Capza3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capza3
Entrez Gene ID	12344
Full Name	capping protein (actin filament) muscle Z-line, alpha 3
Synonyms	510-4,Cappa3,Gsg3,Tex8,repro32
General protein information	Preferred Names capping protein (actin filament) muscle Z-line, alpha 3 Names F-actin-capping protein subunit alpha-3 capZ alpha-3 capping protein alpha 3 cp alpha3 germ cell-specific protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 G2\|6 69.77 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007605.4	NP_031631.3	F-actin-capping protein subunit alpha-3
NM_007605.5	NP_031631.3	F-actin-capping protein subunit alpha-3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CAPZA3	NP_201585.1
	CAPZA3	XP_001096292.1
Mus musculus (house mouse)	Capza3	NP_031631.3
Gallus gallus (chicken)	CAPZA3	NP_001265047.1
Canis lupus familiaris (dog)	CAPZA3	XP_534875.1
Bos taurus (cattle)	CAPZA3	NP_001035651.1
Pan troglodytes (chimpanzee)	CAPZA3	NP_001233504.1
Rattus norvegicus (Norway rat)	Capza3	NP_058860.2

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of CAPZA3 localization reveals temporally discrete events during the acrosome reaction.
Sosnik J, Buffone MG, Visconti PE
Journal of cellular physiology224(3)575-80(2010 Sep)

A missense mutation in the Capza3 gene and disruption of F-actin organization in spermatids of repro32 infertile male mice.
Geyer CB, Inselman AL, Sunman JA, Bornstein S, Handel MA, Eddy EM
Developmental biology330(1)142-52(2009 Jun)

Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice.
Robledo RF, Ciciotte SL, Gwynn B, Sahr KE, Gilligan DM, Mohandas N, Peters LL
Blood112(10)4298-307(2008 Nov)

Characterizing mouse male germ cell-specific actin capping protein alpha3 (CPalpha3): dynamic patterns of expression in testicular and epididymal sperm.
Tokuhiro K, Miyagawa Y, Tanaka H
Asian journal of andrology10(5)711-8(2008 Sep)

Centimorgan-range one-step mapping of fertility traits using interspecific recombinant congenic mice.
L'H?te D, Serres C, Laissue P, Oulmouden A, Rogel-Gaillard C, Montagutelli X, Vaiman D
Genetics176(3)1907-21(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

to understand actin-mediated change in protein localization during acrosome reaction, distribution of CAPZA3 was analyzed; like IZUMO1, CAPZA3 shows dynamic pattern of localization; movements follow different temporal pattern than changes seen with IZUMO1
Title: Analysis of CAPZA3 localization reveals temporally discrete events during the acrosome reaction.

These observations strongly suggest the missense mutation in Capza3 is responsible for the mutant phenotype of repro32/repro32 sperm and regulation of F-actin dynamics
Title: A missense mutation in the Capza3 gene and disruption of F-actin organization in spermatids of repro32 infertile male mice.

CPalpha3 might play an important role in sperm morphogenesis and/or sperm function
Title: Characterizing mouse male germ cell-specific actin capping protein alpha3 (CPalpha3): dynamic patterns of expression in testicular and epididymal sperm.

The following Capza3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capza3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu58447	NM_007605.5 Latest version!	Mus musculus capping protein (actin filament) muscle Z-line, alpha 3 (Capza3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu58447	NM_007605.4	Mus musculus capping protein (actin filament) muscle Z-line, alpha 3 (Capza3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capza3 ( NM_007605.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031631.3 Mus musculus capping protein (actin filament) muscle Z-line, alpha 3 (Capza3), mRNA.

