Hspa4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa4
Entrez Gene ID	15525
Full Name	heat shock protein 4
Synonyms	70kDa,AI317151,APG-2,Hsp110,Hsp70RY,mKIAA4025
General protein information	Preferred Names heat shock protein 4 Names heat shock 70 kDa protein 4 heat shock 70-related protein APG-2 heat shock protein, 110 kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B1.3\|11 31.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008300.3	NP_032326.3	heat shock 70 kDa protein 4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	HSPA4	XP_414655.2
Caenorhabditis elegans (roundworm)	C30C11.4	NP_498868.1
Saccharomyces cerevisiae (baker's yeast)	SSE1	NP_015219.1
Eremothecium gossypii	AGOS_AGR212W	NP_986878.1
Magnaporthe oryzae (rice blast fungus)	MGG_06065	XP_003711919.1
Arabidopsis thaliana (thale cress)	AT1G79920	NP_850984.4
Oryza sativa (rice)	Os05g0181000	NP_001054812.1
Homo sapiens (human)	HSPA4	NP_002145.3
Mus musculus (house mouse)	Hspa4	NP_032326.3
Danio rerio (zebrafish)	hspa4a	NP_999881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010331	XP_315285.3
Kluyveromyces lactis	KLLA0E24597g	XP_455059.1
Rattus norvegicus (Norway rat)	Hspa4	NP_705893.1
	HSPA4	XP_001106968.1
Danio rerio (zebrafish)	hspa4b	NP_956151.1
Drosophila melanogaster (fruit fly)	Hsc70Cb	NP_001189103.1
Schizosaccharomyces pombe (fission yeast)	pss1	XP_001713060.1
Xenopus tropicalis (tropical clawed frog)	hspa4	NP_989252.1
Pan troglodytes (chimpanzee)	HSPA4	XP_517930.2
Canis lupus familiaris (dog)	HSPA4	NP_001041481.1
Bos taurus (cattle)	HSPA4	NP_001107664.1
Neurospora crassa	NCU05269	XP_961759.2
Arabidopsis thaliana (thale cress)	HSP91	NP_178111.1
Oryza sativa (rice)	Os01g0180800	NP_001042210.1

Gene Ontology
Bibliography

Related articles in PubMed

Geranylgeranylacetone Suppresses N-Methyl-N-nitrosourea-Induced Photoreceptor Cell Loss in Mice.
Koriyama Y, Ogai K, Sugitani K, Hisano S, Kato S
Advances in experimental medicine and biology854237-43(2016)

Heat shock protein A4 controls cell migration and gastric ulcer healing.
Sakurai T, Kashida H, Hagiwara S, Nishida N, Watanabe T, Fujita J, Kudo M
Digestive diseases and sciences60(4)850-7(2015 Apr)

Involvement of heat shock protein a4/apg-2 in refractory inflammatory bowel disease.
Adachi T, Sakurai T, Kashida H, Mine H, Hagiwara S, Matsui S, Yoshida K, Nishida N, Watanabe T, Itoh K, Fujita J, Kudo M
Inflammatory bowel diseases21(1)31-9(2015 Jan)

Therapeutic inducers of the HSP70/HSP110 protect mice against traumatic brain injury.
Eroglu B, Kimbler DE, Pang J, Choi J, Moskophidis D, Yanasak N, Dhandapani KM, Mivechi NF
Journal of neurochemistry130(5)626-41(2014 Sep)

Assessment of the risk of blastomere biopsy during preimplantation genetic diagnosis in a mouse model: reducing female ovary function with an increase in age by proteomics method.
Yu Y, Zhao Y, Li R, Li L, Zhao H, Li M, Sha J, Zhou Q, Qiao J
Journal of proteome research12(12)5475-86(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that heat shock protein 70 (HSP70) induction by geranylgeranylacetone (GGA) was effective against N-methyl-N-nitrosourea (MNU-induced photoreceptor cell loss.
Title: Geranylgeranylacetone Suppresses N-Methyl-N-nitrosourea-Induced Photoreceptor Cell Loss in Mice.

regulates the expression of SDF-1 and Twist in fibroblasts, thereby controlling gastric ulcer healing
Title: Heat shock protein A4 controls cell migration and gastric ulcer healing.

