LOC8042599 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8042599
Entrez Gene ID	8042599
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029982552.1	XP_029838412.1	uncharacterized protein LOC8042599 isoform X3
XM_002415629.1	XP_002415674.1	hypothetical protein
XM_029982555.1	XP_029838415.1	uncharacterized protein LOC8042599 isoform X5
XM_029982556.1	XP_029838416.1	uncharacterized protein LOC8042599 isoform X5
XM_029982553.1	XP_029838413.1	uncharacterized protein LOC8042599 isoform X4
XM_029982550.1	XP_029838410.1	uncharacterized protein LOC8042599 isoform X1
XM_029982551.1	XP_029838411.1	uncharacterized protein LOC8042599 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8042599 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8042599 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa25173	XM_029982551.1 Latest version!	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa25174	XM_029982552.1 Latest version!	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa25175	XM_029982553.1 Latest version!	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa25176	XM_029982556.1 Latest version!	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa25176	XM_029982555.1 Latest version!	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa25172	XM_029982550.1 Latest version!	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OIa10828	XM_002415629.1 Latest version!	Ixodes scapularis hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8042599 ( XM_029982551.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838411.1 Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIa25173
Clone ID Related Accession (Same CDS sequence)	XM_029982551.1
Accession Version	XM_029982551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8042599 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919951.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACCC CCGTAGACAA GATGGCGGCG CAGCACCCGG CGTCCCAGTT CGGCAGTCCG 
ATGCAGCATC ATCCCTTCGC CGACTTCCCC CCGCACCACG CGGTGTTCTT TGCGCCCGAC 
AAGGGCAACG CCGAAGAGCC GTCCTTCCAG GTGCCCTCCG TGTCGTCCAC CACGACTAGG 
CACACGCTGC TGCCCATGGT GCCGCTCCAG GAGCCCTGGG GAGACCAGCC GCCCATGGCC 
AGGAACGGCC TGGTGATGCA CCACCAGCAG CCGCAGCAGC AGCCGCCTCA CCACCACCAC 
CAGGGCGGGC CGCCCCAGCA GCAGCAGCAA CAGCAGCAGC AGCCCGGCAC CCCGGACGGC 
ATGGAGAAGG GCACCAAGAA GAAACGCAAA CGCTGCGGCG AGTGCCCTGG TTGCCTCAAG 
AAGGATAACT GCGGAGAGTG CGGGCCGTGC AGGAGTGTGC GCAGTCACCA GATCTGCAAG 
ATGCGGAAGT GCGATCAGCT CAAGACCAAA AAGGAGAAAG TAGCGAGCGC CATTTGCGGA 
AGCGGGGTCG CCGGAAAAGA CGGCATGGCG GCCTCTCCGG GCAACCGAAC TAATCTCAAC 
GGGCGGGCGG TGAACGGCGG CGGCGCTTCG ACGTCGCCCT ACCTCCCGCA GGGAGTGCCG 
GAACACCTCG GACATCCTGG GATCGGTGGC TTTCCCGGTC ACAGCGAGTA CCAGCTCCCG 
CCCACGCAAA AGCCTGCCCC GCCGGCCCAG GCCGGCCAGA TGTACGAGAT GGGGCCGCAC 
TTCGGGGCCT TCCCGGGCCA CCCGTCTCAG GCCCTGGCTT TCAATGGCCA CATGGCACAG 
CACGACGCTG GCCGCCTGGG GCTCCCCGGC GAAGAGGGCG ACTTCCCCAC GCGCCACCAG 
ATGATGAACA GCCGTCTCAA GACACTGATT CAGACTAGGC AGAGCCAGAA GGAGCAGGGT 
CCCTTCCAGG GGCTCGAGAC GACCCTAGGC CCCTCCGTCG GAGGAAGCTT TGTGCACCCG 
CAGGCCGATG CGACGGCGAC TGCCTCCCGG GGGTTCCCGC CGCTTGTGCG GAGCAACGGT 
GGCTGCGGTG CCGTGAGCGG CAACAACAAC GGCGGAAACA ATGGCGGCGG CAACAACAAC 
AACAACGGCA CCAACAACGG CGGTGGTAAC AACGGCAACG GCGGCACGGG CGGCGGTAAC 
AACAATCCGA GGCCTCTGTC GTCAAACGAG ATGCCCGGGG ATGCGTGGCA CGATGCGAGC 
CCGTCCTCGG GTCCTCCGCC GGGCAGGGCT GGTGTCGCAG AGCTACCGGA GTTTGCCAAG 
AACGGGTCCT CTTCCTCCTC CCAGGGAAGC AACAACAACG CCAACAACAA CAACTCCGAA 
GCTGAGTTCT CCCAGCCTGA GGATCAGAGT CACGAGAGTG AGTTTGCAGA GGGTGGCGCT 
GCGGAAACCA GGGGAGGTGG CGGCGGCGAC AGGTCGAGCG CTCCCCCCAA CGGAATGTTG 
CCCTACGGAA GCTTCTATGG GGCGCAAGAG GCGGCAGAAG TCCGGAAGGG TGGCGAGGGC 
AAGGTCCCCC CGCCGACAAC GAGCGCGGCG ACGACCACTT CGGCGGCGGA CGTTCACGGT 
GGTGGTGCGC ATTACCAGTT CACGTCGGCA GCGACTTCGA TGAGCACGAC GACGACGAGT 
GCCTCCGTTC ACGGCAAACC CTGCAACGGA CCAACGCAGC CTTCGTTTGC GGAGCGGCAG 
TCGCCCAGGG GCGGCCCGTT CTCCGGTGCA CCGACGTCAT TCGCTCCGGC GCCCCACTTG 
GCGAGTATGC ACCAAGAGCG CCCGGTCCCC GAAAGTCCGA CGCAGCTCCG CAACGAGGTA 
GGCGAAGCCG GCAGCGGCGG CCAGACGCGC GGCAATGAGA GCGAATCCAC GGACGCGGCG 
GCAGTCACAT CGCAGCCTTT TTTATCGAAT ACGACCACAT CTATGAATAC ATATACAAGT 
ATTATCAATA CATATAACAG CTTCGCCGCT TCGTGCGCGC CGTCCTTCTT GGCCGAGCAC 
CAGCAACCCA AGTTTGGCGG GAGCGTGGAG GATGCGGCTC GAACGGGGGG AGGAGACGCA 
TCCGACGTAG ATGGTGGTCG TATTGCCGTC GGCGAATTCC GTGGACTCGA CTTTGGGGCA 
CCCCCCCTGC CTCACCACCC CGGTGGCCCC GAGGGCCCCA GGCCGCACTA CCCCCAGTAC 
GGGGGGGCGC CTGCCGCGTT TGTGCCAGAG GGTCCTTACG CTGTTCCCTA TGGGGCCGGG 
AACCTGGGGA ATCTGGGGAA CCCGTTCCCC TTGCCCCCCA TGGGCGCCGG AGGTCCTCCC 
TTTGCGCCCA TGAGCCTCCC TGGTGCCGAC CCCTGGTGGG AGCGGCTGGA GCGGCTACGC 
AGCAACGCCA AGGCGGAGCC TCCGGCCTGC GACTGCTATG GCGCGGATGA GACGCCTCCC 
GTGGATAAAT CCCCATACTA TACCCACCTT GGTGCTGGAC CGACTGTGGC TGCTATTAGA 
GAGATGCTTG AGCACAGGCT GAACGAACGG GGCCCTGCGT TGCGCATCGA GAAGGTGCTC 
TACTCGGGCA AGGAGGGCAA GACGAGCCAG GGCTGCCCGG TGGCCAAGTG GATCATCCGG 
CGCAGTGGCC CTTCGGAGAA GGTGCTGGCC GTGCTGCGGC ACCGACCGGG CCACCGCTGC 
TTGTCGGCCT ACATCGTGAT GGCCATCGTC GCCTGGGAGG GCGTCCAGGC AGACATGGCA 
GACGACCTCT ACAGGACCGT GACCCACAAG ACCGTCAACT TTGGCTTCCC CACTCAGCGG 
AGATGTGGCA CCAACGAACA ACGAACCTGC GCCTGTCAAG GTGCCGACGC CGAGAACTGT 
GGCGCTTCCT TTTCATTTGG CTGCTCTTGG AGCATGTATT ACAATGGCTG CAAGTATGCA 
CGGAGCAAGG CTGTCCGCAA GTTCAAGCTC TCAGAACAGT CCCAGGAGCA AGAGCTTGAA 
GACAAAATGC AGATGCTCGC CACTGAAATG GCACCGCTGT ATGCAAGGGT GGCTCCGGAA 
TCCTACAAAA ACCAGACCGA GTTTGAGAAC GAAGGGATAT CCTGCAGACT AGGGTTGAAG 
CAGGGAAGAC CCTTTTCTGG CGTCACGGCG TGTGTCGACT TCTGTGCCCA TGCGCACAAG 
GACCTACACA ACATGAATAA CGGCTGTACA GTTGTTGTAA CCCTAACGAG ACATCGAGGA 
TTTGACAAGG GCGACGACGA ACAGCTGCAC GTGCTTCCAC TCTACATACT GGACGCAACC 
GACGAGCAGG GTGGCAAAGA AGGCTTTCAT GAAAAGATCA AGAATGGGTC ACTCGAGGTT 
TTGTCGAAAT TTCACACGAC GTCCAGGATA AGAAAGGTCC CCTTGGGGCC CTGCAAAAAG 
CGTGGAAGGA AAGAAAAGGT GACCGAGGAG CCGCTGCACT CAATTCAGCA GTCTGCAAGT 
TCTGAGGCCC AAACAGATGA AGGGTATGCT TCCCAAACCA TGCCATCACC AGTGTTCAGC 
CCCAGCTTCG AAGTGCCACA GGCGTATTGC AGATCTCCTT TCGAAGACAA GTACAAATCA 
GGGATGAGCA AAATGAAGCA CGAGTCGGAT CTCAGCCACC TGAGATACGA GATGCCAGCA 
AGTCCCTTCC GATCTCACTC TCAGCAGGCC TTCCACAGCA CCAGGGACCT CACCATGGTG 
CAGTCTCCTT CCAGCGGAAT GGTCGGAAAC AAGGTCAAGA ATGAGGCTGT TCCTCAAATG 
TCTTGCCAGT TTGCTGCGGC TCCGAGCGAG AACCTTGGCT TGCAGAAGCA GGACTATGGC 
AGTGCTGAAT CTCAGGGTCC TTGTTTAGGA GCTTCCGGGA GGGAGGACAC AAAGGTAGTA 
GTCAAAAACG AGTATGGCGG CGAGTCCTGC ATCCGGAACA CTGCGGGTAA AGAGGCGGAA 
ATGTTCAGCA AACATCTCGG ACAGTTACAA TCACCGACAC TTCAGAGTCC TCTAGAAAAA 
CCTCATCATC ATGCGCGAAA AAGCATACAG AGTTCACCCA GCCCCATGAT TCAGCCGTCC 
TATCTTCCAC CTCAGCAAGA CCTGAGTTAC AGCAACCATT ACTTCAGCGG CAGCTACATG 
CATCAGGGCA GGCAGCCTAG CCCCGGCCTG TGCACTCTTT CGCAAAATCC GCTCGTGTCT 
GGCATTCCGG AGAGTCCCCC TGAAGACCAT CACCTCGCAA TGAACTGCTA CAGGATGACC 
GACAGGTACC ATGCCTACCC AAACCCTCTT CAGCACGTCG GTCAGGAAGG GCACGCTCTG 
GAAACTCACG GGATAGACCA CCTCCAGAAC TTGGCGTCCG TCTCAAACCA TCACCTGCAG 
AACCTGGAGG TCAACCGGCC TAGCGGGGGT CACGGCGTGT ACCCGCCCTT CATGGGGAGG 
CCTGCGCCGA TCCAGCACTA CGACCATCAA CCCATGAACA ACCCCACGTT CAACCACATC 
TCCCAGGCGC CTTCACCGCA GGGGGTCATG CTTCCACCCC AGTCTTACAT TGCCCAGCCC 
GTCTCGCCGC ACCTGACGAG GAACGTCGGC TACGGCGTGC CCGGGCTCTC CAGGACCGGC 
GGCTTCCTCC CGTCTCCTCA CGAGGCCTTC TTATCTCACC CCAGTCCTCC CGTCACCCCC 
ATGACTCCTC CCAGCACACC GAGGCTCACG GACCTCAATC CCGTCAGGAT CGACTCTCCG 
CTCCACTTGC GAGCCTCTTA CTACTCGGCC GCGACGAGTC CCTTCGGCGT GCCGGTCGAC 
CCGTCCTACT CTCCGCTGGC CAGGAGCCAC AGTCAGGGGC CCGCCTTTGC GCCATACATG 
GACGGAGCGG CCTGGGCCTC CGACCGTGAC TACAACAGTG CGGCTCTCCA AAACTCCTTT 
TACGTGCCGC GCTCCCAGTG CTTCCCGACG GCAGCACCGC CGCTGCCCCA GAAAGACTAT 
GCGTTCCGCG CGCCCCCGGG CAGCCACTTT GCAGGACCTT CTCTTCCCTC TTCGTCGCGT 
CTTCCGCCCG GCCTGGCCAC GCCTCCGCAG CAGGACCGCT CTGCCACCGC CAGCGTGCTT 
CCCCCGTTCA AGAACACGTT TCTCCCGGCG CAGTCTGCCC TCAACGGTTT CGAGCGTCAC 
CTGCAGACGT TCAAGTCGGT GATGATGGAA GACGCCGCGC AGAGGAGCTC CGCCTCTGGG 
CTCTACGGCG CTCCGGCCCC CACGGGCCGC CCTTCTCAGC GGCCAGCTAG TCACGAGGGC 
TTCACGAGGA CGCCTCTCGA ACCGCCCGTC ACGGAAGAGT CTTCCGAGGG GATCTACGAG 
GTGGACTCGG ACAACGAAAG CGCCTTCCAG GACGAAGAGG TTGGCGGCGT CGCCATTGCG 
CTCACTCACG GGTCGGTGCT CTTCGAGTGC GCCAAGCACG AGCTGCATGC GACGACTGCT 
CTGCGGAGGC CCAACCGCAA GAACCCCACC CGCATCAGCC TCGTGTTCTA CCAGCACAAG 
AACCTCAACT TCCGGAATCA CGGGGAGGAG GAGTGGGAGA AGAAGATGGA GGTTCGCAAG 
TTGGAAAGAG CCAACGGGGA GCGGCCCTCC AAGAAGAAGG CCAAGCTCTC CGTGGACAGG 
TTGGATGAGG GTGGCGGCTT CCCCGCGGCT CCCGCAGACG TCGGCCAGCC GTGGGAGGTG 
CCCCCCCGCG TGTCCCTGCC GTCGTTCTTC GCCATCAACC CTTACCAGAA GTGTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838411.1
CDS	102..5861
Translation	MPTPVDKMAAQHPASQFGSPMQHHPFADFPPHHAVFFAPDKGNAEEPSFQVPSVSSTTTRHTLLPMVPLQEPWGDQPPMARNGLVMHHQQPQQQPPHHHHQGGPPQQQQQQQQQPGTPDGMEKGTKKKRKRCGECPGCLKKDNCGECGPCRSVRSHQICKMRKCDQLKTKKEKVASAICGSGVAGKDGMAASPGNRTNLNGRAVNGGGASTSPYLPQGVPEHLGHPGIGGFPGHSEYQLPPTQKPAPPAQAGQMYEMGPHFGAFPGHPSQALAFNGHMAQHDAGRLGLPGEEGDFPTRHQMMNSRLKTLIQTRQSQKEQGPFQGLETTLGPSVGGSFVHPQADATATASRGFPPLVRSNGGCGAVSGNNNGGNNGGGNNNNNGTNNGGGNNGNGGTGGGNNNPRPLSSNEMPGDAWHDASPSSGPPPGRAGVAELPEFAKNGSSSSSQGSNNNANNNNSEAEFSQPEDQSHESEFAEGGAAETRGGGGGDRSSAPPNGMLPYGSFYGAQEAAEVRKGGEGKVPPPTTSAATTTSAADVHGGGAHYQFTSAATSMSTTTTSASVHGKPCNGPTQPSFAERQSPRGGPFSGAPTSFAPAPHLASMHQERPVPESPTQLRNEVGEAGSGGQTRGNESESTDAAAVTSQPFLSNTTTSMNTYTSIINTYNSFAASCAPSFLAEHQQPKFGGSVEDAARTGGGDASDVDGGRIAVGEFRGLDFGAPPLPHHPGGPEGPRPHYPQYGGAPAAFVPEGPYAVPYGAGNLGNLGNPFPLPPMGAGGPPFAPMSLPGADPWWERLERLRSNAKAEPPACDCYGADETPPVDKSPYYTHLGAGPTVAAIREMLEHRLNERGPALRIEKVLYSGKEGKTSQGCPVAKWIIRRSGPSEKVLAVLRHRPGHRCLSAYIVMAIVAWEGVQADMADDLYRTVTHKTVNFGFPTQRRCGTNEQRTCACQGADAENCGASFSFGCSWSMYYNGCKYARSKAVRKFKLSEQSQEQELEDKMQMLATEMAPLYARVAPESYKNQTEFENEGISCRLGLKQGRPFSGVTACVDFCAHAHKDLHNMNNGCTVVVTLTRHRGFDKGDDEQLHVLPLYILDATDEQGGKEGFHEKIKNGSLEVLSKFHTTSRIRKVPLGPCKKRGRKEKVTEEPLHSIQQSASSEAQTDEGYASQTMPSPVFSPSFEVPQAYCRSPFEDKYKSGMSKMKHESDLSHLRYEMPASPFRSHSQQAFHSTRDLTMVQSPSSGMVGNKVKNEAVPQMSCQFAAAPSENLGLQKQDYGSAESQGPCLGASGREDTKVVVKNEYGGESCIRNTAGKEAEMFSKHLGQLQSPTLQSPLEKPHHHARKSIQSSPSPMIQPSYLPPQQDLSYSNHYFSGSYMHQGRQPSPGLCTLSQNPLVSGIPESPPEDHHLAMNCYRMTDRYHAYPNPLQHVGQEGHALETHGIDHLQNLASVSNHHLQNLEVNRPSGGHGVYPPFMGRPAPIQHYDHQPMNNPTFNHISQAPSPQGVMLPPQSYIAQPVSPHLTRNVGYGVPGLSRTGGFLPSPHEAFLSHPSPPVTPMTPPSTPRLTDLNPVRIDSPLHLRASYYSAATSPFGVPVDPSYSPLARSHSQGPAFAPYMDGAAWASDRDYNSAALQNSFYVPRSQCFPTAAPPLPQKDYAFRAPPGSHFAGPSLPSSSRLPPGLATPPQQDRSATASVLPPFKNTFLPAQSALNGFERHLQTFKSVMMEDAAQRSSASGLYGAPAPTGRPSQRPASHEGFTRTPLEPPVTEESSEGIYEVDSDNESAFQDEEVGGVAIALTHGSVLFECAKHELHATTALRRPNRKNPTRISLVFYQHKNLNFRNHGEEEWEKKMEVRKLERANGERPSKKKAKLSVDRLDEGGGFPAAPADVGQPWEVPPRVSLPSFFAINPYQKCF

Target ORF information:

RefSeq Version	XM_029982551.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982551.1

ORF Insert Sequence:

ATGCCCACCC CCGTAGACAA GATGGCGGCG CAGCACCCGG CGTCCCAGTT CGGCAGTCCG 
ATGCAGCATC ATCCCTTCGC CGACTTCCCC CCGCACCACG CGGTGTTCTT TGCGCCCGAC 
AAGGGCAACG CCGAAGAGCC GTCCTTCCAG GTGCCCTCCG TGTCGTCCAC CACGACTAGG 
CACACGCTGC TGCCCATGGT GCCGCTCCAG GAGCCCTGGG GAGACCAGCC GCCCATGGCC 
AGGAACGGCC TGGTGATGCA CCACCAGCAG CCGCAGCAGC AGCCGCCTCA CCACCACCAC 
CAGGGCGGGC CGCCCCAGCA GCAGCAGCAA CAGCAGCAGC AGCCCGGCAC CCCGGACGGC 
ATGGAGAAGG GCACCAAGAA GAAACGCAAA CGCTGCGGCG AGTGCCCTGG TTGCCTCAAG 
AAGGATAACT GCGGAGAGTG CGGGCCGTGC AGGAGTGTGC GCAGTCACCA GATCTGCAAG 
ATGCGGAAGT GCGATCAGCT CAAGACCAAA AAGGAGAAAG TAGCGAGCGC CATTTGCGGA 
AGCGGGGTCG CCGGAAAAGA CGGCATGGCG GCCTCTCCGG GCAACCGAAC TAATCTCAAC 
GGGCGGGCGG TGAACGGCGG CGGCGCTTCG ACGTCGCCCT ACCTCCCGCA GGGAGTGCCG 
GAACACCTCG GACATCCTGG GATCGGTGGC TTTCCCGGTC ACAGCGAGTA CCAGCTCCCG 
CCCACGCAAA AGCCTGCCCC GCCGGCCCAG GCCGGCCAGA TGTACGAGAT GGGGCCGCAC 
TTCGGGGCCT TCCCGGGCCA CCCGTCTCAG GCCCTGGCTT TCAATGGCCA CATGGCACAG 
CACGACGCTG GCCGCCTGGG GCTCCCCGGC GAAGAGGGCG ACTTCCCCAC GCGCCACCAG 
ATGATGAACA GCCGTCTCAA GACACTGATT CAGACTAGGC AGAGCCAGAA GGAGCAGGGT 
CCCTTCCAGG GGCTCGAGAC GACCCTAGGC CCCTCCGTCG GAGGAAGCTT TGTGCACCCG 
CAGGCCGATG CGACGGCGAC TGCCTCCCGG GGGTTCCCGC CGCTTGTGCG GAGCAACGGT 
GGCTGCGGTG CCGTGAGCGG CAACAACAAC GGCGGAAACA ATGGCGGCGG CAACAACAAC 
AACAACGGCA CCAACAACGG CGGTGGTAAC AACGGCAACG GCGGCACGGG CGGCGGTAAC 
AACAATCCGA GGCCTCTGTC GTCAAACGAG ATGCCCGGGG ATGCGTGGCA CGATGCGAGC 
CCGTCCTCGG GTCCTCCGCC GGGCAGGGCT GGTGTCGCAG AGCTACCGGA GTTTGCCAAG 
AACGGGTCCT CTTCCTCCTC CCAGGGAAGC AACAACAACG CCAACAACAA CAACTCCGAA 
GCTGAGTTCT CCCAGCCTGA GGATCAGAGT CACGAGAGTG AGTTTGCAGA GGGTGGCGCT 
GCGGAAACCA GGGGAGGTGG CGGCGGCGAC AGGTCGAGCG CTCCCCCCAA CGGAATGTTG 
CCCTACGGAA GCTTCTATGG GGCGCAAGAG GCGGCAGAAG TCCGGAAGGG TGGCGAGGGC 
AAGGTCCCCC CGCCGACAAC GAGCGCGGCG ACGACCACTT CGGCGGCGGA CGTTCACGGT 
GGTGGTGCGC ATTACCAGTT CACGTCGGCA GCGACTTCGA TGAGCACGAC GACGACGAGT 
GCCTCCGTTC ACGGCAAACC CTGCAACGGA CCAACGCAGC CTTCGTTTGC GGAGCGGCAG 
TCGCCCAGGG GCGGCCCGTT CTCCGGTGCA CCGACGTCAT TCGCTCCGGC GCCCCACTTG 
GCGAGTATGC ACCAAGAGCG CCCGGTCCCC GAAAGTCCGA CGCAGCTCCG CAACGAGGTA 
GGCGAAGCCG GCAGCGGCGG CCAGACGCGC GGCAATGAGA GCGAATCCAC GGACGCGGCG 
GCAGTCACAT CGCAGCCTTT TTTATCGAAT ACGACCACAT CTATGAATAC ATATACAAGT 
ATTATCAATA CATATAACAG CTTCGCCGCT TCGTGCGCGC CGTCCTTCTT GGCCGAGCAC 
CAGCAACCCA AGTTTGGCGG GAGCGTGGAG GATGCGGCTC GAACGGGGGG AGGAGACGCA 
TCCGACGTAG ATGGTGGTCG TATTGCCGTC GGCGAATTCC GTGGACTCGA CTTTGGGGCA 
CCCCCCCTGC CTCACCACCC CGGTGGCCCC GAGGGCCCCA GGCCGCACTA CCCCCAGTAC 
GGGGGGGCGC CTGCCGCGTT TGTGCCAGAG GGTCCTTACG CTGTTCCCTA TGGGGCCGGG 
AACCTGGGGA ATCTGGGGAA CCCGTTCCCC TTGCCCCCCA TGGGCGCCGG AGGTCCTCCC 
TTTGCGCCCA TGAGCCTCCC TGGTGCCGAC CCCTGGTGGG AGCGGCTGGA GCGGCTACGC 
AGCAACGCCA AGGCGGAGCC TCCGGCCTGC GACTGCTATG GCGCGGATGA GACGCCTCCC 
GTGGATAAAT CCCCATACTA TACCCACCTT GGTGCTGGAC CGACTGTGGC TGCTATTAGA 
GAGATGCTTG AGCACAGGCT GAACGAACGG GGCCCTGCGT TGCGCATCGA GAAGGTGCTC 
TACTCGGGCA AGGAGGGCAA GACGAGCCAG GGCTGCCCGG TGGCCAAGTG GATCATCCGG 
CGCAGTGGCC CTTCGGAGAA GGTGCTGGCC GTGCTGCGGC ACCGACCGGG CCACCGCTGC 
TTGTCGGCCT ACATCGTGAT GGCCATCGTC GCCTGGGAGG GCGTCCAGGC AGACATGGCA 
GACGACCTCT ACAGGACCGT GACCCACAAG ACCGTCAACT TTGGCTTCCC CACTCAGCGG 
AGATGTGGCA CCAACGAACA ACGAACCTGC GCCTGTCAAG GTGCCGACGC CGAGAACTGT 
GGCGCTTCCT TTTCATTTGG CTGCTCTTGG AGCATGTATT ACAATGGCTG CAAGTATGCA 
CGGAGCAAGG CTGTCCGCAA GTTCAAGCTC TCAGAACAGT CCCAGGAGCA AGAGCTTGAA 
GACAAAATGC AGATGCTCGC CACTGAAATG GCACCGCTGT ATGCAAGGGT GGCTCCGGAA 
TCCTACAAAA ACCAGACCGA GTTTGAGAAC GAAGGGATAT CCTGCAGACT AGGGTTGAAG 
CAGGGAAGAC CCTTTTCTGG CGTCACGGCG TGTGTCGACT TCTGTGCCCA TGCGCACAAG 
GACCTACACA ACATGAATAA CGGCTGTACA GTTGTTGTAA CCCTAACGAG ACATCGAGGA 
TTTGACAAGG GCGACGACGA ACAGCTGCAC GTGCTTCCAC TCTACATACT GGACGCAACC 
GACGAGCAGG GTGGCAAAGA AGGCTTTCAT GAAAAGATCA AGAATGGGTC ACTCGAGGTT 
TTGTCGAAAT TTCACACGAC GTCCAGGATA AGAAAGGTCC CCTTGGGGCC CTGCAAAAAG 
CGTGGAAGGA AAGAAAAGGT GACCGAGGAG CCGCTGCACT CAATTCAGCA GTCTGCAAGT 
TCTGAGGCCC AAACAGATGA AGGGTATGCT TCCCAAACCA TGCCATCACC AGTGTTCAGC 
CCCAGCTTCG AAGTGCCACA GGCGTATTGC AGATCTCCTT TCGAAGACAA GTACAAATCA 
GGGATGAGCA AAATGAAGCA CGAGTCGGAT CTCAGCCACC TGAGATACGA GATGCCAGCA 
AGTCCCTTCC GATCTCACTC TCAGCAGGCC TTCCACAGCA CCAGGGACCT CACCATGGTG 
CAGTCTCCTT CCAGCGGAAT GGTCGGAAAC AAGGTCAAGA ATGAGGCTGT TCCTCAAATG 
TCTTGCCAGT TTGCTGCGGC TCCGAGCGAG AACCTTGGCT TGCAGAAGCA GGACTATGGC 
AGTGCTGAAT CTCAGGGTCC TTGTTTAGGA GCTTCCGGGA GGGAGGACAC AAAGGTAGTA 
GTCAAAAACG AGTATGGCGG CGAGTCCTGC ATCCGGAACA CTGCGGGTAA AGAGGCGGAA 
ATGTTCAGCA AACATCTCGG ACAGTTACAA TCACCGACAC TTCAGAGTCC TCTAGAAAAA 
CCTCATCATC ATGCGCGAAA AAGCATACAG AGTTCACCCA GCCCCATGAT TCAGCCGTCC 
TATCTTCCAC CTCAGCAAGA CCTGAGTTAC AGCAACCATT ACTTCAGCGG CAGCTACATG 
CATCAGGGCA GGCAGCCTAG CCCCGGCCTG TGCACTCTTT CGCAAAATCC GCTCGTGTCT 
GGCATTCCGG AGAGTCCCCC TGAAGACCAT CACCTCGCAA TGAACTGCTA CAGGATGACC 
GACAGGTACC ATGCCTACCC AAACCCTCTT CAGCACGTCG GTCAGGAAGG GCACGCTCTG 
GAAACTCACG GGATAGACCA CCTCCAGAAC TTGGCGTCCG TCTCAAACCA TCACCTGCAG 
AACCTGGAGG TCAACCGGCC TAGCGGGGGT CACGGCGTGT ACCCGCCCTT CATGGGGAGG 
CCTGCGCCGA TCCAGCACTA CGACCATCAA CCCATGAACA ACCCCACGTT CAACCACATC 
TCCCAGGCGC CTTCACCGCA GGGGGTCATG CTTCCACCCC AGTCTTACAT TGCCCAGCCC 
GTCTCGCCGC ACCTGACGAG GAACGTCGGC TACGGCGTGC CCGGGCTCTC CAGGACCGGC 
GGCTTCCTCC CGTCTCCTCA CGAGGCCTTC TTATCTCACC CCAGTCCTCC CGTCACCCCC 
ATGACTCCTC CCAGCACACC GAGGCTCACG GACCTCAATC CCGTCAGGAT CGACTCTCCG 
CTCCACTTGC GAGCCTCTTA CTACTCGGCC GCGACGAGTC CCTTCGGCGT GCCGGTCGAC 
CCGTCCTACT CTCCGCTGGC CAGGAGCCAC AGTCAGGGGC CCGCCTTTGC GCCATACATG 
GACGGAGCGG CCTGGGCCTC CGACCGTGAC TACAACAGTG CGGCTCTCCA AAACTCCTTT 
TACGTGCCGC GCTCCCAGTG CTTCCCGACG GCAGCACCGC CGCTGCCCCA GAAAGACTAT 
GCGTTCCGCG CGCCCCCGGG CAGCCACTTT GCAGGACCTT CTCTTCCCTC TTCGTCGCGT 
CTTCCGCCCG GCCTGGCCAC GCCTCCGCAG CAGGACCGCT CTGCCACCGC CAGCGTGCTT 
CCCCCGTTCA AGAACACGTT TCTCCCGGCG CAGTCTGCCC TCAACGGTTT CGAGCGTCAC 
CTGCAGACGT TCAAGTCGGT GATGATGGAA GACGCCGCGC AGAGGAGCTC CGCCTCTGGG 
CTCTACGGCG CTCCGGCCCC CACGGGCCGC CCTTCTCAGC GGCCAGCTAG TCACGAGGGC 
TTCACGAGGA CGCCTCTCGA ACCGCCCGTC ACGGAAGAGT CTTCCGAGGG GATCTACGAG 
GTGGACTCGG ACAACGAAAG CGCCTTCCAG GACGAAGAGG TTGGCGGCGT CGCCATTGCG 
CTCACTCACG GGTCGGTGCT CTTCGAGTGC GCCAAGCACG AGCTGCATGC GACGACTGCT 
CTGCGGAGGC CCAACCGCAA GAACCCCACC CGCATCAGCC TCGTGTTCTA CCAGCACAAG 
AACCTCAACT TCCGGAATCA CGGGGAGGAG GAGTGGGAGA AGAAGATGGA GGTTCGCAAG 
TTGGAAAGAG CCAACGGGGA GCGGCCCTCC AAGAAGAAGG CCAAGCTCTC CGTGGACAGG 
TTGGATGAGG GTGGCGGCTT CCCCGCGGCT CCCGCAGACG TCGGCCAGCC GTGGGAGGTG 
CCCCCCCGCG TGTCCCTGCC GTCGTTCTTC GCCATCAACC CTTACCAGAA GTGTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8042599 ( XM_029982552.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838412.1 Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X3, mRNA.

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CloneID	OIa25174
Clone ID Related Accession (Same CDS sequence)	XM_029982552.1
Accession Version	XM_029982552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8042599 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919951.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACCC CCGTAGACAA GATGGCGGCG CAGCACCCGG CGTCCCAGTT CGGCAGTCCG 
ATGCAGCATC ATCCCTTCGC CGACTTCCCC CCGCACCACG CGGTGTTCTT TGCGCCCGAC 
AAGGGCAACG CCGAAGAGCC GTCCTTCCAG GTGCCCTCCG TGTCGTCCAC CACGACTAGG 
CACACGCTGC TGCCCATGGT GCCGCTCCAG GAGCCCTGGG GAGACCAGCC GCCCATGGCC 
AGGAACGGCC TGGTGATGCA CCACCAGCAG CCGCAGCAGC AGCCGCCTCA CCACCACCAC 
CAGGGCGGGC CGCCCCAGCA GCAGCAGCAA CAGCAGCAGC AGCCCGGCAC CCCGGACGGC 
ATGGAGAAGG GCACCAAGAA GAAACGCAAA CGCTGCGGCG AGTGCCCTGG TTGCCTCAAG 
AAGGATAACT GCGGAGAGTG CGGGCCGTGC AGGAGTGTGC GCAGTCACCA GATCTGCAAG 
ATGCGGAAGT GCGATCAGCT CAAGACCAAA AAGGAGAAAG AGCGCTATGA GAAGATGATG 
CATCAGATGC ACGAAAGGCG TCGGGGTACA CCAGATGAAA ATAAAAACTT GCAACAGATC 
GGTGGCTTTC CCGGTCACAG CGAGTACCAG CTCCCGCCCA CGCAAAAGCC TGCCCCGCCG 
GCCCAGGCCG GCCAGATGTA CGAGATGGGG CCGCACTTCG GGGCCTTCCC GGGCCACCCG 
TCTCAGGCCC TGGCTTTCAA TGGCCACATG GCACAGCACG ACGCTGGCCG CCTGGGGCTC 
CCCGGCGAAG AGGGCGACTT CCCCACGCGC CACCAGATGA TGAACAGCCG TCTCAAGACA 
CTGATTCAGA CTAGGCAGAG CCAGAAGGAG CAGGGTCCCT TCCAGGGGCT CGAGACGACC 
CTAGGCCCCT CCGTCGGAGG AAGCTTTGTG CACCCGCAGG CCGATGCGAC GGCGACTGCC 
TCCCGGGGGT TCCCGCCGCT TGTGCGGAGC AACGGTGGCT GCGGTGCCGT GAGCGGCAAC 
AACAACGGCG GAAACAATGG CGGCGGCAAC AACAACAACA ACGGCACCAA CAACGGCGGT 
GGTAACAACG GCAACGGCGG CACGGGCGGC GGTAACAACA ATCCGAGGCC TCTGTCGTCA 
AACGAGATGC CCGGGGATGC GTGGCACGAT GCGAGCCCGT CCTCGGGTCC TCCGCCGGGC 
AGGGCTGGTG TCGCAGAGCT ACCGGAGTTT GCCAAGAACG GGTCCTCTTC CTCCTCCCAG 
GGAAGCAACA ACAACGCCAA CAACAACAAC TCCGAAGCTG AGTTCTCCCA GCCTGAGGAT 
CAGAGTCACG AGAGTGAGTT TGCAGAGGGT GGCGCTGCGG AAACCAGGGG AGGTGGCGGC 
GGCGACAGGT CGAGCGCTCC CCCCAACGGA ATGTTGCCCT ACGGAAGCTT CTATGGGGCG 
CAAGAGGCGG CAGAAGTCCG GAAGGGTGGC GAGGGCAAGG TCCCCCCGCC GACAACGAGC 
GCGGCGACGA CCACTTCGGC GGCGGACGTT CACGGTGGTG GTGCGCATTA CCAGTTCACG 
TCGGCAGCGA CTTCGATGAG CACGACGACG ACGAGTGCCT CCGTTCACGG CAAACCCTGC 
AACGGACCAA CGCAGCCTTC GTTTGCGGAG CGGCAGTCGC CCAGGGGCGG CCCGTTCTCC 
GGTGCACCGA CGTCATTCGC TCCGGCGCCC CACTTGGCGA GTATGCACCA AGAGCGCCCG 
GTCCCCGAAA GTCCGACGCA GCTCCGCAAC GAGGTAGGCG AAGCCGGCAG CGGCGGCCAG 
ACGCGCGGCA ATGAGAGCGA ATCCACGGAC GCGGCGGCAG TCACATCGCA GCCTTTTTTA 
TCGAATACGA CCACATCTAT GAATACATAT ACAAGTATTA TCAATACATA TAACAGCTTC 
GCCGCTTCGT GCGCGCCGTC CTTCTTGGCC GAGCACCAGC AACCCAAGTT TGGCGGGAGC 
GTGGAGGATG CGGCTCGAAC GGGGGGAGGA GACGCATCCG ACGTAGATGG TGGTCGTATT 
GCCGTCGGCG AATTCCGTGG ACTCGACTTT GGGGCACCCC CCCTGCCTCA CCACCCCGGT 
GGCCCCGAGG GCCCCAGGCC GCACTACCCC CAGTACGGGG GGGCGCCTGC CGCGTTTGTG 
CCAGAGGGTC CTTACGCTGT TCCCTATGGG GCCGGGAACC TGGGGAATCT GGGGAACCCG 
TTCCCCTTGC CCCCCATGGG CGCCGGAGGT CCTCCCTTTG CGCCCATGAG CCTCCCTGGT 
GCCGACCCCT GGTGGGAGCG GCTGGAGCGG CTACGCAGCA ACGCCAAGGC GGAGCCTCCG 
GCCTGCGACT GCTATGGCGC GGATGAGACG CCTCCCGTGG ATAAATCCCC ATACTATACC 
CACCTTGGTG CTGGACCGAC TGTGGCTGCT ATTAGAGAGA TGCTTGAGCA CAGGCTGAAC 
GAACGGGGCC CTGCGTTGCG CATCGAGAAG GTGCTCTACT CGGGCAAGGA GGGCAAGACG 
AGCCAGGGCT GCCCGGTGGC CAAGTGGATC ATCCGGCGCA GTGGCCCTTC GGAGAAGGTG 
CTGGCCGTGC TGCGGCACCG ACCGGGCCAC CGCTGCTTGT CGGCCTACAT CGTGATGGCC 
ATCGTCGCCT GGGAGGGCGT CCAGGCAGAC ATGGCAGACG ACCTCTACAG GACCGTGACC 
CACAAGACCG TCAACTTTGG CTTCCCCACT CAGCGGAGAT GTGGCACCAA CGAACAACGA 
ACCTGCGCCT GTCAAGGTGC CGACGCCGAG AACTGTGGCG CTTCCTTTTC ATTTGGCTGC 
TCTTGGAGCA TGTATTACAA TGGCTGCAAG TATGCACGGA GCAAGGCTGT CCGCAAGTTC 
AAGCTCTCAG AACAGTCCCA GGAGCAAGAG CTTGAAGACA AAATGCAGAT GCTCGCCACT 
GAAATGGCAC CGCTGTATGC AAGGGTGGCT CCGGAATCCT ACAAAAACCA GACCGAGTTT 
GAGAACGAAG GGATATCCTG CAGACTAGGG TTGAAGCAGG GAAGACCCTT TTCTGGCGTC 
ACGGCGTGTG TCGACTTCTG TGCCCATGCG CACAAGGACC TACACAACAT GAATAACGGC 
TGTACAGTTG TTGTAACCCT AACGAGACAT CGAGGATTTG ACAAGGGCGA CGACGAACAG 
CTGCACGTGC TTCCACTCTA CATACTGGAC GCAACCGACG AGCAGGGTGG CAAAGAAGGC 
TTTCATGAAA AGATCAAGAA TGGGTCACTC GAGGTTTTGT CGAAATTTCA CACGACGTCC 
AGGATAAGAA AGGTCCCCTT GGGGCCCTGC AAAAAGCGTG GAAGGAAAGA AAAGGTGACC 
GAGGAGCCGC TGCACTCAAT TCAGCAGTCT GCAAGTTCTG AGGCCCAAAC AGATGAAGGG 
TATGCTTCCC AAACCATGCC ATCACCAGTG TTCAGCCCCA GCTTCGAAGT GCCACAGGCG 
TATTGCAGAT CTCCTTTCGA AGACAAGTAC AAATCAGGGA TGAGCAAAAT GAAGCACGAG 
TCGGATCTCA GCCACCTGAG ATACGAGATG CCAGCAAGTC CCTTCCGATC TCACTCTCAG 
CAGGCCTTCC ACAGCACCAG GGACCTCACC ATGGTGCAGT CTCCTTCCAG CGGAATGGTC 
GGAAACAAGG TCAAGAATGA GGCTGTTCCT CAAATGTCTT GCCAGTTTGC TGCGGCTCCG 
AGCGAGAACC TTGGCTTGCA GAAGCAGGAC TATGGCAGTG CTGAATCTCA GGGTCCTTGT 
TTAGGAGCTT CCGGGAGGGA GGACACAAAG GTAGTAGTCA AAAACGAGTA TGGCGGCGAG 
TCCTGCATCC GGAACACTGC GGGTAAAGAG GCGGAAATGT TCAGCAAACA TCTCGGACAG 
TTACAATCAC CGACACTTCA GAGTCCTCTA GAAAAACCTC ATCATCATGC GCGAAAAAGC 
ATACAGAGTT CACCCAGCCC CATGATTCAG CCGTCCTATC TTCCACCTCA GCAAGACCTG 
AGTTACAGCA ACCATTACTT CAGCGGCAGC TACATGCATC AGGGCAGGCA GCCTAGCCCC 
GGCCTGTGCA CTCTTTCGCA AAATCCGCTC GTGTCTGGCA TTCCGGAGAG TCCCCCTGAA 
GACCATCACC TCGCAATGAA CTGCTACAGG ATGACCGACA GGTACCATGC CTACCCAAAC 
CCTCTTCAGC ACGTCGGTCA GGAAGGGCAC GCTCTGGAAA CTCACGGGAT AGACCACCTC 
CAGAACTTGG CGTCCGTCTC AAACCATCAC CTGCAGAACC TGGAGGTCAA CCGGCCTAGC 
GGGGGTCACG GCGTGTACCC GCCCTTCATG GGGAGGCCTG CGCCGATCCA GCACTACGAC 
CATCAACCCA TGAACAACCC CACGTTCAAC CACATCTCCC AGGCGCCTTC ACCGCAGGGG 
GTCATGCTTC CACCCCAGTC TTACATTGCC CAGCCCGTCT CGCCGCACCT GACGAGGAAC 
GTCGGCTACG GCGTGCCCGG GCTCTCCAGG ACCGGCGGCT TCCTCCCGTC TCCTCACGAG 
GCCTTCTTAT CTCACCCCAG TCCTCCCGTC ACCCCCATGA CTCCTCCCAG CACACCGAGG 
CTCACGGACC TCAATCCCGT CAGGATCGAC TCTCCGCTCC ACTTGCGAGC CTCTTACTAC 
TCGGCCGCGA CGAGTCCCTT CGGCGTGCCG GTCGACCCGT CCTACTCTCC GCTGGCCAGG 
AGCCACAGTC AGGGGCCCGC CTTTGCGCCA TACATGGACG GAGCGGCCTG GGCCTCCGAC 
CGTGACTACA ACAGTGCGGC TCTCCAAAAC TCCTTTTACG TGCCGCGCTC CCAGTGCTTC 
CCGACGGCAG CACCGCCGCT GCCCCAGAAA GACTATGCGT TCCGCGCGCC CCCGGGCAGC 
CACTTTGCAG GACCTTCTCT TCCCTCTTCG TCGCGTCTTC CGCCCGGCCT GGCCACGCCT 
CCGCAGCAGG ACCGCTCTGC CACCGCCAGC GTGCTTCCCC CGTTCAAGAA CACGTTTCTC 
CCGGCGCAGT CTGCCCTCAA CGGTTTCGAG CGTCACCTGC AGACGTTCAA GTCGGTGATG 
ATGGAAGACG CCGCGCAGAG GAGCTCCGCC TCTGGGCTCT ACGGCGCTCC GGCCCCCACG 
GGCCGCCCTT CTCAGCGGCC AGCTAGTCAC GAGGGCTTCA CGAGGACGCC TCTCGAACCG 
CCCGTCACGG AAGAGTCTTC CGAGGGGATC TACGAGGTGG ACTCGGACAA CGAAAGCGCC 
TTCCAGGACG AAGAGGTTGG CGGCGTCGCC ATTGCGCTCA CTCACGGGTC GGTGCTCTTC 
GAGTGCGCCA AGCACGAGCT GCATGCGACG ACTGCTCTGC GGAGGCCCAA CCGCAAGAAC 
CCCACCCGCA TCAGCCTCGT GTTCTACCAG CACAAGAACC TCAACTTCCG GAATCACGGG 
GAGGAGGAGT GGGAGAAGAA GATGGAGGTT CGCAAGTTGG AAAGAGCCAA CGGGGAGCGG 
CCCTCCAAGA AGAAGGCCAA GCTCTCCGTG GACAGGTTGG ATGAGGGTGG CGGCTTCCCC 
GCGGCTCCCG CAGACGTCGG CCAGCCGTGG GAGGTGCCCC CCCGCGTGTC CCTGCCGTCG 
TTCTTCGCCA TCAACCCTTA CCAGAAGTGT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838412.1
CDS	102..5777
Translation	MPTPVDKMAAQHPASQFGSPMQHHPFADFPPHHAVFFAPDKGNAEEPSFQVPSVSSTTTRHTLLPMVPLQEPWGDQPPMARNGLVMHHQQPQQQPPHHHHQGGPPQQQQQQQQQPGTPDGMEKGTKKKRKRCGECPGCLKKDNCGECGPCRSVRSHQICKMRKCDQLKTKKEKERYEKMMHQMHERRRGTPDENKNLQQIGGFPGHSEYQLPPTQKPAPPAQAGQMYEMGPHFGAFPGHPSQALAFNGHMAQHDAGRLGLPGEEGDFPTRHQMMNSRLKTLIQTRQSQKEQGPFQGLETTLGPSVGGSFVHPQADATATASRGFPPLVRSNGGCGAVSGNNNGGNNGGGNNNNNGTNNGGGNNGNGGTGGGNNNPRPLSSNEMPGDAWHDASPSSGPPPGRAGVAELPEFAKNGSSSSSQGSNNNANNNNSEAEFSQPEDQSHESEFAEGGAAETRGGGGGDRSSAPPNGMLPYGSFYGAQEAAEVRKGGEGKVPPPTTSAATTTSAADVHGGGAHYQFTSAATSMSTTTTSASVHGKPCNGPTQPSFAERQSPRGGPFSGAPTSFAPAPHLASMHQERPVPESPTQLRNEVGEAGSGGQTRGNESESTDAAAVTSQPFLSNTTTSMNTYTSIINTYNSFAASCAPSFLAEHQQPKFGGSVEDAARTGGGDASDVDGGRIAVGEFRGLDFGAPPLPHHPGGPEGPRPHYPQYGGAPAAFVPEGPYAVPYGAGNLGNLGNPFPLPPMGAGGPPFAPMSLPGADPWWERLERLRSNAKAEPPACDCYGADETPPVDKSPYYTHLGAGPTVAAIREMLEHRLNERGPALRIEKVLYSGKEGKTSQGCPVAKWIIRRSGPSEKVLAVLRHRPGHRCLSAYIVMAIVAWEGVQADMADDLYRTVTHKTVNFGFPTQRRCGTNEQRTCACQGADAENCGASFSFGCSWSMYYNGCKYARSKAVRKFKLSEQSQEQELEDKMQMLATEMAPLYARVAPESYKNQTEFENEGISCRLGLKQGRPFSGVTACVDFCAHAHKDLHNMNNGCTVVVTLTRHRGFDKGDDEQLHVLPLYILDATDEQGGKEGFHEKIKNGSLEVLSKFHTTSRIRKVPLGPCKKRGRKEKVTEEPLHSIQQSASSEAQTDEGYASQTMPSPVFSPSFEVPQAYCRSPFEDKYKSGMSKMKHESDLSHLRYEMPASPFRSHSQQAFHSTRDLTMVQSPSSGMVGNKVKNEAVPQMSCQFAAAPSENLGLQKQDYGSAESQGPCLGASGREDTKVVVKNEYGGESCIRNTAGKEAEMFSKHLGQLQSPTLQSPLEKPHHHARKSIQSSPSPMIQPSYLPPQQDLSYSNHYFSGSYMHQGRQPSPGLCTLSQNPLVSGIPESPPEDHHLAMNCYRMTDRYHAYPNPLQHVGQEGHALETHGIDHLQNLASVSNHHLQNLEVNRPSGGHGVYPPFMGRPAPIQHYDHQPMNNPTFNHISQAPSPQGVMLPPQSYIAQPVSPHLTRNVGYGVPGLSRTGGFLPSPHEAFLSHPSPPVTPMTPPSTPRLTDLNPVRIDSPLHLRASYYSAATSPFGVPVDPSYSPLARSHSQGPAFAPYMDGAAWASDRDYNSAALQNSFYVPRSQCFPTAAPPLPQKDYAFRAPPGSHFAGPSLPSSSRLPPGLATPPQQDRSATASVLPPFKNTFLPAQSALNGFERHLQTFKSVMMEDAAQRSSASGLYGAPAPTGRPSQRPASHEGFTRTPLEPPVTEESSEGIYEVDSDNESAFQDEEVGGVAIALTHGSVLFECAKHELHATTALRRPNRKNPTRISLVFYQHKNLNFRNHGEEEWEKKMEVRKLERANGERPSKKKAKLSVDRLDEGGGFPAAPADVGQPWEVPPRVSLPSFFAINPYQKCF

