ORF cDNA Clones and Custom Clones » Species Summary » Mus musculus » Phka2 cDNA ORF clone

Phka2 cDNA ORF clone, Mus musculus(house mouse)

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Summary

Gene Symbol	Phka2
Entrez Gene ID	110094
Full Name	phosphorylase kinase alpha 2
Synonyms	6330505C01Rik,D330034O08,Phk
General protein information	Preferred Names phosphorylase kinase alpha 2 Names phosphorylase b kinase regulatory subunit alpha, liver isoform phosphorylase kinase alpha L subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F4\|X 73.95 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006528680.3	XP_006528743.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
NM_001177878.1	NP_001171349.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform b
XM_006528682.1	XP_006528745.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
NM_172783.3	NP_766371.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
NM_172783.4	NP_766371.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
NM_001358968.1	NP_001345897.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform d
NM_001177879.1	NP_001171350.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
XM_011247771.1	XP_011246073.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
XM_006528679.4	XP_006528742.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_030251193.1	XP_030107053.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
NM_001177879.2	NP_001171350.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
NM_001177878.2	NP_001171349.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform b
XM_006528679.3	XP_006528742.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
NM_001358966.1	NP_001345895.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform c
XM_006528681.1	XP_006528744.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_030251194.1	XP_030107054.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3

Homologies

	PHKA2	XP_001084454.1
Rattus norvegicus (Norway rat)	Phka2	NP_001177923.1
Danio rerio (zebrafish)	LOC556199	XP_684044.4
Drosophila melanogaster (fruit fly)	CG7766	NP_001138180.1
Pan troglodytes (chimpanzee)	PHKA2	XP_003317431.1
Mus musculus (house mouse)	Phka2	NP_001171350.1
Homo sapiens (human)	PHKA2	NP_000283.1
Bos taurus (cattle)	PHKA2	NP_001178474.1
Canis lupus familiaris (dog)	PHKA2	XP_005641224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000708	XP_559348.4
Caenorhabditis elegans (roundworm)	C14B9.8	NP_498777.2
Gallus gallus (chicken)	PHKA2	XP_004934744.1
Xenopus tropicalis (tropical clawed frog)	phka2	XP_004911720.1

Bibliography

Related articles in PubMed

The apical ectodermal ridge of the mouse model of ectrodactyly Dlx5;Dlx6-/- shows altered stratification and cell polarity, which are restored by exogenous Wnt5a ligand.
Conte D, Garaffo G, Lo Iacono N, Mantero S, Piccolo S, Cordenonsi M, Perez-Morga D, Orecchia V, Poli V, Merlo GR
Human molecular genetics25(4)740-54(2016 Feb)

Glycine N-methyltransferase-/- mice develop chronic hepatitis and glycogen storage disease in the liver.
Liu SP, Li YS, Chen YJ, Chiang EP, Li AF, Lee YH, Tsai TF, Hsiao M, Huang SF, Hwang SF, Chen YM
Hepatology (Baltimore, Md.)46(5)1413-25(2007 Nov)

GenePaint.org: an atlas of gene expression patterns in the mouse embryo.
Visel A, Thaller C, Eichele G
Nucleic acids research32(Database issue)D552-6(2004 Jan)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Comparative FISH mapping of mouse and rat homologues of twenty-five human X-linked genes.
Kuroiwa A, Watanabe T, Hishigaki H, Takahashi E, Namikawa T, Matsuda Y
Cytogenetics and cell genetics81(3-4)208-12(1998)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following Phka2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phka2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83675	NM_001358968.1 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	10-12	$307.30 $439.00
OMu72326	XM_011247771.1 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X7, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	12-14	$391.30 $559.00
OMu56572	XM_006528681.1 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	16	$643.30 $919.00
OMu56573	XM_006528682.1 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X6, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	16	$559.30 $799.00
OMu13343	NM_172783.4 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
OMu13343	NM_001177879.2 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
OMu83674	NM_001358966.1 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 4, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	19-21	$643.30 $919.00
OMu56570	XM_006528679.4 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	19-21	$643.30 $919.00
OMu56573	XM_030251193.1 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X5, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	19-21	$559.30 $799.00
OMu56573	XM_030251194.1 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X6, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	19-21	$559.30 $799.00
OMu13378	NM_001177878.2 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
OMu56571	XM_006528680.3 Latest version!	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu13343	NM_172783.3	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
OMu13343	NM_001177879.1	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 2, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
OMu13378	NM_001177878.1	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 3, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	20	$643.30 $919.00
OMu56570	XM_006528679.3	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X1, mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	16	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phka2 ( NM_001358968.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345897.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu83675
Clone ID Related Accession (Same CDS sequence)	NM_001358968.1
Accession Version	NM_001358968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731801.11 and BC058254.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058254.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGG TAAAAAGAAC AGGCATGGGG GCCCGTGCTA GTGCATGCCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345897.1
CDS	666..2279
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQVKRTGMGARASACL

Target ORF information:

RefSeq Version	NM_001358968.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358968.1

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGG TAAAAAGAAC AGGCATGGGG GCCCGTGCTA GTGCATGCCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_011247771.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246073.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu72326
Clone ID Related Accession (Same CDS sequence)	XM_011247771.1
Accession Version	XM_011247771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTAT TTTGCCCAGA CTGGCTTCAA ACTTTCTATG GAGCTGAAGG TGGCCCCAAC 
CTTCTGATCC TCCTGCTCCT ATTTCCCAAG TGCTCACATT ACAGAGTAAA ACTAGGAAAT 
CTGGCAGAGT TCCTTACTAC CTCATTCTAC ACACACCTGA CCTTCCTGGA TCCAGACTGC 
GATGAGAAGT TGTTTGGTGA CATCACTGAT AGGAGCTTTA GTCCTGACAG TGAGCCAGAC 
CTGGGAGGAT ACCTGGAAGA CAGCAGTCCT CAAGAAAGCC AAGATGAACT TGACCAGTAT 
ATCAGCCACC TTCTTCAAAG CACATCCTTG AAGTGTTACC TGCCCCCTCT TTGTAAGAAG 
TCAGAAGACA GCCATTTTTT CAGTGCTATT CACTCCACTC GGGACATACT TTCTGTGATG 
GCCAAAGCGA AGGGTTTGGA AACTACATTT TTTCCCATGA TTTTGCCAAC TAAAGTTCTA 
AGTGGACACC GTAAGTCACT GAATCTTGTT GACTCCCCTC AGCCACTCCT AAAGACGACT 
CCTGAATATG ACTACCAGTG GCCCAGAGAC GACCATGATG AAGTGGACTG TGAGAAGCTA 
GTTGGGCAAC TGAAAGACTG CTCAAACCTA CAGGACCAAG CAGACATTCT GTACATTCTT 
TATGTAATGA AGGGTCCCCG CTGGGATACC AATTTGTTTG GGCAGCATGG AGTCACTGTT 
CACAGTCTTC TCAGCGAGCT CTATGGAAAA GCTGGCCTAA ACCAAGAATG GAGTTTGATT 
CGCTACATTT CAGGCCTGCT CAGGAAGAAA GTGGAGGTCC TGGCTGAGGC CTGTGCAGAT 
CTGCTGTCCC ACCAGAAGCA GCTTACAGTA GGACTGCCCC CTGAGCCCCG GGAGAAGACC 
ATATCTACGC CTCTACCCCC AGAGGAGCTC ACAGAACTCA TATATGAAGC CAGTGGACAG 
GACATCAGCA TTGCTGTCCT CACACAGGAG ATCGTGGTTT ACCTGGCCAT GTATGTCCGG 
GCCCAGCCTA GCCTCTTTGC AGAGATGCTC AGACTCCGGA TTGGATTGAT CATCCAGGTG 
ATGGCCACAG AGCTGGCTCG GAGCCTGAAC TGCTCAGGAA AAGAAGCTTC GGAGAGCTTG 
ATGAACCTCA GCCCTTTCGA CATGAAGAGC CTTCTGCATC ATATCCTGAG TGGGAAGGAG 
TTCGGTGTGG AGAGAAGCGT GCGCCCAATA CACTCCTCCA TGTCCAGCCC TGCCATCTCC 
ATCCATGAGG TGGGTCATAC TGGAGCCACC AAAACTGAGC GGAGTGGCAT CACCAGACTG 
AGGAGTGAGA TGAAACAGAT GAATAGGCGA GCTAGTGCTG ATGAGCAGTT CTTTCCTTTG 
GGCCAGACCA TGTCCAACAG TTTGCATTCC ATCAAGTCTG TGAGGTCCAG CACCCCATCC 
TCCCCGACAG GCACATCATC TACAGACTCT GGAGGACAGC ACCTGGGCTG GGGAGAACAG 
CAGGGCCAGT GGCTGCGCCG GAGAAGGTTG GATGGGGCCA TTAACAGGGT CCCCGTGGGA 
TTCTACCAGA AAGTGTGGAA GATCCTTCAG AAGTGCCATG GCCTGTCCAT CGATGGTTAC 
GTCCTCCCAT CCTCGACAAC CCAAGAGATG ACCCCTTGTG AGATCAAGTT TGCCGTCCAT 
GTGGAGTCAG TGCTCAACCG TGTGTCCCAG CCTGAGTATC GGCAGCTACT GGTAGAAGCC 
ATCATGGTAC TGACTCTGCT CTCAGATACC GAAATGGACA GTATCGGGGG CATCATCCAT 
GTGGACCAGA TAGTGCAGCT GGCCAATCAG CTGTTCCTGC AGGACCAGGT GTCATTTGGA 
ACCACAGACA TCCTGGAAAA AGACCAAGCC ACAGGAATTT GCCACCTCTT TTATGACAGT 
GCTCCTAGTG GGGCTTATGG TACAATGACC TACCTAACAA AAGCGGTCGC TTCTCATTTG 
CAGGAACTGC TGCCCAGTTC AGGCTGCCAG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246073.1
CDS	272..2290
Translation	MVLFCPDWLQTFYGAEGGPNLLILLLLFPKCSHYRVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	XM_011247771.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247771.1

ORF Insert Sequence:

ATGGTCTTAT TTTGCCCAGA CTGGCTTCAA ACTTTCTATG GAGCTGAAGG TGGCCCCAAC 
CTTCTGATCC TCCTGCTCCT ATTTCCCAAG TGCTCACATT ACAGAGTAAA ACTAGGAAAT 
CTGGCAGAGT TCCTTACTAC CTCATTCTAC ACACACCTGA CCTTCCTGGA TCCAGACTGC 
GATGAGAAGT TGTTTGGTGA CATCACTGAT AGGAGCTTTA GTCCTGACAG TGAGCCAGAC 
CTGGGAGGAT ACCTGGAAGA CAGCAGTCCT CAAGAAAGCC AAGATGAACT TGACCAGTAT 
ATCAGCCACC TTCTTCAAAG CACATCCTTG AAGTGTTACC TGCCCCCTCT TTGTAAGAAG 
TCAGAAGACA GCCATTTTTT CAGTGCTATT CACTCCACTC GGGACATACT TTCTGTGATG 
GCCAAAGCGA AGGGTTTGGA AACTACATTT TTTCCCATGA TTTTGCCAAC TAAAGTTCTA 
AGTGGACACC GTAAGTCACT GAATCTTGTT GACTCCCCTC AGCCACTCCT AAAGACGACT 
CCTGAATATG ACTACCAGTG GCCCAGAGAC GACCATGATG AAGTGGACTG TGAGAAGCTA 
GTTGGGCAAC TGAAAGACTG CTCAAACCTA CAGGACCAAG CAGACATTCT GTACATTCTT 
TATGTAATGA AGGGTCCCCG CTGGGATACC AATTTGTTTG GGCAGCATGG AGTCACTGTT 
CACAGTCTTC TCAGCGAGCT CTATGGAAAA GCTGGCCTAA ACCAAGAATG GAGTTTGATT 
CGCTACATTT CAGGCCTGCT CAGGAAGAAA GTGGAGGTCC TGGCTGAGGC CTGTGCAGAT 
CTGCTGTCCC ACCAGAAGCA GCTTACAGTA GGACTGCCCC CTGAGCCCCG GGAGAAGACC 
ATATCTACGC CTCTACCCCC AGAGGAGCTC ACAGAACTCA TATATGAAGC CAGTGGACAG 
GACATCAGCA TTGCTGTCCT CACACAGGAG ATCGTGGTTT ACCTGGCCAT GTATGTCCGG 
GCCCAGCCTA GCCTCTTTGC AGAGATGCTC AGACTCCGGA TTGGATTGAT CATCCAGGTG 
ATGGCCACAG AGCTGGCTCG GAGCCTGAAC TGCTCAGGAA AAGAAGCTTC GGAGAGCTTG 
ATGAACCTCA GCCCTTTCGA CATGAAGAGC CTTCTGCATC ATATCCTGAG TGGGAAGGAG 
TTCGGTGTGG AGAGAAGCGT GCGCCCAATA CACTCCTCCA TGTCCAGCCC TGCCATCTCC 
ATCCATGAGG TGGGTCATAC TGGAGCCACC AAAACTGAGC GGAGTGGCAT CACCAGACTG 
AGGAGTGAGA TGAAACAGAT GAATAGGCGA GCTAGTGCTG ATGAGCAGTT CTTTCCTTTG 
GGCCAGACCA TGTCCAACAG TTTGCATTCC ATCAAGTCTG TGAGGTCCAG CACCCCATCC 
TCCCCGACAG GCACATCATC TACAGACTCT GGAGGACAGC ACCTGGGCTG GGGAGAACAG 
CAGGGCCAGT GGCTGCGCCG GAGAAGGTTG GATGGGGCCA TTAACAGGGT CCCCGTGGGA 
TTCTACCAGA AAGTGTGGAA GATCCTTCAG AAGTGCCATG GCCTGTCCAT CGATGGTTAC 
GTCCTCCCAT CCTCGACAAC CCAAGAGATG ACCCCTTGTG AGATCAAGTT TGCCGTCCAT 
GTGGAGTCAG TGCTCAACCG TGTGTCCCAG CCTGAGTATC GGCAGCTACT GGTAGAAGCC 
ATCATGGTAC TGACTCTGCT CTCAGATACC GAAATGGACA GTATCGGGGG CATCATCCAT 
GTGGACCAGA TAGTGCAGCT GGCCAATCAG CTGTTCCTGC AGGACCAGGT GTCATTTGGA 
ACCACAGACA TCCTGGAAAA AGACCAAGCC ACAGGAATTT GCCACCTCTT TTATGACAGT 
GCTCCTAGTG GGGCTTATGG TACAATGACC TACCTAACAA AAGCGGTCGC TTCTCATTTG 
CAGGAACTGC TGCCCAGTTC AGGCTGCCAG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_006528679.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528742.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56570
Clone ID Related Accession (Same CDS sequence)	XM_006528679.4 , XM_006528679.3
Accession Version	XM_006528679.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG GCGAGATGCG GAGCAGGAGC AATTCTGGGG TCCGCCTGGA CGGGTACGCG 
AGGCTGGTGC AGCAAACCAT CCTGTGTTAC CAGAACCCAG TCACTGGGCT GTTATCAGCC 
AGCCATGACC AGAAGGATGC CTGGGTACGG GACAATATCT ATAGCATATT GGCTGTTTGG 
GGCCTGGGCA TGGCCTACCG CAAGAATGCT GACCGTGATG AGGATAAAGC CAAGGCCTAT 
GAGTTGGAAC AGAACGTAGT GAAGCTGATG CGAGGTCTTC TCCAGTGTAT GATGAGGCAG 
GTGGACAAAG TGGAGAAGTT CAAGCACACT CAGAGTACCA AGGACAGTCT ACATGCCAAG 
TACAACACTG CCACATGTAG CACTGTGGTG GGTGATGACC AGTGGGGCCA CCTCCAGGTG 
GATGCCACCT CTCTCTTCCT CCTGTTCCTG GCCCAGATGA CAGCCTCAGG TTTGCGTATT 
ATTTTTACCC TTGACGAAGT GGCCTTCATA CAGAATCTTG TCTTCTACAT AGAAGCTGCA 
TATAAAGTTG CTGATTATGG GATGTGGGAG CGTGGAGATA AAACCAATCA GGGCATTCCG 
GAACTGAATG CAAGCTCGGT GGGAGTGGCC AAGGCAGCAC TTGAGGCCAT TGATGAACTA 
GATCTTTTTG GAGCCCATGG AGGACGCAAA TCGGTGATCC ACGTCCTACC TGATGAAGTT 
GAGCACTGCC AGTCAATTCT TTTCTCCATG TTGCCAAGAG CATCAACATC TAAAGAAATC 
GATGCTGGAC TTCTTTCTAT TATTTCTTTC CCGGCCTTTG CAGTAGAAGA TGTGAACCTT 
GTGAATGTGA CCAAAAATGA AATTATTTCC AAGCTTCAGG GGCGGTATGG ATGCTGTCGC 
TTCCTTCGAG ATGGCTATAA AACCCCAAGA GAGGATCCAC ACCGATTGCA TTATGACCCT 
GCCGAACTTA AGCTCTTTGA AAACATTGAA TGTGAATGGC CTGTGTTCTG GACTTATTTA 
ATCATAGATG GAATCTTCAA TGGTGATGCT GTTCAGGTCC AAGAATACCG AGAAGCCTTG 
GAGGGAATAT TAATCAGAGG CAAAGATGGG ATCCACTTGG TGCCAGAACT CTATGCCATC 
CCACCAGACA AGGTGGATGA AGAGTATAAG AATCCACACA CAGTAGACCG AGTTCCACTG 
GGAAAGCTGC CCCATCTTTG GGGACAGTCC TTGTACATCC TCAGCTCACT GCTAGCAGAG 
GGATTCCTTG CCACGGGTGA AATCGATCCC TTAAATAGAA GATTTTCTAC TTCAGTCAAA 
CCTGATGTTG TAGTGCAAGT TGCTGTTTTG GCAGAAAACA GTCACATTAA GGGGCTGTTG 
AAGGAGCATG GAATGACTGT CCAGAGCATT GCTGATGTGC ATCCGATTCG AGTCCAGCCA 
GGCCGAATTC TTAGTCACAT ATATGCCAAA CTTGGACGAA ATAAGAATAT GAAATTGAGT 
GGTCGACCGT ATCGGCACAT TGGTGTCCTT GGCACCTCTA AACTCTATGT GATTAGGAAT 
CACATCTTCA CTTTTACACC CCAGTTCACT GACCAGCATC ACTTCTACCT GGCCCTGGAC 
AATGAGATGA TTGTGGAGAT GCTGAGGATC GAACTTGCCT ATCTGTGCAC CTGCTGGCGG 
ATGACCGGCC GACCCACTCT CACCTTCCCT GTCACGCATA CCATGCTCAC AAATGATGGA 
TCAGACATTC ATCCTGCAGT TCTTTCTACA ATTAGAAAAC TAGAAGATGG CTATTTTGGA 
GGTGCTAGAG TAAAACTAGG AAATCTGGCA GAGTTCCTTA CTACCTCATT CTACACACAC 
CTGACCTTCC TGGATCCAGA CTGCGATGAG AAGTTGTTTG GTGACATCAC TGATAGGAGC 
TTTAGTCCTG ACAGTGAGCC AGACCTGGGA GGATACCTGG AAGACAGCAG TCCTCAAGAA 
AGCCAAGATG AACTTGACCA GTATATCAGC CACCTTCTTC AAAGCACATC CTTGAAGTGT 
TACCTGCCCC CTCTTTGTAA GAAGTCAGAA GACAGCCATT TTTTCAGTGC TATTCACTCC 
ACTCGGGACA TACTTTCTGT GATGGCCAAA GCGAAGGGTT TGGAAACTAC ATTTTTTCCC 
ATGATTTTGC CAACTAAAGT TCTAAGTGGA CACCGTAAGT CACTGAATCT TGTTGACTCC 
CCTCAGCCAC TCCTAAAGAC GACTCCTGAA TATGACTACC AGTGGCCCAG AGACGACCAT 
GATGAAGTGG ACTGTGAGAA GCTAGTTGGG CAACTGAAAG ACTGCTCAAA CCTACAGGAC 
CAAGCAGACA TTCTGTACAT TCTTTATGTA ATGAAGGGTC CCCGCTGGGA TACCAATTTG 
TTTGGGCAGC ATGGAGTCAC TGTTCACAGT CTTCTCAGCG AGCTCTATGG AAAAGCTGGC 
CTAAACCAAG AATGGAGTTT GATTCGCTAC ATTTCAGGCC TGCTCAGGAA GAAAGTGGAG 
GTCCTGGCTG AGGCCTGTGC AGATCTGCTG TCCCACCAGA AGCAGCTTAC AGTAGGACTG 
CCCCCTGAGC CCCGGGAGAA GACCATATCT ACGCCTCTAC CCCCAGAGGA GCTCACAGAA 
CTCATATATG AAGCCAGTGG ACAGGACATC AGCATTGCTG TCCTCACACA GGAGATCGTG 
GTTTACCTGG CCATGTATGT CCGGGCCCAG CCTAGCCTCT TTGCAGAGAT GCTCAGACTC 
CGGATTGGAT TGATCATCCA GGTGATGGCC ACAGAGCTGG CTCGGAGCCT GAACTGCTCA 
GGAAAAGAAG CTTCGGAGAG CTTGATGAAC CTCAGCCCTT TCGACATGAA GAGCCTTCTG 
CATCATATCC TGAGTGGGAA GGAGTTCGGT GTGGAGAGAA GCGTGCGCCC AATACACTCC 
TCCATGTCCA GCCCTGCCAT CTCCATCCAT GAGGTGGGTC ATACTGGAGC CACCAAAACT 
GAGCGGAGTG GCATCACCAG ACTGAGGAGT GAGATGAAAC AGATGAATAG GCGAGCTAGT 
GCTGATGAGC AGTTCTTTCC TTTGGGCCAG ACCATGTCCA ACAGTTTGCA TTCCATCAAG 
TCTGTGAGGT CCAGCACCCC ATCCTCCCCG ACAGGCACAT CATCTACAGA CTCTGGAGGA 
CAGCACCTGG GCTGGGGAGA ACAGCAGGGC CAGTGGCTGC GCCGGAGAAG GTTGGATGGG 
GCCATTAACA GGGTCCCCGT GGGATTCTAC CAGAAAGTGT GGAAGATCCT TCAGAAGTGC 
CATGGCCTGT CCATCGATGG TTACGTCCTC CCATCCTCGA CAACCCAAGA GATGACCCCT 
TGTGAGATCA AGTTTGCCGT CCATGTGGAG TCAGTGCTCA ACCGTGTGTC CCAGCCTGAG 
TATCGGCAGC TACTGGTAGA AGCCATCATG GTACTGACTC TGCTCTCAGA TACCGAAATG 
GACAGTATCG GGGGCATCAT CCATGTGGAC CAGATAGTGC AGCTGGCCAA TCAGCTGTTC 
CTGCAGGACC AGGTGTCATT TGGAACCACA GACATCCTGG AAAAAGACCA AGCCACAGGA 
ATTTGCCACC TCTTTTATGA CAGTGCTCCT AGTGGGGCTT ATGGTACAAT GACCTACCTA 
ACAAAAGCGG TCGCTTCTCA TTTGCAGGAA CTGCTGCCCA GTTCAGGCTG CCAGATGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528742.1
CDS	198..3920
Translation	MSLGEMRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	XM_006528679.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528679.3

