Robo1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Robo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Robo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu52700 XM_006522960.4
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52701 XM_006522961.3
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52702 XM_006522962.4
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52703 XM_006522963.4
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52704 XM_006522964.4
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52705 XM_006522965.2
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu79277 XM_017316893.1
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu79278 XM_017316894.2
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu86897 XM_030249003.1
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu86898 XM_030249005.1
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu22089 NM_019413.2
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu52697 XM_006522957.2
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu52698 XM_006522958.2
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu68623 XM_011246105.2
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu68624 XM_011246106.2
Latest version!
Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OMu52700 XM_006522960.3 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu79278 XM_017316894.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu52702 XM_006522962.3 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52704 XM_006522964.3 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52701 XM_006522961.2 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52703 XM_006522963.3 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu52705 XM_006522965.1 Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu52700
    Clone ID Related Accession (Same CDS sequence) XM_006522960.3 , XM_006522960.4
    Accession Version XM_006522960.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG
    GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC
    ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC
    CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC
    CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC
    TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG
    AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT
    GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT
    TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC
    CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA
    AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC
    AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA
    ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT
    GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG
    AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA
    ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC
    GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC
    GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA
    GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT
    TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC
    ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT
    GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT
    CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC
    CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC
    CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC
    TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG
    GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA
    CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC
    AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT
    TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT
    GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG
    GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA
    ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG
    GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG
    CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA
    TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA
    AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA
    TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA
    GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA
    ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT
    AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG
    ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA
    GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT
    CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG
    GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC
    TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG
    GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT
    GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT
    GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC
    TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT
    CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA
    GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT
    CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG
    GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG
    TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC
    ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC
    ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA
    GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG
    ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC
    GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT
    GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT
    GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT
    GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG
    GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT
    ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA
    GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA
    CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA
    CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA
    ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG
    AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523023.1
    CDS426..5402
    Translation

    Target ORF information:

    RefSeq Version XM_006522960.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522960.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG
    GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC
    ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC
    CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC
    CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC
    TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG
    AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT
    GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT
    TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC
    CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA
    AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC
    AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA
    ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT
    GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG
    AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA
    ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC
    GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC
    GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA
    GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT
    TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC
    ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT
    GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT
    CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC
    CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC
    CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC
    TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG
    GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA
    CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC
    AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT
    TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT
    GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG
    GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA
    ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG
    GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG
    CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA
    TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA
    AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA
    TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA
    GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA
    ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT
    AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG
    ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA
    GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT
    CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG
    GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC
    TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG
    GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT
    GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT
    GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC
    TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT
    CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA
    GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT
    CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG
    GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG
    TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC
    ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC
    ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA
    GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG
    ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC
    GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT
    GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT
    GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT
    GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG
    GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT
    ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA
    GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA
    CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA
    CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA
    ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG
    AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52701
    Clone ID Related Accession (Same CDS sequence) XM_006522961.2 , XM_006522961.3
    Accession Version XM_006522961.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA
    GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG
    TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG
    CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC
    GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA
    TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG
    GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC
    ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA
    GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT
    GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA
    CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT
    AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG
    CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT
    GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT
    TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG
    GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT
    CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT
    CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC
    CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC
    ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC
    ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT
    GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT
    TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC
    TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT
    TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA
    TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT
    GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA
    CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA
    CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC
    CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT
    GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC
    CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT
    CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT
    CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG
    CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC
    GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC
    ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG
    GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG
    TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG
    TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT
    TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC
    CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT
    GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC
    ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC
    AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC
    ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC
    TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG
    CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC
    AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT
    ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT
    GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC
    AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG
    CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC
    CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA
    TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT
    CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT
    CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT
    GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT
    GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT
    GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG
    GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC
    ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG
    GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA
    AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC
    AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT
    GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG
    GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA
    GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA
    CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT
    GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC
    CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT
    AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG
    CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA
    GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523024.1
    CDS471..5372
    Translation

    Target ORF information:

    RefSeq Version XM_006522961.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522961.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA
    GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG
    TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG
    CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC
    GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA
    TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG
    GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC
    ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA
    GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT
    GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA
    CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT
    AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG
    CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT
    GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT
    TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG
    GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT
    CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT
    CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC
    CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC
    ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC
    ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT
    GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT
    TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC
    TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT
    TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA
    TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT
    GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA
    CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA
    CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC
    CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT
    GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC
    CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT
    CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT
    CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG
    CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC
    GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC
    ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG
    GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG
    TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG
    TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT
    TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC
    CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT
    GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC
    ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC
    AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC
    ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC
    TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG
    CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC
    AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT
    ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT
    GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC
    AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG
    CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC
    CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA
    TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT
    CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT
    CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT
    GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT
    GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT
    GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG
    GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC
    ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG
    GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA
    AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC
    AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT
    GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG
    GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA
    GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA
    CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT
    GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC
    CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT
    AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG
    CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA
    GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52702
    Clone ID Related Accession (Same CDS sequence) XM_006522962.4 , XM_006522962.3
    Accession Version XM_006522962.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522962.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC
    AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC
    AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC
    CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG
    AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC
    CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA
    TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT
    GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA
    CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC
    ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC
    ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG
    GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT
    CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC
    AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT
    GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT
    CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG
    CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA
    CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA
    CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT
    AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA
    GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC
    GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC
    GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA
    GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA
    GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523025.1
    CDS98..4996
    Translation

    Target ORF information:

    RefSeq Version XM_006522962.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522962.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC
    AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC
    AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC
    CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG
    AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC
    CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA
    TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT
    GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA
    CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC
    ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC
    ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG
    GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT
    CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC
    AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT
    GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT
    CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG
    CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA
    CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA
    CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT
    AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA
    GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC
    GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC
    GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA
    GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA
    GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52703
    Clone ID Related Accession (Same CDS sequence) XM_006522963.3 , XM_006522963.4
    Accession Version XM_006522963.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522963.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA
    GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT
    GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT
    GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC
    GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC
    TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG
    GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC
    GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG
    AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA
    CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC
    ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT
    GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG
    GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA
    TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC
    ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT
    TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG
    GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT
    ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC
    TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT
    GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC
    TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT
    GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT
    CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG
    ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT
    CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA
    GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC
    AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG
    CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT
    GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC
    AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA
    ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC
    CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC
    AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT
    CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG
    GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC
    AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA
    GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT
    GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT
    CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA
    ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT
    CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG
    GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG
    GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA
    GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC
    CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT
    ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG
    CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC
    AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC
    AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC
    CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG
    GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG
    CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG
    AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT
    CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG
    CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC
    TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG
    AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC
    TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA
    TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG
    CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC
    CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC
    ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC
    CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG
    CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT
    ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG
    CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC
    CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC
    AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT
    GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC
    CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG
    AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC
    AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA
    CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG
    GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG
    TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT
    GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA
    GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA
    GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC
    AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT
    GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523026.1
    CDS426..5318
    Translation

    Target ORF information:

    RefSeq Version XM_006522963.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522963.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA
    GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT
    GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT
    GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC
    GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC
    TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG
    GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC
    GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG
    AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA
    CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC
    ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT
    GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG
    GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA
    TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC
    ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT
    TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG
    GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT
    ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC
    TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT
    GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC
    TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT
    GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT
    CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG
    ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT
    CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA
    GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC
    AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG
    CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT
    GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC
    AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA
    ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC
    CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC
    AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT
    CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG
    GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC
    AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA
    GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT
    GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT
    CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA
    ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT
    CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG
    GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG
    GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA
    GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC
    CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT
    ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG
    CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC
    AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC
    AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC
    CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG
    GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG
    CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG
    AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT
    CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG
    CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC
    TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG
    AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC
    TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA
    TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG
    CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC
    CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC
    ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC
    CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG
    CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT
    ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG
    CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC
    CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC
    AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT
    GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC
    CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG
    AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC
    AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA
    CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG
    GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG
    TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT
    GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA
    GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA
    GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC
    AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT
    GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52704
    Clone ID Related Accession (Same CDS sequence) XM_006522964.4 , XM_006522964.3
    Accession Version XM_006522964.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522964.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG
    CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT
    AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA
    GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA
    AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG
    TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG
    GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT
    CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA
    AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT
    GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT
    TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA
    CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT
    CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA
    GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA
    CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC
    AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT
    GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC
    CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC
    AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA
    GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT
    GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG
    CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT
    TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT
    GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC
    AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT
    AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA
    TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA
    GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523027.1
    CDS99..4970
    Translation

    Target ORF information:

    RefSeq Version XM_006522964.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522964.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG
    CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT
    AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA
    GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA
    AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG
    TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG
    GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT
    CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA
    AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT
    GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT
    TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA
    CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT
    CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA
    GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA
    CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC
    AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT
    GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC
    CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC
    AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA
    GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT
    GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG
    CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT
    TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT
    GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC
    AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT
    AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA
    TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA
    GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52705
    Clone ID Related Accession (Same CDS sequence) XM_006522965.1 , XM_006522965.2
    Accession Version XM_006522965.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA
    CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC
    CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG
    AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT
    ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT
    GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC
    CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG
    GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA
    GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT
    GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT
    GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC
    AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG
    GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT
    GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT
    GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG
    CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG
    AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG
    GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG
    GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA
    GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT
    CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG
    AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC
    CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC
    AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT
    AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT
    CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA
    AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC
    CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG
    GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG
    AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG
    GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA
    TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG
    AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG
    TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG
    AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC
    GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG
    TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA
    AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT
    GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG
    CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG
    ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC
    AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA
    AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523028.1
    CDS1366..6213
    Translation

    Target ORF information:

    RefSeq Version XM_006522965.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522965.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA
    CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC
    CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG
    AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT
    ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT
    GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC
    CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG
    GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA
    GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT
    GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT
    GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC
    AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG
    GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT
    GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT
    GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG
    CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG
    AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG
    GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG
    GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA
    GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT
    CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG
    AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC
    CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC
    AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT
    AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT
    CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA
    AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC
    CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG
    GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG
    AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG
    GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA
    TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG
    AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG
    TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG
    AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC
    GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG
    TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA
    AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT
    GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG
    CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG
    ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC
    AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA
    AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79277
    Clone ID Related Accession (Same CDS sequence) XM_017316893.1
    Accession Version XM_017316893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC
    AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC
    AACCTGACAA CCTACAGTCG CCCAGCTGAT TGTATAGCCA ATTATAACAA CCAACTGGAT
    AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT
    AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGACGG GCGTTTTGTC
    AATCCATCAG GGCAGCCCAC TCCCTATGCC ACCACTCAAC TCATCCAGGC GAACCTCAGC
    AACAATATGA ATAATGGCGC CGGGGACTCC AGCGAGAAGC ACTGGAAGCC TCCGGGACAG
    CAGAAACCGG AAGTGGCACC AATTCAGTAT AACATCATGG AGCAAAACAA GCTGAACAAA
    GATTATCGGG CAAATGACAC GATCCCTCCA ACCATCCCAT ACAACCAGTC CTATGACCAG
    AACACAGGAG GGTCTTATAA CAGCTCAGAT CGGGGCAGTA GTACATCCGG GAGTCAAGGC
    CACAAGAAAG GGGCACGGAC ACCAAAGGCA CCCAAGCAGG GTGGCATGAA CTGGGCAGAC
    TTGCTGCCCC CTCCTCCGGC GCATCCTCCC CCACATAGCA ACAGTGAAGA ATACAACATG
    TCCGTAGATG AAAGCTATGA TCAAGAAATG CCCTGTCCAG TGCCACCTGC GCGGATGTAT
    TTGCAACAGG ATGAATTAGA GGAAGAGGAA GATGAAAGAG GCCCCACTCC CCCTGTCCGG
    GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC TACAGCCACC AATCCACGGC CACTCTCACC
    CCTTCTCCCC AGGAAGAGCT CCAGCCCATG TTACAGGATT GTCCGGAGGA CCTCGGGCAC
    ATGCCCCACC CACCAGACAG GAGGCGGCAG CCCGTGAGTC CTCCTCCACC GCCACGGCCA
    ATTTCCCCAC CTCATACTTA CGGCTACATT TCAGGACCCC TTGTCTCAGA TATGGATACC
    GATGCCCCAG AAGAGGAGGA GGACGAAGCT GACATGGAAG TTGCCAAAAT GCAGACCCGA
    AGGCTGTTGT TACGTGGGCT GGAGCAGACC CCAGCATCCA GTGTTGGGGA CCTTGAGAGC
    TCCGTCACTG GGTCCATGAT CAATGGCTGG GGCTCAGCCT CGGAAGAGGA CAACATTTCC
    AGTGGGCGCT CCAGTGTCAG TTCATCCGAT GGCTCCTTTT TCACTGACGC TGATTTTGCC
    CAGGCAGTTG CTGCAGCTGC GGAGTATGCG GGCCTGAAAG TGGCTCGCCG CCAAATGCAA
    GATGCTGCTG GCCGCCGCCA CTTCCATGCC TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG
    TCCACAGACA GCAACATGAG TGCTGTTGTG ATCCAGAAAG CCAGACCCGC CAAGAAGCAG
    AAACACCAGC CAGGACATCT GCGCAGGGAA GCCTACGCAG ATGATCTTCC ACCCCCTCCA
    GTGCCACCAC CTGCTATAAA ATCGCCCACT GTCCAGTCCA AGGCACAGCT GGAGGTACGG
    CCTGTCATGG TGCCAAAACT CGCGTCTATA GAAGCAAGGA CAGATAGATC GTCAGACAGA
    AAAGGAGGCA GTTACAAGGG GAGAGAAGCT CTGGATGGAA GACAAGTCAC TGACCTGCGA
    ACAAATCCAA GTGACCCCAG AGAGGCACAG GAACAGCCAA ATGACGGGAA AGGCCGAGGA
    ACAAAGGCAG CCAAGCGAGA CCTTCCACCA GCAAAGACGC ACCTCGGCCA AGAGGATATT
    CTTCCGTACT GTAGACCTAC TTTCCCAACG TCAAACAACC CTAGAGACCC CAGTTCCTCC
    AGCTCTATGT CATCAAGAGG ATCAGGGAGC CGACAAAGAG AGCAAGCAAA TGTGGGCCGA
    AGGAATATGG CAGAAATGCA AGTACTTGGG GGATTTGAAA GAGGAGATGA GAATAATGAA
    GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172382.1
    CDS98..5161
    Translation

