IscW_ISCW006533 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	IscW_ISCW006533
Entrez Gene ID	8050645
Full Name	Niemann-Pick type C1 domain-containing protein, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029968672.1	XP_029824532.1	NPC intracellular cholesterol transporter 1 isoform X2
XM_029968666.1	XP_029824526.1	NPC intracellular cholesterol transporter 1 isoform X1
XM_029968677.1	XP_029824537.1	NPC intracellular cholesterol transporter 1 isoform X3
XM_002435602.1	XP_002435647.1	Niemann-Pick type C1 domain-containing protein, putative

Pathways from BioSystems
Gene Ontology

The following IscW_ISCW006533 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IscW_ISCW006533 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa16882	XM_029968666.1 Latest version!	Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OIa16883	XM_029968672.1 Latest version!	Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OIa16884	XM_029968677.1 Latest version!	Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OIa12400	XM_002435602.1 Latest version!	Ixodes scapularis Niemann-Pick type C1 domain-containing protein, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IscW_ISCW006533 ( XM_029968666.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029824526.1 Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa16882
Clone ID Related Accession (Same CDS sequence)	XM_029968666.1
Accession Version	XM_029968666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	NPC intracellular cholesterol transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021918062.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACATT CCCGGACCCA TTCTAAATTA TCCCGTGCTG TTGTGAACTC TAAAATCTCG 
TACGGCATCG CAGCCGTCAC AATGGATACC GGCAGGCGAC GCGTCACGAC TGTGCTCAGG 
ATTTTAGTGT GTCTACTCGT CGTGCTGGCA CAGGGCACTC CTGCAGAAAG TAGATGTTCC 
TTTCGAGGAA AATGCGGCAT AGACGAAGAC ACAGAGAAGG AACGACCGTG CCTATATACA 
GGGGAGGGAC AGCCGATGCA AGAAGAAGGC TTGAGCATCC TTCGGGAGCT GTGTCCTCAT 
TTGATAGAAG GTCAGGCAGA GCCAAAATTC TGCTGTGACT ACCCGCAGCT GGTAACGATG 
AAGGCCGAGC TGGAACAGCC CCTCGGCCTG GGCATGGGCA AGTGTCCCAC GTGCTACAGC 
AACTTTGTCC GCATCTTCTG CGGCTTTTGC GACCCAAACC ACGCCGACTA CATCGCCATC 
AACCGGACCG AGCCGAGTGA CGAGCAGGAA GGGAAGGAGA CGGTCCTGGC CGTGGACTAC 
GCCGTCAGCG AAGAGTTTGC GCACGGGGCC TTCGACTCGT GTGCCCACGT GCAGTCGGTG 
GTGACGGACA ACACGGTGAT GCAGTTCATG TGCGGTAGCA AGGGCAAGGA CTGCACGGCG 
GCCGACTGGT TCACCTTCCT GGGAAGCACC TCCGACGAGA ACGGCTTTGC TCCGCTCAAG 
TTCAACTACA TCATCACGGG GGAGAGGAGC ATTACGGCCA GCGGTGTTTC CCTCAAGCCG 
TTCAACCCAG GCCACCATCG ATGCTCGGAG CCATTTGGAG CCAGCAAGCA GCGGTGCAGT 
TGCAGCGACT GCCCGGAGGT TTGCGTGGCC TTGGAGCCAC CGCTGCTGCC ACCAGACGCG 
ATGCCTTTCA CCATTGGTCG CTACGACGGA ATGCTGGTGG TTTCCATGCT CCTCTTTGTG 
CTCCTCAGCG CCGGCGTCCT CGGAATTTTC TTCCTCAAGA GCAACAGGAG GCGGAGCTCT 
TTCAGAGGGG TACCCAAATG TGGTGCCTTT TCATCAGTTA CATCAGTGTC AGGCTCATCG 
GAGTCCAGTG TTTCCGACAC GAGCGGCAAC AACAATGACA TTATCAGGAT GCCGCTGCAC 
CGGCCAACCA AACCGGCCCG TTCCGTTTTG TTGGCGGCTG TGGATCTTGG CTCCCTTGAG 
GAGGTGGAGC CCCTCAGGTC AAAGCGCTCA ATCAGCTCCA ACGGCTTCAC CAACGCCTCA 
GCCGTGGACT CCCCGACGCC ATCTCCTCCT GGATCTCCTA TCTCGCTCCG GCCGCCGCCG 
CTGTCTGGAG ATGCTGTCAA CAACGGCAGC GTTGCCAACG GCAGCGCGGT GAAGCCCCCC 
GCGGAGGAGC CACACGTCCG CACGCTCTCG TCGTTCGGGG CCACCATGGA ACAACTGCTG 
CAGGCCGGCT TCCACCGCTG GGGCCTCTTT GTTGCCCGCA ACCCCCTCCT GGTGCTCATC 
GCCGCGCTGG CTGTGAGCGT CCTCCTGTCT CTGGGTCTGC TCAAGTTTAC TGTGACTACG 
AACCCTGTGG ACCTGTGGGT GTCACATAGC AGTCTGGCCA GGAAACACAT GAACTACTTC 
AACAGTCACT TTGGTCCTTT CTACCGGGTG GAGCAGATAA TCCTCCGTCC CAAGAACCAG 
CAGTTCTTTG TGCTGGAGAC GGATGGAGTA AACAGAACAT TTGGGCCGGC ATTCGAGAAG 
AATTTTATGA TGGAGGCCCT GAGGCTGCAG CTGGCAGTGG AAGCAGTGGT GGGCAGGCTG 
CGTCTCGATG GGACGCCGAT CCACGAGTCG GAAGAAGAGG TTTTGGATCC GAAGGACGTG 
AAGAACGTCA CCATTCAGGA CGTCTGCCTG TCCCCACTGT CCCCGCTGAA CAGGCACTGT 
TCGGTGCAGA GCATCTTCGC CTACTACCAA GACGACCCGT CCAAGCTCAA CCTGACGGAC 
AAGTTGGATC CGCTCTCTTA CCTCAAGCAC TTCGAACACT GCAGCAAAAC TCCGTCTGAC 
GTCAACTGCT TCGCCAAGTA TGGAGGACCG ATCGATGACA TCTCCCTGGT TCTGGGGGGA 
TTCAACGGGA GTGACTTTCA CCTGGCCTCT GCTCTGGTGA TCACCATACC GGTGAACAAC 
TTCAACGACG TAGAGAAGAA GTACCCTGCC CTCGCCTGGG AGAAAGAGTT CGTCAAGCTC 
ATGAAGAACT ACAACAACAC CGAGGTCATG ACTGTTGCCT TCATGGCTGA GAGATCTATC 
GAAGACGAAC TGGAGCGAGG CAGTCACTCT GACGTCGTGA CGGTTGGCAT CAGCTACGTC 
ATCATGTTCG CCTACATCGC CATTGCGCTG GGCGACATCA ACAGTTGCAG TAGACTTTTG 
ATTGACTCAA AGATTTCCCT GGGCCTGGTT GGAGTGATAA TCGTTCTTCT GTCGGTGGTG 
GCCTCGCTGG GCATCTTCAG CTTCTTTGGC GTTTCCGCCA CGCTCATCAT TGTTGAAGTG 
ATCCCCTTCC TGGTGCTTGC TGTTGGGGTC GACAACATTT TCATCCTGGT GCAGCAGTTT 
CAGCGCGATG TCCGTCGCGA GGGAGAGACA ACGGTGGAGC AGGTGGGGAG GCTGGTAGGA 
GAGGTTGCCC CAAGCATGAT GCTCTCCAGT GTCTCCATGT CTGCCTGCTT CTTCATCGGT 
GCCTTGACAG AAACTCCCGC AGTGCGGATC TTTGCGCTGT ACGCCGGTGT TGCCCTGCTC 
ATCAACTTTT TCCTGCAGAT GACTTGCTTC CTCTCCTTGT TTACCCTGGA CACACTTAGG 
CAGGAGGACG GCCGTCTAGA TCTGTGCTTC TGCATCCGTG CTTCAAAGAA GAGCCGTCCG 
TCCCAAAACA CCAGTCTGCT ATACAAGTTC TTCAAGAAAG TCTACGCACC CTTCTTGCTG 
AACAACTCCG TGCGTGTGGT GGTGATGATC ACGTTCATTG GCTGGCTCTG TTCGTCCCTC 
GCCGTCATCG GAAAAATTGA AGTTGGGCTG GATCAGGAGC TGGCCATGCC TCAGGATTCC 
TACCTTCAGC AGTACTTTGA CTACCTCAAG AAGTACCTCC AAGTAGGACC GCCGGTGTAC 
TTCATGGTCA CGGAGGGCTA TGACTACTCC AAGGTTGAGA ACCAGGCAAA ACTCTGCATC 
CAGGAGCAAG TGTGTGATCA GGACTCTGTG GGTGCTAAGT TGAAGCAGCT CACACTGCTC 
AGCAACAGGA CTTACGTGAC GAGGTTACGG TCTTACTGGT TGGACCAGTA CATCCTGTAC 
ATGCGGTCGT CCGGCTGTTG CTACGAGGGC CAGAACAGCT CAGACTTCTG CTATTCGCAG 
TATGGTGATG GGGGTGGAAA ATGCAAAAGT TGTCACGTTC CGAGGGAGAA GCCCTTCGTA 
GGAGAGGAGT TTTCGCATTA CCTCTCTTGG TTCCTGAAGG ACGTACCGGG CGCCAAGTGT 
TCTTCAGCAG GTCGTGCGGA ACATGGTGGT TCGATTGATC ACGTAAACGG AACTATTAAA 
TCTGCCTACT TTTCGGCCTA CCACCCTGTC CTGAAAACAT CTAAGGACTT CTACACGGCA 
CTCGACTGGG CTCGGCTCAT CTCCCACAAC CTGACCCAAG ACATACAGGC CATTCAGCCA 
GGAGTGCAAG TCATCCCTTA TAGCCTGGTG CACGTGTTCT ACGAGCAGTA CCTCACCATG 
TGGCCGGACA CGTTCAAGAA CCTGGCCCTG TCGCTGGGTG CCATATTTGT GGTCACCTTT 
GTGCTGCTGG GGCTGGACTT TGTGTCTGCG ACGGTGGTCA CCTTCACCAT CGTCATGATC 
ATCGTCAACC TGATGGGCCT GATGTACTGG TGGGACATCT CGCTCAACGC AGTGTCCCTC 
GTCAACCTCG TGGTGGGCGT GGGGATCTCG GTGGAGTTCT GCTCCCACCT GGTGCGAGTG 
TTTGCTCTCA GCGGTGCCCC GTCCCGAGTC AAGAGGGCCC AGGACGCACT CACCAAGATG 
GGCAGTTCGA TCCTGTCCGG AATCACGCTG ACGGATTGTG GCATCCTGGT GCTAGCCTTT 
GCCAAGTCGC AGATATTCCA GGTTTTCTAT TTCCGCATGT ACCTGGGCAT CATTGCCTTT 
GGCACGCTGC ACAGCCTCAT CTTCCTCCCG GTGTTTCTAA GCATCCTGGG TCCCCCCGTC 
AACAAGGACA AGTTGATAGA GCACATTCAG CTCCAGGAGA TGGCCACAGT CACTACGGCC 
GTCGTGAGGT CCAAGGCAGA CACCTACACT CGAAAGGACG TCAGCGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029824526.1
CDS	242..4552
Translation	MSHSRTHSKLSRAVVNSKISYGIAAVTMDTGRRRVTTVLRILVCLLVVLAQGTPAESRCSFRGKCGIDEDTEKERPCLYTGEGQPMQEEGLSILRELCPHLIEGQAEPKFCCDYPQLVTMKAELEQPLGLGMGKCPTCYSNFVRIFCGFCDPNHADYIAINRTEPSDEQEGKETVLAVDYAVSEEFAHGAFDSCAHVQSVVTDNTVMQFMCGSKGKDCTAADWFTFLGSTSDENGFAPLKFNYIITGERSITASGVSLKPFNPGHHRCSEPFGASKQRCSCSDCPEVCVALEPPLLPPDAMPFTIGRYDGMLVVSMLLFVLLSAGVLGIFFLKSNRRRSSFRGVPKCGAFSSVTSVSGSSESSVSDTSGNNNDIIRMPLHRPTKPARSVLLAAVDLGSLEEVEPLRSKRSISSNGFTNASAVDSPTPSPPGSPISLRPPPLSGDAVNNGSVANGSAVKPPAEEPHVRTLSSFGATMEQLLQAGFHRWGLFVARNPLLVLIAALAVSVLLSLGLLKFTVTTNPVDLWVSHSSLARKHMNYFNSHFGPFYRVEQIILRPKNQQFFVLETDGVNRTFGPAFEKNFMMEALRLQLAVEAVVGRLRLDGTPIHESEEEVLDPKDVKNVTIQDVCLSPLSPLNRHCSVQSIFAYYQDDPSKLNLTDKLDPLSYLKHFEHCSKTPSDVNCFAKYGGPIDDISLVLGGFNGSDFHLASALVITIPVNNFNDVEKKYPALAWEKEFVKLMKNYNNTEVMTVAFMAERSIEDELERGSHSDVVTVGISYVIMFAYIAIALGDINSCSRLLIDSKISLGLVGVIIVLLSVVASLGIFSFFGVSATLIIVEVIPFLVLAVGVDNIFILVQQFQRDVRREGETTVEQVGRLVGEVAPSMMLSSVSMSACFFIGALTETPAVRIFALYAGVALLINFFLQMTCFLSLFTLDTLRQEDGRLDLCFCIRASKKSRPSQNTSLLYKFFKKVYAPFLLNNSVRVVVMITFIGWLCSSLAVIGKIEVGLDQELAMPQDSYLQQYFDYLKKYLQVGPPVYFMVTEGYDYSKVENQAKLCIQEQVCDQDSVGAKLKQLTLLSNRTYVTRLRSYWLDQYILYMRSSGCCYEGQNSSDFCYSQYGDGGGKCKSCHVPREKPFVGEEFSHYLSWFLKDVPGAKCSSAGRAEHGGSIDHVNGTIKSAYFSAYHPVLKTSKDFYTALDWARLISHNLTQDIQAIQPGVQVIPYSLVHVFYEQYLTMWPDTFKNLALSLGAIFVVTFVLLGLDFVSATVVTFTIVMIIVNLMGLMYWWDISLNAVSLVNLVVGVGISVEFCSHLVRVFALSGAPSRVKRAQDALTKMGSSILSGITLTDCGILVLAFAKSQIFQVFYFRMYLGIIAFGTLHSLIFLPVFLSILGPPVNKDKLIEHIQLQEMATVTTAVVRSKADTYTRKDVSA

