LOC100875902 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100875902
Entrez Gene ID	100875902
Full Name	protein enabled
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012284484.1	XP_012139874.1	protein enabled

Pathways from BioSystems
Gene Ontology

The following LOC100875902 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100875902 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe103950	XM_012284484.1 Latest version!	Megachile rotundata protein enabled (LOC100875902), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100875902 ( XM_012284484.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012139874.1 Megachile rotundata protein enabled (LOC100875902), mRNA.

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CloneID	OMe103950
Clone ID Related Accession (Same CDS sequence)	XM_012284484.1
Accession Version	XM_012284484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	protein enabled
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797166.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATACCAA CGAACGACGC AAGGCGATTC ATGCATCGCC TTCAACCGCA ACGATCCCTT 
CCGGAAGAAT ATCGATCGCG CGAAGTGAGC ATATCGTCCG CTCGTGCGTC GGTAATGGTA 
TACGACGACG TTAACAAAAA ATGGGTACCG AGCGGCAGTT CGTCAGGACT TTCGAAAGTT 
CAATTGTACC ATCATCAAGT GAACAATACG TTTCGCGTGG TTGGAAGAAA GTTGCAGGAT 
CACGAAATCG TCATCAACTG CGCGATCCTC AAGGGACTGA AATACAATCA AGCGACAGTC 
ACGTTTCATC AATGGCGAGA TAACAAGCAA GTATACGGTC TGAATTTCTC CAGTAAGGAC 
GACGCCGACG CATTTGCCAG AGCGATGCTG CAAGCGCTCG ATGTGTTGAG TAATGGAAGC 
AACATATCGA GGAGCCTCGC ACCCGCATCG ACTACTCAGC AGCAGCCAGT TTTTCAGCAA 
CAACAACCGA CTAATGCTCA ATACGATGAA GATATGGGAT ACAGAACTAT GACCAGGGAG 
GATGTGGCAA TCATTCAGGA ACGCAGAATG TCTCAACAAA GTCAAGCAAC GAACAGTCCG 
AACGCAATTT CTCCCAGCTG TCCTCTCGCG TCGCAGCAGC AACAAGTACC TCCGCAACCG 
CAGCAACAGC CGCAGCAACC GGCTGCGCAA TCCGTGCAAC AACAACAGCA ACAACAACCG 
ACGGCCCCGC CTCAACCTCC GCAACCGCCA CAGCAGCAAC AGCATCCGAT ACAGCAACCG 
CAGCAACAAC CGTTACAGCA GCAACCGCAG TCGGGTCACC ACAGAACTTC GTCGGCTCCA 
