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MAGI2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAGI2
Entrez Gene ID 9863
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 2
Synonyms ACVRIP1,AIP-1,AIP1,ARIP1,MAGI-2,SSCAM
General protein information
Preferred Names

membrane associated guanylate kinase, WW and PDZ domain containing 2

Names

membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
activin receptor interacting protein 1
atrophin-1-interacting protein 1
atrophin-1-interacting protein A
membrane-associated guanylate kinase inverted 2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q21.11

Summary The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq, Jul 2008].
Disorder MIM:

606382


mRNA and Protein(s)

mRNA Protein Name
XM_017012840.1 XP_016868329.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X1
XM_017012842.1 XP_016868331.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X3
XM_011516718.1 XP_011515020.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X6
XM_017012848.1 XP_016868337.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X10
NM_001301128.1 NP_001288057.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform 2
XM_017012843.1 XP_016868332.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4
XM_017012847.1 XP_016868336.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
XM_017012849.1 XP_016868338.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X11
XM_011516726.2 XP_011515028.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X15
XM_011516728.1 XP_011515030.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X16
XM_011516720.2 XP_011515022.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
XM_017012850.1 XP_016868339.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X12
XM_017012851.1 XP_016868340.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X13
XM_017012845.1 XP_016868334.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X7
XM_011516719.2 XP_011515021.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
XM_017012841.1 XP_016868330.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X2
XM_017012844.1 XP_016868333.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X5
XM_017012846.1 XP_016868335.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X8
XM_017012852.1 XP_016868341.1 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X14
NM_012301.3 NP_036433.2 membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform 1



Gallus gallus (chicken) MAGI2 NP_001186469.1
Danio rerio (zebrafish) magi2 NP_001116083.1
Homo sapiens (human) MAGI2 NP_036433.2
Mus musculus (house mouse) Magi2 NP_001164217.1
Caenorhabditis elegans (roundworm) magi-1 NP_001255577.1
Pan troglodytes (chimpanzee) MAGI2 XP_519172.3
Rattus norvegicus (Norway rat) Magi2 NP_446073.1
Canis lupus familiaris (dog) MAGI2 XP_005631075.1
Bos taurus (cattle) MAGI2 XP_002686815.1


Related articles in PubMed

Novel genes for airway wall thickness identified with combined genome-wide association and expression analyses.
Dijkstra AE, Postma DS, van Ginneken B, Wielpütz MO, Schmidt M, Becker N, Owsijewitsch M, Kauczor HU, de Koning HJ, Lammers JW, Oudkerk M, Brandsma CA, Bossé Y, Nickle DC, Sin DD, Hiemstra PS, Wijmenga C, Smolonska J, Zanen P, Vonk JM, van den Berge M, Boezen HM, Groen HJ
American journal of respiratory and critical care medicine191(5)547-56(2015 Mar)

Genome-wide association study identifies multiple loci associated with both mammographic density and breast cancer risk.
Lindström S, Thompson DJ, Paterson AD, Li J, Gierach GL, Scott C, Stone J, Douglas JA, dos-Santos-Silva I, Fernandez-Navarro P, Verghase J, Smith P, Brown J, Luben R, Wareham NJ, Loos RJ, Heit JA, Pankratz VS, Norman A, Goode EL, Cunningham JM, deAndrade M, Vierkant RA, Czene K, Fasching PA, Baglietto L, Southey MC, Giles GG, Shah KP, Chan HP, Helvie MA, Beck AH, Knoblauch NW, Hazra A, Hunter DJ, Kraft P, Pollan M, Figueroa JD, Couch FJ, Hopper JL, Hall P, Easton DF, Boyd NF, Vachon CM, Tamimi RM
Nature communications55303(2014 Oct)

Expression profile of MAGI2 gene as a novel biomarker in combination with major deregulated genes in prostate cancer.
Mahdian R, Nodouzi V, Asgari M, Rezaie M, Alizadeh J, Yousefi B, Shahrokh H, Abolhasani M, Nowroozi M
Molecular biology reports41(9)6125-31(2014 Sep)

Phosphorylation of the Usher syndrome 1G protein SANS controls Magi2-mediated endocytosis.
Bauß K, Knapp B, Jores P, Roepman R, Kremer H, Wijk EV, Märker T, Wolfrum U
Human molecular genetics23(15)3923-42(2014 Aug)

Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects.
Deng X, Sabino EC, Cunha-Neto E, Ribeiro AL, Ianni B, Mady C, Busch MP, Seielstad M,
PloS one8(11)e79629(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Immunohistochemistry in kidney sections from these patients revealed that mutations resulted in lack of or diminished podocyte MAGI2 expression. Our data support the finding that mutations in the MAGI2 gene are causal for congenital steroid-resistant nephrotic syndrome

MAGI-2 could represent a useful adjunct for diagnosis of prostatic adenocarcinoma
Title: MAGI-2 Is a Sensitive and Specific Marker of Prostatic Adenocarcinoma:  A Comparison With AMACR.

MAGI-2 immunoreactivity is elevated in prostate cancer and high-grade prostatic intraepithelial neoplasia compared with normal tissue, suggesting that MAGI-2 may contribute to prostate carcinogenesis.
Title: MAGI-2 in prostate cancer: an immunohistochemical study.

MAGI2, SERPINE2, and NT5C3B expression levels are associated with airway wall thickening and additionally with bronchial inflammation, emphysema, and lung function, respectively, all features of chronic obstructive pulmonary disease.
Title: Novel genes for airway wall thickness identified with combined genome-wide association and expression analyses.

The expression of MAGI2 mRNA was significantly down-regulated in PC3, LNCaP and DU-145 PCa cell lines.
Title: Expression profile of MAGI2 gene as a novel biomarker in combination with major deregulated genes in prostate cancer.

The following MAGI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu97638 XM_017012844.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $727.30
$1039.00
OHu97639 XM_017012845.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $727.30
$1039.00
OHu97640 XM_017012846.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $727.30
$1039.00
OHu97641 XM_017012848.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $727.30
$1039.00
OHu97642 XM_017012849.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu97643 XM_017012850.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu97644 XM_017012851.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu97645 XM_017012852.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu97634 XM_017012840.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 24-26 $811.30
$1159.00
OHu97635 XM_017012841.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 24-26 $811.30
$1159.00
OHu97636 XM_017012842.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 24-26 $811.30
$1159.00
OHu97637 XM_017012843.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 24-26 $811.30
$1159.00
OHu18616 NM_012301.3
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu41888 NM_001301128.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu63410 XM_011516718.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu63411 XM_011516719.2
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu63411 XM_017012847.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu63411 XM_011516720.2
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00
OHu63416 XM_011516726.2
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
OHu63417 XM_011516728.1
Latest version!
Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu97638
Clone ID Related Accession (Same CDS sequence) XM_017012844.1
Accession Version XM_017012844.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGACGAA
CCCGCCCCCT GGAGTTCTCC CGCTGCCGCC GCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
CTGGACGACG GCCTCGCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
CACCAGATAA GCCCAGGCCC AACTTGGGAT ATCAAACGGG AACACGACGT TAGGAAACCA
AAGGAGCTTT CAGCCTGCGG CCAGAAGAAG CAGCGCCTCG GGGAGCAGAG GGAGCGCTCG
GCGAGTCCGC AGAGGGCCGC GCGGCCGAGG CTCGAGGAGG CGCCCGGCGG CCAGGGGCGG
CCCGAGGCCG GCAGGCCCGC CTCGGAGGCC AGGGCGCCCG GGCTCGCGGC GGCAGACGCG
GCGGACGCGG CGCGGGCGGG CGGGAAGGAG GCGCCCCGTG CGGCGGCGGG CTCCGAGCTC
TGCCGGCGCG AAGGCCCGGG GGCTGCGCCG GCGTTTGCCG GCCCGGGCGG CGGCGGCAGC
GGCGCGCTGG AGGCCGAGGG CAGGGCGGGT GCGCGCGCGG GTCCCCGGCC GGGCCCGCGA
CCCCCGGGGG GCGCCCCGGC GCGCAAGGCC GCCGTCGCGC CGGGGCCCTG GAAGGTGCCG
GGTTCTGACA AGCTGCCCAG CGTCCTCAAA CCCGGCGCCT CGGCCGCCAG CAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868333.1
CDS486..4982
Translation

Target ORF information:

RefSeq Version XM_017012844.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012844.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGACGAA
CCCGCCCCCT GGAGTTCTCC CGCTGCCGCC GCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
CTGGACGACG GCCTCGCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
CACCAGATAA GCCCAGGCCC AACTTGGGAT ATCAAACGGG AACACGACGT TAGGAAACCA
AAGGAGCTTT CAGCCTGCGG CCAGAAGAAG CAGCGCCTCG GGGAGCAGAG GGAGCGCTCG
GCGAGTCCGC AGAGGGCCGC GCGGCCGAGG CTCGAGGAGG CGCCCGGCGG CCAGGGGCGG
CCCGAGGCCG GCAGGCCCGC CTCGGAGGCC AGGGCGCCCG GGCTCGCGGC GGCAGACGCG
GCGGACGCGG CGCGGGCGGG CGGGAAGGAG GCGCCCCGTG CGGCGGCGGG CTCCGAGCTC
TGCCGGCGCG AAGGCCCGGG GGCTGCGCCG GCGTTTGCCG GCCCGGGCGG CGGCGGCAGC
GGCGCGCTGG AGGCCGAGGG CAGGGCGGGT GCGCGCGCGG GTCCCCGGCC GGGCCCGCGA
CCCCCGGGGG GCGCCCCGGC GCGCAAGGCC GCCGTCGCGC CGGGGCCCTG GAAGGTGCCG
GGTTCTGACA AGCTGCCCAG CGTCCTCAAA CCCGGCGCCT CGGCCGCCAG CAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97639
Clone ID Related Accession (Same CDS sequence) XM_017012845.1
Accession Version XM_017012845.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAAACAAGT GCCACCCAGG
ACCAGTTTTC GAATGGATTC CTCTGGTCCA GATTATAAGG AATTGGATGT TCATCTTCGG
AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
TTGATTGGAG CTGTCATTGC CATGGGCTCA GCCGACAGAG ATGGCCGCCT TCACCCAGGA
GATGAGCTTG TGTATGTTGA TGGGATTCCA GTAGCCGGCA AAACCCACCG CTATGTCATC
GACCTCATGC ACCACGCAGC CCGCAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
CTATGTGGAG GGGAGCCCTG CCCAGAGAAC GGGAGAAGTC CAGGCTCTGT ATCCACCCAC
CACAGCTCTC CACGCAGTGA CTACGCAACC TACACCAACA GCAACCACGC TGCCCCCAGT
AGCAATGCCT CTCCCCCTGA AGGCTTCGCC TCCCACAGCC TGCAGACCAG TGATGTGGTC
ATTCACCGCA AAGAGAATGA GGGCTTCGGC TTTGTCATCA TCAGCTCCCT GAACAGGCCT
GAGTCTGGAT CCACTATAAC TGTGCCCCAT AAAATCGGAC GCATCATTGA TGGGAGTCCT
GCAGATCGCT GTGCAAAACT AAAAGTGGGA GACCGGATCC TAGCAGTGAA TGGCCAGTCT
ATCATCAACA TGCCTCACGC TGACATCGTG AAGCTCATCA AGGATGCAGG TCTTAGTGTC
ACCCTTCGCA TCATTCCTCA GGAGGAGCTC AACAGCCCCA CCTCGGCACC CAGCTCAGAG
AAGCAGAGTC CCATGGCGCA GCAGAGTCCC CTGGCACAGC AGAGTCCCCT GGCCCAGCCA
AGCCCAGCCA CCCCCAACAG CCCCATCGCC CAGCCAGCAC CACCTCAACC ACTTCAGCTG
CAAGGACACG AAAATAGTTA CAGGTCGGAA GTGAAAGCAA GGCAAGATGT GAAACCAGAC
ATCCGACAGC CTCCATTCAC AGACTACAGG CAGCCCCCGC TGGATTACAG GCAACCCCCA
GGAGGGGACT ACCAGCAGCC CCCACCCTTG GACTACAGGC AGCCTCCCCT ACTGGACTAC
AGGCAGCACT CCCCCGACAC CAGGCAGTAC CCTCTGTCGG ACTACAGACA ACCCCAGGAT
TTTGATTATT TCACTGTGGA CATGGAGAAA GGAGCCAAAG GATTTGGATT CAGCATTCGT
GGAGGAAGGG AATACAAAAT GGATTTGTAT GTGTTGAGAC TGGCAGAAGA TGGACCAGCA
ATAAGGAATG GGAGGATGAG GGTAGGAGAT CAAATCATTG AAATCAATGG GGAAAGCACA
AGGGACATGA CACATGCCAG AGCAATAGAA CTCATCAAAT CTGGAGGAAG ACGAGTGAGG
CTGCTGCTCA AGAGAGGCAC GGGACAGGTC CCAGAATATG GAATGGTACC TTCCAGTCTC
TCCATGTGCA TGAAAAGTGA CAAGCATGGG TCCCCATATT TCTACTTATT GGGCCACCCT
AAAGACACGG AAAGATCCAA ACTGGCTAGA ACTGACAATA GGAAGGACGA ACCCGCCCCC
TGGAGTTCTC CCGCTGCCGC CGCCCCAGGT CTGCCGGAAG TAGGCGTCTC CCTGGACGAC
GGCCTCGCTC CATTCTCTCC ATCACATCCA GCCCCACCCT CCGACCCTTC CCACCAGATA
AGCCCAGGCC CAACTTGGGA TATCAAACGG GAACACGACG TTAGGAAACC AAAGGAGCTT
TCAGCCTGCG GCCAGAAGAA GCAGCGCCTC GGGGAGCAGA GGGAGCGCTC GGCGAGTCCG
CAGAGGGCCG CGCGGCCGAG GCTCGAGGAG GCGCCCGGCG GCCAGGGGCG GCCCGAGGCC
GGCAGGCCCG CCTCGGAGGC CAGGGCGCCC GGGCTCGCGG CGGCAGACGC GGCGGACGCG
GCGCGGGCGG GCGGGAAGGA GGCGCCCCGT GCGGCGGCGG GCTCCGAGCT CTGCCGGCGC
GAAGGCCCGG GGGCTGCGCC GGCGTTTGCC GGCCCGGGCG GCGGCGGCAG CGGCGCGCTG
GAGGCCGAGG GCAGGGCGGG TGCGCGCGCG GGTCCCCGGC CGGGCCCGCG ACCCCCGGGG
GGCGCCCCGG CGCGCAAGGC CGCCGTCGCG CCGGGGCCCT GGAAGGTGCC GGGTTCTGAC
AAGCTGCCCA GCGTCCTCAA ACCCGGCGCC TCGGCCGCCA GCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868334.1
CDS486..4973
Translation

Target ORF information:

RefSeq Version XM_017012845.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012845.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAAACAAGT GCCACCCAGG
ACCAGTTTTC GAATGGATTC CTCTGGTCCA GATTATAAGG AATTGGATGT TCATCTTCGG
AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
TTGATTGGAG CTGTCATTGC CATGGGCTCA GCCGACAGAG ATGGCCGCCT TCACCCAGGA
GATGAGCTTG TGTATGTTGA TGGGATTCCA GTAGCCGGCA AAACCCACCG CTATGTCATC
GACCTCATGC ACCACGCAGC CCGCAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
CTATGTGGAG GGGAGCCCTG CCCAGAGAAC GGGAGAAGTC CAGGCTCTGT ATCCACCCAC
CACAGCTCTC CACGCAGTGA CTACGCAACC TACACCAACA GCAACCACGC TGCCCCCAGT
AGCAATGCCT CTCCCCCTGA AGGCTTCGCC TCCCACAGCC TGCAGACCAG TGATGTGGTC
ATTCACCGCA AAGAGAATGA GGGCTTCGGC TTTGTCATCA TCAGCTCCCT GAACAGGCCT
GAGTCTGGAT CCACTATAAC TGTGCCCCAT AAAATCGGAC GCATCATTGA TGGGAGTCCT
GCAGATCGCT GTGCAAAACT AAAAGTGGGA GACCGGATCC TAGCAGTGAA TGGCCAGTCT
ATCATCAACA TGCCTCACGC TGACATCGTG AAGCTCATCA AGGATGCAGG TCTTAGTGTC
ACCCTTCGCA TCATTCCTCA GGAGGAGCTC AACAGCCCCA CCTCGGCACC CAGCTCAGAG
AAGCAGAGTC CCATGGCGCA GCAGAGTCCC CTGGCACAGC AGAGTCCCCT GGCCCAGCCA
AGCCCAGCCA CCCCCAACAG CCCCATCGCC CAGCCAGCAC CACCTCAACC ACTTCAGCTG
CAAGGACACG AAAATAGTTA CAGGTCGGAA GTGAAAGCAA GGCAAGATGT GAAACCAGAC
ATCCGACAGC CTCCATTCAC AGACTACAGG CAGCCCCCGC TGGATTACAG GCAACCCCCA
GGAGGGGACT ACCAGCAGCC CCCACCCTTG GACTACAGGC AGCCTCCCCT ACTGGACTAC
AGGCAGCACT CCCCCGACAC CAGGCAGTAC CCTCTGTCGG ACTACAGACA ACCCCAGGAT
TTTGATTATT TCACTGTGGA CATGGAGAAA GGAGCCAAAG GATTTGGATT CAGCATTCGT
GGAGGAAGGG AATACAAAAT GGATTTGTAT GTGTTGAGAC TGGCAGAAGA TGGACCAGCA
ATAAGGAATG GGAGGATGAG GGTAGGAGAT CAAATCATTG AAATCAATGG GGAAAGCACA
AGGGACATGA CACATGCCAG AGCAATAGAA CTCATCAAAT CTGGAGGAAG ACGAGTGAGG
CTGCTGCTCA AGAGAGGCAC GGGACAGGTC CCAGAATATG GAATGGTACC TTCCAGTCTC
TCCATGTGCA TGAAAAGTGA CAAGCATGGG TCCCCATATT TCTACTTATT GGGCCACCCT
AAAGACACGG AAAGATCCAA ACTGGCTAGA ACTGACAATA GGAAGGACGA ACCCGCCCCC
TGGAGTTCTC CCGCTGCCGC CGCCCCAGGT CTGCCGGAAG TAGGCGTCTC CCTGGACGAC
GGCCTCGCTC CATTCTCTCC ATCACATCCA GCCCCACCCT CCGACCCTTC CCACCAGATA
AGCCCAGGCC CAACTTGGGA TATCAAACGG GAACACGACG TTAGGAAACC AAAGGAGCTT
TCAGCCTGCG GCCAGAAGAA GCAGCGCCTC GGGGAGCAGA GGGAGCGCTC GGCGAGTCCG
CAGAGGGCCG CGCGGCCGAG GCTCGAGGAG GCGCCCGGCG GCCAGGGGCG GCCCGAGGCC
GGCAGGCCCG CCTCGGAGGC CAGGGCGCCC GGGCTCGCGG CGGCAGACGC GGCGGACGCG
GCGCGGGCGG GCGGGAAGGA GGCGCCCCGT GCGGCGGCGG GCTCCGAGCT CTGCCGGCGC
GAAGGCCCGG GGGCTGCGCC GGCGTTTGCC GGCCCGGGCG GCGGCGGCAG CGGCGCGCTG
GAGGCCGAGG GCAGGGCGGG TGCGCGCGCG GGTCCCCGGC CGGGCCCGCG ACCCCCGGGG
GGCGCCCCGG CGCGCAAGGC CGCCGTCGCG CCGGGGCCCT GGAAGGTGCC GGGTTCTGAC
AAGCTGCCCA GCGTCCTCAA ACCCGGCGCC TCGGCCGCCA GCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97640
Clone ID Related Accession (Same CDS sequence) XM_017012846.1
Accession Version XM_017012846.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCG TCCAGATTAT
AAGGAATTGG ATGTTCATCT TCGGAGGATG GAGTCTGGAT TTGGCTTCAG AATCCTCGGG
GGAGATGAGC CTGGACAGCC TATTTTGATT GGAGCTGTCA TTGCCATGGG CTCAGCCGAC
AGAGATGGCC GCCTTCACCC AGGAGATGAG CTTGTGTATG TTGATGGGAT TCCAGTAGCC
GGCAAAACCC ACCGCTATGT CATCGACCTC ATGCACCACG CAGCCCGCAA TGGGCAGGTC
AACCTCACTG TGAGAAGAAA GGTGCTATGT GGAGGGGAGC CCTGCCCAGA GAACGGGAGA
AGTCCAGGCT CTGTATCCAC CCACCACAGC TCTCCACGCA GTGACTACGC AACCTACACC
AACAGCAACC ACGCTGCCCC CAGTAGCAAT GCCTCTCCCC CTGAAGGCTT CGCCTCCCAC
AGCCTGCAGA CCAGTGATGT GGTCATTCAC CGCAAAGAGA ATGAGGGCTT CGGCTTTGTC
ATCATCAGCT CCCTGAACAG GCCTGAGTCT GGATCCACTA TAACTGTGCC CCATAAAATC
GGACGCATCA TTGATGGGAG TCCTGCAGAT CGCTGTGCAA AACTAAAAGT GGGAGACCGG
ATCCTAGCAG TGAATGGCCA GTCTATCATC AACATGCCTC ACGCTGACAT CGTGAAGCTC
ATCAAGGATG CAGGTCTTAG TGTCACCCTT CGCATCATTC CTCAGGAGGA GCTCAACAGC
CCCACCTCGG CACCCAGCTC AGAGAAGCAG AGTCCCATGG CGCAGCAGAG TCCCCTGGCA
CAGCAGAGTC CCCTGGCCCA GCCAAGCCCA GCCACCCCCA ACAGCCCCAT CGCCCAGCCA
GCACCACCTC AACCACTTCA GCTGCAAGGA CACGAAAATA GTTACAGGTC GGAAGTGAAA
GCAAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACAGACTA CAGGCAGCCC
CCGCTGGATT ACAGGCAACC CCCAGGAGGG GACTACCAGC AGCCCCCACC CTTGGACTAC
AGGCAGCCTC CCCTACTGGA CTACAGGCAG CACTCCCCCG ACACCAGGCA GTACCCTCTG
TCGGACTACA GACAACCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAATACA AAATGGATTT GTATGTGTTG
AGACTGGCAG AAGATGGACC AGCAATAAGG AATGGGAGGA TGAGGGTAGG AGATCAAATC
ATTGAAATCA ATGGGGAAAG CACAAGGGAC ATGACACATG CCAGAGCAAT AGAACTCATC
AAATCTGGAG GAAGACGAGT GAGGCTGCTG CTCAAGAGAG GCACGGGACA GGTCCCAGAA
TATGGAATGG TACCTTCCAG TCTCTCCATG TGCATGAAAA GTGACAAGCA TGGGTCCCCA
TATTTCTACT TATTGGGCCA CCCTAAAGAC ACGGAAAGAT CCAAACTGGC TAGAACTGAC
AATAGGAAGG ACGAACCCGC CCCCTGGAGT TCTCCCGCTG CCGCCGCCCC AGGTCTGCCG
GAAGTAGGCG TCTCCCTGGA CGACGGCCTC GCTCCATTCT CTCCATCACA TCCAGCCCCA
CCCTCCGACC CTTCCCACCA GATAAGCCCA GGCCCAACTT GGGATATCAA ACGGGAACAC
GACGTTAGGA AACCAAAGGA GCTTTCAGCC TGCGGCCAGA AGAAGCAGCG CCTCGGGGAG
CAGAGGGAGC GCTCGGCGAG TCCGCAGAGG GCCGCGCGGC CGAGGCTCGA GGAGGCGCCC
GGCGGCCAGG GGCGGCCCGA GGCCGGCAGG CCCGCCTCGG AGGCCAGGGC GCCCGGGCTC
GCGGCGGCAG ACGCGGCGGA CGCGGCGCGG GCGGGCGGGA AGGAGGCGCC CCGTGCGGCG
GCGGGCTCCG AGCTCTGCCG GCGCGAAGGC CCGGGGGCTG CGCCGGCGTT TGCCGGCCCG
GGCGGCGGCG GCAGCGGCGC GCTGGAGGCC GAGGGCAGGG CGGGTGCGCG CGCGGGTCCC
CGGCCGGGCC CGCGACCCCC GGGGGGCGCC CCGGCGCGCA AGGCCGCCGT CGCGCCGGGG
CCCTGGAAGG TGCCGGGTTC TGACAAGCTG CCCAGCGTCC TCAAACCCGG CGCCTCGGCC
GCCAGCAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868335.1
CDS486..4937
Translation