Target ORF information:

RefSeq Version	XM_029968666.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029968666.1

ORF Insert Sequence:

ATGTCACATT CCCGGACCCA TTCTAAATTA TCCCGTGCTG TTGTGAACTC TAAAATCTCG 
TACGGCATCG CAGCCGTCAC AATGGATACC GGCAGGCGAC GCGTCACGAC TGTGCTCAGG 
ATTTTAGTGT GTCTACTCGT CGTGCTGGCA CAGGGCACTC CTGCAGAAAG TAGATGTTCC 
TTTCGAGGAA AATGCGGCAT AGACGAAGAC ACAGAGAAGG AACGACCGTG CCTATATACA 
GGGGAGGGAC AGCCGATGCA AGAAGAAGGC TTGAGCATCC TTCGGGAGCT GTGTCCTCAT 
TTGATAGAAG GTCAGGCAGA GCCAAAATTC TGCTGTGACT ACCCGCAGCT GGTAACGATG 
AAGGCCGAGC TGGAACAGCC CCTCGGCCTG GGCATGGGCA AGTGTCCCAC GTGCTACAGC 
AACTTTGTCC GCATCTTCTG CGGCTTTTGC GACCCAAACC ACGCCGACTA CATCGCCATC 
AACCGGACCG AGCCGAGTGA CGAGCAGGAA GGGAAGGAGA CGGTCCTGGC CGTGGACTAC 
GCCGTCAGCG AAGAGTTTGC GCACGGGGCC TTCGACTCGT GTGCCCACGT GCAGTCGGTG 
GTGACGGACA ACACGGTGAT GCAGTTCATG TGCGGTAGCA AGGGCAAGGA CTGCACGGCG 
GCCGACTGGT TCACCTTCCT GGGAAGCACC TCCGACGAGA ACGGCTTTGC TCCGCTCAAG 
TTCAACTACA TCATCACGGG GGAGAGGAGC ATTACGGCCA GCGGTGTTTC CCTCAAGCCG 
TTCAACCCAG GCCACCATCG ATGCTCGGAG CCATTTGGAG CCAGCAAGCA GCGGTGCAGT 
TGCAGCGACT GCCCGGAGGT TTGCGTGGCC TTGGAGCCAC CGCTGCTGCC ACCAGACGCG 
ATGCCTTTCA CCATTGGTCG CTACGACGGA ATGCTGGTGG TTTCCATGCT CCTCTTTGTG 
CTCCTCAGCG CCGGCGTCCT CGGAATTTTC TTCCTCAAGA GCAACAGGAG GCGGAGCTCT 
TTCAGAGGGG TACCCAAATG TGGTGCCTTT TCATCAGTTA CATCAGTGTC AGGCTCATCG 
GAGTCCAGTG TTTCCGACAC GAGCGGCAAC AACAATGACA TTATCAGGAT GCCGCTGCAC 
CGGCCAACCA AACCGGCCCG TTCCGTTTTG TTGGCGGCTG TGGATCTTGG CTCCCTTGAG 
GAGGTGGAGC CCCTCAGGTC AAAGCGCTCA ATCAGCTCCA ACGGCTTCAC CAACGCCTCA 
GCCGTGGACT CCCCGACGCC ATCTCCTCCT GGATCTCCTA TCTCGCTCCG GCCGCCGCCG 
CTGTCTGGAG ATGCTGTCAA CAACGGCAGC GTTGCCAACG GCAGCGCGGT GAAGCCCCCC 
GCGGAGGAGC CACACGTCCG CACGCTCTCG TCGTTCGGGG CCACCATGGA ACAACTGCTG 
CAGGCCGGCT TCCACCGCTG GGGCCTCTTT GTTGCCCGCA ACCCCCTCCT GGTGCTCATC 
GCCGCGCTGG CTGTGAGCGT CCTCCTGTCT CTGGGTCTGC TCAAGTTTAC TGTGACTACG 
AACCCTGTGG ACCTGTGGGT GTCACATAGC AGTCTGGCCA GGAAACACAT GAACTACTTC 
AACAGTCACT TTGGTCCTTT CTACCGGGTG GAGCAGATAA TCCTCCGTCC CAAGAACCAG 
CAGTTCTTTG TGCTGGAGAC GGATGGAGTA AACAGAACAT TTGGGCCGGC ATTCGAGAAG 
AATTTTATGA TGGAGGCCCT GAGGCTGCAG CTGGCAGTGG AAGCAGTGGT GGGCAGGCTG 
CGTCTCGATG GGACGCCGAT CCACGAGTCG GAAGAAGAGG TTTTGGATCC GAAGGACGTG 
AAGAACGTCA CCATTCAGGA CGTCTGCCTG TCCCCACTGT CCCCGCTGAA CAGGCACTGT 
TCGGTGCAGA GCATCTTCGC CTACTACCAA GACGACCCGT CCAAGCTCAA CCTGACGGAC 
AAGTTGGATC CGCTCTCTTA CCTCAAGCAC TTCGAACACT GCAGCAAAAC TCCGTCTGAC 
GTCAACTGCT TCGCCAAGTA TGGAGGACCG ATCGATGACA TCTCCCTGGT TCTGGGGGGA 
TTCAACGGGA GTGACTTTCA CCTGGCCTCT GCTCTGGTGA TCACCATACC GGTGAACAAC 
TTCAACGACG TAGAGAAGAA GTACCCTGCC CTCGCCTGGG AGAAAGAGTT CGTCAAGCTC 
ATGAAGAACT ACAACAACAC CGAGGTCATG ACTGTTGCCT TCATGGCTGA GAGATCTATC 
GAAGACGAAC TGGAGCGAGG CAGTCACTCT GACGTCGTGA CGGTTGGCAT CAGCTACGTC 
ATCATGTTCG CCTACATCGC CATTGCGCTG GGCGACATCA ACAGTTGCAG TAGACTTTTG 
ATTGACTCAA AGATTTCCCT GGGCCTGGTT GGAGTGATAA TCGTTCTTCT GTCGGTGGTG 
GCCTCGCTGG GCATCTTCAG CTTCTTTGGC GTTTCCGCCA CGCTCATCAT TGTTGAAGTG 
ATCCCCTTCC TGGTGCTTGC TGTTGGGGTC GACAACATTT TCATCCTGGT GCAGCAGTTT 
CAGCGCGATG TCCGTCGCGA GGGAGAGACA ACGGTGGAGC AGGTGGGGAG GCTGGTAGGA 
GAGGTTGCCC CAAGCATGAT GCTCTCCAGT GTCTCCATGT CTGCCTGCTT CTTCATCGGT 
GCCTTGACAG AAACTCCCGC AGTGCGGATC TTTGCGCTGT ACGCCGGTGT TGCCCTGCTC 
ATCAACTTTT TCCTGCAGAT GACTTGCTTC CTCTCCTTGT TTACCCTGGA CACACTTAGG 
CAGGAGGACG GCCGTCTAGA TCTGTGCTTC TGCATCCGTG CTTCAAAGAA GAGCCGTCCG 
TCCCAAAACA CCAGTCTGCT ATACAAGTTC TTCAAGAAAG TCTACGCACC CTTCTTGCTG 
AACAACTCCG TGCGTGTGGT GGTGATGATC ACGTTCATTG GCTGGCTCTG TTCGTCCCTC 
GCCGTCATCG GAAAAATTGA AGTTGGGCTG GATCAGGAGC TGGCCATGCC TCAGGATTCC 
TACCTTCAGC AGTACTTTGA CTACCTCAAG AAGTACCTCC AAGTAGGACC GCCGGTGTAC 
TTCATGGTCA CGGAGGGCTA TGACTACTCC AAGGTTGAGA ACCAGGCAAA ACTCTGCATC 
CAGGAGCAAG TGTGTGATCA GGACTCTGTG GGTGCTAAGT TGAAGCAGCT CACACTGCTC 
AGCAACAGGA CTTACGTGAC GAGGTTACGG TCTTACTGGT TGGACCAGTA CATCCTGTAC 
ATGCGGTCGT CCGGCTGTTG CTACGAGGGC CAGAACAGCT CAGACTTCTG CTATTCGCAG 
TATGGTGATG GGGGTGGAAA ATGCAAAAGT TGTCACGTTC CGAGGGAGAA GCCCTTCGTA 
GGAGAGGAGT TTTCGCATTA CCTCTCTTGG TTCCTGAAGG ACGTACCGGG CGCCAAGTGT 
TCTTCAGCAG GTCGTGCGGA ACATGGTGGT TCGATTGATC ACGTAAACGG AACTATTAAA 
TCTGCCTACT TTTCGGCCTA CCACCCTGTC CTGAAAACAT CTAAGGACTT CTACACGGCA 
CTCGACTGGG CTCGGCTCAT CTCCCACAAC CTGACCCAAG ACATACAGGC CATTCAGCCA 
GGAGTGCAAG TCATCCCTTA TAGCCTGGTG CACGTGTTCT ACGAGCAGTA CCTCACCATG 
TGGCCGGACA CGTTCAAGAA CCTGGCCCTG TCGCTGGGTG CCATATTTGT GGTCACCTTT 
GTGCTGCTGG GGCTGGACTT TGTGTCTGCG ACGGTGGTCA CCTTCACCAT CGTCATGATC 
ATCGTCAACC TGATGGGCCT GATGTACTGG TGGGACATCT CGCTCAACGC AGTGTCCCTC 
GTCAACCTCG TGGTGGGCGT GGGGATCTCG GTGGAGTTCT GCTCCCACCT GGTGCGAGTG 
TTTGCTCTCA GCGGTGCCCC GTCCCGAGTC AAGAGGGCCC AGGACGCACT CACCAAGATG 
GGCAGTTCGA TCCTGTCCGG AATCACGCTG ACGGATTGTG GCATCCTGGT GCTAGCCTTT 
GCCAAGTCGC AGATATTCCA GGTTTTCTAT TTCCGCATGT ACCTGGGCAT CATTGCCTTT 
GGCACGCTGC ACAGCCTCAT CTTCCTCCCG GTGTTTCTAA GCATCCTGGG TCCCCCCGTC 
AACAAGGACA AGTTGATAGA GCACATTCAG CTCCAGGAGA TGGCCACAGT CACTACGGCC 
GTCGTGAGGT CCAAGGCAGA CACCTACACT CGAAAGGACG TCAGCGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW006533 ( XM_029968672.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029824532.1 Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIa16883
Clone ID Related Accession (Same CDS sequence)	XM_029968672.1
Accession Version	XM_029968672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	NPC intracellular cholesterol transporter 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021918062.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACATT CCCGGACCCA TTCTAAATTA TCCCGTGCTG TTGTGAACTC TAAAATCTCG 
TACGGCATCG CAGCCGTCAC AATGGATACC GGCAGGCGAC GCGTCACGAC TGTGCTCAGG 
ATTTTAGTGT GTCTACTCGT CGTGCTGGCA CAGGGCACTC CTGCAGAAAG TAGATGTTCC 
TTTCGAGGAA AATGCGGCAT AGACGAAGAC ACAGAGAAGG AACGACCGTG CCTATATACA 
GGGGAGGGAC AGCCGATGCA AGAAGAAGGC TTGAGCATCC TTCGGGAGCT GTGTCCTCAT 
TTGATAGAAG GTCAGGCAGA GCCAAAATTC TGCTGTGACT ACCCGCAGCT GGTAACGATG 