ORF Insert Sequence:

ATGAGCTTGG GCGAGATGCG GAGCAGGAGC AATTCTGGGG TCCGCCTGGA CGGGTACGCG 
AGGCTGGTGC AGCAAACCAT CCTGTGTTAC CAGAACCCAG TCACTGGGCT GTTATCAGCC 
AGCCATGACC AGAAGGATGC CTGGGTACGG GACAATATCT ATAGCATATT GGCTGTTTGG 
GGCCTGGGCA TGGCCTACCG CAAGAATGCT GACCGTGATG AGGATAAAGC CAAGGCCTAT 
GAGTTGGAAC AGAACGTAGT GAAGCTGATG CGAGGTCTTC TCCAGTGTAT GATGAGGCAG 
GTGGACAAAG TGGAGAAGTT CAAGCACACT CAGAGTACCA AGGACAGTCT ACATGCCAAG 
TACAACACTG CCACATGTAG CACTGTGGTG GGTGATGACC AGTGGGGCCA CCTCCAGGTG 
GATGCCACCT CTCTCTTCCT CCTGTTCCTG GCCCAGATGA CAGCCTCAGG TTTGCGTATT 
ATTTTTACCC TTGACGAAGT GGCCTTCATA CAGAATCTTG TCTTCTACAT AGAAGCTGCA 
TATAAAGTTG CTGATTATGG GATGTGGGAG CGTGGAGATA AAACCAATCA GGGCATTCCG 
GAACTGAATG CAAGCTCGGT GGGAGTGGCC AAGGCAGCAC TTGAGGCCAT TGATGAACTA 
GATCTTTTTG GAGCCCATGG AGGACGCAAA TCGGTGATCC ACGTCCTACC TGATGAAGTT 
GAGCACTGCC AGTCAATTCT TTTCTCCATG TTGCCAAGAG CATCAACATC TAAAGAAATC 
GATGCTGGAC TTCTTTCTAT TATTTCTTTC CCGGCCTTTG CAGTAGAAGA TGTGAACCTT 
GTGAATGTGA CCAAAAATGA AATTATTTCC AAGCTTCAGG GGCGGTATGG ATGCTGTCGC 
TTCCTTCGAG ATGGCTATAA AACCCCAAGA GAGGATCCAC ACCGATTGCA TTATGACCCT 
GCCGAACTTA AGCTCTTTGA AAACATTGAA TGTGAATGGC CTGTGTTCTG GACTTATTTA 
ATCATAGATG GAATCTTCAA TGGTGATGCT GTTCAGGTCC AAGAATACCG AGAAGCCTTG 
GAGGGAATAT TAATCAGAGG CAAAGATGGG ATCCACTTGG TGCCAGAACT CTATGCCATC 
CCACCAGACA AGGTGGATGA AGAGTATAAG AATCCACACA CAGTAGACCG AGTTCCACTG 
GGAAAGCTGC CCCATCTTTG GGGACAGTCC TTGTACATCC TCAGCTCACT GCTAGCAGAG 
GGATTCCTTG CCACGGGTGA AATCGATCCC TTAAATAGAA GATTTTCTAC TTCAGTCAAA 
CCTGATGTTG TAGTGCAAGT TGCTGTTTTG GCAGAAAACA GTCACATTAA GGGGCTGTTG 
AAGGAGCATG GAATGACTGT CCAGAGCATT GCTGATGTGC ATCCGATTCG AGTCCAGCCA 
GGCCGAATTC TTAGTCACAT ATATGCCAAA CTTGGACGAA ATAAGAATAT GAAATTGAGT 
GGTCGACCGT ATCGGCACAT TGGTGTCCTT GGCACCTCTA AACTCTATGT GATTAGGAAT 
CACATCTTCA CTTTTACACC CCAGTTCACT GACCAGCATC ACTTCTACCT GGCCCTGGAC 
AATGAGATGA TTGTGGAGAT GCTGAGGATC GAACTTGCCT ATCTGTGCAC CTGCTGGCGG 
ATGACCGGCC GACCCACTCT CACCTTCCCT GTCACGCATA CCATGCTCAC AAATGATGGA 
TCAGACATTC ATCCTGCAGT TCTTTCTACA ATTAGAAAAC TAGAAGATGG CTATTTTGGA 
GGTGCTAGAG TAAAACTAGG AAATCTGGCA GAGTTCCTTA CTACCTCATT CTACACACAC 
CTGACCTTCC TGGATCCAGA CTGCGATGAG AAGTTGTTTG GTGACATCAC TGATAGGAGC 
TTTAGTCCTG ACAGTGAGCC AGACCTGGGA GGATACCTGG AAGACAGCAG TCCTCAAGAA 
AGCCAAGATG AACTTGACCA GTATATCAGC CACCTTCTTC AAAGCACATC CTTGAAGTGT 
TACCTGCCCC CTCTTTGTAA GAAGTCAGAA GACAGCCATT TTTTCAGTGC TATTCACTCC 
ACTCGGGACA TACTTTCTGT GATGGCCAAA GCGAAGGGTT TGGAAACTAC ATTTTTTCCC 
ATGATTTTGC CAACTAAAGT TCTAAGTGGA CACCGTAAGT CACTGAATCT TGTTGACTCC 
CCTCAGCCAC TCCTAAAGAC GACTCCTGAA TATGACTACC AGTGGCCCAG AGACGACCAT 
GATGAAGTGG ACTGTGAGAA GCTAGTTGGG CAACTGAAAG ACTGCTCAAA CCTACAGGAC 
CAAGCAGACA TTCTGTACAT TCTTTATGTA ATGAAGGGTC CCCGCTGGGA TACCAATTTG 
TTTGGGCAGC ATGGAGTCAC TGTTCACAGT CTTCTCAGCG AGCTCTATGG AAAAGCTGGC 
CTAAACCAAG AATGGAGTTT GATTCGCTAC ATTTCAGGCC TGCTCAGGAA GAAAGTGGAG 
GTCCTGGCTG AGGCCTGTGC AGATCTGCTG TCCCACCAGA AGCAGCTTAC AGTAGGACTG 
CCCCCTGAGC CCCGGGAGAA GACCATATCT ACGCCTCTAC CCCCAGAGGA GCTCACAGAA 
CTCATATATG AAGCCAGTGG ACAGGACATC AGCATTGCTG TCCTCACACA GGAGATCGTG 
GTTTACCTGG CCATGTATGT CCGGGCCCAG CCTAGCCTCT TTGCAGAGAT GCTCAGACTC 
CGGATTGGAT TGATCATCCA GGTGATGGCC ACAGAGCTGG CTCGGAGCCT GAACTGCTCA 
GGAAAAGAAG CTTCGGAGAG CTTGATGAAC CTCAGCCCTT TCGACATGAA GAGCCTTCTG 
CATCATATCC TGAGTGGGAA GGAGTTCGGT GTGGAGAGAA GCGTGCGCCC AATACACTCC 
TCCATGTCCA GCCCTGCCAT CTCCATCCAT GAGGTGGGTC ATACTGGAGC CACCAAAACT 
GAGCGGAGTG GCATCACCAG ACTGAGGAGT GAGATGAAAC AGATGAATAG GCGAGCTAGT 
GCTGATGAGC AGTTCTTTCC TTTGGGCCAG ACCATGTCCA ACAGTTTGCA TTCCATCAAG 
TCTGTGAGGT CCAGCACCCC ATCCTCCCCG ACAGGCACAT CATCTACAGA CTCTGGAGGA 
CAGCACCTGG GCTGGGGAGA ACAGCAGGGC CAGTGGCTGC GCCGGAGAAG GTTGGATGGG 
GCCATTAACA GGGTCCCCGT GGGATTCTAC CAGAAAGTGT GGAAGATCCT TCAGAAGTGC 
CATGGCCTGT CCATCGATGG TTACGTCCTC CCATCCTCGA CAACCCAAGA GATGACCCCT 
TGTGAGATCA AGTTTGCCGT CCATGTGGAG TCAGTGCTCA ACCGTGTGTC CCAGCCTGAG 
TATCGGCAGC TACTGGTAGA AGCCATCATG GTACTGACTC TGCTCTCAGA TACCGAAATG 
GACAGTATCG GGGGCATCAT CCATGTGGAC CAGATAGTGC AGCTGGCCAA TCAGCTGTTC 
CTGCAGGACC AGGTGTCATT TGGAACCACA GACATCCTGG AAAAAGACCA AGCCACAGGA 
ATTTGCCACC TCTTTTATGA CAGTGCTCCT AGTGGGGCTT ATGGTACAAT GACCTACCTA 
ACAAAAGCGG TCGCTTCTCA TTTGCAGGAA CTGCTGCCCA GTTCAGGCTG CCAGATGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_006528681.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528744.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu56572
Clone ID Related Accession (Same CDS sequence)	XM_006528681.1
Accession Version	XM_006528681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTACC GCAAGAATGC TGACCGTGAT GAGGATAAAG CCAAGGCCTA TGAGTTGGAA 
CAGAACGTAG TGAAGCTGAT GCGAGGTCTT CTCCAGTGTA TGATGAGGCA GGTGGACAAA 
GTGGAGAAGT TCAAGCACAC TCAGAGTACC AAGGACAGTC TACATGCCAA GTACAACACT 
GCCACATGTA GCACTGTGGT GGGTGATGAC CAGTGGGGCC ACCTCCAGGT GGATGCCACC 
TCTCTCTTCC TCCTGTTCCT GGCCCAGATG ACAGCCTCAG GTTTGCGTAT TATTTTTACC 
CTTGACGAAG TGGCCTTCAT ACAGAATCTT GTCTTCTACA TAGAAGCTGC ATATAAAGTT 
GCTGATTATG GGATGTGGGA GCGTGGAGAT AAAACCAATC AGGGCATTCC GGAACTGAAT 
GCAAGCTCGG TGGGAGTGGC CAAGGCAGCA CTTGAGGCCA TTGATGAACT AGATCTTTTT 
GGAGCCCATG GAGGACGCAA ATCGGTGATC CACGTCCTAC CTGATGAAGT TGAGCACTGC 
CAGTCAATTC TTTTCTCCAT GTTGCCAAGA GCATCAACAT CTAAAGAAAT CGATGCTGGA 
CTTCTTTCTA TTATTTCTTT CCCGGCCTTT GCAGTAGAAG ATGTGAACCT TGTGAATGTG 
ACCAAAAATG AAATTATTTC CAAGCTTCAG GGGCGGTATG GATGCTGTCG CTTCCTTCGA 
GATGGCTATA AAACCCCAAG AGAGGATCCA CACCGATTGC ATTATGACCC TGCCGAACTT 
AAGCTCTTTG AAAACATTGA ATGTGAATGG CCTGTGTTCT GGACTTATTT AATCATAGAT 
GGAATCTTCA ATGGTGATGC TGTTCAGGTC CAAGAATACC GAGAAGCCTT GGAGGGAATA 
TTAATCAGAG GCAAAGATGG GATCCACTTG GTGCCAGAAC TCTATGCCAT CCCACCAGAC 
AAGGTGGATG AAGAGTATAA GAATCCACAC ACAGTAGACC GAGTTCCACT GGGAAAGCTG 
CCCCATCTTT GGGGACAGTC CTTGTACATC CTCAGCTCAC TGCTAGCAGA GGGATTCCTT 
GCCACGGGTG AAATCGATCC CTTAAATAGA AGATTTTCTA CTTCAGTCAA ACCTGATGTT 
GTAGTGCAAG TTGCTGTTTT GGCAGAAAAC AGTCACATTA AGGGGCTGTT GAAGGAGCAT 
GGAATGACTG TCCAGAGCAT TGCTGATGTG CATCCGATTC GAGTCCAGCC AGGCCGAATT 
CTTAGTCACA TATATGCCAA ACTTGGACGA AATAAGAATA TGAAATTGAG TGGTCGACCG 
TATCGGCACA TTGGTGTCCT TGGCACCTCT AAACTCTATG TGATTAGGAA TCACATCTTC 
ACTTTTACAC CCCAGTTCAC TGACCAGCAT CACTTCTACC TGGCCCTGGA CAATGAGATG 
ATTGTGGAGA TGCTGAGGAT CGAACTTGCC TATCTGTGCA CCTGCTGGCG GATGACCGGC 
CGACCCACTC TCACCTTCCC TGTCACGCAT ACCATGCTCA CAAATGATGG ATCAGACATT 
CATCCTGCAG TTCTTTCTAC AATTAGAAAA CTAGAAGATG GCTATTTTGG AGGTGCTAGA 
GTAAAACTAG GAAATCTGGC AGAGTTCCTT ACTACCTCAT TCTACACACA CCTGACCTTC 
CTGGATCCAG ACTGCGATGA GAAGTTGTTT GGTGACATCA CTGATAGGAG CTTTAGTCCT 
GACAGTGAGC CAGACCTGGG AGGATACCTG GAAGACAGCA GTCCTCAAGA AAGCCAAGAT 
GAACTTGACC AGTATATCAG CCACCTTCTT CAAAGCACAT CCTTGAAGTG TTACCTGCCC 
CCTCTTTGTA AGAAGTCAGA AGACAGCCAT TTTTTCAGTG CTATTCACTC CACTCGGGAC 
ATACTTTCTG TGATGGCCAA AGCGAAGGGT TTGGAAACTA CATTTTTTCC CATGATTTTG 
CCAACTAAAG TTCTAAGTGG ACACCGTAAG TCACTGAATC TTGTTGACTC CCCTCAGCCA 
CTCCTAAAGA CGACTCCTGA ATATGACTAC CAGTGGCCCA GAGACGACCA TGATGAAGTG 
GACTGTGAGA AGCTAGTTGG GCAACTGAAA GACTGCTCAA ACCTACAGGA CCAAGCAGAC 
ATTCTGTACA TTCTTTATGT AATGAAGGGT CCCCGCTGGG ATACCAATTT GTTTGGGCAG 
CATGGAGTCA CTGTTCACAG TCTTCTCAGC GAGCTCTATG GAAAAGCTGG CCTAAACCAA 
GAATGGAGTT TGATTCGCTA CATTTCAGGC CTGCTCAGGA AGAAAGTGGA GGTCCTGGCT 
GAGGCCTGTG CAGATCTGCT GTCCCACCAG AAGCAGCTTA CAGTAGGACT GCCCCCTGAG 
CCCCGGGAGA AGACCATATC TACGCCTCTA CCCCCAGAGG AGCTCACAGA ACTCATATAT 
GAAGCCAGTG GACAGGACAT CAGCATTGCT GTCCTCACAC AGGAGATCGT GGTTTACCTG 
GCCATGTATG TCCGGGCCCA GCCTAGCCTC TTTGCAGAGA TGCTCAGACT CCGGATTGGA 
TTGATCATCC AGGTGATGGC CACAGAGCTG GCTCGGAGCC TGAACTGCTC AGGAAAAGAA 
GCTTCGGAGA GCTTGATGAA CCTCAGCCCT TTCGACATGA AGAGCCTTCT GCATCATATC 
CTGAGTGGGA AGGAGTTCGG TGTGGAGAGA AGCGTGCGCC CAATACACTC CTCCATGTCC 
AGCCCTGCCA TCTCCATCCA TGAGGTGGGT CATACTGGAG CCACCAAAAC TGAGCGGAGT 
GGCATCACCA GACTGAGGAG TGAGATGAAA CAGATGAATA GGCGAGCTAG TGCTGATGAG 
CAGTTCTTTC CTTTGGGCCA GACCATGTCC AACAGTTTGC ATTCCATCAA GTCTGTGAGG 
TCCAGCACCC CATCCTCCCC GACAGGCACA TCATCTACAG ACTCTGGAGG ACAGCACCTG 
GGCTGGGGAG AACAGCAGGG CCAGTGGCTG CGCCGGAGAA GGTTGGATGG GGCCATTAAC 
AGGGTCCCCG TGGGATTCTA CCAGAAAGTG TGGAAGATCC TTCAGAAGTG CCATGGCCTG 
TCCATCGATG GTTACGTCCT CCCATCCTCG ACAACCCAAG AGATGACCCC TTGTGAGATC 
AAGTTTGCCG TCCATGTGGA GTCAGTGCTC AACCGTGTGT CCCAGCCTGA GTATCGGCAG 
CTACTGGTAG AAGCCATCAT GGTACTGACT CTGCTCTCAG ATACCGAAAT GGACAGTATC 
GGGGGCATCA TCCATGTGGA CCAGATAGTG CAGCTGGCCA ATCAGCTGTT CCTGCAGGAC 
CAGGTGTCAT TTGGAACCAC AGACATCCTG GAAAAAGACC AAGCCACAGG AATTTGCCAC 
CTCTTTTATG ACAGTGCTCC TAGTGGGGCT TATGGTACAA TGACCTACCT AACAAAAGCG 
GTCGCTTCTC ATTTGCAGGA ACTGCTGCCC AGTTCAGGCT GCCAGATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528744.1
CDS	116..3649
Translation	MAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	XM_006528681.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528681.1