Upregulation of HSPA4 would control apoptosis of inflammatory cells and immune response in the gut, which might develop treatment resistance in inflammatory bowel disease.
Title: Involvement of heat shock protein a4/apg-2 in refractory inflammatory bowel disease.

PSMB8, ALDH1A1, and HSPA4 were identified to be located in ovarian tissues, to regulate 12 cellular pathways, and demonstrate age-dependent dynamic changes in expression profiling.
Title: Assessment of the risk of blastomere biopsy during preimplantation genetic diagnosis in a mouse model: reducing female ovary function with an increase in age by proteomics method.

Inhibition of Apg-2 by shRNA not only decreased cell proliferation, but also induced cell apoptosis in BCR/ABL positive chronic myeloid leukemia cells (BaF3-BCR/ABL, BaF3-BCR/ABL(T315I)).
Title: Proteomic-based identification of Apg-2 as a therapeutic target for chronic myeloid leukemia.

The following Hspa4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14995	NM_008300.3 Latest version!	Mus musculus heat shock protein 4 (Hspa4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa4 ( NM_008300.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032326.3 Mus musculus heat shock protein 4 (Hspa4), mRNA.

Price & Availability↑

CloneID	OMu14995
Clone ID Related Accession (Same CDS sequence)	NM_008300.3
Accession Version	NM_008300.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	heat shock 70 kDa protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI316935.1, CF741432.1, CN458707.1, CA316420.1, AW545353.1, AL596382.12 and AA203833.1. On Aug 15, 2006 this sequence version replaced NM_008300.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK220167.1, AK166102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGTGG TGGGCATAGA CCTGGGCTTT CAGAGCTGCT ATGTCGCTGT GGCCCGCGCC 
GGCGGCATCG AGACGATCGC TAACGAGTAT AGCGACCGCT GCACGCCGGC ATGTGTTTCT 
TTTGGTCCTA AGAATCGTTC AATTGGAGCA GCAGCTAAGA GCCAGGTAAT TTCTAATGCC 
AAGAACACAG TTCAAGGCTT TAAAAGATTC CATGGGCGTG CATTCTCCGA TCCATTTGTG 
GAAGCAGAAA AATCTAATCT TGCATATGAT ATTGTGCAGT TGCCCACTGG ATTAACGGGC 
ATAAAGGTTA CATATATGGA AGAAGAGAGG AATTTTACCA CGGAACAAGT GACTGCCATG 
CTTTTGTCGA AACTGAAGGA GACAGCAGAG AGTGTTCTTA AGAAGCCTGT GGTAGACTGT 
GTTGTCTCGG TTCCTAGTTT CTACACTGAT GCAGAACGGC GGTCAGTGAT GGATGCCACA 
CAGATTGCTG GTCTCAATTG TTTGCGGTTA ATGAATGAAA CAACTGCAGT TGCTCTTGCT 
TATGGAATCT ATAAACAAGA CCTTCCTGCC TTAGAAGAAA AACCAAGAAA TGTAGTTTTT 
GTAGATATGG GACATTCTGC CTATCAAGTT TCTGTATGTG CATTTAATAA AGGAAAACTG 
AAAGTTTTGG CCACAGCTTT TGATACAACA TTGGGAGGCA GAAAATTTGA TGAGGTGTTG 
GTAAATCACT TCTGTGAAGA ATTTGGAAAG AAATACAAGC TAGACATAAA ATCTAAAATT 
CGTGCTTTAT TACGACTGTC TCAAGAGTGT GAGAAACTCA AGAAATTGAT GAGTGCGAAT 
GCTTCAGACC TCCCTTTGAG CATTGAGTGT TTTATGAATG ATATTGATGT GTCCGGAACT 