Target ORF information:

RefSeq Version	XM_029982552.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982552.1

ORF Insert Sequence:

ATGCCCACCC CCGTAGACAA GATGGCGGCG CAGCACCCGG CGTCCCAGTT CGGCAGTCCG 
ATGCAGCATC ATCCCTTCGC CGACTTCCCC CCGCACCACG CGGTGTTCTT TGCGCCCGAC 
AAGGGCAACG CCGAAGAGCC GTCCTTCCAG GTGCCCTCCG TGTCGTCCAC CACGACTAGG 
CACACGCTGC TGCCCATGGT GCCGCTCCAG GAGCCCTGGG GAGACCAGCC GCCCATGGCC 
AGGAACGGCC TGGTGATGCA CCACCAGCAG CCGCAGCAGC AGCCGCCTCA CCACCACCAC 
CAGGGCGGGC CGCCCCAGCA GCAGCAGCAA CAGCAGCAGC AGCCCGGCAC CCCGGACGGC 
ATGGAGAAGG GCACCAAGAA GAAACGCAAA CGCTGCGGCG AGTGCCCTGG TTGCCTCAAG 
AAGGATAACT GCGGAGAGTG CGGGCCGTGC AGGAGTGTGC GCAGTCACCA GATCTGCAAG 
ATGCGGAAGT GCGATCAGCT CAAGACCAAA AAGGAGAAAG AGCGCTATGA GAAGATGATG 
CATCAGATGC ACGAAAGGCG TCGGGGTACA CCAGATGAAA ATAAAAACTT GCAACAGATC 
GGTGGCTTTC CCGGTCACAG CGAGTACCAG CTCCCGCCCA CGCAAAAGCC TGCCCCGCCG 
GCCCAGGCCG GCCAGATGTA CGAGATGGGG CCGCACTTCG GGGCCTTCCC GGGCCACCCG 
TCTCAGGCCC TGGCTTTCAA TGGCCACATG GCACAGCACG ACGCTGGCCG CCTGGGGCTC 
CCCGGCGAAG AGGGCGACTT CCCCACGCGC CACCAGATGA TGAACAGCCG TCTCAAGACA 
CTGATTCAGA CTAGGCAGAG CCAGAAGGAG CAGGGTCCCT TCCAGGGGCT CGAGACGACC 
CTAGGCCCCT CCGTCGGAGG AAGCTTTGTG CACCCGCAGG CCGATGCGAC GGCGACTGCC 
TCCCGGGGGT TCCCGCCGCT TGTGCGGAGC AACGGTGGCT GCGGTGCCGT GAGCGGCAAC 
AACAACGGCG GAAACAATGG CGGCGGCAAC AACAACAACA ACGGCACCAA CAACGGCGGT 
GGTAACAACG GCAACGGCGG CACGGGCGGC GGTAACAACA ATCCGAGGCC TCTGTCGTCA 
AACGAGATGC CCGGGGATGC GTGGCACGAT GCGAGCCCGT CCTCGGGTCC TCCGCCGGGC 
AGGGCTGGTG TCGCAGAGCT ACCGGAGTTT GCCAAGAACG GGTCCTCTTC CTCCTCCCAG 
GGAAGCAACA ACAACGCCAA CAACAACAAC TCCGAAGCTG AGTTCTCCCA GCCTGAGGAT 
CAGAGTCACG AGAGTGAGTT TGCAGAGGGT GGCGCTGCGG AAACCAGGGG AGGTGGCGGC 
GGCGACAGGT CGAGCGCTCC CCCCAACGGA ATGTTGCCCT ACGGAAGCTT CTATGGGGCG 
CAAGAGGCGG CAGAAGTCCG GAAGGGTGGC GAGGGCAAGG TCCCCCCGCC GACAACGAGC 
GCGGCGACGA CCACTTCGGC GGCGGACGTT CACGGTGGTG GTGCGCATTA CCAGTTCACG 
TCGGCAGCGA CTTCGATGAG CACGACGACG ACGAGTGCCT CCGTTCACGG CAAACCCTGC 
AACGGACCAA CGCAGCCTTC GTTTGCGGAG CGGCAGTCGC CCAGGGGCGG CCCGTTCTCC 
GGTGCACCGA CGTCATTCGC TCCGGCGCCC CACTTGGCGA GTATGCACCA AGAGCGCCCG 
GTCCCCGAAA GTCCGACGCA GCTCCGCAAC GAGGTAGGCG AAGCCGGCAG CGGCGGCCAG 
ACGCGCGGCA ATGAGAGCGA ATCCACGGAC GCGGCGGCAG TCACATCGCA GCCTTTTTTA 
TCGAATACGA CCACATCTAT GAATACATAT ACAAGTATTA TCAATACATA TAACAGCTTC 
GCCGCTTCGT GCGCGCCGTC CTTCTTGGCC GAGCACCAGC AACCCAAGTT TGGCGGGAGC 
GTGGAGGATG CGGCTCGAAC GGGGGGAGGA GACGCATCCG ACGTAGATGG TGGTCGTATT 
GCCGTCGGCG AATTCCGTGG ACTCGACTTT GGGGCACCCC CCCTGCCTCA CCACCCCGGT 
GGCCCCGAGG GCCCCAGGCC GCACTACCCC CAGTACGGGG GGGCGCCTGC CGCGTTTGTG 
CCAGAGGGTC CTTACGCTGT TCCCTATGGG GCCGGGAACC TGGGGAATCT GGGGAACCCG 
TTCCCCTTGC CCCCCATGGG CGCCGGAGGT CCTCCCTTTG CGCCCATGAG CCTCCCTGGT 
GCCGACCCCT GGTGGGAGCG GCTGGAGCGG CTACGCAGCA ACGCCAAGGC GGAGCCTCCG 
GCCTGCGACT GCTATGGCGC GGATGAGACG CCTCCCGTGG ATAAATCCCC ATACTATACC 
CACCTTGGTG CTGGACCGAC TGTGGCTGCT ATTAGAGAGA TGCTTGAGCA CAGGCTGAAC 
GAACGGGGCC CTGCGTTGCG CATCGAGAAG GTGCTCTACT CGGGCAAGGA GGGCAAGACG 
AGCCAGGGCT GCCCGGTGGC CAAGTGGATC ATCCGGCGCA GTGGCCCTTC GGAGAAGGTG 
CTGGCCGTGC TGCGGCACCG ACCGGGCCAC CGCTGCTTGT CGGCCTACAT CGTGATGGCC 
ATCGTCGCCT GGGAGGGCGT CCAGGCAGAC ATGGCAGACG ACCTCTACAG GACCGTGACC 
CACAAGACCG TCAACTTTGG CTTCCCCACT CAGCGGAGAT GTGGCACCAA CGAACAACGA 
ACCTGCGCCT GTCAAGGTGC CGACGCCGAG AACTGTGGCG CTTCCTTTTC ATTTGGCTGC 
TCTTGGAGCA TGTATTACAA TGGCTGCAAG TATGCACGGA GCAAGGCTGT CCGCAAGTTC 
AAGCTCTCAG AACAGTCCCA GGAGCAAGAG CTTGAAGACA AAATGCAGAT GCTCGCCACT 
GAAATGGCAC CGCTGTATGC AAGGGTGGCT CCGGAATCCT ACAAAAACCA GACCGAGTTT 
GAGAACGAAG GGATATCCTG CAGACTAGGG TTGAAGCAGG GAAGACCCTT TTCTGGCGTC 
ACGGCGTGTG TCGACTTCTG TGCCCATGCG CACAAGGACC TACACAACAT GAATAACGGC 
TGTACAGTTG TTGTAACCCT AACGAGACAT CGAGGATTTG ACAAGGGCGA CGACGAACAG 
CTGCACGTGC TTCCACTCTA CATACTGGAC GCAACCGACG AGCAGGGTGG CAAAGAAGGC 
TTTCATGAAA AGATCAAGAA TGGGTCACTC GAGGTTTTGT CGAAATTTCA CACGACGTCC 
AGGATAAGAA AGGTCCCCTT GGGGCCCTGC AAAAAGCGTG GAAGGAAAGA AAAGGTGACC 
GAGGAGCCGC TGCACTCAAT TCAGCAGTCT GCAAGTTCTG AGGCCCAAAC AGATGAAGGG 
TATGCTTCCC AAACCATGCC ATCACCAGTG TTCAGCCCCA GCTTCGAAGT GCCACAGGCG 
TATTGCAGAT CTCCTTTCGA AGACAAGTAC AAATCAGGGA TGAGCAAAAT GAAGCACGAG 
TCGGATCTCA GCCACCTGAG ATACGAGATG CCAGCAAGTC CCTTCCGATC TCACTCTCAG 
CAGGCCTTCC ACAGCACCAG GGACCTCACC ATGGTGCAGT CTCCTTCCAG CGGAATGGTC 
GGAAACAAGG TCAAGAATGA GGCTGTTCCT CAAATGTCTT GCCAGTTTGC TGCGGCTCCG 
AGCGAGAACC TTGGCTTGCA GAAGCAGGAC TATGGCAGTG CTGAATCTCA GGGTCCTTGT 
TTAGGAGCTT CCGGGAGGGA GGACACAAAG GTAGTAGTCA AAAACGAGTA TGGCGGCGAG 
TCCTGCATCC GGAACACTGC GGGTAAAGAG GCGGAAATGT TCAGCAAACA TCTCGGACAG 
TTACAATCAC CGACACTTCA GAGTCCTCTA GAAAAACCTC ATCATCATGC GCGAAAAAGC 
ATACAGAGTT CACCCAGCCC CATGATTCAG CCGTCCTATC TTCCACCTCA GCAAGACCTG 
AGTTACAGCA ACCATTACTT CAGCGGCAGC TACATGCATC AGGGCAGGCA GCCTAGCCCC 
GGCCTGTGCA CTCTTTCGCA AAATCCGCTC GTGTCTGGCA TTCCGGAGAG TCCCCCTGAA 
GACCATCACC TCGCAATGAA CTGCTACAGG ATGACCGACA GGTACCATGC CTACCCAAAC 
CCTCTTCAGC ACGTCGGTCA GGAAGGGCAC GCTCTGGAAA CTCACGGGAT AGACCACCTC 
CAGAACTTGG CGTCCGTCTC AAACCATCAC CTGCAGAACC TGGAGGTCAA CCGGCCTAGC 
GGGGGTCACG GCGTGTACCC GCCCTTCATG GGGAGGCCTG CGCCGATCCA GCACTACGAC 
CATCAACCCA TGAACAACCC CACGTTCAAC CACATCTCCC AGGCGCCTTC ACCGCAGGGG 
GTCATGCTTC CACCCCAGTC TTACATTGCC CAGCCCGTCT CGCCGCACCT GACGAGGAAC 
GTCGGCTACG GCGTGCCCGG GCTCTCCAGG ACCGGCGGCT TCCTCCCGTC TCCTCACGAG 
GCCTTCTTAT CTCACCCCAG TCCTCCCGTC ACCCCCATGA CTCCTCCCAG CACACCGAGG 
CTCACGGACC TCAATCCCGT CAGGATCGAC TCTCCGCTCC ACTTGCGAGC CTCTTACTAC 
TCGGCCGCGA CGAGTCCCTT CGGCGTGCCG GTCGACCCGT CCTACTCTCC GCTGGCCAGG 
AGCCACAGTC AGGGGCCCGC CTTTGCGCCA TACATGGACG GAGCGGCCTG GGCCTCCGAC 
CGTGACTACA ACAGTGCGGC TCTCCAAAAC TCCTTTTACG TGCCGCGCTC CCAGTGCTTC 
CCGACGGCAG CACCGCCGCT GCCCCAGAAA GACTATGCGT TCCGCGCGCC CCCGGGCAGC 
CACTTTGCAG GACCTTCTCT TCCCTCTTCG TCGCGTCTTC CGCCCGGCCT GGCCACGCCT 
CCGCAGCAGG ACCGCTCTGC CACCGCCAGC GTGCTTCCCC CGTTCAAGAA CACGTTTCTC 
CCGGCGCAGT CTGCCCTCAA CGGTTTCGAG CGTCACCTGC AGACGTTCAA GTCGGTGATG 
ATGGAAGACG CCGCGCAGAG GAGCTCCGCC TCTGGGCTCT ACGGCGCTCC GGCCCCCACG 
GGCCGCCCTT CTCAGCGGCC AGCTAGTCAC GAGGGCTTCA CGAGGACGCC TCTCGAACCG 
CCCGTCACGG AAGAGTCTTC CGAGGGGATC TACGAGGTGG ACTCGGACAA CGAAAGCGCC 
TTCCAGGACG AAGAGGTTGG CGGCGTCGCC ATTGCGCTCA CTCACGGGTC GGTGCTCTTC 
GAGTGCGCCA AGCACGAGCT GCATGCGACG ACTGCTCTGC GGAGGCCCAA CCGCAAGAAC 
CCCACCCGCA TCAGCCTCGT GTTCTACCAG CACAAGAACC TCAACTTCCG GAATCACGGG 
GAGGAGGAGT GGGAGAAGAA GATGGAGGTT CGCAAGTTGG AAAGAGCCAA CGGGGAGCGG 
CCCTCCAAGA AGAAGGCCAA GCTCTCCGTG GACAGGTTGG ATGAGGGTGG CGGCTTCCCC 
GCGGCTCCCG CAGACGTCGG CCAGCCGTGG GAGGTGCCCC CCCGCGTGTC CCTGCCGTCG 
TTCTTCGCCA TCAACCCTTA CCAGAAGTGT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8042599 ( XM_029982553.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838413.1 Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OIa25175
Clone ID Related Accession (Same CDS sequence)	XM_029982553.1
Accession Version	XM_029982553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8042599 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919951.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACCC CCGTAGACAA GATGGCGGCG CAGCACCCGG CGTCCCAGTT CGGCAGTCCG 
ATGCAGCATC ATCCCTTCGC CGACTTCCCC CCGCACCACG CGGTGTTCTT TGCGCCCGAC 
AAGGGCAACG CCGAAGAGCC GTCCTTCCAG GTGCCCTCCG TGTCGTCCAC CACGACTAGG 
CACACGCTGC TGCCCATGGT GCCGCTCCAG GAGCCCTGGG GAGACCAGCC GCCCATGGCC 
AGGAACGGCC TGGTGATGCA CCACCAGCAG CCGCAGCAGC AGCCGCCTCA CCACCACCAC 
CAGGGCGGGC CGCCCCAGCA GCAGCAGCAA CAGCAGCAGC AGCCCGGCAC CCCGGACGGC 
ATGGAGAAGG GCACCAAGAA GAAACGCAAA CGCTGCGGCG AGTGCCCTGG TTGCCTCAAG 
AAGGATAACT GCGGAGAGTG CGGGCCGTGC AGGAGTGTGC GCAGTCACCA GATCTGCAAG 
ATGCGGAAGT GCGATCAGCT CAAGACCAAA AAGGAGAAAA TCGGTGGCTT TCCCGGTCAC 
AGCGAGTACC AGCTCCCGCC CACGCAAAAG CCTGCCCCGC CGGCCCAGGC CGGCCAGATG 
TACGAGATGG GGCCGCACTT CGGGGCCTTC CCGGGCCACC CGTCTCAGGC CCTGGCTTTC 
AATGGCCACA TGGCACAGCA CGACGCTGGC CGCCTGGGGC TCCCCGGCGA AGAGGGCGAC 
TTCCCCACGC GCCACCAGAT GATGAACAGC CGTCTCAAGA CACTGATTCA GACTAGGCAG 
AGCCAGAAGG AGCAGGGTCC CTTCCAGGGG CTCGAGACGA CCCTAGGCCC CTCCGTCGGA 
GGAAGCTTTG TGCACCCGCA GGCCGATGCG ACGGCGACTG CCTCCCGGGG GTTCCCGCCG 
CTTGTGCGGA GCAACGGTGG CTGCGGTGCC GTGAGCGGCA ACAACAACGG CGGAAACAAT 
GGCGGCGGCA ACAACAACAA CAACGGCACC AACAACGGCG GTGGTAACAA CGGCAACGGC 
GGCACGGGCG GCGGTAACAA CAATCCGAGG CCTCTGTCGT CAAACGAGAT GCCCGGGGAT 
GCGTGGCACG ATGCGAGCCC GTCCTCGGGT CCTCCGCCGG GCAGGGCTGG TGTCGCAGAG 
CTACCGGAGT TTGCCAAGAA CGGGTCCTCT TCCTCCTCCC AGGGAAGCAA CAACAACGCC 
AACAACAACA ACTCCGAAGC TGAGTTCTCC CAGCCTGAGG ATCAGAGTCA CGAGAGTGAG 
TTTGCAGAGG GTGGCGCTGC GGAAACCAGG GGAGGTGGCG GCGGCGACAG GTCGAGCGCT 
CCCCCCAACG GAATGTTGCC CTACGGAAGC TTCTATGGGG CGCAAGAGGC GGCAGAAGTC 
CGGAAGGGTG GCGAGGGCAA GGTCCCCCCG CCGACAACGA GCGCGGCGAC GACCACTTCG 
GCGGCGGACG TTCACGGTGG TGGTGCGCAT TACCAGTTCA CGTCGGCAGC GACTTCGATG 
AGCACGACGA CGACGAGTGC CTCCGTTCAC GGCAAACCCT GCAACGGACC AACGCAGCCT 
TCGTTTGCGG AGCGGCAGTC GCCCAGGGGC GGCCCGTTCT CCGGTGCACC GACGTCATTC 
GCTCCGGCGC CCCACTTGGC GAGTATGCAC CAAGAGCGCC CGGTCCCCGA AAGTCCGACG 
CAGCTCCGCA ACGAGGTAGG CGAAGCCGGC AGCGGCGGCC AGACGCGCGG CAATGAGAGC 
GAATCCACGG ACGCGGCGGC AGTCACATCG CAGCCTTTTT TATCGAATAC GACCACATCT 
ATGAATACAT ATACAAGTAT TATCAATACA TATAACAGCT TCGCCGCTTC GTGCGCGCCG 
TCCTTCTTGG CCGAGCACCA GCAACCCAAG TTTGGCGGGA GCGTGGAGGA TGCGGCTCGA 
ACGGGGGGAG GAGACGCATC CGACGTAGAT GGTGGTCGTA TTGCCGTCGG CGAATTCCGT 
GGACTCGACT TTGGGGCACC CCCCCTGCCT CACCACCCCG GTGGCCCCGA GGGCCCCAGG 
CCGCACTACC CCCAGTACGG GGGGGCGCCT GCCGCGTTTG TGCCAGAGGG TCCTTACGCT 
GTTCCCTATG GGGCCGGGAA CCTGGGGAAT CTGGGGAACC CGTTCCCCTT GCCCCCCATG 
GGCGCCGGAG GTCCTCCCTT TGCGCCCATG AGCCTCCCTG GTGCCGACCC CTGGTGGGAG 
CGGCTGGAGC GGCTACGCAG CAACGCCAAG GCGGAGCCTC CGGCCTGCGA CTGCTATGGC 
GCGGATGAGA CGCCTCCCGT GGATAAATCC CCATACTATA CCCACCTTGG TGCTGGACCG 
ACTGTGGCTG CTATTAGAGA GATGCTTGAG CACAGGCTGA ACGAACGGGG CCCTGCGTTG 
CGCATCGAGA AGGTGCTCTA CTCGGGCAAG GAGGGCAAGA CGAGCCAGGG CTGCCCGGTG 
GCCAAGTGGA TCATCCGGCG CAGTGGCCCT TCGGAGAAGG TGCTGGCCGT GCTGCGGCAC 
CGACCGGGCC ACCGCTGCTT GTCGGCCTAC ATCGTGATGG CCATCGTCGC CTGGGAGGGC 
GTCCAGGCAG ACATGGCAGA CGACCTCTAC AGGACCGTGA CCCACAAGAC CGTCAACTTT 
GGCTTCCCCA CTCAGCGGAG ATGTGGCACC AACGAACAAC GAACCTGCGC CTGTCAAGGT 
GCCGACGCCG AGAACTGTGG CGCTTCCTTT TCATTTGGCT GCTCTTGGAG CATGTATTAC 
AATGGCTGCA AGTATGCACG GAGCAAGGCT GTCCGCAAGT TCAAGCTCTC AGAACAGTCC 
CAGGAGCAAG AGCTTGAAGA CAAAATGCAG ATGCTCGCCA CTGAAATGGC ACCGCTGTAT 
GCAAGGGTGG CTCCGGAATC CTACAAAAAC CAGACCGAGT TTGAGAACGA AGGGATATCC 
TGCAGACTAG GGTTGAAGCA GGGAAGACCC TTTTCTGGCG TCACGGCGTG TGTCGACTTC 
TGTGCCCATG CGCACAAGGA CCTACACAAC ATGAATAACG GCTGTACAGT TGTTGTAACC 
CTAACGAGAC ATCGAGGATT TGACAAGGGC GACGACGAAC AGCTGCACGT GCTTCCACTC 
TACATACTGG ACGCAACCGA CGAGCAGGGT GGCAAAGAAG GCTTTCATGA AAAGATCAAG 
AATGGGTCAC TCGAGGTTTT GTCGAAATTT CACACGACGT CCAGGATAAG AAAGGTCCCC 
TTGGGGCCCT GCAAAAAGCG TGGAAGGAAA GAAAAGGTGA CCGAGGAGCC GCTGCACTCA 
ATTCAGCAGT CTGCAAGTTC TGAGGCCCAA ACAGATGAAG GGTATGCTTC CCAAACCATG 
CCATCACCAG TGTTCAGCCC CAGCTTCGAA GTGCCACAGG CGTATTGCAG ATCTCCTTTC 
GAAGACAAGT ACAAATCAGG GATGAGCAAA ATGAAGCACG AGTCGGATCT CAGCCACCTG 
AGATACGAGA TGCCAGCAAG TCCCTTCCGA TCTCACTCTC AGCAGGCCTT CCACAGCACC 
AGGGACCTCA CCATGGTGCA GTCTCCTTCC AGCGGAATGG TCGGAAACAA GGTCAAGAAT 
GAGGCTGTTC CTCAAATGTC TTGCCAGTTT GCTGCGGCTC CGAGCGAGAA CCTTGGCTTG 
CAGAAGCAGG ACTATGGCAG TGCTGAATCT CAGGGTCCTT GTTTAGGAGC TTCCGGGAGG 
GAGGACACAA AGGTAGTAGT CAAAAACGAG TATGGCGGCG AGTCCTGCAT CCGGAACACT 
GCGGGTAAAG AGGCGGAAAT GTTCAGCAAA CATCTCGGAC AGTTACAATC ACCGACACTT 
CAGAGTCCTC TAGAAAAACC TCATCATCAT GCGCGAAAAA GCATACAGAG TTCACCCAGC 
CCCATGATTC AGCCGTCCTA TCTTCCACCT CAGCAAGACC TGAGTTACAG CAACCATTAC 
TTCAGCGGCA GCTACATGCA TCAGGGCAGG CAGCCTAGCC CCGGCCTGTG CACTCTTTCG 
CAAAATCCGC TCGTGTCTGG CATTCCGGAG AGTCCCCCTG AAGACCATCA CCTCGCAATG 
AACTGCTACA GGATGACCGA CAGGTACCAT GCCTACCCAA ACCCTCTTCA GCACGTCGGT 
CAGGAAGGGC ACGCTCTGGA AACTCACGGG ATAGACCACC TCCAGAACTT GGCGTCCGTC 
TCAAACCATC ACCTGCAGAA CCTGGAGGTC AACCGGCCTA GCGGGGGTCA CGGCGTGTAC 
CCGCCCTTCA TGGGGAGGCC TGCGCCGATC CAGCACTACG ACCATCAACC CATGAACAAC 
CCCACGTTCA ACCACATCTC CCAGGCGCCT TCACCGCAGG GGGTCATGCT TCCACCCCAG 
TCTTACATTG CCCAGCCCGT CTCGCCGCAC CTGACGAGGA ACGTCGGCTA CGGCGTGCCC 
GGGCTCTCCA GGACCGGCGG CTTCCTCCCG TCTCCTCACG AGGCCTTCTT ATCTCACCCC 
AGTCCTCCCG TCACCCCCAT GACTCCTCCC AGCACACCGA GGCTCACGGA CCTCAATCCC 
GTCAGGATCG ACTCTCCGCT CCACTTGCGA GCCTCTTACT ACTCGGCCGC GACGAGTCCC 
TTCGGCGTGC CGGTCGACCC GTCCTACTCT CCGCTGGCCA GGAGCCACAG TCAGGGGCCC 
GCCTTTGCGC CATACATGGA CGGAGCGGCC TGGGCCTCCG ACCGTGACTA CAACAGTGCG 
GCTCTCCAAA ACTCCTTTTA CGTGCCGCGC TCCCAGTGCT TCCCGACGGC AGCACCGCCG 
CTGCCCCAGA AAGACTATGC GTTCCGCGCG CCCCCGGGCA GCCACTTTGC AGGACCTTCT 
CTTCCCTCTT CGTCGCGTCT TCCGCCCGGC CTGGCCACGC CTCCGCAGCA GGACCGCTCT 
GCCACCGCCA GCGTGCTTCC CCCGTTCAAG AACACGTTTC TCCCGGCGCA GTCTGCCCTC 
AACGGTTTCG AGCGTCACCT GCAGACGTTC AAGTCGGTGA TGATGGAAGA CGCCGCGCAG 
AGGAGCTCCG CCTCTGGGCT CTACGGCGCT CCGGCCCCCA CGGGCCGCCC TTCTCAGCGG 
CCAGCTAGTC ACGAGGGCTT CACGAGGACG CCTCTCGAAC CGCCCGTCAC GGAAGAGTCT 
TCCGAGGGGA TCTACGAGGT GGACTCGGAC AACGAAAGCG CCTTCCAGGA CGAAGAGGTT 
GGCGGCGTCG CCATTGCGCT CACTCACGGG TCGGTGCTCT TCGAGTGCGC CAAGCACGAG 
CTGCATGCGA CGACTGCTCT GCGGAGGCCC AACCGCAAGA ACCCCACCCG CATCAGCCTC 
GTGTTCTACC AGCACAAGAA CCTCAACTTC CGGAATCACG GGGAGGAGGA GTGGGAGAAG 
AAGATGGAGG TTCGCAAGTT GGAAAGAGCC AACGGGGAGC GGCCCTCCAA GAAGAAGGCC 
AAGCTCTCCG TGGACAGGTT GGATGAGGGT GGCGGCTTCC CCGCGGCTCC CGCAGACGTC 
GGCCAGCCGT GGGAGGTGCC CCCCCGCGTG TCCCTGCCGT CGTTCTTCGC CATCAACCCT 
TACCAGAAGT GTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838413.1
CDS	102..5699
Translation	MPTPVDKMAAQHPASQFGSPMQHHPFADFPPHHAVFFAPDKGNAEEPSFQVPSVSSTTTRHTLLPMVPLQEPWGDQPPMARNGLVMHHQQPQQQPPHHHHQGGPPQQQQQQQQQPGTPDGMEKGTKKKRKRCGECPGCLKKDNCGECGPCRSVRSHQICKMRKCDQLKTKKEKIGGFPGHSEYQLPPTQKPAPPAQAGQMYEMGPHFGAFPGHPSQALAFNGHMAQHDAGRLGLPGEEGDFPTRHQMMNSRLKTLIQTRQSQKEQGPFQGLETTLGPSVGGSFVHPQADATATASRGFPPLVRSNGGCGAVSGNNNGGNNGGGNNNNNGTNNGGGNNGNGGTGGGNNNPRPLSSNEMPGDAWHDASPSSGPPPGRAGVAELPEFAKNGSSSSSQGSNNNANNNNSEAEFSQPEDQSHESEFAEGGAAETRGGGGGDRSSAPPNGMLPYGSFYGAQEAAEVRKGGEGKVPPPTTSAATTTSAADVHGGGAHYQFTSAATSMSTTTTSASVHGKPCNGPTQPSFAERQSPRGGPFSGAPTSFAPAPHLASMHQERPVPESPTQLRNEVGEAGSGGQTRGNESESTDAAAVTSQPFLSNTTTSMNTYTSIINTYNSFAASCAPSFLAEHQQPKFGGSVEDAARTGGGDASDVDGGRIAVGEFRGLDFGAPPLPHHPGGPEGPRPHYPQYGGAPAAFVPEGPYAVPYGAGNLGNLGNPFPLPPMGAGGPPFAPMSLPGADPWWERLERLRSNAKAEPPACDCYGADETPPVDKSPYYTHLGAGPTVAAIREMLEHRLNERGPALRIEKVLYSGKEGKTSQGCPVAKWIIRRSGPSEKVLAVLRHRPGHRCLSAYIVMAIVAWEGVQADMADDLYRTVTHKTVNFGFPTQRRCGTNEQRTCACQGADAENCGASFSFGCSWSMYYNGCKYARSKAVRKFKLSEQSQEQELEDKMQMLATEMAPLYARVAPESYKNQTEFENEGISCRLGLKQGRPFSGVTACVDFCAHAHKDLHNMNNGCTVVVTLTRHRGFDKGDDEQLHVLPLYILDATDEQGGKEGFHEKIKNGSLEVLSKFHTTSRIRKVPLGPCKKRGRKEKVTEEPLHSIQQSASSEAQTDEGYASQTMPSPVFSPSFEVPQAYCRSPFEDKYKSGMSKMKHESDLSHLRYEMPASPFRSHSQQAFHSTRDLTMVQSPSSGMVGNKVKNEAVPQMSCQFAAAPSENLGLQKQDYGSAESQGPCLGASGREDTKVVVKNEYGGESCIRNTAGKEAEMFSKHLGQLQSPTLQSPLEKPHHHARKSIQSSPSPMIQPSYLPPQQDLSYSNHYFSGSYMHQGRQPSPGLCTLSQNPLVSGIPESPPEDHHLAMNCYRMTDRYHAYPNPLQHVGQEGHALETHGIDHLQNLASVSNHHLQNLEVNRPSGGHGVYPPFMGRPAPIQHYDHQPMNNPTFNHISQAPSPQGVMLPPQSYIAQPVSPHLTRNVGYGVPGLSRTGGFLPSPHEAFLSHPSPPVTPMTPPSTPRLTDLNPVRIDSPLHLRASYYSAATSPFGVPVDPSYSPLARSHSQGPAFAPYMDGAAWASDRDYNSAALQNSFYVPRSQCFPTAAPPLPQKDYAFRAPPGSHFAGPSLPSSSRLPPGLATPPQQDRSATASVLPPFKNTFLPAQSALNGFERHLQTFKSVMMEDAAQRSSASGLYGAPAPTGRPSQRPASHEGFTRTPLEPPVTEESSEGIYEVDSDNESAFQDEEVGGVAIALTHGSVLFECAKHELHATTALRRPNRKNPTRISLVFYQHKNLNFRNHGEEEWEKKMEVRKLERANGERPSKKKAKLSVDRLDEGGGFPAAPADVGQPWEVPPRVSLPSFFAINPYQKCF

