Psmc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psmc1
Entrez Gene ID	19179
Full Name	protease (prosome, macropain) 26S subunit, ATPase 1
Synonyms	AI325227,P26s4,Rpt2/S4,S4,rpt2
General protein information	Preferred Names protease (prosome, macropain) 26S subunit, ATPase 1 Names 26S proteasome regulatory subunit 4 26S protease regulatory subunit 4 26S proteasome AAA-ATPase subunit RPT2 proteasome 26S subunit ATPase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 E\|12 50.43 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008947.3	NP_032973.1	26S proteasome regulatory subunit 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PSMC1	XP_510114.2
Caenorhabditis elegans (roundworm)	rpt-2	NP_504558.1
Arabidopsis thaliana (thale cress)	AT2G20140	NP_179604.1
Arabidopsis thaliana (thale cress)	RPT2a	NP_194633.1
Oryza sativa (rice)	Os07g0691800	NP_001060719.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003216	XP_312924.4
Oryza sativa (rice)	Os03g0298400	NP_001049843.1
Danio rerio (zebrafish)	psmc1a	NP_956327.1
Saccharomyces cerevisiae (baker's yeast)	RPT2	NP_010277.1
Eremothecium gossypii	AGOS_AEL011W	NP_984850.1
Homo sapiens (human)	PSMC1	NP_002793.2
	PSMC1	XP_001086338.2
Mus musculus (house mouse)	Psmc1	NP_032973.1
Rattus norvegicus (Norway rat)	Psmc1	NP_476464.1
Danio rerio (zebrafish)	psmc1b	NP_001002091.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003215	XP_312923.4
Kluyveromyces lactis	KLLA0F14707g	XP_455741.1
Magnaporthe oryzae (rice blast fungus)	MGG_05477	XP_003710342.1
Canis lupus familiaris (dog)	PSMC1	NP_001238880.1
Bos taurus (cattle)	PSMC1	NP_001014855.1
Gallus gallus (chicken)	PSMC1	NP_990289.1
Drosophila melanogaster (fruit fly)	Pros26.4	NP_524469.2
Schizosaccharomyces pombe (fission yeast)	rpt2	NP_595480.1
Neurospora crassa	NCU11271	XP_961590.2
Xenopus tropicalis (tropical clawed frog)	psmc1	NP_001039154.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteasome dysfunction activates autophagy and the Keap1-Nrf2 pathway.
Kageyama S, Sou YS, Uemura T, Kametaka S, Saito T, Ishimura R, Kouno T, Bedford L, Mayer RJ, Lee MS, Yamamoto M, Waguri S, Tanaka K, Komatsu M
The Journal of biological chemistry289(36)24944-55(2014 Sep)

Proteasome function is required for platelet production.
Shi DS, Smith MC, Campbell RA, Zimmerman PW, Franks ZB, Kraemer BF, Machlus KR, Ling J, Kamba P, Schwertz H, Rowley JW, Miles RR, Liu ZJ, Sola-Visner M, Italiano JE Jr, Christensen H, Kahr WH, Li DY, Weyrich AS
The Journal of clinical investigation124(9)3757-66(2014 Sep)

Implications for oxidative stress and astrocytes following 26S proteasomal depletion in mouse forebrain neurones.
Elkharaz J, Ugun-Klusek A, Constantin-Teodosiu D, Lawler K, Mayer RJ, Billett E, Lowe J, Bedford L
Biochimica et biophysica acta1832(12)1930-8(2013 Dec)

Phosphorylation of p62 activates the Keap1-Nrf2 pathway during selective autophagy.
Ichimura Y, Waguri S, Sou YS, Kageyama S, Hasegawa J, Ishimura R, Saito T, Yang Y, Kouno T, Fukutomi T, Hoshii T, Hirao A, Takagi K, Mizushima T, Motohashi H, Lee MS, Yoshimori T, Tanaka K, Yamamoto M, Komatsu M
Molecular cell51(5)618-31(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Psmc1(+/-)primary mouse embryonic fibroblasts we found decreased PSMC1 protein expression, altered assembly of the 26S proteasome and G2/M cell cycle arrest.
Title: Heterozygosity for the proteasomal Psmc1 ATPase is insufficient to cause neuropathology in mouse brain, but causes cell cycle defects in mouse embryonic fibroblasts.

Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity
Title: Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity.