ATGAATAGAG GCAAATTTTT GGAGATGTGT GATGATCTCC TAGCAAGAGT GGAGCCGCCA 
CTTCGAAGCG TCTTGGAGCA ATCCAAGTTA AAGAAAGAAG ATATTTATGC AGTGGAGATA 
GTTGGTGGTG CCACACGAAT CCCTGCAGTG AAAGAGAAGA TCAGCAAGTT TTTTGGTAAA 
GAACTCAGCA CAACATTAAA TGCAGATGAA GCTGTCACTC GAGGCTGTGC TCTGCAGTGT 
GCAATTTTAT CACCTGCTTT CAAAGTCAGA GAATTTTCTA TCACTGATGT GGTACCATAT 
CCAATATCTT TGCGATGGAA TTCTCCAGCT GAAGAGGGGT TAAGTGACTG TGAAGTCTTC 
CCCAAAAACC ATGCTGCTCC TTTCTCCAAA GTCCTCACCT TCTACAGAAA GGAACCTTTC 
ACTCTTGAGG CCTACTATAG TTCTCCTCAG GATTTGCCCT ATCCAGATCC TGCTATAGCT 
CAGTTCTCAG TTCAGAAAGT CACTCCACAG TCTGATGGCT CCAGCTCAAA AGTGAAAGTC 
AAGGTCCGGG TGAACGTGCA CGGCATCTTC AGTGTGTCCA GTGCGGCCTT GGTGGAGGTG 
CACAAGTCTG AAGAGAGTGA GGAGCCCATG GAGACGGATC AGAATGCAAA GGAAGAGGAG 
AAGATGCAAG TGGACCAGGA AGAACCACAT ACTGAAGAGC AGCAGCAGCA GCCACAGACA 
CCAGCAGAAA ACAAGGCGGA GTCTGAGGAG ATGGAGACAT CTCAAGCTGG ATCAAAAGAT 
AAAAAGACGG ACCAACCACC TCAAGCAAAG AAGGCAAAAG TGAAGACCAG CACTGTGGAC 
CTGCCCATTG AGAGCCAGCT GCTCTGGCAG CTGGACCGGG AGATGCTCGG CCTGTACACA 
GAGAATGAGG GTAAGATGAT CATGCAGGAT AAACTGGAGA AGGAACGGAA TGACGCCAAG 
AATGCTGTGG AAGAGTATGT GTATGAGATG AGAGACAAGC TTAGTGGTGA ATATGAGAAG 
TTTGTGAGTG AAGATGATCG TAATACTTTT ACCTTAAAAT TAGAGGATAC TGAAAATTGG 
CTGTATGAAG ATGGAGAAGA TCAACCAAAG CAAGTTTATG TAGACAAATT GGCTGAATTA 
AAAAGTTTAG GTCAACCCAT TAAGACACGG TTCCAGGAAT CTGAAGAAAG ACCAAAATTA 
TTTGAAGAAC TAGGGAAGCA AATCCAACAG TATATGAAAG TGATCAGCTC TTTCAAAAAC 
AAGGAGGACC AGTATGAACA CTTGGATGCT GCTGACGTGA CAAAGGTAGA GAAAAGCACC 
AATGAGGCGA TGGAGTGGAT GAATAGCAAG CTTAACCTGC AGAACAAACA GAGCCTGACG 
GTGGATCCAG TTGTCAAGAC AAAGGAGATT GAAGCTAAAA TTAAGGAGCT GACAAGTATT 
TGCAGTCCTA TAATTTCAAA ACCCAAACCC AAAGTGGAAC CCCCAAAGGA GGAACCAAAA 
CATGCTGAGC AGAATGGACC AGTGGATGGC CAAGGAGACA ACCCAGGCTC CCAGGCTGCT 
GAGCACGGTG CAGACACAGC TGTGCCTTCT GATGGCGACA AGAAGCTTCC TGAGATGGAC 
ATTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032326.3
CDS	314..2842
Translation	MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACVSFGPKNRSIGAAAKSQVISNAKNTVQGFKRFHGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDCVVSVPSFYTDAERRSVMDATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQVSVCAFNKGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKIRALLRLSQECEKLKKLMSANASDLPLSIECFMNDIDVSGTMNRGKFLEMCDDLLARVEPPLRSVLEQSKLKKEDIYAVEIVGGATRIPAVKEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGLSDCEVFPKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIFSVSSAALVEVHKSEESEEPMETDQNAKEEEKMQVDQEEPHTEEQQQQPQTPAENKAESEEMETSQAGSKDKKTDQPPQAKKAKVKTSTVDLPIESQLLWQLDREMLGLYTENEGKMIMQDKLEKERNDAKNAVEEYVYEMRDKLSGEYEKFVSEDDRNTFTLKLEDTENWLYEDGEDQPKQVYVDKLAELKSLGQPIKTRFQESEERPKLFEELGKQIQQYMKVISSFKNKEDQYEHLDAADVTKVEKSTNEAMEWMNSKLNLQNKQSLTVDPVVKTKEIEAKIKELTSICSPIISKPKPKVEPPKEEPKHAEQNGPVDGQGDNPGSQAAEHGADTAVPSDGDKKLPEMDID