AAGGCCGAGC TGGAACAGCC CCTCGGCCTG GGCATGGGCA AGTGTCCCAC GTGCTACAGC 
AACTTTGTCC GCATCTTCTG CGGCTTTTGC GACCCAAACC ACGCCGACTA CATCGCCATC 
AACCGGACCG AGCCGAGTGA CGAGCAGGAA GGGAAGGAGA CGGTCCTGGC CGTGGACTAC 
GCCGTCAGCG AAGAGTTTGC GCACGGGGCC TTCGACTCGT GTGCCCACGT GCAGTCGGTG 
GTGACGGACA ACACGGTGAT GCAGTTCATG TGCGGTAGCA AGGGCAAGGA CTGCACGGCG 
GCCGACTGGT TCACCTTCCT GGGAAGCACC TCCGACGAGA ACGGCTTTGC TCCGCTCAAG 
TTCAACTACA TCATCACGGG GGAGAGGAGC ATTACGGCCA GCGGTGTTTC CCTCAAGCCG 
TTCAACCCAG GCCACCATCG ATGCTCGGAG CCATTTGGAG CCAGCAAGCA GCGGTGCAGT 
TGCAGCGACT GCCCGGAGGT TTGCGTGGCC TTGGAGCCAC CGCTGCTGCC ACCAGACGCG 
ATGCCTTTCA CCATTGGTCG CTACGACGGA ATGCTGGTGG TTTCCATGCT CCTCTTTGTG 
CTCCTCAGCG CCGGCGTCCT CGGAATTTTC TTCCTCAAGA GCAACAGGAG GCGGAGCTCT 
TTCAGAGTGT CAGGCTCATC GGAGTCCAGT GTTTCCGACA CGAGCGGCAA CAACAATGAC 
ATTATCAGGA TGCCGCTGCA CCGGCCAACC AAACCGGCCC GTTCCGTTTT GTTGGCGGCT 
GTGGATCTTG GCTCCCTTGA GGAGGTGGAG CCCCTCAGGT CAAAGCGCTC AATCAGCTCC 
AACGGCTTCA CCAACGCCTC AGCCGTGGAC TCCCCGACGC CATCTCCTCC TGGATCTCCT 
ATCTCGCTCC GGCCGCCGCC GCTGTCTGGA GATGCTGTCA ACAACGGCAG CGTTGCCAAC 
GGCAGCGCGG TGAAGCCCCC CGCGGAGGAG CCACACGTCC GCACGCTCTC GTCGTTCGGG 
GCCACCATGG AACAACTGCT GCAGGCCGGC TTCCACCGCT GGGGCCTCTT TGTTGCCCGC 
AACCCCCTCC TGGTGCTCAT CGCCGCGCTG GCTGTGAGCG TCCTCCTGTC TCTGGGTCTG 
CTCAAGTTTA CTGTGACTAC GAACCCTGTG GACCTGTGGG TGTCACATAG CAGTCTGGCC 
AGGAAACACA TGAACTACTT CAACAGTCAC TTTGGTCCTT TCTACCGGGT GGAGCAGATA 
ATCCTCCGTC CCAAGAACCA GCAGTTCTTT GTGCTGGAGA CGGATGGAGT AAACAGAACA 
TTTGGGCCGG CATTCGAGAA GAATTTTATG ATGGAGGCCC TGAGGCTGCA GCTGGCAGTG 
GAAGCAGTGG TGGGCAGGCT GCGTCTCGAT GGGACGCCGA TCCACGAGTC GGAAGAAGAG 
GTTTTGGATC CGAAGGACGT GAAGAACGTC ACCATTCAGG ACGTCTGCCT GTCCCCACTG 
TCCCCGCTGA ACAGGCACTG TTCGGTGCAG AGCATCTTCG CCTACTACCA AGACGACCCG 
TCCAAGCTCA ACCTGACGGA CAAGTTGGAT CCGCTCTCTT ACCTCAAGCA CTTCGAACAC 
TGCAGCAAAA CTCCGTCTGA CGTCAACTGC TTCGCCAAGT ATGGAGGACC GATCGATGAC 
ATCTCCCTGG TTCTGGGGGG ATTCAACGGG AGTGACTTTC ACCTGGCCTC TGCTCTGGTG 
ATCACCATAC CGGTGAACAA CTTCAACGAC GTAGAGAAGA AGTACCCTGC CCTCGCCTGG 
GAGAAAGAGT TCGTCAAGCT CATGAAGAAC TACAACAACA CCGAGGTCAT GACTGTTGCC 
TTCATGGCTG AGAGATCTAT CGAAGACGAA CTGGAGCGAG GCAGTCACTC TGACGTCGTG 
ACGGTTGGCA TCAGCTACGT CATCATGTTC GCCTACATCG CCATTGCGCT GGGCGACATC 
AACAGTTGCA GTAGACTTTT GATTGACTCA AAGATTTCCC TGGGCCTGGT TGGAGTGATA 
ATCGTTCTTC TGTCGGTGGT GGCCTCGCTG GGCATCTTCA GCTTCTTTGG CGTTTCCGCC 
ACGCTCATCA TTGTTGAAGT GATCCCCTTC CTGGTGCTTG CTGTTGGGGT CGACAACATT 
TTCATCCTGG TGCAGCAGTT TCAGCGCGAT GTCCGTCGCG AGGGAGAGAC AACGGTGGAG 
CAGGTGGGGA GGCTGGTAGG AGAGGTTGCC CCAAGCATGA TGCTCTCCAG TGTCTCCATG 
TCTGCCTGCT TCTTCATCGG TGCCTTGACA GAAACTCCCG CAGTGCGGAT CTTTGCGCTG 
TACGCCGGTG TTGCCCTGCT CATCAACTTT TTCCTGCAGA TGACTTGCTT CCTCTCCTTG 
TTTACCCTGG ACACACTTAG GCAGGAGGAC GGCCGTCTAG ATCTGTGCTT CTGCATCCGT 
GCTTCAAAGA AGAGCCGTCC GTCCCAAAAC ACCAGTCTGC TATACAAGTT CTTCAAGAAA 
GTCTACGCAC CCTTCTTGCT GAACAACTCC GTGCGTGTGG TGGTGATGAT CACGTTCATT 
GGCTGGCTCT GTTCGTCCCT CGCCGTCATC GGAAAAATTG AAGTTGGGCT GGATCAGGAG 
CTGGCCATGC CTCAGGATTC CTACCTTCAG CAGTACTTTG ACTACCTCAA GAAGTACCTC 
CAAGTAGGAC CGCCGGTGTA CTTCATGGTC ACGGAGGGCT ATGACTACTC CAAGGTTGAG 
AACCAGGCAA AACTCTGCAT CCAGGAGCAA GTGTGTGATC AGGACTCTGT GGGTGCTAAG 
TTGAAGCAGC TCACACTGCT CAGCAACAGG ACTTACGTGA CGAGGTTACG GTCTTACTGG 
TTGGACCAGT ACATCCTGTA CATGCGGTCG TCCGGCTGTT GCTACGAGGG CCAGAACAGC 
TCAGACTTCT GCTATTCGCA GTATGGTGAT GGGGGTGGAA AATGCAAAAG TTGTCACGTT 
CCGAGGGAGA AGCCCTTCGT AGGAGAGGAG TTTTCGCATT ACCTCTCTTG GTTCCTGAAG 
GACGTACCGG GCGCCAAGTG TTCTTCAGCA GGTCGTGCGG AACATGGTGG TTCGATTGAT 
CACGTAAACG GAACTATTAA ATCTGCCTAC TTTTCGGCCT ACCACCCTGT CCTGAAAACA 
TCTAAGGACT TCTACACGGC ACTCGACTGG GCTCGGCTCA TCTCCCACAA CCTGACCCAA 
GACATACAGG CCATTCAGCC AGGAGTGCAA GTCATCCCTT ATAGCCTGGT GCACGTGTTC 
TACGAGCAGT ACCTCACCAT GTGGCCGGAC ACGTTCAAGA ACCTGGCCCT GTCGCTGGGT 
GCCATATTTG TGGTCACCTT TGTGCTGCTG GGGCTGGACT TTGTGTCTGC GACGGTGGTC 
ACCTTCACCA TCGTCATGAT CATCGTCAAC CTGATGGGCC TGATGTACTG GTGGGACATC 
TCGCTCAACG CAGTGTCCCT CGTCAACCTC GTGGTGGGCG TGGGGATCTC GGTGGAGTTC 
TGCTCCCACC TGGTGCGAGT GTTTGCTCTC AGCGGTGCCC CGTCCCGAGT CAAGAGGGCC 
CAGGACGCAC TCACCAAGAT GGGCAGTTCG ATCCTGTCCG GAATCACGCT GACGGATTGT 
GGCATCCTGG TGCTAGCCTT TGCCAAGTCG CAGATATTCC AGGTTTTCTA TTTCCGCATG 
TACCTGGGCA TCATTGCCTT TGGCACGCTG CACAGCCTCA TCTTCCTCCC GGTGTTTCTA 
AGCATCCTGG GTCCCCCCGT CAACAAGGAC AAGTTGATAG AGCACATTCA GCTCCAGGAG 
ATGGCCACAG TCACTACGGC CGTCGTGAGG TCCAAGGCAG ACACCTACAC TCGAAAGGAC 
GTCAGCGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029824532.1
CDS	242..4513
Translation	MSHSRTHSKLSRAVVNSKISYGIAAVTMDTGRRRVTTVLRILVCLLVVLAQGTPAESRCSFRGKCGIDEDTEKERPCLYTGEGQPMQEEGLSILRELCPHLIEGQAEPKFCCDYPQLVTMKAELEQPLGLGMGKCPTCYSNFVRIFCGFCDPNHADYIAINRTEPSDEQEGKETVLAVDYAVSEEFAHGAFDSCAHVQSVVTDNTVMQFMCGSKGKDCTAADWFTFLGSTSDENGFAPLKFNYIITGERSITASGVSLKPFNPGHHRCSEPFGASKQRCSCSDCPEVCVALEPPLLPPDAMPFTIGRYDGMLVVSMLLFVLLSAGVLGIFFLKSNRRRSSFRVSGSSESSVSDTSGNNNDIIRMPLHRPTKPARSVLLAAVDLGSLEEVEPLRSKRSISSNGFTNASAVDSPTPSPPGSPISLRPPPLSGDAVNNGSVANGSAVKPPAEEPHVRTLSSFGATMEQLLQAGFHRWGLFVARNPLLVLIAALAVSVLLSLGLLKFTVTTNPVDLWVSHSSLARKHMNYFNSHFGPFYRVEQIILRPKNQQFFVLETDGVNRTFGPAFEKNFMMEALRLQLAVEAVVGRLRLDGTPIHESEEEVLDPKDVKNVTIQDVCLSPLSPLNRHCSVQSIFAYYQDDPSKLNLTDKLDPLSYLKHFEHCSKTPSDVNCFAKYGGPIDDISLVLGGFNGSDFHLASALVITIPVNNFNDVEKKYPALAWEKEFVKLMKNYNNTEVMTVAFMAERSIEDELERGSHSDVVTVGISYVIMFAYIAIALGDINSCSRLLIDSKISLGLVGVIIVLLSVVASLGIFSFFGVSATLIIVEVIPFLVLAVGVDNIFILVQQFQRDVRREGETTVEQVGRLVGEVAPSMMLSSVSMSACFFIGALTETPAVRIFALYAGVALLINFFLQMTCFLSLFTLDTLRQEDGRLDLCFCIRASKKSRPSQNTSLLYKFFKKVYAPFLLNNSVRVVVMITFIGWLCSSLAVIGKIEVGLDQELAMPQDSYLQQYFDYLKKYLQVGPPVYFMVTEGYDYSKVENQAKLCIQEQVCDQDSVGAKLKQLTLLSNRTYVTRLRSYWLDQYILYMRSSGCCYEGQNSSDFCYSQYGDGGGKCKSCHVPREKPFVGEEFSHYLSWFLKDVPGAKCSSAGRAEHGGSIDHVNGTIKSAYFSAYHPVLKTSKDFYTALDWARLISHNLTQDIQAIQPGVQVIPYSLVHVFYEQYLTMWPDTFKNLALSLGAIFVVTFVLLGLDFVSATVVTFTIVMIIVNLMGLMYWWDISLNAVSLVNLVVGVGISVEFCSHLVRVFALSGAPSRVKRAQDALTKMGSSILSGITLTDCGILVLAFAKSQIFQVFYFRMYLGIIAFGTLHSLIFLPVFLSILGPPVNKDKLIEHIQLQEMATVTTAVVRSKADTYTRKDVSA