CCGGCTCCGC AGCCTCAACA ACAAACCGTG ATGCAATCGA TGCAAGTAAC CGGTGGTTTG 
GGAACCGGTG CTGGACAAAT GTCGAGTGGA AGCGTGCCAC CGGTACCACC TCCTCAGCCT 
CCTATGTCCA ACGCGGTTCC GCCGTGTCCA CCCGCCATGA TGAACTTTAA CGTGCCGGTA 
CCACCACCTC CGATGATGAT GAACCAGTAC GCGCAGTCCC CGTCGTCTCA ATCTAATCTA 
CCGAATCAAA CACAAGCACA GGCGATCTAC GGTAGCGGTC AAACGAATCA GTACGGTGTC 
GCCGCAAGCA ATAACCAGTA CGCGACCGGT GCCGTGATCG GGCAAAGTCC GAGTCAGTAT 
TCTACCGGCA ATCAAAATAA TTTCTACGGT AACAATGGCC AAGTTAGTAA CGCCTATTCG 
AGCGGACAAT CGGCACAGGC GAATCAGTAC GGAGGCGGCG GAGGTAGTGG TGGTACTGGT 
AGTGCTGGTG GAAGCCAATA CGGAAGCAGC AATTCGATCG GTCAATACGC CACCAGTGCA 
GCAGCAGCAG CAACCGGCCA GTACGCCGTA GGTGTCGGTG TCAATGTCGG CGTCGGCGTT 
GGCCAACCGA ATCAAAATCA GTACAGCGTG GTGTCTCAGG CACAGGCACA GTATGGTACG 
GGTCAGATAC AAGCAGTTTC GGGTGCGACA AATCCGTATG GGACACCCTC GAATTCGGTG 
AATCAGTATA ACAGTAGCGG TGGCGGTAAT AACAGTGGTA ATTGCGGTGC TGGCGGCGGC 
GGTGGCGGTG GTGGTGGCAG CGGTGGTAAC AGTGGTACCG CTGCTGCTCA TCTTGTACCT 
TCGGCCAATC CTAATATTTA TGCCGCTGTC GAGCAACAGC ATCCGATACA GCAACCGCAG 
CAACAACCGT TACAGCAGCA ACCGCAGTCG GGTCACCACA GAACTTCGTC GGCTCCACCG 
GCTCCGCAGC CTCAACAACA AACCGTGATG CAATCGATGC AAGTAACCGG TGGTTTGGGA 
ACCGGTGCTG GACAAATGTC GAGTGGAAGC GTGCCACCGG TACCACCTCC TCAGCCTCCT 
ATGTCCAACG CGGTTCCGCC GTGTCCACCC GCCATGATGA ACTTTAACGT GCCGGTACCA 
CCACCTCCGA TGATGATGAA CCAGTACGCG CAGTCCCCGT CGTCTCAATC TAATCTACCG 
AATCAAACAC AAGCACAGGC GATCTACGGT AGCGGTCAAA CGAATCAGTA CGGTGTCGCC 
GCAAGCAATA ACCAGTACGC GACCGGTGCC GTGATCGGGC AAAGTCCGAG TCAGTATTCT 
ACCGGCAATC AAAATAATTT CTACGGTAAC AATGGCCAAG TTAGTAACGC CTATTCGAGC 
GGACAATCGG CACAGGCGAA TCAGTACGGA GGCGGCGGAG GTAGTGGTGG TACTGGTAGT 
GCTGGTGGAA GCCAATACGG AAGCAGCAAT TCGATCGGTC AATACGCCAC CAGTGCAGCA 
GCAGCAGCAA CCGGCCAGTA CGCCGTAGGT GTCGGTGTCA ATGTCGGCGT CGGCGTTGGC 
CAACCGAATC AAAATCAGTA CAGCGTGGTG TCTCAGGCAC AGGCACAGTA TGGTACGGGT 
CAGATACAAG CAGTTTCGGG TGCGACAAAT CCGTATGGGA CACCCTCGAA TTCGGTGAAT 
CAGTATAACA GTAGCGGTGG CGGTAATAAC AGTGGTAATT GCGGTGCTGG CGGCGGCGGT 
GGCGGTGGTG GTGGCAGCGG TGGTAACAGT GGTACCGCTG CTGCTCATCT TGTACCTTCG 