ORF Insert Sequence:

ATGGCCTACC GCAAGAATGC TGACCGTGAT GAGGATAAAG CCAAGGCCTA TGAGTTGGAA 
CAGAACGTAG TGAAGCTGAT GCGAGGTCTT CTCCAGTGTA TGATGAGGCA GGTGGACAAA 
GTGGAGAAGT TCAAGCACAC TCAGAGTACC AAGGACAGTC TACATGCCAA GTACAACACT 
GCCACATGTA GCACTGTGGT GGGTGATGAC CAGTGGGGCC ACCTCCAGGT GGATGCCACC 
TCTCTCTTCC TCCTGTTCCT GGCCCAGATG ACAGCCTCAG GTTTGCGTAT TATTTTTACC 
CTTGACGAAG TGGCCTTCAT ACAGAATCTT GTCTTCTACA TAGAAGCTGC ATATAAAGTT 
GCTGATTATG GGATGTGGGA GCGTGGAGAT AAAACCAATC AGGGCATTCC GGAACTGAAT 
GCAAGCTCGG TGGGAGTGGC CAAGGCAGCA CTTGAGGCCA TTGATGAACT AGATCTTTTT 
GGAGCCCATG GAGGACGCAA ATCGGTGATC CACGTCCTAC CTGATGAAGT TGAGCACTGC 
CAGTCAATTC TTTTCTCCAT GTTGCCAAGA GCATCAACAT CTAAAGAAAT CGATGCTGGA 
CTTCTTTCTA TTATTTCTTT CCCGGCCTTT GCAGTAGAAG ATGTGAACCT TGTGAATGTG 
ACCAAAAATG AAATTATTTC CAAGCTTCAG GGGCGGTATG GATGCTGTCG CTTCCTTCGA 
GATGGCTATA AAACCCCAAG AGAGGATCCA CACCGATTGC ATTATGACCC TGCCGAACTT 
AAGCTCTTTG AAAACATTGA ATGTGAATGG CCTGTGTTCT GGACTTATTT AATCATAGAT 
GGAATCTTCA ATGGTGATGC TGTTCAGGTC CAAGAATACC GAGAAGCCTT GGAGGGAATA 
TTAATCAGAG GCAAAGATGG GATCCACTTG GTGCCAGAAC TCTATGCCAT CCCACCAGAC 
AAGGTGGATG AAGAGTATAA GAATCCACAC ACAGTAGACC GAGTTCCACT GGGAAAGCTG 
CCCCATCTTT GGGGACAGTC CTTGTACATC CTCAGCTCAC TGCTAGCAGA GGGATTCCTT 
GCCACGGGTG AAATCGATCC CTTAAATAGA AGATTTTCTA CTTCAGTCAA ACCTGATGTT 
GTAGTGCAAG TTGCTGTTTT GGCAGAAAAC AGTCACATTA AGGGGCTGTT GAAGGAGCAT 
GGAATGACTG TCCAGAGCAT TGCTGATGTG CATCCGATTC GAGTCCAGCC AGGCCGAATT 
CTTAGTCACA TATATGCCAA ACTTGGACGA AATAAGAATA TGAAATTGAG TGGTCGACCG 
TATCGGCACA TTGGTGTCCT TGGCACCTCT AAACTCTATG TGATTAGGAA TCACATCTTC 
ACTTTTACAC CCCAGTTCAC TGACCAGCAT CACTTCTACC TGGCCCTGGA CAATGAGATG 
ATTGTGGAGA TGCTGAGGAT CGAACTTGCC TATCTGTGCA CCTGCTGGCG GATGACCGGC 
CGACCCACTC TCACCTTCCC TGTCACGCAT ACCATGCTCA CAAATGATGG ATCAGACATT 
CATCCTGCAG TTCTTTCTAC AATTAGAAAA CTAGAAGATG GCTATTTTGG AGGTGCTAGA 
GTAAAACTAG GAAATCTGGC AGAGTTCCTT ACTACCTCAT TCTACACACA CCTGACCTTC 
CTGGATCCAG ACTGCGATGA GAAGTTGTTT GGTGACATCA CTGATAGGAG CTTTAGTCCT 
GACAGTGAGC CAGACCTGGG AGGATACCTG GAAGACAGCA GTCCTCAAGA AAGCCAAGAT 
GAACTTGACC AGTATATCAG CCACCTTCTT CAAAGCACAT CCTTGAAGTG TTACCTGCCC 
CCTCTTTGTA AGAAGTCAGA AGACAGCCAT TTTTTCAGTG CTATTCACTC CACTCGGGAC 
ATACTTTCTG TGATGGCCAA AGCGAAGGGT TTGGAAACTA CATTTTTTCC CATGATTTTG 
CCAACTAAAG TTCTAAGTGG ACACCGTAAG TCACTGAATC TTGTTGACTC CCCTCAGCCA 
CTCCTAAAGA CGACTCCTGA ATATGACTAC CAGTGGCCCA GAGACGACCA TGATGAAGTG 
GACTGTGAGA AGCTAGTTGG GCAACTGAAA GACTGCTCAA ACCTACAGGA CCAAGCAGAC 
ATTCTGTACA TTCTTTATGT AATGAAGGGT CCCCGCTGGG ATACCAATTT GTTTGGGCAG 
CATGGAGTCA CTGTTCACAG TCTTCTCAGC GAGCTCTATG GAAAAGCTGG CCTAAACCAA 
GAATGGAGTT TGATTCGCTA CATTTCAGGC CTGCTCAGGA AGAAAGTGGA GGTCCTGGCT 
GAGGCCTGTG CAGATCTGCT GTCCCACCAG AAGCAGCTTA CAGTAGGACT GCCCCCTGAG 
CCCCGGGAGA AGACCATATC TACGCCTCTA CCCCCAGAGG AGCTCACAGA ACTCATATAT 
GAAGCCAGTG GACAGGACAT CAGCATTGCT GTCCTCACAC AGGAGATCGT GGTTTACCTG 
GCCATGTATG TCCGGGCCCA GCCTAGCCTC TTTGCAGAGA TGCTCAGACT CCGGATTGGA 
TTGATCATCC AGGTGATGGC CACAGAGCTG GCTCGGAGCC TGAACTGCTC AGGAAAAGAA 
GCTTCGGAGA GCTTGATGAA CCTCAGCCCT TTCGACATGA AGAGCCTTCT GCATCATATC 
CTGAGTGGGA AGGAGTTCGG TGTGGAGAGA AGCGTGCGCC CAATACACTC CTCCATGTCC 
AGCCCTGCCA TCTCCATCCA TGAGGTGGGT CATACTGGAG CCACCAAAAC TGAGCGGAGT 
GGCATCACCA GACTGAGGAG TGAGATGAAA CAGATGAATA GGCGAGCTAG TGCTGATGAG 
CAGTTCTTTC CTTTGGGCCA GACCATGTCC AACAGTTTGC ATTCCATCAA GTCTGTGAGG 
TCCAGCACCC CATCCTCCCC GACAGGCACA TCATCTACAG ACTCTGGAGG ACAGCACCTG 
GGCTGGGGAG AACAGCAGGG CCAGTGGCTG CGCCGGAGAA GGTTGGATGG GGCCATTAAC 
AGGGTCCCCG TGGGATTCTA CCAGAAAGTG TGGAAGATCC TTCAGAAGTG CCATGGCCTG 
TCCATCGATG GTTACGTCCT CCCATCCTCG ACAACCCAAG AGATGACCCC TTGTGAGATC 
AAGTTTGCCG TCCATGTGGA GTCAGTGCTC AACCGTGTGT CCCAGCCTGA GTATCGGCAG 
CTACTGGTAG AAGCCATCAT GGTACTGACT CTGCTCTCAG ATACCGAAAT GGACAGTATC 
GGGGGCATCA TCCATGTGGA CCAGATAGTG CAGCTGGCCA ATCAGCTGTT CCTGCAGGAC 
CAGGTGTCAT TTGGAACCAC AGACATCCTG GAAAAAGACC AAGCCACAGG AATTTGCCAC 
CTCTTTTATG ACAGTGCTCC TAGTGGGGCT TATGGTACAA TGACCTACCT AACAAAAGCG 
GTCGCTTCTC ATTTGCAGGA ACTGCTGCCC AGTTCAGGCT GCCAGATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_006528682.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528745.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X6, mRNA.