Target ORF information:

RefSeq Version	XM_029982553.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982553.1

ORF Insert Sequence:

ATGCCCACCC CCGTAGACAA GATGGCGGCG CAGCACCCGG CGTCCCAGTT CGGCAGTCCG 
ATGCAGCATC ATCCCTTCGC CGACTTCCCC CCGCACCACG CGGTGTTCTT TGCGCCCGAC 
AAGGGCAACG CCGAAGAGCC GTCCTTCCAG GTGCCCTCCG TGTCGTCCAC CACGACTAGG 
CACACGCTGC TGCCCATGGT GCCGCTCCAG GAGCCCTGGG GAGACCAGCC GCCCATGGCC 
AGGAACGGCC TGGTGATGCA CCACCAGCAG CCGCAGCAGC AGCCGCCTCA CCACCACCAC 
CAGGGCGGGC CGCCCCAGCA GCAGCAGCAA CAGCAGCAGC AGCCCGGCAC CCCGGACGGC 
ATGGAGAAGG GCACCAAGAA GAAACGCAAA CGCTGCGGCG AGTGCCCTGG TTGCCTCAAG 
AAGGATAACT GCGGAGAGTG CGGGCCGTGC AGGAGTGTGC GCAGTCACCA GATCTGCAAG 
ATGCGGAAGT GCGATCAGCT CAAGACCAAA AAGGAGAAAA TCGGTGGCTT TCCCGGTCAC 
AGCGAGTACC AGCTCCCGCC CACGCAAAAG CCTGCCCCGC CGGCCCAGGC CGGCCAGATG 
TACGAGATGG GGCCGCACTT CGGGGCCTTC CCGGGCCACC CGTCTCAGGC CCTGGCTTTC 
AATGGCCACA TGGCACAGCA CGACGCTGGC CGCCTGGGGC TCCCCGGCGA AGAGGGCGAC 
TTCCCCACGC GCCACCAGAT GATGAACAGC CGTCTCAAGA CACTGATTCA GACTAGGCAG 
AGCCAGAAGG AGCAGGGTCC CTTCCAGGGG CTCGAGACGA CCCTAGGCCC CTCCGTCGGA 
GGAAGCTTTG TGCACCCGCA GGCCGATGCG ACGGCGACTG CCTCCCGGGG GTTCCCGCCG 
CTTGTGCGGA GCAACGGTGG CTGCGGTGCC GTGAGCGGCA ACAACAACGG CGGAAACAAT 
GGCGGCGGCA ACAACAACAA CAACGGCACC AACAACGGCG GTGGTAACAA CGGCAACGGC 
GGCACGGGCG GCGGTAACAA CAATCCGAGG CCTCTGTCGT CAAACGAGAT GCCCGGGGAT 
GCGTGGCACG ATGCGAGCCC GTCCTCGGGT CCTCCGCCGG GCAGGGCTGG TGTCGCAGAG 
CTACCGGAGT TTGCCAAGAA CGGGTCCTCT TCCTCCTCCC AGGGAAGCAA CAACAACGCC 
AACAACAACA ACTCCGAAGC TGAGTTCTCC CAGCCTGAGG ATCAGAGTCA CGAGAGTGAG 
TTTGCAGAGG GTGGCGCTGC GGAAACCAGG GGAGGTGGCG GCGGCGACAG GTCGAGCGCT 
CCCCCCAACG GAATGTTGCC CTACGGAAGC TTCTATGGGG CGCAAGAGGC GGCAGAAGTC 
CGGAAGGGTG GCGAGGGCAA GGTCCCCCCG CCGACAACGA GCGCGGCGAC GACCACTTCG 
GCGGCGGACG TTCACGGTGG TGGTGCGCAT TACCAGTTCA CGTCGGCAGC GACTTCGATG 
AGCACGACGA CGACGAGTGC CTCCGTTCAC GGCAAACCCT GCAACGGACC AACGCAGCCT 
TCGTTTGCGG AGCGGCAGTC GCCCAGGGGC GGCCCGTTCT CCGGTGCACC GACGTCATTC 
GCTCCGGCGC CCCACTTGGC GAGTATGCAC CAAGAGCGCC CGGTCCCCGA AAGTCCGACG 
CAGCTCCGCA ACGAGGTAGG CGAAGCCGGC AGCGGCGGCC AGACGCGCGG CAATGAGAGC 
GAATCCACGG ACGCGGCGGC AGTCACATCG CAGCCTTTTT TATCGAATAC GACCACATCT 
ATGAATACAT ATACAAGTAT TATCAATACA TATAACAGCT TCGCCGCTTC GTGCGCGCCG 
TCCTTCTTGG CCGAGCACCA GCAACCCAAG TTTGGCGGGA GCGTGGAGGA TGCGGCTCGA 
ACGGGGGGAG GAGACGCATC CGACGTAGAT GGTGGTCGTA TTGCCGTCGG CGAATTCCGT 
GGACTCGACT TTGGGGCACC CCCCCTGCCT CACCACCCCG GTGGCCCCGA GGGCCCCAGG 
CCGCACTACC CCCAGTACGG GGGGGCGCCT GCCGCGTTTG TGCCAGAGGG TCCTTACGCT 
GTTCCCTATG GGGCCGGGAA CCTGGGGAAT CTGGGGAACC CGTTCCCCTT GCCCCCCATG 
GGCGCCGGAG GTCCTCCCTT TGCGCCCATG AGCCTCCCTG GTGCCGACCC CTGGTGGGAG 
CGGCTGGAGC GGCTACGCAG CAACGCCAAG GCGGAGCCTC CGGCCTGCGA CTGCTATGGC 
GCGGATGAGA CGCCTCCCGT GGATAAATCC CCATACTATA CCCACCTTGG TGCTGGACCG 
ACTGTGGCTG CTATTAGAGA GATGCTTGAG CACAGGCTGA ACGAACGGGG CCCTGCGTTG 
CGCATCGAGA AGGTGCTCTA CTCGGGCAAG GAGGGCAAGA CGAGCCAGGG CTGCCCGGTG 
GCCAAGTGGA TCATCCGGCG CAGTGGCCCT TCGGAGAAGG TGCTGGCCGT GCTGCGGCAC 
CGACCGGGCC ACCGCTGCTT GTCGGCCTAC ATCGTGATGG CCATCGTCGC CTGGGAGGGC 
GTCCAGGCAG ACATGGCAGA CGACCTCTAC AGGACCGTGA CCCACAAGAC CGTCAACTTT 
GGCTTCCCCA CTCAGCGGAG ATGTGGCACC AACGAACAAC GAACCTGCGC CTGTCAAGGT 
GCCGACGCCG AGAACTGTGG CGCTTCCTTT TCATTTGGCT GCTCTTGGAG CATGTATTAC 
AATGGCTGCA AGTATGCACG GAGCAAGGCT GTCCGCAAGT TCAAGCTCTC AGAACAGTCC 
CAGGAGCAAG AGCTTGAAGA CAAAATGCAG ATGCTCGCCA CTGAAATGGC ACCGCTGTAT 
GCAAGGGTGG CTCCGGAATC CTACAAAAAC CAGACCGAGT TTGAGAACGA AGGGATATCC 
TGCAGACTAG GGTTGAAGCA GGGAAGACCC TTTTCTGGCG TCACGGCGTG TGTCGACTTC 
TGTGCCCATG CGCACAAGGA CCTACACAAC ATGAATAACG GCTGTACAGT TGTTGTAACC 
CTAACGAGAC ATCGAGGATT TGACAAGGGC GACGACGAAC AGCTGCACGT GCTTCCACTC 
TACATACTGG ACGCAACCGA CGAGCAGGGT GGCAAAGAAG GCTTTCATGA AAAGATCAAG 
AATGGGTCAC TCGAGGTTTT GTCGAAATTT CACACGACGT CCAGGATAAG AAAGGTCCCC 
TTGGGGCCCT GCAAAAAGCG TGGAAGGAAA GAAAAGGTGA CCGAGGAGCC GCTGCACTCA 
ATTCAGCAGT CTGCAAGTTC TGAGGCCCAA ACAGATGAAG GGTATGCTTC CCAAACCATG 
CCATCACCAG TGTTCAGCCC CAGCTTCGAA GTGCCACAGG CGTATTGCAG ATCTCCTTTC 
GAAGACAAGT ACAAATCAGG GATGAGCAAA ATGAAGCACG AGTCGGATCT CAGCCACCTG 
AGATACGAGA TGCCAGCAAG TCCCTTCCGA TCTCACTCTC AGCAGGCCTT CCACAGCACC 
AGGGACCTCA CCATGGTGCA GTCTCCTTCC AGCGGAATGG TCGGAAACAA GGTCAAGAAT 
GAGGCTGTTC CTCAAATGTC TTGCCAGTTT GCTGCGGCTC CGAGCGAGAA CCTTGGCTTG 
CAGAAGCAGG ACTATGGCAG TGCTGAATCT CAGGGTCCTT GTTTAGGAGC TTCCGGGAGG 
GAGGACACAA AGGTAGTAGT CAAAAACGAG TATGGCGGCG AGTCCTGCAT CCGGAACACT 
GCGGGTAAAG AGGCGGAAAT GTTCAGCAAA CATCTCGGAC AGTTACAATC ACCGACACTT 
CAGAGTCCTC TAGAAAAACC TCATCATCAT GCGCGAAAAA GCATACAGAG TTCACCCAGC 
CCCATGATTC AGCCGTCCTA TCTTCCACCT CAGCAAGACC TGAGTTACAG CAACCATTAC 
TTCAGCGGCA GCTACATGCA TCAGGGCAGG CAGCCTAGCC CCGGCCTGTG CACTCTTTCG 
CAAAATCCGC TCGTGTCTGG CATTCCGGAG AGTCCCCCTG AAGACCATCA CCTCGCAATG 
AACTGCTACA GGATGACCGA CAGGTACCAT GCCTACCCAA ACCCTCTTCA GCACGTCGGT 
CAGGAAGGGC ACGCTCTGGA AACTCACGGG ATAGACCACC TCCAGAACTT GGCGTCCGTC 
TCAAACCATC ACCTGCAGAA CCTGGAGGTC AACCGGCCTA GCGGGGGTCA CGGCGTGTAC 
CCGCCCTTCA TGGGGAGGCC TGCGCCGATC CAGCACTACG ACCATCAACC CATGAACAAC 
CCCACGTTCA ACCACATCTC CCAGGCGCCT TCACCGCAGG GGGTCATGCT TCCACCCCAG 
TCTTACATTG CCCAGCCCGT CTCGCCGCAC CTGACGAGGA ACGTCGGCTA CGGCGTGCCC 
GGGCTCTCCA GGACCGGCGG CTTCCTCCCG TCTCCTCACG AGGCCTTCTT ATCTCACCCC 
AGTCCTCCCG TCACCCCCAT GACTCCTCCC AGCACACCGA GGCTCACGGA CCTCAATCCC 
GTCAGGATCG ACTCTCCGCT CCACTTGCGA GCCTCTTACT ACTCGGCCGC GACGAGTCCC 
TTCGGCGTGC CGGTCGACCC GTCCTACTCT CCGCTGGCCA GGAGCCACAG TCAGGGGCCC 
GCCTTTGCGC CATACATGGA CGGAGCGGCC TGGGCCTCCG ACCGTGACTA CAACAGTGCG 
GCTCTCCAAA ACTCCTTTTA CGTGCCGCGC TCCCAGTGCT TCCCGACGGC AGCACCGCCG 
CTGCCCCAGA AAGACTATGC GTTCCGCGCG CCCCCGGGCA GCCACTTTGC AGGACCTTCT 
CTTCCCTCTT CGTCGCGTCT TCCGCCCGGC CTGGCCACGC CTCCGCAGCA GGACCGCTCT 
GCCACCGCCA GCGTGCTTCC CCCGTTCAAG AACACGTTTC TCCCGGCGCA GTCTGCCCTC 
AACGGTTTCG AGCGTCACCT GCAGACGTTC AAGTCGGTGA TGATGGAAGA CGCCGCGCAG 
AGGAGCTCCG CCTCTGGGCT CTACGGCGCT CCGGCCCCCA CGGGCCGCCC TTCTCAGCGG 
CCAGCTAGTC ACGAGGGCTT CACGAGGACG CCTCTCGAAC CGCCCGTCAC GGAAGAGTCT 
TCCGAGGGGA TCTACGAGGT GGACTCGGAC AACGAAAGCG CCTTCCAGGA CGAAGAGGTT 
GGCGGCGTCG CCATTGCGCT CACTCACGGG TCGGTGCTCT TCGAGTGCGC CAAGCACGAG 
CTGCATGCGA CGACTGCTCT GCGGAGGCCC AACCGCAAGA ACCCCACCCG CATCAGCCTC 
GTGTTCTACC AGCACAAGAA CCTCAACTTC CGGAATCACG GGGAGGAGGA GTGGGAGAAG 
AAGATGGAGG TTCGCAAGTT GGAAAGAGCC AACGGGGAGC GGCCCTCCAA GAAGAAGGCC 
AAGCTCTCCG TGGACAGGTT GGATGAGGGT GGCGGCTTCC CCGCGGCTCC CGCAGACGTC 
GGCCAGCCGT GGGAGGTGCC CCCCCGCGTG TCCCTGCCGT CGTTCTTCGC CATCAACCCT 
TACCAGAAGT GTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8042599 ( XM_029982555.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838415.1 Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OIa25176
Clone ID Related Accession (Same CDS sequence)	XM_029982555.1 , XM_029982556.1
Accession Version	XM_029982555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8042599 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919951.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCATC AGATGCACGA AAGGCGTCGG GGTACACCAG ATGAAAATAA AAACTTGCAA 
CAGGTAGCGA GCGCCATTTG CGGAAGCGGG GTCGCCGGAA AAGACGGCAT GGCGGCCTCT 
CCGGGCAACC GAACTAATCT CAACGGGCGG GCGGTGAACG GCGGCGGCGC TTCGACGTCG 
CCCTACCTCC CGCAGGGAGT GCCGGAACAC CTCGGACATC CTGGGATCGG TGGCTTTCCC 
GGTCACAGCG AGTACCAGCT CCCGCCCACG CAAAAGCCTG CCCCGCCGGC CCAGGCCGGC 
CAGATGTACG AGATGGGGCC GCACTTCGGG GCCTTCCCGG GCCACCCGTC TCAGGCCCTG 
GCTTTCAATG GCCACATGGC ACAGCACGAC GCTGGCCGCC TGGGGCTCCC CGGCGAAGAG 
GGCGACTTCC CCACGCGCCA CCAGATGATG AACAGCCGTC TCAAGACACT GATTCAGACT 
AGGCAGAGCC AGAAGGAGCA GGGTCCCTTC CAGGGGCTCG AGACGACCCT AGGCCCCTCC 
GTCGGAGGAA GCTTTGTGCA CCCGCAGGCC GATGCGACGG CGACTGCCTC CCGGGGGTTC 
CCGCCGCTTG TGCGGAGCAA CGGTGGCTGC GGTGCCGTGA GCGGCAACAA CAACGGCGGA 
AACAATGGCG GCGGCAACAA CAACAACAAC GGCACCAACA ACGGCGGTGG TAACAACGGC 
AACGGCGGCA CGGGCGGCGG TAACAACAAT CCGAGGCCTC TGTCGTCAAA CGAGATGCCC 
GGGGATGCGT GGCACGATGC GAGCCCGTCC TCGGGTCCTC CGCCGGGCAG GGCTGGTGTC 
GCAGAGCTAC CGGAGTTTGC CAAGAACGGG TCCTCTTCCT CCTCCCAGGG AAGCAACAAC 
AACGCCAACA ACAACAACTC CGAAGCTGAG TTCTCCCAGC CTGAGGATCA GAGTCACGAG 
AGTGAGTTTG CAGAGGGTGG CGCTGCGGAA ACCAGGGGAG GTGGCGGCGG CGACAGGTCG 
AGCGCTCCCC CCAACGGAAT GTTGCCCTAC GGAAGCTTCT ATGGGGCGCA AGAGGCGGCA 
GAAGTCCGGA AGGGTGGCGA GGGCAAGGTC CCCCCGCCGA CAACGAGCGC GGCGACGACC 
ACTTCGGCGG CGGACGTTCA CGGTGGTGGT GCGCATTACC AGTTCACGTC GGCAGCGACT 
TCGATGAGCA CGACGACGAC GAGTGCCTCC GTTCACGGCA AACCCTGCAA CGGACCAACG 
CAGCCTTCGT TTGCGGAGCG GCAGTCGCCC AGGGGCGGCC CGTTCTCCGG TGCACCGACG 
TCATTCGCTC CGGCGCCCCA CTTGGCGAGT ATGCACCAAG AGCGCCCGGT CCCCGAAAGT 
CCGACGCAGC TCCGCAACGA GGTAGGCGAA GCCGGCAGCG GCGGCCAGAC GCGCGGCAAT 
GAGAGCGAAT CCACGGACGC GGCGGCAGTC ACATCGCAGC CTTTTTTATC GAATACGACC 
ACATCTATGA ATACATATAC AAGTATTATC AATACATATA ACAGCTTCGC CGCTTCGTGC 
GCGCCGTCCT TCTTGGCCGA GCACCAGCAA CCCAAGTTTG GCGGGAGCGT GGAGGATGCG 
GCTCGAACGG GGGGAGGAGA CGCATCCGAC GTAGATGGTG GTCGTATTGC CGTCGGCGAA 
TTCCGTGGAC TCGACTTTGG GGCACCCCCC CTGCCTCACC ACCCCGGTGG CCCCGAGGGC 
CCCAGGCCGC ACTACCCCCA GTACGGGGGG GCGCCTGCCG CGTTTGTGCC AGAGGGTCCT 
TACGCTGTTC CCTATGGGGC CGGGAACCTG GGGAATCTGG GGAACCCGTT CCCCTTGCCC 
CCCATGGGCG CCGGAGGTCC TCCCTTTGCG CCCATGAGCC TCCCTGGTGC CGACCCCTGG 
TGGGAGCGGC TGGAGCGGCT ACGCAGCAAC GCCAAGGCGG AGCCTCCGGC CTGCGACTGC 
TATGGCGCGG ATGAGACGCC TCCCGTGGAT AAATCCCCAT ACTATACCCA CCTTGGTGCT 
GGACCGACTG TGGCTGCTAT TAGAGAGATG CTTGAGCACA GGCTGAACGA ACGGGGCCCT 
GCGTTGCGCA TCGAGAAGGT GCTCTACTCG GGCAAGGAGG GCAAGACGAG CCAGGGCTGC 
CCGGTGGCCA AGTGGATCAT CCGGCGCAGT GGCCCTTCGG AGAAGGTGCT GGCCGTGCTG 
CGGCACCGAC CGGGCCACCG CTGCTTGTCG GCCTACATCG TGATGGCCAT CGTCGCCTGG 
GAGGGCGTCC AGGCAGACAT GGCAGACGAC CTCTACAGGA CCGTGACCCA CAAGACCGTC 
AACTTTGGCT TCCCCACTCA GCGGAGATGT GGCACCAACG AACAACGAAC CTGCGCCTGT 
CAAGGTGCCG ACGCCGAGAA CTGTGGCGCT TCCTTTTCAT TTGGCTGCTC TTGGAGCATG 
TATTACAATG GCTGCAAGTA TGCACGGAGC AAGGCTGTCC GCAAGTTCAA GCTCTCAGAA 
CAGTCCCAGG AGCAAGAGCT TGAAGACAAA ATGCAGATGC TCGCCACTGA AATGGCACCG 
CTGTATGCAA GGGTGGCTCC GGAATCCTAC AAAAACCAGA CCGAGTTTGA GAACGAAGGG 
ATATCCTGCA GACTAGGGTT GAAGCAGGGA AGACCCTTTT CTGGCGTCAC GGCGTGTGTC 
GACTTCTGTG CCCATGCGCA CAAGGACCTA CACAACATGA ATAACGGCTG TACAGTTGTT 
GTAACCCTAA CGAGACATCG AGGATTTGAC AAGGGCGACG ACGAACAGCT GCACGTGCTT 
CCACTCTACA TACTGGACGC AACCGACGAG CAGGGTGGCA AAGAAGGCTT TCATGAAAAG 
ATCAAGAATG GGTCACTCGA GGTTTTGTCG AAATTTCACA CGACGTCCAG GATAAGAAAG 
GTCCCCTTGG GGCCCTGCAA AAAGCGTGGA AGGAAAGAAA AGGTGACCGA GGAGCCGCTG 
CACTCAATTC AGCAGTCTGC AAGTTCTGAG GCCCAAACAG ATGAAGGGTA TGCTTCCCAA 
ACCATGCCAT CACCAGTGTT CAGCCCCAGC TTCGAAGTGC CACAGGCGTA TTGCAGATCT 
CCTTTCGAAG ACAAGTACAA ATCAGGGATG AGCAAAATGA AGCACGAGTC GGATCTCAGC 
CACCTGAGAT ACGAGATGCC AGCAAGTCCC TTCCGATCTC ACTCTCAGCA GGCCTTCCAC 
AGCACCAGGG ACCTCACCAT GGTGCAGTCT CCTTCCAGCG GAATGGTCGG AAACAAGGTC 
AAGAATGAGG CTGTTCCTCA AATGTCTTGC CAGTTTGCTG CGGCTCCGAG CGAGAACCTT 
GGCTTGCAGA AGCAGGACTA TGGCAGTGCT GAATCTCAGG GTCCTTGTTT AGGAGCTTCC 
GGGAGGGAGG ACACAAAGGT AGTAGTCAAA AACGAGTATG GCGGCGAGTC CTGCATCCGG 
AACACTGCGG GTAAAGAGGC GGAAATGTTC AGCAAACATC TCGGACAGTT ACAATCACCG 
ACACTTCAGA GTCCTCTAGA AAAACCTCAT CATCATGCGC GAAAAAGCAT ACAGAGTTCA 
CCCAGCCCCA TGATTCAGCC GTCCTATCTT CCACCTCAGC AAGACCTGAG TTACAGCAAC 
CATTACTTCA GCGGCAGCTA CATGCATCAG GGCAGGCAGC CTAGCCCCGG CCTGTGCACT 
CTTTCGCAAA ATCCGCTCGT GTCTGGCATT CCGGAGAGTC CCCCTGAAGA CCATCACCTC 
GCAATGAACT GCTACAGGAT GACCGACAGG TACCATGCCT ACCCAAACCC TCTTCAGCAC 
GTCGGTCAGG AAGGGCACGC TCTGGAAACT CACGGGATAG ACCACCTCCA GAACTTGGCG 
TCCGTCTCAA ACCATCACCT GCAGAACCTG GAGGTCAACC GGCCTAGCGG GGGTCACGGC 
GTGTACCCGC CCTTCATGGG GAGGCCTGCG CCGATCCAGC ACTACGACCA TCAACCCATG 
AACAACCCCA CGTTCAACCA CATCTCCCAG GCGCCTTCAC CGCAGGGGGT CATGCTTCCA 
CCCCAGTCTT ACATTGCCCA GCCCGTCTCG CCGCACCTGA CGAGGAACGT CGGCTACGGC 
GTGCCCGGGC TCTCCAGGAC CGGCGGCTTC CTCCCGTCTC CTCACGAGGC CTTCTTATCT 
CACCCCAGTC CTCCCGTCAC CCCCATGACT CCTCCCAGCA CACCGAGGCT CACGGACCTC 
AATCCCGTCA GGATCGACTC TCCGCTCCAC TTGCGAGCCT CTTACTACTC GGCCGCGACG 
AGTCCCTTCG GCGTGCCGGT CGACCCGTCC TACTCTCCGC TGGCCAGGAG CCACAGTCAG 
GGGCCCGCCT TTGCGCCATA CATGGACGGA GCGGCCTGGG CCTCCGACCG TGACTACAAC 
AGTGCGGCTC TCCAAAACTC CTTTTACGTG CCGCGCTCCC AGTGCTTCCC GACGGCAGCA 
CCGCCGCTGC CCCAGAAAGA CTATGCGTTC CGCGCGCCCC CGGGCAGCCA CTTTGCAGGA 
CCTTCTCTTC CCTCTTCGTC GCGTCTTCCG CCCGGCCTGG CCACGCCTCC GCAGCAGGAC 
CGCTCTGCCA CCGCCAGCGT GCTTCCCCCG TTCAAGAACA CGTTTCTCCC GGCGCAGTCT 
GCCCTCAACG GTTTCGAGCG TCACCTGCAG ACGTTCAAGT CGGTGATGAT GGAAGACGCC 
GCGCAGAGGA GCTCCGCCTC TGGGCTCTAC GGCGCTCCGG CCCCCACGGG CCGCCCTTCT 
CAGCGGCCAG CTAGTCACGA GGGCTTCACG AGGACGCCTC TCGAACCGCC CGTCACGGAA 
GAGTCTTCCG AGGGGATCTA CGAGGTGGAC TCGGACAACG AAAGCGCCTT CCAGGACGAA 
GAGGTTGGCG GCGTCGCCAT TGCGCTCACT CACGGGTCGG TGCTCTTCGA GTGCGCCAAG 
CACGAGCTGC ATGCGACGAC TGCTCTGCGG AGGCCCAACC GCAAGAACCC CACCCGCATC 
AGCCTCGTGT TCTACCAGCA CAAGAACCTC AACTTCCGGA ATCACGGGGA GGAGGAGTGG 
GAGAAGAAGA TGGAGGTTCG CAAGTTGGAA AGAGCCAACG GGGAGCGGCC CTCCAAGAAG 
AAGGCCAAGC TCTCCGTGGA CAGGTTGGAT GAGGGTGGCG GCTTCCCCGC GGCTCCCGCA 
GACGTCGGCC AGCCGTGGGA GGTGCCCCCC CGCGTGTCCC TGCCGTCGTT CTTCGCCATC 
AACCCTTACC AGAAGTGTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838415.1
CDS	105..5408
Translation	MMHQMHERRRGTPDENKNLQQVASAICGSGVAGKDGMAASPGNRTNLNGRAVNGGGASTSPYLPQGVPEHLGHPGIGGFPGHSEYQLPPTQKPAPPAQAGQMYEMGPHFGAFPGHPSQALAFNGHMAQHDAGRLGLPGEEGDFPTRHQMMNSRLKTLIQTRQSQKEQGPFQGLETTLGPSVGGSFVHPQADATATASRGFPPLVRSNGGCGAVSGNNNGGNNGGGNNNNNGTNNGGGNNGNGGTGGGNNNPRPLSSNEMPGDAWHDASPSSGPPPGRAGVAELPEFAKNGSSSSSQGSNNNANNNNSEAEFSQPEDQSHESEFAEGGAAETRGGGGGDRSSAPPNGMLPYGSFYGAQEAAEVRKGGEGKVPPPTTSAATTTSAADVHGGGAHYQFTSAATSMSTTTTSASVHGKPCNGPTQPSFAERQSPRGGPFSGAPTSFAPAPHLASMHQERPVPESPTQLRNEVGEAGSGGQTRGNESESTDAAAVTSQPFLSNTTTSMNTYTSIINTYNSFAASCAPSFLAEHQQPKFGGSVEDAARTGGGDASDVDGGRIAVGEFRGLDFGAPPLPHHPGGPEGPRPHYPQYGGAPAAFVPEGPYAVPYGAGNLGNLGNPFPLPPMGAGGPPFAPMSLPGADPWWERLERLRSNAKAEPPACDCYGADETPPVDKSPYYTHLGAGPTVAAIREMLEHRLNERGPALRIEKVLYSGKEGKTSQGCPVAKWIIRRSGPSEKVLAVLRHRPGHRCLSAYIVMAIVAWEGVQADMADDLYRTVTHKTVNFGFPTQRRCGTNEQRTCACQGADAENCGASFSFGCSWSMYYNGCKYARSKAVRKFKLSEQSQEQELEDKMQMLATEMAPLYARVAPESYKNQTEFENEGISCRLGLKQGRPFSGVTACVDFCAHAHKDLHNMNNGCTVVVTLTRHRGFDKGDDEQLHVLPLYILDATDEQGGKEGFHEKIKNGSLEVLSKFHTTSRIRKVPLGPCKKRGRKEKVTEEPLHSIQQSASSEAQTDEGYASQTMPSPVFSPSFEVPQAYCRSPFEDKYKSGMSKMKHESDLSHLRYEMPASPFRSHSQQAFHSTRDLTMVQSPSSGMVGNKVKNEAVPQMSCQFAAAPSENLGLQKQDYGSAESQGPCLGASGREDTKVVVKNEYGGESCIRNTAGKEAEMFSKHLGQLQSPTLQSPLEKPHHHARKSIQSSPSPMIQPSYLPPQQDLSYSNHYFSGSYMHQGRQPSPGLCTLSQNPLVSGIPESPPEDHHLAMNCYRMTDRYHAYPNPLQHVGQEGHALETHGIDHLQNLASVSNHHLQNLEVNRPSGGHGVYPPFMGRPAPIQHYDHQPMNNPTFNHISQAPSPQGVMLPPQSYIAQPVSPHLTRNVGYGVPGLSRTGGFLPSPHEAFLSHPSPPVTPMTPPSTPRLTDLNPVRIDSPLHLRASYYSAATSPFGVPVDPSYSPLARSHSQGPAFAPYMDGAAWASDRDYNSAALQNSFYVPRSQCFPTAAPPLPQKDYAFRAPPGSHFAGPSLPSSSRLPPGLATPPQQDRSATASVLPPFKNTFLPAQSALNGFERHLQTFKSVMMEDAAQRSSASGLYGAPAPTGRPSQRPASHEGFTRTPLEPPVTEESSEGIYEVDSDNESAFQDEEVGGVAIALTHGSVLFECAKHELHATTALRRPNRKNPTRISLVFYQHKNLNFRNHGEEEWEKKMEVRKLERANGERPSKKKAKLSVDRLDEGGGFPAAPADVGQPWEVPPRVSLPSFFAINPYQKCF