    Target ORF information:

    RefSeq Version XM_017316893.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017316893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC
    AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC
    AACCTGACAA CCTACAGTCG CCCAGCTGAT TGTATAGCCA ATTATAACAA CCAACTGGAT
    AACAAACAAA CAAATCTGAT GCTCCCTGAG TCAACTGTTT ATGGTGATGT GGACCTTAGT
    AACAAAATCA ATGAGATGAA AACCTTCAAT AGCCCAAATC TGAAGGACGG GCGTTTTGTC
    AATCCATCAG GGCAGCCCAC TCCCTATGCC ACCACTCAAC TCATCCAGGC GAACCTCAGC
    AACAATATGA ATAATGGCGC CGGGGACTCC AGCGAGAAGC ACTGGAAGCC TCCGGGACAG
    CAGAAACCGG AAGTGGCACC AATTCAGTAT AACATCATGG AGCAAAACAA GCTGAACAAA
    GATTATCGGG CAAATGACAC GATCCCTCCA ACCATCCCAT ACAACCAGTC CTATGACCAG
    AACACAGGAG GGTCTTATAA CAGCTCAGAT CGGGGCAGTA GTACATCCGG GAGTCAAGGC
    CACAAGAAAG GGGCACGGAC ACCAAAGGCA CCCAAGCAGG GTGGCATGAA CTGGGCAGAC
    TTGCTGCCCC CTCCTCCGGC GCATCCTCCC CCACATAGCA ACAGTGAAGA ATACAACATG
    TCCGTAGATG AAAGCTATGA TCAAGAAATG CCCTGTCCAG TGCCACCTGC GCGGATGTAT
    TTGCAACAGG ATGAATTAGA GGAAGAGGAA GATGAAAGAG GCCCCACTCC CCCTGTCCGG
    GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC TACAGCCACC AATCCACGGC CACTCTCACC
    CCTTCTCCCC AGGAAGAGCT CCAGCCCATG TTACAGGATT GTCCGGAGGA CCTCGGGCAC
    ATGCCCCACC CACCAGACAG GAGGCGGCAG CCCGTGAGTC CTCCTCCACC GCCACGGCCA
    ATTTCCCCAC CTCATACTTA CGGCTACATT TCAGGACCCC TTGTCTCAGA TATGGATACC
    GATGCCCCAG AAGAGGAGGA GGACGAAGCT GACATGGAAG TTGCCAAAAT GCAGACCCGA
    AGGCTGTTGT TACGTGGGCT GGAGCAGACC CCAGCATCCA GTGTTGGGGA CCTTGAGAGC
    TCCGTCACTG GGTCCATGAT CAATGGCTGG GGCTCAGCCT CGGAAGAGGA CAACATTTCC
    AGTGGGCGCT CCAGTGTCAG TTCATCCGAT GGCTCCTTTT TCACTGACGC TGATTTTGCC
    CAGGCAGTTG CTGCAGCTGC GGAGTATGCG GGCCTGAAAG TGGCTCGCCG CCAAATGCAA
    GATGCTGCTG GCCGCCGCCA CTTCCATGCC TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG
    TCCACAGACA GCAACATGAG TGCTGTTGTG ATCCAGAAAG CCAGACCCGC CAAGAAGCAG
    AAACACCAGC CAGGACATCT GCGCAGGGAA GCCTACGCAG ATGATCTTCC ACCCCCTCCA
    GTGCCACCAC CTGCTATAAA ATCGCCCACT GTCCAGTCCA AGGCACAGCT GGAGGTACGG
    CCTGTCATGG TGCCAAAACT CGCGTCTATA GAAGCAAGGA CAGATAGATC GTCAGACAGA
    AAAGGAGGCA GTTACAAGGG GAGAGAAGCT CTGGATGGAA GACAAGTCAC TGACCTGCGA
    ACAAATCCAA GTGACCCCAG AGAGGCACAG GAACAGCCAA ATGACGGGAA AGGCCGAGGA
    ACAAAGGCAG CCAAGCGAGA CCTTCCACCA GCAAAGACGC ACCTCGGCCA AGAGGATATT
    CTTCCGTACT GTAGACCTAC TTTCCCAACG TCAAACAACC CTAGAGACCC CAGTTCCTCC
    AGCTCTATGT CATCAAGAGG ATCAGGGAGC CGACAAAGAG AGCAAGCAAA TGTGGGCCGA
    AGGAATATGG CAGAAATGCA AGTACTTGGG GGATTTGAAA GAGGAGATGA GAATAATGAA
    GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79278
    Clone ID Related Accession (Same CDS sequence) XM_017316894.1 , XM_017316894.2
    Accession Version XM_017316894.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC
    CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG
    CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC
    CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC
    CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT
    GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC
    CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC
    GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA
    CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT
    CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT
    AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC
    GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC
    GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG
    ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT
    CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT
    ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA
    AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG
    CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT
    CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG
    AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA
    GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT
    TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA
    GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC
    ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG
    GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG
    CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC
    AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA
    CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA
    GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC
    CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG
    GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC
    CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT
    CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA
    AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA
    GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC
    TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG
    CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG
    GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC
    TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT
    GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC
    CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC
    GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG
    GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG
    TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC
    TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC
    CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC
    ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA
    AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT
    GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC
    TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT
    CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG
    ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG
    AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC
    ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC
    GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA
    CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC
    ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT
    AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG
    ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG
    GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT
    GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA
    GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA
    GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG
    CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC
    AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT
    TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG
    GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG
    CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG
    ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC
    AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT
    GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC
    CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG
    AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT
    CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA
    AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA
    CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG
    GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC
    AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA
    GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT
    ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA
    GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG
    CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172383.1
    CDS124..5109
    Translation

    Target ORF information:

    RefSeq Version XM_017316894.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017316894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC
    CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG
    CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC
    CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC
    CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT
    GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC
    CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC
    GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA
    CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT
    CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT
    AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC
    GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC
    GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG
    ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT
    CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT
    ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA
    AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG
    CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT
    CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG
    AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA
    GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT
    TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA
    GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC
    ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG
    GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG
    CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC
    AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA
    CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA
    GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC
    CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG
    GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC
    CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT
    CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA
    AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA
    GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC
    TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG
    CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG
    GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC
    TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT
    GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC
    CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC
    GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG
    GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG
    TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC
    TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC
    CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC
    ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA
    AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT
    GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC
    TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT
    CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG
    ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG
    AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC
    ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC
    GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA
    CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC
    ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT
    AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG
    ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG
    GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT
    GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA
    GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA
    GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG
    CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC
    AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT
    TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG
    GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG
    CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG
    ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC
    AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT
    GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC
    CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG
    AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT
    CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA
    AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA
    CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG
    GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC
    AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA
    GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT
    ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA
    GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG
    CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu79278
    Clone ID Related Accession (Same CDS sequence) XM_017316894.1 , XM_017316894.2
    Accession Version XM_017316894.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC
    CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG
    CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC
    CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC
    CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT
    GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC
    CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC
    GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA
    CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT
    CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT
    AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC
    GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC
    GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG
    ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT
    CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT
    ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA
    AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG
    CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT
    CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG
    AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA
    GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT
    TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA
    GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC
    ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG
    GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG
    CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC
    AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA
    CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA
    GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC
    CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG
    GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC
    CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT
    CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA
    AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA
    GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC
    TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG
    CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG
    GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC
    TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT
    GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC
    CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC
    GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG
    GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG
    TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC
    TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC
    CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC
    ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA
    AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT
    GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC
    TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT
    CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG
    ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG
    AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC
    ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC
    GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA
    CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC
    ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT
    AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG
    ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG
    GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT
    GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA
    GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA
    GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG
    CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC
    AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT
    TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG
    GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG
    CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG
    ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC
    AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT
    GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC
    CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG
    AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT
    CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA
    AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA
    CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG
    GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC
    AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA
    GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT
    ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA
    GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG
    CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172383.1
    CDS473..5458
    Translation

    Target ORF information:

    RefSeq Version XM_017316894.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017316894.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCA CCCTGAGGAC
    CTAGAGAGGG GGAACGACAA CGGAACGCCA GCCCCCACCT CTGATAACGA TGACAATTCG
    CTGGGCTACA CAGGCTCCCG TCTTCGTCAG GAAGATTTTC CACCCCGCAT TGTTGAACAC
    CCTTCAGACC TGATCGTCTC CAAAGGAGAA CCTGCCACTC TGAACTGTAA AGCCGAAGGC
    CGCCCCACCC CGACGATAGA ATGGTACAAA GGTGGGGAAA GAGTGGAGAC GGACAAAGAT
    GACCCGCGCT CACACCGGAT GCTGCTGCCA AGCGGGTCTT TATTTTTCTT ACGCATAGTC
    CATGGACGGA AGAGTAGGCC CGACGAAGGG GTCTACATCT GTGTGGCTAG GAATTACCTC
    GGAGAGGCTG TGAGCCACAA CGCATCGCTG GAGGTAGCTA TACTACGGGA TGACTTCAGA
    CAAAACCCTT CAGATGTCAT GGTGGCAGTA GGCGAACCTG CAGTCATGGA ATGCCAGCCT
    CCACGGGGCC ATCCAGAGCC CACCATCTCC TGGAAAAAGG ATGGCTCACC CCTGGATGAT
    AAAGATGAAA GAATCACTAT ACGAGGAGGA AAACTCATGA TCACTTATAC GCGTAAAAGC
    GATGCTGGCA AGTATGTTTG CGTTGGCACC AACATGGTGG GGGAACGGGA GAGTGAAGTC
    GCAGAGCTAA CCGTTTTAGA GAGACCATCG TTTGTGAAGA GACCCAGTAA TTTGGCAGTG
    ACTGTGGATG ACAGTGCAGA ATTTAAATGT GAGGCCCGAG GAGACCCTGT GCCTACAGTT
    CGATGGAGGA AAGATGACGG AGAGCTGCCC AAATCCAGAT ATGAAATCCG AGATGATCAT
    ACTTTGAAAA TTCGGAAGGT GACTGCTGGC GACATGGGAT CATACACTTG TGTGGCAGAA
    AATATGGTCG GGAAAGCTGA AGCTTCCGCT ACTTTGACAG TTCAAGTTGG GTCTGAGCCG
    CCACATTTCG TTGTAAAACC TCGGGACCAG GTGGTTGCCT TGGGAAGAAC TGTGACGTTT
    CAGTGCGAAG CAACTGGAAA TCCTCAACCA GCTATCTTTT GGAGGAGAGA GGGGAGTCAG
    AATCTACTTT TCTCCTACCA GCCTCCACAG TCCTCTAGCC GATTTTCAGT TTCGCAGACA
    GGAGACCTCA CCATTACGAA CGTCCAGCGG TCTGATGTCG GCTACTACAT CTGTCAGACT
    TTAAATGTTG CTGGAAGTAT CATCACAAAG GCCTATTTGG AAGTTACTGA TGTGATTGCA
    GACCGGCCTC CCCCAGTCAT TCGGCAAGGT CCTGTGAATC AGACTGTAGC AGTGGATGGC
    ACTTTAATCC TCAGCTGTGT GGCCACAGGC AGTCCAGCAC CCACAATTCT GTGGAGGAAG
    GATGGAGTCC TTGTTTCAAC CCAAGATTCT CGGATCAAAC AGCTGGAGAG TGGCGTGCTG
    CAGATCCGCT ACGCTAAGCT GGGTGACACG GGTCGGTACA CCTGCACCGC ATCGACCCCC
    AGCGGTGAGG CCACGTGGAG CGCTTACATC GAAGTCCAGG AATTTGGGGT TCCAGTTCAA
    CCCCCAAGAC CCACCGACCC CAACTTAATC CCCAGCGCCC CCTCAAAGCC CGAAGTTACA
    GATGTCAGCA AAAACACGGT CACTTTATCG TGGCAGCCAA ACTTGAACTC AGGAGCAACC
    CCAACATCTT ATATTATAGA AGCCTTCAGC CATGCATCTG GTAGCAGCTG GCAGACGGCG
    GCAGAAAATG TGAAAACAGA AACATTTGCC ATCAAAGGAC TCAAACCTAA CGCCATTTAC
    CTTTTCCTGG TGAGGGCAGC AAATGCCTAT GGAATTAGTG ATCCAAGCCA AATATCAGAT
    CCAGTGAAAA CGCAAGATGT CCCACCAACC AGCCAAGGGG TGGACCACAA GCAGGTCCAA
    AGAGAACTGG GGAATGTTGT CTTACATCTG CACAACCCGA CCATCCTTTC TTCCTCCTCA
    GTCGAAGTGC ACTGGACGGT GGACCAACAA TCTCAATATA TTCAAGGCTA TAAAATTCTC
    TACCGCCCAT CTGGAGCCAG CCATGGTGAA TCGGAGTGGT TAGTTTTTGA AGTGAGGACG
    CCAACGAAAA ACAGCGTGGT GATCCCCGAT CTCAGAAAAG GCGTCAACTA CGAGATTAAG
    GCTCGCCCAT TTTTTAATGA GTTTCAAGGA GCAGACAGTG AAATCAAATT TGCCAAAACC
    TTAGAAGAAG CACCAAGTGC CCCTCCCCGA AGTGTAACTG TATCCAAGAA TGATGGAAAT
    GGGACTGCAA TCCTGGTCAC TTGGCAACCG CCTCCTGAAG ACACACAAAA TGGAATGGTC
    CAAGAATATA AGGTTTGGTG TCTCGGTAAT GAAACGAAGT ATCACATAAA CAAGACAGTC
    GATGGCTCGA CCTTCTCGGT GGTCATCCCC TCTCTGGTTC CTGGGATCCG ATACAGTGTG
    GAGGTGGCAG CGAGCACTGG GGCGGGTCCC GGGGTGAAGA GCGAACCTCA GTTCATCCAG
    TTAGATTCTC ACGGAAACCC CGTGTCTCCC GAGGACCAAG TCAGCCTTGC TCAGCAGATC
    TCCGACGTGG TGAGGCAGCC GGCGTTCATT GCAGGCATTG GGGCAGCCTG TTGGATTATC
    CTCATGGTCT TCAGCATCTG GCTTTACAGA CACCGCAAGA AGAGAAACGG ACTCACCAGC
    ACGTATGCGG GTATCCGGAA AGTCCCGTCC TTTACCTTCA CGCCAACAGT AACTTATCAA
    AGAGGAGGCG AAGCCGTCAG CAGTGGAGGG AGGCCGGGAC TCCTCAACAT TAGTGAACCT
    GCCACGCAGC CATGGCTGGC AGACACGTGG CCCAACACAG GCAACAACCA CAATGACTGC
    TCCATCAACT GCTGCACGGC CGGCAATGGC AACAGCGACA GCAACCTGAC AACCTACAGT
    CGCCCAGCTG ATTGTATAGC CAATTATAAC AACCAACTGG ATAACAAACA AACAAATCTG
    ATGCTCCCTG AGTCAACTGT TTATGGTGAT GTGGACCTTA GTAACAAAAT CAATGAGATG
    AAAACCTTCA ATAGCCCAAA TCTGAAGGAC GGGCGTTTTG TCAATCCATC AGGGCAGCCC
    ACTCCCTATG CCACCACTCA ACTCATCCAG GCGAACCTCA GCAACAATAT GAATAATGGC
    GCCGGGGACT CCAGCGAGAA GCACTGGAAG CCTCCGGGAC AGCAGAAACC GGAAGTGGCA
    CCAATTCAGT ATAACATCAT GGAGCAAAAC AAGCTGAACA AAGATTATCG GGCAAATGAC
    ACGATCCCTC CAACCATCCC ATACAACCAG TCCTATGACC AGAACACAGG AGGGTCTTAT
    AACAGCTCAG ATCGGGGCAG TAGTACATCC GGGAGTCAAG GCCACAAGAA AGGGGCACGG
    ACACCAAAGG CACCCAAGCA GGGTGGCATG AACTGGGCAG ACTTGCTGCC CCCTCCTCCG
    GCGCATCCTC CCCCACATAG CAACAGTGAA GAATACAACA TGTCCGTAGA TGAAAGCTAT
    GATCAAGAAA TGCCCTGTCC AGTGCCACCT GCGCGGATGT ATTTGCAACA GGATGAATTA
    GAGGAAGAGG AAGATGAAAG AGGCCCCACT CCCCCTGTCC GGGGGGCAGC TTCTTCTCCA
    GCTGCTGTGT CCTACAGCCA CCAATCCACG GCCACTCTCA CCCCTTCTCC CCAGGAAGAG
    CTCCAGCCCA TGTTACAGGA TTGTCCGGAG GACCTCGGGC ACATGCCCCA CCCACCAGAC
    AGGAGGCGGC AGCCCGTGAG TCCTCCTCCA CCGCCACGGC CAATTTCCCC ACCTCATACT
    TACGGCTACA TTTCAGGACC CCTTGTCTCA GATATGGATA CCGATGCCCC AGAAGAGGAG
    GAGGACGAAG CTGACATGGA AGTTGCCAAA ATGCAGACCC GAAGGCTGTT GTTACGTGGG
    CTGGAGCAGA CCCCAGCATC CAGTGTTGGG GACCTTGAGA GCTCCGTCAC TGGGTCCATG
    ATCAATGGCT GGGGCTCAGC CTCGGAAGAG GACAACATTT CCAGTGGGCG CTCCAGTGTC
    AGTTCATCCG ATGGCTCCTT TTTCACTGAC GCTGATTTTG CCCAGGCAGT TGCTGCAGCT
    GCGGAGTATG CGGGCCTGAA AGTGGCTCGC CGCCAAATGC AAGATGCTGC TGGCCGCCGC
    CACTTCCATG CCTCTCAGTG CCCAAGGCCC ACGAGTCCTG TGTCCACAGA CAGCAACATG
    AGTGCTGTTG TGATCCAGAA AGCCAGACCC GCCAAGAAGC AGAAACACCA GCCAGGACAT
    CTGCGCAGGG AAGCCTACGC AGATGATCTT CCACCCCCTC CAGTGCCACC ACCTGCTATA
    AAATCGCCCA CTGTCCAGTC CAAGGCACAG CTGGAGGTAC GGCCTGTCAT GGTGCCAAAA
    CTCGCGTCTA TAGAAGCAAG GACAGATAGA TCGTCAGACA GAAAAGGAGG CAGTTACAAG
    GGGAGAGAAG CTCTGGATGG AAGACAAGTC ACTGACCTGC GAACAAATCC AAGTGACCCC
    AGAGAGGCAC AGGAACAGCC AAATGACGGG AAAGGCCGAG GAACAAAGGC AGCCAAGCGA
    GACCTTCCAC CAGCAAAGAC GCACCTCGGC CAAGAGGATA TTCTTCCGTA CTGTAGACCT
    ACTTTCCCAA CGTCAAACAA CCCTAGAGAC CCCAGTTCCT CCAGCTCTAT GTCATCAAGA
    GGATCAGGGA GCCGACAAAG AGAGCAAGCA AATGTGGGCC GAAGGAATAT GGCAGAAATG
    CAAGTACTTG GGGGATTTGA AAGAGGAGAT GAGAATAATG AAGAATTAGA GGAAACTGAA
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86897
    Clone ID Related Accession (Same CDS sequence) XM_030249003.1
    Accession Version XM_030249003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT
    GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG
    GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT
    TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC
    TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT
    GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT
    GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA
    TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT
    CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC
    GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA
    GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC
    GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA
    ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG
    CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT
    TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT
    AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC
    GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT
    GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC
    AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA
    TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG
    TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT
    CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT
    TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA
    CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA
    ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT
    AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC
    AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA
    ACCTACAGTC GCCCAGGGCA GCCCACTCCC TATGCCACCA CTCAACTCAT CCAGGCGAAC
    CTCAGCAACA ATATGAATAA TGGCGCCGGG GACTCCAGCG AGAAGCACTG GAAGCCTCCG
    GGACAGCAGA AACCGGAAGT GGCACCAATT CAGTATAACA TCATGGAGCA AAACAAGCTG
    AACAAAGATT ATCGGGCAAA TGACACGATC CCTCCAACCA TCCCATACAA CCAGTCCTAT
    GACCAGAACA CAGGAGGGTC TTATAACAGC TCAGATCGGG GCAGTAGTAC ATCCGGGAGT
    CAAGGCCACA AGAAAGGGGC ACGGACACCA AAGGCACCCA AGCAGGGTGG CATGAACTGG
    GCAGACTTGC TGCCCCCTCC TCCGGCGCAT CCTCCCCCAC ATAGCAACAG TGAAGAATAC
    AACATGTCCG TAGATGAAAG CTATGATCAA GAAATGCCCT GTCCAGTGCC ACCTGCGCGG
    ATGTATTTGC AACAGGATGA ATTAGAGGAA GAGGAAGATG AAAGAGGCCC CACTCCCCCT
    GTCCGGGGGG CAGCTTCTTC TCCAGCTGCT GTGTCCTACA GCCACCAATC CACGGCCACT
    CTCACCCCTT CTCCCCAGGA AGAGCTCCAG CCCATGTTAC AGGATTGTCC GGAGGACCTC
    GGGCACATGC CCCACCCACC AGACAGGAGG CGGCAGCCCG TGAGTCCTCC TCCACCGCCA
    CGGCCAATTT CCCCACCTCA TACTTACGGC TACATTTCAG GACCCCTTGT CTCAGATATG
    GATACCGATG CCCCAGAAGA GGAGGAGGAC GAAGCTGACA TGGAAGTTGC CAAAATGCAG
    ACCCGAAGGC TGTTGTTACG TGGGCTGGAG CAGACCCCAG CATCCAGTGT TGGGGACCTT
    GAGAGCTCCG TCACTGGGTC CATGATCAAT GGCTGGGGCT CAGCCTCGGA AGAGGACAAC
    ATTTCCAGTG GGCGCTCCAG TGTCAGTTCA TCCGATGGCT CCTTTTTCAC TGACGCTGAT
    TTTGCCCAGG CAGTTGCTGC AGCTGCGGAG TATGCGGGCC TGAAAGTGGC TCGCCGCCAA
    ATGCAAGATG CTGCTGGCCG CCGCCACTTC CATGCCTCTC AGTGCCCAAG GCCCACGAGT
    CCTGTGTCCA CAGACAGCAA CATGAGTGCT GTTGTGATCC AGAAAGCCAG ACCCGCCAAG
    AAGCAGAAAC ACCAGCCAGG ACATCTGCGC AGGGAAGCCT ACGCAGATGA TCTTCCACCC
    CCTCCAGTGC CACCACCTGC TATAAAATCG CCCACTGTCC AGTCCAAGGC ACAGCTGGAG
    GTACGGCCTG TCATGGTGCC AAAACTCGCG TCTATAGAAG CAAGGACAGA TAGATCGTCA
    GACAGAAAAG GAGGCAGTTA CAAGGGGAGA GAAGCTCTGG ATGGAAGACA AGTCACTGAC
    CTGCGAACAA ATCCAAGTGA CCCCAGAGAG GCACAGGAAC AGCCAAATGA CGGGAAAGGC
    CGAGGAACAA AGGCAGCCAA GCGAGACCTT CCACCAGCAA AGACGCACCT CGGCCAAGAG
    GATATTCTTC CGTACTGTAG ACCTACTTTC CCAACGTCAA ACAACCCTAG AGACCCCAGT
    TCCTCCAGCT CTATGTCATC AAGAGGATCA GGGAGCCGAC AAAGAGAGCA AGCAAATGTG
    GGCCGAAGGA ATATGGCAGA AATGCAAGTA CTTGGGGGAT TTGAAAGAGG AGATGAGAAT
    AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104863.1
    CDS97..4986
    Translation

    Target ORF information:

    RefSeq Version XM_030249003.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT
    GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG
    GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT
    TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC
    TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT
    GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT
    GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA
    TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT
    CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC
    GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA
    GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC
    GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA
    ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG
    CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT
    TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT
    AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC
    GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT
    GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC
    AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA
    TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG
    TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT
    CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT
    TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA
    CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA
    ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT
    AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC
    AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA
    ACCTACAGTC GCCCAGGGCA GCCCACTCCC TATGCCACCA CTCAACTCAT CCAGGCGAAC
    CTCAGCAACA ATATGAATAA TGGCGCCGGG GACTCCAGCG AGAAGCACTG GAAGCCTCCG
    GGACAGCAGA AACCGGAAGT GGCACCAATT CAGTATAACA TCATGGAGCA AAACAAGCTG
    AACAAAGATT ATCGGGCAAA TGACACGATC CCTCCAACCA TCCCATACAA CCAGTCCTAT
    GACCAGAACA CAGGAGGGTC TTATAACAGC TCAGATCGGG GCAGTAGTAC ATCCGGGAGT
    CAAGGCCACA AGAAAGGGGC ACGGACACCA AAGGCACCCA AGCAGGGTGG CATGAACTGG
    GCAGACTTGC TGCCCCCTCC TCCGGCGCAT CCTCCCCCAC ATAGCAACAG TGAAGAATAC
    AACATGTCCG TAGATGAAAG CTATGATCAA GAAATGCCCT GTCCAGTGCC ACCTGCGCGG
    ATGTATTTGC AACAGGATGA ATTAGAGGAA GAGGAAGATG AAAGAGGCCC CACTCCCCCT
    GTCCGGGGGG CAGCTTCTTC TCCAGCTGCT GTGTCCTACA GCCACCAATC CACGGCCACT
    CTCACCCCTT CTCCCCAGGA AGAGCTCCAG CCCATGTTAC AGGATTGTCC GGAGGACCTC
    GGGCACATGC CCCACCCACC AGACAGGAGG CGGCAGCCCG TGAGTCCTCC TCCACCGCCA
    CGGCCAATTT CCCCACCTCA TACTTACGGC TACATTTCAG GACCCCTTGT CTCAGATATG
    GATACCGATG CCCCAGAAGA GGAGGAGGAC GAAGCTGACA TGGAAGTTGC CAAAATGCAG
    ACCCGAAGGC TGTTGTTACG TGGGCTGGAG CAGACCCCAG CATCCAGTGT TGGGGACCTT
    GAGAGCTCCG TCACTGGGTC CATGATCAAT GGCTGGGGCT CAGCCTCGGA AGAGGACAAC
    ATTTCCAGTG GGCGCTCCAG TGTCAGTTCA TCCGATGGCT CCTTTTTCAC TGACGCTGAT
    TTTGCCCAGG CAGTTGCTGC AGCTGCGGAG TATGCGGGCC TGAAAGTGGC TCGCCGCCAA
    ATGCAAGATG CTGCTGGCCG CCGCCACTTC CATGCCTCTC AGTGCCCAAG GCCCACGAGT
    CCTGTGTCCA CAGACAGCAA CATGAGTGCT GTTGTGATCC AGAAAGCCAG ACCCGCCAAG
    AAGCAGAAAC ACCAGCCAGG ACATCTGCGC AGGGAAGCCT ACGCAGATGA TCTTCCACCC
    CCTCCAGTGC CACCACCTGC TATAAAATCG CCCACTGTCC AGTCCAAGGC ACAGCTGGAG
    GTACGGCCTG TCATGGTGCC AAAACTCGCG TCTATAGAAG CAAGGACAGA TAGATCGTCA
    GACAGAAAAG GAGGCAGTTA CAAGGGGAGA GAAGCTCTGG ATGGAAGACA AGTCACTGAC
    CTGCGAACAA ATCCAAGTGA CCCCAGAGAG GCACAGGAAC AGCCAAATGA CGGGAAAGGC
    CGAGGAACAA AGGCAGCCAA GCGAGACCTT CCACCAGCAA AGACGCACCT CGGCCAAGAG
    GATATTCTTC CGTACTGTAG ACCTACTTTC CCAACGTCAA ACAACCCTAG AGACCCCAGT
    TCCTCCAGCT CTATGTCATC AAGAGGATCA GGGAGCCGAC AAAGAGAGCA AGCAAATGTG
    GGCCGAAGGA ATATGGCAGA AATGCAAGTA CTTGGGGGAT TTGAAAGAGG AGATGAGAAT
    AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86898
    Clone ID Related Accession (Same CDS sequence) XM_030249005.1
    Accession Version XM_030249005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC
    CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA
    CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA
    CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG
    CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA
    AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA
    GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC
    ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC
    ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA
    ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA
    TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT
    GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC
    TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA
    ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT
    CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA
    CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT
    GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC
    GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA
    GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC
    CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA
    CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT
    AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC
    CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT
    CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT
    CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC
    ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC
    AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTAACT
    TATCAAAGAG GAGGCGAAGC CGTCAGCAGT GGAGGGAGGC CGGGACTCCT CAACATTAGT
    GAACCTGCCA CGCAGCCATG GCTGGCAGAC ACGTGGCCCA ACACAGGCAA CAACCACAAT
    GACTGCTCCA TCAACTGCTG CACGGCCGGC AATGGCAACA GCGACAGCAA CCTGACAACC
    TACAGTCGCC CAGCTGATTG TATAGCCAAT TATAACAACC AACTGGATAA CAAACAAACA
    AATCTGATGC TCCCTGAGTC AACTGTTTAT GGTGATGTGG ACCTTAGTAA CAAAATCAAT
    GAGATGAAAA CCTTCAATAG CCCAAATCTG AAGGACGGGC GTTTTGTCAA TCCATCAGGG
    CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT
    AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA
    GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA
    AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG
    TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG
    GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT
    CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA
    AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT
    GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT
    TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA
    CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT
    CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA
    GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA
    CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC
    AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT
    GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC
    CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC
    AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA
    GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT
    GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG
    CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT
    TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT
    GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC
    AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT
    AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA
    TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA
    GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104865.1
    CDS1366..6177
    Translation

    Target ORF information:

    RefSeq Version XM_030249005.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC
    CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA
    CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA
    CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG
    CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA
    AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA
    GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC
    ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC
    ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA
    ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA
    TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT
    GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC
    TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA
    ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT
    CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA
    CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT
    GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC
    GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA
    GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC
    CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA
    CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT
    AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC
    CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT
    CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT
    CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC
    ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC
    AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTAACT
    TATCAAAGAG GAGGCGAAGC CGTCAGCAGT GGAGGGAGGC CGGGACTCCT CAACATTAGT
    GAACCTGCCA CGCAGCCATG GCTGGCAGAC ACGTGGCCCA ACACAGGCAA CAACCACAAT
    GACTGCTCCA TCAACTGCTG CACGGCCGGC AATGGCAACA GCGACAGCAA CCTGACAACC
    TACAGTCGCC CAGCTGATTG TATAGCCAAT TATAACAACC AACTGGATAA CAAACAAACA
    AATCTGATGC TCCCTGAGTC AACTGTTTAT GGTGATGTGG ACCTTAGTAA CAAAATCAAT
    GAGATGAAAA CCTTCAATAG CCCAAATCTG AAGGACGGGC GTTTTGTCAA TCCATCAGGG
    CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT
    AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA
    GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA
    AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG
    TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG
    GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT
    CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA
    AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT
    GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT
    TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA
    CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT
    CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA
    GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA
    CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC
    AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT
    GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC
    CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC
    AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA
    GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT
    GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG
    CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT
    TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT
    GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC
    AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT
    AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA
    TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA
    GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22089
    Clone ID Related Accession (Same CDS sequence) NM_019413.2
    Accession Version NM_019413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143001.1, Y17793.1, AK040651.1, CK781477.1, AK135605.1 and AC164551.2. On Jan 13, 2007 this sequence version replaced NM_019413.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y17793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC
    CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA
    CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA
    CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG
    CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA
    AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA
    GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC
    ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC
    ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA
    ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA
    TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT
    GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC
    TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA
    ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT
    CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA
    CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT
    GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC
    GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA
    GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC
    CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA
    CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT
    AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC
    CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT
    CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT
    CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC
    ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC
    AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTCCCG
    TCCTTTACCT TCACGCCAAC AGTAACTTAT CAAAGAGGAG GCGAAGCCGT CAGCAGTGGA
    GGGAGGCCGG GACTCCTCAA CATTAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG
    TGGCCCAACA CAGGCAACAA CCACAATGAC TGCTCCATCA ACTGCTGCAC GGCCGGCAAT
    GGCAACAGCG ACAGCAACCT GACAACCTAC AGTCGCCCAG CTGATTGTAT AGCCAATTAT
    AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT
    GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG
    GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC
    CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG
    AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC
    CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA
    TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT
    GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA
    CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC
    ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC
    ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG
    GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT
    CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC
    AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT
    GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT
    CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG
    CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA
    CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA
    CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT
    AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA
    GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC
    GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC
    GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA
    GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA
    GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_062286.2
    CDS992..5830
    Translation

    Target ORF information:

    RefSeq Version NM_019413.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_019413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGAG CCGCCACATT TCGTTGTAAA ACCTCGGGAC
    CAGGTGGTTG CCTTGGGAAG AACTGTGACG TTTCAGTGCG AAGCAACTGG AAATCCTCAA
    CCAGCTATCT TTTGGAGGAG AGAGGGGAGT CAGAATCTAC TTTTCTCCTA CCAGCCTCCA
    CAGTCCTCTA GCCGATTTTC AGTTTCGCAG ACAGGAGACC TCACCATTAC GAACGTCCAG
    CGGTCTGATG TCGGCTACTA CATCTGTCAG ACTTTAAATG TTGCTGGAAG TATCATCACA
    AAGGCCTATT TGGAAGTTAC TGATGTGATT GCAGACCGGC CTCCCCCAGT CATTCGGCAA
    GGTCCTGTGA ATCAGACTGT AGCAGTGGAT GGCACTTTAA TCCTCAGCTG TGTGGCCACA
    GGCAGTCCAG CACCCACAAT TCTGTGGAGG AAGGATGGAG TCCTTGTTTC AACCCAAGAT
    TCTCGGATCA AACAGCTGGA GAGTGGCGTG CTGCAGATCC GCTACGCTAA GCTGGGTGAC
    ACGGGTCGGT ACACCTGCAC CGCATCGACC CCCAGCGGTG AGGCCACGTG GAGCGCTTAC
    ATCGAAGTCC AGGAATTTGG GGTTCCAGTT CAACCCCCAA GACCCACCGA CCCCAACTTA
    ATCCCCAGCG CCCCCTCAAA GCCCGAAGTT ACAGATGTCA GCAAAAACAC GGTCACTTTA
    TCGTGGCAGC CAAACTTGAA CTCAGGAGCA ACCCCAACAT CTTATATTAT AGAAGCCTTC
    AGCCATGCAT CTGGTAGCAG CTGGCAGACG GCGGCAGAAA ATGTGAAAAC AGAAACATTT
    GCCATCAAAG GACTCAAACC TAACGCCATT TACCTTTTCC TGGTGAGGGC AGCAAATGCC
    TATGGAATTA GTGATCCAAG CCAAATATCA GATCCAGTGA AAACGCAAGA TGTCCCACCA
    ACCAGCCAAG GGGTGGACCA CAAGCAGGTC CAAAGAGAAC TGGGGAATGT TGTCTTACAT
    CTGCACAACC CGACCATCCT TTCTTCCTCC TCAGTCGAAG TGCACTGGAC GGTGGACCAA
    CAATCTCAAT ATATTCAAGG CTATAAAATT CTCTACCGCC CATCTGGAGC CAGCCATGGT
    GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACGCCAACGA AAAACAGCGT GGTGATCCCC
    GATCTCAGAA AAGGCGTCAA CTACGAGATT AAGGCTCGCC CATTTTTTAA TGAGTTTCAA
    GGAGCAGACA GTGAAATCAA ATTTGCCAAA ACCTTAGAAG AAGCACCAAG TGCCCCTCCC
    CGAAGTGTAA CTGTATCCAA GAATGATGGA AATGGGACTG CAATCCTGGT CACTTGGCAA
    CCGCCTCCTG AAGACACACA AAATGGAATG GTCCAAGAAT ATAAGGTTTG GTGTCTCGGT
    AATGAAACGA AGTATCACAT AAACAAGACA GTCGATGGCT CGACCTTCTC GGTGGTCATC
    CCCTCTCTGG TTCCTGGGAT CCGATACAGT GTGGAGGTGG CAGCGAGCAC TGGGGCGGGT
    CCCGGGGTGA AGAGCGAACC TCAGTTCATC CAGTTAGATT CTCACGGAAA CCCCGTGTCT
    CCCGAGGACC AAGTCAGCCT TGCTCAGCAG ATCTCCGACG TGGTGAGGCA GCCGGCGTTC
    ATTGCAGGCA TTGGGGCAGC CTGTTGGATT ATCCTCATGG TCTTCAGCAT CTGGCTTTAC
    AGACACCGCA AGAAGAGAAA CGGACTCACC AGCACGTATG CGGGTATCCG GAAAGTCCCG
    TCCTTTACCT TCACGCCAAC AGTAACTTAT CAAAGAGGAG GCGAAGCCGT CAGCAGTGGA
    GGGAGGCCGG GACTCCTCAA CATTAGTGAA CCTGCCACGC AGCCATGGCT GGCAGACACG
    TGGCCCAACA CAGGCAACAA CCACAATGAC TGCTCCATCA ACTGCTGCAC GGCCGGCAAT
    GGCAACAGCG ACAGCAACCT GACAACCTAC AGTCGCCCAG CTGATTGTAT AGCCAATTAT
    AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT
    GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG
    GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC
    CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG
    AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC
    CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA
    TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT
    GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA
    CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC
    ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC
    ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG
    GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT
    CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC
    AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT
    GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT
    CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG
    CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA
    CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA
    CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT
    AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA
    GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC
    GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC
    GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA
    GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA
    GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52697
    Clone ID Related Accession (Same CDS sequence) XM_006522957.2
    Accession Version XM_006522957.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT
    GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG
    GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT
    TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC
    TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT
    GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT
    GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA
    TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT
    CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC
    GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA
    GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC
    GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA
    ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG
    CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT
    TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT
    AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC
    GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT
    GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC
    AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA
    TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG
    TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT
    CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT
    TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA
    CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA
    ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT
    AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC
    AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA
    ACCTACAGTC GCCCAGCTGA TTGTATAGCC AATTATAACA ACCAACTGGA TAACAAACAA
    ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC
    AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA
    GGGCAGCCCA CTCCCTATGC CACCACTCAA CTCATCCAGG CGAACCTCAG CAACAATATG
    AATAATGGCG CCGGGGACTC CAGCGAGAAG CACTGGAAGC CTCCGGGACA GCAGAAACCG
    GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGG
    GCAAATGACA CGATCCCTCC AACCATCCCA TACAACCAGT CCTATGACCA GAACACAGGA
    GGGTCTTATA ACAGCTCAGA TCGGGGCAGT AGTACATCCG GGAGTCAAGG CCACAAGAAA
    GGGGCACGGA CACCAAAGGC ACCCAAGCAG GGTGGCATGA ACTGGGCAGA CTTGCTGCCC
    CCTCCTCCGG CGCATCCTCC CCCACATAGC AACAGTGAAG AATACAACAT GTCCGTAGAT
    GAAAGCTATG ATCAAGAAAT GCCCTGTCCA GTGCCACCTG CGCGGATGTA TTTGCAACAG
    GATGAATTAG AGGAAGAGGA AGATGAAAGA GGCCCCACTC CCCCTGTCCG GGGGGCAGCT
    TCTTCTCCAG CTGCTGTGTC CTACAGCCAC CAATCCACGG CCACTCTCAC CCCTTCTCCC
    CAGGAAGAGC TCCAGCCCAT GTTACAGGAT TGTCCGGAGG ACCTCGGGCA CATGCCCCAC
    CCACCAGACA GGAGGCGGCA GCCCGTGAGT CCTCCTCCAC CGCCACGGCC AATTTCCCCA
    CCTCATACTT ACGGCTACAT TTCAGGACCC CTTGTCTCAG ATATGGATAC CGATGCCCCA
    GAAGAGGAGG AGGACGAAGC TGACATGGAA GTTGCCAAAA TGCAGACCCG AAGGCTGTTG
    TTACGTGGGC TGGAGCAGAC CCCAGCATCC AGTGTTGGGG ACCTTGAGAG CTCCGTCACT
    GGGTCCATGA TCAATGGCTG GGGCTCAGCC TCGGAAGAGG ACAACATTTC CAGTGGGCGC
    TCCAGTGTCA GTTCATCCGA TGGCTCCTTT TTCACTGACG CTGATTTTGC CCAGGCAGTT
    GCTGCAGCTG CGGAGTATGC GGGCCTGAAA GTGGCTCGCC GCCAAATGCA AGATGCTGCT
    GGCCGCCGCC ACTTCCATGC CTCTCAGTGC CCAAGGCCCA CGAGTCCTGT GTCCACAGAC
    AGCAACATGA GTGCTGTTGT GATCCAGAAA GCCAGACCCG CCAAGAAGCA GAAACACCAG
    CCAGGACATC TGCGCAGGGA AGCCTACGCA GATGATCTTC CACCCCCTCC AGTGCCACCA
    CCTGCTATAA AATCGCCCAC TGTCCAGTCC AAGGCACAGC TGGAGGTACG GCCTGTCATG
    GTGCCAAAAC TCGCGTCTAT AGAAGCAAGG ACAGATAGAT CGTCAGACAG AAAAGGAGGC
    AGTTACAAGG GGAGAGAAGC TCTGGATGGA AGACAAGTCA CTGACCTGCG AACAAATCCA
    AGTGACCCCA GAGAGGCACA GGAACAGCCA AATGACGGGA AAGGCCGAGG AACAAAGGCA
    GCCAAGCGAG ACCTTCCACC AGCAAAGACG CACCTCGGCC AAGAGGATAT TCTTCCGTAC
    TGTAGACCTA CTTTCCCAAC GTCAAACAAC CCTAGAGACC CCAGTTCCTC CAGCTCTATG
    TCATCAAGAG GATCAGGGAG CCGACAAAGA GAGCAAGCAA ATGTGGGCCG AAGGAATATG
    GCAGAAATGC AAGTACTTGG GGGATTTGAA AGAGGAGATG AGAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523020.1
    CDS98..5152
    Translation

    Target ORF information:

    RefSeq Version XM_006522957.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522957.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGAGCCGC CACATTTCGT TGTAAAACCT CGGGACCAGG TGGTTGCCTT GGGAAGAACT
    GTGACGTTTC AGTGCGAAGC AACTGGAAAT CCTCAACCAG CTATCTTTTG GAGGAGAGAG
    GGGAGTCAGA ATCTACTTTT CTCCTACCAG CCTCCACAGT CCTCTAGCCG ATTTTCAGTT
    TCGCAGACAG GAGACCTCAC CATTACGAAC GTCCAGCGGT CTGATGTCGG CTACTACATC
    TGTCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAGG CCTATTTGGA AGTTACTGAT
    GTGATTGCAG ACCGGCCTCC CCCAGTCATT CGGCAAGGTC CTGTGAATCA GACTGTAGCA
    GTGGATGGCA CTTTAATCCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACAATTCTG
    TGGAGGAAGG ATGGAGTCCT TGTTTCAACC CAAGATTCTC GGATCAAACA GCTGGAGAGT
    GGCGTGCTGC AGATCCGCTA CGCTAAGCTG GGTGACACGG GTCGGTACAC CTGCACCGCA
    TCGACCCCCA GCGGTGAGGC CACGTGGAGC GCTTACATCG AAGTCCAGGA ATTTGGGGTT
    CCAGTTCAAC CCCCAAGACC CACCGACCCC AACTTAATCC CCAGCGCCCC CTCAAAGCCC
    GAAGTTACAG ATGTCAGCAA AAACACGGTC ACTTTATCGT GGCAGCCAAA CTTGAACTCA
    GGAGCAACCC CAACATCTTA TATTATAGAA GCCTTCAGCC ATGCATCTGG TAGCAGCTGG
    CAGACGGCGG CAGAAAATGT GAAAACAGAA ACATTTGCCA TCAAAGGACT CAAACCTAAC
    GCCATTTACC TTTTCCTGGT GAGGGCAGCA AATGCCTATG GAATTAGTGA TCCAAGCCAA
    ATATCAGATC CAGTGAAAAC GCAAGATGTC CCACCAACCA GCCAAGGGGT GGACCACAAG
    CAGGTCCAAA GAGAACTGGG GAATGTTGTC TTACATCTGC ACAACCCGAC CATCCTTTCT
    TCCTCCTCAG TCGAAGTGCA CTGGACGGTG GACCAACAAT CTCAATATAT TCAAGGCTAT
    AAAATTCTCT ACCGCCCATC TGGAGCCAGC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA
    GTGAGGACGC CAACGAAAAA CAGCGTGGTG ATCCCCGATC TCAGAAAAGG CGTCAACTAC
    GAGATTAAGG CTCGCCCATT TTTTAATGAG TTTCAAGGAG CAGACAGTGA AATCAAATTT
    GCCAAAACCT TAGAAGAAGC ACCAAGTGCC CCTCCCCGAA GTGTAACTGT ATCCAAGAAT
    GATGGAAATG GGACTGCAAT CCTGGTCACT TGGCAACCGC CTCCTGAAGA CACACAAAAT
    GGAATGGTCC AAGAATATAA GGTTTGGTGT CTCGGTAATG AAACGAAGTA TCACATAAAC
    AAGACAGTCG ATGGCTCGAC CTTCTCGGTG GTCATCCCCT CTCTGGTTCC TGGGATCCGA
    TACAGTGTGG AGGTGGCAGC GAGCACTGGG GCGGGTCCCG GGGTGAAGAG CGAACCTCAG
    TTCATCCAGT TAGATTCTCA CGGAAACCCC GTGTCTCCCG AGGACCAAGT CAGCCTTGCT
    CAGCAGATCT CCGACGTGGT GAGGCAGCCG GCGTTCATTG CAGGCATTGG GGCAGCCTGT
    TGGATTATCC TCATGGTCTT CAGCATCTGG CTTTACAGAC ACCGCAAGAA GAGAAACGGA
    CTCACCAGCA CGTATGCGGG TATCCGGAAA GTCCCGTCCT TTACCTTCAC GCCAACAGTA
    ACTTATCAAA GAGGAGGCGA AGCCGTCAGC AGTGGAGGGA GGCCGGGACT CCTCAACATT
    AGTGAACCTG CCACGCAGCC ATGGCTGGCA GACACGTGGC CCAACACAGG CAACAACCAC
    AATGACTGCT CCATCAACTG CTGCACGGCC GGCAATGGCA ACAGCGACAG CAACCTGACA
    ACCTACAGTC GCCCAGCTGA TTGTATAGCC AATTATAACA ACCAACTGGA TAACAAACAA
    ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC
    AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA
    GGGCAGCCCA CTCCCTATGC CACCACTCAA CTCATCCAGG CGAACCTCAG CAACAATATG
    AATAATGGCG CCGGGGACTC CAGCGAGAAG CACTGGAAGC CTCCGGGACA GCAGAAACCG
    GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AGCTGAACAA AGATTATCGG
    GCAAATGACA CGATCCCTCC AACCATCCCA TACAACCAGT CCTATGACCA GAACACAGGA
    GGGTCTTATA ACAGCTCAGA TCGGGGCAGT AGTACATCCG GGAGTCAAGG CCACAAGAAA
    GGGGCACGGA CACCAAAGGC ACCCAAGCAG GGTGGCATGA ACTGGGCAGA CTTGCTGCCC
    CCTCCTCCGG CGCATCCTCC CCCACATAGC AACAGTGAAG AATACAACAT GTCCGTAGAT
    GAAAGCTATG ATCAAGAAAT GCCCTGTCCA GTGCCACCTG CGCGGATGTA TTTGCAACAG
    GATGAATTAG AGGAAGAGGA AGATGAAAGA GGCCCCACTC CCCCTGTCCG GGGGGCAGCT
    TCTTCTCCAG CTGCTGTGTC CTACAGCCAC CAATCCACGG CCACTCTCAC CCCTTCTCCC
    CAGGAAGAGC TCCAGCCCAT GTTACAGGAT TGTCCGGAGG ACCTCGGGCA CATGCCCCAC
    CCACCAGACA GGAGGCGGCA GCCCGTGAGT CCTCCTCCAC CGCCACGGCC AATTTCCCCA
    CCTCATACTT ACGGCTACAT TTCAGGACCC CTTGTCTCAG ATATGGATAC CGATGCCCCA
    GAAGAGGAGG AGGACGAAGC TGACATGGAA GTTGCCAAAA TGCAGACCCG AAGGCTGTTG
    TTACGTGGGC TGGAGCAGAC CCCAGCATCC AGTGTTGGGG ACCTTGAGAG CTCCGTCACT
    GGGTCCATGA TCAATGGCTG GGGCTCAGCC TCGGAAGAGG ACAACATTTC CAGTGGGCGC
    TCCAGTGTCA GTTCATCCGA TGGCTCCTTT TTCACTGACG CTGATTTTGC CCAGGCAGTT
    GCTGCAGCTG CGGAGTATGC GGGCCTGAAA GTGGCTCGCC GCCAAATGCA AGATGCTGCT
    GGCCGCCGCC ACTTCCATGC CTCTCAGTGC CCAAGGCCCA CGAGTCCTGT GTCCACAGAC
    AGCAACATGA GTGCTGTTGT GATCCAGAAA GCCAGACCCG CCAAGAAGCA GAAACACCAG
    CCAGGACATC TGCGCAGGGA AGCCTACGCA GATGATCTTC CACCCCCTCC AGTGCCACCA
    CCTGCTATAA AATCGCCCAC TGTCCAGTCC AAGGCACAGC TGGAGGTACG GCCTGTCATG
    GTGCCAAAAC TCGCGTCTAT AGAAGCAAGG ACAGATAGAT CGTCAGACAG AAAAGGAGGC
    AGTTACAAGG GGAGAGAAGC TCTGGATGGA AGACAAGTCA CTGACCTGCG AACAAATCCA
    AGTGACCCCA GAGAGGCACA GGAACAGCCA AATGACGGGA AAGGCCGAGG AACAAAGGCA
    GCCAAGCGAG ACCTTCCACC AGCAAAGACG CACCTCGGCC AAGAGGATAT TCTTCCGTAC
    TGTAGACCTA CTTTCCCAAC GTCAAACAAC CCTAGAGACC CCAGTTCCTC CAGCTCTATG
    TCATCAAGAG GATCAGGGAG CCGACAAAGA GAGCAAGCAA ATGTGGGCCG AAGGAATATG
    GCAGAAATGC AAGTACTTGG GGGATTTGAA AGAGGAGATG AGAATAATGA AGAATTAGAG
    GAAACTGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52698
    Clone ID Related Accession (Same CDS sequence) XM_006522958.2
    Accession Version XM_006522958.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006522958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT
    GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT
    GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC
    TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT
    CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA
    GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT
    CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG
    GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG
    TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC
    ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC
    ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA
    GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG
    ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC
    GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT
    GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT
    GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT
    GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG
    GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT
    ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA
    GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA
    CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA
    CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA
    ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG
    AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523021.1
    CDS99..5135
    Translation