Target ORF information:

RefSeq Version	XM_029968672.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029968672.1

ORF Insert Sequence:

ATGTCACATT CCCGGACCCA TTCTAAATTA TCCCGTGCTG TTGTGAACTC TAAAATCTCG 
TACGGCATCG CAGCCGTCAC AATGGATACC GGCAGGCGAC GCGTCACGAC TGTGCTCAGG 
ATTTTAGTGT GTCTACTCGT CGTGCTGGCA CAGGGCACTC CTGCAGAAAG TAGATGTTCC 
TTTCGAGGAA AATGCGGCAT AGACGAAGAC ACAGAGAAGG AACGACCGTG CCTATATACA 
GGGGAGGGAC AGCCGATGCA AGAAGAAGGC TTGAGCATCC TTCGGGAGCT GTGTCCTCAT 
TTGATAGAAG GTCAGGCAGA GCCAAAATTC TGCTGTGACT ACCCGCAGCT GGTAACGATG 
AAGGCCGAGC TGGAACAGCC CCTCGGCCTG GGCATGGGCA AGTGTCCCAC GTGCTACAGC 
AACTTTGTCC GCATCTTCTG CGGCTTTTGC GACCCAAACC ACGCCGACTA CATCGCCATC 
AACCGGACCG AGCCGAGTGA CGAGCAGGAA GGGAAGGAGA CGGTCCTGGC CGTGGACTAC 
GCCGTCAGCG AAGAGTTTGC GCACGGGGCC TTCGACTCGT GTGCCCACGT GCAGTCGGTG 
GTGACGGACA ACACGGTGAT GCAGTTCATG TGCGGTAGCA AGGGCAAGGA CTGCACGGCG 
GCCGACTGGT TCACCTTCCT GGGAAGCACC TCCGACGAGA ACGGCTTTGC TCCGCTCAAG 
TTCAACTACA TCATCACGGG GGAGAGGAGC ATTACGGCCA GCGGTGTTTC CCTCAAGCCG 
TTCAACCCAG GCCACCATCG ATGCTCGGAG CCATTTGGAG CCAGCAAGCA GCGGTGCAGT 
TGCAGCGACT GCCCGGAGGT TTGCGTGGCC TTGGAGCCAC CGCTGCTGCC ACCAGACGCG 
ATGCCTTTCA CCATTGGTCG CTACGACGGA ATGCTGGTGG TTTCCATGCT CCTCTTTGTG 
CTCCTCAGCG CCGGCGTCCT CGGAATTTTC TTCCTCAAGA GCAACAGGAG GCGGAGCTCT 
TTCAGAGTGT CAGGCTCATC GGAGTCCAGT GTTTCCGACA CGAGCGGCAA CAACAATGAC 
ATTATCAGGA TGCCGCTGCA CCGGCCAACC AAACCGGCCC GTTCCGTTTT GTTGGCGGCT 
GTGGATCTTG GCTCCCTTGA GGAGGTGGAG CCCCTCAGGT CAAAGCGCTC AATCAGCTCC 
AACGGCTTCA CCAACGCCTC AGCCGTGGAC TCCCCGACGC CATCTCCTCC TGGATCTCCT 
ATCTCGCTCC GGCCGCCGCC GCTGTCTGGA GATGCTGTCA ACAACGGCAG CGTTGCCAAC 
GGCAGCGCGG TGAAGCCCCC CGCGGAGGAG CCACACGTCC GCACGCTCTC GTCGTTCGGG 
GCCACCATGG AACAACTGCT GCAGGCCGGC TTCCACCGCT GGGGCCTCTT TGTTGCCCGC 
AACCCCCTCC TGGTGCTCAT CGCCGCGCTG GCTGTGAGCG TCCTCCTGTC TCTGGGTCTG 
CTCAAGTTTA CTGTGACTAC GAACCCTGTG GACCTGTGGG TGTCACATAG CAGTCTGGCC 
AGGAAACACA TGAACTACTT CAACAGTCAC TTTGGTCCTT TCTACCGGGT GGAGCAGATA 
ATCCTCCGTC CCAAGAACCA GCAGTTCTTT GTGCTGGAGA CGGATGGAGT AAACAGAACA 
TTTGGGCCGG CATTCGAGAA GAATTTTATG ATGGAGGCCC TGAGGCTGCA GCTGGCAGTG 
GAAGCAGTGG TGGGCAGGCT GCGTCTCGAT GGGACGCCGA TCCACGAGTC GGAAGAAGAG 
GTTTTGGATC CGAAGGACGT GAAGAACGTC ACCATTCAGG ACGTCTGCCT GTCCCCACTG 
TCCCCGCTGA ACAGGCACTG TTCGGTGCAG AGCATCTTCG CCTACTACCA AGACGACCCG 
TCCAAGCTCA ACCTGACGGA CAAGTTGGAT CCGCTCTCTT ACCTCAAGCA CTTCGAACAC 
TGCAGCAAAA CTCCGTCTGA CGTCAACTGC TTCGCCAAGT ATGGAGGACC GATCGATGAC 
ATCTCCCTGG TTCTGGGGGG ATTCAACGGG AGTGACTTTC ACCTGGCCTC TGCTCTGGTG 
ATCACCATAC CGGTGAACAA CTTCAACGAC GTAGAGAAGA AGTACCCTGC CCTCGCCTGG 
GAGAAAGAGT TCGTCAAGCT CATGAAGAAC TACAACAACA CCGAGGTCAT GACTGTTGCC 
TTCATGGCTG AGAGATCTAT CGAAGACGAA CTGGAGCGAG GCAGTCACTC TGACGTCGTG 
ACGGTTGGCA TCAGCTACGT CATCATGTTC GCCTACATCG CCATTGCGCT GGGCGACATC 
AACAGTTGCA GTAGACTTTT GATTGACTCA AAGATTTCCC TGGGCCTGGT TGGAGTGATA 
ATCGTTCTTC TGTCGGTGGT GGCCTCGCTG GGCATCTTCA GCTTCTTTGG CGTTTCCGCC 
ACGCTCATCA TTGTTGAAGT GATCCCCTTC CTGGTGCTTG CTGTTGGGGT CGACAACATT 
TTCATCCTGG TGCAGCAGTT TCAGCGCGAT GTCCGTCGCG AGGGAGAGAC AACGGTGGAG 
CAGGTGGGGA GGCTGGTAGG AGAGGTTGCC CCAAGCATGA TGCTCTCCAG TGTCTCCATG 
TCTGCCTGCT TCTTCATCGG TGCCTTGACA GAAACTCCCG CAGTGCGGAT CTTTGCGCTG 
TACGCCGGTG TTGCCCTGCT CATCAACTTT TTCCTGCAGA TGACTTGCTT CCTCTCCTTG 
TTTACCCTGG ACACACTTAG GCAGGAGGAC GGCCGTCTAG ATCTGTGCTT CTGCATCCGT 
GCTTCAAAGA AGAGCCGTCC GTCCCAAAAC ACCAGTCTGC TATACAAGTT CTTCAAGAAA 
GTCTACGCAC CCTTCTTGCT GAACAACTCC GTGCGTGTGG TGGTGATGAT CACGTTCATT 
GGCTGGCTCT GTTCGTCCCT CGCCGTCATC GGAAAAATTG AAGTTGGGCT GGATCAGGAG 
CTGGCCATGC CTCAGGATTC CTACCTTCAG CAGTACTTTG ACTACCTCAA GAAGTACCTC 
CAAGTAGGAC CGCCGGTGTA CTTCATGGTC ACGGAGGGCT ATGACTACTC CAAGGTTGAG 
AACCAGGCAA AACTCTGCAT CCAGGAGCAA GTGTGTGATC AGGACTCTGT GGGTGCTAAG 
TTGAAGCAGC TCACACTGCT CAGCAACAGG ACTTACGTGA CGAGGTTACG GTCTTACTGG 
TTGGACCAGT ACATCCTGTA CATGCGGTCG TCCGGCTGTT GCTACGAGGG CCAGAACAGC 
TCAGACTTCT GCTATTCGCA GTATGGTGAT GGGGGTGGAA AATGCAAAAG TTGTCACGTT 
CCGAGGGAGA AGCCCTTCGT AGGAGAGGAG TTTTCGCATT ACCTCTCTTG GTTCCTGAAG 
GACGTACCGG GCGCCAAGTG TTCTTCAGCA GGTCGTGCGG AACATGGTGG TTCGATTGAT 
CACGTAAACG GAACTATTAA ATCTGCCTAC TTTTCGGCCT ACCACCCTGT CCTGAAAACA 
TCTAAGGACT TCTACACGGC ACTCGACTGG GCTCGGCTCA TCTCCCACAA CCTGACCCAA 
GACATACAGG CCATTCAGCC AGGAGTGCAA GTCATCCCTT ATAGCCTGGT GCACGTGTTC 
TACGAGCAGT ACCTCACCAT GTGGCCGGAC ACGTTCAAGA ACCTGGCCCT GTCGCTGGGT 
GCCATATTTG TGGTCACCTT TGTGCTGCTG GGGCTGGACT TTGTGTCTGC GACGGTGGTC 
ACCTTCACCA TCGTCATGAT CATCGTCAAC CTGATGGGCC TGATGTACTG GTGGGACATC 
TCGCTCAACG CAGTGTCCCT CGTCAACCTC GTGGTGGGCG TGGGGATCTC GGTGGAGTTC 
TGCTCCCACC TGGTGCGAGT GTTTGCTCTC AGCGGTGCCC CGTCCCGAGT CAAGAGGGCC 
CAGGACGCAC TCACCAAGAT GGGCAGTTCG ATCCTGTCCG GAATCACGCT GACGGATTGT 
GGCATCCTGG TGCTAGCCTT TGCCAAGTCG CAGATATTCC AGGTTTTCTA TTTCCGCATG 
TACCTGGGCA TCATTGCCTT TGGCACGCTG CACAGCCTCA TCTTCCTCCC GGTGTTTCTA 
AGCATCCTGG GTCCCCCCGT CAACAAGGAC AAGTTGATAG AGCACATTCA GCTCCAGGAG 
ATGGCCACAG TCACTACGGC CGTCGTGAGG TCCAAGGCAG ACACCTACAC TCGAAAGGAC 
GTCAGCGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW006533 ( XM_029968677.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029824537.1 Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X3, mRNA.

