT CCTGCAGATT TCACAAAACT GCATCTTACT
    GACAGTCTCC ACCCACAGGT GACCCACGTT TCTTCTAGCC ATTCAGGATG TAGTATCACT
    AGTGATTCTG GGAGCAGCAG TCTTTCTGAT ATCTACCAGG CCACAGAAAG CGAGGCTGGT
    GATATGGACC TGAGTGGGTT GCCAGAAACA GCAGTGGATT CCGAAGACGA CGACGATGAA
    GAAGACATTG AGAGAGCATC AGATCCTCTG ATGAGCAGGG ACATTGTGAG AGACTGCCTA
    GAGAAGGACC CAATTGACCG GACAGATGAT GACATTGAAC AACTCTTGGA ATTTATGCAC
    CAGTTGCCTG CTTTTGCCAA TATGACAATG TCAGTGAGGC GAGAACTCTG TGCTGTGATG
    GTGTTCGCAG TGGTGGAAAG AGCAGGGACC ATAGTGTTAA ATGATGGTGA AGAGCTGGAC
    TCCTGGTCAG TGATTCTCAA TGGATCTGTG GAAGTGACTT ATCCAGATGG AAAAGCAGAA
    ATACTGTGCA TGGGAAATAG TTTTGGTGTC TCTCCTACCA TGGACAAAGA ATACATGAAA
    GGAGTGATGA GAACAAAGGT GGATGACTGC CAGTTTGTCT GCATAGCCCA GCAAGATTAC
    TGCCGTATTC TCAATCAAGT AGAAAAGAAC ATGCAAAAAG TTGAAGAGGA AGGAGAGATT
    GTTATGGTGA AAGAACACCG AGAACTTGAT CGAACTGGAA CAAGAAAGGG ACACATTGTC
    ATCAAGGGTA CCTCAGAAAG GTTAACAATG CATTTGGTGG AAGAGCATTC AGTAGTAGAT
    CCAACATTCA TAGAAGACTT TCTGTTGACC TATAGGACTT TTCTTTCTAG CCCAATGGAA
    GTGGGCAAAA AGTTATTGGA GTGGTTTAAT GACCCGAGCC TCAGGGATAA GGTTACACGG
    GTAGTATTAT TGTGGGTAAA TAATCACTTC AATGACTTTG AAGGAGATCC TGCAATGACT
    CGATTTTTAG AAGAATTTGA AAACAATCTG GAAAGAGAGA AAATGGGTGG ACACCTAAGG
    CTGTTGAATA TCGCGTGTGC TGCTAAAGCA AAAAGAAGAT TGATGACGTT AACAAAACCA
    TCCCGAGAAG CTCCTTTGCC TTTTATCTTA CTTGGAGGCT CTGAGAAGGG ATTTGGAATC
    TTTGTTGACA GTGTAGATTC AGGTAGCAAA GCAACTGAAG CAGGCTTGAA ACGGGGGGAT
    CAGATATTAG AAGTAAATGG CCAAAACTTT GAAAACATTC AGCTGTCAAA AGCTATGGAA
    ATTCTTAGAA ATAACACACA TTTATCTATC ACTGTGAAAA CCAATTTATT TGTATTTAAA
    GAACTTCTAA CAAGATTGTC AGAAGAGAAA AGAAATGGTG CCCCCCACCT TCCTAAAATT
    GGTGACATTA AAAAGGCCAG TCGCTACTCC ATTCCAGATC TTGCTGTAGA TGTAGAACAG
    GTGATAGGAC TTGAAAAAGT GAACAAAAAA AGTAAAGCCA ACACTGTGGG AGGAAGGAAC
    AAGCTGAAAA AGATACTCGA CAAGACTCGG ATCAGTATCT TGCCACAGAA ACCATACAAT
    GATATTGGGA TTGGTCAGTC TCAAGATGAC AGCATAGTAG GATTAAGGCA GACAAAGCAC
    ATCCCAACTG CATTGCCTGT CAGTGGAACC TTATCATCCA GTAATCCTGA TTTATTGCAG
    TCACATCATC GCATTTTAGA CTTCAGTGCT ACTCCTGACT TGCCAGATCA AGTGCTAAGG
    GTTTTTAAGG CTGATCAGCA AAGCCGCTAC ATCATGATCA GTAAGGACAC TACAGCAAAG
    GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT GCTGTTACTG CCACCCCGGA TCAATATTCA
    CTATGTGAGG TCTCTGTCAC ACCTGAGGGA GTAATCAAAC AAAGAAGACT TCCAGATCAG
    CTTTCCAAAC TTGCAGACAG AATACAACTG AGTGGAAGGT ATTATCTGAA AAACAACATG
    GAAACAGAAA CTCTTTGTTC AGATGAAGAT GCTCAGGAGT TGTTGAGAGA GAGTCAAATT
    TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT GCAACACAGC TCTCTATGCG AAATTTTGAA
    CTCTTTCGCA ACATTGAACC TACTGAATAT ATAGATGATT TATTTAAACT CAGATCAAAA
    ACCAGCTGTG CCAACCTGAA GAGATTTGAA GAAGTCATTA ACCAGGAAAC ATTTTGGGTA
    GCATCTGAAA TTCTCAGAGA AACAAACCAG CTGAAGAGGA TGAAGATCAT TAAGCATTTC
    ATCAAGATAG CACTGCACTG TAGGGAATGC AAGAATTTTA ACTCAATGTT TGCAATCATC
    AGTGGCCTAA ACCTGGCACC AGTGGCAAGA CTGCGAACGA CCTGGGAGAA ACTTCCCAAT
    AAATACGAAA AACTATTTCA AGATCTCCAA GACCTGTTTG ATCCTTCCAG AAACATGGCA
    AAATATCGTA ATGTTCTCAA TAGTCAAAAT CTACAACCTC CCATAATCCC TCTATTCCCA
    GTTATCAAAA AGGATCTCAC CTTCCTTCAC GAAGGAAATG ACTCAAAAGT AGACGGGCTG
    GTCAATTTTG AGAAGCTAAG GATGATTGCA AAAGAAATTC GTCACGTTGG CCGAATGGCT
    TCAGTGAACA TGGACCCTGC CCTCATGTTC AGGACTCGGA AGAAGAAATG GCGGAGTTTG
    GGGTCTCTCA GCCAGGGTAG TACAAATGCA ACAGTGCTAG ATGTTGCTCA GACAGGTGGT
    CATAAAAAGC GGGTACGTCG TAGTTCCTTT CTCAATGCCA AAAAGCTTTA TGAAGATGCC
    CAAATGGCTC GAAAAGTGAA GCAGTACCTT TCCAATTTGG AGCTAGAAAT GGACGAGGAG
    AGTCTTCAGA CATTATCTCT GCAGTGTGAG CCAGCAACCA ACACATTGCC TAAGAATCCT
    GGTGACAAAA AGCCTGTCAA ATCCGAGACC TCTCCAGTAG CTCCAAGGGC AGGGTCACAA
    CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG CAGCAGCCAC CACCAGCACA TAAAATCAAC
    CAGGGACTAC AGGTTCCCGC CGTGTCCCTT TATCCTTCAC GGAAGAAAGT GCCCGTAAAG
    GATCTCCCAC CTTTTGGCAT AAACTCTCCA CAAGCTTTAA AAAAAATTCT TTCTTTGTCT
    GAAGAAGGAA GTTTGGAACG TCACAAGAAA CAGGCTGAAG ATACAATATC AAATGCATCT
    TCGCAGCTTT CTTCTCCTCC TACTTCTCCA CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC
    AATCAGCTGA GATCTTTTGG CTCCGGGCAG TTGGACTTAA CCAGTTCCTC CTCTTCTCTT
    GGAAGTGAGA ACAGTAACAA GAATAACAAT GCACCACGGA CCTATGGGAT AGGCTATACT
    TTGGCTCCCA GTGGTACTGT GGATAATTTT TCAGATTCTG GTCACAGTGA AATTTCTTCA
    CGATCCAGTA TTGTTAGCAA TTCGTCTTTT GACTCAGTGC CAGTCTCACT GCACGATGAG
    AGGCGCCAGA GGCATTCTGT CAGCATCGTG GAAACAAACC TAGGGATGGG CAGGATGGAG
    AGGCGGACCA TGATTGAACC TGATCAGTAT AGCTTGGGGT CCTATGCACC AATGTCCGAG
    GGCCGAGGCT TATATGCTAC AGCTACAGTA ATTTCTTCTC CAAGCACAGA GGAACTTTCC
    CAGGATCAGG GGGATCGCGC GTCACTTGAT GCTGCTGACA GTGGCCGTGG GAGCTGGACG
    TCATGCTCAA GTGGCTCCCA TGATAATATA CAGACGATCC AGCACCAGAG AAGCTGGGAG
    ACTCTTCCAT TCGGGCATAC TCACTTTGAT TATTCAGGGG ATCCTGCAGG TTTATGGGCA
    TCAAGCAGCC ATATGGACCA AATTATGTTT TCTGATCATA GCACAAAGTA TAACAGGCAA
    AATCAAAGTA GAGAGAGCCT TGAACAAGCC CAGTCCCGAG CAAGCTGGGC GTCTTCCACA
    GGTTACTGGG GAGAAGACTC AGAAGGTGAC ACAGGCACAA TAAAGCGGAG GGGTGGAAAG
    GATGTTTCCA TTGAAGCCGA AAGCAGTAGC CTAACGTCTG TGACTACGGA AGAAACCAAG
    CCTGTCCCCA TGCCTGCCCA CATAGCTGTG GCATCAAGTA CTACAAAGGG GCTCATTGCA
    CGAAAGGAGG GCAGGTATCG AGAGCCCCCG CCCACCCCTC CCGGCTACAT TGGAATTCCC
    ATTACTGACT TTCCAGAAGG GCACTCCCAT CCAGCCAGGA AACCGCCGGA CTACAACGTG
    GCCCTTCAGA GATCGCGGAT GGTCGCACGA TCCTCCGACA CAGCTGGGCC TTCATCCGTA
    CAGCAGCCAC ATGGGCATCC CACCAGCAGC AGGCCTGTGA ACAAACCTCA GTGGCATAAA
    CCGAACGAGT CTGACCCGCG CCTCGCCCCC TATCAGTCCC AAGGGTTTTC CACCGAGGAG
    GATGAAGATG AACAAGTTTC TGCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63163
    Clone ID Related Accession (Same CDS sequence) NM_001351728.1 , NM_001351728.2 , XM_011532427.2
    Accession Version XM_011532427.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product rap guanine nucleotide exchange factor 2 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC
    AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011530729.1
    CDS995..5617
    Translation

    Target ORF information:

    RefSeq Version XM_011532427.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011532427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
    CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC
    GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT
    GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA
    ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT
    CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT
    GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA
    ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG
    ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT
    GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT
    GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC
    TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG
    AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT
    GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA
    ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG
    ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT
    AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC
    TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT
    CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA
    GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC
    TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC
    AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC
    TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT
    ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG
    AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC
    TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA
    AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT
    CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT
    GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA
    ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT
    GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC
    TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG
    TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG
    GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA
    CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA
    GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA
    GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA
    TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT
    GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC
    CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA
    TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA
    AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC
    CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA
    AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT
    CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT
    GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG
    TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT
    GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC
    TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC
    CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT
    GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG
    ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC
    CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC
    CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT
    CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG
    AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT
    CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG
    CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC
    AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT
    TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT
    TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC
    GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG
    TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA
    GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT
    GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT
    ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT
    GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG
    TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA
    GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT
    GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT
    AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT
    GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC
    CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC
    CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA
    CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC
    AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC
    CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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