    Target ORF information:

    RefSeq Version XM_006522958.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522958.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT
    GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT
    GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC
    TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT
    CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA
    GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT
    CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG
    GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG
    TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC
    ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC
    ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA
    GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG
    ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC
    GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT
    GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT
    GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT
    GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG
    GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT
    ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA
    GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA
    CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA
    CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA
    ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG
    AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52700
    Clone ID Related Accession (Same CDS sequence) XM_006522960.3 , XM_006522960.4
    Accession Version XM_006522960.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4977bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG
    GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC
    ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC
    CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC
    CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC
    TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG
    AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT
    GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT
    TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC
    CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA
    AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC
    AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA
    ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT
    GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG
    AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA
    ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC
    GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC
    GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA
    GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT
    TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC
    ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT
    GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT
    CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC
    CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC
    CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC
    TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG
    GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA
    CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC
    AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT
    TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT
    GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG
    GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA
    ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG
    GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG
    CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA
    TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA
    AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA
    TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA
    GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA
    ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT
    AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG
    ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA
    GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT
    CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG
    GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC
    TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG
    GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT
    GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT
    GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC
    TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT
    CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA
    GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT
    CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG
    GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG
    TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC
    ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC
    ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA
    GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG
    ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC
    GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT
    GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT
    GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT
    GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG
    GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT
    ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA
    GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA
    CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA
    CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA
    ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG
    AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523023.1
    CDS393..5369
    Translation

    Target ORF information:

    RefSeq Version XM_006522960.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522960.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG ACCCTGAGGA CCTAGAGAGG
    GGGAACGACA ACGGAACGCC AGCCCCCACC TCTGATAACG ATGACAATTC GCTGGGCTAC
    ACAGGCTCCC GTCTTCGTCA GGAAGATTTT CCACCCCGCA TTGTTGAACA CCCTTCAGAC
    CTGATCGTCT CCAAAGGAGA ACCTGCCACT CTGAACTGTA AAGCCGAAGG CCGCCCCACC
    CCGACGATAG AATGGTACAA AGGTGGGGAA AGAGTGGAGA CGGACAAAGA TGACCCGCGC
    TCACACCGGA TGCTGCTGCC AAGCGGGTCT TTATTTTTCT TACGCATAGT CCATGGACGG
    AAGAGTAGGC CCGACGAAGG GGTCTACATC TGTGTGGCTA GGAATTACCT CGGAGAGGCT
    GTGAGCCACA ACGCATCGCT GGAGGTAGCT ATACTACGGG ATGACTTCAG ACAAAACCCT
    TCAGATGTCA TGGTGGCAGT AGGCGAACCT GCAGTCATGG AATGCCAGCC TCCACGGGGC
    CATCCAGAGC CCACCATCTC CTGGAAAAAG GATGGCTCAC CCCTGGATGA TAAAGATGAA
    AGAATCACTA TACGAGGAGG AAAACTCATG ATCACTTATA CGCGTAAAAG CGATGCTGGC
    AAGTATGTTT GCGTTGGCAC CAACATGGTG GGGGAACGGG AGAGTGAAGT CGCAGAGCTA
    ACCGTTTTAG AGAGACCATC GTTTGTGAAG AGACCCAGTA ATTTGGCAGT GACTGTGGAT
    GACAGTGCAG AATTTAAATG TGAGGCCCGA GGAGACCCTG TGCCTACAGT TCGATGGAGG
    AAAGATGACG GAGAGCTGCC CAAATCCAGA TATGAAATCC GAGATGATCA TACTTTGAAA
    ATTCGGAAGG TGACTGCTGG CGACATGGGA TCATACACTT GTGTGGCAGA AAATATGGTC
    GGGAAAGCTG AAGCTTCCGC TACTTTGACA GTTCAAGTTG GGTCTGAGCC GCCACATTTC
    GTTGTAAAAC CTCGGGACCA GGTGGTTGCC TTGGGAAGAA CTGTGACGTT TCAGTGCGAA
    GCAACTGGAA ATCCTCAACC AGCTATCTTT TGGAGGAGAG AGGGGAGTCA GAATCTACTT
    TTCTCCTACC AGCCTCCACA GTCCTCTAGC CGATTTTCAG TTTCGCAGAC AGGAGACCTC
    ACCATTACGA ACGTCCAGCG GTCTGATGTC GGCTACTACA TCTGTCAGAC TTTAAATGTT
    GCTGGAAGTA TCATCACAAA GGCCTATTTG GAAGTTACTG ATGTGATTGC AGACCGGCCT
    CCCCCAGTCA TTCGGCAAGG TCCTGTGAAT CAGACTGTAG CAGTGGATGG CACTTTAATC
    CTCAGCTGTG TGGCCACAGG CAGTCCAGCA CCCACAATTC TGTGGAGGAA GGATGGAGTC
    CTTGTTTCAA CCCAAGATTC TCGGATCAAA CAGCTGGAGA GTGGCGTGCT GCAGATCCGC
    TACGCTAAGC TGGGTGACAC GGGTCGGTAC ACCTGCACCG CATCGACCCC CAGCGGTGAG
    GCCACGTGGA GCGCTTACAT CGAAGTCCAG GAATTTGGGG TTCCAGTTCA ACCCCCAAGA
    CCCACCGACC CCAACTTAAT CCCCAGCGCC CCCTCAAAGC CCGAAGTTAC AGATGTCAGC
    AAAAACACGG TCACTTTATC GTGGCAGCCA AACTTGAACT CAGGAGCAAC CCCAACATCT
    TATATTATAG AAGCCTTCAG CCATGCATCT GGTAGCAGCT GGCAGACGGC GGCAGAAAAT
    GTGAAAACAG AAACATTTGC CATCAAAGGA CTCAAACCTA ACGCCATTTA CCTTTTCCTG
    GTGAGGGCAG CAAATGCCTA TGGAATTAGT GATCCAAGCC AAATATCAGA TCCAGTGAAA
    ACGCAAGATG TCCCACCAAC CAGCCAAGGG GTGGACCACA AGCAGGTCCA AAGAGAACTG
    GGGAATGTTG TCTTACATCT GCACAACCCG ACCATCCTTT CTTCCTCCTC AGTCGAAGTG
    CACTGGACGG TGGACCAACA ATCTCAATAT ATTCAAGGCT ATAAAATTCT CTACCGCCCA
    TCTGGAGCCA GCCATGGTGA ATCGGAGTGG TTAGTTTTTG AAGTGAGGAC GCCAACGAAA
    AACAGCGTGG TGATCCCCGA TCTCAGAAAA GGCGTCAACT ACGAGATTAA GGCTCGCCCA
    TTTTTTAATG AGTTTCAAGG AGCAGACAGT GAAATCAAAT TTGCCAAAAC CTTAGAAGAA
    GCACCAAGTG CCCCTCCCCG AAGTGTAACT GTATCCAAGA ATGATGGAAA TGGGACTGCA
    ATCCTGGTCA CTTGGCAACC GCCTCCTGAA GACACACAAA ATGGAATGGT CCAAGAATAT
    AAGGTTTGGT GTCTCGGTAA TGAAACGAAG TATCACATAA ACAAGACAGT CGATGGCTCG
    ACCTTCTCGG TGGTCATCCC CTCTCTGGTT CCTGGGATCC GATACAGTGT GGAGGTGGCA
    GCGAGCACTG GGGCGGGTCC CGGGGTGAAG AGCGAACCTC AGTTCATCCA GTTAGATTCT
    CACGGAAACC CCGTGTCTCC CGAGGACCAA GTCAGCCTTG CTCAGCAGAT CTCCGACGTG
    GTGAGGCAGC CGGCGTTCAT TGCAGGCATT GGGGCAGCCT GTTGGATTAT CCTCATGGTC
    TTCAGCATCT GGCTTTACAG ACACCGCAAG AAGAGAAACG GACTCACCAG CACGTATGCG
    GGTATCCGGA AAGTCCCGTC CTTTACCTTC ACGCCAACAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGCT
    GATTGTATAG CCAATTATAA CAACCAACTG GATAACAAAC AAACAAATCT GATGCTCCCT
    GAGTCAACTG TTTATGGTGA TGTGGACCTT AGTAACAAAA TCAATGAGAT GAAAACCTTC
    AATAGCCCAA ATCTGAAGGA CGGGCGTTTT GTCAATCCAT CAGGGCAGCC CACTCCCTAT
    GCCACCACTC AACTCATCCA GGCGAACCTC AGCAACAATA TGAATAATGG CGCCGGGGAC
    TCCAGCGAGA AGCACTGGAA GCCTCCGGGA CAGCAGAAAC CGGAAGTGGC ACCAATTCAG
    TATAACATCA TGGAGCAAAA CAAGCTGAAC AAAGATTATC GGGCAAATGA CACGATCCCT
    CCAACCATCC CATACAACCA GTCCTATGAC CAGAACACAG GAGGGTCTTA TAACAGCTCA
    GATCGGGGCA GTAGTACATC CGGGAGTCAA GGCCACAAGA AAGGGGCACG GACACCAAAG
    GCACCCAAGC AGGGTGGCAT GAACTGGGCA GACTTGCTGC CCCCTCCTCC GGCGCATCCT
    CCCCCACATA GCAACAGTGA AGAATACAAC ATGTCCGTAG ATGAAAGCTA TGATCAAGAA
    ATGCCCTGTC CAGTGCCACC TGCGCGGATG TATTTGCAAC AGGATGAATT AGAGGAAGAG
    GAAGATGAAA GAGGCCCCAC TCCCCCTGTC CGGGGGGCAG CTTCTTCTCC AGCTGCTGTG
    TCCTACAGCC ACCAATCCAC GGCCACTCTC ACCCCTTCTC CCCAGGAAGA GCTCCAGCCC
    ATGTTACAGG ATTGTCCGGA GGACCTCGGG CACATGCCCC ACCCACCAGA CAGGAGGCGG
    CAGCCCGTGA GTCCTCCTCC ACCGCCACGG CCAATTTCCC CACCTCATAC TTACGGCTAC
    ATTTCAGGAC CCCTTGTCTC AGATATGGAT ACCGATGCCC CAGAAGAGGA GGAGGACGAA
    GCTGACATGG AAGTTGCCAA AATGCAGACC CGAAGGCTGT TGTTACGTGG GCTGGAGCAG
    ACCCCAGCAT CCAGTGTTGG GGACCTTGAG AGCTCCGTCA CTGGGTCCAT GATCAATGGC
    TGGGGCTCAG CCTCGGAAGA GGACAACATT TCCAGTGGGC GCTCCAGTGT CAGTTCATCC
    GATGGCTCCT TTTTCACTGA CGCTGATTTT GCCCAGGCAG TTGCTGCAGC TGCGGAGTAT
    GCGGGCCTGA AAGTGGCTCG CCGCCAAATG CAAGATGCTG CTGGCCGCCG CCACTTCCAT
    GCCTCTCAGT GCCCAAGGCC CACGAGTCCT GTGTCCACAG ACAGCAACAT GAGTGCTGTT
    GTGATCCAGA AAGCCAGACC CGCCAAGAAG CAGAAACACC AGCCAGGACA TCTGCGCAGG
    GAAGCCTACG CAGATGATCT TCCACCCCCT CCAGTGCCAC CACCTGCTAT AAAATCGCCC
    ACTGTCCAGT CCAAGGCACA GCTGGAGGTA CGGCCTGTCA TGGTGCCAAA ACTCGCGTCT
    ATAGAAGCAA GGACAGATAG ATCGTCAGAC AGAAAAGGAG GCAGTTACAA GGGGAGAGAA
    GCTCTGGATG GAAGACAAGT CACTGACCTG CGAACAAATC CAAGTGACCC CAGAGAGGCA
    CAGGAACAGC CAAATGACGG GAAAGGCCGA GGAACAAAGG CAGCCAAGCG AGACCTTCCA
    CCAGCAAAGA CGCACCTCGG CCAAGAGGAT ATTCTTCCGT ACTGTAGACC TACTTTCCCA
    ACGTCAAACA ACCCTAGAGA CCCCAGTTCC TCCAGCTCTA TGTCATCAAG AGGATCAGGG
    AGCCGACAAA GAGAGCAAGC AAATGTGGGC CGAAGGAATA TGGCAGAAAT GCAAGTACTT
    GGGGGATTTG AAAGAGGAGA TGAGAATAAT GAAGAATTAG AGGAAACTGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52701
    Clone ID Related Accession (Same CDS sequence) XM_006522961.2 , XM_006522961.3
    Accession Version XM_006522961.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA
    GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG
    TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG
    CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC
    GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA
    TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG
    GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC
    ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA
    GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT
    GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA
    CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT
    AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG
    CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT
    GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT
    TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG
    GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT
    CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT
    CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC
    CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC
    ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC
    ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT
    GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT
    TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC
    TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT
    TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA
    TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT
    GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA
    CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA
    CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC
    CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT
    GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC
    CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT
    CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT
    CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG
    CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC
    GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC
    ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG
    GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG
    TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG
    TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT
    TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC
    CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT
    GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC
    ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC
    AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC
    ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC
    TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG
    CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC
    AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT
    ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT
    GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC
    AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG
    CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC
    CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA
    TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT
    CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT
    CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT
    GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT
    GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT
    GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG
    GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC
    ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG
    GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA
    AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC
    AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT
    GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG
    GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA
    GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA
    CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT
    GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC
    CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT
    AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG
    CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA
    GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523024.1
    CDS478..5379
    Translation