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CloneID	OIa16884
Clone ID Related Accession (Same CDS sequence)	XM_029968677.1
Accession Version	XM_029968677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	NPC intracellular cholesterol transporter 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021918062.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACATT CCCGGACCCA TTCTAAATTA TCCCGTGCTG TTGTGAACTC TAAAATCTCG 
TACGGCATCG CAGCCGTCAC AATGGATACC GGCAGGCGAC GCGTCACGAC TGTGCTCAGG 
ATTTTAGTGT GTCTACTCGT CGTGCTGGCA CAGGGCACTC CTGCAGAAAG TAGATGTTCC 
TTTCGAGGAA AATGCGGCAT AGACGAAGAC ACAGAGAAGG AACGACCGTG CCTATATACA 
GGGGAGGGAC AGCCGATGCA AGAAGAAGGC TTGAGCATCC TTCGGGAGCT GTGTCCTCAT 
TTGATAGAAG GTCAGGCAGA GCCAAAATTC TGCTGTGACT ACCCGCAGCT GGTAACGATG 
AAGGCCGAGC TGGAACAGCC CCTCGGCCTG GGCATGGGCA AGTGTCCCAC GTGCTACAGC 
AACTTTGTCC GCATCTTCTG CGGCTTTTGC GACCCAAACC ACGCCGACTA CATCGCCATC 
AACCGGACCG AGCCGAGTGA CGAGCAGGAA GGGAAGGAGA CGGTCCTGGC CGTGGACTAC 
GCCGTCAGCG AAGAGTTTGC GCACGGGGCC TTCGACTCGT GTGCCCACGT GCAGTCGGTG 
GTGACGGACA ACACGGTGAT GCAGTTCATG TGCGGTAGCA AGGGCAAGGA CTGCACGGCG 
GCCGACTGGT TCACCTTCCT GGGAAGCACC TCCGACGAGA ACGGCTTTGC TCCGCTCAAG 
TTCAACTACA TCATCACGGG GGAGAGGAGC ATTACGGCCA GCGGTGTTTC CCTCAAGCCG 
TTCAACCCAG GCCACCATCG ATGCTCGGAG CCATTTGGAG CCAGCAAGCA GCGGTGCAGT 
TGCAGCGACT GCCCGGAGGT TTGCGTGGCC TTGGAGCCAC CGCTGCTGCC ACCAGACGCG 
ATGCCTTTCA CCATTGGTCG CTACGACGGA ATGCTGGTGG TTTCCATGCT CCTCTTTGTG 
CTCCTCAGCG CCGGCGTCCT CGGAATTTTC TTCCTCAAGA GCAACAGGAG GCGGAGCTCT 
TTCAGAGTCA GCTCCAACGG CTTCACCAAC GCCTCAGCCG TGGACTCCCC GACGCCATCT 
CCTCCTGGAT CTCCTATCTC GCTCCGGCCG CCGCCGCTGT CTGGAGATGC TGTCAACAAC 
GGCAGCGTTG CCAACGGCAG CGCGGTGAAG CCCCCCGCGG AGGAGCCACA CGTCCGCACG 
CTCTCGTCGT TCGGGGCCAC CATGGAACAA CTGCTGCAGG CCGGCTTCCA CCGCTGGGGC 
CTCTTTGTTG CCCGCAACCC CCTCCTGGTG CTCATCGCCG CGCTGGCTGT GAGCGTCCTC 
CTGTCTCTGG GTCTGCTCAA GTTTACTGTG ACTACGAACC CTGTGGACCT GTGGGTGTCA 
CATAGCAGTC TGGCCAGGAA ACACATGAAC TACTTCAACA GTCACTTTGG TCCTTTCTAC 
CGGGTGGAGC AGATAATCCT CCGTCCCAAG AACCAGCAGT TCTTTGTGCT GGAGACGGAT 
GGAGTAAACA GAACATTTGG GCCGGCATTC GAGAAGAATT TTATGATGGA GGCCCTGAGG 
CTGCAGCTGG CAGTGGAAGC AGTGGTGGGC AGGCTGCGTC TCGATGGGAC GCCGATCCAC 
GAGTCGGAAG AAGAGGTTTT GGATCCGAAG GACGTGAAGA ACGTCACCAT TCAGGACGTC 
TGCCTGTCCC CACTGTCCCC GCTGAACAGG CACTGTTCGG TGCAGAGCAT CTTCGCCTAC 
TACCAAGACG ACCCGTCCAA GCTCAACCTG ACGGACAAGT TGGATCCGCT CTCTTACCTC 
AAGCACTTCG AACACTGCAG CAAAACTCCG TCTGACGTCA ACTGCTTCGC CAAGTATGGA 
GGACCGATCG ATGACATCTC CCTGGTTCTG GGGGGATTCA ACGGGAGTGA CTTTCACCTG 
GCCTCTGCTC TGGTGATCAC CATACCGGTG AACAACTTCA ACGACGTAGA GAAGAAGTAC 
CCTGCCCTCG CCTGGGAGAA AGAGTTCGTC AAGCTCATGA AGAACTACAA CAACACCGAG 
GTCATGACTG TTGCCTTCAT GGCTGAGAGA TCTATCGAAG ACGAACTGGA GCGAGGCAGT 
CACTCTGACG TCGTGACGGT TGGCATCAGC TACGTCATCA TGTTCGCCTA CATCGCCATT 
GCGCTGGGCG ACATCAACAG TTGCAGTAGA CTTTTGATTG ACTCAAAGAT TTCCCTGGGC 
CTGGTTGGAG TGATAATCGT TCTTCTGTCG GTGGTGGCCT CGCTGGGCAT CTTCAGCTTC 
TTTGGCGTTT CCGCCACGCT CATCATTGTT GAAGTGATCC CCTTCCTGGT GCTTGCTGTT 
GGGGTCGACA ACATTTTCAT CCTGGTGCAG CAGTTTCAGC GCGATGTCCG TCGCGAGGGA 
GAGACAACGG TGGAGCAGGT GGGGAGGCTG GTAGGAGAGG TTGCCCCAAG CATGATGCTC 
TCCAGTGTCT CCATGTCTGC CTGCTTCTTC ATCGGTGCCT TGACAGAAAC TCCCGCAGTG 
CGGATCTTTG CGCTGTACGC CGGTGTTGCC CTGCTCATCA ACTTTTTCCT GCAGATGACT 
TGCTTCCTCT CCTTGTTTAC CCTGGACACA CTTAGGCAGG AGGACGGCCG TCTAGATCTG 
TGCTTCTGCA TCCGTGCTTC AAAGAAGAGC CGTCCGTCCC AAAACACCAG TCTGCTATAC 
AAGTTCTTCA AGAAAGTCTA CGCACCCTTC TTGCTGAACA ACTCCGTGCG TGTGGTGGTG 
ATGATCACGT TCATTGGCTG GCTCTGTTCG TCCCTCGCCG TCATCGGAAA AATTGAAGTT 
GGGCTGGATC AGGAGCTGGC CATGCCTCAG GATTCCTACC TTCAGCAGTA CTTTGACTAC 
CTCAAGAAGT ACCTCCAAGT AGGACCGCCG GTGTACTTCA TGGTCACGGA GGGCTATGAC 
TACTCCAAGG TTGAGAACCA GGCAAAACTC TGCATCCAGG AGCAAGTGTG TGATCAGGAC 
TCTGTGGGTG CTAAGTTGAA GCAGCTCACA CTGCTCAGCA ACAGGACTTA CGTGACGAGG 
TTACGGTCTT ACTGGTTGGA CCAGTACATC CTGTACATGC GGTCGTCCGG CTGTTGCTAC 
GAGGGCCAGA ACAGCTCAGA CTTCTGCTAT TCGCAGTATG GTGATGGGGG TGGAAAATGC 
AAAAGTTGTC ACGTTCCGAG GGAGAAGCCC TTCGTAGGAG AGGAGTTTTC GCATTACCTC 
TCTTGGTTCC TGAAGGACGT ACCGGGCGCC AAGTGTTCTT CAGCAGGTCG TGCGGAACAT 
GGTGGTTCGA TTGATCACGT AAACGGAACT ATTAAATCTG CCTACTTTTC GGCCTACCAC 
CCTGTCCTGA AAACATCTAA GGACTTCTAC ACGGCACTCG ACTGGGCTCG GCTCATCTCC 
CACAACCTGA CCCAAGACAT ACAGGCCATT CAGCCAGGAG TGCAAGTCAT CCCTTATAGC 
CTGGTGCACG TGTTCTACGA GCAGTACCTC ACCATGTGGC CGGACACGTT CAAGAACCTG 
GCCCTGTCGC TGGGTGCCAT ATTTGTGGTC ACCTTTGTGC TGCTGGGGCT GGACTTTGTG 
TCTGCGACGG TGGTCACCTT CACCATCGTC ATGATCATCG TCAACCTGAT GGGCCTGATG 
TACTGGTGGG ACATCTCGCT CAACGCAGTG TCCCTCGTCA ACCTCGTGGT GGGCGTGGGG 
ATCTCGGTGG AGTTCTGCTC CCACCTGGTG CGAGTGTTTG CTCTCAGCGG TGCCCCGTCC 
CGAGTCAAGA GGGCCCAGGA CGCACTCACC AAGATGGGCA GTTCGATCCT GTCCGGAATC 
ACGCTGACGG ATTGTGGCAT CCTGGTGCTA GCCTTTGCCA AGTCGCAGAT ATTCCAGGTT 
TTCTATTTCC GCATGTACCT GGGCATCATT GCCTTTGGCA CGCTGCACAG CCTCATCTTC 
CTCCCGGTGT TTCTAAGCAT CCTGGGTCCC CCCGTCAACA AGGACAAGTT GATAGAGCAC 
ATTCAGCTCC AGGAGATGGC CACAGTCACT ACGGCCGTCG TGAGGTCCAA GGCAGACACC 
TACACTCGAA AGGACGTCAG CGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029824537.1
CDS	242..4348
Translation	MSHSRTHSKLSRAVVNSKISYGIAAVTMDTGRRRVTTVLRILVCLLVVLAQGTPAESRCSFRGKCGIDEDTEKERPCLYTGEGQPMQEEGLSILRELCPHLIEGQAEPKFCCDYPQLVTMKAELEQPLGLGMGKCPTCYSNFVRIFCGFCDPNHADYIAINRTEPSDEQEGKETVLAVDYAVSEEFAHGAFDSCAHVQSVVTDNTVMQFMCGSKGKDCTAADWFTFLGSTSDENGFAPLKFNYIITGERSITASGVSLKPFNPGHHRCSEPFGASKQRCSCSDCPEVCVALEPPLLPPDAMPFTIGRYDGMLVVSMLLFVLLSAGVLGIFFLKSNRRRSSFRVSSNGFTNASAVDSPTPSPPGSPISLRPPPLSGDAVNNGSVANGSAVKPPAEEPHVRTLSSFGATMEQLLQAGFHRWGLFVARNPLLVLIAALAVSVLLSLGLLKFTVTTNPVDLWVSHSSLARKHMNYFNSHFGPFYRVEQIILRPKNQQFFVLETDGVNRTFGPAFEKNFMMEALRLQLAVEAVVGRLRLDGTPIHESEEEVLDPKDVKNVTIQDVCLSPLSPLNRHCSVQSIFAYYQDDPSKLNLTDKLDPLSYLKHFEHCSKTPSDVNCFAKYGGPIDDISLVLGGFNGSDFHLASALVITIPVNNFNDVEKKYPALAWEKEFVKLMKNYNNTEVMTVAFMAERSIEDELERGSHSDVVTVGISYVIMFAYIAIALGDINSCSRLLIDSKISLGLVGVIIVLLSVVASLGIFSFFGVSATLIIVEVIPFLVLAVGVDNIFILVQQFQRDVRREGETTVEQVGRLVGEVAPSMMLSSVSMSACFFIGALTETPAVRIFALYAGVALLINFFLQMTCFLSLFTLDTLRQEDGRLDLCFCIRASKKSRPSQNTSLLYKFFKKVYAPFLLNNSVRVVVMITFIGWLCSSLAVIGKIEVGLDQELAMPQDSYLQQYFDYLKKYLQVGPPVYFMVTEGYDYSKVENQAKLCIQEQVCDQDSVGAKLKQLTLLSNRTYVTRLRSYWLDQYILYMRSSGCCYEGQNSSDFCYSQYGDGGGKCKSCHVPREKPFVGEEFSHYLSWFLKDVPGAKCSSAGRAEHGGSIDHVNGTIKSAYFSAYHPVLKTSKDFYTALDWARLISHNLTQDIQAIQPGVQVIPYSLVHVFYEQYLTMWPDTFKNLALSLGAIFVVTFVLLGLDFVSATVVTFTIVMIIVNLMGLMYWWDISLNAVSLVNLVVGVGISVEFCSHLVRVFALSGAPSRVKRAQDALTKMGSSILSGITLTDCGILVLAFAKSQIFQVFYFRMYLGIIAFGTLHSLIFLPVFLSILGPPVNKDKLIEHIQLQEMATVTTAVVRSKADTYTRKDVSA