GCCAATCCTA ATATTTATGC CGCTGTCGGC AGTGCCGGTC CGCAACCACC ACCTATGCTA 
CCATTAGCTG GTCCTGCAGC ACCACCTCCA CCACCTCCGC CACCTGCACC AAAAATCAAC 
GCTGTCAACC TCAATCCGCT TTCTGGGCCT GCATCAACTA CTACTACTAC CACTACTACT 
AATCTCGTCA GTTCTGCTAG CAACAACGAC CCGCCTCCAG ACTCCAATTC GTTGGCTGCT 
GCTCTTCAAG CGGCTCGTTT GAAAAAGAAG CAGTCGTCGC AACAGCCGGT GGAGAATAGC 
GGTTCGAGTA CCAGTAGCAG TGGAAGCGCA GGAAGCAGTG GAAATTATGG CACGTTAGGA 
AGAGGCGGAG GTGGCGGTAT GGCTTCCATG ATGGACGAGA TGGCTAAAAC TTTGGCTCGT 
AGACGAGCTG CCGTCGAGAA GAAGCAACCC GAACAGCCAC AGGAACCCGA AAGTTCACCC 
GATAAAAAAT CATGGGACAA GAACAATTCG AATAATAAAT TTTCGAATGG CGCCGAATCA 
CCGAAGTCTG TCCGCAAAAG GTTTGGTTCC GCATCCGAGG ATACTCTCTT GAAAGTAAAC 
GGCGTTAACG ATGGAGCTGT CCTCACTGCT CAAGAAATGG AGGCGTTCAA AGCCGAGATC 
ATCAAGGAAG TACGCAAAGA ATTTCAAAAG ATGAAGCAAG ATATCGTGGA CGCGGTCAGA 
GCGGAACTCA GTAGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012139874.1
CDS	501..3881
Translation	MIPTNDARRFMHRLQPQRSLPEEYRSREVSISSARASVMVYDDVNKKWVPSGSSSGLSKVQLYHHQVNNTFRVVGRKLQDHEIVINCAILKGLKYNQATVTFHQWRDNKQVYGLNFSSKDDADAFARAMLQALDVLSNGSNISRSLAPASTTQQQPVFQQQQPTNAQYDEDMGYRTMTREDVAIIQERRMSQQSQATNSPNAISPSCPLASQQQQVPPQPQQQPQQPAAQSVQQQQQQQPTAPPQPPQPPQQQQHPIQQPQQQPLQQQPQSGHHRTSSAPPAPQPQQQTVMQSMQVTGGLGTGAGQMSSGSVPPVPPPQPPMSNAVPPCPPAMMNFNVPVPPPPMMMNQYAQSPSSQSNLPNQTQAQAIYGSGQTNQYGVAASNNQYATGAVIGQSPSQYSTGNQNNFYGNNGQVSNAYSSGQSAQANQYGGGGGSGGTGSAGGSQYGSSNSIGQYATSAAAAATGQYAVGVGVNVGVGVGQPNQNQYSVVSQAQAQYGTGQIQAVSGATNPYGTPSNSVNQYNSSGGGNNSGNCGAGGGGGGGGGSGGNSGTAAAHLVPSANPNIYAAVEQQHPIQQPQQQPLQQQPQSGHHRTSSAPPAPQPQQQTVMQSMQVTGGLGTGAGQMSSGSVPPVPPPQPPMSNAVPPCPPAMMNFNVPVPPPPMMMNQYAQSPSSQSNLPNQTQAQAIYGSGQTNQYGVAASNNQYATGAVIGQSPSQYSTGNQNNFYGNNGQVSNAYSSGQSAQANQYGGGGGSGGTGSAGGSQYGSSNSIGQYATSAAAAATGQYAVGVGVNVGVGVGQPNQNQYSVVSQAQAQYGTGQIQAVSGATNPYGTPSNSVNQYNSSGGGNNSGNCGAGGGGGGGGGSGGNSGTAAAHLVPSANPNIYAAVGSAGPQPPPMLPLAGPAAPPPPPPPPAPKINAVNLNPLSGPASTTTTTTTTNLVSSASNNDPPPDSNSLAAALQAARLKKKQSSQQPVENSGSSTSSSGSAGSSGNYGTLGRGGGGGMASMMDEMAKTLARRRAAVEKKQPEQPQEPESSPDKKSWDKNNSNNKFSNGAESPKSVRKRFGSASEDTLLKVNGVNDGAVLTAQEMEAFKAEIIKEVRKEFQKMKQDIVDAVRAELSRR