Target ORF information:

RefSeq Version	XM_029982555.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982555.1

ORF Insert Sequence:

ATGATGCATC AGATGCACGA AAGGCGTCGG GGTACACCAG ATGAAAATAA AAACTTGCAA 
CAGGTAGCGA GCGCCATTTG CGGAAGCGGG GTCGCCGGAA AAGACGGCAT GGCGGCCTCT 
CCGGGCAACC GAACTAATCT CAACGGGCGG GCGGTGAACG GCGGCGGCGC TTCGACGTCG 
CCCTACCTCC CGCAGGGAGT GCCGGAACAC CTCGGACATC CTGGGATCGG TGGCTTTCCC 
GGTCACAGCG AGTACCAGCT CCCGCCCACG CAAAAGCCTG CCCCGCCGGC CCAGGCCGGC 
CAGATGTACG AGATGGGGCC GCACTTCGGG GCCTTCCCGG GCCACCCGTC TCAGGCCCTG 
GCTTTCAATG GCCACATGGC ACAGCACGAC GCTGGCCGCC TGGGGCTCCC CGGCGAAGAG 
GGCGACTTCC CCACGCGCCA CCAGATGATG AACAGCCGTC TCAAGACACT GATTCAGACT 
AGGCAGAGCC AGAAGGAGCA GGGTCCCTTC CAGGGGCTCG AGACGACCCT AGGCCCCTCC 
GTCGGAGGAA GCTTTGTGCA CCCGCAGGCC GATGCGACGG CGACTGCCTC CCGGGGGTTC 
CCGCCGCTTG TGCGGAGCAA CGGTGGCTGC GGTGCCGTGA GCGGCAACAA CAACGGCGGA 
AACAATGGCG GCGGCAACAA CAACAACAAC GGCACCAACA ACGGCGGTGG TAACAACGGC 
AACGGCGGCA CGGGCGGCGG TAACAACAAT CCGAGGCCTC TGTCGTCAAA CGAGATGCCC 
GGGGATGCGT GGCACGATGC GAGCCCGTCC TCGGGTCCTC CGCCGGGCAG GGCTGGTGTC 
GCAGAGCTAC CGGAGTTTGC CAAGAACGGG TCCTCTTCCT CCTCCCAGGG AAGCAACAAC 
AACGCCAACA ACAACAACTC CGAAGCTGAG TTCTCCCAGC CTGAGGATCA GAGTCACGAG 
AGTGAGTTTG CAGAGGGTGG CGCTGCGGAA ACCAGGGGAG GTGGCGGCGG CGACAGGTCG 
AGCGCTCCCC CCAACGGAAT GTTGCCCTAC GGAAGCTTCT ATGGGGCGCA AGAGGCGGCA 
GAAGTCCGGA AGGGTGGCGA GGGCAAGGTC CCCCCGCCGA CAACGAGCGC GGCGACGACC 
ACTTCGGCGG CGGACGTTCA CGGTGGTGGT GCGCATTACC AGTTCACGTC GGCAGCGACT 
TCGATGAGCA CGACGACGAC GAGTGCCTCC GTTCACGGCA AACCCTGCAA CGGACCAACG 
CAGCCTTCGT TTGCGGAGCG GCAGTCGCCC AGGGGCGGCC CGTTCTCCGG TGCACCGACG 
TCATTCGCTC CGGCGCCCCA CTTGGCGAGT ATGCACCAAG AGCGCCCGGT CCCCGAAAGT 
CCGACGCAGC TCCGCAACGA GGTAGGCGAA GCCGGCAGCG GCGGCCAGAC GCGCGGCAAT 
GAGAGCGAAT CCACGGACGC GGCGGCAGTC ACATCGCAGC CTTTTTTATC GAATACGACC 
ACATCTATGA ATACATATAC AAGTATTATC AATACATATA ACAGCTTCGC CGCTTCGTGC 
GCGCCGTCCT TCTTGGCCGA GCACCAGCAA CCCAAGTTTG GCGGGAGCGT GGAGGATGCG 
GCTCGAACGG GGGGAGGAGA CGCATCCGAC GTAGATGGTG GTCGTATTGC CGTCGGCGAA 
TTCCGTGGAC TCGACTTTGG GGCACCCCCC CTGCCTCACC ACCCCGGTGG CCCCGAGGGC 
CCCAGGCCGC ACTACCCCCA GTACGGGGGG GCGCCTGCCG CGTTTGTGCC AGAGGGTCCT 
TACGCTGTTC CCTATGGGGC CGGGAACCTG GGGAATCTGG GGAACCCGTT CCCCTTGCCC 
CCCATGGGCG CCGGAGGTCC TCCCTTTGCG CCCATGAGCC TCCCTGGTGC CGACCCCTGG 
TGGGAGCGGC TGGAGCGGCT ACGCAGCAAC GCCAAGGCGG AGCCTCCGGC CTGCGACTGC 
TATGGCGCGG ATGAGACGCC TCCCGTGGAT AAATCCCCAT ACTATACCCA CCTTGGTGCT 
GGACCGACTG TGGCTGCTAT TAGAGAGATG CTTGAGCACA GGCTGAACGA ACGGGGCCCT 
GCGTTGCGCA TCGAGAAGGT GCTCTACTCG GGCAAGGAGG GCAAGACGAG CCAGGGCTGC 
CCGGTGGCCA AGTGGATCAT CCGGCGCAGT GGCCCTTCGG AGAAGGTGCT GGCCGTGCTG 
CGGCACCGAC CGGGCCACCG CTGCTTGTCG GCCTACATCG TGATGGCCAT CGTCGCCTGG 
GAGGGCGTCC AGGCAGACAT GGCAGACGAC CTCTACAGGA CCGTGACCCA CAAGACCGTC 
AACTTTGGCT TCCCCACTCA GCGGAGATGT GGCACCAACG AACAACGAAC CTGCGCCTGT 
CAAGGTGCCG ACGCCGAGAA CTGTGGCGCT TCCTTTTCAT TTGGCTGCTC TTGGAGCATG 
TATTACAATG GCTGCAAGTA TGCACGGAGC AAGGCTGTCC GCAAGTTCAA GCTCTCAGAA 
CAGTCCCAGG AGCAAGAGCT TGAAGACAAA ATGCAGATGC TCGCCACTGA AATGGCACCG 
CTGTATGCAA GGGTGGCTCC GGAATCCTAC AAAAACCAGA CCGAGTTTGA GAACGAAGGG 
ATATCCTGCA GACTAGGGTT GAAGCAGGGA AGACCCTTTT CTGGCGTCAC GGCGTGTGTC 
GACTTCTGTG CCCATGCGCA CAAGGACCTA CACAACATGA ATAACGGCTG TACAGTTGTT 
GTAACCCTAA CGAGACATCG AGGATTTGAC AAGGGCGACG ACGAACAGCT GCACGTGCTT 
CCACTCTACA TACTGGACGC AACCGACGAG CAGGGTGGCA AAGAAGGCTT TCATGAAAAG 
ATCAAGAATG GGTCACTCGA GGTTTTGTCG AAATTTCACA CGACGTCCAG GATAAGAAAG 
GTCCCCTTGG GGCCCTGCAA AAAGCGTGGA AGGAAAGAAA AGGTGACCGA GGAGCCGCTG 
CACTCAATTC AGCAGTCTGC AAGTTCTGAG GCCCAAACAG ATGAAGGGTA TGCTTCCCAA 
ACCATGCCAT CACCAGTGTT CAGCCCCAGC TTCGAAGTGC CACAGGCGTA TTGCAGATCT 
CCTTTCGAAG ACAAGTACAA ATCAGGGATG AGCAAAATGA AGCACGAGTC GGATCTCAGC 
CACCTGAGAT ACGAGATGCC AGCAAGTCCC TTCCGATCTC ACTCTCAGCA GGCCTTCCAC 
AGCACCAGGG ACCTCACCAT GGTGCAGTCT CCTTCCAGCG GAATGGTCGG AAACAAGGTC 
AAGAATGAGG CTGTTCCTCA AATGTCTTGC CAGTTTGCTG CGGCTCCGAG CGAGAACCTT 
GGCTTGCAGA AGCAGGACTA TGGCAGTGCT GAATCTCAGG GTCCTTGTTT AGGAGCTTCC 
GGGAGGGAGG ACACAAAGGT AGTAGTCAAA AACGAGTATG GCGGCGAGTC CTGCATCCGG 
AACACTGCGG GTAAAGAGGC GGAAATGTTC AGCAAACATC TCGGACAGTT ACAATCACCG 
ACACTTCAGA GTCCTCTAGA AAAACCTCAT CATCATGCGC GAAAAAGCAT ACAGAGTTCA 
CCCAGCCCCA TGATTCAGCC GTCCTATCTT CCACCTCAGC AAGACCTGAG TTACAGCAAC 
CATTACTTCA GCGGCAGCTA CATGCATCAG GGCAGGCAGC CTAGCCCCGG CCTGTGCACT 
CTTTCGCAAA ATCCGCTCGT GTCTGGCATT CCGGAGAGTC CCCCTGAAGA CCATCACCTC 
GCAATGAACT GCTACAGGAT GACCGACAGG TACCATGCCT ACCCAAACCC TCTTCAGCAC 
GTCGGTCAGG AAGGGCACGC TCTGGAAACT CACGGGATAG ACCACCTCCA GAACTTGGCG 
TCCGTCTCAA ACCATCACCT GCAGAACCTG GAGGTCAACC GGCCTAGCGG GGGTCACGGC 
GTGTACCCGC CCTTCATGGG GAGGCCTGCG CCGATCCAGC ACTACGACCA TCAACCCATG 
AACAACCCCA CGTTCAACCA CATCTCCCAG GCGCCTTCAC CGCAGGGGGT CATGCTTCCA 
CCCCAGTCTT ACATTGCCCA GCCCGTCTCG CCGCACCTGA CGAGGAACGT CGGCTACGGC 
GTGCCCGGGC TCTCCAGGAC CGGCGGCTTC CTCCCGTCTC CTCACGAGGC CTTCTTATCT 
CACCCCAGTC CTCCCGTCAC CCCCATGACT CCTCCCAGCA CACCGAGGCT CACGGACCTC 
AATCCCGTCA GGATCGACTC TCCGCTCCAC TTGCGAGCCT CTTACTACTC GGCCGCGACG 
AGTCCCTTCG GCGTGCCGGT CGACCCGTCC TACTCTCCGC TGGCCAGGAG CCACAGTCAG 
GGGCCCGCCT TTGCGCCATA CATGGACGGA GCGGCCTGGG CCTCCGACCG TGACTACAAC 
AGTGCGGCTC TCCAAAACTC CTTTTACGTG CCGCGCTCCC AGTGCTTCCC GACGGCAGCA 
CCGCCGCTGC CCCAGAAAGA CTATGCGTTC CGCGCGCCCC CGGGCAGCCA CTTTGCAGGA 
CCTTCTCTTC CCTCTTCGTC GCGTCTTCCG CCCGGCCTGG CCACGCCTCC GCAGCAGGAC 
CGCTCTGCCA CCGCCAGCGT GCTTCCCCCG TTCAAGAACA CGTTTCTCCC GGCGCAGTCT 
GCCCTCAACG GTTTCGAGCG TCACCTGCAG ACGTTCAAGT CGGTGATGAT GGAAGACGCC 
GCGCAGAGGA GCTCCGCCTC TGGGCTCTAC GGCGCTCCGG CCCCCACGGG CCGCCCTTCT 
CAGCGGCCAG CTAGTCACGA GGGCTTCACG AGGACGCCTC TCGAACCGCC CGTCACGGAA 
GAGTCTTCCG AGGGGATCTA CGAGGTGGAC TCGGACAACG AAAGCGCCTT CCAGGACGAA 
GAGGTTGGCG GCGTCGCCAT TGCGCTCACT CACGGGTCGG TGCTCTTCGA GTGCGCCAAG 
CACGAGCTGC ATGCGACGAC TGCTCTGCGG AGGCCCAACC GCAAGAACCC CACCCGCATC 
AGCCTCGTGT TCTACCAGCA CAAGAACCTC AACTTCCGGA ATCACGGGGA GGAGGAGTGG 
GAGAAGAAGA TGGAGGTTCG CAAGTTGGAA AGAGCCAACG GGGAGCGGCC CTCCAAGAAG 
AAGGCCAAGC TCTCCGTGGA CAGGTTGGAT GAGGGTGGCG GCTTCCCCGC GGCTCCCGCA 
GACGTCGGCC AGCCGTGGGA GGTGCCCCCC CGCGTGTCCC TGCCGTCGTT CTTCGCCATC 
AACCCTTACC AGAAGTGTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8042599 ( XM_029982556.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838416.1 Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X6, mRNA.