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CloneID	OMu56573
Clone ID Related Accession (Same CDS sequence)	XM_006528682.1 , XM_030251193.1 , XM_030251194.1
Accession Version	XM_006528682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGAGC GTGGAGATAA AACCAATCAG GGCATTCCGG AACTGAATGC AAGCTCGGTG 
GGAGTGGCCA AGGCAGCACT TGAGGCCATT GATGAACTAG ATCTTTTTGG AGCCCATGGA 
GGACGCAAAT CGGTGATCCA CGTCCTACCT GATGAAGTTG AGCACTGCCA GTCAATTCTT 
TTCTCCATGT TGCCAAGAGC ATCAACATCT AAAGAAATCG ATGCTGGACT TCTTTCTATT 
ATTTCTTTCC CGGCCTTTGC AGTAGAAGAT GTGAACCTTG TGAATGTGAC CAAAAATGAA 
ATTATTTCCA AGCTTCAGGG GCGGTATGGA TGCTGTCGCT TCCTTCGAGA TGGCTATAAA 
ACCCCAAGAG AGGATCCACA CCGATTGCAT TATGACCCTG CCGAACTTAA GCTCTTTGAA 
AACATTGAAT GTGAATGGCC TGTGTTCTGG ACTTATTTAA TCATAGATGG AATCTTCAAT 
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAAGCCTTGG AGGGAATATT AATCAGAGGC 
AAAGATGGGA TCCACTTGGT GCCAGAACTC TATGCCATCC CACCAGACAA GGTGGATGAA 
GAGTATAAGA ATCCACACAC AGTAGACCGA GTTCCACTGG GAAAGCTGCC CCATCTTTGG 
GGACAGTCCT TGTACATCCT CAGCTCACTG CTAGCAGAGG GATTCCTTGC CACGGGTGAA 
ATCGATCCCT TAAATAGAAG ATTTTCTACT TCAGTCAAAC CTGATGTTGT AGTGCAAGTT 
GCTGTTTTGG CAGAAAACAG TCACATTAAG GGGCTGTTGA AGGAGCATGG AATGACTGTC 
CAGAGCATTG CTGATGTGCA TCCGATTCGA GTCCAGCCAG GCCGAATTCT TAGTCACATA 
TATGCCAAAC TTGGACGAAA TAAGAATATG AAATTGAGTG GTCGACCGTA TCGGCACATT 
GGTGTCCTTG GCACCTCTAA ACTCTATGTG ATTAGGAATC ACATCTTCAC TTTTACACCC 
CAGTTCACTG ACCAGCATCA CTTCTACCTG GCCCTGGACA ATGAGATGAT TGTGGAGATG 
CTGAGGATCG AACTTGCCTA TCTGTGCACC TGCTGGCGGA TGACCGGCCG ACCCACTCTC 
ACCTTCCCTG TCACGCATAC CATGCTCACA AATGATGGAT CAGACATTCA TCCTGCAGTT 
CTTTCTACAA TTAGAAAACT AGAAGATGGC TATTTTGGAG GTGCTAGAGT AAAACTAGGA 
AATCTGGCAG AGTTCCTTAC TACCTCATTC TACACACACC TGACCTTCCT GGATCCAGAC 
TGCGATGAGA AGTTGTTTGG TGACATCACT GATAGGAGCT TTAGTCCTGA CAGTGAGCCA 
GACCTGGGAG GATACCTGGA AGACAGCAGT CCTCAAGAAA GCCAAGATGA ACTTGACCAG 
TATATCAGCC ACCTTCTTCA AAGCACATCC TTGAAGTGTT ACCTGCCCCC TCTTTGTAAG 
AAGTCAGAAG ACAGCCATTT TTTCAGTGCT ATTCACTCCA CTCGGGACAT ACTTTCTGTG 
ATGGCCAAAG CGAAGGGTTT GGAAACTACA TTTTTTCCCA TGATTTTGCC AACTAAAGTT 
CTAAGTGGAC ACCGTAAGTC ACTGAATCTT GTTGACTCCC CTCAGCCACT CCTAAAGACG 
ACTCCTGAAT ATGACTACCA GTGGCCCAGA GACGACCATG ATGAAGTGGA CTGTGAGAAG 
CTAGTTGGGC AACTGAAAGA CTGCTCAAAC CTACAGGACC AAGCAGACAT TCTGTACATT 
CTTTATGTAA TGAAGGGTCC CCGCTGGGAT ACCAATTTGT TTGGGCAGCA TGGAGTCACT 
GTTCACAGTC TTCTCAGCGA GCTCTATGGA AAAGCTGGCC TAAACCAAGA ATGGAGTTTG 
ATTCGCTACA TTTCAGGCCT GCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGTGCA 
GATCTGCTGT CCCACCAGAA GCAGCTTACA GTAGGACTGC CCCCTGAGCC CCGGGAGAAG 
ACCATATCTA CGCCTCTACC CCCAGAGGAG CTCACAGAAC TCATATATGA AGCCAGTGGA 
CAGGACATCA GCATTGCTGT CCTCACACAG GAGATCGTGG TTTACCTGGC CATGTATGTC 
CGGGCCCAGC CTAGCCTCTT TGCAGAGATG CTCAGACTCC GGATTGGATT GATCATCCAG 
GTGATGGCCA CAGAGCTGGC TCGGAGCCTG AACTGCTCAG GAAAAGAAGC TTCGGAGAGC 
TTGATGAACC TCAGCCCTTT CGACATGAAG AGCCTTCTGC ATCATATCCT GAGTGGGAAG 
GAGTTCGGTG TGGAGAGAAG CGTGCGCCCA ATACACTCCT CCATGTCCAG CCCTGCCATC 
TCCATCCATG AGGTGGGTCA TACTGGAGCC ACCAAAACTG AGCGGAGTGG CATCACCAGA 
CTGAGGAGTG AGATGAAACA GATGAATAGG CGAGCTAGTG CTGATGAGCA GTTCTTTCCT 
TTGGGCCAGA CCATGTCCAA CAGTTTGCAT TCCATCAAGT CTGTGAGGTC CAGCACCCCA 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC AGCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CCGGAGAAGG TTGGATGGGG CCATTAACAG GGTCCCCGTG 
GGATTCTACC AGAAAGTGTG GAAGATCCTT CAGAAGTGCC ATGGCCTGTC CATCGATGGT 
TACGTCCTCC CATCCTCGAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTTGCCGTC 
CATGTGGAGT CAGTGCTCAA CCGTGTGTCC CAGCCTGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACTCT GCTCTCAGAT ACCGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTGGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ACATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCTA GTGGGGCTTA TGGTACAATG ACCTACCTAA CAAAAGCGGT CGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAG TTCAGGCTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528745.1
CDS	294..3455
Translation	MWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	XM_006528682.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528682.1

ORF Insert Sequence:

ATGTGGGAGC GTGGAGATAA AACCAATCAG GGCATTCCGG AACTGAATGC AAGCTCGGTG 
GGAGTGGCCA AGGCAGCACT TGAGGCCATT GATGAACTAG ATCTTTTTGG AGCCCATGGA 
GGACGCAAAT CGGTGATCCA CGTCCTACCT GATGAAGTTG AGCACTGCCA GTCAATTCTT 
TTCTCCATGT TGCCAAGAGC ATCAACATCT AAAGAAATCG ATGCTGGACT TCTTTCTATT 
ATTTCTTTCC CGGCCTTTGC AGTAGAAGAT GTGAACCTTG TGAATGTGAC CAAAAATGAA 
ATTATTTCCA AGCTTCAGGG GCGGTATGGA TGCTGTCGCT TCCTTCGAGA TGGCTATAAA 
ACCCCAAGAG AGGATCCACA CCGATTGCAT TATGACCCTG CCGAACTTAA GCTCTTTGAA 
AACATTGAAT GTGAATGGCC TGTGTTCTGG ACTTATTTAA TCATAGATGG AATCTTCAAT 
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAAGCCTTGG AGGGAATATT AATCAGAGGC 
AAAGATGGGA TCCACTTGGT GCCAGAACTC TATGCCATCC CACCAGACAA GGTGGATGAA 
GAGTATAAGA ATCCACACAC AGTAGACCGA GTTCCACTGG GAAAGCTGCC CCATCTTTGG 
GGACAGTCCT TGTACATCCT CAGCTCACTG CTAGCAGAGG GATTCCTTGC CACGGGTGAA 
ATCGATCCCT TAAATAGAAG ATTTTCTACT TCAGTCAAAC CTGATGTTGT AGTGCAAGTT 
GCTGTTTTGG CAGAAAACAG TCACATTAAG GGGCTGTTGA AGGAGCATGG AATGACTGTC 
CAGAGCATTG CTGATGTGCA TCCGATTCGA GTCCAGCCAG GCCGAATTCT TAGTCACATA 
TATGCCAAAC TTGGACGAAA TAAGAATATG AAATTGAGTG GTCGACCGTA TCGGCACATT 
GGTGTCCTTG GCACCTCTAA ACTCTATGTG ATTAGGAATC ACATCTTCAC TTTTACACCC 
CAGTTCACTG ACCAGCATCA CTTCTACCTG GCCCTGGACA ATGAGATGAT TGTGGAGATG 
CTGAGGATCG AACTTGCCTA TCTGTGCACC TGCTGGCGGA TGACCGGCCG ACCCACTCTC 
ACCTTCCCTG TCACGCATAC CATGCTCACA AATGATGGAT CAGACATTCA TCCTGCAGTT 
CTTTCTACAA TTAGAAAACT AGAAGATGGC TATTTTGGAG GTGCTAGAGT AAAACTAGGA 
AATCTGGCAG AGTTCCTTAC TACCTCATTC TACACACACC TGACCTTCCT GGATCCAGAC 
TGCGATGAGA AGTTGTTTGG TGACATCACT GATAGGAGCT TTAGTCCTGA CAGTGAGCCA 
GACCTGGGAG GATACCTGGA AGACAGCAGT CCTCAAGAAA GCCAAGATGA ACTTGACCAG 
TATATCAGCC ACCTTCTTCA AAGCACATCC TTGAAGTGTT ACCTGCCCCC TCTTTGTAAG 
AAGTCAGAAG ACAGCCATTT TTTCAGTGCT ATTCACTCCA CTCGGGACAT ACTTTCTGTG 
ATGGCCAAAG CGAAGGGTTT GGAAACTACA TTTTTTCCCA TGATTTTGCC AACTAAAGTT 
CTAAGTGGAC ACCGTAAGTC ACTGAATCTT GTTGACTCCC CTCAGCCACT CCTAAAGACG 
ACTCCTGAAT ATGACTACCA GTGGCCCAGA GACGACCATG ATGAAGTGGA CTGTGAGAAG 
CTAGTTGGGC AACTGAAAGA CTGCTCAAAC CTACAGGACC AAGCAGACAT TCTGTACATT 
CTTTATGTAA TGAAGGGTCC CCGCTGGGAT ACCAATTTGT TTGGGCAGCA TGGAGTCACT 
GTTCACAGTC TTCTCAGCGA GCTCTATGGA AAAGCTGGCC TAAACCAAGA ATGGAGTTTG 
ATTCGCTACA TTTCAGGCCT GCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGTGCA 
GATCTGCTGT CCCACCAGAA GCAGCTTACA GTAGGACTGC CCCCTGAGCC CCGGGAGAAG 
ACCATATCTA CGCCTCTACC CCCAGAGGAG CTCACAGAAC TCATATATGA AGCCAGTGGA 
CAGGACATCA GCATTGCTGT CCTCACACAG GAGATCGTGG TTTACCTGGC CATGTATGTC 
CGGGCCCAGC CTAGCCTCTT TGCAGAGATG CTCAGACTCC GGATTGGATT GATCATCCAG 
GTGATGGCCA CAGAGCTGGC TCGGAGCCTG AACTGCTCAG GAAAAGAAGC TTCGGAGAGC 
TTGATGAACC TCAGCCCTTT CGACATGAAG AGCCTTCTGC ATCATATCCT GAGTGGGAAG 
GAGTTCGGTG TGGAGAGAAG CGTGCGCCCA ATACACTCCT CCATGTCCAG CCCTGCCATC 
TCCATCCATG AGGTGGGTCA TACTGGAGCC ACCAAAACTG AGCGGAGTGG CATCACCAGA 
CTGAGGAGTG AGATGAAACA GATGAATAGG CGAGCTAGTG CTGATGAGCA GTTCTTTCCT 
TTGGGCCAGA CCATGTCCAA CAGTTTGCAT TCCATCAAGT CTGTGAGGTC CAGCACCCCA 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC AGCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CCGGAGAAGG TTGGATGGGG CCATTAACAG GGTCCCCGTG 
GGATTCTACC AGAAAGTGTG GAAGATCCTT CAGAAGTGCC ATGGCCTGTC CATCGATGGT 
TACGTCCTCC CATCCTCGAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTTGCCGTC 
CATGTGGAGT CAGTGCTCAA CCGTGTGTCC CAGCCTGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACTCT GCTCTCAGAT ACCGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTGGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ACATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCTA GTGGGGCTTA TGGTACAATG ACCTACCTAA CAAAAGCGGT CGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAG TTCAGGCTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( NM_172783.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766371.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 1, mRNA.

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CloneID	OMu13343
Clone ID Related Accession (Same CDS sequence)	NM_172783.3 , NM_172783.4 , NM_001177879.1 , NM_001177879.2
Accession Version	NM_172783.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-07
Organism	Mus musculus(House mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB662591.1 and AK052346.1. On Apr 30, 2010 this sequence version replaced NM_172783.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode the same isoform. Sequence Note: An in-frame AUG is located 5 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052346.1, SRR1660823.130521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766371.1
CDS	394..4101
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	NM_172783.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172783.3

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( NM_172783.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766371.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 1, mRNA.

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CloneID	OMu13343
Clone ID Related Accession (Same CDS sequence)	NM_172783.3 , NM_172783.4 , NM_001177879.1 , NM_001177879.2
Accession Version	NM_172783.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731801.11, BB662591.1 and AK052346.1. On Dec 15, 2017 this sequence version replaced NM_172783.3. Sequence Note: An in-frame AUG is located 5 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052346.1, SRR1660825.130521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766371.1
CDS	666..4373
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	NM_172783.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172783.4

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( NM_001177879.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171350.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13343
Clone ID Related Accession (Same CDS sequence)	NM_172783.3 , NM_172783.4 , NM_001177879.1 , NM_001177879.2
Accession Version	NM_001177879.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-07
Organism	Mus musculus(House mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK165506.1 and AK052346.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Sequence Note: An in-frame AUG is located 5 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK165506.1, BC050040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171350.1
CDS	208..3915
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	NM_001177879.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177879.1

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( NM_001177879.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171350.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13343
Clone ID Related Accession (Same CDS sequence)	NM_172783.3 , NM_172783.4 , NM_001177879.1 , NM_001177879.2
Accession Version	NM_001177879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731801.11, AK165506.1 and AK052346.1. On Dec 15, 2017 this sequence version replaced NM_001177879.1. Sequence Note: An in-frame AUG is located 5 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK165506.1, BC050040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171350.1
CDS	212..3919
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	NM_001177879.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177879.2

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT ACAATGACCT ACCTAACAAA AGCGGTCGCT 
TCTCATTTGC AGGAACTGCT GCCCAGTTCA GGCTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( NM_001358966.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345895.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 4, mRNA.

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CloneID	OMu83674
Clone ID Related Accession (Same CDS sequence)	NM_001358966.1
Accession Version	NM_001358966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731801.11 and AL732450.5. On Dec 15, 2017 this sequence version replaced XM_006528680.3. Sequence Note: An in-frame AUG is located 5 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.36417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345895.1
CDS	666..4301
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGV

Target ORF information:

RefSeq Version	NM_001358966.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358966.1