    Target ORF information:

    RefSeq Version XM_006522961.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522961.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTTACTGAT GCTGCTCTTC TCAATGGCTG AAATAACTTC CCCTGTGGGT 
    GTCTCCGGCA GAATGAGAGC TGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGTCAGGAAG ATTTTCCACC CCGCATTGTT GAACACCCTT CAGACCTGAT CGTCTCCAAA
    GGAGAACCTG CCACTCTGAA CTGTAAAGCC GAAGGCCGCC CCACCCCGAC GATAGAATGG
    TACAAAGGTG GGGAAAGAGT GGAGACGGAC AAAGATGACC CGCGCTCACA CCGGATGCTG
    CTGCCAAGCG GGTCTTTATT TTTCTTACGC ATAGTCCATG GACGGAAGAG TAGGCCCGAC
    GAAGGGGTCT ACATCTGTGT GGCTAGGAAT TACCTCGGAG AGGCTGTGAG CCACAACGCA
    TCGCTGGAGG TAGCTATACT ACGGGATGAC TTCAGACAAA ACCCTTCAGA TGTCATGGTG
    GCAGTAGGCG AACCTGCAGT CATGGAATGC CAGCCTCCAC GGGGCCATCC AGAGCCCACC
    ATCTCCTGGA AAAAGGATGG CTCACCCCTG GATGATAAAG ATGAAAGAAT CACTATACGA
    GGAGGAAAAC TCATGATCAC TTATACGCGT AAAAGCGATG CTGGCAAGTA TGTTTGCGTT
    GGCACCAACA TGGTGGGGGA ACGGGAGAGT GAAGTCGCAG AGCTAACCGT TTTAGAGAGA
    CCATCGTTTG TGAAGAGACC CAGTAATTTG GCAGTGACTG TGGATGACAG TGCAGAATTT
    AAATGTGAGG CCCGAGGAGA CCCTGTGCCT ACAGTTCGAT GGAGGAAAGA TGACGGAGAG
    CTGCCCAAAT CCAGATATGA AATCCGAGAT GATCATACTT TGAAAATTCG GAAGGTGACT
    GCTGGCGACA TGGGATCATA CACTTGTGTG GCAGAAAATA TGGTCGGGAA AGCTGAAGCT
    TCCGCTACTT TGACAGTTCA AGTTGGGTCT GAGCCGCCAC ATTTCGTTGT AAAACCTCGG
    GACCAGGTGG TTGCCTTGGG AAGAACTGTG ACGTTTCAGT GCGAAGCAAC TGGAAATCCT
    CAACCAGCTA TCTTTTGGAG GAGAGAGGGG AGTCAGAATC TACTTTTCTC CTACCAGCCT
    CCACAGTCCT CTAGCCGATT TTCAGTTTCG CAGACAGGAG ACCTCACCAT TACGAACGTC
    CAGCGGTCTG ATGTCGGCTA CTACATCTGT CAGACTTTAA ATGTTGCTGG AAGTATCATC
    ACAAAGGCCT ATTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC AGTCATTCGG
    CAAGGTCCTG TGAATCAGAC TGTAGCAGTG GATGGCACTT TAATCCTCAG CTGTGTGGCC
    ACAGGCAGTC CAGCACCCAC AATTCTGTGG AGGAAGGATG GAGTCCTTGT TTCAACCCAA
    GATTCTCGGA TCAAACAGCT GGAGAGTGGC GTGCTGCAGA TCCGCTACGC TAAGCTGGGT
    GACACGGGTC GGTACACCTG CACCGCATCG ACCCCCAGCG GTGAGGCCAC GTGGAGCGCT
    TACATCGAAG TCCAGGAATT TGGGGTTCCA GTTCAACCCC CAAGACCCAC CGACCCCAAC
    TTAATCCCCA GCGCCCCCTC AAAGCCCGAA GTTACAGATG TCAGCAAAAA CACGGTCACT
    TTATCGTGGC AGCCAAACTT GAACTCAGGA GCAACCCCAA CATCTTATAT TATAGAAGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAG ACGGCGGCAG AAAATGTGAA AACAGAAACA
    TTTGCCATCA AAGGACTCAA ACCTAACGCC ATTTACCTTT TCCTGGTGAG GGCAGCAAAT
    GCCTATGGAA TTAGTGATCC AAGCCAAATA TCAGATCCAG TGAAAACGCA AGATGTCCCA
    CCAACCAGCC AAGGGGTGGA CCACAAGCAG GTCCAAAGAG AACTGGGGAA TGTTGTCTTA
    CATCTGCACA ACCCGACCAT CCTTTCTTCC TCCTCAGTCG AAGTGCACTG GACGGTGGAC
    CAACAATCTC AATATATTCA AGGCTATAAA ATTCTCTACC GCCCATCTGG AGCCAGCCAT
    GGTGAATCGG AGTGGTTAGT TTTTGAAGTG AGGACGCCAA CGAAAAACAG CGTGGTGATC
    CCCGATCTCA GAAAAGGCGT CAACTACGAG ATTAAGGCTC GCCCATTTTT TAATGAGTTT
    CAAGGAGCAG ACAGTGAAAT CAAATTTGCC AAAACCTTAG AAGAAGCACC AAGTGCCCCT
    CCCCGAAGTG TAACTGTATC CAAGAATGAT GGAAATGGGA CTGCAATCCT GGTCACTTGG
    CAACCGCCTC CTGAAGACAC ACAAAATGGA ATGGTCCAAG AATATAAGGT TTGGTGTCTC
    GGTAATGAAA CGAAGTATCA CATAAACAAG ACAGTCGATG GCTCGACCTT CTCGGTGGTC
    ATCCCCTCTC TGGTTCCTGG GATCCGATAC AGTGTGGAGG TGGCAGCGAG CACTGGGGCG
    GGTCCCGGGG TGAAGAGCGA ACCTCAGTTC ATCCAGTTAG ATTCTCACGG AAACCCCGTG
    TCTCCCGAGG ACCAAGTCAG CCTTGCTCAG CAGATCTCCG ACGTGGTGAG GCAGCCGGCG
    TTCATTGCAG GCATTGGGGC AGCCTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGCTT
    TACAGACACC GCAAGAAGAG AAACGGACTC ACCAGCACGT ATGCGGGTAT CCGGAAAGTC
    CCGTCCTTTA CCTTCACGCC AACAGTAACT TATCAAAGAG GAGGCGAAGC CGTCAGCAGT
    GGAGGGAGGC CGGGACTCCT CAACATTAGT GAACCTGCCA CGCAGCCATG GCTGGCAGAC
    ACGTGGCCCA ACACAGGCAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACGGCCGGC
    AATGGCAACA GCGACAGCAA CCTGACAACC TACAGTCGCC CAGCTGATTG TATAGCCAAT
    TATAACAACC AACTGGATAA CAAACAAACA AATCTGATGC TCCCTGAGTC AACTGTTTAT
    GGTGATGTGG ACCTTAGTAA CAAAATCAAT GAGATGAAAA CCTTCAATAG CCCAAATCTG
    AAGGACGGGC GTTTTGTCAA TCCATCAGGG CAGCCCACTC CCTATGCCAC CACTCAACTC
    ATCCAGGCGA ACCTCAGCAA CAATATGAAT AATGGCGCCG GGGACTCCAG CGAGAAGCAC
    TGGAAGCCTC CGGGACAGCA GAAACCGGAA GTGGCACCAA TTCAGTATAA CATCATGGAG
    CAAAACAAGC TGAACAAAGA TTATCGGGCA AATGACACGA TCCCTCCAAC CATCCCATAC
    AACCAGTCCT ATGACCAGAA CACAGGAGGG TCTTATAACA GCTCAGATCG GGGCAGTAGT
    ACATCCGGGA GTCAAGGCCA CAAGAAAGGG GCACGGACAC CAAAGGCACC CAAGCAGGGT
    GGCATGAACT GGGCAGACTT GCTGCCCCCT CCTCCGGCGC ATCCTCCCCC ACATAGCAAC
    AGTGAAGAAT ACAACATGTC CGTAGATGAA AGCTATGATC AAGAAATGCC CTGTCCAGTG
    CCACCTGCGC GGATGTATTT GCAACAGGAT GAATTAGAGG AAGAGGAAGA TGAAAGAGGC
    CCCACTCCCC CTGTCCGGGG GGCAGCTTCT TCTCCAGCTG CTGTGTCCTA CAGCCACCAA
    TCCACGGCCA CTCTCACCCC TTCTCCCCAG GAAGAGCTCC AGCCCATGTT ACAGGATTGT
    CCGGAGGACC TCGGGCACAT GCCCCACCCA CCAGACAGGA GGCGGCAGCC CGTGAGTCCT
    CCTCCACCGC CACGGCCAAT TTCCCCACCT CATACTTACG GCTACATTTC AGGACCCCTT
    GTCTCAGATA TGGATACCGA TGCCCCAGAA GAGGAGGAGG ACGAAGCTGA CATGGAAGTT
    GCCAAAATGC AGACCCGAAG GCTGTTGTTA CGTGGGCTGG AGCAGACCCC AGCATCCAGT
    GTTGGGGACC TTGAGAGCTC CGTCACTGGG TCCATGATCA ATGGCTGGGG CTCAGCCTCG
    GAAGAGGACA ACATTTCCAG TGGGCGCTCC AGTGTCAGTT CATCCGATGG CTCCTTTTTC
    ACTGACGCTG ATTTTGCCCA GGCAGTTGCT GCAGCTGCGG AGTATGCGGG CCTGAAAGTG
    GCTCGCCGCC AAATGCAAGA TGCTGCTGGC CGCCGCCACT TCCATGCCTC TCAGTGCCCA
    AGGCCCACGA GTCCTGTGTC CACAGACAGC AACATGAGTG CTGTTGTGAT CCAGAAAGCC
    AGACCCGCCA AGAAGCAGAA ACACCAGCCA GGACATCTGC GCAGGGAAGC CTACGCAGAT
    GATCTTCCAC CCCCTCCAGT GCCACCACCT GCTATAAAAT CGCCCACTGT CCAGTCCAAG
    GCACAGCTGG AGGTACGGCC TGTCATGGTG CCAAAACTCG CGTCTATAGA AGCAAGGACA
    GATAGATCGT CAGACAGAAA AGGAGGCAGT TACAAGGGGA GAGAAGCTCT GGATGGAAGA
    CAAGTCACTG ACCTGCGAAC AAATCCAAGT GACCCCAGAG AGGCACAGGA ACAGCCAAAT
    GACGGGAAAG GCCGAGGAAC AAAGGCAGCC AAGCGAGACC TTCCACCAGC AAAGACGCAC
    CTCGGCCAAG AGGATATTCT TCCGTACTGT AGACCTACTT TCCCAACGTC AAACAACCCT
    AGAGACCCCA GTTCCTCCAG CTCTATGTCA TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG
    CAAGCAAATG TGGGCCGAAG GAATATGGCA GAAATGCAAG TACTTGGGGG ATTTGAAAGA
    GGAGATGAGA ATAATGAAGA ATTAGAGGAA ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52702
    Clone ID Related Accession (Same CDS sequence) XM_006522962.4 , XM_006522962.3
    Accession Version XM_006522962.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522962.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC
    AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC
    AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC
    CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG
    AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC
    CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA
    TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT
    GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA
    CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC
    ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC
    ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG
    GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT
    CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC
    AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT
    GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT
    CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG
    CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA
    CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA
    CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT
    AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA
    GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC
    GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC
    GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA
    GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA
    GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523025.1
    CDS97..4995
    Translation