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CloneID	OIa25176
Clone ID Related Accession (Same CDS sequence)	XM_029982555.1 , XM_029982556.1
Accession Version	XM_029982556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8042599 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919951.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCATC AGATGCACGA AAGGCGTCGG GGTACACCAG ATGAAAATAA AAACTTGCAA 
CAGGTAGCGA GCGCCATTTG CGGAAGCGGG GTCGCCGGAA AAGACGGCAT GGCGGCCTCT 
CCGGGCAACC GAACTAATCT CAACGGGCGG GCGGTGAACG GCGGCGGCGC TTCGACGTCG 
CCCTACCTCC CGCAGGGAGT GCCGGAACAC CTCGGACATC CTGGGATCGG TGGCTTTCCC 
GGTCACAGCG AGTACCAGCT CCCGCCCACG CAAAAGCCTG CCCCGCCGGC CCAGGCCGGC 
CAGATGTACG AGATGGGGCC GCACTTCGGG GCCTTCCCGG GCCACCCGTC TCAGGCCCTG 
GCTTTCAATG GCCACATGGC ACAGCACGAC GCTGGCCGCC TGGGGCTCCC CGGCGAAGAG 
GGCGACTTCC CCACGCGCCA CCAGATGATG AACAGCCGTC TCAAGACACT GATTCAGACT 
AGGCAGAGCC AGAAGGAGCA GGGTCCCTTC CAGGGGCTCG AGACGACCCT AGGCCCCTCC 
GTCGGAGGAA GCTTTGTGCA CCCGCAGGCC GATGCGACGG CGACTGCCTC CCGGGGGTTC 
CCGCCGCTTG TGCGGAGCAA CGGTGGCTGC GGTGCCGTGA GCGGCAACAA CAACGGCGGA 
AACAATGGCG GCGGCAACAA CAACAACAAC GGCACCAACA ACGGCGGTGG TAACAACGGC 
AACGGCGGCA CGGGCGGCGG TAACAACAAT CCGAGGCCTC TGTCGTCAAA CGAGATGCCC 
GGGGATGCGT GGCACGATGC GAGCCCGTCC TCGGGTCCTC CGCCGGGCAG GGCTGGTGTC 
GCAGAGCTAC CGGAGTTTGC CAAGAACGGG TCCTCTTCCT CCTCCCAGGG AAGCAACAAC 
AACGCCAACA ACAACAACTC CGAAGCTGAG TTCTCCCAGC CTGAGGATCA GAGTCACGAG 
AGTGAGTTTG CAGAGGGTGG CGCTGCGGAA ACCAGGGGAG GTGGCGGCGG CGACAGGTCG 
AGCGCTCCCC CCAACGGAAT GTTGCCCTAC GGAAGCTTCT ATGGGGCGCA AGAGGCGGCA 
GAAGTCCGGA AGGGTGGCGA GGGCAAGGTC CCCCCGCCGA CAACGAGCGC GGCGACGACC 
ACTTCGGCGG CGGACGTTCA CGGTGGTGGT GCGCATTACC AGTTCACGTC GGCAGCGACT 
TCGATGAGCA CGACGACGAC GAGTGCCTCC GTTCACGGCA AACCCTGCAA CGGACCAACG 
CAGCCTTCGT TTGCGGAGCG GCAGTCGCCC AGGGGCGGCC CGTTCTCCGG TGCACCGACG 
TCATTCGCTC CGGCGCCCCA CTTGGCGAGT ATGCACCAAG AGCGCCCGGT CCCCGAAAGT 
CCGACGCAGC TCCGCAACGA GGTAGGCGAA GCCGGCAGCG GCGGCCAGAC GCGCGGCAAT 
GAGAGCGAAT CCACGGACGC GGCGGCAGTC ACATCGCAGC CTTTTTTATC GAATACGACC 
ACATCTATGA ATACATATAC AAGTATTATC AATACATATA ACAGCTTCGC CGCTTCGTGC 
GCGCCGTCCT TCTTGGCCGA GCACCAGCAA CCCAAGTTTG GCGGGAGCGT GGAGGATGCG 
GCTCGAACGG GGGGAGGAGA CGCATCCGAC GTAGATGGTG GTCGTATTGC CGTCGGCGAA 
TTCCGTGGAC TCGACTTTGG GGCACCCCCC CTGCCTCACC ACCCCGGTGG CCCCGAGGGC 
CCCAGGCCGC ACTACCCCCA GTACGGGGGG GCGCCTGCCG CGTTTGTGCC AGAGGGTCCT 
TACGCTGTTC CCTATGGGGC CGGGAACCTG GGGAATCTGG GGAACCCGTT CCCCTTGCCC 
CCCATGGGCG CCGGAGGTCC TCCCTTTGCG CCCATGAGCC TCCCTGGTGC CGACCCCTGG 
TGGGAGCGGC TGGAGCGGCT ACGCAGCAAC GCCAAGGCGG AGCCTCCGGC CTGCGACTGC 
TATGGCGCGG ATGAGACGCC TCCCGTGGAT AAATCCCCAT ACTATACCCA CCTTGGTGCT 
GGACCGACTG TGGCTGCTAT TAGAGAGATG CTTGAGCACA GGCTGAACGA ACGGGGCCCT 
GCGTTGCGCA TCGAGAAGGT GCTCTACTCG GGCAAGGAGG GCAAGACGAG CCAGGGCTGC 
CCGGTGGCCA AGTGGATCAT CCGGCGCAGT GGCCCTTCGG AGAAGGTGCT GGCCGTGCTG 
CGGCACCGAC CGGGCCACCG CTGCTTGTCG GCCTACATCG TGATGGCCAT CGTCGCCTGG 
GAGGGCGTCC AGGCAGACAT GGCAGACGAC CTCTACAGGA CCGTGACCCA CAAGACCGTC 
AACTTTGGCT TCCCCACTCA GCGGAGATGT GGCACCAACG AACAACGAAC CTGCGCCTGT 
CAAGGTGCCG ACGCCGAGAA CTGTGGCGCT TCCTTTTCAT TTGGCTGCTC TTGGAGCATG 
TATTACAATG GCTGCAAGTA TGCACGGAGC AAGGCTGTCC GCAAGTTCAA GCTCTCAGAA 
CAGTCCCAGG AGCAAGAGCT TGAAGACAAA ATGCAGATGC TCGCCACTGA AATGGCACCG 
CTGTATGCAA GGGTGGCTCC GGAATCCTAC AAAAACCAGA CCGAGTTTGA GAACGAAGGG 
ATATCCTGCA GACTAGGGTT GAAGCAGGGA AGACCCTTTT CTGGCGTCAC GGCGTGTGTC 
GACTTCTGTG CCCATGCGCA CAAGGACCTA CACAACATGA ATAACGGCTG TACAGTTGTT 
GTAACCCTAA CGAGACATCG AGGATTTGAC AAGGGCGACG ACGAACAGCT GCACGTGCTT 
CCACTCTACA TACTGGACGC AACCGACGAG CAGGGTGGCA AAGAAGGCTT TCATGAAAAG 
ATCAAGAATG GGTCACTCGA GGTTTTGTCG AAATTTCACA CGACGTCCAG GATAAGAAAG 
GTCCCCTTGG GGCCCTGCAA AAAGCGTGGA AGGAAAGAAA AGGTGACCGA GGAGCCGCTG 
CACTCAATTC AGCAGTCTGC AAGTTCTGAG GCCCAAACAG ATGAAGGGTA TGCTTCCCAA 
ACCATGCCAT CACCAGTGTT CAGCCCCAGC TTCGAAGTGC CACAGGCGTA TTGCAGATCT 
CCTTTCGAAG ACAAGTACAA ATCAGGGATG AGCAAAATGA AGCACGAGTC GGATCTCAGC 
CACCTGAGAT ACGAGATGCC AGCAAGTCCC TTCCGATCTC ACTCTCAGCA GGCCTTCCAC 
AGCACCAGGG ACCTCACCAT GGTGCAGTCT CCTTCCAGCG GAATGGTCGG AAACAAGGTC 
AAGAATGAGG CTGTTCCTCA AATGTCTTGC CAGTTTGCTG CGGCTCCGAG CGAGAACCTT 
GGCTTGCAGA AGCAGGACTA TGGCAGTGCT GAATCTCAGG GTCCTTGTTT AGGAGCTTCC 
GGGAGGGAGG ACACAAAGGT AGTAGTCAAA AACGAGTATG GCGGCGAGTC CTGCATCCGG 
AACACTGCGG GTAAAGAGGC GGAAATGTTC AGCAAACATC TCGGACAGTT ACAATCACCG 
ACACTTCAGA GTCCTCTAGA AAAACCTCAT CATCATGCGC GAAAAAGCAT ACAGAGTTCA 
CCCAGCCCCA TGATTCAGCC GTCCTATCTT CCACCTCAGC AAGACCTGAG TTACAGCAAC 
CATTACTTCA GCGGCAGCTA CATGCATCAG GGCAGGCAGC CTAGCCCCGG CCTGTGCACT 
CTTTCGCAAA ATCCGCTCGT GTCTGGCATT CCGGAGAGTC CCCCTGAAGA CCATCACCTC 
GCAATGAACT GCTACAGGAT GACCGACAGG TACCATGCCT ACCCAAACCC TCTTCAGCAC 
GTCGGTCAGG AAGGGCACGC TCTGGAAACT CACGGGATAG ACCACCTCCA GAACTTGGCG 
TCCGTCTCAA ACCATCACCT GCAGAACCTG GAGGTCAACC GGCCTAGCGG GGGTCACGGC 
GTGTACCCGC CCTTCATGGG GAGGCCTGCG CCGATCCAGC ACTACGACCA TCAACCCATG 
AACAACCCCA CGTTCAACCA CATCTCCCAG GCGCCTTCAC CGCAGGGGGT CATGCTTCCA 
CCCCAGTCTT ACATTGCCCA GCCCGTCTCG CCGCACCTGA CGAGGAACGT CGGCTACGGC 
GTGCCCGGGC TCTCCAGGAC CGGCGGCTTC CTCCCGTCTC CTCACGAGGC CTTCTTATCT 
CACCCCAGTC CTCCCGTCAC CCCCATGACT CCTCCCAGCA CACCGAGGCT CACGGACCTC 
AATCCCGTCA GGATCGACTC TCCGCTCCAC TTGCGAGCCT CTTACTACTC GGCCGCGACG 
AGTCCCTTCG GCGTGCCGGT CGACCCGTCC TACTCTCCGC TGGCCAGGAG CCACAGTCAG 
GGGCCCGCCT TTGCGCCATA CATGGACGGA GCGGCCTGGG CCTCCGACCG TGACTACAAC 
AGTGCGGCTC TCCAAAACTC CTTTTACGTG CCGCGCTCCC AGTGCTTCCC GACGGCAGCA 
CCGCCGCTGC CCCAGAAAGA CTATGCGTTC CGCGCGCCCC CGGGCAGCCA CTTTGCAGGA 
CCTTCTCTTC CCTCTTCGTC GCGTCTTCCG CCCGGCCTGG CCACGCCTCC GCAGCAGGAC 
CGCTCTGCCA CCGCCAGCGT GCTTCCCCCG TTCAAGAACA CGTTTCTCCC GGCGCAGTCT 
GCCCTCAACG GTTTCGAGCG TCACCTGCAG ACGTTCAAGT CGGTGATGAT GGAAGACGCC 
GCGCAGAGGA GCTCCGCCTC TGGGCTCTAC GGCGCTCCGG CCCCCACGGG CCGCCCTTCT 
CAGCGGCCAG CTAGTCACGA GGGCTTCACG AGGACGCCTC TCGAACCGCC CGTCACGGAA 
GAGTCTTCCG AGGGGATCTA CGAGGTGGAC TCGGACAACG AAAGCGCCTT CCAGGACGAA 
GAGGTTGGCG GCGTCGCCAT TGCGCTCACT CACGGGTCGG TGCTCTTCGA GTGCGCCAAG 
CACGAGCTGC ATGCGACGAC TGCTCTGCGG AGGCCCAACC GCAAGAACCC CACCCGCATC 
AGCCTCGTGT TCTACCAGCA CAAGAACCTC AACTTCCGGA ATCACGGGGA GGAGGAGTGG 
GAGAAGAAGA TGGAGGTTCG CAAGTTGGAA AGAGCCAACG GGGAGCGGCC CTCCAAGAAG 
AAGGCCAAGC TCTCCGTGGA CAGGTTGGAT GAGGGTGGCG GCTTCCCCGC GGCTCCCGCA 
GACGTCGGCC AGCCGTGGGA GGTGCCCCCC CGCGTGTCCC TGCCGTCGTT CTTCGCCATC 
AACCCTTACC AGAAGTGTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838416.1
CDS	35..5338
Translation	MMHQMHERRRGTPDENKNLQQVASAICGSGVAGKDGMAASPGNRTNLNGRAVNGGGASTSPYLPQGVPEHLGHPGIGGFPGHSEYQLPPTQKPAPPAQAGQMYEMGPHFGAFPGHPSQALAFNGHMAQHDAGRLGLPGEEGDFPTRHQMMNSRLKTLIQTRQSQKEQGPFQGLETTLGPSVGGSFVHPQADATATASRGFPPLVRSNGGCGAVSGNNNGGNNGGGNNNNNGTNNGGGNNGNGGTGGGNNNPRPLSSNEMPGDAWHDASPSSGPPPGRAGVAELPEFAKNGSSSSSQGSNNNANNNNSEAEFSQPEDQSHESEFAEGGAAETRGGGGGDRSSAPPNGMLPYGSFYGAQEAAEVRKGGEGKVPPPTTSAATTTSAADVHGGGAHYQFTSAATSMSTTTTSASVHGKPCNGPTQPSFAERQSPRGGPFSGAPTSFAPAPHLASMHQERPVPESPTQLRNEVGEAGSGGQTRGNESESTDAAAVTSQPFLSNTTTSMNTYTSIINTYNSFAASCAPSFLAEHQQPKFGGSVEDAARTGGGDASDVDGGRIAVGEFRGLDFGAPPLPHHPGGPEGPRPHYPQYGGAPAAFVPEGPYAVPYGAGNLGNLGNPFPLPPMGAGGPPFAPMSLPGADPWWERLERLRSNAKAEPPACDCYGADETPPVDKSPYYTHLGAGPTVAAIREMLEHRLNERGPALRIEKVLYSGKEGKTSQGCPVAKWIIRRSGPSEKVLAVLRHRPGHRCLSAYIVMAIVAWEGVQADMADDLYRTVTHKTVNFGFPTQRRCGTNEQRTCACQGADAENCGASFSFGCSWSMYYNGCKYARSKAVRKFKLSEQSQEQELEDKMQMLATEMAPLYARVAPESYKNQTEFENEGISCRLGLKQGRPFSGVTACVDFCAHAHKDLHNMNNGCTVVVTLTRHRGFDKGDDEQLHVLPLYILDATDEQGGKEGFHEKIKNGSLEVLSKFHTTSRIRKVPLGPCKKRGRKEKVTEEPLHSIQQSASSEAQTDEGYASQTMPSPVFSPSFEVPQAYCRSPFEDKYKSGMSKMKHESDLSHLRYEMPASPFRSHSQQAFHSTRDLTMVQSPSSGMVGNKVKNEAVPQMSCQFAAAPSENLGLQKQDYGSAESQGPCLGASGREDTKVVVKNEYGGESCIRNTAGKEAEMFSKHLGQLQSPTLQSPLEKPHHHARKSIQSSPSPMIQPSYLPPQQDLSYSNHYFSGSYMHQGRQPSPGLCTLSQNPLVSGIPESPPEDHHLAMNCYRMTDRYHAYPNPLQHVGQEGHALETHGIDHLQNLASVSNHHLQNLEVNRPSGGHGVYPPFMGRPAPIQHYDHQPMNNPTFNHISQAPSPQGVMLPPQSYIAQPVSPHLTRNVGYGVPGLSRTGGFLPSPHEAFLSHPSPPVTPMTPPSTPRLTDLNPVRIDSPLHLRASYYSAATSPFGVPVDPSYSPLARSHSQGPAFAPYMDGAAWASDRDYNSAALQNSFYVPRSQCFPTAAPPLPQKDYAFRAPPGSHFAGPSLPSSSRLPPGLATPPQQDRSATASVLPPFKNTFLPAQSALNGFERHLQTFKSVMMEDAAQRSSASGLYGAPAPTGRPSQRPASHEGFTRTPLEPPVTEESSEGIYEVDSDNESAFQDEEVGGVAIALTHGSVLFECAKHELHATTALRRPNRKNPTRISLVFYQHKNLNFRNHGEEEWEKKMEVRKLERANGERPSKKKAKLSVDRLDEGGGFPAAPADVGQPWEVPPRVSLPSFFAINPYQKCF

Target ORF information:

RefSeq Version	XM_029982556.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982556.1

ORF Insert Sequence:

ATGATGCATC AGATGCACGA AAGGCGTCGG GGTACACCAG ATGAAAATAA AAACTTGCAA 
CAGGTAGCGA GCGCCATTTG CGGAAGCGGG GTCGCCGGAA AAGACGGCAT GGCGGCCTCT 
CCGGGCAACC GAACTAATCT CAACGGGCGG GCGGTGAACG GCGGCGGCGC TTCGACGTCG 
CCCTACCTCC CGCAGGGAGT GCCGGAACAC CTCGGACATC CTGGGATCGG TGGCTTTCCC 
GGTCACAGCG AGTACCAGCT CCCGCCCACG CAAAAGCCTG CCCCGCCGGC CCAGGCCGGC 
CAGATGTACG AGATGGGGCC GCACTTCGGG GCCTTCCCGG GCCACCCGTC TCAGGCCCTG 
GCTTTCAATG GCCACATGGC ACAGCACGAC GCTGGCCGCC TGGGGCTCCC CGGCGAAGAG 
GGCGACTTCC CCACGCGCCA CCAGATGATG AACAGCCGTC TCAAGACACT GATTCAGACT 
AGGCAGAGCC AGAAGGAGCA GGGTCCCTTC CAGGGGCTCG AGACGACCCT AGGCCCCTCC 
GTCGGAGGAA GCTTTGTGCA CCCGCAGGCC GATGCGACGG CGACTGCCTC CCGGGGGTTC 
CCGCCGCTTG TGCGGAGCAA CGGTGGCTGC GGTGCCGTGA GCGGCAACAA CAACGGCGGA 
AACAATGGCG GCGGCAACAA CAACAACAAC GGCACCAACA ACGGCGGTGG TAACAACGGC 
AACGGCGGCA CGGGCGGCGG TAACAACAAT CCGAGGCCTC TGTCGTCAAA CGAGATGCCC 
GGGGATGCGT GGCACGATGC GAGCCCGTCC TCGGGTCCTC CGCCGGGCAG GGCTGGTGTC 
GCAGAGCTAC CGGAGTTTGC CAAGAACGGG TCCTCTTCCT CCTCCCAGGG AAGCAACAAC 
AACGCCAACA ACAACAACTC CGAAGCTGAG TTCTCCCAGC CTGAGGATCA GAGTCACGAG 
AGTGAGTTTG CAGAGGGTGG CGCTGCGGAA ACCAGGGGAG GTGGCGGCGG CGACAGGTCG 
AGCGCTCCCC CCAACGGAAT GTTGCCCTAC GGAAGCTTCT ATGGGGCGCA AGAGGCGGCA 
GAAGTCCGGA AGGGTGGCGA GGGCAAGGTC CCCCCGCCGA CAACGAGCGC GGCGACGACC 
ACTTCGGCGG CGGACGTTCA CGGTGGTGGT GCGCATTACC AGTTCACGTC GGCAGCGACT 
TCGATGAGCA CGACGACGAC GAGTGCCTCC GTTCACGGCA AACCCTGCAA CGGACCAACG 
CAGCCTTCGT TTGCGGAGCG GCAGTCGCCC AGGGGCGGCC CGTTCTCCGG TGCACCGACG 
TCATTCGCTC CGGCGCCCCA CTTGGCGAGT ATGCACCAAG AGCGCCCGGT CCCCGAAAGT 
CCGACGCAGC TCCGCAACGA GGTAGGCGAA GCCGGCAGCG GCGGCCAGAC GCGCGGCAAT 
GAGAGCGAAT CCACGGACGC GGCGGCAGTC ACATCGCAGC CTTTTTTATC GAATACGACC 
ACATCTATGA ATACATATAC AAGTATTATC AATACATATA ACAGCTTCGC CGCTTCGTGC 
GCGCCGTCCT TCTTGGCCGA GCACCAGCAA CCCAAGTTTG GCGGGAGCGT GGAGGATGCG 
GCTCGAACGG GGGGAGGAGA CGCATCCGAC GTAGATGGTG GTCGTATTGC CGTCGGCGAA 
TTCCGTGGAC TCGACTTTGG GGCACCCCCC CTGCCTCACC ACCCCGGTGG CCCCGAGGGC 
CCCAGGCCGC ACTACCCCCA GTACGGGGGG GCGCCTGCCG CGTTTGTGCC AGAGGGTCCT 
TACGCTGTTC CCTATGGGGC CGGGAACCTG GGGAATCTGG GGAACCCGTT CCCCTTGCCC 
CCCATGGGCG CCGGAGGTCC TCCCTTTGCG CCCATGAGCC TCCCTGGTGC CGACCCCTGG 
TGGGAGCGGC TGGAGCGGCT ACGCAGCAAC GCCAAGGCGG AGCCTCCGGC CTGCGACTGC 
TATGGCGCGG ATGAGACGCC TCCCGTGGAT AAATCCCCAT ACTATACCCA CCTTGGTGCT 
GGACCGACTG TGGCTGCTAT TAGAGAGATG CTTGAGCACA GGCTGAACGA ACGGGGCCCT 
GCGTTGCGCA TCGAGAAGGT GCTCTACTCG GGCAAGGAGG GCAAGACGAG CCAGGGCTGC 
CCGGTGGCCA AGTGGATCAT CCGGCGCAGT GGCCCTTCGG AGAAGGTGCT GGCCGTGCTG 
CGGCACCGAC CGGGCCACCG CTGCTTGTCG GCCTACATCG TGATGGCCAT CGTCGCCTGG 
GAGGGCGTCC AGGCAGACAT GGCAGACGAC CTCTACAGGA CCGTGACCCA CAAGACCGTC 
AACTTTGGCT TCCCCACTCA GCGGAGATGT GGCACCAACG AACAACGAAC CTGCGCCTGT 
CAAGGTGCCG ACGCCGAGAA CTGTGGCGCT TCCTTTTCAT TTGGCTGCTC TTGGAGCATG 
TATTACAATG GCTGCAAGTA TGCACGGAGC AAGGCTGTCC GCAAGTTCAA GCTCTCAGAA 
CAGTCCCAGG AGCAAGAGCT TGAAGACAAA ATGCAGATGC TCGCCACTGA AATGGCACCG 
CTGTATGCAA GGGTGGCTCC GGAATCCTAC AAAAACCAGA CCGAGTTTGA GAACGAAGGG 
ATATCCTGCA GACTAGGGTT GAAGCAGGGA AGACCCTTTT CTGGCGTCAC GGCGTGTGTC 
GACTTCTGTG CCCATGCGCA CAAGGACCTA CACAACATGA ATAACGGCTG TACAGTTGTT 
GTAACCCTAA CGAGACATCG AGGATTTGAC AAGGGCGACG ACGAACAGCT GCACGTGCTT 
CCACTCTACA TACTGGACGC AACCGACGAG CAGGGTGGCA AAGAAGGCTT TCATGAAAAG 
ATCAAGAATG GGTCACTCGA GGTTTTGTCG AAATTTCACA CGACGTCCAG GATAAGAAAG 
GTCCCCTTGG GGCCCTGCAA AAAGCGTGGA AGGAAAGAAA AGGTGACCGA GGAGCCGCTG 
CACTCAATTC AGCAGTCTGC AAGTTCTGAG GCCCAAACAG ATGAAGGGTA TGCTTCCCAA 
ACCATGCCAT CACCAGTGTT CAGCCCCAGC TTCGAAGTGC CACAGGCGTA TTGCAGATCT 
CCTTTCGAAG ACAAGTACAA ATCAGGGATG AGCAAAATGA AGCACGAGTC GGATCTCAGC 
CACCTGAGAT ACGAGATGCC AGCAAGTCCC TTCCGATCTC ACTCTCAGCA GGCCTTCCAC 
AGCACCAGGG ACCTCACCAT GGTGCAGTCT CCTTCCAGCG GAATGGTCGG AAACAAGGTC 
AAGAATGAGG CTGTTCCTCA AATGTCTTGC CAGTTTGCTG CGGCTCCGAG CGAGAACCTT 
GGCTTGCAGA AGCAGGACTA TGGCAGTGCT GAATCTCAGG GTCCTTGTTT AGGAGCTTCC 
GGGAGGGAGG ACACAAAGGT AGTAGTCAAA AACGAGTATG GCGGCGAGTC CTGCATCCGG 
AACACTGCGG GTAAAGAGGC GGAAATGTTC AGCAAACATC TCGGACAGTT ACAATCACCG 
ACACTTCAGA GTCCTCTAGA AAAACCTCAT CATCATGCGC GAAAAAGCAT ACAGAGTTCA 
CCCAGCCCCA TGATTCAGCC GTCCTATCTT CCACCTCAGC AAGACCTGAG TTACAGCAAC 
CATTACTTCA GCGGCAGCTA CATGCATCAG GGCAGGCAGC CTAGCCCCGG CCTGTGCACT 
CTTTCGCAAA ATCCGCTCGT GTCTGGCATT CCGGAGAGTC CCCCTGAAGA CCATCACCTC 
GCAATGAACT GCTACAGGAT GACCGACAGG TACCATGCCT ACCCAAACCC TCTTCAGCAC 
GTCGGTCAGG AAGGGCACGC TCTGGAAACT CACGGGATAG ACCACCTCCA GAACTTGGCG 
TCCGTCTCAA ACCATCACCT GCAGAACCTG GAGGTCAACC GGCCTAGCGG GGGTCACGGC 
GTGTACCCGC CCTTCATGGG GAGGCCTGCG CCGATCCAGC ACTACGACCA TCAACCCATG 
AACAACCCCA CGTTCAACCA CATCTCCCAG GCGCCTTCAC CGCAGGGGGT CATGCTTCCA 
CCCCAGTCTT ACATTGCCCA GCCCGTCTCG CCGCACCTGA CGAGGAACGT CGGCTACGGC 
GTGCCCGGGC TCTCCAGGAC CGGCGGCTTC CTCCCGTCTC CTCACGAGGC CTTCTTATCT 
CACCCCAGTC CTCCCGTCAC CCCCATGACT CCTCCCAGCA CACCGAGGCT CACGGACCTC 
AATCCCGTCA GGATCGACTC TCCGCTCCAC TTGCGAGCCT CTTACTACTC GGCCGCGACG 
AGTCCCTTCG GCGTGCCGGT CGACCCGTCC TACTCTCCGC TGGCCAGGAG CCACAGTCAG 
GGGCCCGCCT TTGCGCCATA CATGGACGGA GCGGCCTGGG CCTCCGACCG TGACTACAAC 
AGTGCGGCTC TCCAAAACTC CTTTTACGTG CCGCGCTCCC AGTGCTTCCC GACGGCAGCA 
CCGCCGCTGC CCCAGAAAGA CTATGCGTTC CGCGCGCCCC CGGGCAGCCA CTTTGCAGGA 
CCTTCTCTTC CCTCTTCGTC GCGTCTTCCG CCCGGCCTGG CCACGCCTCC GCAGCAGGAC 
CGCTCTGCCA CCGCCAGCGT GCTTCCCCCG TTCAAGAACA CGTTTCTCCC GGCGCAGTCT 
GCCCTCAACG GTTTCGAGCG TCACCTGCAG ACGTTCAAGT CGGTGATGAT GGAAGACGCC 
GCGCAGAGGA GCTCCGCCTC TGGGCTCTAC GGCGCTCCGG CCCCCACGGG CCGCCCTTCT 
CAGCGGCCAG CTAGTCACGA GGGCTTCACG AGGACGCCTC TCGAACCGCC CGTCACGGAA 
GAGTCTTCCG AGGGGATCTA CGAGGTGGAC TCGGACAACG AAAGCGCCTT CCAGGACGAA 
GAGGTTGGCG GCGTCGCCAT TGCGCTCACT CACGGGTCGG TGCTCTTCGA GTGCGCCAAG 
CACGAGCTGC ATGCGACGAC TGCTCTGCGG AGGCCCAACC GCAAGAACCC CACCCGCATC 
AGCCTCGTGT TCTACCAGCA CAAGAACCTC AACTTCCGGA ATCACGGGGA GGAGGAGTGG 
GAGAAGAAGA TGGAGGTTCG CAAGTTGGAA AGAGCCAACG GGGAGCGGCC CTCCAAGAAG 
AAGGCCAAGC TCTCCGTGGA CAGGTTGGAT GAGGGTGGCG GCTTCCCCGC GGCTCCCGCA 
GACGTCGGCC AGCCGTGGGA GGTGCCCCCC CGCGTGTCCC TGCCGTCGTT CTTCGCCATC 
AACCCTTACC AGAAGTGTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8042599 ( XM_029982550.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838410.1 Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X1, mRNA.