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCATTTGGAA CCACAGACAT CCTGGAAAAA GACCAAGCCA CAGGAATTTG CCACCTCTTT 
TATGACAGTG CTCCTAGTGG GGCTTATGGT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_006528679.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528742.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56570
Clone ID Related Accession (Same CDS sequence)	XM_006528679.4 , XM_006528679.3
Accession Version	XM_006528679.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528679.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG GCGAGATGCG GAGCAGGAGC AATTCTGGGG TCCGCCTGGA CGGGTACGCG 
AGGCTGGTGC AGCAAACCAT CCTGTGTTAC CAGAACCCAG TCACTGGGCT GTTATCAGCC 
AGCCATGACC AGAAGGATGC CTGGGTACGG GACAATATCT ATAGCATATT GGCTGTTTGG 
GGCCTGGGCA TGGCCTACCG CAAGAATGCT GACCGTGATG AGGATAAAGC CAAGGCCTAT 
GAGTTGGAAC AGAACGTAGT GAAGCTGATG CGAGGTCTTC TCCAGTGTAT GATGAGGCAG 
GTGGACAAAG TGGAGAAGTT CAAGCACACT CAGAGTACCA AGGACAGTCT ACATGCCAAG 
TACAACACTG CCACATGTAG CACTGTGGTG GGTGATGACC AGTGGGGCCA CCTCCAGGTG 
GATGCCACCT CTCTCTTCCT CCTGTTCCTG GCCCAGATGA CAGCCTCAGG TTTGCGTATT 
ATTTTTACCC TTGACGAAGT GGCCTTCATA CAGAATCTTG TCTTCTACAT AGAAGCTGCA 
TATAAAGTTG CTGATTATGG GATGTGGGAG CGTGGAGATA AAACCAATCA GGGCATTCCG 
GAACTGAATG CAAGCTCGGT GGGAGTGGCC AAGGCAGCAC TTGAGGCCAT TGATGAACTA 
GATCTTTTTG GAGCCCATGG AGGACGCAAA TCGGTGATCC ACGTCCTACC TGATGAAGTT 
GAGCACTGCC AGTCAATTCT TTTCTCCATG TTGCCAAGAG CATCAACATC TAAAGAAATC 
GATGCTGGAC TTCTTTCTAT TATTTCTTTC CCGGCCTTTG CAGTAGAAGA TGTGAACCTT 
GTGAATGTGA CCAAAAATGA AATTATTTCC AAGCTTCAGG GGCGGTATGG ATGCTGTCGC 
TTCCTTCGAG ATGGCTATAA AACCCCAAGA GAGGATCCAC ACCGATTGCA TTATGACCCT 
GCCGAACTTA AGCTCTTTGA AAACATTGAA TGTGAATGGC CTGTGTTCTG GACTTATTTA 
ATCATAGATG GAATCTTCAA TGGTGATGCT GTTCAGGTCC AAGAATACCG AGAAGCCTTG 
GAGGGAATAT TAATCAGAGG CAAAGATGGG ATCCACTTGG TGCCAGAACT CTATGCCATC 
CCACCAGACA AGGTGGATGA AGAGTATAAG AATCCACACA CAGTAGACCG AGTTCCACTG 
GGAAAGCTGC CCCATCTTTG GGGACAGTCC TTGTACATCC TCAGCTCACT GCTAGCAGAG 
GGATTCCTTG CCACGGGTGA AATCGATCCC TTAAATAGAA GATTTTCTAC TTCAGTCAAA 
CCTGATGTTG TAGTGCAAGT TGCTGTTTTG GCAGAAAACA GTCACATTAA GGGGCTGTTG 
AAGGAGCATG GAATGACTGT CCAGAGCATT GCTGATGTGC ATCCGATTCG AGTCCAGCCA 
GGCCGAATTC TTAGTCACAT ATATGCCAAA CTTGGACGAA ATAAGAATAT GAAATTGAGT 
GGTCGACCGT ATCGGCACAT TGGTGTCCTT GGCACCTCTA AACTCTATGT GATTAGGAAT 
CACATCTTCA CTTTTACACC CCAGTTCACT GACCAGCATC ACTTCTACCT GGCCCTGGAC 
AATGAGATGA TTGTGGAGAT GCTGAGGATC GAACTTGCCT ATCTGTGCAC CTGCTGGCGG 
ATGACCGGCC GACCCACTCT CACCTTCCCT GTCACGCATA CCATGCTCAC AAATGATGGA 
TCAGACATTC ATCCTGCAGT TCTTTCTACA ATTAGAAAAC TAGAAGATGG CTATTTTGGA 
GGTGCTAGAG TAAAACTAGG AAATCTGGCA GAGTTCCTTA CTACCTCATT CTACACACAC 
CTGACCTTCC TGGATCCAGA CTGCGATGAG AAGTTGTTTG GTGACATCAC TGATAGGAGC 
TTTAGTCCTG ACAGTGAGCC AGACCTGGGA GGATACCTGG AAGACAGCAG TCCTCAAGAA 
AGCCAAGATG AACTTGACCA GTATATCAGC CACCTTCTTC AAAGCACATC CTTGAAGTGT 
TACCTGCCCC CTCTTTGTAA GAAGTCAGAA GACAGCCATT TTTTCAGTGC TATTCACTCC 
ACTCGGGACA TACTTTCTGT GATGGCCAAA GCGAAGGGTT TGGAAACTAC ATTTTTTCCC 
ATGATTTTGC CAACTAAAGT TCTAAGTGGA CACCGTAAGT CACTGAATCT TGTTGACTCC 
CCTCAGCCAC TCCTAAAGAC GACTCCTGAA TATGACTACC AGTGGCCCAG AGACGACCAT 
GATGAAGTGG ACTGTGAGAA GCTAGTTGGG CAACTGAAAG ACTGCTCAAA CCTACAGGAC 
CAAGCAGACA TTCTGTACAT TCTTTATGTA ATGAAGGGTC CCCGCTGGGA TACCAATTTG 
TTTGGGCAGC ATGGAGTCAC TGTTCACAGT CTTCTCAGCG AGCTCTATGG AAAAGCTGGC 
CTAAACCAAG AATGGAGTTT GATTCGCTAC ATTTCAGGCC TGCTCAGGAA GAAAGTGGAG 
GTCCTGGCTG AGGCCTGTGC AGATCTGCTG TCCCACCAGA AGCAGCTTAC AGTAGGACTG 
CCCCCTGAGC CCCGGGAGAA GACCATATCT ACGCCTCTAC CCCCAGAGGA GCTCACAGAA 
CTCATATATG AAGCCAGTGG ACAGGACATC AGCATTGCTG TCCTCACACA GGAGATCGTG 
GTTTACCTGG CCATGTATGT CCGGGCCCAG CCTAGCCTCT TTGCAGAGAT GCTCAGACTC 
CGGATTGGAT TGATCATCCA GGTGATGGCC ACAGAGCTGG CTCGGAGCCT GAACTGCTCA 
GGAAAAGAAG CTTCGGAGAG CTTGATGAAC CTCAGCCCTT TCGACATGAA GAGCCTTCTG 
CATCATATCC TGAGTGGGAA GGAGTTCGGT GTGGAGAGAA GCGTGCGCCC AATACACTCC 
TCCATGTCCA GCCCTGCCAT CTCCATCCAT GAGGTGGGTC ATACTGGAGC CACCAAAACT 
GAGCGGAGTG GCATCACCAG ACTGAGGAGT GAGATGAAAC AGATGAATAG GCGAGCTAGT 
GCTGATGAGC AGTTCTTTCC TTTGGGCCAG ACCATGTCCA ACAGTTTGCA TTCCATCAAG 
TCTGTGAGGT CCAGCACCCC ATCCTCCCCG ACAGGCACAT CATCTACAGA CTCTGGAGGA 
CAGCACCTGG GCTGGGGAGA ACAGCAGGGC CAGTGGCTGC GCCGGAGAAG GTTGGATGGG 
GCCATTAACA GGGTCCCCGT GGGATTCTAC CAGAAAGTGT GGAAGATCCT TCAGAAGTGC 
CATGGCCTGT CCATCGATGG TTACGTCCTC CCATCCTCGA CAACCCAAGA GATGACCCCT 
TGTGAGATCA AGTTTGCCGT CCATGTGGAG TCAGTGCTCA ACCGTGTGTC CCAGCCTGAG 
TATCGGCAGC TACTGGTAGA AGCCATCATG GTACTGACTC TGCTCTCAGA TACCGAAATG 
GACAGTATCG GGGGCATCAT CCATGTGGAC CAGATAGTGC AGCTGGCCAA TCAGCTGTTC 
CTGCAGGACC AGGTGTCATT TGGAACCACA GACATCCTGG AAAAAGACCA AGCCACAGGA 
ATTTGCCACC TCTTTTATGA CAGTGCTCCT AGTGGGGCTT ATGGTACAAT GACCTACCTA 
ACAAAAGCGG TCGCTTCTCA TTTGCAGGAA CTGCTGCCCA GTTCAGGCTG CCAGATGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528742.1
CDS	197..3919
Translation	MSLGEMRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	XM_006528679.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528679.4

ORF Insert Sequence:

ATGAGCTTGG GCGAGATGCG GAGCAGGAGC AATTCTGGGG TCCGCCTGGA CGGGTACGCG 
AGGCTGGTGC AGCAAACCAT CCTGTGTTAC CAGAACCCAG TCACTGGGCT GTTATCAGCC 
AGCCATGACC AGAAGGATGC CTGGGTACGG GACAATATCT ATAGCATATT GGCTGTTTGG 
GGCCTGGGCA TGGCCTACCG CAAGAATGCT GACCGTGATG AGGATAAAGC CAAGGCCTAT 
GAGTTGGAAC AGAACGTAGT GAAGCTGATG CGAGGTCTTC TCCAGTGTAT GATGAGGCAG 
GTGGACAAAG TGGAGAAGTT CAAGCACACT CAGAGTACCA AGGACAGTCT ACATGCCAAG 
TACAACACTG CCACATGTAG CACTGTGGTG GGTGATGACC AGTGGGGCCA CCTCCAGGTG 
GATGCCACCT CTCTCTTCCT CCTGTTCCTG GCCCAGATGA CAGCCTCAGG TTTGCGTATT 
ATTTTTACCC TTGACGAAGT GGCCTTCATA CAGAATCTTG TCTTCTACAT AGAAGCTGCA 
TATAAAGTTG CTGATTATGG GATGTGGGAG CGTGGAGATA AAACCAATCA GGGCATTCCG 
GAACTGAATG CAAGCTCGGT GGGAGTGGCC AAGGCAGCAC TTGAGGCCAT TGATGAACTA 
GATCTTTTTG GAGCCCATGG AGGACGCAAA TCGGTGATCC ACGTCCTACC TGATGAAGTT 
GAGCACTGCC AGTCAATTCT TTTCTCCATG TTGCCAAGAG CATCAACATC TAAAGAAATC 
GATGCTGGAC TTCTTTCTAT TATTTCTTTC CCGGCCTTTG CAGTAGAAGA TGTGAACCTT 
GTGAATGTGA CCAAAAATGA AATTATTTCC AAGCTTCAGG GGCGGTATGG ATGCTGTCGC 
TTCCTTCGAG ATGGCTATAA AACCCCAAGA GAGGATCCAC ACCGATTGCA TTATGACCCT 
GCCGAACTTA AGCTCTTTGA AAACATTGAA TGTGAATGGC CTGTGTTCTG GACTTATTTA 
ATCATAGATG GAATCTTCAA TGGTGATGCT GTTCAGGTCC AAGAATACCG AGAAGCCTTG 
GAGGGAATAT TAATCAGAGG CAAAGATGGG ATCCACTTGG TGCCAGAACT CTATGCCATC 
CCACCAGACA AGGTGGATGA AGAGTATAAG AATCCACACA CAGTAGACCG AGTTCCACTG 
GGAAAGCTGC CCCATCTTTG GGGACAGTCC TTGTACATCC TCAGCTCACT GCTAGCAGAG 
GGATTCCTTG CCACGGGTGA AATCGATCCC TTAAATAGAA GATTTTCTAC TTCAGTCAAA 
CCTGATGTTG TAGTGCAAGT TGCTGTTTTG GCAGAAAACA GTCACATTAA GGGGCTGTTG 
AAGGAGCATG GAATGACTGT CCAGAGCATT GCTGATGTGC ATCCGATTCG AGTCCAGCCA 
GGCCGAATTC TTAGTCACAT ATATGCCAAA CTTGGACGAA ATAAGAATAT GAAATTGAGT 
GGTCGACCGT ATCGGCACAT TGGTGTCCTT GGCACCTCTA AACTCTATGT GATTAGGAAT 
CACATCTTCA CTTTTACACC CCAGTTCACT GACCAGCATC ACTTCTACCT GGCCCTGGAC 
AATGAGATGA TTGTGGAGAT GCTGAGGATC GAACTTGCCT ATCTGTGCAC CTGCTGGCGG 
ATGACCGGCC GACCCACTCT CACCTTCCCT GTCACGCATA CCATGCTCAC AAATGATGGA 
TCAGACATTC ATCCTGCAGT TCTTTCTACA ATTAGAAAAC TAGAAGATGG CTATTTTGGA 
GGTGCTAGAG TAAAACTAGG AAATCTGGCA GAGTTCCTTA CTACCTCATT CTACACACAC 
CTGACCTTCC TGGATCCAGA CTGCGATGAG AAGTTGTTTG GTGACATCAC TGATAGGAGC 
TTTAGTCCTG ACAGTGAGCC AGACCTGGGA GGATACCTGG AAGACAGCAG TCCTCAAGAA 
AGCCAAGATG AACTTGACCA GTATATCAGC CACCTTCTTC AAAGCACATC CTTGAAGTGT 
TACCTGCCCC CTCTTTGTAA GAAGTCAGAA GACAGCCATT TTTTCAGTGC TATTCACTCC 
ACTCGGGACA TACTTTCTGT GATGGCCAAA GCGAAGGGTT TGGAAACTAC ATTTTTTCCC 
ATGATTTTGC CAACTAAAGT TCTAAGTGGA CACCGTAAGT CACTGAATCT TGTTGACTCC 
CCTCAGCCAC TCCTAAAGAC GACTCCTGAA TATGACTACC AGTGGCCCAG AGACGACCAT 
GATGAAGTGG ACTGTGAGAA GCTAGTTGGG CAACTGAAAG ACTGCTCAAA CCTACAGGAC 
CAAGCAGACA TTCTGTACAT TCTTTATGTA ATGAAGGGTC CCCGCTGGGA TACCAATTTG 
TTTGGGCAGC ATGGAGTCAC TGTTCACAGT CTTCTCAGCG AGCTCTATGG AAAAGCTGGC 
CTAAACCAAG AATGGAGTTT GATTCGCTAC ATTTCAGGCC TGCTCAGGAA GAAAGTGGAG 
GTCCTGGCTG AGGCCTGTGC AGATCTGCTG TCCCACCAGA AGCAGCTTAC AGTAGGACTG 
CCCCCTGAGC CCCGGGAGAA GACCATATCT ACGCCTCTAC CCCCAGAGGA GCTCACAGAA 
CTCATATATG AAGCCAGTGG ACAGGACATC AGCATTGCTG TCCTCACACA GGAGATCGTG 
GTTTACCTGG CCATGTATGT CCGGGCCCAG CCTAGCCTCT TTGCAGAGAT GCTCAGACTC 
CGGATTGGAT TGATCATCCA GGTGATGGCC ACAGAGCTGG CTCGGAGCCT GAACTGCTCA 
GGAAAAGAAG CTTCGGAGAG CTTGATGAAC CTCAGCCCTT TCGACATGAA GAGCCTTCTG 
CATCATATCC TGAGTGGGAA GGAGTTCGGT GTGGAGAGAA GCGTGCGCCC AATACACTCC 
TCCATGTCCA GCCCTGCCAT CTCCATCCAT GAGGTGGGTC ATACTGGAGC CACCAAAACT 
GAGCGGAGTG GCATCACCAG ACTGAGGAGT GAGATGAAAC AGATGAATAG GCGAGCTAGT 
GCTGATGAGC AGTTCTTTCC TTTGGGCCAG ACCATGTCCA ACAGTTTGCA TTCCATCAAG 
TCTGTGAGGT CCAGCACCCC ATCCTCCCCG ACAGGCACAT CATCTACAGA CTCTGGAGGA 
CAGCACCTGG GCTGGGGAGA ACAGCAGGGC CAGTGGCTGC GCCGGAGAAG GTTGGATGGG 
GCCATTAACA GGGTCCCCGT GGGATTCTAC CAGAAAGTGT GGAAGATCCT TCAGAAGTGC 
CATGGCCTGT CCATCGATGG TTACGTCCTC CCATCCTCGA CAACCCAAGA GATGACCCCT 
TGTGAGATCA AGTTTGCCGT CCATGTGGAG TCAGTGCTCA ACCGTGTGTC CCAGCCTGAG 
TATCGGCAGC TACTGGTAGA AGCCATCATG GTACTGACTC TGCTCTCAGA TACCGAAATG 
GACAGTATCG GGGGCATCAT CCATGTGGAC CAGATAGTGC AGCTGGCCAA TCAGCTGTTC 
CTGCAGGACC AGGTGTCATT TGGAACCACA GACATCCTGG AAAAAGACCA AGCCACAGGA 
ATTTGCCACC TCTTTTATGA CAGTGCTCCT AGTGGGGCTT ATGGTACAAT GACCTACCTA 
ACAAAAGCGG TCGCTTCTCA TTTGCAGGAA CTGCTGCCCA GTTCAGGCTG CCAGATGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_030251193.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107053.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu56573
Clone ID Related Accession (Same CDS sequence)	XM_006528682.1 , XM_030251193.1 , XM_030251194.1
Accession Version	XM_030251193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGAGC GTGGAGATAA AACCAATCAG GGCATTCCGG AACTGAATGC AAGCTCGGTG 
GGAGTGGCCA AGGCAGCACT TGAGGCCATT GATGAACTAG ATCTTTTTGG AGCCCATGGA 
GGACGCAAAT CGGTGATCCA CGTCCTACCT GATGAAGTTG AGCACTGCCA GTCAATTCTT 
TTCTCCATGT TGCCAAGAGC ATCAACATCT AAAGAAATCG ATGCTGGACT TCTTTCTATT 
ATTTCTTTCC CGGCCTTTGC AGTAGAAGAT GTGAACCTTG TGAATGTGAC CAAAAATGAA 
ATTATTTCCA AGCTTCAGGG GCGGTATGGA TGCTGTCGCT TCCTTCGAGA TGGCTATAAA 
ACCCCAAGAG AGGATCCACA CCGATTGCAT TATGACCCTG CCGAACTTAA GCTCTTTGAA 
AACATTGAAT GTGAATGGCC TGTGTTCTGG ACTTATTTAA TCATAGATGG AATCTTCAAT 
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAAGCCTTGG AGGGAATATT AATCAGAGGC 
AAAGATGGGA TCCACTTGGT GCCAGAACTC TATGCCATCC CACCAGACAA GGTGGATGAA 
GAGTATAAGA ATCCACACAC AGTAGACCGA GTTCCACTGG GAAAGCTGCC CCATCTTTGG 
GGACAGTCCT TGTACATCCT CAGCTCACTG CTAGCAGAGG GATTCCTTGC CACGGGTGAA 
ATCGATCCCT TAAATAGAAG ATTTTCTACT TCAGTCAAAC CTGATGTTGT AGTGCAAGTT 
GCTGTTTTGG CAGAAAACAG TCACATTAAG GGGCTGTTGA AGGAGCATGG AATGACTGTC 
CAGAGCATTG CTGATGTGCA TCCGATTCGA GTCCAGCCAG GCCGAATTCT TAGTCACATA 
TATGCCAAAC TTGGACGAAA TAAGAATATG AAATTGAGTG GTCGACCGTA TCGGCACATT 
GGTGTCCTTG GCACCTCTAA ACTCTATGTG ATTAGGAATC ACATCTTCAC TTTTACACCC 
CAGTTCACTG ACCAGCATCA CTTCTACCTG GCCCTGGACA ATGAGATGAT TGTGGAGATG 
CTGAGGATCG AACTTGCCTA TCTGTGCACC TGCTGGCGGA TGACCGGCCG ACCCACTCTC 
ACCTTCCCTG TCACGCATAC CATGCTCACA AATGATGGAT CAGACATTCA TCCTGCAGTT 
CTTTCTACAA TTAGAAAACT AGAAGATGGC TATTTTGGAG GTGCTAGAGT AAAACTAGGA 
AATCTGGCAG AGTTCCTTAC TACCTCATTC TACACACACC TGACCTTCCT GGATCCAGAC 
TGCGATGAGA AGTTGTTTGG TGACATCACT GATAGGAGCT TTAGTCCTGA CAGTGAGCCA 
GACCTGGGAG GATACCTGGA AGACAGCAGT CCTCAAGAAA GCCAAGATGA ACTTGACCAG 
TATATCAGCC ACCTTCTTCA AAGCACATCC TTGAAGTGTT ACCTGCCCCC TCTTTGTAAG 
AAGTCAGAAG ACAGCCATTT TTTCAGTGCT ATTCACTCCA CTCGGGACAT ACTTTCTGTG 
ATGGCCAAAG CGAAGGGTTT GGAAACTACA TTTTTTCCCA TGATTTTGCC AACTAAAGTT 
CTAAGTGGAC ACCGTAAGTC ACTGAATCTT GTTGACTCCC CTCAGCCACT CCTAAAGACG 
ACTCCTGAAT ATGACTACCA GTGGCCCAGA GACGACCATG ATGAAGTGGA CTGTGAGAAG 
CTAGTTGGGC AACTGAAAGA CTGCTCAAAC CTACAGGACC AAGCAGACAT TCTGTACATT 
CTTTATGTAA TGAAGGGTCC CCGCTGGGAT ACCAATTTGT TTGGGCAGCA TGGAGTCACT 
GTTCACAGTC TTCTCAGCGA GCTCTATGGA AAAGCTGGCC TAAACCAAGA ATGGAGTTTG 
ATTCGCTACA TTTCAGGCCT GCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGTGCA 
GATCTGCTGT CCCACCAGAA GCAGCTTACA GTAGGACTGC CCCCTGAGCC CCGGGAGAAG 
ACCATATCTA CGCCTCTACC CCCAGAGGAG CTCACAGAAC TCATATATGA AGCCAGTGGA 
CAGGACATCA GCATTGCTGT CCTCACACAG GAGATCGTGG TTTACCTGGC CATGTATGTC 
CGGGCCCAGC CTAGCCTCTT TGCAGAGATG CTCAGACTCC GGATTGGATT GATCATCCAG 
GTGATGGCCA CAGAGCTGGC TCGGAGCCTG AACTGCTCAG GAAAAGAAGC TTCGGAGAGC 
TTGATGAACC TCAGCCCTTT CGACATGAAG AGCCTTCTGC ATCATATCCT GAGTGGGAAG 
GAGTTCGGTG TGGAGAGAAG CGTGCGCCCA ATACACTCCT CCATGTCCAG CCCTGCCATC 
TCCATCCATG AGGTGGGTCA TACTGGAGCC ACCAAAACTG AGCGGAGTGG CATCACCAGA 
CTGAGGAGTG AGATGAAACA GATGAATAGG CGAGCTAGTG CTGATGAGCA GTTCTTTCCT 
TTGGGCCAGA CCATGTCCAA CAGTTTGCAT TCCATCAAGT CTGTGAGGTC CAGCACCCCA 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC AGCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CCGGAGAAGG TTGGATGGGG CCATTAACAG GGTCCCCGTG 
GGATTCTACC AGAAAGTGTG GAAGATCCTT CAGAAGTGCC ATGGCCTGTC CATCGATGGT 
TACGTCCTCC CATCCTCGAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTTGCCGTC 
CATGTGGAGT CAGTGCTCAA CCGTGTGTCC CAGCCTGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACTCT GCTCTCAGAT ACCGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTGGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ACATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCTA GTGGGGCTTA TGGTACAATG ACCTACCTAA CAAAAGCGGT CGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAG TTCAGGCTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107053.1
CDS	1821..4982
Translation	MWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	XM_030251193.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251193.1