Target ORF information:

RefSeq Version	XM_029968677.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis NPC intracellular cholesterol transporter 1 (LOC8050645), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029968677.1

ORF Insert Sequence:

ATGTCACATT CCCGGACCCA TTCTAAATTA TCCCGTGCTG TTGTGAACTC TAAAATCTCG 
TACGGCATCG CAGCCGTCAC AATGGATACC GGCAGGCGAC GCGTCACGAC TGTGCTCAGG 
ATTTTAGTGT GTCTACTCGT CGTGCTGGCA CAGGGCACTC CTGCAGAAAG TAGATGTTCC 
TTTCGAGGAA AATGCGGCAT AGACGAAGAC ACAGAGAAGG AACGACCGTG CCTATATACA 
GGGGAGGGAC AGCCGATGCA AGAAGAAGGC TTGAGCATCC TTCGGGAGCT GTGTCCTCAT 
TTGATAGAAG GTCAGGCAGA GCCAAAATTC TGCTGTGACT ACCCGCAGCT GGTAACGATG 
AAGGCCGAGC TGGAACAGCC CCTCGGCCTG GGCATGGGCA AGTGTCCCAC GTGCTACAGC 
AACTTTGTCC GCATCTTCTG CGGCTTTTGC GACCCAAACC ACGCCGACTA CATCGCCATC 
AACCGGACCG AGCCGAGTGA CGAGCAGGAA GGGAAGGAGA CGGTCCTGGC CGTGGACTAC 
GCCGTCAGCG AAGAGTTTGC GCACGGGGCC TTCGACTCGT GTGCCCACGT GCAGTCGGTG 
GTGACGGACA ACACGGTGAT GCAGTTCATG TGCGGTAGCA AGGGCAAGGA CTGCACGGCG 
GCCGACTGGT TCACCTTCCT GGGAAGCACC TCCGACGAGA ACGGCTTTGC TCCGCTCAAG 
TTCAACTACA TCATCACGGG GGAGAGGAGC ATTACGGCCA GCGGTGTTTC CCTCAAGCCG 
TTCAACCCAG GCCACCATCG ATGCTCGGAG CCATTTGGAG CCAGCAAGCA GCGGTGCAGT 
TGCAGCGACT GCCCGGAGGT TTGCGTGGCC TTGGAGCCAC CGCTGCTGCC ACCAGACGCG 
ATGCCTTTCA CCATTGGTCG CTACGACGGA ATGCTGGTGG TTTCCATGCT CCTCTTTGTG 
CTCCTCAGCG CCGGCGTCCT CGGAATTTTC TTCCTCAAGA GCAACAGGAG GCGGAGCTCT 
TTCAGAGTCA GCTCCAACGG CTTCACCAAC GCCTCAGCCG TGGACTCCCC GACGCCATCT 
CCTCCTGGAT CTCCTATCTC GCTCCGGCCG CCGCCGCTGT CTGGAGATGC TGTCAACAAC 
GGCAGCGTTG CCAACGGCAG CGCGGTGAAG CCCCCCGCGG AGGAGCCACA CGTCCGCACG 
CTCTCGTCGT TCGGGGCCAC CATGGAACAA CTGCTGCAGG CCGGCTTCCA CCGCTGGGGC 
CTCTTTGTTG CCCGCAACCC CCTCCTGGTG CTCATCGCCG CGCTGGCTGT GAGCGTCCTC 
CTGTCTCTGG GTCTGCTCAA GTTTACTGTG ACTACGAACC CTGTGGACCT GTGGGTGTCA 
CATAGCAGTC TGGCCAGGAA ACACATGAAC TACTTCAACA GTCACTTTGG TCCTTTCTAC 
CGGGTGGAGC AGATAATCCT CCGTCCCAAG AACCAGCAGT TCTTTGTGCT GGAGACGGAT 
GGAGTAAACA GAACATTTGG GCCGGCATTC GAGAAGAATT TTATGATGGA GGCCCTGAGG 
CTGCAGCTGG CAGTGGAAGC AGTGGTGGGC AGGCTGCGTC TCGATGGGAC GCCGATCCAC 
GAGTCGGAAG AAGAGGTTTT GGATCCGAAG GACGTGAAGA ACGTCACCAT TCAGGACGTC 
TGCCTGTCCC CACTGTCCCC GCTGAACAGG CACTGTTCGG TGCAGAGCAT CTTCGCCTAC 
TACCAAGACG ACCCGTCCAA GCTCAACCTG ACGGACAAGT TGGATCCGCT CTCTTACCTC 
AAGCACTTCG AACACTGCAG CAAAACTCCG TCTGACGTCA ACTGCTTCGC CAAGTATGGA 
GGACCGATCG ATGACATCTC CCTGGTTCTG GGGGGATTCA ACGGGAGTGA CTTTCACCTG 
GCCTCTGCTC TGGTGATCAC CATACCGGTG AACAACTTCA ACGACGTAGA GAAGAAGTAC 
CCTGCCCTCG CCTGGGAGAA AGAGTTCGTC AAGCTCATGA AGAACTACAA CAACACCGAG 
GTCATGACTG TTGCCTTCAT GGCTGAGAGA TCTATCGAAG ACGAACTGGA GCGAGGCAGT 
CACTCTGACG TCGTGACGGT TGGCATCAGC TACGTCATCA TGTTCGCCTA CATCGCCATT 
GCGCTGGGCG ACATCAACAG TTGCAGTAGA CTTTTGATTG ACTCAAAGAT TTCCCTGGGC 
CTGGTTGGAG TGATAATCGT TCTTCTGTCG GTGGTGGCCT CGCTGGGCAT CTTCAGCTTC 
TTTGGCGTTT CCGCCACGCT CATCATTGTT GAAGTGATCC CCTTCCTGGT GCTTGCTGTT 
GGGGTCGACA ACATTTTCAT CCTGGTGCAG CAGTTTCAGC GCGATGTCCG TCGCGAGGGA 
GAGACAACGG TGGAGCAGGT GGGGAGGCTG GTAGGAGAGG TTGCCCCAAG CATGATGCTC 
TCCAGTGTCT CCATGTCTGC CTGCTTCTTC ATCGGTGCCT TGACAGAAAC TCCCGCAGTG 
CGGATCTTTG CGCTGTACGC CGGTGTTGCC CTGCTCATCA ACTTTTTCCT GCAGATGACT 
TGCTTCCTCT CCTTGTTTAC CCTGGACACA CTTAGGCAGG AGGACGGCCG TCTAGATCTG 
TGCTTCTGCA TCCGTGCTTC AAAGAAGAGC CGTCCGTCCC AAAACACCAG TCTGCTATAC 
AAGTTCTTCA AGAAAGTCTA CGCACCCTTC TTGCTGAACA ACTCCGTGCG TGTGGTGGTG 
ATGATCACGT TCATTGGCTG GCTCTGTTCG TCCCTCGCCG TCATCGGAAA AATTGAAGTT 
GGGCTGGATC AGGAGCTGGC CATGCCTCAG GATTCCTACC TTCAGCAGTA CTTTGACTAC 
CTCAAGAAGT ACCTCCAAGT AGGACCGCCG GTGTACTTCA TGGTCACGGA GGGCTATGAC 
TACTCCAAGG TTGAGAACCA GGCAAAACTC TGCATCCAGG AGCAAGTGTG TGATCAGGAC 
TCTGTGGGTG CTAAGTTGAA GCAGCTCACA CTGCTCAGCA ACAGGACTTA CGTGACGAGG 
TTACGGTCTT ACTGGTTGGA CCAGTACATC CTGTACATGC GGTCGTCCGG CTGTTGCTAC 
GAGGGCCAGA ACAGCTCAGA CTTCTGCTAT TCGCAGTATG GTGATGGGGG TGGAAAATGC 
AAAAGTTGTC ACGTTCCGAG GGAGAAGCCC TTCGTAGGAG AGGAGTTTTC GCATTACCTC 
TCTTGGTTCC TGAAGGACGT ACCGGGCGCC AAGTGTTCTT CAGCAGGTCG TGCGGAACAT 
GGTGGTTCGA TTGATCACGT AAACGGAACT ATTAAATCTG CCTACTTTTC GGCCTACCAC 
CCTGTCCTGA AAACATCTAA GGACTTCTAC ACGGCACTCG ACTGGGCTCG GCTCATCTCC 
CACAACCTGA CCCAAGACAT ACAGGCCATT CAGCCAGGAG TGCAAGTCAT CCCTTATAGC 
CTGGTGCACG TGTTCTACGA GCAGTACCTC ACCATGTGGC CGGACACGTT CAAGAACCTG 
GCCCTGTCGC TGGGTGCCAT ATTTGTGGTC ACCTTTGTGC TGCTGGGGCT GGACTTTGTG 
TCTGCGACGG TGGTCACCTT CACCATCGTC ATGATCATCG TCAACCTGAT GGGCCTGATG 
TACTGGTGGG ACATCTCGCT CAACGCAGTG TCCCTCGTCA ACCTCGTGGT GGGCGTGGGG 
ATCTCGGTGG AGTTCTGCTC CCACCTGGTG CGAGTGTTTG CTCTCAGCGG TGCCCCGTCC 
CGAGTCAAGA GGGCCCAGGA CGCACTCACC AAGATGGGCA GTTCGATCCT GTCCGGAATC 
ACGCTGACGG ATTGTGGCAT CCTGGTGCTA GCCTTTGCCA AGTCGCAGAT ATTCCAGGTT 
TTCTATTTCC GCATGTACCT GGGCATCATT GCCTTTGGCA CGCTGCACAG CCTCATCTTC 
CTCCCGGTGT TTCTAAGCAT CCTGGGTCCC CCCGTCAACA AGGACAAGTT GATAGAGCAC 
ATTCAGCTCC AGGAGATGGC CACAGTCACT ACGGCCGTCG TGAGGTCCAA GGCAGACACC 
TACACTCGAA AGGACGTCAG CGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW006533 ( XM_002435602.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002435647.1 Ixodes scapularis Niemann-Pick type C1 domain-containing protein, putative, mRNA.