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CloneID	OIa25172
Clone ID Related Accession (Same CDS sequence)	XM_029982550.1
Accession Version	XM_029982550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	uncharacterized protein LOC8042599 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919951.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA GGCAGCCCCT GCACGGAGGC AGCCCCTTCG AGCCCGGCGC CCAGGGACCC 
GCGGGGCCGG GCCCCCCGAC CCTCCTGGCC CAGGACGGCT GGATGGTGGC CGGGGGGCCG 
CCGCCGCCCT ACCCGATCCT GCCGCAGGAG TTTGCGCCGC AGGGACCCCA CTTCGGCTCC 
TTCGACCCGG TGGGCCAGTT CAACAAGGTG ACCCTGCCGC CTTCCTTCCC GTTCCCGGGC 
CTCGGGTTCC CGGCGCGGGA GCCAGACGAG CCGGCCAACG GCGGCAAGGA GGAGGGCCTC 
AGCGCGCGCC TGCTCGACAA GACGAGTAGC AGCGACGGGT CCCCGCGGTC GGGCACGCCC 
GCGGGCAACG TGGGCTACCG GCCCTGGGAG CAGGAGGGCT ACGAGAGGCC CCCCAGCTCG 
CCGCCCAAGG ACCGGCCCTT CAGCAACGGC GGCGCCGACA AGAGCGGCTC GCCGGGGCTC 
CAGTCCCAGG ACAGCTCTCA GTCTAGCGAC CGGATGCCCA CCCCCGTAGA CAAGATGGCG 
GCGCAGCACC CGGCGTCCCA GTTCGGCAGT CCGATGCAGC ATCATCCCTT CGCCGACTTC 
CCCCCGCACC ACGCGGTGTT CTTTGCGCCC GACAAGGGCA ACGCCGAAGA GCCGTCCTTC 
CAGGTGCCCT CCGTGTCGTC CACCACGACT AGGCACACGC TGCTGCCCAT GGTGCCGCTC 
CAGGAGCCCT GGGGAGACCA GCCGCCCATG GCCAGGAACG GCCTGGTGAT GCACCACCAG 
CAGCCGCAGC AGCAGCCGCC TCACCACCAC CACCAGGGCG GGCCGCCCCA GCAGCAGCAG 
CAACAGCAGC AGCAGCCCGG CACCCCGGAC GGCATGGAGA AGGGCACCAA GAAGAAACGC 
AAACGCTGCG GCGAGTGCCC TGGTTGCCTC AAGAAGGATA ACTGCGGAGA GTGCGGGCCG 
TGCAGGAGTG TGCGCAGTCA CCAGATCTGC AAGATGCGGA AGTGCGATCA GCTCAAGACC 
AAAAAGGAGA AAGAGCGCTA TGAGAAGATG ATGCATCAGA TGCACGAAAG GCGTCGGGGT 
ACACCAGATG AAAATAAAAA CTTGCAACAG GTAGCGAGCG CCATTTGCGG AAGCGGGGTC 
GCCGGAAAAG ACGGCATGGC GGCCTCTCCG GGCAACCGAA CTAATCTCAA CGGGCGGGCG 
GTGAACGGCG GCGGCGCTTC GACGTCGCCC TACCTCCCGC AGGGAGTGCC GGAACACCTC 
GGACATCCTG GGATCGGTGG CTTTCCCGGT CACAGCGAGT ACCAGCTCCC GCCCACGCAA 
AAGCCTGCCC CGCCGGCCCA GGCCGGCCAG ATGTACGAGA TGGGGCCGCA CTTCGGGGCC 
TTCCCGGGCC ACCCGTCTCA GGCCCTGGCT TTCAATGGCC ACATGGCACA GCACGACGCT 
GGCCGCCTGG GGCTCCCCGG CGAAGAGGGC GACTTCCCCA CGCGCCACCA GATGATGAAC 
AGCCGTCTCA AGACACTGAT TCAGACTAGG CAGAGCCAGA AGGAGCAGGG TCCCTTCCAG 
GGGCTCGAGA CGACCCTAGG CCCCTCCGTC GGAGGAAGCT TTGTGCACCC GCAGGCCGAT 
GCGACGGCGA CTGCCTCCCG GGGGTTCCCG CCGCTTGTGC GGAGCAACGG TGGCTGCGGT 
GCCGTGAGCG GCAACAACAA CGGCGGAAAC AATGGCGGCG GCAACAACAA CAACAACGGC 
ACCAACAACG GCGGTGGTAA CAACGGCAAC GGCGGCACGG GCGGCGGTAA CAACAATCCG 
AGGCCTCTGT CGTCAAACGA GATGCCCGGG GATGCGTGGC ACGATGCGAG CCCGTCCTCG 
GGTCCTCCGC CGGGCAGGGC TGGTGTCGCA GAGCTACCGG AGTTTGCCAA GAACGGGTCC 
TCTTCCTCCT CCCAGGGAAG CAACAACAAC GCCAACAACA ACAACTCCGA AGCTGAGTTC 
TCCCAGCCTG AGGATCAGAG TCACGAGAGT GAGTTTGCAG AGGGTGGCGC TGCGGAAACC 
AGGGGAGGTG GCGGCGGCGA CAGGTCGAGC GCTCCCCCCA ACGGAATGTT GCCCTACGGA 
AGCTTCTATG GGGCGCAAGA GGCGGCAGAA GTCCGGAAGG GTGGCGAGGG CAAGGTCCCC 
CCGCCGACAA CGAGCGCGGC GACGACCACT TCGGCGGCGG ACGTTCACGG TGGTGGTGCG 
CATTACCAGT TCACGTCGGC AGCGACTTCG ATGAGCACGA CGACGACGAG TGCCTCCGTT 
CACGGCAAAC CCTGCAACGG ACCAACGCAG CCTTCGTTTG CGGAGCGGCA GTCGCCCAGG 
GGCGGCCCGT TCTCCGGTGC ACCGACGTCA TTCGCTCCGG CGCCCCACTT GGCGAGTATG 
CACCAAGAGC GCCCGGTCCC CGAAAGTCCG ACGCAGCTCC GCAACGAGGT AGGCGAAGCC 
GGCAGCGGCG GCCAGACGCG CGGCAATGAG AGCGAATCCA CGGACGCGGC GGCAGTCACA 
TCGCAGCCTT TTTTATCGAA TACGACCACA TCTATGAATA CATATACAAG TATTATCAAT 
ACATATAACA GCTTCGCCGC TTCGTGCGCG CCGTCCTTCT TGGCCGAGCA CCAGCAACCC 
AAGTTTGGCG GGAGCGTGGA GGATGCGGCT CGAACGGGGG GAGGAGACGC ATCCGACGTA 
GATGGTGGTC GTATTGCCGT CGGCGAATTC CGTGGACTCG ACTTTGGGGC ACCCCCCCTG 
CCTCACCACC CCGGTGGCCC CGAGGGCCCC AGGCCGCACT ACCCCCAGTA CGGGGGGGCG 
CCTGCCGCGT TTGTGCCAGA GGGTCCTTAC GCTGTTCCCT ATGGGGCCGG GAACCTGGGG 
AATCTGGGGA ACCCGTTCCC CTTGCCCCCC ATGGGCGCCG GAGGTCCTCC CTTTGCGCCC 
ATGAGCCTCC CTGGTGCCGA CCCCTGGTGG GAGCGGCTGG AGCGGCTACG CAGCAACGCC 
AAGGCGGAGC CTCCGGCCTG CGACTGCTAT GGCGCGGATG AGACGCCTCC CGTGGATAAA 
TCCCCATACT ATACCCACCT TGGTGCTGGA CCGACTGTGG CTGCTATTAG AGAGATGCTT 
GAGCACAGGC TGAACGAACG GGGCCCTGCG TTGCGCATCG AGAAGGTGCT CTACTCGGGC 
AAGGAGGGCA AGACGAGCCA GGGCTGCCCG GTGGCCAAGT GGATCATCCG GCGCAGTGGC 
CCTTCGGAGA AGGTGCTGGC CGTGCTGCGG CACCGACCGG GCCACCGCTG CTTGTCGGCC 
TACATCGTGA TGGCCATCGT CGCCTGGGAG GGCGTCCAGG CAGACATGGC AGACGACCTC 
TACAGGACCG TGACCCACAA GACCGTCAAC TTTGGCTTCC CCACTCAGCG GAGATGTGGC 
ACCAACGAAC AACGAACCTG CGCCTGTCAA GGTGCCGACG CCGAGAACTG TGGCGCTTCC 
TTTTCATTTG GCTGCTCTTG GAGCATGTAT TACAATGGCT GCAAGTATGC ACGGAGCAAG 
GCTGTCCGCA AGTTCAAGCT CTCAGAACAG TCCCAGGAGC AAGAGCTTGA AGACAAAATG 
CAGATGCTCG CCACTGAAAT GGCACCGCTG TATGCAAGGG TGGCTCCGGA ATCCTACAAA 
AACCAGACCG AGTTTGAGAA CGAAGGGATA TCCTGCAGAC TAGGGTTGAA GCAGGGAAGA 
CCCTTTTCTG GCGTCACGGC GTGTGTCGAC TTCTGTGCCC ATGCGCACAA GGACCTACAC 
AACATGAATA ACGGCTGTAC AGTTGTTGTA ACCCTAACGA GACATCGAGG ATTTGACAAG 
GGCGACGACG AACAGCTGCA CGTGCTTCCA CTCTACATAC TGGACGCAAC CGACGAGCAG 
GGTGGCAAAG AAGGCTTTCA TGAAAAGATC AAGAATGGGT CACTCGAGGT TTTGTCGAAA 
TTTCACACGA CGTCCAGGAT AAGAAAGGTC CCCTTGGGGC CCTGCAAAAA GCGTGGAAGG 
AAAGAAAAGG TGACCGAGGA GCCGCTGCAC TCAATTCAGC AGTCTGCAAG TTCTGAGGCC 
CAAACAGATG AAGGGTATGC TTCCCAAACC ATGCCATCAC CAGTGTTCAG CCCCAGCTTC 
GAAGTGCCAC AGGCGTATTG CAGATCTCCT TTCGAAGACA AGTACAAATC AGGGATGAGC 
AAAATGAAGC ACGAGTCGGA TCTCAGCCAC CTGAGATACG AGATGCCAGC AAGTCCCTTC 
CGATCTCACT CTCAGCAGGC CTTCCACAGC ACCAGGGACC TCACCATGGT GCAGTCTCCT 
TCCAGCGGAA TGGTCGGAAA CAAGGTCAAG AATGAGGCTG TTCCTCAAAT GTCTTGCCAG 
TTTGCTGCGG CTCCGAGCGA GAACCTTGGC TTGCAGAAGC AGGACTATGG CAGTGCTGAA 
TCTCAGGGTC CTTGTTTAGG AGCTTCCGGG AGGGAGGACA CAAAGGTAGT AGTCAAAAAC 
GAGTATGGCG GCGAGTCCTG CATCCGGAAC ACTGCGGGTA AAGAGGCGGA AATGTTCAGC 
AAACATCTCG GACAGTTACA ATCACCGACA CTTCAGAGTC CTCTAGAAAA ACCTCATCAT 
CATGCGCGAA AAAGCATACA GAGTTCACCC AGCCCCATGA TTCAGCCGTC CTATCTTCCA 
CCTCAGCAAG ACCTGAGTTA CAGCAACCAT TACTTCAGCG GCAGCTACAT GCATCAGGGC 
AGGCAGCCTA GCCCCGGCCT GTGCACTCTT TCGCAAAATC CGCTCGTGTC TGGCATTCCG 
GAGAGTCCCC CTGAAGACCA TCACCTCGCA ATGAACTGCT ACAGGATGAC CGACAGGTAC 
CATGCCTACC CAAACCCTCT TCAGCACGTC GGTCAGGAAG GGCACGCTCT GGAAACTCAC 
GGGATAGACC ACCTCCAGAA CTTGGCGTCC GTCTCAAACC ATCACCTGCA GAACCTGGAG 
GTCAACCGGC CTAGCGGGGG TCACGGCGTG TACCCGCCCT TCATGGGGAG GCCTGCGCCG 
ATCCAGCACT ACGACCATCA ACCCATGAAC AACCCCACGT TCAACCACAT CTCCCAGGCG 
CCTTCACCGC AGGGGGTCAT GCTTCCACCC CAGTCTTACA TTGCCCAGCC CGTCTCGCCG 
CACCTGACGA GGAACGTCGG CTACGGCGTG CCCGGGCTCT CCAGGACCGG CGGCTTCCTC 
CCGTCTCCTC ACGAGGCCTT CTTATCTCAC CCCAGTCCTC CCGTCACCCC CATGACTCCT 
CCCAGCACAC CGAGGCTCAC GGACCTCAAT CCCGTCAGGA TCGACTCTCC GCTCCACTTG 
CGAGCCTCTT ACTACTCGGC CGCGACGAGT CCCTTCGGCG TGCCGGTCGA CCCGTCCTAC 
TCTCCGCTGG CCAGGAGCCA CAGTCAGGGG CCCGCCTTTG CGCCATACAT GGACGGAGCG 
GCCTGGGCCT CCGACCGTGA CTACAACAGT GCGGCTCTCC AAAACTCCTT TTACGTGCCG 
CGCTCCCAGT GCTTCCCGAC GGCAGCACCG CCGCTGCCCC AGAAAGACTA TGCGTTCCGC 
GCGCCCCCGG GCAGCCACTT TGCAGGACCT TCTCTTCCCT CTTCGTCGCG TCTTCCGCCC 
GGCCTGGCCA CGCCTCCGCA GCAGGACCGC TCTGCCACCG CCAGCGTGCT TCCCCCGTTC 
AAGAACACGT TTCTCCCGGC GCAGTCTGCC CTCAACGGTT TCGAGCGTCA CCTGCAGACG 
TTCAAGTCGG TGATGATGGA AGACGCCGCG CAGAGGAGCT CCGCCTCTGG GCTCTACGGC 
GCTCCGGCCC CCACGGGCCG CCCTTCTCAG CGGCCAGCTA GTCACGAGGG CTTCACGAGG 
ACGCCTCTCG AACCGCCCGT CACGGAAGAG TCTTCCGAGG GGATCTACGA GGTGGACTCG 
GACAACGAAA GCGCCTTCCA GGACGAAGAG GTTGGCGGCG TCGCCATTGC GCTCACTCAC 
GGGTCGGTGC TCTTCGAGTG CGCCAAGCAC GAGCTGCATG CGACGACTGC TCTGCGGAGG 
CCCAACCGCA AGAACCCCAC CCGCATCAGC CTCGTGTTCT ACCAGCACAA GAACCTCAAC 
TTCCGGAATC ACGGGGAGGA GGAGTGGGAG AAGAAGATGG AGGTTCGCAA GTTGGAAAGA 
GCCAACGGGG AGCGGCCCTC CAAGAAGAAG GCCAAGCTCT CCGTGGACAG GTTGGATGAG 
GGTGGCGGCT TCCCCGCGGC TCCCGCAGAC GTCGGCCAGC CGTGGGAGGT GCCCCCCCGC 
GTGTCCCTGC CGTCGTTCTT CGCCATCAAC CCTTACCAGA AGTGTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838410.1
CDS	313..6663
Translation	MSERQPLHGGSPFEPGAQGPAGPGPPTLLAQDGWMVAGGPPPPYPILPQEFAPQGPHFGSFDPVGQFNKVTLPPSFPFPGLGFPAREPDEPANGGKEEGLSARLLDKTSSSDGSPRSGTPAGNVGYRPWEQEGYERPPSSPPKDRPFSNGGADKSGSPGLQSQDSSQSSDRMPTPVDKMAAQHPASQFGSPMQHHPFADFPPHHAVFFAPDKGNAEEPSFQVPSVSSTTTRHTLLPMVPLQEPWGDQPPMARNGLVMHHQQPQQQPPHHHHQGGPPQQQQQQQQQPGTPDGMEKGTKKKRKRCGECPGCLKKDNCGECGPCRSVRSHQICKMRKCDQLKTKKEKERYEKMMHQMHERRRGTPDENKNLQQVASAICGSGVAGKDGMAASPGNRTNLNGRAVNGGGASTSPYLPQGVPEHLGHPGIGGFPGHSEYQLPPTQKPAPPAQAGQMYEMGPHFGAFPGHPSQALAFNGHMAQHDAGRLGLPGEEGDFPTRHQMMNSRLKTLIQTRQSQKEQGPFQGLETTLGPSVGGSFVHPQADATATASRGFPPLVRSNGGCGAVSGNNNGGNNGGGNNNNNGTNNGGGNNGNGGTGGGNNNPRPLSSNEMPGDAWHDASPSSGPPPGRAGVAELPEFAKNGSSSSSQGSNNNANNNNSEAEFSQPEDQSHESEFAEGGAAETRGGGGGDRSSAPPNGMLPYGSFYGAQEAAEVRKGGEGKVPPPTTSAATTTSAADVHGGGAHYQFTSAATSMSTTTTSASVHGKPCNGPTQPSFAERQSPRGGPFSGAPTSFAPAPHLASMHQERPVPESPTQLRNEVGEAGSGGQTRGNESESTDAAAVTSQPFLSNTTTSMNTYTSIINTYNSFAASCAPSFLAEHQQPKFGGSVEDAARTGGGDASDVDGGRIAVGEFRGLDFGAPPLPHHPGGPEGPRPHYPQYGGAPAAFVPEGPYAVPYGAGNLGNLGNPFPLPPMGAGGPPFAPMSLPGADPWWERLERLRSNAKAEPPACDCYGADETPPVDKSPYYTHLGAGPTVAAIREMLEHRLNERGPALRIEKVLYSGKEGKTSQGCPVAKWIIRRSGPSEKVLAVLRHRPGHRCLSAYIVMAIVAWEGVQADMADDLYRTVTHKTVNFGFPTQRRCGTNEQRTCACQGADAENCGASFSFGCSWSMYYNGCKYARSKAVRKFKLSEQSQEQELEDKMQMLATEMAPLYARVAPESYKNQTEFENEGISCRLGLKQGRPFSGVTACVDFCAHAHKDLHNMNNGCTVVVTLTRHRGFDKGDDEQLHVLPLYILDATDEQGGKEGFHEKIKNGSLEVLSKFHTTSRIRKVPLGPCKKRGRKEKVTEEPLHSIQQSASSEAQTDEGYASQTMPSPVFSPSFEVPQAYCRSPFEDKYKSGMSKMKHESDLSHLRYEMPASPFRSHSQQAFHSTRDLTMVQSPSSGMVGNKVKNEAVPQMSCQFAAAPSENLGLQKQDYGSAESQGPCLGASGREDTKVVVKNEYGGESCIRNTAGKEAEMFSKHLGQLQSPTLQSPLEKPHHHARKSIQSSPSPMIQPSYLPPQQDLSYSNHYFSGSYMHQGRQPSPGLCTLSQNPLVSGIPESPPEDHHLAMNCYRMTDRYHAYPNPLQHVGQEGHALETHGIDHLQNLASVSNHHLQNLEVNRPSGGHGVYPPFMGRPAPIQHYDHQPMNNPTFNHISQAPSPQGVMLPPQSYIAQPVSPHLTRNVGYGVPGLSRTGGFLPSPHEAFLSHPSPPVTPMTPPSTPRLTDLNPVRIDSPLHLRASYYSAATSPFGVPVDPSYSPLARSHSQGPAFAPYMDGAAWASDRDYNSAALQNSFYVPRSQCFPTAAPPLPQKDYAFRAPPGSHFAGPSLPSSSRLPPGLATPPQQDRSATASVLPPFKNTFLPAQSALNGFERHLQTFKSVMMEDAAQRSSASGLYGAPAPTGRPSQRPASHEGFTRTPLEPPVTEESSEGIYEVDSDNESAFQDEEVGGVAIALTHGSVLFECAKHELHATTALRRPNRKNPTRISLVFYQHKNLNFRNHGEEEWEKKMEVRKLERANGERPSKKKAKLSVDRLDEGGGFPAAPADVGQPWEVPPRVSLPSFFAINPYQKCF

Target ORF information:

RefSeq Version	XM_029982550.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis uncharacterized LOC8042599 (LOC8042599), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982550.1

ORF Insert Sequence:

ATGAGCGAGA GGCAGCCCCT GCACGGAGGC AGCCCCTTCG AGCCCGGCGC CCAGGGACCC 
GCGGGGCCGG GCCCCCCGAC CCTCCTGGCC CAGGACGGCT GGATGGTGGC CGGGGGGCCG 
CCGCCGCCCT ACCCGATCCT GCCGCAGGAG TTTGCGCCGC AGGGACCCCA CTTCGGCTCC 
TTCGACCCGG TGGGCCAGTT CAACAAGGTG ACCCTGCCGC CTTCCTTCCC GTTCCCGGGC 
CTCGGGTTCC CGGCGCGGGA GCCAGACGAG CCGGCCAACG GCGGCAAGGA GGAGGGCCTC 
AGCGCGCGCC TGCTCGACAA GACGAGTAGC AGCGACGGGT CCCCGCGGTC GGGCACGCCC 
GCGGGCAACG TGGGCTACCG GCCCTGGGAG CAGGAGGGCT ACGAGAGGCC CCCCAGCTCG 
CCGCCCAAGG ACCGGCCCTT CAGCAACGGC GGCGCCGACA AGAGCGGCTC GCCGGGGCTC 
CAGTCCCAGG ACAGCTCTCA GTCTAGCGAC CGGATGCCCA CCCCCGTAGA CAAGATGGCG 
GCGCAGCACC CGGCGTCCCA GTTCGGCAGT CCGATGCAGC ATCATCCCTT CGCCGACTTC 
CCCCCGCACC ACGCGGTGTT CTTTGCGCCC GACAAGGGCA ACGCCGAAGA GCCGTCCTTC 
CAGGTGCCCT CCGTGTCGTC CACCACGACT AGGCACACGC TGCTGCCCAT GGTGCCGCTC 
CAGGAGCCCT GGGGAGACCA GCCGCCCATG GCCAGGAACG GCCTGGTGAT GCACCACCAG 
CAGCCGCAGC AGCAGCCGCC TCACCACCAC CACCAGGGCG GGCCGCCCCA GCAGCAGCAG 
CAACAGCAGC AGCAGCCCGG CACCCCGGAC GGCATGGAGA AGGGCACCAA GAAGAAACGC 
AAACGCTGCG GCGAGTGCCC TGGTTGCCTC AAGAAGGATA ACTGCGGAGA GTGCGGGCCG 
TGCAGGAGTG TGCGCAGTCA CCAGATCTGC AAGATGCGGA AGTGCGATCA GCTCAAGACC 
AAAAAGGAGA AAGAGCGCTA TGAGAAGATG ATGCATCAGA TGCACGAAAG GCGTCGGGGT 
ACACCAGATG AAAATAAAAA CTTGCAACAG GTAGCGAGCG CCATTTGCGG AAGCGGGGTC 
GCCGGAAAAG ACGGCATGGC GGCCTCTCCG GGCAACCGAA CTAATCTCAA CGGGCGGGCG 
GTGAACGGCG GCGGCGCTTC GACGTCGCCC TACCTCCCGC AGGGAGTGCC GGAACACCTC 
GGACATCCTG GGATCGGTGG CTTTCCCGGT CACAGCGAGT ACCAGCTCCC GCCCACGCAA 
AAGCCTGCCC CGCCGGCCCA GGCCGGCCAG ATGTACGAGA TGGGGCCGCA CTTCGGGGCC 
TTCCCGGGCC ACCCGTCTCA GGCCCTGGCT TTCAATGGCC ACATGGCACA GCACGACGCT 
GGCCGCCTGG GGCTCCCCGG CGAAGAGGGC GACTTCCCCA CGCGCCACCA GATGATGAAC 
AGCCGTCTCA AGACACTGAT TCAGACTAGG CAGAGCCAGA AGGAGCAGGG TCCCTTCCAG 
GGGCTCGAGA CGACCCTAGG CCCCTCCGTC GGAGGAAGCT TTGTGCACCC GCAGGCCGAT 
GCGACGGCGA CTGCCTCCCG GGGGTTCCCG CCGCTTGTGC GGAGCAACGG TGGCTGCGGT 
GCCGTGAGCG GCAACAACAA CGGCGGAAAC AATGGCGGCG GCAACAACAA CAACAACGGC 
ACCAACAACG GCGGTGGTAA CAACGGCAAC GGCGGCACGG GCGGCGGTAA CAACAATCCG 
AGGCCTCTGT CGTCAAACGA GATGCCCGGG GATGCGTGGC ACGATGCGAG CCCGTCCTCG 
GGTCCTCCGC CGGGCAGGGC TGGTGTCGCA GAGCTACCGG AGTTTGCCAA GAACGGGTCC 
TCTTCCTCCT CCCAGGGAAG CAACAACAAC GCCAACAACA ACAACTCCGA AGCTGAGTTC 
TCCCAGCCTG AGGATCAGAG TCACGAGAGT GAGTTTGCAG AGGGTGGCGC TGCGGAAACC 
AGGGGAGGTG GCGGCGGCGA CAGGTCGAGC GCTCCCCCCA ACGGAATGTT GCCCTACGGA 
AGCTTCTATG GGGCGCAAGA GGCGGCAGAA GTCCGGAAGG GTGGCGAGGG CAAGGTCCCC 
CCGCCGACAA CGAGCGCGGC GACGACCACT TCGGCGGCGG ACGTTCACGG TGGTGGTGCG 
CATTACCAGT TCACGTCGGC AGCGACTTCG ATGAGCACGA CGACGACGAG TGCCTCCGTT 
CACGGCAAAC CCTGCAACGG ACCAACGCAG CCTTCGTTTG CGGAGCGGCA GTCGCCCAGG 
GGCGGCCCGT TCTCCGGTGC ACCGACGTCA TTCGCTCCGG CGCCCCACTT GGCGAGTATG 
CACCAAGAGC GCCCGGTCCC CGAAAGTCCG ACGCAGCTCC GCAACGAGGT AGGCGAAGCC 
GGCAGCGGCG GCCAGACGCG CGGCAATGAG AGCGAATCCA CGGACGCGGC GGCAGTCACA 
TCGCAGCCTT TTTTATCGAA TACGACCACA TCTATGAATA CATATACAAG TATTATCAAT 
ACATATAACA GCTTCGCCGC TTCGTGCGCG CCGTCCTTCT TGGCCGAGCA CCAGCAACCC 
AAGTTTGGCG GGAGCGTGGA GGATGCGGCT CGAACGGGGG GAGGAGACGC ATCCGACGTA 
GATGGTGGTC GTATTGCCGT CGGCGAATTC CGTGGACTCG ACTTTGGGGC ACCCCCCCTG 
CCTCACCACC CCGGTGGCCC CGAGGGCCCC AGGCCGCACT ACCCCCAGTA CGGGGGGGCG 
CCTGCCGCGT TTGTGCCAGA GGGTCCTTAC GCTGTTCCCT ATGGGGCCGG GAACCTGGGG 
AATCTGGGGA ACCCGTTCCC CTTGCCCCCC ATGGGCGCCG GAGGTCCTCC CTTTGCGCCC 
ATGAGCCTCC CTGGTGCCGA CCCCTGGTGG GAGCGGCTGG AGCGGCTACG CAGCAACGCC 
AAGGCGGAGC CTCCGGCCTG CGACTGCTAT GGCGCGGATG AGACGCCTCC CGTGGATAAA 
TCCCCATACT ATACCCACCT TGGTGCTGGA CCGACTGTGG CTGCTATTAG AGAGATGCTT 
GAGCACAGGC TGAACGAACG GGGCCCTGCG TTGCGCATCG AGAAGGTGCT CTACTCGGGC 
AAGGAGGGCA AGACGAGCCA GGGCTGCCCG GTGGCCAAGT GGATCATCCG GCGCAGTGGC 
CCTTCGGAGA AGGTGCTGGC CGTGCTGCGG CACCGACCGG GCCACCGCTG CTTGTCGGCC 
TACATCGTGA TGGCCATCGT CGCCTGGGAG GGCGTCCAGG CAGACATGGC AGACGACCTC 
TACAGGACCG TGACCCACAA GACCGTCAAC TTTGGCTTCC CCACTCAGCG GAGATGTGGC 
ACCAACGAAC AACGAACCTG CGCCTGTCAA GGTGCCGACG CCGAGAACTG TGGCGCTTCC 
TTTTCATTTG GCTGCTCTTG GAGCATGTAT TACAATGGCT GCAAGTATGC ACGGAGCAAG 
GCTGTCCGCA AGTTCAAGCT CTCAGAACAG TCCCAGGAGC AAGAGCTTGA AGACAAAATG 
CAGATGCTCG CCACTGAAAT GGCACCGCTG TATGCAAGGG TGGCTCCGGA ATCCTACAAA 
AACCAGACCG AGTTTGAGAA CGAAGGGATA TCCTGCAGAC TAGGGTTGAA GCAGGGAAGA 
CCCTTTTCTG GCGTCACGGC GTGTGTCGAC TTCTGTGCCC ATGCGCACAA GGACCTACAC 
AACATGAATA ACGGCTGTAC AGTTGTTGTA ACCCTAACGA GACATCGAGG ATTTGACAAG 
GGCGACGACG AACAGCTGCA CGTGCTTCCA CTCTACATAC TGGACGCAAC CGACGAGCAG 
GGTGGCAAAG AAGGCTTTCA TGAAAAGATC AAGAATGGGT CACTCGAGGT TTTGTCGAAA 
TTTCACACGA CGTCCAGGAT AAGAAAGGTC CCCTTGGGGC CCTGCAAAAA GCGTGGAAGG 
AAAGAAAAGG TGACCGAGGA GCCGCTGCAC TCAATTCAGC AGTCTGCAAG TTCTGAGGCC 
CAAACAGATG AAGGGTATGC TTCCCAAACC ATGCCATCAC CAGTGTTCAG CCCCAGCTTC 
GAAGTGCCAC AGGCGTATTG CAGATCTCCT TTCGAAGACA AGTACAAATC AGGGATGAGC 
AAAATGAAGC ACGAGTCGGA TCTCAGCCAC CTGAGATACG AGATGCCAGC AAGTCCCTTC 
CGATCTCACT CTCAGCAGGC CTTCCACAGC ACCAGGGACC TCACCATGGT GCAGTCTCCT 
TCCAGCGGAA TGGTCGGAAA CAAGGTCAAG AATGAGGCTG TTCCTCAAAT GTCTTGCCAG 
TTTGCTGCGG CTCCGAGCGA GAACCTTGGC TTGCAGAAGC AGGACTATGG CAGTGCTGAA 
TCTCAGGGTC CTTGTTTAGG AGCTTCCGGG AGGGAGGACA CAAAGGTAGT AGTCAAAAAC 
GAGTATGGCG GCGAGTCCTG CATCCGGAAC ACTGCGGGTA AAGAGGCGGA AATGTTCAGC 
AAACATCTCG GACAGTTACA ATCACCGACA CTTCAGAGTC CTCTAGAAAA ACCTCATCAT 
CATGCGCGAA AAAGCATACA GAGTTCACCC AGCCCCATGA TTCAGCCGTC CTATCTTCCA 
CCTCAGCAAG ACCTGAGTTA CAGCAACCAT TACTTCAGCG GCAGCTACAT GCATCAGGGC 
AGGCAGCCTA GCCCCGGCCT GTGCACTCTT TCGCAAAATC CGCTCGTGTC TGGCATTCCG 
GAGAGTCCCC CTGAAGACCA TCACCTCGCA ATGAACTGCT ACAGGATGAC CGACAGGTAC 
CATGCCTACC CAAACCCTCT TCAGCACGTC GGTCAGGAAG GGCACGCTCT GGAAACTCAC 
GGGATAGACC ACCTCCAGAA CTTGGCGTCC GTCTCAAACC ATCACCTGCA GAACCTGGAG 
GTCAACCGGC CTAGCGGGGG TCACGGCGTG TACCCGCCCT TCATGGGGAG GCCTGCGCCG 
ATCCAGCACT ACGACCATCA ACCCATGAAC AACCCCACGT TCAACCACAT CTCCCAGGCG 
CCTTCACCGC AGGGGGTCAT GCTTCCACCC CAGTCTTACA TTGCCCAGCC CGTCTCGCCG 
CACCTGACGA GGAACGTCGG CTACGGCGTG CCCGGGCTCT CCAGGACCGG CGGCTTCCTC 
CCGTCTCCTC ACGAGGCCTT CTTATCTCAC CCCAGTCCTC CCGTCACCCC CATGACTCCT 
CCCAGCACAC CGAGGCTCAC GGACCTCAAT CCCGTCAGGA TCGACTCTCC GCTCCACTTG 
CGAGCCTCTT ACTACTCGGC CGCGACGAGT CCCTTCGGCG TGCCGGTCGA CCCGTCCTAC 
TCTCCGCTGG CCAGGAGCCA CAGTCAGGGG CCCGCCTTTG CGCCATACAT GGACGGAGCG 
GCCTGGGCCT CCGACCGTGA CTACAACAGT GCGGCTCTCC AAAACTCCTT TTACGTGCCG 
CGCTCCCAGT GCTTCCCGAC GGCAGCACCG CCGCTGCCCC AGAAAGACTA TGCGTTCCGC 
GCGCCCCCGG GCAGCCACTT TGCAGGACCT TCTCTTCCCT CTTCGTCGCG TCTTCCGCCC 
GGCCTGGCCA CGCCTCCGCA GCAGGACCGC TCTGCCACCG CCAGCGTGCT TCCCCCGTTC 
AAGAACACGT TTCTCCCGGC GCAGTCTGCC CTCAACGGTT TCGAGCGTCA CCTGCAGACG 
TTCAAGTCGG TGATGATGGA AGACGCCGCG CAGAGGAGCT CCGCCTCTGG GCTCTACGGC 
GCTCCGGCCC CCACGGGCCG CCCTTCTCAG CGGCCAGCTA GTCACGAGGG CTTCACGAGG 
ACGCCTCTCG AACCGCCCGT CACGGAAGAG TCTTCCGAGG GGATCTACGA GGTGGACTCG 
GACAACGAAA GCGCCTTCCA GGACGAAGAG GTTGGCGGCG TCGCCATTGC GCTCACTCAC 
GGGTCGGTGC TCTTCGAGTG CGCCAAGCAC GAGCTGCATG CGACGACTGC TCTGCGGAGG 
CCCAACCGCA AGAACCCCAC CCGCATCAGC CTCGTGTTCT ACCAGCACAA GAACCTCAAC 
TTCCGGAATC ACGGGGAGGA GGAGTGGGAG AAGAAGATGG AGGTTCGCAA GTTGGAAAGA 
GCCAACGGGG AGCGGCCCTC CAAGAAGAAG GCCAAGCTCT CCGTGGACAG GTTGGATGAG 
GGTGGCGGCT TCCCCGCGGC TCCCGCAGAC GTCGGCCAGC CGTGGGAGGT GCCCCCCCGC 
GTGTCCCTGC CGTCGTTCTT CGCCATCAAC CCTTACCAGA AGTGTTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8042599 ( XM_002415629.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002415674.1 Ixodes scapularis hypothetical protein, mRNA.

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CloneID	OIa10828
Clone ID Related Accession (Same CDS sequence)	XM_002415629.1
Accession Version	XM_002415629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-22
Organism	Ixodes scapularis(black-legged tick)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002852970). COMPLETENESS: incomplete on both ends.

ATGGCGGCCT CTCCGGGCAA CCGAACTAAT CTCAACGGGC GGGCGGTGAA CGGCGGCGGC 
GCTTCGACGT CGCCCTACCT CCCGCAGGGA GTGCCGGAAC ACCTCGGACA TCCTGGGATC 
GGTGGCTTTC CCGGTCACAG CGAGTACCAG CTCCCGCCCA CGCAAAAGCC TGCCCCGCCG 
GCCCAGGCCG GCCAGATGTA CGAGATGGGG CCGCACTTCG GGGCCTTCCC GGGCCACCCG 
TCTCAGGCCC TGGCTTTCAA TGGCCACATG GCACAGCACG ACGCTGGCCG CCTGGGGCTC 
CCCGGCGAAG AGGGCGACTT CCCCACGCGC CACCAGATGA TGAACAGCCG TCTCAAGACA 
CTGATTCAGA CTAGGCAGAG CCAGAAGGAG CAGGGTCCCT TCCAGGGGCT CGAGACGACC 
CTAGGCCCCT CCGTCGGAGG AAGCTTTGTG CACCCGCAGG CCGATGCGAC GGCGACTGCC 
TCCCGGGGGT TCCCGCCGCT TGTGCGGAGC AACGGTGGCT GCGGTGCCGT GAGCGGCAAC 
AACAACGGCG GAAACAATGG CGGCGGCAAC AACAACAACA ACGGCACCAA CAACGGCGGT 
GGTAACAACG GCAACGGCGG CACGGGCGGC GGTAACAACA ATCCGAGGCC TCTGTCGTCA 
AACGAGATGC CCGGGGATGC GTGGCACGAT GCGAGCCCGT CCTCGGGTCC TCCGCCGGGC 
AGGGCTGGTG TCGCAGAGCT ACCGGAGTTT GCCAAGAACG GGTCCTCTTC CTCCTCCCAG 
GGAAGCAACA ACAACGCCAA CAACAACAAC TCCGAAGCTG AGTTCTCCCA GCCTGAGGAT 
CAGAGTCACG AGAGTGAGTT TGCAGAGGGT GGCGCTGCGG AAACCAGGGG AGGTGGCGGC 
GGCGACAGGT CGAGCGCTCC CCCCAACGGA ATGTTGCCCT ACGGAAGCTT CTATGGGGCG 
CAAGAGGCGG CAGAAGTCCG GAAGGGTGGC GAGGGCAAGG TCCCCCCGCC GACAACGAGC 
GCGGCGACGA CCACTTCGGC GGCGGACGTT CACGGTGGTG GTGCGCATTA CCAGTTCACG 
TCGGCAGCGA CTTCGATGAG CACGACGACG ACGAGTGCCT CCGTTCACGG CAAACCCTGC 
AACGGACCAA CGCAGCCTTC GTTTGCGGAG CGGCAGTCGC CCAGGGGCGG CCCGTTCTCC 
GGTGCACCGA CGTCATTCGC TCCGGCGCCC CACTTGGCGA GTATGCACCA AGAGCGCCCG 
GTCCCCGAAA GTCCGACGCA GCTCCGCAAC GAGGTAGGCG AAGCCGGCAG CGGCGGCCAG 
ACGCGCGGCA ATGAGAGCGA ATCCACGGAC GCGGCGGCAG TCACCTCGCA GCCTTTTTTA 
TCGAATACGA CCACATCTAT GAATACATAT ACAAGTATTA TCAATACATA TAACAGCTTC 
GCCGCTTCGT GCGCGCCGTC CTTCTTGGCC GAGCACCAGC AACCCAAGTT TGGCGGGAGC 
GTGGAGGATG CGGCTCGAAC GGGGGGAGGA GACGCATCCG ACGTAGATGG TGGTCGTATT 
GCCGTCGGCG AATTCCGTGG ACTCGACTTT GGGGCACCCC CCCTGCCTCA CCACCCCGGT 
GGCCCCGAGG GCCCCAGGCC GCACTACCCC CAGTACGGGG GGGCGCCTGC CGCGTTTGTG 
CCAGAGGGTC CTTACGCTGT TCCCTATGGG GCCGGGAACC TGGGGAATCT GGGGAACCCG 
TTCCCCTTGC CCCCCATGGG CGCCGGAGGT CCTCCCTTTG CGCCCATGAG CCTCCCTGGT 
GCCGACCCCT GGTGGGAGCG GCTGGAGCGG CTACGCAGCA ACGCCAAGGC GGAGCCTCCG 
GCCTGCGACT GCTATGGCGC GGATGAGACG CGTGAGTACC GGACCCCCCG CAGTCCGTCC 
CTGGCCTCCA GTCTGCCTGC CGCCATGCTG AACGAACGGG GCCCTGCGTT GCGCATCGAG 
AAGGTGCTCT ACTCGGGCAA GGAGGGCAAG ACGAGCCAGG GCTGCCCGGT GGCCAAGTGG 
ATCATCCGGC GCAGCGGCCC TTCGGAGAAG GTGCTGGCCG TGCTGCGGCA CCGACCGGGC 
CACCGCTGCT TGTCGGCCTA CATCGTGATG GCCATCGTCG CCTGGGAGGG CGTCCAGGCA 
GACATGGCAG ACGACCTCTA CAGGACTGTG ACCCACAAGA CCGTCAACTT TGGCTTCCCC 
ACTCAGCGGA GATGCGGCAC CAACGAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002415674.1
CDS	1..2253
Translation	MAASPGNRTNLNGRAVNGGGASTSPYLPQGVPEHLGHPGIGGFPGHSEYQLPPTQKPAPPAQAGQMYEMGPHFGAFPGHPSQALAFNGHMAQHDAGRLGLPGEEGDFPTRHQMMNSRLKTLIQTRQSQKEQGPFQGLETTLGPSVGGSFVHPQADATATASRGFPPLVRSNGGCGAVSGNNNGGNNGGGNNNNNGTNNGGGNNGNGGTGGGNNNPRPLSSNEMPGDAWHDASPSSGPPPGRAGVAELPEFAKNGSSSSSQGSNNNANNNNSEAEFSQPEDQSHESEFAEGGAAETRGGGGGDRSSAPPNGMLPYGSFYGAQEAAEVRKGGEGKVPPPTTSAATTTSAADVHGGGAHYQFTSAATSMSTTTTSASVHGKPCNGPTQPSFAERQSPRGGPFSGAPTSFAPAPHLASMHQERPVPESPTQLRNEVGEAGSGGQTRGNESESTDAAAVTSQPFLSNTTTSMNTYTSIINTYNSFAASCAPSFLAEHQQPKFGGSVEDAARTGGGDASDVDGGRIAVGEFRGLDFGAPPLPHHPGGPEGPRPHYPQYGGAPAAFVPEGPYAVPYGAGNLGNLGNPFPLPPMGAGGPPFAPMSLPGADPWWERLERLRSNAKAEPPACDCYGADETREYRTPRSPSLASSLPAAMLNERGPALRIEKVLYSGKEGKTSQGCPVAKWIIRRSGPSEKVLAVLRHRPGHRCLSAYIVMAIVAWEGVQADMADDLYRTVTHKTVNFGFPTQRRCGTNEQ

Target ORF information:

RefSeq Version	XM_002415629.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002415629.1

ORF Insert Sequence:

ATGGCGGCCT CTCCGGGCAA CCGAACTAAT CTCAACGGGC GGGCGGTGAA CGGCGGCGGC 
GCTTCGACGT CGCCCTACCT CCCGCAGGGA GTGCCGGAAC ACCTCGGACA TCCTGGGATC 
GGTGGCTTTC CCGGTCACAG CGAGTACCAG CTCCCGCCCA CGCAAAAGCC TGCCCCGCCG 
GCCCAGGCCG GCCAGATGTA CGAGATGGGG CCGCACTTCG GGGCCTTCCC GGGCCACCCG 
TCTCAGGCCC TGGCTTTCAA TGGCCACATG GCACAGCACG ACGCTGGCCG CCTGGGGCTC 
CCCGGCGAAG AGGGCGACTT CCCCACGCGC CACCAGATGA TGAACAGCCG TCTCAAGACA 
CTGATTCAGA CTAGGCAGAG CCAGAAGGAG CAGGGTCCCT TCCAGGGGCT CGAGACGACC 
CTAGGCCCCT CCGTCGGAGG AAGCTTTGTG CACCCGCAGG CCGATGCGAC GGCGACTGCC 
TCCCGGGGGT TCCCGCCGCT TGTGCGGAGC AACGGTGGCT GCGGTGCCGT GAGCGGCAAC 
AACAACGGCG GAAACAATGG CGGCGGCAAC AACAACAACA ACGGCACCAA CAACGGCGGT 
GGTAACAACG GCAACGGCGG CACGGGCGGC GGTAACAACA ATCCGAGGCC TCTGTCGTCA 
AACGAGATGC CCGGGGATGC GTGGCACGAT GCGAGCCCGT CCTCGGGTCC TCCGCCGGGC 
AGGGCTGGTG TCGCAGAGCT ACCGGAGTTT GCCAAGAACG GGTCCTCTTC CTCCTCCCAG 
GGAAGCAACA ACAACGCCAA CAACAACAAC TCCGAAGCTG AGTTCTCCCA GCCTGAGGAT 
CAGAGTCACG AGAGTGAGTT TGCAGAGGGT GGCGCTGCGG AAACCAGGGG AGGTGGCGGC 
GGCGACAGGT CGAGCGCTCC CCCCAACGGA ATGTTGCCCT ACGGAAGCTT CTATGGGGCG 
CAAGAGGCGG CAGAAGTCCG GAAGGGTGGC GAGGGCAAGG TCCCCCCGCC GACAACGAGC 
GCGGCGACGA CCACTTCGGC GGCGGACGTT CACGGTGGTG GTGCGCATTA CCAGTTCACG 
TCGGCAGCGA CTTCGATGAG CACGACGACG ACGAGTGCCT CCGTTCACGG CAAACCCTGC 
AACGGACCAA CGCAGCCTTC GTTTGCGGAG CGGCAGTCGC CCAGGGGCGG CCCGTTCTCC 
GGTGCACCGA CGTCATTCGC TCCGGCGCCC CACTTGGCGA GTATGCACCA AGAGCGCCCG 
GTCCCCGAAA GTCCGACGCA GCTCCGCAAC GAGGTAGGCG AAGCCGGCAG CGGCGGCCAG 
ACGCGCGGCA ATGAGAGCGA ATCCACGGAC GCGGCGGCAG TCACCTCGCA GCCTTTTTTA 
TCGAATACGA CCACATCTAT GAATACATAT ACAAGTATTA TCAATACATA TAACAGCTTC 
GCCGCTTCGT GCGCGCCGTC CTTCTTGGCC GAGCACCAGC AACCCAAGTT TGGCGGGAGC 
GTGGAGGATG CGGCTCGAAC GGGGGGAGGA GACGCATCCG ACGTAGATGG TGGTCGTATT 
GCCGTCGGCG AATTCCGTGG ACTCGACTTT GGGGCACCCC CCCTGCCTCA CCACCCCGGT 
GGCCCCGAGG GCCCCAGGCC GCACTACCCC CAGTACGGGG GGGCGCCTGC CGCGTTTGTG 
CCAGAGGGTC CTTACGCTGT TCCCTATGGG GCCGGGAACC TGGGGAATCT GGGGAACCCG 
TTCCCCTTGC CCCCCATGGG CGCCGGAGGT CCTCCCTTTG CGCCCATGAG CCTCCCTGGT 
GCCGACCCCT GGTGGGAGCG GCTGGAGCGG CTACGCAGCA ACGCCAAGGC GGAGCCTCCG 
GCCTGCGACT GCTATGGCGC GGATGAGACG CGTGAGTACC GGACCCCCCG CAGTCCGTCC 
CTGGCCTCCA GTCTGCCTGC CGCCATGCTG AACGAACGGG GCCCTGCGTT GCGCATCGAG 
AAGGTGCTCT ACTCGGGCAA GGAGGGCAAG ACGAGCCAGG GCTGCCCGGT GGCCAAGTGG 
ATCATCCGGC GCAGCGGCCC TTCGGAGAAG GTGCTGGCCG TGCTGCGGCA CCGACCGGGC 
CACCGCTGCT TGTCGGCCTA CATCGTGATG GCCATCGTCG CCTGGGAGGG CGTCCAGGCA 
GACATGGCAG ACGACCTCTA CAGGACTGTG ACCCACAAGA CCGTCAACTT TGGCTTCCCC 
ACTCAGCGGA GATGCGGCAC CAACGAACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8042599 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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