ORF Insert Sequence:

ATGTGGGAGC GTGGAGATAA AACCAATCAG GGCATTCCGG AACTGAATGC AAGCTCGGTG 
GGAGTGGCCA AGGCAGCACT TGAGGCCATT GATGAACTAG ATCTTTTTGG AGCCCATGGA 
GGACGCAAAT CGGTGATCCA CGTCCTACCT GATGAAGTTG AGCACTGCCA GTCAATTCTT 
TTCTCCATGT TGCCAAGAGC ATCAACATCT AAAGAAATCG ATGCTGGACT TCTTTCTATT 
ATTTCTTTCC CGGCCTTTGC AGTAGAAGAT GTGAACCTTG TGAATGTGAC CAAAAATGAA 
ATTATTTCCA AGCTTCAGGG GCGGTATGGA TGCTGTCGCT TCCTTCGAGA TGGCTATAAA 
ACCCCAAGAG AGGATCCACA CCGATTGCAT TATGACCCTG CCGAACTTAA GCTCTTTGAA 
AACATTGAAT GTGAATGGCC TGTGTTCTGG ACTTATTTAA TCATAGATGG AATCTTCAAT 
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAAGCCTTGG AGGGAATATT AATCAGAGGC 
AAAGATGGGA TCCACTTGGT GCCAGAACTC TATGCCATCC CACCAGACAA GGTGGATGAA 
GAGTATAAGA ATCCACACAC AGTAGACCGA GTTCCACTGG GAAAGCTGCC CCATCTTTGG 
GGACAGTCCT TGTACATCCT CAGCTCACTG CTAGCAGAGG GATTCCTTGC CACGGGTGAA 
ATCGATCCCT TAAATAGAAG ATTTTCTACT TCAGTCAAAC CTGATGTTGT AGTGCAAGTT 
GCTGTTTTGG CAGAAAACAG TCACATTAAG GGGCTGTTGA AGGAGCATGG AATGACTGTC 
CAGAGCATTG CTGATGTGCA TCCGATTCGA GTCCAGCCAG GCCGAATTCT TAGTCACATA 
TATGCCAAAC TTGGACGAAA TAAGAATATG AAATTGAGTG GTCGACCGTA TCGGCACATT 
GGTGTCCTTG GCACCTCTAA ACTCTATGTG ATTAGGAATC ACATCTTCAC TTTTACACCC 
CAGTTCACTG ACCAGCATCA CTTCTACCTG GCCCTGGACA ATGAGATGAT TGTGGAGATG 
CTGAGGATCG AACTTGCCTA TCTGTGCACC TGCTGGCGGA TGACCGGCCG ACCCACTCTC 
ACCTTCCCTG TCACGCATAC CATGCTCACA AATGATGGAT CAGACATTCA TCCTGCAGTT 
CTTTCTACAA TTAGAAAACT AGAAGATGGC TATTTTGGAG GTGCTAGAGT AAAACTAGGA 
AATCTGGCAG AGTTCCTTAC TACCTCATTC TACACACACC TGACCTTCCT GGATCCAGAC 
TGCGATGAGA AGTTGTTTGG TGACATCACT GATAGGAGCT TTAGTCCTGA CAGTGAGCCA 
GACCTGGGAG GATACCTGGA AGACAGCAGT CCTCAAGAAA GCCAAGATGA ACTTGACCAG 
TATATCAGCC ACCTTCTTCA AAGCACATCC TTGAAGTGTT ACCTGCCCCC TCTTTGTAAG 
AAGTCAGAAG ACAGCCATTT TTTCAGTGCT ATTCACTCCA CTCGGGACAT ACTTTCTGTG 
ATGGCCAAAG CGAAGGGTTT GGAAACTACA TTTTTTCCCA TGATTTTGCC AACTAAAGTT 
CTAAGTGGAC ACCGTAAGTC ACTGAATCTT GTTGACTCCC CTCAGCCACT CCTAAAGACG 
ACTCCTGAAT ATGACTACCA GTGGCCCAGA GACGACCATG ATGAAGTGGA CTGTGAGAAG 
CTAGTTGGGC AACTGAAAGA CTGCTCAAAC CTACAGGACC AAGCAGACAT TCTGTACATT 
CTTTATGTAA TGAAGGGTCC CCGCTGGGAT ACCAATTTGT TTGGGCAGCA TGGAGTCACT 
GTTCACAGTC TTCTCAGCGA GCTCTATGGA AAAGCTGGCC TAAACCAAGA ATGGAGTTTG 
ATTCGCTACA TTTCAGGCCT GCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGTGCA 
GATCTGCTGT CCCACCAGAA GCAGCTTACA GTAGGACTGC CCCCTGAGCC CCGGGAGAAG 
ACCATATCTA CGCCTCTACC CCCAGAGGAG CTCACAGAAC TCATATATGA AGCCAGTGGA 
CAGGACATCA GCATTGCTGT CCTCACACAG GAGATCGTGG TTTACCTGGC CATGTATGTC 
CGGGCCCAGC CTAGCCTCTT TGCAGAGATG CTCAGACTCC GGATTGGATT GATCATCCAG 
GTGATGGCCA CAGAGCTGGC TCGGAGCCTG AACTGCTCAG GAAAAGAAGC TTCGGAGAGC 
TTGATGAACC TCAGCCCTTT CGACATGAAG AGCCTTCTGC ATCATATCCT GAGTGGGAAG 
GAGTTCGGTG TGGAGAGAAG CGTGCGCCCA ATACACTCCT CCATGTCCAG CCCTGCCATC 
TCCATCCATG AGGTGGGTCA TACTGGAGCC ACCAAAACTG AGCGGAGTGG CATCACCAGA 
CTGAGGAGTG AGATGAAACA GATGAATAGG CGAGCTAGTG CTGATGAGCA GTTCTTTCCT 
TTGGGCCAGA CCATGTCCAA CAGTTTGCAT TCCATCAAGT CTGTGAGGTC CAGCACCCCA 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC AGCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CCGGAGAAGG TTGGATGGGG CCATTAACAG GGTCCCCGTG 
GGATTCTACC AGAAAGTGTG GAAGATCCTT CAGAAGTGCC ATGGCCTGTC CATCGATGGT 
TACGTCCTCC CATCCTCGAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTTGCCGTC 
CATGTGGAGT CAGTGCTCAA CCGTGTGTCC CAGCCTGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACTCT GCTCTCAGAT ACCGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTGGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ACATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCTA GTGGGGCTTA TGGTACAATG ACCTACCTAA CAAAAGCGGT CGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAG TTCAGGCTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_030251194.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107054.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X6, mRNA.

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CloneID	OMu56573
Clone ID Related Accession (Same CDS sequence)	XM_006528682.1 , XM_030251193.1 , XM_030251194.1
Accession Version	XM_030251194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGAGC GTGGAGATAA AACCAATCAG GGCATTCCGG AACTGAATGC AAGCTCGGTG 
GGAGTGGCCA AGGCAGCACT TGAGGCCATT GATGAACTAG ATCTTTTTGG AGCCCATGGA 
GGACGCAAAT CGGTGATCCA CGTCCTACCT GATGAAGTTG AGCACTGCCA GTCAATTCTT 
TTCTCCATGT TGCCAAGAGC ATCAACATCT AAAGAAATCG ATGCTGGACT TCTTTCTATT 
ATTTCTTTCC CGGCCTTTGC AGTAGAAGAT GTGAACCTTG TGAATGTGAC CAAAAATGAA 
ATTATTTCCA AGCTTCAGGG GCGGTATGGA TGCTGTCGCT TCCTTCGAGA TGGCTATAAA 
ACCCCAAGAG AGGATCCACA CCGATTGCAT TATGACCCTG CCGAACTTAA GCTCTTTGAA 
AACATTGAAT GTGAATGGCC TGTGTTCTGG ACTTATTTAA TCATAGATGG AATCTTCAAT 
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAAGCCTTGG AGGGAATATT AATCAGAGGC 
AAAGATGGGA TCCACTTGGT GCCAGAACTC TATGCCATCC CACCAGACAA GGTGGATGAA 
GAGTATAAGA ATCCACACAC AGTAGACCGA GTTCCACTGG GAAAGCTGCC CCATCTTTGG 
GGACAGTCCT TGTACATCCT CAGCTCACTG CTAGCAGAGG GATTCCTTGC CACGGGTGAA 
ATCGATCCCT TAAATAGAAG ATTTTCTACT TCAGTCAAAC CTGATGTTGT AGTGCAAGTT 
GCTGTTTTGG CAGAAAACAG TCACATTAAG GGGCTGTTGA AGGAGCATGG AATGACTGTC 
CAGAGCATTG CTGATGTGCA TCCGATTCGA GTCCAGCCAG GCCGAATTCT TAGTCACATA 
TATGCCAAAC TTGGACGAAA TAAGAATATG AAATTGAGTG GTCGACCGTA TCGGCACATT 
GGTGTCCTTG GCACCTCTAA ACTCTATGTG ATTAGGAATC ACATCTTCAC TTTTACACCC 
CAGTTCACTG ACCAGCATCA CTTCTACCTG GCCCTGGACA ATGAGATGAT TGTGGAGATG 
CTGAGGATCG AACTTGCCTA TCTGTGCACC TGCTGGCGGA TGACCGGCCG ACCCACTCTC 
ACCTTCCCTG TCACGCATAC CATGCTCACA AATGATGGAT CAGACATTCA TCCTGCAGTT 
CTTTCTACAA TTAGAAAACT AGAAGATGGC TATTTTGGAG GTGCTAGAGT AAAACTAGGA 
AATCTGGCAG AGTTCCTTAC TACCTCATTC TACACACACC TGACCTTCCT GGATCCAGAC 
TGCGATGAGA AGTTGTTTGG TGACATCACT GATAGGAGCT TTAGTCCTGA CAGTGAGCCA 
GACCTGGGAG GATACCTGGA AGACAGCAGT CCTCAAGAAA GCCAAGATGA ACTTGACCAG 
TATATCAGCC ACCTTCTTCA AAGCACATCC TTGAAGTGTT ACCTGCCCCC TCTTTGTAAG 
AAGTCAGAAG ACAGCCATTT TTTCAGTGCT ATTCACTCCA CTCGGGACAT ACTTTCTGTG 
ATGGCCAAAG CGAAGGGTTT GGAAACTACA TTTTTTCCCA TGATTTTGCC AACTAAAGTT 
CTAAGTGGAC ACCGTAAGTC ACTGAATCTT GTTGACTCCC CTCAGCCACT CCTAAAGACG 
ACTCCTGAAT ATGACTACCA GTGGCCCAGA GACGACCATG ATGAAGTGGA CTGTGAGAAG 
CTAGTTGGGC AACTGAAAGA CTGCTCAAAC CTACAGGACC AAGCAGACAT TCTGTACATT 
CTTTATGTAA TGAAGGGTCC CCGCTGGGAT ACCAATTTGT TTGGGCAGCA TGGAGTCACT 
GTTCACAGTC TTCTCAGCGA GCTCTATGGA AAAGCTGGCC TAAACCAAGA ATGGAGTTTG 
ATTCGCTACA TTTCAGGCCT GCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGTGCA 
GATCTGCTGT CCCACCAGAA GCAGCTTACA GTAGGACTGC CCCCTGAGCC CCGGGAGAAG 
ACCATATCTA CGCCTCTACC CCCAGAGGAG CTCACAGAAC TCATATATGA AGCCAGTGGA 
CAGGACATCA GCATTGCTGT CCTCACACAG GAGATCGTGG TTTACCTGGC CATGTATGTC 
CGGGCCCAGC CTAGCCTCTT TGCAGAGATG CTCAGACTCC GGATTGGATT GATCATCCAG 
GTGATGGCCA CAGAGCTGGC TCGGAGCCTG AACTGCTCAG GAAAAGAAGC TTCGGAGAGC 
TTGATGAACC TCAGCCCTTT CGACATGAAG AGCCTTCTGC ATCATATCCT GAGTGGGAAG 
GAGTTCGGTG TGGAGAGAAG CGTGCGCCCA ATACACTCCT CCATGTCCAG CCCTGCCATC 
TCCATCCATG AGGTGGGTCA TACTGGAGCC ACCAAAACTG AGCGGAGTGG CATCACCAGA 
CTGAGGAGTG AGATGAAACA GATGAATAGG CGAGCTAGTG CTGATGAGCA GTTCTTTCCT 
TTGGGCCAGA CCATGTCCAA CAGTTTGCAT TCCATCAAGT CTGTGAGGTC CAGCACCCCA 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC AGCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CCGGAGAAGG TTGGATGGGG CCATTAACAG GGTCCCCGTG 
GGATTCTACC AGAAAGTGTG GAAGATCCTT CAGAAGTGCC ATGGCCTGTC CATCGATGGT 
TACGTCCTCC CATCCTCGAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTTGCCGTC 
CATGTGGAGT CAGTGCTCAA CCGTGTGTCC CAGCCTGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACTCT GCTCTCAGAT ACCGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTGGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ACATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCTA GTGGGGCTTA TGGTACAATG ACCTACCTAA CAAAAGCGGT CGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAG TTCAGGCTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107054.1
CDS	1146..4307
Translation	MWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ

Target ORF information:

RefSeq Version	XM_030251194.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251194.1

ORF Insert Sequence:

ATGTGGGAGC GTGGAGATAA AACCAATCAG GGCATTCCGG AACTGAATGC AAGCTCGGTG 
GGAGTGGCCA AGGCAGCACT TGAGGCCATT GATGAACTAG ATCTTTTTGG AGCCCATGGA 
GGACGCAAAT CGGTGATCCA CGTCCTACCT GATGAAGTTG AGCACTGCCA GTCAATTCTT 
TTCTCCATGT TGCCAAGAGC ATCAACATCT AAAGAAATCG ATGCTGGACT TCTTTCTATT 
ATTTCTTTCC CGGCCTTTGC AGTAGAAGAT GTGAACCTTG TGAATGTGAC CAAAAATGAA 
ATTATTTCCA AGCTTCAGGG GCGGTATGGA TGCTGTCGCT TCCTTCGAGA TGGCTATAAA 
ACCCCAAGAG AGGATCCACA CCGATTGCAT TATGACCCTG CCGAACTTAA GCTCTTTGAA 
AACATTGAAT GTGAATGGCC TGTGTTCTGG ACTTATTTAA TCATAGATGG AATCTTCAAT 
GGTGATGCTG TTCAGGTCCA AGAATACCGA GAAGCCTTGG AGGGAATATT AATCAGAGGC 
AAAGATGGGA TCCACTTGGT GCCAGAACTC TATGCCATCC CACCAGACAA GGTGGATGAA 
GAGTATAAGA ATCCACACAC AGTAGACCGA GTTCCACTGG GAAAGCTGCC CCATCTTTGG 
GGACAGTCCT TGTACATCCT CAGCTCACTG CTAGCAGAGG GATTCCTTGC CACGGGTGAA 
ATCGATCCCT TAAATAGAAG ATTTTCTACT TCAGTCAAAC CTGATGTTGT AGTGCAAGTT 
GCTGTTTTGG CAGAAAACAG TCACATTAAG GGGCTGTTGA AGGAGCATGG AATGACTGTC 
CAGAGCATTG CTGATGTGCA TCCGATTCGA GTCCAGCCAG GCCGAATTCT TAGTCACATA 
TATGCCAAAC TTGGACGAAA TAAGAATATG AAATTGAGTG GTCGACCGTA TCGGCACATT 
GGTGTCCTTG GCACCTCTAA ACTCTATGTG ATTAGGAATC ACATCTTCAC TTTTACACCC 
CAGTTCACTG ACCAGCATCA CTTCTACCTG GCCCTGGACA ATGAGATGAT TGTGGAGATG 
CTGAGGATCG AACTTGCCTA TCTGTGCACC TGCTGGCGGA TGACCGGCCG ACCCACTCTC 
ACCTTCCCTG TCACGCATAC CATGCTCACA AATGATGGAT CAGACATTCA TCCTGCAGTT 
CTTTCTACAA TTAGAAAACT AGAAGATGGC TATTTTGGAG GTGCTAGAGT AAAACTAGGA 
AATCTGGCAG AGTTCCTTAC TACCTCATTC TACACACACC TGACCTTCCT GGATCCAGAC 
TGCGATGAGA AGTTGTTTGG TGACATCACT GATAGGAGCT TTAGTCCTGA CAGTGAGCCA 
GACCTGGGAG GATACCTGGA AGACAGCAGT CCTCAAGAAA GCCAAGATGA ACTTGACCAG 
TATATCAGCC ACCTTCTTCA AAGCACATCC TTGAAGTGTT ACCTGCCCCC TCTTTGTAAG 
AAGTCAGAAG ACAGCCATTT TTTCAGTGCT ATTCACTCCA CTCGGGACAT ACTTTCTGTG 
ATGGCCAAAG CGAAGGGTTT GGAAACTACA TTTTTTCCCA TGATTTTGCC AACTAAAGTT 
CTAAGTGGAC ACCGTAAGTC ACTGAATCTT GTTGACTCCC CTCAGCCACT CCTAAAGACG 
ACTCCTGAAT ATGACTACCA GTGGCCCAGA GACGACCATG ATGAAGTGGA CTGTGAGAAG 
CTAGTTGGGC AACTGAAAGA CTGCTCAAAC CTACAGGACC AAGCAGACAT TCTGTACATT 
CTTTATGTAA TGAAGGGTCC CCGCTGGGAT ACCAATTTGT TTGGGCAGCA TGGAGTCACT 
GTTCACAGTC TTCTCAGCGA GCTCTATGGA AAAGCTGGCC TAAACCAAGA ATGGAGTTTG 
ATTCGCTACA TTTCAGGCCT GCTCAGGAAG AAAGTGGAGG TCCTGGCTGA GGCCTGTGCA 
GATCTGCTGT CCCACCAGAA GCAGCTTACA GTAGGACTGC CCCCTGAGCC CCGGGAGAAG 
ACCATATCTA CGCCTCTACC CCCAGAGGAG CTCACAGAAC TCATATATGA AGCCAGTGGA 
CAGGACATCA GCATTGCTGT CCTCACACAG GAGATCGTGG TTTACCTGGC CATGTATGTC 
CGGGCCCAGC CTAGCCTCTT TGCAGAGATG CTCAGACTCC GGATTGGATT GATCATCCAG 
GTGATGGCCA CAGAGCTGGC TCGGAGCCTG AACTGCTCAG GAAAAGAAGC TTCGGAGAGC 
TTGATGAACC TCAGCCCTTT CGACATGAAG AGCCTTCTGC ATCATATCCT GAGTGGGAAG 
GAGTTCGGTG TGGAGAGAAG CGTGCGCCCA ATACACTCCT CCATGTCCAG CCCTGCCATC 
TCCATCCATG AGGTGGGTCA TACTGGAGCC ACCAAAACTG AGCGGAGTGG CATCACCAGA 
CTGAGGAGTG AGATGAAACA GATGAATAGG CGAGCTAGTG CTGATGAGCA GTTCTTTCCT 
TTGGGCCAGA CCATGTCCAA CAGTTTGCAT TCCATCAAGT CTGTGAGGTC CAGCACCCCA 
TCCTCCCCGA CAGGCACATC ATCTACAGAC TCTGGAGGAC AGCACCTGGG CTGGGGAGAA 
CAGCAGGGCC AGTGGCTGCG CCGGAGAAGG TTGGATGGGG CCATTAACAG GGTCCCCGTG 
GGATTCTACC AGAAAGTGTG GAAGATCCTT CAGAAGTGCC ATGGCCTGTC CATCGATGGT 
TACGTCCTCC CATCCTCGAC AACCCAAGAG ATGACCCCTT GTGAGATCAA GTTTGCCGTC 
CATGTGGAGT CAGTGCTCAA CCGTGTGTCC CAGCCTGAGT ATCGGCAGCT ACTGGTAGAA 
GCCATCATGG TACTGACTCT GCTCTCAGAT ACCGAAATGG ACAGTATCGG GGGCATCATC 
CATGTGGACC AGATAGTGCA GCTGGCCAAT CAGCTGTTCC TGCAGGACCA GGTGTCATTT 
GGAACCACAG ACATCCTGGA AAAAGACCAA GCCACAGGAA TTTGCCACCT CTTTTATGAC 
AGTGCTCCTA GTGGGGCTTA TGGTACAATG ACCTACCTAA CAAAAGCGGT CGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAG TTCAGGCTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( NM_001177878.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171349.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 3, mRNA.

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CloneID	OMu13378
Clone ID Related Accession (Same CDS sequence)	NM_001177878.1 , NM_001177878.2
Accession Version	NM_001177878.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-07
Organism	Mus musculus(House mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform b
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052992.1. Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1, resulting in an isoform (b) with a distinct and shorter C-terminus, compared to isoform a. Sequence Note: An in-frame AUG is located 5 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCTGATCTGG ACAACTTGGC CATCAAAGTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171349.1
CDS	394..3969
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSDLDNLAIKVL

Target ORF information:

RefSeq Version	NM_001177878.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177878.1

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCTGATCTGG ACAACTTGGC CATCAAAGTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( NM_001177878.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171349.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 3, mRNA.

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CloneID	OMu13378
Clone ID Related Accession (Same CDS sequence)	NM_001177878.1 , NM_001177878.2
Accession Version	NM_001177878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731801.11 and AK052992.1. On Dec 15, 2017 this sequence version replaced NM_001177878.1. Sequence Note: An in-frame AUG is located 5 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCTGATCTGG ACAACTTGGC CATCAAAGTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171349.1
CDS	666..4241
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSDLDNLAIKVL

Target ORF information:

RefSeq Version	NM_001177878.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177878.2

ORF Insert Sequence:

ATGCGGAGCA GGAGCAATTC TGGGGTCCGC CTGGACGGGT ACGCGAGGCT GGTGCAGCAA 
ACCATCCTGT GTTACCAGAA CCCAGTCACT GGGCTGTTAT CAGCCAGCCA TGACCAGAAG 
GATGCCTGGG TACGGGACAA TATCTATAGC ATATTGGCTG TTTGGGGCCT GGGCATGGCC 
TACCGCAAGA ATGCTGACCG TGATGAGGAT AAAGCCAAGG CCTATGAGTT GGAACAGAAC 
GTAGTGAAGC TGATGCGAGG TCTTCTCCAG TGTATGATGA GGCAGGTGGA CAAAGTGGAG 
AAGTTCAAGC ACACTCAGAG TACCAAGGAC AGTCTACATG CCAAGTACAA CACTGCCACA 
TGTAGCACTG TGGTGGGTGA TGACCAGTGG GGCCACCTCC AGGTGGATGC CACCTCTCTC 
TTCCTCCTGT TCCTGGCCCA GATGACAGCC TCAGGTTTGC GTATTATTTT TACCCTTGAC 
GAAGTGGCCT TCATACAGAA TCTTGTCTTC TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGGATGT GGGAGCGTGG AGATAAAACC AATCAGGGCA TTCCGGAACT GAATGCAAGC 
TCGGTGGGAG TGGCCAAGGC AGCACTTGAG GCCATTGATG AACTAGATCT TTTTGGAGCC 
CATGGAGGAC GCAAATCGGT GATCCACGTC CTACCTGATG AAGTTGAGCA CTGCCAGTCA 
ATTCTTTTCT CCATGTTGCC AAGAGCATCA ACATCTAAAG AAATCGATGC TGGACTTCTT 
TCTATTATTT CTTTCCCGGC CTTTGCAGTA GAAGATGTGA ACCTTGTGAA TGTGACCAAA 
AATGAAATTA TTTCCAAGCT TCAGGGGCGG TATGGATGCT GTCGCTTCCT TCGAGATGGC 
TATAAAACCC CAAGAGAGGA TCCACACCGA TTGCATTATG ACCCTGCCGA ACTTAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTAATCAT AGATGGAATC 
TTCAATGGTG ATGCTGTTCA GGTCCAAGAA TACCGAGAAG CCTTGGAGGG AATATTAATC 
AGAGGCAAAG ATGGGATCCA CTTGGTGCCA GAACTCTATG CCATCCCACC AGACAAGGTG 
GATGAAGAGT ATAAGAATCC ACACACAGTA GACCGAGTTC CACTGGGAAA GCTGCCCCAT 
CTTTGGGGAC AGTCCTTGTA CATCCTCAGC TCACTGCTAG CAGAGGGATT CCTTGCCACG 
GGTGAAATCG ATCCCTTAAA TAGAAGATTT TCTACTTCAG TCAAACCTGA TGTTGTAGTG 
CAAGTTGCTG TTTTGGCAGA AAACAGTCAC ATTAAGGGGC TGTTGAAGGA GCATGGAATG 
ACTGTCCAGA GCATTGCTGA TGTGCATCCG ATTCGAGTCC AGCCAGGCCG AATTCTTAGT 
CACATATATG CCAAACTTGG ACGAAATAAG AATATGAAAT TGAGTGGTCG ACCGTATCGG 
CACATTGGTG TCCTTGGCAC CTCTAAACTC TATGTGATTA GGAATCACAT CTTCACTTTT 
ACACCCCAGT TCACTGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATTGTG 
GAGATGCTGA GGATCGAACT TGCCTATCTG TGCACCTGCT GGCGGATGAC CGGCCGACCC 
ACTCTCACCT TCCCTGTCAC GCATACCATG CTCACAAATG ATGGATCAGA CATTCATCCT 
GCAGTTCTTT CTACAATTAG AAAACTAGAA GATGGCTATT TTGGAGGTGC TAGAGTAAAA 
CTAGGAAATC TGGCAGAGTT CCTTACTACC TCATTCTACA CACACCTGAC CTTCCTGGAT 
CCAGACTGCG ATGAGAAGTT GTTTGGTGAC ATCACTGATA GGAGCTTTAG TCCTGACAGT 
GAGCCAGACC TGGGAGGATA CCTGGAAGAC AGCAGTCCTC AAGAAAGCCA AGATGAACTT 
GACCAGTATA TCAGCCACCT TCTTCAAAGC ACATCCTTGA AGTGTTACCT GCCCCCTCTT 
TGTAAGAAGT CAGAAGACAG CCATTTTTTC AGTGCTATTC ACTCCACTCG GGACATACTT 
TCTGTGATGG CCAAAGCGAA GGGTTTGGAA ACTACATTTT TTCCCATGAT TTTGCCAACT 
AAAGTTCTAA GTGGACACCG TAAGTCACTG AATCTTGTTG ACTCCCCTCA GCCACTCCTA 
AAGACGACTC CTGAATATGA CTACCAGTGG CCCAGAGACG ACCATGATGA AGTGGACTGT 
GAGAAGCTAG TTGGGCAACT GAAAGACTGC TCAAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTTT ATGTAATGAA GGGTCCCCGC TGGGATACCA ATTTGTTTGG GCAGCATGGA 
GTCACTGTTC ACAGTCTTCT CAGCGAGCTC TATGGAAAAG CTGGCCTAAA CCAAGAATGG 
AGTTTGATTC GCTACATTTC AGGCCTGCTC AGGAAGAAAG TGGAGGTCCT GGCTGAGGCC 
TGTGCAGATC TGCTGTCCCA CCAGAAGCAG CTTACAGTAG GACTGCCCCC TGAGCCCCGG 
GAGAAGACCA TATCTACGCC TCTACCCCCA GAGGAGCTCA CAGAACTCAT ATATGAAGCC 
AGTGGACAGG ACATCAGCAT TGCTGTCCTC ACACAGGAGA TCGTGGTTTA CCTGGCCATG 
TATGTCCGGG CCCAGCCTAG CCTCTTTGCA GAGATGCTCA GACTCCGGAT TGGATTGATC 
ATCCAGGTGA TGGCCACAGA GCTGGCTCGG AGCCTGAACT GCTCAGGAAA AGAAGCTTCG 
GAGAGCTTGA TGAACCTCAG CCCTTTCGAC ATGAAGAGCC TTCTGCATCA TATCCTGAGT 
GGGAAGGAGT TCGGTGTGGA GAGAAGCGTG CGCCCAATAC ACTCCTCCAT GTCCAGCCCT 
GCCATCTCCA TCCATGAGGT GGGTCATACT GGAGCCACCA AAACTGAGCG GAGTGGCATC 
ACCAGACTGA GGAGTGAGAT GAAACAGATG AATAGGCGAG CTAGTGCTGA TGAGCAGTTC 
TTTCCTTTGG GCCAGACCAT GTCCAACAGT TTGCATTCCA TCAAGTCTGT GAGGTCCAGC 
ACCCCATCCT CCCCGACAGG CACATCATCT ACAGACTCTG GAGGACAGCA CCTGGGCTGG 
GGAGAACAGC AGGGCCAGTG GCTGCGCCGG AGAAGGTTGG ATGGGGCCAT TAACAGGGTC 
CCCGTGGGAT TCTACCAGAA AGTGTGGAAG ATCCTTCAGA AGTGCCATGG CCTGTCCATC 
GATGGTTACG TCCTCCCATC CTCGACAACC CAAGAGATGA CCCCTTGTGA GATCAAGTTT 
GCCGTCCATG TGGAGTCAGT GCTCAACCGT GTGTCCCAGC CTGAGTATCG GCAGCTACTG 
GTAGAAGCCA TCATGGTACT GACTCTGCTC TCAGATACCG AAATGGACAG TATCGGGGGC 
ATCATCCATG TGGACCAGAT AGTGCAGCTG GCCAATCAGC TGTTCCTGCA GGACCAGGTG 
TCTGATCTGG ACAACTTGGC CATCAAAGTC CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka2 ( XM_006528680.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528743.1 Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X2, mRNA.