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CloneID	OIa12400
Clone ID Related Accession (Same CDS sequence)	XM_002435602.1
Accession Version	XM_002435602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-25
Organism	Ixodes scapularis(black-legged tick)
Product	Niemann-Pick type C1 domain-containing protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002651035). COMPLETENESS: incomplete on the 5' end.

ATGAAGGCCG AGCTGGAACA GCCCCTCGGC CTGGGCATGG GCAAGTGTCC CACGTGCTAC 
AGCAACTTTG TCCGCATCTT TTGCGGCTTT TGCGACCCAA ACCACGCCGA CTACATCGCC 
ATCAACAGGA CCGAGCCGAG TGACGAGCAG GAAGGGAAGG AGACGGTCCT GGCCGTGGAC 
TACGCCGTCA GCAAAGAGTT TGCGCACGGG GCCTTCGACT CGTGTGCCCA CGTGCAGTCG 
GTGGTGACGG ACAACACGGT GATGCAGTTC ATGTGCGGTA GCAAGGGCAA GGACTGCACG 
GCGGCCGACT GGTTCACCTT CCTGGGAAGC ACCTCCGACG AGAACGGCTT TGCTCCGCTC 
AAGTTCAACT ACATCATCAC GGGGGAGAGG AGCATTACGG CCAGCGGCGT TTCCCTCAAG 
CCGTTCAACC CAGGCCACCA TCGATGCTCG GAGCCGTTTG GAGCCAGCAA GCAGCGGTGC 
AGTTGCAGCG ACTGCCCGGA GGTTTGCGTG GCCTTGGAGC CACCGCTGTT GCCACCGGAC 
GCGATGCCTT TCACCATTGG TCGCTACGAC GGGATGCTGG TGGTTTCCAT GCTCCTCTTT 
GTGCTCCTCA GCGCCGGCGT CCTCGGAGTT TTCTTCCTCA AGAGCAACAG GAGGCGGAGC 
TCGTTCAGAG TCAGCTCCAA CGGCTTCACC AACGCCTCGG CCGTGGACTC CCCGACACCA 
TCTCCTCCCG GCTCTCCTAT CTCGCTCCGG CCGCCGCCGC TGTCCGGAGA CGCGGTCAAC 
AACGGCAGCG TCGCCAACGG CAACGCCAAC GGCAGCGCGG TGAAGCCCCC CGCGGAGGAG 
CCGCACGTCC GCACGCTCTC GTCGTTCGGG GCCACCATGG AACAACTGCT GCAGGCCGGC 
TTCCACCGCT GGGGCCTCTT TGTTGCCCGC AACCCCCTCC TGGTGCTCAT CGCCGCGCTG 
GCTGTGAGCG TCCTCCTGTC TCTGGGTCTG CTCAAGTTTA CTGTGACTAC GAACCCCGTG 
GACCTGTGGG TGTCACACAG CAGTCTGGCC AGGAAACACA TGAACTACTT CAACAGTCAC 
TTTGGTCCTT TCTACCGGGT GGAGCAGATA ATCCTCCGTC CCAAGAACCA GCAGTTCTTT 
GTGCTGGAGA CGGACGGAGT AAACAGAACA TTTGGGCCGG CATTCGAGAA GAATTTTATG 
ATGGAGGTGG GTTATAAGCT TTTGTACCAC CTACAGGTTT TGGATCCGAA GGACGTGAAG 
AACGTCACCA TTCAGGACGT CTGCCTGTCC CCACTGTCCC CGCTGAACAG GCATTGTTCG 
GTGCAGAGCA TCTTCGCCTA CTACCAAGAC GACCCGTCCA AGCTCAACCT GACGGACAAG 
TTGGATCCGC TCTCTTACCT CAAGCACTTC GAACACTGCA GCAAAACTCC GTCTGACGTC 
AACTGCTTCG CCAAGTATGG AGGACCGATC GATGACATCT CCCTGGTTCT GGGGGGATTC 
AATGGGAGTG ACTTTCACCT GGCCTCTGCT CTGGTAATCA CCATACCGGT GAACAACTTC 
AACGACGTAG AGAAGAAGTA CCCTGCCCTC GCCTGGGAGA AAGAGTTCGT CAAGCTCATG 
AAGAACTACA ACAACACCGA GGTCATGACT GTTGCCTTCA TGGCTGAGAG ATCTATCGAA 
GACGAACTGG AGCGAGGCAG TCACTCTGAC GTCGTGACGG TTGGCATCAG CTACGTCATC 
ATGTTCGCCT ACATCGCCAT TGCGCTGGGC GACATCAACA GTTGCAGTAG ACTTTTGATT 
GACTCAAAGA TTTCCCTGGG CCTGGTTGGA GTGATAATTG TTCTTCTGTC CGTGGTGGCC 
TCGCTGGGCA TCTTCAGCTT CTTTGGCGTT TCTGCCACGC TCATCATTGT TGAAGTGATC 
CCCTTCCTGG TGCTTGCTGT TGGGGTCGAC AACATTTTCA TCCTGGTGCA GCAGTTTCAG 
CGCGATGTCC GTCGCGAGGG AGAGACAACG GTGGAGCAGG TGGGGAGGCT GGTAGGAGAG 
GTTGCCCCAA GCATGATGCT CTCCAGTGTC TCCATGTCTG CCTGCTTCTT CATCGGTGCC 
TTGACAGAAA CTCCCGCAGT GCGGATCTTT GCGCTGTACG CCGGTGTCGC CCTGCTCATC 
AACTTCTTCC TACAAATGAC TTGCTTCCTC TCCTTGTTTA CTCTGGACAC ACTTAGGCAG 
GAGGACGGCC GTCTAGATCT GTGCTTCTGC ATCCGTGCTT CAAAGAAGAG CCGTCCGTCC 
CAAAACACCA GTCTGCTGTA CAAGTTCTTC AAGAAAGTCT ACGCACCCTT CTTGCTGAAC 
AACTCCGTGC GTGTGGTGGT GATGATCACG TTCATTGGCT GGCTCTGTTC GTCCCTCGCC 
GTCATCGGAA AAATTGAAGT TGGGCTGGAT CAGGAGCTGG CCATGCCTAA GGATTCCTAC 
CTTCAGCAGT ACTTTGACTA CCTCAAGAAG TACCTCCAAG TAGGACCGCC GGTGTACTTC 
ATGGTCACGG AGGGCTATGA CTACTCCAAG GTGGAGAACC AGGCAAAACT CTGCATCCAG 
GAGCAAGTGT GTGATCAGGA CTCTGTGGGT GCTAAGTTGA AGCAGCTCAC ACTGCTCAGC 
AACAGGACTT ACGTGACGAG GTTACGGTCT TACTGGTTGG ACCAGTACAT CCTTTACATG 
CGGTCGTCCG GCTGTTGCTA CGAGGGCCAG AACAGCTCAG ACTTCTGCTA TTCGCAGTAT 
GGGGGTGGAA AATGCAAAAG TTGTCACGTT CCGAGGGAGA AGCCCTTCGT AGGAGAGGAG 
TTTTTGCATT ACCTCTCTTG GTTCCTGAAG GACGTACCGG GCGCCAAGTG TTCTTCAGCA 
GGTCGCGCGG AACATGGCGG TTCGATTGAT CACGTAAACG GAACTATTAA ATCTGCCTAC 
TTTTCGGCCT ACCACCCTGT CCTGAAGACG TCTAAGGACT TCTACACGGC ACTCGACTGG 
GCTCGGCTCA TCTCCCACAA CCTGACCCAG GACATACAGG CCATTCAGCC AGGAGTGCAA 
GTCATCCCTT ATAGCCTGGT GCACGTGTTC TACGAGCAGT ACCTCACCAT GTGGCCGGAC 
ACGTTCAAGA ACCTGGCCCT GTCGCTGGGT GCCATATTTG TGGTCACCTT TGTGCTGCTG 
GGGCTGGACT TTGTGTCTGC GACGGTGGTC ACCTTCACCA TCGTCATGAT CATCGTCAAC 
CTGATGGGCC TGATGTACTG GTGGGACATC TCGCTCAACG CGGTGTCCCT CGTCAACCTC 
GTGGTGGGCG TGGGGATCTC GGTGGAGTTC TGCTCCCACC TGGTGCGAGT GTTTGCTCTC 
AGCGGTGCCC CGTCCCGAGT CAAAAGGGCC CAGGACGCAC TCACCAAGAT GGGCAGTTCG 
ATCCTGTCCG GAATCACGCT GACGGATTGT GGCATCCTGG TGCTAGCCTT TGCCAAGTCG 
CAGATATTCC AGGTTTTCTA TTTCCGCATG TACCTGGGCA TCATTGCCTT TGGCACGCTG 
CACAGCCTCA TCTTCCTCCC GGTGTTTCTA AGCATCCTGG GTCCCCCCGT CAACAAGGAC 
AAGTTGATAG AGCACATTCA GCTCCAGGAG ATGGCCACAG TCACTACGGC CGTCGTGAGG 
TCCAAGGCAG ACACCTACAC TCGAAAGGAC GTCAGCGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002435647.1
CDS	1..3702
Translation	MKAELEQPLGLGMGKCPTCYSNFVRIFCGFCDPNHADYIAINRTEPSDEQEGKETVLAVDYAVSKEFAHGAFDSCAHVQSVVTDNTVMQFMCGSKGKDCTAADWFTFLGSTSDENGFAPLKFNYIITGERSITASGVSLKPFNPGHHRCSEPFGASKQRCSCSDCPEVCVALEPPLLPPDAMPFTIGRYDGMLVVSMLLFVLLSAGVLGVFFLKSNRRRSSFRVSSNGFTNASAVDSPTPSPPGSPISLRPPPLSGDAVNNGSVANGNANGSAVKPPAEEPHVRTLSSFGATMEQLLQAGFHRWGLFVARNPLLVLIAALAVSVLLSLGLLKFTVTTNPVDLWVSHSSLARKHMNYFNSHFGPFYRVEQIILRPKNQQFFVLETDGVNRTFGPAFEKNFMMEVGYKLLYHLQVLDPKDVKNVTIQDVCLSPLSPLNRHCSVQSIFAYYQDDPSKLNLTDKLDPLSYLKHFEHCSKTPSDVNCFAKYGGPIDDISLVLGGFNGSDFHLASALVITIPVNNFNDVEKKYPALAWEKEFVKLMKNYNNTEVMTVAFMAERSIEDELERGSHSDVVTVGISYVIMFAYIAIALGDINSCSRLLIDSKISLGLVGVIIVLLSVVASLGIFSFFGVSATLIIVEVIPFLVLAVGVDNIFILVQQFQRDVRREGETTVEQVGRLVGEVAPSMMLSSVSMSACFFIGALTETPAVRIFALYAGVALLINFFLQMTCFLSLFTLDTLRQEDGRLDLCFCIRASKKSRPSQNTSLLYKFFKKVYAPFLLNNSVRVVVMITFIGWLCSSLAVIGKIEVGLDQELAMPKDSYLQQYFDYLKKYLQVGPPVYFMVTEGYDYSKVENQAKLCIQEQVCDQDSVGAKLKQLTLLSNRTYVTRLRSYWLDQYILYMRSSGCCYEGQNSSDFCYSQYGGGKCKSCHVPREKPFVGEEFLHYLSWFLKDVPGAKCSSAGRAEHGGSIDHVNGTIKSAYFSAYHPVLKTSKDFYTALDWARLISHNLTQDIQAIQPGVQVIPYSLVHVFYEQYLTMWPDTFKNLALSLGAIFVVTFVLLGLDFVSATVVTFTIVMIIVNLMGLMYWWDISLNAVSLVNLVVGVGISVEFCSHLVRVFALSGAPSRVKRAQDALTKMGSSILSGITLTDCGILVLAFAKSQIFQVFYFRMYLGIIAFGTLHSLIFLPVFLSILGPPVNKDKLIEHIQLQEMATVTTAVVRSKADTYTRKDVSA