Target ORF information:

RefSeq Version XM_017012846.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012846.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCG TCCAGATTAT
AAGGAATTGG ATGTTCATCT TCGGAGGATG GAGTCTGGAT TTGGCTTCAG AATCCTCGGG
GGAGATGAGC CTGGACAGCC TATTTTGATT GGAGCTGTCA TTGCCATGGG CTCAGCCGAC
AGAGATGGCC GCCTTCACCC AGGAGATGAG CTTGTGTATG TTGATGGGAT TCCAGTAGCC
GGCAAAACCC ACCGCTATGT CATCGACCTC ATGCACCACG CAGCCCGCAA TGGGCAGGTC
AACCTCACTG TGAGAAGAAA GGTGCTATGT GGAGGGGAGC CCTGCCCAGA GAACGGGAGA
AGTCCAGGCT CTGTATCCAC CCACCACAGC TCTCCACGCA GTGACTACGC AACCTACACC
AACAGCAACC ACGCTGCCCC CAGTAGCAAT GCCTCTCCCC CTGAAGGCTT CGCCTCCCAC
AGCCTGCAGA CCAGTGATGT GGTCATTCAC CGCAAAGAGA ATGAGGGCTT CGGCTTTGTC
ATCATCAGCT CCCTGAACAG GCCTGAGTCT GGATCCACTA TAACTGTGCC CCATAAAATC
GGACGCATCA TTGATGGGAG TCCTGCAGAT CGCTGTGCAA AACTAAAAGT GGGAGACCGG
ATCCTAGCAG TGAATGGCCA GTCTATCATC AACATGCCTC ACGCTGACAT CGTGAAGCTC
ATCAAGGATG CAGGTCTTAG TGTCACCCTT CGCATCATTC CTCAGGAGGA GCTCAACAGC
CCCACCTCGG CACCCAGCTC AGAGAAGCAG AGTCCCATGG CGCAGCAGAG TCCCCTGGCA
CAGCAGAGTC CCCTGGCCCA GCCAAGCCCA GCCACCCCCA ACAGCCCCAT CGCCCAGCCA
GCACCACCTC AACCACTTCA GCTGCAAGGA CACGAAAATA GTTACAGGTC GGAAGTGAAA
GCAAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACAGACTA CAGGCAGCCC
CCGCTGGATT ACAGGCAACC CCCAGGAGGG GACTACCAGC AGCCCCCACC CTTGGACTAC
AGGCAGCCTC CCCTACTGGA CTACAGGCAG CACTCCCCCG ACACCAGGCA GTACCCTCTG
TCGGACTACA GACAACCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAATACA AAATGGATTT GTATGTGTTG
AGACTGGCAG AAGATGGACC AGCAATAAGG AATGGGAGGA TGAGGGTAGG AGATCAAATC
ATTGAAATCA ATGGGGAAAG CACAAGGGAC ATGACACATG CCAGAGCAAT AGAACTCATC
AAATCTGGAG GAAGACGAGT GAGGCTGCTG CTCAAGAGAG GCACGGGACA GGTCCCAGAA
TATGGAATGG TACCTTCCAG TCTCTCCATG TGCATGAAAA GTGACAAGCA TGGGTCCCCA
TATTTCTACT TATTGGGCCA CCCTAAAGAC ACGGAAAGAT CCAAACTGGC TAGAACTGAC
AATAGGAAGG ACGAACCCGC CCCCTGGAGT TCTCCCGCTG CCGCCGCCCC AGGTCTGCCG
GAAGTAGGCG TCTCCCTGGA CGACGGCCTC GCTCCATTCT CTCCATCACA TCCAGCCCCA
CCCTCCGACC CTTCCCACCA GATAAGCCCA GGCCCAACTT GGGATATCAA ACGGGAACAC
GACGTTAGGA AACCAAAGGA GCTTTCAGCC TGCGGCCAGA AGAAGCAGCG CCTCGGGGAG
CAGAGGGAGC GCTCGGCGAG TCCGCAGAGG GCCGCGCGGC CGAGGCTCGA GGAGGCGCCC
GGCGGCCAGG GGCGGCCCGA GGCCGGCAGG CCCGCCTCGG AGGCCAGGGC GCCCGGGCTC
GCGGCGGCAG ACGCGGCGGA CGCGGCGCGG GCGGGCGGGA AGGAGGCGCC CCGTGCGGCG
GCGGGCTCCG AGCTCTGCCG GCGCGAAGGC CCGGGGGCTG CGCCGGCGTT TGCCGGCCCG
GGCGGCGGCG GCAGCGGCGC GCTGGAGGCC GAGGGCAGGG CGGGTGCGCG CGCGGGTCCC
CGGCCGGGCC CGCGACCCCC GGGGGGCGCC CCGGCGCGCA AGGCCGCCGT CGCGCCGGGG
CCCTGGAAGG TGCCGGGTTC TGACAAGCTG CCCAGCGTCC TCAAACCCGG CGCCTCGGCC
GCCAGCAGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97641
Clone ID Related Accession (Same CDS sequence) XM_017012848.1
Accession Version XM_017012848.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGAAAA ACCACTCTTC ACCCGGGATG CATCCCAGTT GAAGGGAACA
TTCCTCAGCA CCACCCTAAA AAAGAGCAAC ATGGGCTTTG GATTTACCAT CATTGGTGGA
GACGAGCCTG ATGAGTTTCT GCAGGTGAAA AGTGTGATTC CGGATGGGCC TGCAGCACAG
GATGGAAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
CACACTCATG CAGATGTTGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
CTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGACCCTGC TAACAGCATG
GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACACAAC
TATGAAACAT ATTTGGAGTA CATTTCTCGG ACCTCACAGT CAGTTCCAGA TATAACAGAT
CGGCCGCCTC ATTCTCTGCA CTCCATGCCA ACTGATGGTC AGCTAGACGG CACGTATCCA
CCGCCCGTCC ATGATGACAA TGTGTCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGCTTCGGCT TCACTATTGC CGACAGTCCT
ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
GACCTCATTG TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC AGAAGTAGTG
GATATACTTA AGGACTGTCC CATTGGAAGT GAAACTTCTT TGATTATCCA TCGAGGAGGT
TTCTTTTCTC CATGGAAAAC TCCAAAGCCT ATAATGGACC GATGGGAGAA TCAAGGCAGT
CCTCAAACGA GTTTATCTGC TCCGGCCATA CCGCAGAACC TGCCCTTCCC ACCTGCCCTT
CACAGGAGCT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CACGGAAGCC TGATCCATAT
GAGCTCTACG AGAAATCTAG GGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCCAGGACC
AGTTTTCGAA TGGATTCCTC TGGTCCAGAT TATAAGGAAT TGGATGTTCA TCTTCGGAGG
ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
ATTGGAGCTG TCATTGCCAT GGGCTCAGCC GACAGAGATG GCCGCCTTCA CCCAGGAGAT
GAGCTTGTGT ATGTTGATGG GATTCCAGTA GCCGGCAAAA CCCACCGCTA TGTCATCGAC
CTCATGCACC ACGCAGCCCG CAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
TGTGGAGGGG AGCCCTGCCC AGAGAACGGG AGAAGTCCAG GCTCTGTATC CACCCACCAC
AGCTCTCCAC GCAGTGACTA CGCAACCTAC ACCAACAGCA ACCACGCTGC CCCCAGTAGC
AATGCCTCTC CCCCTGAAGG CTTCGCCTCC CACAGCCTGC AGACCAGTGA TGTGGTCATT
CACCGCAAAG AGAATGAGGG CTTCGGCTTT GTCATCATCA GCTCCCTGAA CAGGCCTGAG
TCTGGATCCA CTATAACTGT GCCCCATAAA ATCGGACGCA TCATTGATGG GAGTCCTGCA
GATCGCTGTG CAAAACTAAA AGTGGGAGAC CGGATCCTAG CAGTGAATGG CCAGTCTATC
ATCAACATGC CTCACGCTGA CATCGTGAAG CTCATCAAGG ATGCAGGTCT TAGTGTCACC
CTTCGCATCA TTCCTCAGGA GGAGCTCAAC AGCCCCACCT CGGCACCCAG CTCAGAGAAG
CAGAGTCCCA TGGCGCAGCA GAGTCCCCTG GCACAGCAGA GTCCCCTGGC CCAGCCAAGC
CCAGCCACCC CCAACAGCCC CATCGCCCAG CCAGCACCAC CTCAACCACT TCAGCTGCAA
GGACACGAAA ATAGTTACAG GTCGGAAGTG AAAGCAAGGC AAGATGTGAA ACCAGACATC
CGACAGCCTC CATTCACAGA CTACAGGCAG CCCCCGCTGG ATTACAGGCA ACCCCCAGGA
GGGGACTACC AGCAGCCCCC ACCCTTGGAC TACAGGCAGC CTCCCCTACT GGACTACAGG
CAGCACTCCC CCGACACCAG GCAGTACCCT CTGTCGGACT ACAGACAACC CCAGGATTTT
GATTATTTCA CTGTGGACAT GGAGAAAGGA GCCAAAGGAT TTGGATTCAG CATTCGTGGA
GGAAGGGAAT ACAAAATGGA TTTGTATGTG TTGAGACTGG CAGAAGATGG ACCAGCAATA
AGGAATGGGA GGATGAGGGT AGGAGATCAA ATCATTGAAA TCAATGGGGA AAGCACAAGG
GACATGACAC ATGCCAGAGC AATAGAACTC ATCAAATCTG GAGGAAGACG AGTGAGGCTG
CTGCTCAAGA GAGGCACGGG ACAGGTCCCA GAATATGGAA TGGTACCTTC CAGTCTCTCC
ATGTGCATGA AAAGTGACAA GCATGGGTCC CCATATTTCT ACTTATTGGG CCACCCTAAA
GACACGGAAA GATCCAAACT GGCTAGAACT GACAATAGGA AGGACGAACC CGCCCCCTGG
AGTTCTCCCG CTGCCGCCGC CCCAGGTCTG CCGGAAGTAG GCGTCTCCCT GGACGACGGC
CTCGCTCCAT TCTCTCCATC ACATCCAGCC CCACCCTCCG ACCCTTCCCA CCAGATAAGC
CCAGGCCCAA CTTGGGATAT CAAACGGGAA CACGACGTTA GGAAACCAAA GGAGCTTTCA
GCCTGCGGCC AGAAGAAGCA GCGCCTCGGG GAGCAGAGGG AGCGCTCGGC GAGTCCGCAG
AGGGCCGCGC GGCCGAGGCT CGAGGAGGCG CCCGGCGGCC AGGGGCGGCC CGAGGCCGGC
AGGCCCGCCT CGGAGGCCAG GGCGCCCGGG CTCGCGGCGG CAGACGCGGC GGACGCGGCG
CGGGCGGGCG GGAAGGAGGC GCCCCGTGCG GCGGCGGGCT CCGAGCTCTG CCGGCGCGAA
GGCCCGGGGG CTGCGCCGGC GTTTGCCGGC CCGGGCGGCG GCGGCAGCGG CGCGCTGGAG
GCCGAGGGCA GGGCGGGTGC GCGCGCGGGT CCCCGGCCGG GCCCGCGACC CCCGGGGGGC
GCCCCGGCGC GCAAGGCCGC CGTCGCGCCG GGGCCCTGGA AGGTGCCGGG TTCTGACAAG
CTGCCCAGCG TCCTCAAACC CGGCGCCTCG GCCGCCAGCA GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868337.1
CDS4260..8264
Translation

Target ORF information:

RefSeq Version XM_017012848.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012848.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGAAAA ACCACTCTTC ACCCGGGATG CATCCCAGTT GAAGGGAACA
TTCCTCAGCA CCACCCTAAA AAAGAGCAAC ATGGGCTTTG GATTTACCAT CATTGGTGGA
GACGAGCCTG ATGAGTTTCT GCAGGTGAAA AGTGTGATTC CGGATGGGCC TGCAGCACAG
GATGGAAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
CACACTCATG CAGATGTTGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
CTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGACCCTGC TAACAGCATG
GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACACAAC
TATGAAACAT ATTTGGAGTA CATTTCTCGG ACCTCACAGT CAGTTCCAGA TATAACAGAT
CGGCCGCCTC ATTCTCTGCA CTCCATGCCA ACTGATGGTC AGCTAGACGG CACGTATCCA
CCGCCCGTCC ATGATGACAA TGTGTCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGCTTCGGCT TCACTATTGC CGACAGTCCT
ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
GACCTCATTG TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC AGAAGTAGTG
GATATACTTA AGGACTGTCC CATTGGAAGT GAAACTTCTT TGATTATCCA TCGAGGAGGT
TTCTTTTCTC CATGGAAAAC TCCAAAGCCT ATAATGGACC GATGGGAGAA TCAAGGCAGT
CCTCAAACGA GTTTATCTGC TCCGGCCATA CCGCAGAACC TGCCCTTCCC ACCTGCCCTT
CACAGGAGCT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CACGGAAGCC TGATCCATAT
GAGCTCTACG AGAAATCTAG GGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCCAGGACC
AGTTTTCGAA TGGATTCCTC TGGTCCAGAT TATAAGGAAT TGGATGTTCA TCTTCGGAGG
ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
ATTGGAGCTG TCATTGCCAT GGGCTCAGCC GACAGAGATG GCCGCCTTCA CCCAGGAGAT
GAGCTTGTGT ATGTTGATGG GATTCCAGTA GCCGGCAAAA CCCACCGCTA TGTCATCGAC
CTCATGCACC ACGCAGCCCG CAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
TGTGGAGGGG AGCCCTGCCC AGAGAACGGG AGAAGTCCAG GCTCTGTATC CACCCACCAC
AGCTCTCCAC GCAGTGACTA CGCAACCTAC ACCAACAGCA ACCACGCTGC CCCCAGTAGC
AATGCCTCTC CCCCTGAAGG CTTCGCCTCC CACAGCCTGC AGACCAGTGA TGTGGTCATT
CACCGCAAAG AGAATGAGGG CTTCGGCTTT GTCATCATCA GCTCCCTGAA CAGGCCTGAG
TCTGGATCCA CTATAACTGT GCCCCATAAA ATCGGACGCA TCATTGATGG GAGTCCTGCA
GATCGCTGTG CAAAACTAAA AGTGGGAGAC CGGATCCTAG CAGTGAATGG CCAGTCTATC
ATCAACATGC CTCACGCTGA CATCGTGAAG CTCATCAAGG ATGCAGGTCT TAGTGTCACC
CTTCGCATCA TTCCTCAGGA GGAGCTCAAC AGCCCCACCT CGGCACCCAG CTCAGAGAAG
CAGAGTCCCA TGGCGCAGCA GAGTCCCCTG GCACAGCAGA GTCCCCTGGC CCAGCCAAGC
CCAGCCACCC CCAACAGCCC CATCGCCCAG CCAGCACCAC CTCAACCACT TCAGCTGCAA
GGACACGAAA ATAGTTACAG GTCGGAAGTG AAAGCAAGGC AAGATGTGAA ACCAGACATC
CGACAGCCTC CATTCACAGA CTACAGGCAG CCCCCGCTGG ATTACAGGCA ACCCCCAGGA
GGGGACTACC AGCAGCCCCC ACCCTTGGAC TACAGGCAGC CTCCCCTACT GGACTACAGG
CAGCACTCCC CCGACACCAG GCAGTACCCT CTGTCGGACT ACAGACAACC CCAGGATTTT
GATTATTTCA CTGTGGACAT GGAGAAAGGA GCCAAAGGAT TTGGATTCAG CATTCGTGGA
GGAAGGGAAT ACAAAATGGA TTTGTATGTG TTGAGACTGG CAGAAGATGG ACCAGCAATA
AGGAATGGGA GGATGAGGGT AGGAGATCAA ATCATTGAAA TCAATGGGGA AAGCACAAGG
GACATGACAC ATGCCAGAGC AATAGAACTC ATCAAATCTG GAGGAAGACG AGTGAGGCTG
CTGCTCAAGA GAGGCACGGG ACAGGTCCCA GAATATGGAA TGGTACCTTC CAGTCTCTCC
ATGTGCATGA AAAGTGACAA GCATGGGTCC CCATATTTCT ACTTATTGGG CCACCCTAAA
GACACGGAAA GATCCAAACT GGCTAGAACT GACAATAGGA AGGACGAACC CGCCCCCTGG
AGTTCTCCCG CTGCCGCCGC CCCAGGTCTG CCGGAAGTAG GCGTCTCCCT GGACGACGGC
CTCGCTCCAT TCTCTCCATC ACATCCAGCC CCACCCTCCG ACCCTTCCCA CCAGATAAGC
CCAGGCCCAA CTTGGGATAT CAAACGGGAA CACGACGTTA GGAAACCAAA GGAGCTTTCA
GCCTGCGGCC AGAAGAAGCA GCGCCTCGGG GAGCAGAGGG AGCGCTCGGC GAGTCCGCAG
AGGGCCGCGC GGCCGAGGCT CGAGGAGGCG CCCGGCGGCC AGGGGCGGCC CGAGGCCGGC
AGGCCCGCCT CGGAGGCCAG GGCGCCCGGG CTCGCGGCGG CAGACGCGGC GGACGCGGCG
CGGGCGGGCG GGAAGGAGGC GCCCCGTGCG GCGGCGGGCT CCGAGCTCTG CCGGCGCGAA
GGCCCGGGGG CTGCGCCGGC GTTTGCCGGC CCGGGCGGCG GCGGCAGCGG CGCGCTGGAG
GCCGAGGGCA GGGCGGGTGC GCGCGCGGGT CCCCGGCCGG GCCCGCGACC CCCGGGGGGC
GCCCCGGCGC GCAAGGCCGC CGTCGCGCCG GGGCCCTGGA AGGTGCCGGG TTCTGACAAG
CTGCCCAGCG TCCTCAAACC CGGCGCCTCG GCCGCCAGCA GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97642
Clone ID Related Accession (Same CDS sequence) XM_017012849.1
Accession Version XM_017012849.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGAAAA ACCACTCTTC ACCCGGGATG CATCCCAGTT GAAGGGAACA
TTCCTCAGCA CCACCCTAAA AAAGAGCAAC ATGGGCTTTG GATTTACCAT CATTGGTGGA
GACGAGCCTG ATGAGTTTCT GCAGGTGAAA AGTGTGATTC CGGATGGGCC TGCAGCACAG
GATGGAAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
CACACTCATG CAGATGTTGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
CTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGACCCTGC TAACAGCATG
GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACACAAC
TATGAAACAT ATTTGGAGTA CATTTCTCGG ACCTCACAGT CAGTTCCAGA TATAACAGAT
CGGCCGCCTC ATTCTCTGCA CTCCATGCCA ACTGATGGTC AGCTAGACGG CACGTATCCA
CCGCCCGTCC ATGATGACAA TGTGTCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGCTTCGGCT TCACTATTGC CGACAGTCCT
ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
GACCTCATTG TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC AGAAGTAGTG
GATATACTTA AGGACTGTCC CATTGGAAGT GAAACTTCTT TGATTATCCA TCGAGGAGGT
TTCTTTTCTC CATGGAAAAC TCCAAAGCCT ATAATGGACC GATGGGAGAA TCAAGGCAGT
CCTCAAACGA GTTTATCTGC TCCGGCCATA CCGCAGAACC TGCCCTTCCC ACCTGCCCTT
CACAGGAGCT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CACGGAAGCC TGATCCATAT
GAGCTCTACG AGAAATCTAG GGCCATTTAT GAAAAACAAG TGCCACCCAG GACCAGTTTT
CGAATGGATT CCTCTGGTCC AGATTATAAG GAATTGGATG TTCATCTTCG GAGGATGGAG
TCTGGATTTG GCTTCAGAAT CCTCGGGGGA GATGAGCCTG GACAGCCTAT TTTGATTGGA
GCTGTCATTG CCATGGGCTC AGCCGACAGA GATGGCCGCC TTCACCCAGG AGATGAGCTT
GTGTATGTTG ATGGGATTCC AGTAGCCGGC AAAACCCACC GCTATGTCAT CGACCTCATG
CACCACGCAG CCCGCAATGG GCAGGTCAAC CTCACTGTGA GAAGAAAGGT GCTATGTGGA
GGGGAGCCCT GCCCAGAGAA CGGGAGAAGT CCAGGCTCTG TATCCACCCA CCACAGCTCT
CCACGCAGTG ACTACGCAAC CTACACCAAC AGCAACCACG CTGCCCCCAG TAGCAATGCC
TCTCCCCCTG AAGGCTTCGC CTCCCACAGC CTGCAGACCA GTGATGTGGT CATTCACCGC
AAAGAGAATG AGGGCTTCGG CTTTGTCATC ATCAGCTCCC TGAACAGGCC TGAGTCTGGA
TCCACTATAA CTGTGCCCCA TAAAATCGGA CGCATCATTG ATGGGAGTCC TGCAGATCGC
TGTGCAAAAC TAAAAGTGGG AGACCGGATC CTAGCAGTGA ATGGCCAGTC TATCATCAAC
ATGCCTCACG CTGACATCGT GAAGCTCATC AAGGATGCAG GTCTTAGTGT CACCCTTCGC
ATCATTCCTC AGGAGGAGCT CAACAGCCCC ACCTCGGCAC CCAGCTCAGA GAAGCAGAGT
CCCATGGCGC AGCAGAGTCC CCTGGCACAG CAGAGTCCCC TGGCCCAGCC AAGCCCAGCC
ACCCCCAACA GCCCCATCGC CCAGCCAGCA CCACCTCAAC CACTTCAGCT GCAAGGACAC
GAAAATAGTT ACAGGTCGGA AGTGAAAGCA AGGCAAGATG TGAAACCAGA CATCCGACAG
CCTCCATTCA CAGACTACAG GCAGCCCCCG CTGGATTACA GGCAACCCCC AGGAGGGGAC
TACCAGCAGC CCCCACCCTT GGACTACAGG CAGCCTCCCC TACTGGACTA CAGGCAGCAC
TCCCCCGACA CCAGGCAGTA CCCTCTGTCG GACTACAGAC AACCCCAGGA TTTTGATTAT
TTCACTGTGG ACATGGAGAA AGGAGCCAAA GGATTTGGAT TCAGCATTCG TGGAGGAAGG
GAATACAAAA TGGATTTGTA TGTGTTGAGA CTGGCAGAAG ATGGACCAGC AATAAGGAAT
GGGAGGATGA GGGTAGGAGA TCAAATCATT GAAATCAATG GGGAAAGCAC AAGGGACATG
ACACATGCCA GAGCAATAGA ACTCATCAAA TCTGGAGGAA GACGAGTGAG GCTGCTGCTC
AAGAGAGGCA CGGGACAGGT CCCAGAATAT GGAATGGTAC CTTCCAGTCT CTCCATGTGC
ATGAAAAGTG ACAAGCATGG GTCCCCATAT TTCTACTTAT TGGGCCACCC TAAAGACACG
GAAAGATCCA AACTGGCTAG AACTGACAAT AGGAAGGACG AACCCGCCCC CTGGAGTTCT
CCCGCTGCCG CCGCCCCAGG TCTGCCGGAA GTAGGCGTCT CCCTGGACGA CGGCCTCGCT
CCATTCTCTC CATCACATCC AGCCCCACCC TCCGACCCTT CCCACCAGAT AAGCCCAGGC
CCAACTTGGG ATATCAAACG GGAACACGAC GTTAGGAAAC CAAAGGAGCT TTCAGCCTGC
GGCCAGAAGA AGCAGCGCCT CGGGGAGCAG AGGGAGCGCT CGGCGAGTCC GCAGAGGGCC
GCGCGGCCGA GGCTCGAGGA GGCGCCCGGC GGCCAGGGGC GGCCCGAGGC CGGCAGGCCC
GCCTCGGAGG CCAGGGCGCC CGGGCTCGCG GCGGCAGACG CGGCGGACGC GGCGCGGGCG
GGCGGGAAGG AGGCGCCCCG TGCGGCGGCG GGCTCCGAGC TCTGCCGGCG CGAAGGCCCG
GGGGCTGCGC CGGCGTTTGC CGGCCCGGGC GGCGGCGGCA GCGGCGCGCT GGAGGCCGAG
GGCAGGGCGG GTGCGCGCGC GGGTCCCCGG CCGGGCCCGC GACCCCCGGG GGGCGCCCCG
GCGCGCAAGG CCGCCGTCGC GCCGGGGCCC TGGAAGGTGC CGGGTTCTGA CAAGCTGCCC
AGCGTCCTCA AACCCGGCGC CTCGGCCGCC AGCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868338.1
CDS4260..8258
Translation

Target ORF information:

RefSeq Version XM_017012849.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012849.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGAAAA ACCACTCTTC ACCCGGGATG CATCCCAGTT GAAGGGAACA
TTCCTCAGCA CCACCCTAAA AAAGAGCAAC ATGGGCTTTG GATTTACCAT CATTGGTGGA
GACGAGCCTG ATGAGTTTCT GCAGGTGAAA AGTGTGATTC CGGATGGGCC TGCAGCACAG
GATGGAAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
CACACTCATG CAGATGTTGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
CTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGACCCTGC TAACAGCATG
GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACACAAC
TATGAAACAT ATTTGGAGTA CATTTCTCGG ACCTCACAGT CAGTTCCAGA TATAACAGAT
CGGCCGCCTC ATTCTCTGCA CTCCATGCCA ACTGATGGTC AGCTAGACGG CACGTATCCA
CCGCCCGTCC ATGATGACAA TGTGTCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGCTTCGGCT TCACTATTGC CGACAGTCCT
ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
GACCTCATTG TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC AGAAGTAGTG
GATATACTTA AGGACTGTCC CATTGGAAGT GAAACTTCTT TGATTATCCA TCGAGGAGGT
TTCTTTTCTC CATGGAAAAC TCCAAAGCCT ATAATGGACC GATGGGAGAA TCAAGGCAGT
CCTCAAACGA GTTTATCTGC TCCGGCCATA CCGCAGAACC TGCCCTTCCC ACCTGCCCTT
CACAGGAGCT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CACGGAAGCC TGATCCATAT
GAGCTCTACG AGAAATCTAG GGCCATTTAT GAAAAACAAG TGCCACCCAG GACCAGTTTT
CGAATGGATT CCTCTGGTCC AGATTATAAG GAATTGGATG TTCATCTTCG GAGGATGGAG
TCTGGATTTG GCTTCAGAAT CCTCGGGGGA GATGAGCCTG GACAGCCTAT TTTGATTGGA
GCTGTCATTG CCATGGGCTC AGCCGACAGA GATGGCCGCC TTCACCCAGG AGATGAGCTT
GTGTATGTTG ATGGGATTCC AGTAGCCGGC AAAACCCACC GCTATGTCAT CGACCTCATG
CACCACGCAG CCCGCAATGG GCAGGTCAAC CTCACTGTGA GAAGAAAGGT GCTATGTGGA
GGGGAGCCCT GCCCAGAGAA CGGGAGAAGT CCAGGCTCTG TATCCACCCA CCACAGCTCT
CCACGCAGTG ACTACGCAAC CTACACCAAC AGCAACCACG CTGCCCCCAG TAGCAATGCC
TCTCCCCCTG AAGGCTTCGC CTCCCACAGC CTGCAGACCA GTGATGTGGT CATTCACCGC
AAAGAGAATG AGGGCTTCGG CTTTGTCATC ATCAGCTCCC TGAACAGGCC TGAGTCTGGA
TCCACTATAA CTGTGCCCCA TAAAATCGGA CGCATCATTG ATGGGAGTCC TGCAGATCGC
TGTGCAAAAC TAAAAGTGGG AGACCGGATC CTAGCAGTGA ATGGCCAGTC TATCATCAAC
ATGCCTCACG CTGACATCGT GAAGCTCATC AAGGATGCAG GTCTTAGTGT CACCCTTCGC
ATCATTCCTC AGGAGGAGCT CAACAGCCCC ACCTCGGCAC CCAGCTCAGA GAAGCAGAGT
CCCATGGCGC AGCAGAGTCC CCTGGCACAG CAGAGTCCCC TGGCCCAGCC AAGCCCAGCC
ACCCCCAACA GCCCCATCGC CCAGCCAGCA CCACCTCAAC CACTTCAGCT GCAAGGACAC
GAAAATAGTT ACAGGTCGGA AGTGAAAGCA AGGCAAGATG TGAAACCAGA CATCCGACAG
CCTCCATTCA CAGACTACAG GCAGCCCCCG CTGGATTACA GGCAACCCCC AGGAGGGGAC
TACCAGCAGC CCCCACCCTT GGACTACAGG CAGCCTCCCC TACTGGACTA CAGGCAGCAC
TCCCCCGACA CCAGGCAGTA CCCTCTGTCG GACTACAGAC AACCCCAGGA TTTTGATTAT
TTCACTGTGG ACATGGAGAA AGGAGCCAAA GGATTTGGAT TCAGCATTCG TGGAGGAAGG
GAATACAAAA TGGATTTGTA TGTGTTGAGA CTGGCAGAAG ATGGACCAGC AATAAGGAAT
GGGAGGATGA GGGTAGGAGA TCAAATCATT GAAATCAATG GGGAAAGCAC AAGGGACATG
ACACATGCCA GAGCAATAGA ACTCATCAAA TCTGGAGGAA GACGAGTGAG GCTGCTGCTC
AAGAGAGGCA CGGGACAGGT CCCAGAATAT GGAATGGTAC CTTCCAGTCT CTCCATGTGC
ATGAAAAGTG ACAAGCATGG GTCCCCATAT TTCTACTTAT TGGGCCACCC TAAAGACACG
GAAAGATCCA AACTGGCTAG AACTGACAAT AGGAAGGACG AACCCGCCCC CTGGAGTTCT
CCCGCTGCCG CCGCCCCAGG TCTGCCGGAA GTAGGCGTCT CCCTGGACGA CGGCCTCGCT
CCATTCTCTC CATCACATCC AGCCCCACCC TCCGACCCTT CCCACCAGAT AAGCCCAGGC
CCAACTTGGG ATATCAAACG GGAACACGAC GTTAGGAAAC CAAAGGAGCT TTCAGCCTGC
GGCCAGAAGA AGCAGCGCCT CGGGGAGCAG AGGGAGCGCT CGGCGAGTCC GCAGAGGGCC
GCGCGGCCGA GGCTCGAGGA GGCGCCCGGC GGCCAGGGGC GGCCCGAGGC CGGCAGGCCC
GCCTCGGAGG CCAGGGCGCC CGGGCTCGCG GCGGCAGACG CGGCGGACGC GGCGCGGGCG
GGCGGGAAGG AGGCGCCCCG TGCGGCGGCG GGCTCCGAGC TCTGCCGGCG CGAAGGCCCG
GGGGCTGCGC CGGCGTTTGC CGGCCCGGGC GGCGGCGGCA GCGGCGCGCT GGAGGCCGAG
GGCAGGGCGG GTGCGCGCGC GGGTCCCCGG CCGGGCCCGC GACCCCCGGG GGGCGCCCCG
GCGCGCAAGG CCGCCGTCGC GCCGGGGCCC TGGAAGGTGC CGGGTTCTGA CAAGCTGCCC
AGCGTCCTCA AACCCGGCGC CTCGGCCGCC AGCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97643
Clone ID Related Accession (Same CDS sequence) XM_017012850.1
Accession Version XM_017012850.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGAATG
GTACCTTCCA GTCTCTCCAT GTGCATGAAA AGTGACAAGC ATGGGTCCCC ATATTTCTAC
TTATTGGGCC ACCCTAAAGA CACGACGAAC CCGCCCCCTG GAGTTCTCCC GCTGCCGCCG
CCCCAGGTCT GCCGGAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868339.1
CDS486..4466
Translation

Target ORF information:

RefSeq Version XM_017012850.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012850.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGAATG
GTACCTTCCA GTCTCTCCAT GTGCATGAAA AGTGACAAGC ATGGGTCCCC ATATTTCTAC
TTATTGGGCC ACCCTAAAGA CACGACGAAC CCGCCCCCTG GAGTTCTCCC GCTGCCGCCG
CCCCAGGTCT GCCGGAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97644
Clone ID Related Accession (Same CDS sequence) XM_017012851.1
Accession Version XM_017012851.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGCTGT
TCCTCCCCTT CCTTTAGGAA TGGTACCTTC CAGTCTCTCC ATGTGCATGA AAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868340.1
CDS486..4382
Translation

Target ORF information:

RefSeq Version XM_017012851.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012851.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGCTGT
TCCTCCCCTT CCTTTAGGAA TGGTACCTTC CAGTCTCTCC ATGTGCATGA AAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97645
Clone ID Related Accession (Same CDS sequence) XM_017012852.1
Accession Version XM_017012852.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGAAAG
ATCCAAACTG GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868341.1
CDS486..4340
Translation

Target ORF information:

RefSeq Version XM_017012852.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012852.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGAAAG
ATCCAAACTG GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97634
Clone ID Related Accession (Same CDS sequence) XM_017012840.1
Accession Version XM_017012840.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGAATG
GTACCTTCCA GTCTCTCCAT GTGCATGAAA AGTGACAAGC ATGGGTCCCC ATATTTCTAC
TTATTGGGCC ACCCTAAAGA CACGGAAAGA TCCAAACTGG CTAGAACTGA CAATAGGAAG
GACGAACCCG CCCCCTGGAG TTCTCCCGCT GCCGCCGCCC CAGGTCTGCC GGAAGTAGGC
GTCTCCCTGG ACGACGGCCT CGCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
CCTTCCCACC AGATAAGCCC AGGCCCAACT TGGGATATCA AACGGGAACA CGACGTTAGG
AAACCAAAGG AGCTTTCAGC CTGCGGCCAG AAGAAGCAGC GCCTCGGGGA GCAGAGGGAG
CGCTCGGCGA GTCCGCAGAG GGCCGCGCGG CCGAGGCTCG AGGAGGCGCC CGGCGGCCAG
GGGCGGCCCG AGGCCGGCAG GCCCGCCTCG GAGGCCAGGG CGCCCGGGCT CGCGGCGGCA
GACGCGGCGG ACGCGGCGCG GGCGGGCGGG AAGGAGGCGC CCCGTGCGGC GGCGGGCTCC
GAGCTCTGCC GGCGCGAAGG CCCGGGGGCT GCGCCGGCGT TTGCCGGCCC GGGCGGCGGC
GGCAGCGGCG CGCTGGAGGC CGAGGGCAGG GCGGGTGCGC GCGCGGGTCC CCGGCCGGGC
CCGCGACCCC CGGGGGGCGC CCCGGCGCGC AAGGCCGCCG TCGCGCCGGG GCCCTGGAAG
GTGCCGGGTT CTGACAAGCT GCCCAGCGTC CTCAAACCCG GCGCCTCGGC CGCCAGCAGA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868329.1
CDS486..5108
Translation

Target ORF information:

RefSeq Version XM_017012840.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012840.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCAA
CAAGTGCCAC CCAGGACCAG TTTTCGAATG GATTCCTCTG GTCCAGATTA TAAGGAATTG
GATGTTCATC TTCGGAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
CCTGGACAGC CTATTTTGAT TGGAGCTGTC ATTGCCATGG GCTCAGCCGA CAGAGATGGC
CGCCTTCACC CAGGAGATGA GCTTGTGTAT GTTGATGGGA TTCCAGTAGC CGGCAAAACC
CACCGCTATG TCATCGACCT CATGCACCAC GCAGCCCGCA ATGGGCAGGT CAACCTCACT
GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAACGGGAG AAGTCCAGGC
TCTGTATCCA CCCACCACAG CTCTCCACGC AGTGACTACG CAACCTACAC CAACAGCAAC
CACGCTGCCC CCAGTAGCAA TGCCTCTCCC CCTGAAGGCT TCGCCTCCCA CAGCCTGCAG
ACCAGTGATG TGGTCATTCA CCGCAAAGAG AATGAGGGCT TCGGCTTTGT CATCATCAGC
TCCCTGAACA GGCCTGAGTC TGGATCCACT ATAACTGTGC CCCATAAAAT CGGACGCATC
ATTGATGGGA GTCCTGCAGA TCGCTGTGCA AAACTAAAAG TGGGAGACCG GATCCTAGCA
GTGAATGGCC AGTCTATCAT CAACATGCCT CACGCTGACA TCGTGAAGCT CATCAAGGAT
GCAGGTCTTA GTGTCACCCT TCGCATCATT CCTCAGGAGG AGCTCAACAG CCCCACCTCG
GCACCCAGCT CAGAGAAGCA GAGTCCCATG GCGCAGCAGA GTCCCCTGGC ACAGCAGAGT
CCCCTGGCCC AGCCAAGCCC AGCCACCCCC AACAGCCCCA TCGCCCAGCC AGCACCACCT
CAACCACTTC AGCTGCAAGG ACACGAAAAT AGTTACAGGT CGGAAGTGAA AGCAAGGCAA
GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACAGACT ACAGGCAGCC CCCGCTGGAT
TACAGGCAAC CCCCAGGAGG GGACTACCAG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
CCCCTACTGG ACTACAGGCA GCACTCCCCC GACACCAGGC AGTACCCTCT GTCGGACTAC
AGACAACCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
GGATTCAGCA TTCGTGGAGG AAGGGAATAC AAAATGGATT TGTATGTGTT GAGACTGGCA
GAAGATGGAC CAGCAATAAG GAATGGGAGG ATGAGGGTAG GAGATCAAAT CATTGAAATC
AATGGGGAAA GCACAAGGGA CATGACACAT GCCAGAGCAA TAGAACTCAT CAAATCTGGA
GGAAGACGAG TGAGGCTGCT GCTCAAGAGA GGCACGGGAC AGGTCCCAGA ATATGGAATG
GTACCTTCCA GTCTCTCCAT GTGCATGAAA AGTGACAAGC ATGGGTCCCC ATATTTCTAC
TTATTGGGCC ACCCTAAAGA CACGGAAAGA TCCAAACTGG CTAGAACTGA CAATAGGAAG
GACGAACCCG CCCCCTGGAG TTCTCCCGCT GCCGCCGCCC CAGGTCTGCC GGAAGTAGGC
GTCTCCCTGG ACGACGGCCT CGCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
CCTTCCCACC AGATAAGCCC AGGCCCAACT TGGGATATCA AACGGGAACA CGACGTTAGG
AAACCAAAGG AGCTTTCAGC CTGCGGCCAG AAGAAGCAGC GCCTCGGGGA GCAGAGGGAG
CGCTCGGCGA GTCCGCAGAG GGCCGCGCGG CCGAGGCTCG AGGAGGCGCC CGGCGGCCAG
GGGCGGCCCG AGGCCGGCAG GCCCGCCTCG GAGGCCAGGG CGCCCGGGCT CGCGGCGGCA
GACGCGGCGG ACGCGGCGCG GGCGGGCGGG AAGGAGGCGC CCCGTGCGGC GGCGGGCTCC
GAGCTCTGCC GGCGCGAAGG CCCGGGGGCT GCGCCGGCGT TTGCCGGCCC GGGCGGCGGC
GGCAGCGGCG CGCTGGAGGC CGAGGGCAGG GCGGGTGCGC GCGCGGGTCC CCGGCCGGGC
CCGCGACCCC CGGGGGGCGC CCCGGCGCGC AAGGCCGCCG TCGCGCCGGG GCCCTGGAAG
GTGCCGGGTT CTGACAAGCT GCCCAGCGTC CTCAAACCCG GCGCCTCGGC CGCCAGCAGA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97635
Clone ID Related Accession (Same CDS sequence) XM_017012841.1
Accession Version XM_017012841.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGACAGC TCCTCATGGA CATTACCTAG GCCCAAATCC
ATCCGCCCCC GTGCTGCCCC GGAGAGGTCG CGCAGCGTGA ATGACTTGTC TTACTATCAG
CGCGACCCAG CAGGGAGGCC CTGGCTGTTC GAAAAACCAC TCTTCACCCG GGATGCATCC
CAGTTGAAGG GAACATTCCT CAGCACCACC CTAAAAAAGA GCAACATGGG CTTTGGATTT
ACCATCATTG GTGGAGACGA GCCTGATGAG TTTCTGCAGG TGAAAAGTGT GATTCCGGAT
GGGCCTGCAG CACAGGATGG AAAAATGGAA ACAGGTGATG TCATTGTCTA TATTAATGAA
GTTTGTGTCC TTGGACACAC TCATGCAGAT GTTGTCAAAC TTTTCCAGTC TGTTCCTATT
GGTCAGAGTG TCAACCTGGT GTTGTGTCGT GGCTACCCTT TGCCCTTTGA TCCTGAAGAC
CCTGCTAACA GCATGGTGCC ACCCCTTGCA ATAATGGAGA GGCCACCTCC AGTGATGGTC
AATGGAAGAC ACAACTATGA AACATATTTG GAGTACATTT CTCGGACCTC ACAGTCAGTT
CCAGATATAA CAGATCGGCC GCCTCATTCT CTGCACTCCA TGCCAACTGA TGGTCAGCTA
GACGGCACGT ATCCACCGCC CGTCCATGAT GACAATGTGT CTATGGCTTC ATCTGGGGCC
ACCCAAGCTG AACTTATGAC CTTAACCATT GTGAAAGGTG CCCAGGGCTT CGGCTTCACT
ATTGCCGACA GTCCTACAGG ACAGCGGGTG AAACAAATAC TTGACATTCA GGGATGCCCT
GGCCTGTGTG AAGGCGACCT CATTGTTGAG ATCAACCAGC AGAATGTACA GAACCTGAGC
CATACAGAAG TAGTGGATAT ACTTAAGGAC TGTCCCATTG GAAGTGAAAC TTCTTTGATT
ATCCATCGAG GAGGTTTCTT TTCTCCATGG AAAACTCCAA AGCCTATAAT GGACCGATGG
GAGAATCAAG GCAGTCCTCA AACGAGTTTA TCTGCTCCGG CCATACCGCA GAACCTGCCC
TTCCCACCTG CCCTTCACAG GAGCTCCTTT CCTGACTCAA CAGAGGCCTT TGACCCACGG
AAGCCTGATC CATATGAGCT CTACGAGAAA TCTAGGGCCA TTTATGAAAG TAGGCAACAA
GTGCCACCCA GGACCAGTTT TCGAATGGAT TCCTCTGGTC CAGATTATAA GGAATTGGAT
GTTCATCTTC GGAGGATGGA GTCTGGATTT GGCTTCAGAA TCCTCGGGGG AGATGAGCCT
GGACAGCCTA TTTTGATTGG AGCTGTCATT GCCATGGGCT CAGCCGACAG AGATGGCCGC
CTTCACCCAG GAGATGAGCT TGTGTATGTT GATGGGATTC CAGTAGCCGG CAAAACCCAC
CGCTATGTCA TCGACCTCAT GCACCACGCA GCCCGCAATG GGCAGGTCAA CCTCACTGTG
AGAAGAAAGG TGCTATGTGG AGGGGAGCCC TGCCCAGAGA ACGGGAGAAG TCCAGGCTCT
GTATCCACCC ACCACAGCTC TCCACGCAGT GACTACGCAA CCTACACCAA CAGCAACCAC
GCTGCCCCCA GTAGCAATGC CTCTCCCCCT GAAGGCTTCG CCTCCCACAG CCTGCAGACC
AGTGATGTGG TCATTCACCG CAAAGAGAAT GAGGGCTTCG GCTTTGTCAT CATCAGCTCC
CTGAACAGGC CTGAGTCTGG ATCCACTATA ACTGTGCCCC ATAAAATCGG ACGCATCATT
GATGGGAGTC CTGCAGATCG CTGTGCAAAA CTAAAAGTGG GAGACCGGAT CCTAGCAGTG
AATGGCCAGT CTATCATCAA CATGCCTCAC GCTGACATCG TGAAGCTCAT CAAGGATGCA
GGTCTTAGTG TCACCCTTCG CATCATTCCT CAGGAGGAGC TCAACAGCCC CACCTCGGCA
CCCAGCTCAG AGAAGCAGAG TCCCATGGCG CAGCAGAGTC CCCTGGCACA GCAGAGTCCC
CTGGCCCAGC CAAGCCCAGC CACCCCCAAC AGCCCCATCG CCCAGCCAGC ACCACCTCAA
CCACTTCAGC TGCAAGGACA CGAAAATAGT TACAGGTCGG AAGTGAAAGC AAGGCAAGAT
GTGAAACCAG ACATCCGACA GCCTCCATTC ACAGACTACA GGCAGCCCCC GCTGGATTAC
AGGCAACCCC CAGGAGGGGA CTACCAGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCC
CTACTGGACT ACAGGCAGCA CTCCCCCGAC ACCAGGCAGT ACCCTCTGTC GGACTACAGA
CAACCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
TTCAGCATTC GTGGAGGAAG GGAATACAAA ATGGATTTGT ATGTGTTGAG ACTGGCAGAA
GATGGACCAG CAATAAGGAA TGGGAGGATG AGGGTAGGAG ATCAAATCAT TGAAATCAAT
GGGGAAAGCA CAAGGGACAT GACACATGCC AGAGCAATAG AACTCATCAA ATCTGGAGGA
AGACGAGTGA GGCTGCTGCT CAAGAGAGGC ACGGGACAGG TCCCAGAATA TGGAATGGTA
CCTTCCAGTC TCTCCATGTG CATGAAAAGT GACAAGCATG GGTCCCCATA TTTCTACTTA
TTGGGCCACC CTAAAGACAC GGAAAGATCC AAACTGGCTA GAACTGACAA TAGGAAGGAC
GAACCCGCCC CCTGGAGTTC TCCCGCTGCC GCCGCCCCAG GTCTGCCGGA AGTAGGCGTC
TCCCTGGACG ACGGCCTCGC TCCATTCTCT CCATCACATC CAGCCCCACC CTCCGACCCT
TCCCACCAGA TAAGCCCAGG CCCAACTTGG GATATCAAAC GGGAACACGA CGTTAGGAAA
CCAAAGGAGC TTTCAGCCTG CGGCCAGAAG AAGCAGCGCC TCGGGGAGCA GAGGGAGCGC
TCGGCGAGTC CGCAGAGGGC CGCGCGGCCG AGGCTCGAGG AGGCGCCCGG CGGCCAGGGG
CGGCCCGAGG CCGGCAGGCC CGCCTCGGAG GCCAGGGCGC CCGGGCTCGC GGCGGCAGAC
GCGGCGGACG CGGCGCGGGC GGGCGGGAAG GAGGCGCCCC GTGCGGCGGC GGGCTCCGAG
CTCTGCCGGC GCGAAGGCCC GGGGGCTGCG CCGGCGTTTG CCGGCCCGGG CGGCGGCGGC
AGCGGCGCGC TGGAGGCCGA GGGCAGGGCG GGTGCGCGCG CGGGTCCCCG GCCGGGCCCG
CGACCCCCGG GGGGCGCCCC GGCGCGCAAG GCCGCCGTCG CGCCGGGGCC CTGGAAGGTG
CCGGGTTCTG ACAAGCTGCC CAGCGTCCTC AAACCCGGCG CCTCGGCCGC CAGCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868330.1
CDS486..5105
Translation

Target ORF information:

RefSeq Version XM_017012841.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012841.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGACAGC TCCTCATGGA CATTACCTAG GCCCAAATCC
ATCCGCCCCC GTGCTGCCCC GGAGAGGTCG CGCAGCGTGA ATGACTTGTC TTACTATCAG
CGCGACCCAG CAGGGAGGCC CTGGCTGTTC GAAAAACCAC TCTTCACCCG GGATGCATCC
CAGTTGAAGG GAACATTCCT CAGCACCACC CTAAAAAAGA GCAACATGGG CTTTGGATTT
ACCATCATTG GTGGAGACGA GCCTGATGAG TTTCTGCAGG TGAAAAGTGT GATTCCGGAT
GGGCCTGCAG CACAGGATGG AAAAATGGAA ACAGGTGATG TCATTGTCTA TATTAATGAA
GTTTGTGTCC TTGGACACAC TCATGCAGAT GTTGTCAAAC TTTTCCAGTC TGTTCCTATT
GGTCAGAGTG TCAACCTGGT GTTGTGTCGT GGCTACCCTT TGCCCTTTGA TCCTGAAGAC
CCTGCTAACA GCATGGTGCC ACCCCTTGCA ATAATGGAGA GGCCACCTCC AGTGATGGTC
AATGGAAGAC ACAACTATGA AACATATTTG GAGTACATTT CTCGGACCTC ACAGTCAGTT
CCAGATATAA CAGATCGGCC GCCTCATTCT CTGCACTCCA TGCCAACTGA TGGTCAGCTA
GACGGCACGT ATCCACCGCC CGTCCATGAT GACAATGTGT CTATGGCTTC ATCTGGGGCC
ACCCAAGCTG AACTTATGAC CTTAACCATT GTGAAAGGTG CCCAGGGCTT CGGCTTCACT
ATTGCCGACA GTCCTACAGG ACAGCGGGTG AAACAAATAC TTGACATTCA GGGATGCCCT
GGCCTGTGTG AAGGCGACCT CATTGTTGAG ATCAACCAGC AGAATGTACA GAACCTGAGC
CATACAGAAG TAGTGGATAT ACTTAAGGAC TGTCCCATTG GAAGTGAAAC TTCTTTGATT
ATCCATCGAG GAGGTTTCTT TTCTCCATGG AAAACTCCAA AGCCTATAAT GGACCGATGG
GAGAATCAAG GCAGTCCTCA AACGAGTTTA TCTGCTCCGG CCATACCGCA GAACCTGCCC
TTCCCACCTG CCCTTCACAG GAGCTCCTTT CCTGACTCAA CAGAGGCCTT TGACCCACGG
AAGCCTGATC CATATGAGCT CTACGAGAAA TCTAGGGCCA TTTATGAAAG TAGGCAACAA
GTGCCACCCA GGACCAGTTT TCGAATGGAT TCCTCTGGTC CAGATTATAA GGAATTGGAT
GTTCATCTTC GGAGGATGGA GTCTGGATTT GGCTTCAGAA TCCTCGGGGG AGATGAGCCT
GGACAGCCTA TTTTGATTGG AGCTGTCATT GCCATGGGCT CAGCCGACAG AGATGGCCGC
CTTCACCCAG GAGATGAGCT TGTGTATGTT GATGGGATTC CAGTAGCCGG CAAAACCCAC
CGCTATGTCA TCGACCTCAT GCACCACGCA GCCCGCAATG GGCAGGTCAA CCTCACTGTG
AGAAGAAAGG TGCTATGTGG AGGGGAGCCC TGCCCAGAGA ACGGGAGAAG TCCAGGCTCT
GTATCCACCC ACCACAGCTC TCCACGCAGT GACTACGCAA CCTACACCAA CAGCAACCAC
GCTGCCCCCA GTAGCAATGC CTCTCCCCCT GAAGGCTTCG CCTCCCACAG CCTGCAGACC
AGTGATGTGG TCATTCACCG CAAAGAGAAT GAGGGCTTCG GCTTTGTCAT CATCAGCTCC
CTGAACAGGC CTGAGTCTGG ATCCACTATA ACTGTGCCCC ATAAAATCGG ACGCATCATT
GATGGGAGTC CTGCAGATCG CTGTGCAAAA CTAAAAGTGG GAGACCGGAT CCTAGCAGTG
AATGGCCAGT CTATCATCAA CATGCCTCAC GCTGACATCG TGAAGCTCAT CAAGGATGCA
GGTCTTAGTG TCACCCTTCG CATCATTCCT CAGGAGGAGC TCAACAGCCC CACCTCGGCA
CCCAGCTCAG AGAAGCAGAG TCCCATGGCG CAGCAGAGTC CCCTGGCACA GCAGAGTCCC
CTGGCCCAGC CAAGCCCAGC CACCCCCAAC AGCCCCATCG CCCAGCCAGC ACCACCTCAA
CCACTTCAGC TGCAAGGACA CGAAAATAGT TACAGGTCGG AAGTGAAAGC AAGGCAAGAT
GTGAAACCAG ACATCCGACA GCCTCCATTC ACAGACTACA GGCAGCCCCC GCTGGATTAC
AGGCAACCCC CAGGAGGGGA CTACCAGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCC
CTACTGGACT ACAGGCAGCA CTCCCCCGAC ACCAGGCAGT ACCCTCTGTC GGACTACAGA
CAACCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
TTCAGCATTC GTGGAGGAAG GGAATACAAA ATGGATTTGT ATGTGTTGAG ACTGGCAGAA
GATGGACCAG CAATAAGGAA TGGGAGGATG AGGGTAGGAG ATCAAATCAT TGAAATCAAT
GGGGAAAGCA CAAGGGACAT GACACATGCC AGAGCAATAG AACTCATCAA ATCTGGAGGA
AGACGAGTGA GGCTGCTGCT CAAGAGAGGC ACGGGACAGG TCCCAGAATA TGGAATGGTA
CCTTCCAGTC TCTCCATGTG CATGAAAAGT GACAAGCATG GGTCCCCATA TTTCTACTTA
TTGGGCCACC CTAAAGACAC GGAAAGATCC AAACTGGCTA GAACTGACAA TAGGAAGGAC
GAACCCGCCC CCTGGAGTTC TCCCGCTGCC GCCGCCCCAG GTCTGCCGGA AGTAGGCGTC
TCCCTGGACG ACGGCCTCGC TCCATTCTCT CCATCACATC CAGCCCCACC CTCCGACCCT
TCCCACCAGA TAAGCCCAGG CCCAACTTGG GATATCAAAC GGGAACACGA CGTTAGGAAA
CCAAAGGAGC TTTCAGCCTG CGGCCAGAAG AAGCAGCGCC TCGGGGAGCA GAGGGAGCGC
TCGGCGAGTC CGCAGAGGGC CGCGCGGCCG AGGCTCGAGG AGGCGCCCGG CGGCCAGGGG
CGGCCCGAGG CCGGCAGGCC CGCCTCGGAG GCCAGGGCGC CCGGGCTCGC GGCGGCAGAC
GCGGCGGACG CGGCGCGGGC GGGCGGGAAG GAGGCGCCCC GTGCGGCGGC GGGCTCCGAG
CTCTGCCGGC GCGAAGGCCC GGGGGCTGCG CCGGCGTTTG CCGGCCCGGG CGGCGGCGGC
AGCGGCGCGC TGGAGGCCGA GGGCAGGGCG GGTGCGCGCG CGGGTCCCCG GCCGGGCCCG
CGACCCCCGG GGGGCGCCCC GGCGCGCAAG GCCGCCGTCG CGCCGGGGCC CTGGAAGGTG
CCGGGTTCTG ACAAGCTGCC CAGCGTCCTC AAACCCGGCG CCTCGGCCGC CAGCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97636
Clone ID Related Accession (Same CDS sequence) XM_017012842.1
Accession Version XM_017012842.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4617bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAAACAAGTG
CCACCCAGGA CCAGTTTTCG AATGGATTCC TCTGGTCCAG ATTATAAGGA ATTGGATGTT
CATCTTCGGA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
CAGCCTATTT TGATTGGAGC TGTCATTGCC ATGGGCTCAG CCGACAGAGA TGGCCGCCTT
CACCCAGGAG ATGAGCTTGT GTATGTTGAT GGGATTCCAG TAGCCGGCAA AACCCACCGC
TATGTCATCG ACCTCATGCA CCACGCAGCC CGCAATGGGC AGGTCAACCT CACTGTGAGA
AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAACG GGAGAAGTCC AGGCTCTGTA
TCCACCCACC ACAGCTCTCC ACGCAGTGAC TACGCAACCT ACACCAACAG CAACCACGCT
GCCCCCAGTA GCAATGCCTC TCCCCCTGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGT
GATGTGGTCA TTCACCGCAA AGAGAATGAG GGCTTCGGCT TTGTCATCAT CAGCTCCCTG
AACAGGCCTG AGTCTGGATC CACTATAACT GTGCCCCATA AAATCGGACG CATCATTGAT
GGGAGTCCTG CAGATCGCTG TGCAAAACTA AAAGTGGGAG ACCGGATCCT AGCAGTGAAT
GGCCAGTCTA TCATCAACAT GCCTCACGCT GACATCGTGA AGCTCATCAA GGATGCAGGT
CTTAGTGTCA CCCTTCGCAT CATTCCTCAG GAGGAGCTCA ACAGCCCCAC CTCGGCACCC
AGCTCAGAGA AGCAGAGTCC CATGGCGCAG CAGAGTCCCC TGGCACAGCA GAGTCCCCTG
GCCCAGCCAA GCCCAGCCAC CCCCAACAGC CCCATCGCCC AGCCAGCACC ACCTCAACCA
CTTCAGCTGC AAGGACACGA AAATAGTTAC AGGTCGGAAG TGAAAGCAAG GCAAGATGTG
AAACCAGACA TCCGACAGCC TCCATTCACA GACTACAGGC AGCCCCCGCT GGATTACAGG
CAACCCCCAG GAGGGGACTA CCAGCAGCCC CCACCCTTGG ACTACAGGCA GCCTCCCCTA
CTGGACTACA GGCAGCACTC CCCCGACACC AGGCAGTACC CTCTGTCGGA CTACAGACAA
CCCCAGGATT TTGATTATTT CACTGTGGAC ATGGAGAAAG GAGCCAAAGG ATTTGGATTC
AGCATTCGTG GAGGAAGGGA ATACAAAATG GATTTGTATG TGTTGAGACT GGCAGAAGAT
GGACCAGCAA TAAGGAATGG GAGGATGAGG GTAGGAGATC AAATCATTGA AATCAATGGG
GAAAGCACAA GGGACATGAC ACATGCCAGA GCAATAGAAC TCATCAAATC TGGAGGAAGA
CGAGTGAGGC TGCTGCTCAA GAGAGGCACG GGACAGGTCC CAGAATATGG AATGGTACCT
TCCAGTCTCT CCATGTGCAT GAAAAGTGAC AAGCATGGGT CCCCATATTT CTACTTATTG
GGCCACCCTA AAGACACGGA AAGATCCAAA CTGGCTAGAA CTGACAATAG GAAGGACGAA
CCCGCCCCCT GGAGTTCTCC CGCTGCCGCC GCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
CTGGACGACG GCCTCGCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
CACCAGATAA GCCCAGGCCC AACTTGGGAT ATCAAACGGG AACACGACGT TAGGAAACCA
AAGGAGCTTT CAGCCTGCGG CCAGAAGAAG CAGCGCCTCG GGGAGCAGAG GGAGCGCTCG
GCGAGTCCGC AGAGGGCCGC GCGGCCGAGG CTCGAGGAGG CGCCCGGCGG CCAGGGGCGG
CCCGAGGCCG GCAGGCCCGC CTCGGAGGCC AGGGCGCCCG GGCTCGCGGC GGCAGACGCG
GCGGACGCGG CGCGGGCGGG CGGGAAGGAG GCGCCCCGTG CGGCGGCGGG CTCCGAGCTC
TGCCGGCGCG AAGGCCCGGG GGCTGCGCCG GCGTTTGCCG GCCCGGGCGG CGGCGGCAGC
GGCGCGCTGG AGGCCGAGGG CAGGGCGGGT GCGCGCGCGG GTCCCCGGCC GGGCCCGCGA
CCCCCGGGGG GCGCCCCGGC GCGCAAGGCC GCCGTCGCGC CGGGGCCCTG GAAGGTGCCG
GGTTCTGACA AGCTGCCCAG CGTCCTCAAA CCCGGCGCCT CGGCCGCCAG CAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868331.1
CDS486..5102
Translation

Target ORF information:

RefSeq Version XM_017012842.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012842.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAAACAAGTG
CCACCCAGGA CCAGTTTTCG AATGGATTCC TCTGGTCCAG ATTATAAGGA ATTGGATGTT
CATCTTCGGA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
CAGCCTATTT TGATTGGAGC TGTCATTGCC ATGGGCTCAG CCGACAGAGA TGGCCGCCTT
CACCCAGGAG ATGAGCTTGT GTATGTTGAT GGGATTCCAG TAGCCGGCAA AACCCACCGC
TATGTCATCG ACCTCATGCA CCACGCAGCC CGCAATGGGC AGGTCAACCT CACTGTGAGA
AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAACG GGAGAAGTCC AGGCTCTGTA
TCCACCCACC ACAGCTCTCC ACGCAGTGAC TACGCAACCT ACACCAACAG CAACCACGCT
GCCCCCAGTA GCAATGCCTC TCCCCCTGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGT
GATGTGGTCA TTCACCGCAA AGAGAATGAG GGCTTCGGCT TTGTCATCAT CAGCTCCCTG
AACAGGCCTG AGTCTGGATC CACTATAACT GTGCCCCATA AAATCGGACG CATCATTGAT
GGGAGTCCTG CAGATCGCTG TGCAAAACTA AAAGTGGGAG ACCGGATCCT AGCAGTGAAT
GGCCAGTCTA TCATCAACAT GCCTCACGCT GACATCGTGA AGCTCATCAA GGATGCAGGT
CTTAGTGTCA CCCTTCGCAT CATTCCTCAG GAGGAGCTCA ACAGCCCCAC CTCGGCACCC
AGCTCAGAGA AGCAGAGTCC CATGGCGCAG CAGAGTCCCC TGGCACAGCA GAGTCCCCTG
GCCCAGCCAA GCCCAGCCAC CCCCAACAGC CCCATCGCCC AGCCAGCACC ACCTCAACCA
CTTCAGCTGC AAGGACACGA AAATAGTTAC AGGTCGGAAG TGAAAGCAAG GCAAGATGTG
AAACCAGACA TCCGACAGCC TCCATTCACA GACTACAGGC AGCCCCCGCT GGATTACAGG
CAACCCCCAG GAGGGGACTA CCAGCAGCCC CCACCCTTGG ACTACAGGCA GCCTCCCCTA
CTGGACTACA GGCAGCACTC CCCCGACACC AGGCAGTACC CTCTGTCGGA CTACAGACAA
CCCCAGGATT TTGATTATTT CACTGTGGAC ATGGAGAAAG GAGCCAAAGG ATTTGGATTC
AGCATTCGTG GAGGAAGGGA ATACAAAATG GATTTGTATG TGTTGAGACT GGCAGAAGAT
GGACCAGCAA TAAGGAATGG GAGGATGAGG GTAGGAGATC AAATCATTGA AATCAATGGG
GAAAGCACAA GGGACATGAC ACATGCCAGA GCAATAGAAC TCATCAAATC TGGAGGAAGA
CGAGTGAGGC TGCTGCTCAA GAGAGGCACG GGACAGGTCC CAGAATATGG AATGGTACCT
TCCAGTCTCT CCATGTGCAT GAAAAGTGAC AAGCATGGGT CCCCATATTT CTACTTATTG
GGCCACCCTA AAGACACGGA AAGATCCAAA CTGGCTAGAA CTGACAATAG GAAGGACGAA
CCCGCCCCCT GGAGTTCTCC CGCTGCCGCC GCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
CTGGACGACG GCCTCGCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
CACCAGATAA GCCCAGGCCC AACTTGGGAT ATCAAACGGG AACACGACGT TAGGAAACCA
AAGGAGCTTT CAGCCTGCGG CCAGAAGAAG CAGCGCCTCG GGGAGCAGAG GGAGCGCTCG
GCGAGTCCGC AGAGGGCCGC GCGGCCGAGG CTCGAGGAGG CGCCCGGCGG CCAGGGGCGG
CCCGAGGCCG GCAGGCCCGC CTCGGAGGCC AGGGCGCCCG GGCTCGCGGC GGCAGACGCG
GCGGACGCGG CGCGGGCGGG CGGGAAGGAG GCGCCCCGTG CGGCGGCGGG CTCCGAGCTC
TGCCGGCGCG AAGGCCCGGG GGCTGCGCCG GCGTTTGCCG GCCCGGGCGG CGGCGGCAGC
GGCGCGCTGG AGGCCGAGGG CAGGGCGGGT GCGCGCGCGG GTCCCCGGCC GGGCCCGCGA
CCCCCGGGGG GCGCCCCGGC GCGCAAGGCC GCCGTCGCGC CGGGGCCCTG GAAGGTGCCG
GGTTCTGACA AGCTGCCCAG CGTCCTCAAA CCCGGCGCCT CGGCCGCCAG CAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97637
Clone ID Related Accession (Same CDS sequence) XM_017012843.1
Accession Version XM_017012843.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4581bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCGT
CCAGATTATA AGGAATTGGA TGTTCATCTT CGGAGGATGG AGTCTGGATT TGGCTTCAGA
ATCCTCGGGG GAGATGAGCC TGGACAGCCT ATTTTGATTG GAGCTGTCAT TGCCATGGGC
TCAGCCGACA GAGATGGCCG CCTTCACCCA GGAGATGAGC TTGTGTATGT TGATGGGATT
CCAGTAGCCG GCAAAACCCA CCGCTATGTC ATCGACCTCA TGCACCACGC AGCCCGCAAT
GGGCAGGTCA ACCTCACTGT GAGAAGAAAG GTGCTATGTG GAGGGGAGCC CTGCCCAGAG
AACGGGAGAA GTCCAGGCTC TGTATCCACC CACCACAGCT CTCCACGCAG TGACTACGCA
ACCTACACCA ACAGCAACCA CGCTGCCCCC AGTAGCAATG CCTCTCCCCC TGAAGGCTTC
GCCTCCCACA GCCTGCAGAC CAGTGATGTG GTCATTCACC GCAAAGAGAA TGAGGGCTTC
GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCTGAGTCTG GATCCACTAT AACTGTGCCC
CATAAAATCG GACGCATCAT TGATGGGAGT CCTGCAGATC GCTGTGCAAA ACTAAAAGTG
GGAGACCGGA TCCTAGCAGT GAATGGCCAG TCTATCATCA ACATGCCTCA CGCTGACATC
GTGAAGCTCA TCAAGGATGC AGGTCTTAGT GTCACCCTTC GCATCATTCC TCAGGAGGAG
CTCAACAGCC CCACCTCGGC ACCCAGCTCA GAGAAGCAGA GTCCCATGGC GCAGCAGAGT
CCCCTGGCAC AGCAGAGTCC CCTGGCCCAG CCAAGCCCAG CCACCCCCAA CAGCCCCATC
GCCCAGCCAG CACCACCTCA ACCACTTCAG CTGCAAGGAC ACGAAAATAG TTACAGGTCG
GAAGTGAAAG CAAGGCAAGA TGTGAAACCA GACATCCGAC AGCCTCCATT CACAGACTAC
AGGCAGCCCC CGCTGGATTA CAGGCAACCC CCAGGAGGGG ACTACCAGCA GCCCCCACCC
TTGGACTACA GGCAGCCTCC CCTACTGGAC TACAGGCAGC ACTCCCCCGA CACCAGGCAG
TACCCTCTGT CGGACTACAG ACAACCCCAG GATTTTGATT ATTTCACTGT GGACATGGAG
AAAGGAGCCA AAGGATTTGG ATTCAGCATT CGTGGAGGAA GGGAATACAA AATGGATTTG
TATGTGTTGA GACTGGCAGA AGATGGACCA GCAATAAGGA ATGGGAGGAT GAGGGTAGGA
GATCAAATCA TTGAAATCAA TGGGGAAAGC ACAAGGGACA TGACACATGC CAGAGCAATA
GAACTCATCA AATCTGGAGG AAGACGAGTG AGGCTGCTGC TCAAGAGAGG CACGGGACAG
GTCCCAGAAT ATGGAATGGT ACCTTCCAGT CTCTCCATGT GCATGAAAAG TGACAAGCAT
GGGTCCCCAT ATTTCTACTT ATTGGGCCAC CCTAAAGACA CGGAAAGATC CAAACTGGCT
AGAACTGACA ATAGGAAGGA CGAACCCGCC CCCTGGAGTT CTCCCGCTGC CGCCGCCCCA
GGTCTGCCGG AAGTAGGCGT CTCCCTGGAC GACGGCCTCG CTCCATTCTC TCCATCACAT
CCAGCCCCAC CCTCCGACCC TTCCCACCAG ATAAGCCCAG GCCCAACTTG GGATATCAAA
CGGGAACACG ACGTTAGGAA ACCAAAGGAG CTTTCAGCCT GCGGCCAGAA GAAGCAGCGC
CTCGGGGAGC AGAGGGAGCG CTCGGCGAGT CCGCAGAGGG CCGCGCGGCC GAGGCTCGAG
GAGGCGCCCG GCGGCCAGGG GCGGCCCGAG GCCGGCAGGC CCGCCTCGGA GGCCAGGGCG
CCCGGGCTCG CGGCGGCAGA CGCGGCGGAC GCGGCGCGGG CGGGCGGGAA GGAGGCGCCC
CGTGCGGCGG CGGGCTCCGA GCTCTGCCGG CGCGAAGGCC CGGGGGCTGC GCCGGCGTTT
GCCGGCCCGG GCGGCGGCGG CAGCGGCGCG CTGGAGGCCG AGGGCAGGGC GGGTGCGCGC
GCGGGTCCCC GGCCGGGCCC GCGACCCCCG GGGGGCGCCC CGGCGCGCAA GGCCGCCGTC
GCGCCGGGGC CCTGGAAGGT GCCGGGTTCT GACAAGCTGC CCAGCGTCCT CAAACCCGGC
GCCTCGGCCG CCAGCAGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868332.1
CDS486..5066
Translation

Target ORF information:

RefSeq Version XM_017012843.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012843.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGTAGAC AGCTCCTCAT GGACATTACC TAGGCCCAAA
TCCATCCGCC CCCGTGCTGC CCCGGAGAGG TCGCGCAGCG TGAATGACTT GTCTTACTAT
CAGCGCGACC CAGCAGGGAG GCCCTGGCTG TTCGAAAAAC CACTCTTCAC CCGGGATGCA
TCCCAGTTGA AGGGAACATT CCTCAGCACC ACCCTAAAAA AGAGCAACAT GGGCTTTGGA
TTTACCATCA TTGGTGGAGA CGAGCCTGAT GAGTTTCTGC AGGTGAAAAG TGTGATTCCG
GATGGGCCTG CAGCACAGGA TGGAAAAATG GAAACAGGTG ATGTCATTGT CTATATTAAT
GAAGTTTGTG TCCTTGGACA CACTCATGCA GATGTTGTCA AACTTTTCCA GTCTGTTCCT
ATTGGTCAGA GTGTCAACCT GGTGTTGTGT CGTGGCTACC CTTTGCCCTT TGATCCTGAA
GACCCTGCTA ACAGCATGGT GCCACCCCTT GCAATAATGG AGAGGCCACC TCCAGTGATG
GTCAATGGAA GACACAACTA TGAAACATAT TTGGAGTACA TTTCTCGGAC CTCACAGTCA
GTTCCAGATA TAACAGATCG GCCGCCTCAT TCTCTGCACT CCATGCCAAC TGATGGTCAG
CTAGACGGCA CGTATCCACC GCCCGTCCAT GATGACAATG TGTCTATGGC TTCATCTGGG
GCCACCCAAG CTGAACTTAT GACCTTAACC ATTGTGAAAG GTGCCCAGGG CTTCGGCTTC
ACTATTGCCG ACAGTCCTAC AGGACAGCGG GTGAAACAAA TACTTGACAT TCAGGGATGC
CCTGGCCTGT GTGAAGGCGA CCTCATTGTT GAGATCAACC AGCAGAATGT ACAGAACCTG
AGCCATACAG AAGTAGTGGA TATACTTAAG GACTGTCCCA TTGGAAGTGA AACTTCTTTG
ATTATCCATC GAGGAGGTTT CTTTTCTCCA TGGAAAACTC CAAAGCCTAT AATGGACCGA
TGGGAGAATC AAGGCAGTCC TCAAACGAGT TTATCTGCTC CGGCCATACC GCAGAACCTG
CCCTTCCCAC CTGCCCTTCA CAGGAGCTCC TTTCCTGACT CAACAGAGGC CTTTGACCCA
CGGAAGCCTG ATCCATATGA GCTCTACGAG AAATCTAGGG CCATTTATGA AAGTAGGCGT
CCAGATTATA AGGAATTGGA TGTTCATCTT CGGAGGATGG AGTCTGGATT TGGCTTCAGA
ATCCTCGGGG GAGATGAGCC TGGACAGCCT ATTTTGATTG GAGCTGTCAT TGCCATGGGC
TCAGCCGACA GAGATGGCCG CCTTCACCCA GGAGATGAGC TTGTGTATGT TGATGGGATT
CCAGTAGCCG GCAAAACCCA CCGCTATGTC ATCGACCTCA TGCACCACGC AGCCCGCAAT
GGGCAGGTCA ACCTCACTGT GAGAAGAAAG GTGCTATGTG GAGGGGAGCC CTGCCCAGAG
AACGGGAGAA GTCCAGGCTC TGTATCCACC CACCACAGCT CTCCACGCAG TGACTACGCA
ACCTACACCA ACAGCAACCA CGCTGCCCCC AGTAGCAATG CCTCTCCCCC TGAAGGCTTC
GCCTCCCACA GCCTGCAGAC CAGTGATGTG GTCATTCACC GCAAAGAGAA TGAGGGCTTC
GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCTGAGTCTG GATCCACTAT AACTGTGCCC
CATAAAATCG GACGCATCAT TGATGGGAGT CCTGCAGATC GCTGTGCAAA ACTAAAAGTG
GGAGACCGGA TCCTAGCAGT GAATGGCCAG TCTATCATCA ACATGCCTCA CGCTGACATC
GTGAAGCTCA TCAAGGATGC AGGTCTTAGT GTCACCCTTC GCATCATTCC TCAGGAGGAG
CTCAACAGCC CCACCTCGGC ACCCAGCTCA GAGAAGCAGA GTCCCATGGC GCAGCAGAGT
CCCCTGGCAC AGCAGAGTCC CCTGGCCCAG CCAAGCCCAG CCACCCCCAA CAGCCCCATC
GCCCAGCCAG CACCACCTCA ACCACTTCAG CTGCAAGGAC ACGAAAATAG TTACAGGTCG
GAAGTGAAAG CAAGGCAAGA TGTGAAACCA GACATCCGAC AGCCTCCATT CACAGACTAC
AGGCAGCCCC CGCTGGATTA CAGGCAACCC CCAGGAGGGG ACTACCAGCA GCCCCCACCC
TTGGACTACA GGCAGCCTCC CCTACTGGAC TACAGGCAGC ACTCCCCCGA CACCAGGCAG
TACCCTCTGT CGGACTACAG ACAACCCCAG GATTTTGATT ATTTCACTGT GGACATGGAG
AAAGGAGCCA AAGGATTTGG ATTCAGCATT CGTGGAGGAA GGGAATACAA AATGGATTTG
TATGTGTTGA GACTGGCAGA AGATGGACCA GCAATAAGGA ATGGGAGGAT GAGGGTAGGA
GATCAAATCA TTGAAATCAA TGGGGAAAGC ACAAGGGACA TGACACATGC CAGAGCAATA
GAACTCATCA AATCTGGAGG AAGACGAGTG AGGCTGCTGC TCAAGAGAGG CACGGGACAG
GTCCCAGAAT ATGGAATGGT ACCTTCCAGT CTCTCCATGT GCATGAAAAG TGACAAGCAT
GGGTCCCCAT ATTTCTACTT ATTGGGCCAC CCTAAAGACA CGGAAAGATC CAAACTGGCT
AGAACTGACA ATAGGAAGGA CGAACCCGCC CCCTGGAGTT CTCCCGCTGC CGCCGCCCCA
GGTCTGCCGG AAGTAGGCGT CTCCCTGGAC GACGGCCTCG CTCCATTCTC TCCATCACAT
CCAGCCCCAC CCTCCGACCC TTCCCACCAG ATAAGCCCAG GCCCAACTTG GGATATCAAA
CGGGAACACG ACGTTAGGAA ACCAAAGGAG CTTTCAGCCT GCGGCCAGAA GAAGCAGCGC
CTCGGGGAGC AGAGGGAGCG CTCGGCGAGT CCGCAGAGGG CCGCGCGGCC GAGGCTCGAG
GAGGCGCCCG GCGGCCAGGG GCGGCCCGAG GCCGGCAGGC CCGCCTCGGA GGCCAGGGCG
CCCGGGCTCG CGGCGGCAGA CGCGGCGGAC GCGGCGCGGG CGGGCGGGAA GGAGGCGCCC
CGTGCGGCGG CGGGCTCCGA GCTCTGCCGG CGCGAAGGCC CGGGGGCTGC GCCGGCGTTT
GCCGGCCCGG GCGGCGGCGG CAGCGGCGCG CTGGAGGCCG AGGGCAGGGC GGGTGCGCGC
GCGGGTCCCC GGCCGGGCCC GCGACCCCCG GGGGGCGCCC CGGCGCGCAA GGCCGCCGTC
GCGCCGGGGC CCTGGAAGGT GCCGGGTTCT GACAAGCTGC CCAGCGTCCT CAAACCCGGC
GCCTCGGCCG CCAGCAGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18616
Clone ID Related Accession (Same CDS sequence) NM_012301.3
Accession Version NM_012301.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF038563.1, AC007237.3, AB014605.2, AC004808.1, AA448362.1 and AC004990.1. This sequence is a reference standard in the RefSeqGene project. On May 19, 2005 this sequence version replaced NM_012301.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF038563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGACGA ACCCGCCCCC
TGGAGTTCTC CCGCTGCCGC CGCCCCAGGT CTGCCGGAAG TAGGCGTCTC CCTGGACGAC
GGCCTCGCTC CATTCTCTCC ATCACATCCA GCCCCACCCT CCGACCCTTC CCACCAGATA
AGCCCAGGCC CAACTTGGGA TATCAAACGG GAACACGACG TTAGGAAACC AAAGGAGCTT
TCAGCCTGCG GCCAGAAGAA GCAGCGCCTC GGGGAGCAGA GGGAGCGCTC GGCGAGTCCG
CAGAGGGCCG CGCGGCCGAG GCTCGAGGAG GCGCCCGGCG GCCAGGGGCG GCCCGAGGCC
GGCAGGCCCG CCTCGGAGGC CAGGGCGCCC GGGCTCGCGG CGGCAGACGC GGCGGACGCG
GCGCGGGCGG GCGGGAAGGA GGCGCCCCGT GCGGCGGCGG GCTCCGAGCT CTGCCGGCGC
GAAGGCCCGG GGGCTGCGCC GGCGTTTGCC GGCCCGGGCG GCGGCGGCAG CGGCGCGCTG
GAGGCCGAGG GCAGGGCGGG TGCGCGCGCG GGTCCCCGGC CGGGCCCGCG ACCCCCGGGG
GGCGCCCCGG CGCGCAAGGC CGCCGTCGCG CCGGGGCCCT GGAAGGTGCC GGGTTCTGAC
AAGCTGCCCA GCGTCCTCAA ACCCGGCGCC TCGGCCGCCA GCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036433.2
CDS255..4622
Misc Feature(1)126..128
Misc Feature(2)318..542
Misc Feature(3)order(333..344,348..350,498..503,510..515)
Misc Feature(4)606..1127
Misc Feature(5)1158..1397
Misc Feature(6)1167..1259
Misc Feature(7)order(1209..1211,1242..1244)
Misc Feature(8)1302..1391
Misc Feature(9)1338..1340
Misc Feature(10)order(1347..1349,1380..1382)
Misc Feature(11)1536..1781
Misc Feature(12)order(1557..1568,1572..1574,1719..1724,1731..1736)
Misc Feature(13)2058..2303
Misc Feature(14)order(2094..2105,2109..2111,2238..2243,2250..2255)
Misc Feature(15)2298..2522
Misc Feature(16)2310..2312
Misc Feature(17)2577..2837
Misc Feature(18)order(2613..2624,2628..2630,2775..2780,2787..2792)
Misc Feature(19)2733..2735
Misc Feature(20)2904..2906
Misc Feature(21)2907..2909
Misc Feature(22)3012..3275
Misc Feature(23)order(3042..3053,3057..3059,3225..3230,3237..3242)
Misc Feature(24)3294..3296
Misc Feature(25)3690..3932
Misc Feature(26)order(3720..3731,3735..3737,3882..3887,3894..3899)
Exon (1)1..555
Exon (2)556..672
Exon (3)673..792
Exon (4)793..1008
Exon (5)1009..1219
Exon (6)1220..1299
Exon (7)1300..1357
Exon (8)1358..1479
Exon (9)1480..1662
Exon (10)1663..2301
Exon (11)2302..2333
Exon (12)2334..2523
Exon (13)2524..2565
Exon (14)2566..2657
Exon (15)2658..2850
Exon (16)2851..3099
Exon (17)3100..3285
Exon (18)3286..3457
Exon (19)3458..3677
Exon (20)3678..3821
Exon (21)3822..3960
Exon (22)3961..6880
Translation

Target ORF information:

RefSeq Version NM_012301.3
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_012301.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGACGA ACCCGCCCCC
TGGAGTTCTC CCGCTGCCGC CGCCCCAGGT CTGCCGGAAG TAGGCGTCTC CCTGGACGAC
GGCCTCGCTC CATTCTCTCC ATCACATCCA GCCCCACCCT CCGACCCTTC CCACCAGATA
AGCCCAGGCC CAACTTGGGA TATCAAACGG GAACACGACG TTAGGAAACC AAAGGAGCTT
TCAGCCTGCG GCCAGAAGAA GCAGCGCCTC GGGGAGCAGA GGGAGCGCTC GGCGAGTCCG
CAGAGGGCCG CGCGGCCGAG GCTCGAGGAG GCGCCCGGCG GCCAGGGGCG GCCCGAGGCC
GGCAGGCCCG CCTCGGAGGC CAGGGCGCCC GGGCTCGCGG CGGCAGACGC GGCGGACGCG
GCGCGGGCGG GCGGGAAGGA GGCGCCCCGT GCGGCGGCGG GCTCCGAGCT CTGCCGGCGC
GAAGGCCCGG GGGCTGCGCC GGCGTTTGCC GGCCCGGGCG GCGGCGGCAG CGGCGCGCTG
GAGGCCGAGG GCAGGGCGGG TGCGCGCGCG GGTCCCCGGC CGGGCCCGCG ACCCCCGGGG
GGCGCCCCGG CGCGCAAGGC CGCCGTCGCG CCGGGGCCCT GGAAGGTGCC GGGTTCTGAC
AAGCTGCCCA GCGTCCTCAA ACCCGGCGCC TCGGCCGCCA GCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41888
Clone ID Related Accession (Same CDS sequence) NM_001301128.1
Accession Version NM_001301128.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-08
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF038563.1, AB014605.2, AK297353.1, AC004808.1, AA448362.1, Z19922.1 and AA890379.1. On Jul 23, 2014 this sequence version replaced XM_005250725.2. Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150277.1, AB014605.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCG TCCAGATTAT
AAGGAATTGG ATGTTCATCT TCGGAGGATG GAGTCTGGAT TTGGCTTCAG AATCCTCGGG
GGAGATGAGC CTGGACAGCC TATTTTGATT GGAGCTGTCA TTGCCATGGG CTCAGCCGAC
AGAGATGGCC GCCTTCACCC AGGAGATGAG CTTGTGTATG TTGATGGGAT TCCAGTAGCC
GGCAAAACCC ACCGCTATGT CATCGACCTC ATGCACCACG CAGCCCGCAA TGGGCAGGTC
AACCTCACTG TGAGAAGAAA GGTGCTATGT GGAGGGGAGC CCTGCCCAGA GAACGGGAGA
AGTCCAGGCT CTGTATCCAC CCACCACAGC TCTCCACGCA GTGACTACGC AACCTACACC
AACAGCAACC ACGCTGCCCC CAGTAGCAAT GCCTCTCCCC CTGAAGGCTT CGCCTCCCAC
AGCCTGCAGA CCAGTGATGT GGTCATTCAC CGCAAAGAGA ATGAGGGCTT CGGCTTTGTC
ATCATCAGCT CCCTGAACAG GCCTGAGTCT GGATCCACTA TAACTGTGCC CCATAAAATC
GGACGCATCA TTGATGGGAG TCCTGCAGAT CGCTGTGCAA AACTAAAAGT GGGAGACCGG
ATCCTAGCAG TGAATGGCCA GTCTATCATC AACATGCCTC ACGCTGACAT CGTGAAGCTC
ATCAAGGATG CAGGTCTTAG TGTCACCCTT CGCATCATTC CTCAGGAGGA GCTCAACAGC
CCCACCTCGG CACCCAGCTC AGAGAAGCAG AGTCCCATGG CGCAGCAGAG TCCCCTGGCA
CAGCAGAGTC CCCTGGCCCA GCCAAGCCCA GCCACCCCCA ACAGCCCCAT CGCCCAGCCA
GCACCACCTC AACCACTTCA GCTGCAAGGA CACGAAAATA GTTACAGGTC GGAAGTGAAA
GCAAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACAGACTA CAGGCAGCCC
CCGCTGGATT ACAGGCAACC CCCAGGAGGG GACTACCAGC AGCCCCCACC CTTGGACTAC
AGGCAGCCTC CCCTACTGGA CTACAGGCAG CACTCCCCCG ACACCAGGCA GTACCCTCTG
TCGGACTACA GACAACCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAATACA AAATGGATTT GTATGTGTTG
AGACTGGCAG AAGATGGACC AGCAATAAGG AATGGGAGGA TGAGGGTAGG AGATCAAATC
ATTGAAATCA ATGGGGAAAG CACAAGGGAC ATGACACATG CCAGAGCAAT AGAACTCATC
AAATCTGGAG GAAGACGAGT GAGGCTGCTG CTCAAGAGAG GCACGGGACA GGTCCCAGAA
TATGACGAAC CCGCCCCCTG GAGTTCTCCC GCTGCCGCCG CCCCAGGTCT GCCGGAAGTA
GGCGTCTCCC TGGACGACGG CCTCGCTCCA TTCTCTCCAT CACATCCAGC CCCACCCTCC
GACCCTTCCC ACCAGATAAG CCCAGGCCCA ACTTGGGATA TCAAACGGGA ACACGACGTT
AGGAAACCAA AGGAGCTTTC AGCCTGCGGC CAGAAGAAGC AGCGCCTCGG GGAGCAGAGG
GAGCGCTCGG CGAGTCCGCA GAGGGCCGCG CGGCCGAGGC TCGAGGAGGC GCCCGGCGGC
CAGGGGCGGC CCGAGGCCGG CAGGCCCGCC TCGGAGGCCA GGGCGCCCGG GCTCGCGGCG
GCAGACGCGG CGGACGCGGC GCGGGCGGGC GGGAAGGAGG CGCCCCGTGC GGCGGCGGGC
TCCGAGCTCT GCCGGCGCGA AGGCCCGGGG GCTGCGCCGG CGTTTGCCGG CCCGGGCGGC
GGCGGCAGCG GCGCGCTGGA GGCCGAGGGC AGGGCGGGTG CGCGCGCGGG TCCCCGGCCG
GGCCCGCGAC CCCCGGGGGG CGCCCCGGCG CGCAAGGCCG CCGTCGCGCC GGGGCCCTGG
AAGGTGCCGG GTTCTGACAA GCTGCCCAGC GTCCTCAAAC CCGGCGCCTC GGCCGCCAGC
AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001288057.1
CDS255..4580
Misc Feature(1)126..128
Misc Feature(2)318..542
Misc Feature(3)order(333..344,348..350,498..503,510..515)
Misc Feature(4)606..1127
Misc Feature(5)1158..1397
Misc Feature(6)1167..1259
Misc Feature(7)order(1209..1211,1242..1244)
Misc Feature(8)1302..1391
Misc Feature(9)1338..1340
Misc Feature(10)order(1347..1349,1380..1382)
Misc Feature(11)1536..1781
Misc Feature(12)order(1557..1568,1572..1574,1719..1724,1731..1736)
Misc Feature(13)2058..2303
Misc Feature(14)order(2094..2105,2109..2111,2238..2243,2250..2255)
Misc Feature(15)2298..2522
Misc Feature(16)2310..2312
Misc Feature(17)2535..2795
Misc Feature(18)order(2571..2582,2586..2588,2733..2738,2745..2750)
Misc Feature(19)2691..2693
Misc Feature(20)2862..2864
Misc Feature(21)2865..2867
Misc Feature(22)2970..3233
Misc Feature(23)order(3000..3011,3015..3017,3183..3188,3195..3200)
Misc Feature(24)3252..3254
Misc Feature(25)3648..3890
Misc Feature(26)order(3678..3689,3693..3695,3840..3845,3852..3857)
Exon (1)1..555
Exon (2)556..672
Exon (3)673..792
Exon (4)793..1008
Exon (5)1009..1219
Exon (6)1220..1299
Exon (7)1300..1357
Exon (8)1358..1479
Exon (9)1480..1662
Exon (10)1663..2301
Exon (11)2302..2333
Exon (12)2334..2523
Exon (13)2524..2615
Exon (14)2616..2808
Exon (15)2809..3057
Exon (16)3058..3243
Exon (17)3244..3415
Exon (18)3416..3635
Exon (19)3636..3779
Exon (20)3780..3918
Exon (21)3919..6838
Translation

Target ORF information:

RefSeq Version NM_001301128.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001301128.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCG TCCAGATTAT
AAGGAATTGG ATGTTCATCT TCGGAGGATG GAGTCTGGAT TTGGCTTCAG AATCCTCGGG
GGAGATGAGC CTGGACAGCC TATTTTGATT GGAGCTGTCA TTGCCATGGG CTCAGCCGAC
AGAGATGGCC GCCTTCACCC AGGAGATGAG CTTGTGTATG TTGATGGGAT TCCAGTAGCC
GGCAAAACCC ACCGCTATGT CATCGACCTC ATGCACCACG CAGCCCGCAA TGGGCAGGTC
AACCTCACTG TGAGAAGAAA GGTGCTATGT GGAGGGGAGC CCTGCCCAGA GAACGGGAGA
AGTCCAGGCT CTGTATCCAC CCACCACAGC TCTCCACGCA GTGACTACGC AACCTACACC
AACAGCAACC ACGCTGCCCC CAGTAGCAAT GCCTCTCCCC CTGAAGGCTT CGCCTCCCAC
AGCCTGCAGA CCAGTGATGT GGTCATTCAC CGCAAAGAGA ATGAGGGCTT CGGCTTTGTC
ATCATCAGCT CCCTGAACAG GCCTGAGTCT GGATCCACTA TAACTGTGCC CCATAAAATC
GGACGCATCA TTGATGGGAG TCCTGCAGAT CGCTGTGCAA AACTAAAAGT GGGAGACCGG
ATCCTAGCAG TGAATGGCCA GTCTATCATC AACATGCCTC ACGCTGACAT CGTGAAGCTC
ATCAAGGATG CAGGTCTTAG TGTCACCCTT CGCATCATTC CTCAGGAGGA GCTCAACAGC
CCCACCTCGG CACCCAGCTC AGAGAAGCAG AGTCCCATGG CGCAGCAGAG TCCCCTGGCA
CAGCAGAGTC CCCTGGCCCA GCCAAGCCCA GCCACCCCCA ACAGCCCCAT CGCCCAGCCA
GCACCACCTC AACCACTTCA GCTGCAAGGA CACGAAAATA GTTACAGGTC GGAAGTGAAA
GCAAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACAGACTA CAGGCAGCCC
CCGCTGGATT ACAGGCAACC CCCAGGAGGG GACTACCAGC AGCCCCCACC CTTGGACTAC
AGGCAGCCTC CCCTACTGGA CTACAGGCAG CACTCCCCCG ACACCAGGCA GTACCCTCTG
TCGGACTACA GACAACCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAATACA AAATGGATTT GTATGTGTTG
AGACTGGCAG AAGATGGACC AGCAATAAGG AATGGGAGGA TGAGGGTAGG AGATCAAATC
ATTGAAATCA ATGGGGAAAG CACAAGGGAC ATGACACATG CCAGAGCAAT AGAACTCATC
AAATCTGGAG GAAGACGAGT GAGGCTGCTG CTCAAGAGAG GCACGGGACA GGTCCCAGAA
TATGACGAAC CCGCCCCCTG GAGTTCTCCC GCTGCCGCCG CCCCAGGTCT GCCGGAAGTA
GGCGTCTCCC TGGACGACGG CCTCGCTCCA TTCTCTCCAT CACATCCAGC CCCACCCTCC
GACCCTTCCC ACCAGATAAG CCCAGGCCCA ACTTGGGATA TCAAACGGGA ACACGACGTT
AGGAAACCAA AGGAGCTTTC AGCCTGCGGC CAGAAGAAGC AGCGCCTCGG GGAGCAGAGG
GAGCGCTCGG CGAGTCCGCA GAGGGCCGCG CGGCCGAGGC TCGAGGAGGC GCCCGGCGGC
CAGGGGCGGC CCGAGGCCGG CAGGCCCGCC TCGGAGGCCA GGGCGCCCGG GCTCGCGGCG
GCAGACGCGG CGGACGCGGC GCGGGCGGGC GGGAAGGAGG CGCCCCGTGC GGCGGCGGGC
TCCGAGCTCT GCCGGCGCGA AGGCCCGGGG GCTGCGCCGG CGTTTGCCGG CCCGGGCGGC
GGCGGCAGCG GCGCGCTGGA GGCCGAGGGC AGGGCGGGTG CGCGCGCGGG TCCCCGGCCG
GGCCCGCGAC CCCCGGGGGG CGCCCCGGCG CGCAAGGCCG CCGTCGCGCC GGGGCCCTGG
AAGGTGCCGG GTTCTGACAA GCTGCCCAGC GTCCTCAAAC CCGGCGCCTC GGCCGCCAGC
AGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63410
Clone ID Related Accession (Same CDS sequence) XM_011516718.1
Accession Version XM_011516718.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515020.1
CDS486..4979
Misc Feature(1)549..773
Misc Feature(2)order(564..575,579..581,729..734,741..746)
Misc Feature(3)837..1358
Misc Feature(4)1398..1490
Misc Feature(5)order(1440..1442,1473..1475)
Misc Feature(6)1533..1622
Misc Feature(7)order(1578..1580,1611..1613)
Misc Feature(8)1767..2012
Misc Feature(9)order(1788..1799,1803..1805,1950..1955,1962..1967)
Misc Feature(10)2289..2534
Misc Feature(11)order(2325..2336,2340..2342,2469..2474,2481..2486)
Misc Feature(12)2529..2753
Misc Feature(13)2808..3068
Misc Feature(14)order(2844..2855,2859..2861,3006..3011,3018..3023)
Misc Feature(15)3243..3506
Misc Feature(16)order(3273..3284,3288..3290,3456..3461,3468..3473)
Misc Feature(17)3921..4163
Misc Feature(18)order(3951..3962,3966..3968,4113..4118,4125..4130)
Translation

Target ORF information:

RefSeq Version XM_011516718.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516718.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGTCCAAAA GCTTGAAAAA GAAAAGCCAC TGGACTAGCA AAGTCCATGA GAGTGTCATT 
GGCAGGAACC CGGAGGGCCA GCTGGGCTTT GAACTGAAGG GGGGCGCCGA GAATGGACAG
TTCCCCTACC TGGGGGAGGT GAAGCCCGGC AAGGTGGCCT ATGAGAGCGG CAGCAAATTG
GTGTCGGAGG AGCTGCTGCT GGAGGTGAAC GAGACCCCCG TGGCGGGGCT CACCATCAGG
GACGTGCTGG CCGTGATCAA ACACTGCAAG GACCCCCTCC GGCTCAAGTG TGTCAAGCAA
GGAGGAATTG TTGATAAAGA CCTTCGTCAC TACCTCAACT TACGATTTCA AAAGGGTTCT
GTGGACCATG AGCTTCAGCA AATCATTCGT GACAACCTCT ACCTCCGCAC GGTGCCATGC
ACCACAAGGC CACATAAGGA GGGTGAGGTC CCTGGAGTGG ATTATATTTT CATCACTGTT
GAAGATTTTA TGGAATTGGA GAAAAGTGGT GCTCTCCTAG AAAGTGGGAC TTATGAAGAC
AATTACTACG GTACCCCAAA GCCGCCAGCA GAACCAGCAC CATTATTGTT AAATGTAACA
GACCAGATAC TTCCAGGAGC CACTCCAAGT GCTGAAGGAA AACGGAAGAG GAATAAATCA
GTGAGCAACA TGGAGAAAGC CAGTATAGAG CCTCCTGAGG AGGAAGAGGA AGAGAGGCCT
GTGGTCAATG GAAATGGAGT AGTAGTAACA CCAGAATCCA GTGAACATGA AGACAAAAGT
GCAGGTGCCT CAGGGGAGAT GCCCTCCCAG CCTTATCCTG CACCAGTGTA CAGTCAGCCT
GAGGAGCTGA AGGAGCAGAT GGATGACACA AAGCCAACTA AACCTGAAGA CAATGAGGAA
CCAGACCCAT TGCCTGATAA CTGGGAAATG GCCTATACAG AGAAGGGCGA AGTCTACTTC
ATTGACCATA ACACAAAGAC AACATCATGG CTGGATCCAC GACTTGCGAA AAAGGCTAAA
CCTCCAGAAG AGTGCAAAGA AAATGAGCTT CCATATGGCT GGGAAAAAAT CGATGATCCC
ATTTATGGCA CTTATTATGT TGACCACATA AATAGAAGAA CACAGTTTGA AAATCCTGTC
CTGGAAGCAA AAAGGAAGCT ACAGCAACAT AACATGCCCC ACACAGAACT TGGAACAAAG
CCCCTGCAGG CCCCAGGTTT CCGAGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63411
Clone ID Related Accession (Same CDS sequence) XM_017012847.1 , XM_011516720.2 , XM_011516719.2
Accession Version XM_017012847.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TGGACATTAC CTAGGCCCAA ATCCATCCGC
CCCCGTGCTG CCCCGGAGAG GTCGCGCAGC GTGAATGACT TGTCTTACTA TCAGCGCGAC
CCAGCAGGGA GGCCCTGGCT GTTCGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868336.1
CDS7212..11345
Misc Feature(1)<7212..7595
Misc Feature(2)7314..7499
Misc Feature(3)7635..7727
Misc Feature(4)order(7677..7679,7710..7712)
Misc Feature(5)7770..7859
Misc Feature(6)order(7815..7817,7848..7850)
Misc Feature(7)8133..8378
Misc Feature(8)order(8154..8165,8169..8171,8316..8321,8328..8333)
Misc Feature(9)8655..8900
Misc Feature(10)order(8691..8702,8706..8708,8835..8840,8847..8852)
Misc Feature(11)8895..9119
Misc Feature(12)9174..9434
Misc Feature(13)order(9210..9221,9225..9227,9372..9377,9384..9389)
Misc Feature(14)9609..9872
Misc Feature(15)order(9639..9650,9654..9656,9822..9827,9834..9839)
Misc Feature(16)10287..10529
Misc Feature(17)order(10317..10328,10332..10334,10479..10484,10491..10496)
Translation

Target ORF information:

RefSeq Version XM_017012847.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012847.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TGGACATTAC CTAGGCCCAA ATCCATCCGC
CCCCGTGCTG CCCCGGAGAG GTCGCGCAGC GTGAATGACT TGTCTTACTA TCAGCGCGAC
CCAGCAGGGA GGCCCTGGCT GTTCGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63411
Clone ID Related Accession (Same CDS sequence) XM_017012847.1 , XM_011516720.2 , XM_011516719.2
Accession Version XM_011516720.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TGGACATTAC CTAGGCCCAA ATCCATCCGC
CCCCGTGCTG CCCCGGAGAG GTCGCGCAGC GTGAATGACT TGTCTTACTA TCAGCGCGAC
CCAGCAGGGA GGCCCTGGCT GTTCGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515022.1
CDS9253..13386
Misc Feature(1)<9253..9636
Misc Feature(2)9355..9540
Misc Feature(3)9676..9768
Misc Feature(4)order(9718..9720,9751..9753)
Misc Feature(5)9811..9900
Misc Feature(6)order(9856..9858,9889..9891)
Misc Feature(7)10174..10419
Misc Feature(8)order(10195..10206,10210..10212,10357..10362,10369..10374)
Misc Feature(9)10696..10941
Misc Feature(10)order(10732..10743,10747..10749,10876..10881,10888..10893)
Misc Feature(11)10936..11160
Misc Feature(12)11215..11475
Misc Feature(13)order(11251..11262,11266..11268,11413..11418,11425..11430)
Misc Feature(14)11650..11913
Misc Feature(15)order(11680..11691,11695..11697,11863..11868,11875..11880)
Misc Feature(16)12328..12570
Misc Feature(17)order(12358..12369,12373..12375,12520..12525,12532..12537)
Translation

Target ORF information:

RefSeq Version XM_011516720.2
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516720.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TGGACATTAC CTAGGCCCAA ATCCATCCGC
CCCCGTGCTG CCCCGGAGAG GTCGCGCAGC GTGAATGACT TGTCTTACTA TCAGCGCGAC
CCAGCAGGGA GGCCCTGGCT GTTCGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63411
Clone ID Related Accession (Same CDS sequence) XM_017012847.1 , XM_011516720.2 , XM_011516719.2
Accession Version XM_011516719.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516719.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TGGACATTAC CTAGGCCCAA ATCCATCCGC
CCCCGTGCTG CCCCGGAGAG GTCGCGCAGC GTGAATGACT TGTCTTACTA TCAGCGCGAC
CCAGCAGGGA GGCCCTGGCT GTTCGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515021.1
CDS4263..8396
Misc Feature(1)<4263..4646
Misc Feature(2)4365..4550
Misc Feature(3)4686..4778
Misc Feature(4)order(4728..4730,4761..4763)
Misc Feature(5)4821..4910
Misc Feature(6)order(4866..4868,4899..4901)
Misc Feature(7)5184..5429
Misc Feature(8)order(5205..5216,5220..5222,5367..5372,5379..5384)
Misc Feature(9)5706..5951
Misc Feature(10)order(5742..5753,5757..5759,5886..5891,5898..5903)
Misc Feature(11)5946..6170
Misc Feature(12)6225..6485
Misc Feature(13)order(6261..6272,6276..6278,6423..6428,6435..6440)
Misc Feature(14)6660..6923
Misc Feature(15)order(6690..6701,6705..6707,6873..6878,6885..6890)
Misc Feature(16)7338..7580
Misc Feature(17)order(7368..7379,7383..7385,7530..7535,7542..7547)
Translation