Target ORF information:

RefSeq Version	XM_012284484.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata protein enabled (LOC100875902), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012284484.1

ORF Insert Sequence:

ATGATACCAA CGAACGACGC AAGGCGATTC ATGCATCGCC TTCAACCGCA ACGATCCCTT 
CCGGAAGAAT ATCGATCGCG CGAAGTGAGC ATATCGTCCG CTCGTGCGTC GGTAATGGTA 
TACGACGACG TTAACAAAAA ATGGGTACCG AGCGGCAGTT CGTCAGGACT TTCGAAAGTT 
CAATTGTACC ATCATCAAGT GAACAATACG TTTCGCGTGG TTGGAAGAAA GTTGCAGGAT 
CACGAAATCG TCATCAACTG CGCGATCCTC AAGGGACTGA AATACAATCA AGCGACAGTC 
ACGTTTCATC AATGGCGAGA TAACAAGCAA GTATACGGTC TGAATTTCTC CAGTAAGGAC 
GACGCCGACG CATTTGCCAG AGCGATGCTG CAAGCGCTCG ATGTGTTGAG TAATGGAAGC 
AACATATCGA GGAGCCTCGC ACCCGCATCG ACTACTCAGC AGCAGCCAGT TTTTCAGCAA 
CAACAACCGA CTAATGCTCA ATACGATGAA GATATGGGAT ACAGAACTAT GACCAGGGAG 
GATGTGGCAA TCATTCAGGA ACGCAGAATG TCTCAACAAA GTCAAGCAAC GAACAGTCCG 
AACGCAATTT CTCCCAGCTG TCCTCTCGCG TCGCAGCAGC AACAAGTACC TCCGCAACCG 
CAGCAACAGC CGCAGCAACC GGCTGCGCAA TCCGTGCAAC AACAACAGCA ACAACAACCG 
ACGGCCCCGC CTCAACCTCC GCAACCGCCA CAGCAGCAAC AGCATCCGAT ACAGCAACCG 
CAGCAACAAC CGTTACAGCA GCAACCGCAG TCGGGTCACC ACAGAACTTC GTCGGCTCCA 
CCGGCTCCGC AGCCTCAACA ACAAACCGTG ATGCAATCGA TGCAAGTAAC CGGTGGTTTG 
GGAACCGGTG CTGGACAAAT GTCGAGTGGA AGCGTGCCAC CGGTACCACC TCCTCAGCCT 
CCTATGTCCA ACGCGGTTCC GCCGTGTCCA CCCGCCATGA TGAACTTTAA CGTGCCGGTA 
CCACCACCTC CGATGATGAT GAACCAGTAC GCGCAGTCCC CGTCGTCTCA ATCTAATCTA 
CCGAATCAAA CACAAGCACA GGCGATCTAC GGTAGCGGTC AAACGAATCA GTACGGTGTC 
GCCGCAAGCA ATAACCAGTA CGCGACCGGT GCCGTGATCG GGCAAAGTCC GAGTCAGTAT 
TCTACCGGCA ATCAAAATAA TTTCTACGGT AACAATGGCC AAGTTAGTAA CGCCTATTCG 
AGCGGACAAT CGGCACAGGC GAATCAGTAC GGAGGCGGCG GAGGTAGTGG TGGTACTGGT 
AGTGCTGGTG GAAGCCAATA CGGAAGCAGC AATTCGATCG GTCAATACGC CACCAGTGCA 
GCAGCAGCAG CAACCGGCCA GTACGCCGTA GGTGTCGGTG TCAATGTCGG CGTCGGCGTT 
GGCCAACCGA ATCAAAATCA GTACAGCGTG GTGTCTCAGG CACAGGCACA GTATGGTACG 
GGTCAGATAC AAGCAGTTTC GGGTGCGACA AATCCGTATG GGACACCCTC GAATTCGGTG 