Price & Availability↑

CloneID	OMu58447
Clone ID Related Accession (Same CDS sequence)	NM_007605.4 , NM_007605.5
Accession Version	NM_007605.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-11
Organism	Mus musculus(house mouse)
Product	F-actin-capping protein subunit alpha-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY707143.1, BC049620.1, AK016391.1 and BX635805.1. On Oct 6, 2007 this sequence version replaced NM_007605.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGTCACTCA GCGTCTTGAG TAGGAAAGAG AAAGAGAAAG TCATCCACAG GCTCTTAGTC 
CAGGCTCCTC CTGGGGAATT TGTGAATGCC TTTGACGATC TCTGTCTGCT TATCCGAGAT 
GAAAAGCTTA TGCACCATCA GGGTGAGTGC GCAGGCCATC AGCACTGCCA AAAATACTGT 
GTCCCACTCT GCATCGACGG CAACCCAGTC CTCCTGTCTC ACCACAATGT GATGGGTGAC 
TTCCGCTTCT TTGACTACCA GAGCAAACTT TCTTTCAGGT TTGACCTACT CCAGAATCAA 
CTGAGGGATA TCCAAAGTCA CGGAATCATT CGGAATGAGA CCGAGTACCT AAGATCCGTT 
GTTATGTGTG CCTTAAAACT CTATGTGAAT GATCACTACC CAAATGGAAA TTGCAATGTG 
CTGAGAAAAA CAGTCAAAAG CAAAGAATTC TTAATAGCTT GCATTGAAGA CCACAGCTAT 
GACAATGGAG AGTGTTGGAA CGGTCTTTGG AAGTCTAAGT GGATCTTCCA GGTAAACCCG 
TTTCTAACCC AAGTCACAGG GAGAATTTTT GTGCAAGCTC ACTTCTTTAG GTGTGTCAAC 
CTTCATATTG AAGTCTCCAA GGACCTAAAG GAGAGCTTGG AGGTGGTCAA TCAAGCCCAG 
TTGGCTCTCA GTTTCGCAAG GCTTGTGGAA GAACAAGAGA ATAAGTTTCA AGCTGCTGTC 
ATAGAAGAAC TACAGGAGTT GTCTAACGAA GCCCTGAGAA AAATATTACG GAGGGATCTT 
CCAGTGACAC GCACTTTGAT TGACTGGCAA CGGATCCTCT CTGACTTGAA TCTGGTGATG 
TACCCCAAGT TAGGGTATGT TATTTACTCA AGAAGTGTGT TGTGCAACTG GATAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031631.3
CDS	153..1052
Translation	MSLSVLSRKEKEKVIHRLLVQAPPGEFVNAFDDLCLLIRDEKLMHHQGECAGHQHCQKYCVPLCIDGNPVLLSHHNVMGDFRFFDYQSKLSFRFDLLQNQLRDIQSHGIIRNETEYLRSVVMCALKLYVNDHYPNGNCNVLRKTVKSKEFLIACIEDHSYDNGECWNGLWKSKWIFQVNPFLTQVTGRIFVQAHFFRCVNLHIEVSKDLKESLEVVNQAQLALSFARLVEEQENKFQAAVIEELQELSNEALRKILRRDLPVTRTLIDWQRILSDLNLVMYPKLGYVIYSRSVLCNWII

Target ORF information:

RefSeq Version	NM_007605.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus capping protein (actin filament) muscle Z-line, alpha 3 (Capza3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007605.4

ORF Insert Sequence:

ATGTCACTCA GCGTCTTGAG TAGGAAAGAG AAAGAGAAAG TCATCCACAG GCTCTTAGTC 
CAGGCTCCTC CTGGGGAATT TGTGAATGCC TTTGACGATC TCTGTCTGCT TATCCGAGAT 
GAAAAGCTTA TGCACCATCA GGGTGAGTGC GCAGGCCATC AGCACTGCCA AAAATACTGT 
GTCCCACTCT GCATCGACGG CAACCCAGTC CTCCTGTCTC ACCACAATGT GATGGGTGAC 
TTCCGCTTCT TTGACTACCA GAGCAAACTT TCTTTCAGGT TTGACCTACT CCAGAATCAA 
CTGAGGGATA TCCAAAGTCA CGGAATCATT CGGAATGAGA CCGAGTACCT AAGATCCGTT 
GTTATGTGTG CCTTAAAACT CTATGTGAAT GATCACTACC CAAATGGAAA TTGCAATGTG 
CTGAGAAAAA CAGTCAAAAG CAAAGAATTC TTAATAGCTT GCATTGAAGA CCACAGCTAT 
GACAATGGAG AGTGTTGGAA CGGTCTTTGG AAGTCTAAGT GGATCTTCCA GGTAAACCCG 
TTTCTAACCC AAGTCACAGG GAGAATTTTT GTGCAAGCTC ACTTCTTTAG GTGTGTCAAC 
CTTCATATTG AAGTCTCCAA GGACCTAAAG GAGAGCTTGG AGGTGGTCAA TCAAGCCCAG 
TTGGCTCTCA GTTTCGCAAG GCTTGTGGAA GAACAAGAGA ATAAGTTTCA AGCTGCTGTC 
ATAGAAGAAC TACAGGAGTT GTCTAACGAA GCCCTGAGAA AAATATTACG GAGGGATCTT 
CCAGTGACAC GCACTTTGAT TGACTGGCAA CGGATCCTCT CTGACTTGAA TCTGGTGATG 
TACCCCAAGT TAGGGTATGT TATTTACTCA AGAAGTGTGT TGTGCAACTG GATAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capza3 ( NM_007605.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031631.3 Mus musculus capping protein (actin filament) muscle Z-line, alpha 3 (Capza3), mRNA.

Price & Availability↑

CloneID	OMu58447
Clone ID Related Accession (Same CDS sequence)	NM_007605.4 , NM_007605.5
Accession Version	NM_007605.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Mus musculus(house mouse)
Product	F-actin-capping protein subunit alpha-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158625.9. On Feb 8, 2019 this sequence version replaced NM_007605.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR5189685.155398.1, AK016391.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCACTCA GCGTCTTGAG TAGGAAAGAG AAAGAGAAAG TCATCCACAG GCTCTTAGTC 
CAGGCTCCTC CTGGGGAATT TGTGAATGCC TTTGACGATC TCTGTCTGCT TATCCGAGAT 
GAAAAGCTTA TGCACCATCA GGGTGAGTGC GCAGGCCATC AGCACTGCCA AAAATACTGT 
GTCCCACTCT GCATCGACGG CAACCCAGTC CTCCTGTCTC ACCACAATGT GATGGGTGAC 
TTCCGCTTCT TTGACTACCA GAGCAAACTT TCTTTCAGGT TTGACCTACT CCAGAATCAA 
CTGAGGGATA TCCAAAGTCA CGGAATCATT CGGAATGAGA CCGAGTACCT AAGATCCGTT 
GTTATGTGTG CCTTAAAACT CTATGTGAAT GATCACTACC CAAATGGAAA TTGCAATGTG 
CTGAGAAAAA CAGTCAAAAG CAAAGAATTC TTAATAGCTT GCATTGAAGA CCACAGCTAT 
GACAATGGAG AGTGTTGGAA CGGTCTTTGG AAGTCTAAGT GGATCTTCCA GGTAAACCCG 
TTTCTAACCC AAGTCACAGG GAGAATTTTT GTGCAAGCTC ACTTCTTTAG GTGTGTCAAC 
CTTCATATTG AAGTCTCCAA GGACCTAAAG GAGAGCTTGG AGGTGGTCAA TCAAGCCCAG 
TTGGCTCTCA GTTTCGCAAG GCTTGTGGAA GAACAAGAGA ATAAGTTTCA AGCTGCTGTC 
ATAGAAGAAC TACAGGAGTT GTCTAACGAA GCCCTGAGAA AAATATTACG GAGGGATCTT 
CCAGTGACAC GCACTTTGAT TGACTGGCAA CGGATCCTCT CTGACTTGAA TCTGGTGATG 
TACCCCAAGT TAGGGTATGT TATTTACTCA AGAAGTGTGT TGTGCAACTG GATAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031631.3
CDS	121..1020
Translation	MSLSVLSRKEKEKVIHRLLVQAPPGEFVNAFDDLCLLIRDEKLMHHQGECAGHQHCQKYCVPLCIDGNPVLLSHHNVMGDFRFFDYQSKLSFRFDLLQNQLRDIQSHGIIRNETEYLRSVVMCALKLYVNDHYPNGNCNVLRKTVKSKEFLIACIEDHSYDNGECWNGLWKSKWIFQVNPFLTQVTGRIFVQAHFFRCVNLHIEVSKDLKESLEVVNQAQLALSFARLVEEQENKFQAAVIEELQELSNEALRKILRRDLPVTRTLIDWQRILSDLNLVMYPKLGYVIYSRSVLCNWII