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CloneID	OMu56571
Clone ID Related Accession (Same CDS sequence)	XM_006528680.3
Accession Version	XM_006528680.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG GCGAGATGCG GAGCAGGAGC AATTCTGGGG TCCGCCTGGA CGGGTACGCG 
AGGCTGGTGC AGCAAACCAT CCTGTGTTAC CAGAACCCAG TCACTGGGCT GTTATCAGCC 
AGCCATGACC AGAAGGATGC CTGGGTACGG GACAATATCT ATAGCATATT GGCTGTTTGG 
GGCCTGGGCA TGGCCTACCG CAAGAATGCT GACCGTGATG AGGATAAAGC CAAGGCCTAT 
GAGTTGGAAC AGAACGTAGT GAAGCTGATG CGAGGTCTTC TCCAGTGTAT GATGAGGCAG 
GTGGACAAAG TGGAGAAGTT CAAGCACACT CAGAGTACCA AGGACAGTCT ACATGCCAAG 
TACAACACTG CCACATGTAG CACTGTGGTG GGTGATGACC AGTGGGGCCA CCTCCAGGTG 
GATGCCACCT CTCTCTTCCT CCTGTTCCTG GCCCAGATGA CAGCCTCAGG TTTGCGTATT 
ATTTTTACCC TTGACGAAGT GGCCTTCATA CAGAATCTTG TCTTCTACAT AGAAGCTGCA 
TATAAAGTTG CTGATTATGG GATGTGGGAG CGTGGAGATA AAACCAATCA GGGCATTCCG 
GAACTGAATG CAAGCTCGGT GGGAGTGGCC AAGGCAGCAC TTGAGGCCAT TGATGAACTA 
GATCTTTTTG GAGCCCATGG AGGACGCAAA TCGGTGATCC ACGTCCTACC TGATGAAGTT 
GAGCACTGCC AGTCAATTCT TTTCTCCATG TTGCCAAGAG CATCAACATC TAAAGAAATC 
GATGCTGGAC TTCTTTCTAT TATTTCTTTC CCGGCCTTTG CAGTAGAAGA TGTGAACCTT 
GTGAATGTGA CCAAAAATGA AATTATTTCC AAGCTTCAGG GGCGGTATGG ATGCTGTCGC 
TTCCTTCGAG ATGGCTATAA AACCCCAAGA GAGGATCCAC ACCGATTGCA TTATGACCCT 
GCCGAACTTA AGCTCTTTGA AAACATTGAA TGTGAATGGC CTGTGTTCTG GACTTATTTA 
ATCATAGATG GAATCTTCAA TGGTGATGCT GTTCAGGTCC AAGAATACCG AGAAGCCTTG 
GAGGGAATAT TAATCAGAGG CAAAGATGGG ATCCACTTGG TGCCAGAACT CTATGCCATC 
CCACCAGACA AGGTGGATGA AGAGTATAAG AATCCACACA CAGTAGACCG AGTTCCACTG 
GGAAAGCTGC CCCATCTTTG GGGACAGTCC TTGTACATCC TCAGCTCACT GCTAGCAGAG 
GGATTCCTTG CCACGGGTGA AATCGATCCC TTAAATAGAA GATTTTCTAC TTCAGTCAAA 
CCTGATGTTG TAGTGCAAGT TGCTGTTTTG GCAGAAAACA GTCACATTAA GGGGCTGTTG 
AAGGAGCATG GAATGACTGT CCAGAGCATT GCTGATGTGC ATCCGATTCG AGTCCAGCCA 
GGCCGAATTC TTAGTCACAT ATATGCCAAA CTTGGACGAA ATAAGAATAT GAAATTGAGT 
GGTCGACCGT ATCGGCACAT TGGTGTCCTT GGCACCTCTA AACTCTATGT GATTAGGAAT 
CACATCTTCA CTTTTACACC CCAGTTCACT GACCAGCATC ACTTCTACCT GGCCCTGGAC 
AATGAGATGA TTGTGGAGAT GCTGAGGATC GAACTTGCCT ATCTGTGCAC CTGCTGGCGG 
ATGACCGGCC GACCCACTCT CACCTTCCCT GTCACGCATA CCATGCTCAC AAATGATGGA 
TCAGACATTC ATCCTGCAGT TCTTTCTACA ATTAGAAAAC TAGAAGATGG CTATTTTGGA 
GGTGCTAGAG TAAAACTAGG AAATCTGGCA GAGTTCCTTA CTACCTCATT CTACACACAC 
CTGACCTTCC TGGATCCAGA CTGCGATGAG AAGTTGTTTG GTGACATCAC TGATAGGAGC 
TTTAGTCCTG ACAGTGAGCC AGACCTGGGA GGATACCTGG AAGACAGCAG TCCTCAAGAA 
AGCCAAGATG AACTTGACCA GTATATCAGC CACCTTCTTC AAAGCACATC CTTGAAGTGT 
TACCTGCCCC CTCTTTGTAA GAAGTCAGAA GACAGCCATT TTTTCAGTGC TATTCACTCC 
ACTCGGGACA TACTTTCTGT GATGGCCAAA GCGAAGGGTT TGGAAACTAC ATTTTTTCCC 
ATGATTTTGC CAACTAAAGT TCTAAGTGGA CACCGTAAGT CACTGAATCT TGTTGACTCC 
CCTCAGCCAC TCCTAAAGAC GACTCCTGAA TATGACTACC AGTGGCCCAG AGACGACCAT 
GATGAAGTGG ACTGTGAGAA GCTAGTTGGG CAACTGAAAG ACTGCTCAAA CCTACAGGAC 
CAAGCAGACA TTCTGTACAT TCTTTATGTA ATGAAGGGTC CCCGCTGGGA TACCAATTTG 
TTTGGGCAGC ATGGAGTCAC TGTTCACAGT CTTCTCAGCG AGCTCTATGG AAAAGCTGGC 
CTAAACCAAG AATGGAGTTT GATTCGCTAC ATTTCAGGCC TGCTCAGGAA GAAAGTGGAG 
GTCCTGGCTG AGGCCTGTGC AGATCTGCTG TCCCACCAGA AGCAGCTTAC AGTAGGACTG 
CCCCCTGAGC CCCGGGAGAA GACCATATCT ACGCCTCTAC CCCCAGAGGA GCTCACAGAA 
CTCATATATG AAGCCAGTGG ACAGGACATC AGCATTGCTG TCCTCACACA GGAGATCGTG 
GTTTACCTGG CCATGTATGT CCGGGCCCAG CCTAGCCTCT TTGCAGAGAT GCTCAGACTC 
CGGATTGGAT TGATCATCCA GGTGATGGCC ACAGAGCTGG CTCGGAGCCT GAACTGCTCA 
GGAAAAGAAG CTTCGGAGAG CTTGATGAAC CTCAGCCCTT TCGACATGAA GAGCCTTCTG 
CATCATATCC TGAGTGGGAA GGAGTTCGGT GTGGAGAGAA GCGTGCGCCC AATACACTCC 
TCCATGTCCA GCCCTGCCAT CTCCATCCAT GAGGTGGGTC ATACTGGAGC CACCAAAACT 
GAGCGGAGTG GCATCACCAG ACTGAGGAGT GAGATGAAAC AGATGAATAG GCGAGCTAGT 
GCTGATGAGC AGTTCTTTCC TTTGGGCCAG ACCATGTCCA ACAGTTTGCA TTCCATCAAG 
TCTGTGAGGT CCAGCACCCC ATCCTCCCCG ACAGGCACAT CATCTACAGA CTCTGGAGGA 
CAGCACCTGG GCTGGGGAGA ACAGCAGGGC CAGTGGCTGC GCCGGAGAAG GTTGGATGGG 
GCCATTAACA GGGTCCCCGT GGGATTCTAC CAGAAAGTGT GGAAGATCCT TCAGAAGTGC 
CATGGCCTGT CCATCGATGG TTACGTCCTC CCATCCTCGA CAACCCAAGA GATGACCCCT 
TGTGAGATCA AGTTTGCCGT CCATGTGGAG TCAGTGCTCA ACCGTGTGTC CCAGCCTGAG 
TATCGGCAGC TACTGGTAGA AGCCATCATG GTACTGACTC TGCTCTCAGA TACCGAAATG 
GACAGTATCG GGGGCATCAT CCATGTGGAC CAGATAGTGC AGCTGGCCAA TCAGCTGTTC 
CTGCAGGACC AGGTGTCATT TGGAACCACA GACATCCTGG AAAAAGACCA AGCCACAGGA 
ATTTGCCACC TCTTTTATGA CAGTGCTCCT AGTGGGGCTT ATGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528743.1
CDS	658..4308
Translation	MSLGEMRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDAVQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAEGFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAKLGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDEKLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHSTRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVGQLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVEVLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHSSMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSPTGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPCEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTTDILEKDQATGICHLFYDSAPSGAYGV

Target ORF information:

RefSeq Version	XM_006528680.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus phosphorylase kinase alpha 2 (Phka2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528680.3

ORF Insert Sequence:

ATGAGCTTGG GCGAGATGCG GAGCAGGAGC AATTCTGGGG TCCGCCTGGA CGGGTACGCG 
AGGCTGGTGC AGCAAACCAT CCTGTGTTAC CAGAACCCAG TCACTGGGCT GTTATCAGCC 
AGCCATGACC AGAAGGATGC CTGGGTACGG GACAATATCT ATAGCATATT GGCTGTTTGG 
GGCCTGGGCA TGGCCTACCG CAAGAATGCT GACCGTGATG AGGATAAAGC CAAGGCCTAT 
GAGTTGGAAC AGAACGTAGT GAAGCTGATG CGAGGTCTTC TCCAGTGTAT GATGAGGCAG 
GTGGACAAAG TGGAGAAGTT CAAGCACACT CAGAGTACCA AGGACAGTCT ACATGCCAAG 
TACAACACTG CCACATGTAG CACTGTGGTG GGTGATGACC AGTGGGGCCA CCTCCAGGTG 
GATGCCACCT CTCTCTTCCT CCTGTTCCTG GCCCAGATGA CAGCCTCAGG TTTGCGTATT 
ATTTTTACCC TTGACGAAGT GGCCTTCATA CAGAATCTTG TCTTCTACAT AGAAGCTGCA 
TATAAAGTTG CTGATTATGG GATGTGGGAG CGTGGAGATA AAACCAATCA GGGCATTCCG 
GAACTGAATG CAAGCTCGGT GGGAGTGGCC AAGGCAGCAC TTGAGGCCAT TGATGAACTA 
GATCTTTTTG GAGCCCATGG AGGACGCAAA TCGGTGATCC ACGTCCTACC TGATGAAGTT 
GAGCACTGCC AGTCAATTCT TTTCTCCATG TTGCCAAGAG CATCAACATC TAAAGAAATC 
GATGCTGGAC TTCTTTCTAT TATTTCTTTC CCGGCCTTTG CAGTAGAAGA TGTGAACCTT 
GTGAATGTGA CCAAAAATGA AATTATTTCC AAGCTTCAGG GGCGGTATGG ATGCTGTCGC 
TTCCTTCGAG ATGGCTATAA AACCCCAAGA GAGGATCCAC ACCGATTGCA TTATGACCCT 
GCCGAACTTA AGCTCTTTGA AAACATTGAA TGTGAATGGC CTGTGTTCTG GACTTATTTA 
ATCATAGATG GAATCTTCAA TGGTGATGCT GTTCAGGTCC AAGAATACCG AGAAGCCTTG 
GAGGGAATAT TAATCAGAGG CAAAGATGGG ATCCACTTGG TGCCAGAACT CTATGCCATC 
CCACCAGACA AGGTGGATGA AGAGTATAAG AATCCACACA CAGTAGACCG AGTTCCACTG 
GGAAAGCTGC CCCATCTTTG GGGACAGTCC TTGTACATCC TCAGCTCACT GCTAGCAGAG 
GGATTCCTTG CCACGGGTGA AATCGATCCC TTAAATAGAA GATTTTCTAC TTCAGTCAAA 
CCTGATGTTG TAGTGCAAGT TGCTGTTTTG GCAGAAAACA GTCACATTAA GGGGCTGTTG 
AAGGAGCATG GAATGACTGT CCAGAGCATT GCTGATGTGC ATCCGATTCG AGTCCAGCCA 
GGCCGAATTC TTAGTCACAT ATATGCCAAA CTTGGACGAA ATAAGAATAT GAAATTGAGT 
GGTCGACCGT ATCGGCACAT TGGTGTCCTT GGCACCTCTA AACTCTATGT GATTAGGAAT 
CACATCTTCA CTTTTACACC CCAGTTCACT GACCAGCATC ACTTCTACCT GGCCCTGGAC 
AATGAGATGA TTGTGGAGAT GCTGAGGATC GAACTTGCCT ATCTGTGCAC CTGCTGGCGG 
ATGACCGGCC GACCCACTCT CACCTTCCCT GTCACGCATA CCATGCTCAC AAATGATGGA 
TCAGACATTC ATCCTGCAGT TCTTTCTACA ATTAGAAAAC TAGAAGATGG CTATTTTGGA 
GGTGCTAGAG TAAAACTAGG AAATCTGGCA GAGTTCCTTA CTACCTCATT CTACACACAC 
CTGACCTTCC TGGATCCAGA CTGCGATGAG AAGTTGTTTG GTGACATCAC TGATAGGAGC 
TTTAGTCCTG ACAGTGAGCC AGACCTGGGA GGATACCTGG AAGACAGCAG TCCTCAAGAA 
AGCCAAGATG AACTTGACCA GTATATCAGC CACCTTCTTC AAAGCACATC CTTGAAGTGT 
TACCTGCCCC CTCTTTGTAA GAAGTCAGAA GACAGCCATT TTTTCAGTGC TATTCACTCC 
ACTCGGGACA TACTTTCTGT GATGGCCAAA GCGAAGGGTT TGGAAACTAC ATTTTTTCCC 
ATGATTTTGC CAACTAAAGT TCTAAGTGGA CACCGTAAGT CACTGAATCT TGTTGACTCC 
CCTCAGCCAC TCCTAAAGAC GACTCCTGAA TATGACTACC AGTGGCCCAG AGACGACCAT 
GATGAAGTGG ACTGTGAGAA GCTAGTTGGG CAACTGAAAG ACTGCTCAAA CCTACAGGAC 
CAAGCAGACA TTCTGTACAT TCTTTATGTA ATGAAGGGTC CCCGCTGGGA TACCAATTTG 
TTTGGGCAGC ATGGAGTCAC TGTTCACAGT CTTCTCAGCG AGCTCTATGG AAAAGCTGGC 
CTAAACCAAG AATGGAGTTT GATTCGCTAC ATTTCAGGCC TGCTCAGGAA GAAAGTGGAG 
GTCCTGGCTG AGGCCTGTGC AGATCTGCTG TCCCACCAGA AGCAGCTTAC AGTAGGACTG 
CCCCCTGAGC CCCGGGAGAA GACCATATCT ACGCCTCTAC CCCCAGAGGA GCTCACAGAA 
CTCATATATG AAGCCAGTGG ACAGGACATC AGCATTGCTG TCCTCACACA GGAGATCGTG 
GTTTACCTGG CCATGTATGT CCGGGCCCAG CCTAGCCTCT TTGCAGAGAT GCTCAGACTC 
CGGATTGGAT TGATCATCCA GGTGATGGCC ACAGAGCTGG CTCGGAGCCT GAACTGCTCA 
GGAAAAGAAG CTTCGGAGAG CTTGATGAAC CTCAGCCCTT TCGACATGAA GAGCCTTCTG 
CATCATATCC TGAGTGGGAA GGAGTTCGGT GTGGAGAGAA GCGTGCGCCC AATACACTCC 
TCCATGTCCA GCCCTGCCAT CTCCATCCAT GAGGTGGGTC ATACTGGAGC CACCAAAACT 
GAGCGGAGTG GCATCACCAG ACTGAGGAGT GAGATGAAAC AGATGAATAG GCGAGCTAGT 
GCTGATGAGC AGTTCTTTCC TTTGGGCCAG ACCATGTCCA ACAGTTTGCA TTCCATCAAG 
TCTGTGAGGT CCAGCACCCC ATCCTCCCCG ACAGGCACAT CATCTACAGA CTCTGGAGGA 
CAGCACCTGG GCTGGGGAGA ACAGCAGGGC CAGTGGCTGC GCCGGAGAAG GTTGGATGGG 
GCCATTAACA GGGTCCCCGT GGGATTCTAC CAGAAAGTGT GGAAGATCCT TCAGAAGTGC 
CATGGCCTGT CCATCGATGG TTACGTCCTC CCATCCTCGA CAACCCAAGA GATGACCCCT 
TGTGAGATCA AGTTTGCCGT CCATGTGGAG TCAGTGCTCA ACCGTGTGTC CCAGCCTGAG 
TATCGGCAGC TACTGGTAGA AGCCATCATG GTACTGACTC TGCTCTCAGA TACCGAAATG 
GACAGTATCG GGGGCATCAT CCATGTGGAC CAGATAGTGC AGCTGGCCAA TCAGCTGTTC 
CTGCAGGACC AGGTGTCATT TGGAACCACA GACATCCTGG AAAAAGACCA AGCCACAGGA 
ATTTGCCACC TCTTTTATGA CAGTGCTCCT AGTGGGGCTT ATGGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The apical ectodermal ridge of the mouse model of ectrodactyly Dlx5;Dlx6-/- shows altered stratification and cell polarity, which are restored by exogenous Wnt5a ligand.
Human molecular genetics25(4)740-54(2016 Feb)
Conte D,Garaffo G,Lo Iacono N,Mantero S,Piccolo S,Cordenonsi M,Perez-Morga D,Orecchia V,Poli V,Merlo GR

Glycine N-methyltransferase-/- mice develop chronic hepatitis and glycogen storage disease in the liver.
Hepatology (Baltimore, Md.)46(5)1413-25(2007 Nov)
Liu SP,Li YS,Chen YJ,Chiang EP,Li AF,Lee YH,Tsai TF,Hsiao M,Huang SF,Hwang SF,Chen YM

GenePaint.org: an atlas of gene expression patterns in the mouse embryo.
Nucleic acids research32(Database issue)D552-6(2004 Jan)
Visel A,Thaller C,Eichele G

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Comparative FISH mapping of mouse and rat homologues of twenty-five human X-linked genes.
Cytogenetics and cell genetics81(3-4)208-12(1998)
Kuroiwa A,Watanabe T,Hishigaki H,Takahashi E,Namikawa T,Matsuda Y

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