Target ORF information:

RefSeq Version	XM_002435602.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis Niemann-Pick type C1 domain-containing protein, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002435602.1

ORF Insert Sequence:

ATGAAGGCCG AGCTGGAACA GCCCCTCGGC CTGGGCATGG GCAAGTGTCC CACGTGCTAC 
AGCAACTTTG TCCGCATCTT TTGCGGCTTT TGCGACCCAA ACCACGCCGA CTACATCGCC 
ATCAACAGGA CCGAGCCGAG TGACGAGCAG GAAGGGAAGG AGACGGTCCT GGCCGTGGAC 
TACGCCGTCA GCAAAGAGTT TGCGCACGGG GCCTTCGACT CGTGTGCCCA CGTGCAGTCG 
GTGGTGACGG ACAACACGGT GATGCAGTTC ATGTGCGGTA GCAAGGGCAA GGACTGCACG 
GCGGCCGACT GGTTCACCTT CCTGGGAAGC ACCTCCGACG AGAACGGCTT TGCTCCGCTC 
AAGTTCAACT ACATCATCAC GGGGGAGAGG AGCATTACGG CCAGCGGCGT TTCCCTCAAG 
CCGTTCAACC CAGGCCACCA TCGATGCTCG GAGCCGTTTG GAGCCAGCAA GCAGCGGTGC 
AGTTGCAGCG ACTGCCCGGA GGTTTGCGTG GCCTTGGAGC CACCGCTGTT GCCACCGGAC 
GCGATGCCTT TCACCATTGG TCGCTACGAC GGGATGCTGG TGGTTTCCAT GCTCCTCTTT 
GTGCTCCTCA GCGCCGGCGT CCTCGGAGTT TTCTTCCTCA AGAGCAACAG GAGGCGGAGC 
TCGTTCAGAG TCAGCTCCAA CGGCTTCACC AACGCCTCGG CCGTGGACTC CCCGACACCA 
TCTCCTCCCG GCTCTCCTAT CTCGCTCCGG CCGCCGCCGC TGTCCGGAGA CGCGGTCAAC 
AACGGCAGCG TCGCCAACGG CAACGCCAAC GGCAGCGCGG TGAAGCCCCC CGCGGAGGAG 
CCGCACGTCC GCACGCTCTC GTCGTTCGGG GCCACCATGG AACAACTGCT GCAGGCCGGC 
TTCCACCGCT GGGGCCTCTT TGTTGCCCGC AACCCCCTCC TGGTGCTCAT CGCCGCGCTG 
GCTGTGAGCG TCCTCCTGTC TCTGGGTCTG CTCAAGTTTA CTGTGACTAC GAACCCCGTG 
GACCTGTGGG TGTCACACAG CAGTCTGGCC AGGAAACACA TGAACTACTT CAACAGTCAC 
TTTGGTCCTT TCTACCGGGT GGAGCAGATA ATCCTCCGTC CCAAGAACCA GCAGTTCTTT 
GTGCTGGAGA CGGACGGAGT AAACAGAACA TTTGGGCCGG CATTCGAGAA GAATTTTATG 
ATGGAGGTGG GTTATAAGCT TTTGTACCAC CTACAGGTTT TGGATCCGAA GGACGTGAAG 
AACGTCACCA TTCAGGACGT CTGCCTGTCC CCACTGTCCC CGCTGAACAG GCATTGTTCG 
GTGCAGAGCA TCTTCGCCTA CTACCAAGAC GACCCGTCCA AGCTCAACCT GACGGACAAG 
TTGGATCCGC TCTCTTACCT CAAGCACTTC GAACACTGCA GCAAAACTCC GTCTGACGTC 
AACTGCTTCG CCAAGTATGG AGGACCGATC GATGACATCT CCCTGGTTCT GGGGGGATTC 
AATGGGAGTG ACTTTCACCT GGCCTCTGCT CTGGTAATCA CCATACCGGT GAACAACTTC 
AACGACGTAG AGAAGAAGTA CCCTGCCCTC GCCTGGGAGA AAGAGTTCGT CAAGCTCATG 
AAGAACTACA ACAACACCGA GGTCATGACT GTTGCCTTCA TGGCTGAGAG ATCTATCGAA 
GACGAACTGG AGCGAGGCAG TCACTCTGAC GTCGTGACGG TTGGCATCAG CTACGTCATC 
ATGTTCGCCT ACATCGCCAT TGCGCTGGGC GACATCAACA GTTGCAGTAG ACTTTTGATT 
GACTCAAAGA TTTCCCTGGG CCTGGTTGGA GTGATAATTG TTCTTCTGTC CGTGGTGGCC 
TCGCTGGGCA TCTTCAGCTT CTTTGGCGTT TCTGCCACGC TCATCATTGT TGAAGTGATC 
CCCTTCCTGG TGCTTGCTGT TGGGGTCGAC AACATTTTCA TCCTGGTGCA GCAGTTTCAG 
CGCGATGTCC GTCGCGAGGG AGAGACAACG GTGGAGCAGG TGGGGAGGCT GGTAGGAGAG 
GTTGCCCCAA GCATGATGCT CTCCAGTGTC TCCATGTCTG CCTGCTTCTT CATCGGTGCC 
TTGACAGAAA CTCCCGCAGT GCGGATCTTT GCGCTGTACG CCGGTGTCGC CCTGCTCATC 
AACTTCTTCC TACAAATGAC TTGCTTCCTC TCCTTGTTTA CTCTGGACAC ACTTAGGCAG 
GAGGACGGCC GTCTAGATCT GTGCTTCTGC ATCCGTGCTT CAAAGAAGAG CCGTCCGTCC 
CAAAACACCA GTCTGCTGTA CAAGTTCTTC AAGAAAGTCT ACGCACCCTT CTTGCTGAAC 
AACTCCGTGC GTGTGGTGGT GATGATCACG TTCATTGGCT GGCTCTGTTC GTCCCTCGCC 
GTCATCGGAA AAATTGAAGT TGGGCTGGAT CAGGAGCTGG CCATGCCTAA GGATTCCTAC 
CTTCAGCAGT ACTTTGACTA CCTCAAGAAG TACCTCCAAG TAGGACCGCC GGTGTACTTC 
ATGGTCACGG AGGGCTATGA CTACTCCAAG GTGGAGAACC AGGCAAAACT CTGCATCCAG 
GAGCAAGTGT GTGATCAGGA CTCTGTGGGT GCTAAGTTGA AGCAGCTCAC ACTGCTCAGC 
AACAGGACTT ACGTGACGAG GTTACGGTCT TACTGGTTGG ACCAGTACAT CCTTTACATG 
CGGTCGTCCG GCTGTTGCTA CGAGGGCCAG AACAGCTCAG ACTTCTGCTA TTCGCAGTAT 
GGGGGTGGAA AATGCAAAAG TTGTCACGTT CCGAGGGAGA AGCCCTTCGT AGGAGAGGAG 
TTTTTGCATT ACCTCTCTTG GTTCCTGAAG GACGTACCGG GCGCCAAGTG TTCTTCAGCA 
GGTCGCGCGG AACATGGCGG TTCGATTGAT CACGTAAACG GAACTATTAA ATCTGCCTAC 
TTTTCGGCCT ACCACCCTGT CCTGAAGACG TCTAAGGACT TCTACACGGC ACTCGACTGG 
GCTCGGCTCA TCTCCCACAA CCTGACCCAG GACATACAGG CCATTCAGCC AGGAGTGCAA 
GTCATCCCTT ATAGCCTGGT GCACGTGTTC TACGAGCAGT ACCTCACCAT GTGGCCGGAC 
ACGTTCAAGA ACCTGGCCCT GTCGCTGGGT GCCATATTTG TGGTCACCTT TGTGCTGCTG 
GGGCTGGACT TTGTGTCTGC GACGGTGGTC ACCTTCACCA TCGTCATGAT CATCGTCAAC 
CTGATGGGCC TGATGTACTG GTGGGACATC TCGCTCAACG CGGTGTCCCT CGTCAACCTC 
GTGGTGGGCG TGGGGATCTC GGTGGAGTTC TGCTCCCACC TGGTGCGAGT GTTTGCTCTC 
AGCGGTGCCC CGTCCCGAGT CAAAAGGGCC CAGGACGCAC TCACCAAGAT GGGCAGTTCG 
ATCCTGTCCG GAATCACGCT GACGGATTGT GGCATCCTGG TGCTAGCCTT TGCCAAGTCG 
CAGATATTCC AGGTTTTCTA TTTCCGCATG TACCTGGGCA TCATTGCCTT TGGCACGCTG 
CACAGCCTCA TCTTCCTCCC GGTGTTTCTA AGCATCCTGG GTCCCCCCGT CAACAAGGAC 
AAGTTGATAG AGCACATTCA GCTCCAGGAG ATGGCCACAG TCACTACGGC CGTCGTGAGG 
TCCAAGGCAG ACACCTACAC TCGAAAGGAC GTCAGCGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IscW_ISCW006533 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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