Target ORF information:

RefSeq Version XM_011516719.2
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516719.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGGAATTGG AGAAAAGTGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
GGTACCCCAA AGCCGCCAGC AGAACCAGCA CCATTATTGT TAAATGTAAC AGACCAGATA
CTTCCAGGAG CCACTCCAAG TGCTGAAGGA AAACGGAAGA GGAATAAATC AGTGAGCAAC
ATGGAGAAAG CCAGTATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
GGAAATGGAG TAGTAGTAAC ACCAGAATCC AGTGAACATG AAGACAAAAG TGCAGGTGCC
TCAGGGGAGA TGCCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC TGAGGAGCTG
AAGGAGCAGA TGGATGACAC AAAGCCAACT AAACCTGAAG ACAATGAGGA ACCAGACCCA
TTGCCTGATA ACTGGGAAAT GGCCTATACA GAGAAGGGCG AAGTCTACTT CATTGACCAT
AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATTTATGGC
ACTTATTATG TTGACCACAT AAATAGAAGA ACACAGTTTG AAAATCCTGT CCTGGAAGCA
AAAAGGAAGC TACAGCAACA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCCTGCAG
GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TGGACATTAC CTAGGCCCAA ATCCATCCGC
CCCCGTGCTG CCCCGGAGAG GTCGCGCAGC GTGAATGACT TGTCTTACTA TCAGCGCGAC
CCAGCAGGGA GGCCCTGGCT GTTCGAAAAA CCACTCTTCA CCCGGGATGC ATCCCAGTTG
AAGGGAACAT TCCTCAGCAC CACCCTAAAA AAGAGCAACA TGGGCTTTGG ATTTACCATC
ATTGGTGGAG ACGAGCCTGA TGAGTTTCTG CAGGTGAAAA GTGTGATTCC GGATGGGCCT
GCAGCACAGG ATGGAAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
GTCCTTGGAC ACACTCATGC AGATGTTGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
AGTGTCAACC TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGACCCTGCT
AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
AGACACAACT ATGAAACATA TTTGGAGTAC ATTTCTCGGA CCTCACAGTC AGTTCCAGAT
ATAACAGATC GGCCGCCTCA TTCTCTGCAC TCCATGCCAA CTGATGGTCA GCTAGACGGC
ACGTATCCAC CGCCCGTCCA TGATGACAAT GTGTCTATGG CTTCATCTGG GGCCACCCAA
GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GCTTCGGCTT CACTATTGCC
GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
TGTGAAGGCG ACCTCATTGT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACA
GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGTG AAACTTCTTT GATTATCCAT
CGAGGAGGTT TCTTTTCTCC ATGGAAAACT CCAAAGCCTA TAATGGACCG ATGGGAGAAT
CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CGCAGAACCT GCCCTTCCCA
CCTGCCCTTC ACAGGAGCTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC ACGGAAGCCT
GATCCATATG AGCTCTACGA GAAATCTAGG GCCATTTATG AAAGTAGGCA ACAAGTGCCA
CCCAGGACCA GTTTTCGAAT GGATTCCTCT GGTCCAGATT ATAAGGAATT GGATGTTCAT
CTTCGGAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
CCTATTTTGA TTGGAGCTGT CATTGCCATG GGCTCAGCCG ACAGAGATGG CCGCCTTCAC
CCAGGAGATG AGCTTGTGTA TGTTGATGGG ATTCCAGTAG CCGGCAAAAC CCACCGCTAT
GTCATCGACC TCATGCACCA CGCAGCCCGC AATGGGCAGG TCAACCTCAC TGTGAGAAGA
AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAACGGGA GAAGTCCAGG CTCTGTATCC
ACCCACCACA GCTCTCCACG CAGTGACTAC GCAACCTACA CCAACAGCAA CCACGCTGCC
CCCAGTAGCA ATGCCTCTCC CCCTGAAGGC TTCGCCTCCC ACAGCCTGCA GACCAGTGAT
GTGGTCATTC ACCGCAAAGA GAATGAGGGC TTCGGCTTTG TCATCATCAG CTCCCTGAAC
AGGCCTGAGT CTGGATCCAC TATAACTGTG CCCCATAAAA TCGGACGCAT CATTGATGGG
AGTCCTGCAG ATCGCTGTGC AAAACTAAAA GTGGGAGACC GGATCCTAGC AGTGAATGGC
CAGTCTATCA TCAACATGCC TCACGCTGAC ATCGTGAAGC TCATCAAGGA TGCAGGTCTT
AGTGTCACCC TTCGCATCAT TCCTCAGGAG GAGCTCAACA GCCCCACCTC GGCACCCAGC
TCAGAGAAGC AGAGTCCCAT GGCGCAGCAG AGTCCCCTGG CACAGCAGAG TCCCCTGGCC
CAGCCAAGCC CAGCCACCCC CAACAGCCCC ATCGCCCAGC CAGCACCACC TCAACCACTT
CAGCTGCAAG GACACGAAAA TAGTTACAGG TCGGAAGTGA AAGCAAGGCA AGATGTGAAA
CCAGACATCC GACAGCCTCC ATTCACAGAC TACAGGCAGC CCCCGCTGGA TTACAGGCAA
CCCCCAGGAG GGGACTACCA GCAGCCCCCA CCCTTGGACT ACAGGCAGCC TCCCCTACTG
GACTACAGGC AGCACTCCCC CGACACCAGG CAGTACCCTC TGTCGGACTA CAGACAACCC
CAGGATTTTG ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC
ATTCGTGGAG GAAGGGAATA CAAAATGGAT TTGTATGTGT TGAGACTGGC AGAAGATGGA
CCAGCAATAA GGAATGGGAG GATGAGGGTA GGAGATCAAA TCATTGAAAT CAATGGGGAA
AGCACAAGGG ACATGACACA TGCCAGAGCA ATAGAACTCA TCAAATCTGG AGGAAGACGA
GTGAGGCTGC TGCTCAAGAG AGGCACGGGA CAGGTCCCAG AATATGGAAT GGTACCTTCC
AGTCTCTCCA TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTATTGGGC
CACCCTAAAG ACACGGAAAG ATCCAAACTG GCTAGAACTG ACAATAGGAA GGACGAACCC
GCCCCCTGGA GTTCTCCCGC TGCCGCCGCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
GACGACGGCC TCGCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
CAGATAAGCC CAGGCCCAAC TTGGGATATC AAACGGGAAC ACGACGTTAG GAAACCAAAG
GAGCTTTCAG CCTGCGGCCA GAAGAAGCAG CGCCTCGGGG AGCAGAGGGA GCGCTCGGCG
AGTCCGCAGA GGGCCGCGCG GCCGAGGCTC GAGGAGGCGC CCGGCGGCCA GGGGCGGCCC
GAGGCCGGCA GGCCCGCCTC GGAGGCCAGG GCGCCCGGGC TCGCGGCGGC AGACGCGGCG
GACGCGGCGC GGGCGGGCGG GAAGGAGGCG CCCCGTGCGG CGGCGGGCTC CGAGCTCTGC
CGGCGCGAAG GCCCGGGGGC TGCGCCGGCG TTTGCCGGCC CGGGCGGCGG CGGCAGCGGC
GCGCTGGAGG CCGAGGGCAG GGCGGGTGCG CGCGCGGGTC CCCGGCCGGG CCCGCGACCC
CCGGGGGGCG CCCCGGCGCG CAAGGCCGCC GTCGCGCCGG GGCCCTGGAA GGTGCCGGGT
TCTGACAAGC TGCCCAGCGT CCTCAAACCC GGCGCCTCGG CCGCCAGCAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63416
Clone ID Related Accession (Same CDS sequence) XM_011516726.2
Accession Version XM_011516726.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X15
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCCCCACA CAGAACTTGG AACAAAGCCC CTGCAGGCCC CAGGTTTCCG AGTAGACAGC 
TCCTCATGGA CATTACCTAG GCCCAAATCC ATCCGCCCCC GTGCTGCCCC GGAGAGGTCG
CGCAGCGTGA ATGACTTGTC TTACTATCAG CGCGACCCAG CAGGGAGGCC CTGGCTGTTC
GAAAAACCAC TCTTCACCCG GGATGCATCC CAGTTGAAGG GAACATTCCT CAGCACCACC
CTAAAAAAGA GCAACATGGG CTTTGGATTT ACCATCATTG GTGGAGACGA GCCTGATGAG
TTTCTGCAGG TGAAAAGTGT GATTCCGGAT GGGCCTGCAG CACAGGATGG AAAAATGGAA
ACAGGTGATG TCATTGTCTA TATTAATGAA GTTTGTGTCC TTGGACACAC TCATGCAGAT
GTTGTCAAAC TTTTCCAGTC TGTTCCTATT GGTCAGAGTG TCAACCTGGT GTTGTGTCGT
GGCTACCCTT TGCCCTTTGA TCCTGAAGAC CCTGCTAACA GCATGGTGCC ACCCCTTGCA
ATAATGGAGA GGCCACCTCC AGTGATGGTC AATGGAAGAC ACAACTATGA AACATATTTG
GAGTACATTT CTCGGACCTC ACAGTCAGTT CCAGATATAA CAGATCGGCC GCCTCATTCT
CTGCACTCCA TGCCAACTGA TGGTCAGCTA GACGGCACGT ATCCACCGCC CGTCCATGAT
GACAATGTGT CTATGGCTTC ATCTGGGGCC ACCCAAGCTG AACTTATGAC CTTAACCATT
GTGAAAGGTG CCCAGGGCTT CGGCTTCACT ATTGCCGACA GTCCTACAGG ACAGCGGGTG
AAACAAATAC TTGACATTCA GGGATGCCCT GGCCTGTGTG AAGGCGACCT CATTGTTGAG
ATCAACCAGC AGAATGTACA GAACCTGAGC CATACAGAAG TAGTGGATAT ACTTAAGGAC
TGTCCCATTG GAAGTGAAAC TTCTTTGATT ATCCATCGAG GAGGTTTCTT TTCTCCATGG
AAAACTCCAA AGCCTATAAT GGACCGATGG GAGAATCAAG GCAGTCCTCA AACGAGTTTA
TCTGCTCCGG CCATACCGCA GAACCTGCCC TTCCCACCTG CCCTTCACAG GAGCTCCTTT
CCTGACTCAA CAGAGGCCTT TGACCCACGG AAGCCTGATC CATATGAGCT CTACGAGAAA
TCTAGGGCCA TTTATGAAAG TAGGCAACAA GTGCCACCCA GGACCAGTTT TCGAATGGAT
TCCTCTGGTC CAGATTATAA GGAATTGGAT GTTCATCTTC GGAGGATGGA GTCTGGATTT
GGCTTCAGAA TCCTCGGGGG AGATGAGCCT GGACAGCCTA TTTTGATTGG AGCTGTCATT
GCCATGGGCT CAGCCGACAG AGATGGCCGC CTTCACCCAG GAGATGAGCT TGTGTATGTT
GATGGGATTC CAGTAGCCGG CAAAACCCAC CGCTATGTCA TCGACCTCAT GCACCACGCA
GCCCGCAATG GGCAGGTCAA CCTCACTGTG AGAAGAAAGG TGCTATGTGG AGGGGAGCCC
TGCCCAGAGA ACGGGAGAAG TCCAGGCTCT GTATCCACCC ACCACAGCTC TCCACGCAGT
GACTACGCAA CCTACACCAA CAGCAACCAC GCTGCCCCCA GTAGCAATGC CTCTCCCCCT
GAAGGCTTCG CCTCCCACAG CCTGCAGACC AGTGATGTGG TCATTCACCG CAAAGAGAAT
GAGGGCTTCG GCTTTGTCAT CATCAGCTCC CTGAACAGGC CTGAGTCTGG ATCCACTATA
ACTGTGCCCC ATAAAATCGG ACGCATCATT GATGGGAGTC CTGCAGATCG CTGTGCAAAA
CTAAAAGTGG GAGACCGGAT CCTAGCAGTG AATGGCCAGT CTATCATCAA CATGCCTCAC
GCTGACATCG TGAAGCTCAT CAAGGATGCA GGTCTTAGTG TCACCCTTCG CATCATTCCT
CAGGAGGAGC TCAACAGCCC CACCTCGGCA CCCAGCTCAG AGAAGCAGAG TCCCATGGCG
CAGCAGAGTC CCCTGGCACA GCAGAGTCCC CTGGCCCAGC CAAGCCCAGC CACCCCCAAC
AGCCCCATCG CCCAGCCAGC ACCACCTCAA CCACTTCAGC TGCAAGGACA CGAAAATAGT
TACAGGTCGG AAGTGAAAGC AAGGCAAGAT GTGAAACCAG ACATCCGACA GCCTCCATTC
ACAGACTACA GGCAGCCCCC GCTGGATTAC AGGCAACCCC CAGGAGGGGA CTACCAGCAG
CCCCCACCCT TGGACTACAG GCAGCCTCCC CTACTGGACT ACAGGCAGCA CTCCCCCGAC
ACCAGGCAGT ACCCTCTGTC GGACTACAGA CAACCCCAGG ATTTTGATTA TTTCACTGTG
GACATGGAGA AAGGAGCCAA AGGATTTGGA TTCAGCATTC GTGGAGGAAG GGAATACAAA
ATGGATTTGT ATGTGTTGAG ACTGGCAGAA GATGGACCAG CAATAAGGAA TGGGAGGATG
AGGGTAGGAG ATCAAATCAT TGAAATCAAT GGGGAAAGCA CAAGGGACAT GACACATGCC
AGAGCAATAG AACTCATCAA ATCTGGAGGA AGACGAGTGA GGCTGCTGCT CAAGAGAGGC
ACGGGACAGG TCCCAGAATA TGGAATGGTA CCTTCCAGTC TCTCCATGTG CATGAAAAGT
GACAAGCATG GGTCCCCATA TTTCTACTTA TTGGGCCACC CTAAAGACAC GGAAAGATCC
AAACTGGCTA GAACTGACAA TAGGAAGGAC GAACCCGCCC CCTGGAGTTC TCCCGCTGCC
GCCGCCCCAG GTCTGCCGGA AGTAGGCGTC TCCCTGGACG ACGGCCTCGC TCCATTCTCT
CCATCACATC CAGCCCCACC CTCCGACCCT TCCCACCAGA TAAGCCCAGG CCCAACTTGG
GATATCAAAC GGGAACACGA CGTTAGGAAA CCAAAGGAGC TTTCAGCCTG CGGCCAGAAG
AAGCAGCGCC TCGGGGAGCA GAGGGAGCGC TCGGCGAGTC CGCAGAGGGC CGCGCGGCCG
AGGCTCGAGG AGGCGCCCGG CGGCCAGGGG CGGCCCGAGG CCGGCAGGCC CGCCTCGGAG
GCCAGGGCGC CCGGGCTCGC GGCGGCAGAC GCGGCGGACG CGGCGCGGGC GGGCGGGAAG
GAGGCGCCCC GTGCGGCGGC GGGCTCCGAG CTCTGCCGGC GCGAAGGCCC GGGGGCTGCG
CCGGCGTTTG CCGGCCCGGG CGGCGGCGGC AGCGGCGCGC TGGAGGCCGA GGGCAGGGCG
GGTGCGCGCG CGGGTCCCCG GCCGGGCCCG CGACCCCCGG GGGGCGCCCC GGCGCGCAAG
GCCGCCGTCG CGCCGGGGCC CTGGAAGGTG CCGGGTTCTG ACAAGCTGCC CAGCGTCCTC
AAACCCGGCG CCTCGGCCGC CAGCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515028.1
CDS603..4052
Misc Feature(1)840..1085
Misc Feature(2)order(861..872,876..878,1023..1028,1035..1040)
Misc Feature(3)1362..1607
Misc Feature(4)order(1398..1409,1413..1415,1542..1547,1554..1559)
Misc Feature(5)1602..1826
Misc Feature(6)1881..2141
Misc Feature(7)order(1917..1928,1932..1934,2079..2084,2091..2096)
Misc Feature(8)2316..2579
Misc Feature(9)order(2346..2357,2361..2363,2529..2534,2541..2546)
Misc Feature(10)2994..3236
Misc Feature(11)order(3024..3035,3039..3041,3186..3191,3198..3203)
Translation

Target ORF information:

RefSeq Version XM_011516726.2
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516726.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCCCCACA CAGAACTTGG AACAAAGCCC CTGCAGGCCC CAGGTTTCCG AGTAGACAGC 
TCCTCATGGA CATTACCTAG GCCCAAATCC ATCCGCCCCC GTGCTGCCCC GGAGAGGTCG
CGCAGCGTGA ATGACTTGTC TTACTATCAG CGCGACCCAG CAGGGAGGCC CTGGCTGTTC
GAAAAACCAC TCTTCACCCG GGATGCATCC CAGTTGAAGG GAACATTCCT CAGCACCACC
CTAAAAAAGA GCAACATGGG CTTTGGATTT ACCATCATTG GTGGAGACGA GCCTGATGAG
TTTCTGCAGG TGAAAAGTGT GATTCCGGAT GGGCCTGCAG CACAGGATGG AAAAATGGAA
ACAGGTGATG TCATTGTCTA TATTAATGAA GTTTGTGTCC TTGGACACAC TCATGCAGAT
GTTGTCAAAC TTTTCCAGTC TGTTCCTATT GGTCAGAGTG TCAACCTGGT GTTGTGTCGT
GGCTACCCTT TGCCCTTTGA TCCTGAAGAC CCTGCTAACA GCATGGTGCC ACCCCTTGCA
ATAATGGAGA GGCCACCTCC AGTGATGGTC AATGGAAGAC ACAACTATGA AACATATTTG
GAGTACATTT CTCGGACCTC ACAGTCAGTT CCAGATATAA CAGATCGGCC GCCTCATTCT
CTGCACTCCA TGCCAACTGA TGGTCAGCTA GACGGCACGT ATCCACCGCC CGTCCATGAT
GACAATGTGT CTATGGCTTC ATCTGGGGCC ACCCAAGCTG AACTTATGAC CTTAACCATT
GTGAAAGGTG CCCAGGGCTT CGGCTTCACT ATTGCCGACA GTCCTACAGG ACAGCGGGTG
AAACAAATAC TTGACATTCA GGGATGCCCT GGCCTGTGTG AAGGCGACCT CATTGTTGAG
ATCAACCAGC AGAATGTACA GAACCTGAGC CATACAGAAG TAGTGGATAT ACTTAAGGAC
TGTCCCATTG GAAGTGAAAC TTCTTTGATT ATCCATCGAG GAGGTTTCTT TTCTCCATGG
AAAACTCCAA AGCCTATAAT GGACCGATGG GAGAATCAAG GCAGTCCTCA AACGAGTTTA
TCTGCTCCGG CCATACCGCA GAACCTGCCC TTCCCACCTG CCCTTCACAG GAGCTCCTTT
CCTGACTCAA CAGAGGCCTT TGACCCACGG AAGCCTGATC CATATGAGCT CTACGAGAAA
TCTAGGGCCA TTTATGAAAG TAGGCAACAA GTGCCACCCA GGACCAGTTT TCGAATGGAT
TCCTCTGGTC CAGATTATAA GGAATTGGAT GTTCATCTTC GGAGGATGGA GTCTGGATTT
GGCTTCAGAA TCCTCGGGGG AGATGAGCCT GGACAGCCTA TTTTGATTGG AGCTGTCATT
GCCATGGGCT CAGCCGACAG AGATGGCCGC CTTCACCCAG GAGATGAGCT TGTGTATGTT
GATGGGATTC CAGTAGCCGG CAAAACCCAC CGCTATGTCA TCGACCTCAT GCACCACGCA
GCCCGCAATG GGCAGGTCAA CCTCACTGTG AGAAGAAAGG TGCTATGTGG AGGGGAGCCC
TGCCCAGAGA ACGGGAGAAG TCCAGGCTCT GTATCCACCC ACCACAGCTC TCCACGCAGT
GACTACGCAA CCTACACCAA CAGCAACCAC GCTGCCCCCA GTAGCAATGC CTCTCCCCCT
GAAGGCTTCG CCTCCCACAG CCTGCAGACC AGTGATGTGG TCATTCACCG CAAAGAGAAT
GAGGGCTTCG GCTTTGTCAT CATCAGCTCC CTGAACAGGC CTGAGTCTGG ATCCACTATA
ACTGTGCCCC ATAAAATCGG ACGCATCATT GATGGGAGTC CTGCAGATCG CTGTGCAAAA
CTAAAAGTGG GAGACCGGAT CCTAGCAGTG AATGGCCAGT CTATCATCAA CATGCCTCAC
GCTGACATCG TGAAGCTCAT CAAGGATGCA GGTCTTAGTG TCACCCTTCG CATCATTCCT
CAGGAGGAGC TCAACAGCCC CACCTCGGCA CCCAGCTCAG AGAAGCAGAG TCCCATGGCG
CAGCAGAGTC CCCTGGCACA GCAGAGTCCC CTGGCCCAGC CAAGCCCAGC CACCCCCAAC
AGCCCCATCG CCCAGCCAGC ACCACCTCAA CCACTTCAGC TGCAAGGACA CGAAAATAGT
TACAGGTCGG AAGTGAAAGC AAGGCAAGAT GTGAAACCAG ACATCCGACA GCCTCCATTC
ACAGACTACA GGCAGCCCCC GCTGGATTAC AGGCAACCCC CAGGAGGGGA CTACCAGCAG
CCCCCACCCT TGGACTACAG GCAGCCTCCC CTACTGGACT ACAGGCAGCA CTCCCCCGAC
ACCAGGCAGT ACCCTCTGTC GGACTACAGA CAACCCCAGG ATTTTGATTA TTTCACTGTG
GACATGGAGA AAGGAGCCAA AGGATTTGGA TTCAGCATTC GTGGAGGAAG GGAATACAAA
ATGGATTTGT ATGTGTTGAG ACTGGCAGAA GATGGACCAG CAATAAGGAA TGGGAGGATG
AGGGTAGGAG ATCAAATCAT TGAAATCAAT GGGGAAAGCA CAAGGGACAT GACACATGCC
AGAGCAATAG AACTCATCAA ATCTGGAGGA AGACGAGTGA GGCTGCTGCT CAAGAGAGGC
ACGGGACAGG TCCCAGAATA TGGAATGGTA CCTTCCAGTC TCTCCATGTG CATGAAAAGT
GACAAGCATG GGTCCCCATA TTTCTACTTA TTGGGCCACC CTAAAGACAC GGAAAGATCC
AAACTGGCTA GAACTGACAA TAGGAAGGAC GAACCCGCCC CCTGGAGTTC TCCCGCTGCC
GCCGCCCCAG GTCTGCCGGA AGTAGGCGTC TCCCTGGACG ACGGCCTCGC TCCATTCTCT
CCATCACATC CAGCCCCACC CTCCGACCCT TCCCACCAGA TAAGCCCAGG CCCAACTTGG
GATATCAAAC GGGAACACGA CGTTAGGAAA CCAAAGGAGC TTTCAGCCTG CGGCCAGAAG
AAGCAGCGCC TCGGGGAGCA GAGGGAGCGC TCGGCGAGTC CGCAGAGGGC CGCGCGGCCG
AGGCTCGAGG AGGCGCCCGG CGGCCAGGGG CGGCCCGAGG CCGGCAGGCC CGCCTCGGAG
GCCAGGGCGC CCGGGCTCGC GGCGGCAGAC GCGGCGGACG CGGCGCGGGC GGGCGGGAAG
GAGGCGCCCC GTGCGGCGGC GGGCTCCGAG CTCTGCCGGC GCGAAGGCCC GGGGGCTGCG
CCGGCGTTTG CCGGCCCGGG CGGCGGCGGC AGCGGCGCGC TGGAGGCCGA GGGCAGGGCG
GGTGCGCGCG CGGGTCCCCG GCCGGGCCCG CGACCCCCGG GGGGCGCCCC GGCGCGCAAG
GCCGCCGTCG CGCCGGGGCC CTGGAAGGTG CCGGGTTCTG ACAAGCTGCC CAGCGTCCTC
AAACCCGGCG CCTCGGCCGC CAGCAGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63417
Clone ID Related Accession (Same CDS sequence) XM_011516728.1
Accession Version XM_011516728.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X16
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCCCCACA CAGAACTTGG AACAAAGCCC CTGCAGGCCC CAGGTTTCCG AGAAAAACCA 
CTCTTCACCC GGGATGCATC CCAGTTGAAG GGAACATTCC TCAGCACCAC CCTAAAAAAG
AGCAACATGG GCTTTGGATT TACCATCATT GGTGGAGACG AGCCTGATGA GTTTCTGCAG
GTGAAAAGTG TGATTCCGGA TGGGCCTGCA GCACAGGATG GAAAAATGGA AACAGGTGAT
GTCATTGTCT ATATTAATGA AGTTTGTGTC CTTGGACACA CTCATGCAGA TGTTGTCAAA
CTTTTCCAGT CTGTTCCTAT TGGTCAGAGT GTCAACCTGG TGTTGTGTCG TGGCTACCCT
TTGCCCTTTG ATCCTGAAGA CCCTGCTAAC AGCATGGTGC CACCCCTTGC AATAATGGAG
AGGCCACCTC CAGTGATGGT CAATGGAAGA CACAACTATG AAACATATTT GGAGTACATT
TCTCGGACCT CACAGTCAGT TCCAGATATA ACAGATCGGC CGCCTCATTC TCTGCACTCC
ATGCCAACTG ATGGTCAGCT AGACGGCACG TATCCACCGC CCGTCCATGA TGACAATGTG
TCTATGGCTT CATCTGGGGC CACCCAAGCT GAACTTATGA CCTTAACCAT TGTGAAAGGT
GCCCAGGGCT TCGGCTTCAC TATTGCCGAC AGTCCTACAG GACAGCGGGT GAAACAAATA
CTTGACATTC AGGGATGCCC TGGCCTGTGT GAAGGCGACC TCATTGTTGA GATCAACCAG
CAGAATGTAC AGAACCTGAG CCATACAGAA GTAGTGGATA TACTTAAGGA CTGTCCCATT
GGAAGTGAAA CTTCTTTGAT TATCCATCGA GGAGGTTTCT TTTCTCCATG GAAAACTCCA
AAGCCTATAA TGGACCGATG GGAGAATCAA GGCAGTCCTC AAACGAGTTT ATCTGCTCCG
GCCATACCGC AGAACCTGCC CTTCCCACCT GCCCTTCACA GGAGCTCCTT TCCTGACTCA
ACAGAGGCCT TTGACCCACG GAAGCCTGAT CCATATGAGC TCTACGAGAA ATCTAGGGCC
ATTTATGAAA GTAGGCAACA AGTGCCACCC AGGACCAGTT TTCGAATGGA TTCCTCTGGT
CCAGATTATA AGGAATTGGA TGTTCATCTT CGGAGGATGG AGTCTGGATT TGGCTTCAGA
ATCCTCGGGG GAGATGAGCC TGGACAGCCT ATTTTGATTG GAGCTGTCAT TGCCATGGGC
TCAGCCGACA GAGATGGCCG CCTTCACCCA GGAGATGAGC TTGTGTATGT TGATGGGATT
CCAGTAGCCG GCAAAACCCA CCGCTATGTC ATCGACCTCA TGCACCACGC AGCCCGCAAT
GGGCAGGTCA ACCTCACTGT GAGAAGAAAG GTGCTATGTG GAGGGGAGCC CTGCCCAGAG
AACGGGAGAA GTCCAGGCTC TGTATCCACC CACCACAGCT CTCCACGCAG TGACTACGCA
ACCTACACCA ACAGCAACCA CGCTGCCCCC AGTAGCAATG CCTCTCCCCC TGAAGGCTTC
GCCTCCCACA GCCTGCAGAC CAGTGATGTG GTCATTCACC GCAAAGAGAA TGAGGGCTTC
GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCTGAGTCTG GATCCACTAT AACTGTGCCC
CATAAAATCG GACGCATCAT TGATGGGAGT CCTGCAGATC GCTGTGCAAA ACTAAAAGTG
GGAGACCGGA TCCTAGCAGT GAATGGCCAG TCTATCATCA ACATGCCTCA CGCTGACATC
GTGAAGCTCA TCAAGGATGC AGGTCTTAGT GTCACCCTTC GCATCATTCC TCAGGAGGAG
CTCAACAGCC CCACCTCGGC ACCCAGCTCA GAGAAGCAGA GTCCCATGGC GCAGCAGAGT
CCCCTGGCAC AGCAGAGTCC CCTGGCCCAG CCAAGCCCAG CCACCCCCAA CAGCCCCATC
GCCCAGCCAG CACCACCTCA ACCACTTCAG CTGCAAGGAC ACGAAAATAG TTACAGGTCG
GAAGTGAAAG CAAGGCAAGA TGTGAAACCA GACATCCGAC AGCCTCCATT CACAGACTAC
AGGCAGCCCC CGCTGGATTA CAGGCAACCC CCAGGAGGGG ACTACCAGCA GCCCCCACCC
TTGGACTACA GGCAGCCTCC CCTACTGGAC TACAGGCAGC ACTCCCCCGA CACCAGGCAG
TACCCTCTGT CGGACTACAG ACAACCCCAG GATTTTGATT ATTTCACTGT GGACATGGAG
AAAGGAGCCA AAGGATTTGG ATTCAGCATT CGTGGAGGAA GGGAATACAA AATGGATTTG
TATGTGTTGA GACTGGCAGA AGATGGACCA GCAATAAGGA ATGGGAGGAT GAGGGTAGGA
GATCAAATCA TTGAAATCAA TGGGGAAAGC ACAAGGGACA TGACACATGC CAGAGCAATA
GAACTCATCA AATCTGGAGG AAGACGAGTG AGGCTGCTGC TCAAGAGAGG CACGGGACAG
GTCCCAGAAT ATGGAATGGT ACCTTCCAGT CTCTCCATGT GCATGAAAAG TGACAAGCAT
GGGTCCCCAT ATTTCTACTT ATTGGGCCAC CCTAAAGACA CGGAAAGATC CAAACTGGCT
AGAACTGACA ATAGGAAGGA CGAACCCGCC CCCTGGAGTT CTCCCGCTGC CGCCGCCCCA
GGTCTGCCGG AAGTAGGCGT CTCCCTGGAC GACGGCCTCG CTCCATTCTC TCCATCACAT
CCAGCCCCAC CCTCCGACCC TTCCCACCAG ATAAGCCCAG GCCCAACTTG GGATATCAAA
CGGGAACACG ACGTTAGGAA ACCAAAGGAG CTTTCAGCCT GCGGCCAGAA GAAGCAGCGC
CTCGGGGAGC AGAGGGAGCG CTCGGCGAGT CCGCAGAGGG CCGCGCGGCC GAGGCTCGAG
GAGGCGCCCG GCGGCCAGGG GCGGCCCGAG GCCGGCAGGC CCGCCTCGGA GGCCAGGGCG
CCCGGGCTCG CGGCGGCAGA CGCGGCGGAC GCGGCGCGGG CGGGCGGGAA GGAGGCGCCC
CGTGCGGCGG CGGGCTCCGA GCTCTGCCGG CGCGAAGGCC CGGGGGCTGC GCCGGCGTTT
GCCGGCCCGG GCGGCGGCGG CAGCGGCGCG CTGGAGGCCG AGGGCAGGGC GGGTGCGCGC
GCGGGTCCCC GGCCGGGCCC GCGACCCCCG GGGGGCGCCC CGGCGCGCAA GGCCGCCGTC
GCGCCGGGGC CCTGGAAGGT GCCGGGTTCT GACAAGCTGC CCAGCGTCCT CAAACCCGGC
GCCTCGGCCG CCAGCAGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515030.1
CDS268..3588
Misc Feature(1)376..621
Misc Feature(2)order(397..408,412..414,559..564,571..576)
Misc Feature(3)898..1143
Misc Feature(4)order(934..945,949..951,1078..1083,1090..1095)
Misc Feature(5)1138..1362
Misc Feature(6)1417..1677
Misc Feature(7)order(1453..1464,1468..1470,1615..1620,1627..1632)
Misc Feature(8)1852..2115
Misc Feature(9)order(1882..1893,1897..1899,2065..2070,2077..2082)
Misc Feature(10)2530..2772
Misc Feature(11)order(2560..2571,2575..2577,2722..2727,2734..2739)
Translation

Target ORF information:

RefSeq Version XM_011516728.1
Organism Homo sapiens(Human)
Definition Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011516728.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCCCCACA CAGAACTTGG AACAAAGCCC CTGCAGGCCC CAGGTTTCCG AGAAAAACCA 
CTCTTCACCC GGGATGCATC CCAGTTGAAG GGAACATTCC TCAGCACCAC CCTAAAAAAG
AGCAACATGG GCTTTGGATT TACCATCATT GGTGGAGACG AGCCTGATGA GTTTCTGCAG
GTGAAAAGTG TGATTCCGGA TGGGCCTGCA GCACAGGATG GAAAAATGGA AACAGGTGAT
GTCATTGTCT ATATTAATGA AGTTTGTGTC CTTGGACACA CTCATGCAGA TGTTGTCAAA
CTTTTCCAGT CTGTTCCTAT TGGTCAGAGT GTCAACCTGG TGTTGTGTCG TGGCTACCCT
TTGCCCTTTG ATCCTGAAGA CCCTGCTAAC AGCATGGTGC CACCCCTTGC AATAATGGAG
AGGCCACCTC CAGTGATGGT CAATGGAAGA CACAACTATG AAACATATTT GGAGTACATT
TCTCGGACCT CACAGTCAGT TCCAGATATA ACAGATCGGC CGCCTCATTC TCTGCACTCC
ATGCCAACTG ATGGTCAGCT AGACGGCACG TATCCACCGC CCGTCCATGA TGACAATGTG
TCTATGGCTT CATCTGGGGC CACCCAAGCT GAACTTATGA CCTTAACCAT TGTGAAAGGT
GCCCAGGGCT TCGGCTTCAC TATTGCCGAC AGTCCTACAG GACAGCGGGT GAAACAAATA
CTTGACATTC AGGGATGCCC TGGCCTGTGT GAAGGCGACC TCATTGTTGA GATCAACCAG
CAGAATGTAC AGAACCTGAG CCATACAGAA GTAGTGGATA TACTTAAGGA CTGTCCCATT
GGAAGTGAAA CTTCTTTGAT TATCCATCGA GGAGGTTTCT TTTCTCCATG GAAAACTCCA
AAGCCTATAA TGGACCGATG GGAGAATCAA GGCAGTCCTC AAACGAGTTT ATCTGCTCCG
GCCATACCGC AGAACCTGCC CTTCCCACCT GCCCTTCACA GGAGCTCCTT TCCTGACTCA
ACAGAGGCCT TTGACCCACG GAAGCCTGAT CCATATGAGC TCTACGAGAA ATCTAGGGCC
ATTTATGAAA GTAGGCAACA AGTGCCACCC AGGACCAGTT TTCGAATGGA TTCCTCTGGT
CCAGATTATA AGGAATTGGA TGTTCATCTT CGGAGGATGG AGTCTGGATT TGGCTTCAGA
ATCCTCGGGG GAGATGAGCC TGGACAGCCT ATTTTGATTG GAGCTGTCAT TGCCATGGGC
TCAGCCGACA GAGATGGCCG CCTTCACCCA GGAGATGAGC TTGTGTATGT TGATGGGATT
CCAGTAGCCG GCAAAACCCA CCGCTATGTC ATCGACCTCA TGCACCACGC AGCCCGCAAT
GGGCAGGTCA ACCTCACTGT GAGAAGAAAG GTGCTATGTG GAGGGGAGCC CTGCCCAGAG
AACGGGAGAA GTCCAGGCTC TGTATCCACC CACCACAGCT CTCCACGCAG TGACTACGCA
ACCTACACCA ACAGCAACCA CGCTGCCCCC AGTAGCAATG CCTCTCCCCC TGAAGGCTTC
GCCTCCCACA GCCTGCAGAC CAGTGATGTG GTCATTCACC GCAAAGAGAA TGAGGGCTTC
GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCTGAGTCTG GATCCACTAT AACTGTGCCC
CATAAAATCG GACGCATCAT TGATGGGAGT CCTGCAGATC GCTGTGCAAA ACTAAAAGTG
GGAGACCGGA TCCTAGCAGT GAATGGCCAG TCTATCATCA ACATGCCTCA CGCTGACATC
GTGAAGCTCA TCAAGGATGC AGGTCTTAGT GTCACCCTTC GCATCATTCC TCAGGAGGAG
CTCAACAGCC CCACCTCGGC ACCCAGCTCA GAGAAGCAGA GTCCCATGGC GCAGCAGAGT
CCCCTGGCAC AGCAGAGTCC CCTGGCCCAG CCAAGCCCAG CCACCCCCAA CAGCCCCATC
GCCCAGCCAG CACCACCTCA ACCACTTCAG CTGCAAGGAC ACGAAAATAG TTACAGGTCG
GAAGTGAAAG CAAGGCAAGA TGTGAAACCA GACATCCGAC AGCCTCCATT CACAGACTAC
AGGCAGCCCC CGCTGGATTA CAGGCAACCC CCAGGAGGGG ACTACCAGCA GCCCCCACCC
TTGGACTACA GGCAGCCTCC CCTACTGGAC TACAGGCAGC ACTCCCCCGA CACCAGGCAG
TACCCTCTGT CGGACTACAG ACAACCCCAG GATTTTGATT ATTTCACTGT GGACATGGAG
AAAGGAGCCA AAGGATTTGG ATTCAGCATT CGTGGAGGAA GGGAATACAA AATGGATTTG
TATGTGTTGA GACTGGCAGA AGATGGACCA GCAATAAGGA ATGGGAGGAT GAGGGTAGGA
GATCAAATCA TTGAAATCAA TGGGGAAAGC ACAAGGGACA TGACACATGC CAGAGCAATA
GAACTCATCA AATCTGGAGG AAGACGAGTG AGGCTGCTGC TCAAGAGAGG CACGGGACAG
GTCCCAGAAT ATGGAATGGT ACCTTCCAGT CTCTCCATGT GCATGAAAAG TGACAAGCAT
GGGTCCCCAT ATTTCTACTT ATTGGGCCAC CCTAAAGACA CGGAAAGATC CAAACTGGCT
AGAACTGACA ATAGGAAGGA CGAACCCGCC CCCTGGAGTT CTCCCGCTGC CGCCGCCCCA
GGTCTGCCGG AAGTAGGCGT CTCCCTGGAC GACGGCCTCG CTCCATTCTC TCCATCACAT
CCAGCCCCAC CCTCCGACCC TTCCCACCAG ATAAGCCCAG GCCCAACTTG GGATATCAAA
CGGGAACACG ACGTTAGGAA ACCAAAGGAG CTTTCAGCCT GCGGCCAGAA GAAGCAGCGC
CTCGGGGAGC AGAGGGAGCG CTCGGCGAGT CCGCAGAGGG CCGCGCGGCC GAGGCTCGAG
GAGGCGCCCG GCGGCCAGGG GCGGCCCGAG GCCGGCAGGC CCGCCTCGGA GGCCAGGGCG
CCCGGGCTCG CGGCGGCAGA CGCGGCGGAC GCGGCGCGGG CGGGCGGGAA GGAGGCGCCC
CGTGCGGCGG CGGGCTCCGA GCTCTGCCGG CGCGAAGGCC CGGGGGCTGC GCCGGCGTTT
GCCGGCCCGG GCGGCGGCGG CAGCGGCGCG CTGGAGGCCG AGGGCAGGGC GGGTGCGCGC
GCGGGTCCCC GGCCGGGCCC GCGACCCCCG GGGGGCGCCC CGGCGCGCAA GGCCGCCGTC
GCGCCGGGGC CCTGGAAGGT GCCGGGTTCT GACAAGCTGC CCAGCGTCCT CAAACCCGGC
GCCTCGGCCG CCAGCAGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

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American journal of respiratory and critical care medicine191(5)547-56(2015 Mar)
Dijkstra AE,Postma DS,van Ginneken B,Wielpütz MO,Schmidt M,Becker N,Owsijewitsch M,Kauczor HU,de Koning HJ,Lammers JW,Oudkerk M,Brandsma CA,Bossé Y,Nickle DC,Sin DD,Hiemstra PS,Wijmenga C,Smolonska J,Zanen P,Vonk JM,van den Berge M,Boezen HM,Groen HJ


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Genome-wide association study identifies multiple loci associated with both mammographic density and breast cancer risk.
Nature communications55303(2014 Oct)
Lindström S,Thompson DJ,Paterson AD,Li J,Gierach GL,Scott C,Stone J,Douglas JA,dos-Santos-Silva I,Fernandez-Navarro P,Verghase J,Smith P,Brown J,Luben R,Wareham NJ,Loos RJ,Heit JA,Pankratz VS,Norman A,Goode EL,Cunningham JM,deAndrade M,Vierkant RA,Czene K,Fasching PA,Baglietto L,Southey MC,Giles GG,Shah KP,Chan HP,Helvie MA,Beck AH,Knoblauch NW,Hazra A,Hunter DJ,Kraft P,Pollan M,Figueroa JD,Couch FJ,Hopper JL,Hall P,Easton DF,Boyd NF,Vachon CM,Tamimi RM


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Molecular biology reports41(9)6125-31(2014 Sep)
Mahdian R,Nodouzi V,Asgari M,Rezaie M,Alizadeh J,Yousefi B,Shahrokh H,Abolhasani M,Nowroozi M


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Human molecular genetics23(15)3923-42(2014 Aug)
Bauß K,Knapp B,Jores P,Roepman R,Kremer H,Wijk EV,Märker T,Wolfrum U


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PloS one8(11)e79629(2013)
Deng X,Sabino EC,Cunha-Neto E,Ribeiro AL,Ianni B,Mady C,Busch MP,Seielstad M,


 
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