Target ORF information:

RefSeq Version	NM_008300.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock protein 4 (Hspa4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008300.3

ORF Insert Sequence:

ATGTCGGTGG TGGGCATAGA CCTGGGCTTT CAGAGCTGCT ATGTCGCTGT GGCCCGCGCC 
GGCGGCATCG AGACGATCGC TAACGAGTAT AGCGACCGCT GCACGCCGGC ATGTGTTTCT 
TTTGGTCCTA AGAATCGTTC AATTGGAGCA GCAGCTAAGA GCCAGGTAAT TTCTAATGCC 
AAGAACACAG TTCAAGGCTT TAAAAGATTC CATGGGCGTG CATTCTCCGA TCCATTTGTG 
GAAGCAGAAA AATCTAATCT TGCATATGAT ATTGTGCAGT TGCCCACTGG ATTAACGGGC 
ATAAAGGTTA CATATATGGA AGAAGAGAGG AATTTTACCA CGGAACAAGT GACTGCCATG 
CTTTTGTCGA AACTGAAGGA GACAGCAGAG AGTGTTCTTA AGAAGCCTGT GGTAGACTGT 
GTTGTCTCGG TTCCTAGTTT CTACACTGAT GCAGAACGGC GGTCAGTGAT GGATGCCACA 
CAGATTGCTG GTCTCAATTG TTTGCGGTTA ATGAATGAAA CAACTGCAGT TGCTCTTGCT 
TATGGAATCT ATAAACAAGA CCTTCCTGCC TTAGAAGAAA AACCAAGAAA TGTAGTTTTT 
GTAGATATGG GACATTCTGC CTATCAAGTT TCTGTATGTG CATTTAATAA AGGAAAACTG 
AAAGTTTTGG CCACAGCTTT TGATACAACA TTGGGAGGCA GAAAATTTGA TGAGGTGTTG 
GTAAATCACT TCTGTGAAGA ATTTGGAAAG AAATACAAGC TAGACATAAA ATCTAAAATT 
CGTGCTTTAT TACGACTGTC TCAAGAGTGT GAGAAACTCA AGAAATTGAT GAGTGCGAAT 
GCTTCAGACC TCCCTTTGAG CATTGAGTGT TTTATGAATG ATATTGATGT GTCCGGAACT 
ATGAATAGAG GCAAATTTTT GGAGATGTGT GATGATCTCC TAGCAAGAGT GGAGCCGCCA 
CTTCGAAGCG TCTTGGAGCA ATCCAAGTTA AAGAAAGAAG ATATTTATGC AGTGGAGATA 
GTTGGTGGTG CCACACGAAT CCCTGCAGTG AAAGAGAAGA TCAGCAAGTT TTTTGGTAAA 
GAACTCAGCA CAACATTAAA TGCAGATGAA GCTGTCACTC GAGGCTGTGC TCTGCAGTGT 
GCAATTTTAT CACCTGCTTT CAAAGTCAGA GAATTTTCTA TCACTGATGT GGTACCATAT 
CCAATATCTT TGCGATGGAA TTCTCCAGCT GAAGAGGGGT TAAGTGACTG TGAAGTCTTC 
CCCAAAAACC ATGCTGCTCC TTTCTCCAAA GTCCTCACCT TCTACAGAAA GGAACCTTTC 
ACTCTTGAGG CCTACTATAG TTCTCCTCAG GATTTGCCCT ATCCAGATCC TGCTATAGCT 
CAGTTCTCAG TTCAGAAAGT CACTCCACAG TCTGATGGCT CCAGCTCAAA AGTGAAAGTC 
AAGGTCCGGG TGAACGTGCA CGGCATCTTC AGTGTGTCCA GTGCGGCCTT GGTGGAGGTG 
CACAAGTCTG AAGAGAGTGA GGAGCCCATG GAGACGGATC AGAATGCAAA GGAAGAGGAG 
AAGATGCAAG TGGACCAGGA AGAACCACAT ACTGAAGAGC AGCAGCAGCA GCCACAGACA 
CCAGCAGAAA ACAAGGCGGA GTCTGAGGAG ATGGAGACAT CTCAAGCTGG ATCAAAAGAT 
AAAAAGACGG ACCAACCACC TCAAGCAAAG AAGGCAAAAG TGAAGACCAG CACTGTGGAC 
CTGCCCATTG AGAGCCAGCT GCTCTGGCAG CTGGACCGGG AGATGCTCGG CCTGTACACA 