AATCAGTATA ACAGTAGCGG TGGCGGTAAT AACAGTGGTA ATTGCGGTGC TGGCGGCGGC 
GGTGGCGGTG GTGGTGGCAG CGGTGGTAAC AGTGGTACCG CTGCTGCTCA TCTTGTACCT 
TCGGCCAATC CTAATATTTA TGCCGCTGTC GAGCAACAGC ATCCGATACA GCAACCGCAG 
CAACAACCGT TACAGCAGCA ACCGCAGTCG GGTCACCACA GAACTTCGTC GGCTCCACCG 
GCTCCGCAGC CTCAACAACA AACCGTGATG CAATCGATGC AAGTAACCGG TGGTTTGGGA 
ACCGGTGCTG GACAAATGTC GAGTGGAAGC GTGCCACCGG TACCACCTCC TCAGCCTCCT 
ATGTCCAACG CGGTTCCGCC GTGTCCACCC GCCATGATGA ACTTTAACGT GCCGGTACCA 
CCACCTCCGA TGATGATGAA CCAGTACGCG CAGTCCCCGT CGTCTCAATC TAATCTACCG 
AATCAAACAC AAGCACAGGC GATCTACGGT AGCGGTCAAA CGAATCAGTA CGGTGTCGCC 
GCAAGCAATA ACCAGTACGC GACCGGTGCC GTGATCGGGC AAAGTCCGAG TCAGTATTCT 
ACCGGCAATC AAAATAATTT CTACGGTAAC AATGGCCAAG TTAGTAACGC CTATTCGAGC 
GGACAATCGG CACAGGCGAA TCAGTACGGA GGCGGCGGAG GTAGTGGTGG TACTGGTAGT 
GCTGGTGGAA GCCAATACGG AAGCAGCAAT TCGATCGGTC AATACGCCAC CAGTGCAGCA 
GCAGCAGCAA CCGGCCAGTA CGCCGTAGGT GTCGGTGTCA ATGTCGGCGT CGGCGTTGGC 
CAACCGAATC AAAATCAGTA CAGCGTGGTG TCTCAGGCAC AGGCACAGTA TGGTACGGGT 
CAGATACAAG CAGTTTCGGG TGCGACAAAT CCGTATGGGA CACCCTCGAA TTCGGTGAAT 
CAGTATAACA GTAGCGGTGG CGGTAATAAC AGTGGTAATT GCGGTGCTGG CGGCGGCGGT 
GGCGGTGGTG GTGGCAGCGG TGGTAACAGT GGTACCGCTG CTGCTCATCT TGTACCTTCG 
GCCAATCCTA ATATTTATGC CGCTGTCGGC AGTGCCGGTC CGCAACCACC ACCTATGCTA 
CCATTAGCTG GTCCTGCAGC ACCACCTCCA CCACCTCCGC CACCTGCACC AAAAATCAAC 
GCTGTCAACC TCAATCCGCT TTCTGGGCCT GCATCAACTA CTACTACTAC CACTACTACT 
AATCTCGTCA GTTCTGCTAG CAACAACGAC CCGCCTCCAG ACTCCAATTC GTTGGCTGCT 
GCTCTTCAAG CGGCTCGTTT GAAAAAGAAG CAGTCGTCGC AACAGCCGGT GGAGAATAGC 
GGTTCGAGTA CCAGTAGCAG TGGAAGCGCA GGAAGCAGTG GAAATTATGG CACGTTAGGA 
AGAGGCGGAG GTGGCGGTAT GGCTTCCATG ATGGACGAGA TGGCTAAAAC TTTGGCTCGT 
AGACGAGCTG CCGTCGAGAA GAAGCAACCC GAACAGCCAC AGGAACCCGA AAGTTCACCC 
GATAAAAAAT CATGGGACAA GAACAATTCG AATAATAAAT TTTCGAATGG CGCCGAATCA 
CCGAAGTCTG TCCGCAAAAG GTTTGGTTCC GCATCCGAGG ATACTCTCTT GAAAGTAAAC 
GGCGTTAACG ATGGAGCTGT CCTCACTGCT CAAGAAATGG AGGCGTTCAA AGCCGAGATC 
ATCAAGGAAG TACGCAAAGA ATTTCAAAAG ATGAAGCAAG ATATCGTGGA CGCGGTCAGA 
GCGGAACTCA GTAGAAGATA A