The following Psmc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psmc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17455	NM_008947.3 Latest version!	Mus musculus protease (prosome, macropain) 26S subunit, ATPase 1 (Psmc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psmc1 ( NM_008947.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032973.1 Mus musculus protease (prosome, macropain) 26S subunit, ATPase 1 (Psmc1), mRNA.

Price & Availability↑

CloneID	OMu17455
Clone ID Related Accession (Same CDS sequence)	NM_008947.3
Accession Version	NM_008947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	26S proteasome regulatory subunit 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC159243.2 and AC166349.4. On Apr 27, 2007 this sequence version replaced NM_008947.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK148906.1, AK161641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCAAA GCCAGAGTGG TGGCCATGGT CCTGGGGGTG GCAAGAAGGA TGACAAGGAC 
AAGAAAAAGA AATACGAACC TCCTGTCCCA ACTAGAGTGG GGAAAAAGAA GAAGAAAACA 
AAGGGACCAG ATGCTGCCAG CAAACTGCCA CTGGTAACAC CTCACACCCA GTGCCGCCTG 
AAATTACTGA AGCTAGAGAG AATAAAAGAC TATCTTCTCA TGGAGGAGGA ATTCATTAGA 
AACCAGGAAC AGATGAAACC ATTAGAAGAA AAGCAAGAGG AGGAAAGATC AAAAGTGGAT 
GATCTCAGGG GGACCCCCAT GTCTGTAGGA ACCTTGGAAG AGATCATTGA TGATAATCAC 
GCCATTGTGT CCACATCTGT GGGCTCAGAA CACTACGTCA GCATCCTGTC GTTTGTAGAC 
AAGGATCTGC TGGAACCAGG CTGCTCCGTC CTGCTCAACC ACAAGGTGCA TGCTGTGATA 
GGGGTGCTAA TGGATGACAC GGATCCCCTG GTCACAGTGA TGAAGGTGGA AAAGGCCCCC 
CAGGAGACCT ACGCAGATAT TGGGGGACTG GACAACCAGA TCCAAGAAAT TAAGGAGTCT 
GTGGAGCTCC CGCTTACCCA TCCTGAGTAT TACGAGGAGA TGGGGATAAA GCCCCCTAAG 
GGGGTCATTC TCTACGGTCC GCCAGGAACA GGTAAAACTC TATTGGCCAA AGCAGTAGCG 
AACCAGACTT CAGCCACTTT CTTGCGAGTG GTTGGCTCAG AGCTTATCCA GAAGTACCTA 
GGTGACGGGC CCAAACTTGT CCGGGAGCTC TTCCGGGTCG CTGAGGAGCA TGCGCCGTCC 
ATCGTGTTCA TTGATGAGAT CGACGCCATT GGGACCAAAA GATATGATTC AAATTCTGGA 
GGTGAGAGAG AAATTCAGCG AACAATGTTG GAACTGTTGA ACCAGTTGGA TGGATTTGAT 
TCGAGGGGAG ATGTAAAAGT TATCATGGCC ACAAACCGAA TAGAAACTTT GGATCCAGCA 
CTTATCAGGC CAGGCCGCAT TGACAGAAAG ATCGAGTTCC CCCTGCCGGA TGAGAAGACC 
AAGAAGCGTA TCTTCCAGAT TCACACCAGC AGGATGACAC TGGCTGATGA TGTAACCTTG 
GATGACTTGA TCATGGCAAA GGATGACCTC TCTGGGGCCG ACATCAAGGC AATCTGTACA 
GAAGCTGGCT TGATGGCCTT GCGGGAACGC AGAATGAAAG TAACAAATGA AGACTTCAAA 
AAATCTAAAG AGAATGTTCT TTATAAAAAA CAAGAAGGCA CCCCTGAGGG GCTCTATCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032973.1
CDS	8..1330
Translation	MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

Target ORF information:

RefSeq Version	NM_008947.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease (prosome, macropain) 26S subunit, ATPase 1 (Psmc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008947.3

ORF Insert Sequence:

ATGGGTCAAA GCCAGAGTGG TGGCCATGGT CCTGGGGGTG GCAAGAAGGA TGACAAGGAC 
AAGAAAAAGA AATACGAACC TCCTGTCCCA ACTAGAGTGG GGAAAAAGAA GAAGAAAACA 
AAGGGACCAG ATGCTGCCAG CAAACTGCCA CTGGTAACAC CTCACACCCA GTGCCGCCTG 
AAATTACTGA AGCTAGAGAG AATAAAAGAC TATCTTCTCA TGGAGGAGGA ATTCATTAGA 
AACCAGGAAC AGATGAAACC ATTAGAAGAA AAGCAAGAGG AGGAAAGATC AAAAGTGGAT 
GATCTCAGGG GGACCCCCAT GTCTGTAGGA ACCTTGGAAG AGATCATTGA TGATAATCAC 
GCCATTGTGT CCACATCTGT GGGCTCAGAA CACTACGTCA GCATCCTGTC GTTTGTAGAC 
AAGGATCTGC TGGAACCAGG CTGCTCCGTC CTGCTCAACC ACAAGGTGCA TGCTGTGATA 
GGGGTGCTAA TGGATGACAC GGATCCCCTG GTCACAGTGA TGAAGGTGGA AAAGGCCCCC 
CAGGAGACCT ACGCAGATAT TGGGGGACTG GACAACCAGA TCCAAGAAAT TAAGGAGTCT 
GTGGAGCTCC CGCTTACCCA TCCTGAGTAT TACGAGGAGA TGGGGATAAA GCCCCCTAAG 
GGGGTCATTC TCTACGGTCC GCCAGGAACA GGTAAAACTC TATTGGCCAA AGCAGTAGCG 
AACCAGACTT CAGCCACTTT CTTGCGAGTG GTTGGCTCAG AGCTTATCCA GAAGTACCTA 
GGTGACGGGC CCAAACTTGT CCGGGAGCTC TTCCGGGTCG CTGAGGAGCA TGCGCCGTCC 
ATCGTGTTCA TTGATGAGAT CGACGCCATT GGGACCAAAA GATATGATTC AAATTCTGGA 
GGTGAGAGAG AAATTCAGCG AACAATGTTG GAACTGTTGA ACCAGTTGGA TGGATTTGAT 
TCGAGGGGAG ATGTAAAAGT TATCATGGCC ACAAACCGAA TAGAAACTTT GGATCCAGCA 
CTTATCAGGC CAGGCCGCAT TGACAGAAAG ATCGAGTTCC CCCTGCCGGA TGAGAAGACC 
AAGAAGCGTA TCTTCCAGAT TCACACCAGC AGGATGACAC TGGCTGATGA TGTAACCTTG 
GATGACTTGA TCATGGCAAA GGATGACCTC TCTGGGGCCG ACATCAAGGC AATCTGTACA 
GAAGCTGGCT TGATGGCCTT GCGGGAACGC AGAATGAAAG TAACAAATGA AGACTTCAAA 
AAATCTAAAG AGAATGTTCT TTATAAAAAA CAAGAAGGCA CCCCTGAGGG GCTCTATCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteasome dysfunction activates autophagy and the Keap1-Nrf2 pathway.
The Journal of biological chemistry289(36)24944-55(2014 Sep)
Kageyama S,Sou YS,Uemura T,Kametaka S,Saito T,Ishimura R,Kouno T,Bedford L,Mayer RJ,Lee MS,Yamamoto M,Waguri S,Tanaka K,Komatsu M

Proteasome function is required for platelet production.
The Journal of clinical investigation124(9)3757-66(2014 Sep)
Shi DS,Smith MC,Campbell RA,Zimmerman PW,Franks ZB,Kraemer BF,Machlus KR,Ling J,Kamba P,Schwertz H,Rowley JW,Miles RR,Liu ZJ,Sola-Visner M,Italiano JE Jr,Christensen H,Kahr WH,Li DY,Weyrich AS

Implications for oxidative stress and astrocytes following 26S proteasomal depletion in mouse forebrain neurones.
Biochimica et biophysica acta1832(12)1930-8(2013 Dec)
Elkharaz J,Ugun-Klusek A,Constantin-Teodosiu D,Lawler K,Mayer RJ,Billett E,Lowe J,Bedford L

Phosphorylation of p62 activates the Keap1-Nrf2 pathway during selective autophagy.
Molecular cell51(5)618-31(2013 Sep)
Ichimura Y,Waguri S,Sou YS,Kageyama S,Hasegawa J,Ishimura R,Saito T,Yang Y,Kouno T,Fukutomi T,Hoshii T,Hirao A,Takagi K,Mizushima T,Motohashi H,Lee MS,Yoshimori T,Tanaka K,Yamamoto M,Komatsu M

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Psmc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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