Target ORF information:

RefSeq Version	NM_007605.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus capping protein (actin filament) muscle Z-line, alpha 3 (Capza3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007605.5

ORF Insert Sequence:

ATGTCACTCA GCGTCTTGAG TAGGAAAGAG AAAGAGAAAG TCATCCACAG GCTCTTAGTC 
CAGGCTCCTC CTGGGGAATT TGTGAATGCC TTTGACGATC TCTGTCTGCT TATCCGAGAT 
GAAAAGCTTA TGCACCATCA GGGTGAGTGC GCAGGCCATC AGCACTGCCA AAAATACTGT 
GTCCCACTCT GCATCGACGG CAACCCAGTC CTCCTGTCTC ACCACAATGT GATGGGTGAC 
TTCCGCTTCT TTGACTACCA GAGCAAACTT TCTTTCAGGT TTGACCTACT CCAGAATCAA 
CTGAGGGATA TCCAAAGTCA CGGAATCATT CGGAATGAGA CCGAGTACCT AAGATCCGTT 
GTTATGTGTG CCTTAAAACT CTATGTGAAT GATCACTACC CAAATGGAAA TTGCAATGTG 
CTGAGAAAAA CAGTCAAAAG CAAAGAATTC TTAATAGCTT GCATTGAAGA CCACAGCTAT 
GACAATGGAG AGTGTTGGAA CGGTCTTTGG AAGTCTAAGT GGATCTTCCA GGTAAACCCG 
TTTCTAACCC AAGTCACAGG GAGAATTTTT GTGCAAGCTC ACTTCTTTAG GTGTGTCAAC 
CTTCATATTG AAGTCTCCAA GGACCTAAAG GAGAGCTTGG AGGTGGTCAA TCAAGCCCAG 
TTGGCTCTCA GTTTCGCAAG GCTTGTGGAA GAACAAGAGA ATAAGTTTCA AGCTGCTGTC 
ATAGAAGAAC TACAGGAGTT GTCTAACGAA GCCCTGAGAA AAATATTACG GAGGGATCTT 
CCAGTGACAC GCACTTTGAT TGACTGGCAA CGGATCCTCT CTGACTTGAA TCTGGTGATG 
TACCCCAAGT TAGGGTATGT TATTTACTCA AGAAGTGTGT TGTGCAACTG GATAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of CAPZA3 localization reveals temporally discrete events during the acrosome reaction.
Journal of cellular physiology224(3)575-80(2010 Sep)
Sosnik J,Buffone MG,Visconti PE

A missense mutation in the Capza3 gene and disruption of F-actin organization in spermatids of repro32 infertile male mice.
Developmental biology330(1)142-52(2009 Jun)
Geyer CB,Inselman AL,Sunman JA,Bornstein S,Handel MA,Eddy EM

Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice.
Blood112(10)4298-307(2008 Nov)
Robledo RF,Ciciotte SL,Gwynn B,Sahr KE,Gilligan DM,Mohandas N,Peters LL

Characterizing mouse male germ cell-specific actin capping protein alpha3 (CPalpha3): dynamic patterns of expression in testicular and epididymal sperm.
Asian journal of andrology10(5)711-8(2008 Sep)
Tokuhiro K,Miyagawa Y,Tanaka H

Centimorgan-range one-step mapping of fertility traits using interspecific recombinant congenic mice.
Genetics176(3)1907-21(2007 Jul)
L'H?te D,Serres C,Laissue P,Oulmouden A,Rogel-Gaillard C,Montagutelli X,Vaiman D