GAGAATGAGG GTAAGATGAT CATGCAGGAT AAACTGGAGA AGGAACGGAA TGACGCCAAG 
AATGCTGTGG AAGAGTATGT GTATGAGATG AGAGACAAGC TTAGTGGTGA ATATGAGAAG 
TTTGTGAGTG AAGATGATCG TAATACTTTT ACCTTAAAAT TAGAGGATAC TGAAAATTGG 
CTGTATGAAG ATGGAGAAGA TCAACCAAAG CAAGTTTATG TAGACAAATT GGCTGAATTA 
AAAAGTTTAG GTCAACCCAT TAAGACACGG TTCCAGGAAT CTGAAGAAAG ACCAAAATTA 
TTTGAAGAAC TAGGGAAGCA AATCCAACAG TATATGAAAG TGATCAGCTC TTTCAAAAAC 
AAGGAGGACC AGTATGAACA CTTGGATGCT GCTGACGTGA CAAAGGTAGA GAAAAGCACC 
AATGAGGCGA TGGAGTGGAT GAATAGCAAG CTTAACCTGC AGAACAAACA GAGCCTGACG 
GTGGATCCAG TTGTCAAGAC AAAGGAGATT GAAGCTAAAA TTAAGGAGCT GACAAGTATT 
TGCAGTCCTA TAATTTCAAA ACCCAAACCC AAAGTGGAAC CCCCAAAGGA GGAACCAAAA 
CATGCTGAGC AGAATGGACC AGTGGATGGC CAAGGAGACA ACCCAGGCTC CCAGGCTGCT 
GAGCACGGTG CAGACACAGC TGTGCCTTCT GATGGCGACA AGAAGCTTCC TGAGATGGAC 
ATTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Geranylgeranylacetone Suppresses N-Methyl-N-nitrosourea-Induced Photoreceptor Cell Loss in Mice.
Advances in experimental medicine and biology854237-43(2016)
Koriyama Y,Ogai K,Sugitani K,Hisano S,Kato S

Heat shock protein A4 controls cell migration and gastric ulcer healing.
Digestive diseases and sciences60(4)850-7(2015 Apr)
Sakurai T,Kashida H,Hagiwara S,Nishida N,Watanabe T,Fujita J,Kudo M

Involvement of heat shock protein a4/apg-2 in refractory inflammatory bowel disease.
Inflammatory bowel diseases21(1)31-9(2015 Jan)
Adachi T,Sakurai T,Kashida H,Mine H,Hagiwara S,Matsui S,Yoshida K,Nishida N,Watanabe T,Itoh K,Fujita J,Kudo M

Therapeutic inducers of the HSP70/HSP110 protect mice against traumatic brain injury.
Journal of neurochemistry130(5)626-41(2014 Sep)
Eroglu B,Kimbler DE,Pang J,Choi J,Moskophidis D,Yanasak N,Dhandapani KM,Mivechi NF

Assessment of the risk of blastomere biopsy during preimplantation genetic diagnosis in a mouse model: reducing female ovary function with an increase in age by proteomics method.
Journal of proteome research12(12)5475-86(2013 Dec)
Yu Y,Zhao Y,Li R,Li L,Zhao H,Li M,Sha J,Zhou Q,Qiao J

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Hspa4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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