    Target ORF information:

    RefSeq Version XM_006522962.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522962.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TCCCGTCCTT TACCTTCACG
    CCAACAGTAA CTTATCAAAG AGGAGGCGAA GCCGTCAGCA GTGGAGGGAG GCCGGGACTC
    CTCAACATTA GTGAACCTGC CACGCAGCCA TGGCTGGCAG ACACGTGGCC CAACACAGGC
    AACAACCACA ATGACTGCTC CATCAACTGC TGCACGGCCG GCAATGGCAA CAGCGACAGC
    AACCTGACAA CCTACAGTCG CCCAGGGCAG CCCACTCCCT ATGCCACCAC TCAACTCATC
    CAGGCGAACC TCAGCAACAA TATGAATAAT GGCGCCGGGG ACTCCAGCGA GAAGCACTGG
    AAGCCTCCGG GACAGCAGAA ACCGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA
    AACAAGCTGA ACAAAGATTA TCGGGCAAAT GACACGATCC CTCCAACCAT CCCATACAAC
    CAGTCCTATG ACCAGAACAC AGGAGGGTCT TATAACAGCT CAGATCGGGG CAGTAGTACA
    TCCGGGAGTC AAGGCCACAA GAAAGGGGCA CGGACACCAA AGGCACCCAA GCAGGGTGGC
    ATGAACTGGG CAGACTTGCT GCCCCCTCCT CCGGCGCATC CTCCCCCACA TAGCAACAGT
    GAAGAATACA ACATGTCCGT AGATGAAAGC TATGATCAAG AAATGCCCTG TCCAGTGCCA
    CCTGCGCGGA TGTATTTGCA ACAGGATGAA TTAGAGGAAG AGGAAGATGA AAGAGGCCCC
    ACTCCCCCTG TCCGGGGGGC AGCTTCTTCT CCAGCTGCTG TGTCCTACAG CCACCAATCC
    ACGGCCACTC TCACCCCTTC TCCCCAGGAA GAGCTCCAGC CCATGTTACA GGATTGTCCG
    GAGGACCTCG GGCACATGCC CCACCCACCA GACAGGAGGC GGCAGCCCGT GAGTCCTCCT
    CCACCGCCAC GGCCAATTTC CCCACCTCAT ACTTACGGCT ACATTTCAGG ACCCCTTGTC
    TCAGATATGG ATACCGATGC CCCAGAAGAG GAGGAGGACG AAGCTGACAT GGAAGTTGCC
    AAAATGCAGA CCCGAAGGCT GTTGTTACGT GGGCTGGAGC AGACCCCAGC ATCCAGTGTT
    GGGGACCTTG AGAGCTCCGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCGGAA
    GAGGACAACA TTTCCAGTGG GCGCTCCAGT GTCAGTTCAT CCGATGGCTC CTTTTTCACT
    GACGCTGATT TTGCCCAGGC AGTTGCTGCA GCTGCGGAGT ATGCGGGCCT GAAAGTGGCT
    CGCCGCCAAA TGCAAGATGC TGCTGGCCGC CGCCACTTCC ATGCCTCTCA GTGCCCAAGG
    CCCACGAGTC CTGTGTCCAC AGACAGCAAC ATGAGTGCTG TTGTGATCCA GAAAGCCAGA
    CCCGCCAAGA AGCAGAAACA CCAGCCAGGA CATCTGCGCA GGGAAGCCTA CGCAGATGAT
    CTTCCACCCC CTCCAGTGCC ACCACCTGCT ATAAAATCGC CCACTGTCCA GTCCAAGGCA
    CAGCTGGAGG TACGGCCTGT CATGGTGCCA AAACTCGCGT CTATAGAAGC AAGGACAGAT
    AGATCGTCAG ACAGAAAAGG AGGCAGTTAC AAGGGGAGAG AAGCTCTGGA TGGAAGACAA
    GTCACTGACC TGCGAACAAA TCCAAGTGAC CCCAGAGAGG CACAGGAACA GCCAAATGAC
    GGGAAAGGCC GAGGAACAAA GGCAGCCAAG CGAGACCTTC CACCAGCAAA GACGCACCTC
    GGCCAAGAGG ATATTCTTCC GTACTGTAGA CCTACTTTCC CAACGTCAAA CAACCCTAGA
    GACCCCAGTT CCTCCAGCTC TATGTCATCA AGAGGATCAG GGAGCCGACA AAGAGAGCAA
    GCAAATGTGG GCCGAAGGAA TATGGCAGAA ATGCAAGTAC TTGGGGGATT TGAAAGAGGA
    GATGAGAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52703
    Clone ID Related Accession (Same CDS sequence) XM_006522963.3 , XM_006522963.4
    Accession Version XM_006522963.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA
    GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT
    GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT
    GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC
    GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC
    TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG
    GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC
    GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG
    AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA
    CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC
    ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT
    GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG
    GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA
    TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC
    ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT
    TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG
    GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT
    ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC
    TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT
    GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC
    TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT
    GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT
    CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG
    ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT
    CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA
    GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC
    AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG
    CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT
    GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC
    AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA
    ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC
    CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC
    AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT
    CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG
    GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC
    AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA
    GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT
    GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT
    CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA
    ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT
    CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG
    GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG
    GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA
    GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC
    CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT
    ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG
    CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC
    AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC
    AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC
    CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG
    GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG
    CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG
    AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT
    CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG
    CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC
    TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG
    AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC
    TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA
    TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG
    CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC
    CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC
    ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC
    CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG
    CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT
    ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG
    CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC
    CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC
    AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT
    GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC
    CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG
    AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC
    AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA
    CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG
    GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG
    TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT
    GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA
    GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA
    GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC
    AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT
    GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523026.1
    CDS392..5284
    Translation

    Target ORF information:

    RefSeq Version XM_006522963.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522963.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGAGGGA TAAAGATGAA ATGGAAACAC CTTCCTCTTC TGGTCATATC TCTCCTCACT 
    CTATCAAGAA AACAGCTCCT TCTGGCCCAG CTTATCCCAG GCTCCCGTCT TCGTCAGGAA
    GATTTTCCAC CCCGCATTGT TGAACACCCT TCAGACCTGA TCGTCTCCAA AGGAGAACCT
    GCCACTCTGA ACTGTAAAGC CGAAGGCCGC CCCACCCCGA CGATAGAATG GTACAAAGGT
    GGGGAAAGAG TGGAGACGGA CAAAGATGAC CCGCGCTCAC ACCGGATGCT GCTGCCAAGC
    GGGTCTTTAT TTTTCTTACG CATAGTCCAT GGACGGAAGA GTAGGCCCGA CGAAGGGGTC
    TACATCTGTG TGGCTAGGAA TTACCTCGGA GAGGCTGTGA GCCACAACGC ATCGCTGGAG
    GTAGCTATAC TACGGGATGA CTTCAGACAA AACCCTTCAG ATGTCATGGT GGCAGTAGGC
    GAACCTGCAG TCATGGAATG CCAGCCTCCA CGGGGCCATC CAGAGCCCAC CATCTCCTGG
    AAAAAGGATG GCTCACCCCT GGATGATAAA GATGAAAGAA TCACTATACG AGGAGGAAAA
    CTCATGATCA CTTATACGCG TAAAAGCGAT GCTGGCAAGT ATGTTTGCGT TGGCACCAAC
    ATGGTGGGGG AACGGGAGAG TGAAGTCGCA GAGCTAACCG TTTTAGAGAG ACCATCGTTT
    GTGAAGAGAC CCAGTAATTT GGCAGTGACT GTGGATGACA GTGCAGAATT TAAATGTGAG
    GCCCGAGGAG ACCCTGTGCC TACAGTTCGA TGGAGGAAAG ATGACGGAGA GCTGCCCAAA
    TCCAGATATG AAATCCGAGA TGATCATACT TTGAAAATTC GGAAGGTGAC TGCTGGCGAC
    ATGGGATCAT ACACTTGTGT GGCAGAAAAT ATGGTCGGGA AAGCTGAAGC TTCCGCTACT
    TTGACAGTTC AAGTTGGGTC TGAGCCGCCA CATTTCGTTG TAAAACCTCG GGACCAGGTG
    GTTGCCTTGG GAAGAACTGT GACGTTTCAG TGCGAAGCAA CTGGAAATCC TCAACCAGCT
    ATCTTTTGGA GGAGAGAGGG GAGTCAGAAT CTACTTTTCT CCTACCAGCC TCCACAGTCC
    TCTAGCCGAT TTTCAGTTTC GCAGACAGGA GACCTCACCA TTACGAACGT CCAGCGGTCT
    GATGTCGGCT ACTACATCTG TCAGACTTTA AATGTTGCTG GAAGTATCAT CACAAAGGCC
    TATTTGGAAG TTACTGATGT GATTGCAGAC CGGCCTCCCC CAGTCATTCG GCAAGGTCCT
    GTGAATCAGA CTGTAGCAGT GGATGGCACT TTAATCCTCA GCTGTGTGGC CACAGGCAGT
    CCAGCACCCA CAATTCTGTG GAGGAAGGAT GGAGTCCTTG TTTCAACCCA AGATTCTCGG
    ATCAAACAGC TGGAGAGTGG CGTGCTGCAG ATCCGCTACG CTAAGCTGGG TGACACGGGT
    CGGTACACCT GCACCGCATC GACCCCCAGC GGTGAGGCCA CGTGGAGCGC TTACATCGAA
    GTCCAGGAAT TTGGGGTTCC AGTTCAACCC CCAAGACCCA CCGACCCCAA CTTAATCCCC
    AGCGCCCCCT CAAAGCCCGA AGTTACAGAT GTCAGCAAAA ACACGGTCAC TTTATCGTGG
    CAGCCAAACT TGAACTCAGG AGCAACCCCA ACATCTTATA TTATAGAAGC CTTCAGCCAT
    GCATCTGGTA GCAGCTGGCA GACGGCGGCA GAAAATGTGA AAACAGAAAC ATTTGCCATC
    AAAGGACTCA AACCTAACGC CATTTACCTT TTCCTGGTGA GGGCAGCAAA TGCCTATGGA
    ATTAGTGATC CAAGCCAAAT ATCAGATCCA GTGAAAACGC AAGATGTCCC ACCAACCAGC
    CAAGGGGTGG ACCACAAGCA GGTCCAAAGA GAACTGGGGA ATGTTGTCTT ACATCTGCAC
    AACCCGACCA TCCTTTCTTC CTCCTCAGTC GAAGTGCACT GGACGGTGGA CCAACAATCT
    CAATATATTC AAGGCTATAA AATTCTCTAC CGCCCATCTG GAGCCAGCCA TGGTGAATCG
    GAGTGGTTAG TTTTTGAAGT GAGGACGCCA ACGAAAAACA GCGTGGTGAT CCCCGATCTC
    AGAAAAGGCG TCAACTACGA GATTAAGGCT CGCCCATTTT TTAATGAGTT TCAAGGAGCA
    GACAGTGAAA TCAAATTTGC CAAAACCTTA GAAGAAGCAC CAAGTGCCCC TCCCCGAAGT
    GTAACTGTAT CCAAGAATGA TGGAAATGGG ACTGCAATCC TGGTCACTTG GCAACCGCCT
    CCTGAAGACA CACAAAATGG AATGGTCCAA GAATATAAGG TTTGGTGTCT CGGTAATGAA
    ACGAAGTATC ACATAAACAA GACAGTCGAT GGCTCGACCT TCTCGGTGGT CATCCCCTCT
    CTGGTTCCTG GGATCCGATA CAGTGTGGAG GTGGCAGCGA GCACTGGGGC GGGTCCCGGG
    GTGAAGAGCG AACCTCAGTT CATCCAGTTA GATTCTCACG GAAACCCCGT GTCTCCCGAG
    GACCAAGTCA GCCTTGCTCA GCAGATCTCC GACGTGGTGA GGCAGCCGGC GTTCATTGCA
    GGCATTGGGG CAGCCTGTTG GATTATCCTC ATGGTCTTCA GCATCTGGCT TTACAGACAC
    CGCAAGAAGA GAAACGGACT CACCAGCACG TATGCGGGTA TCCGGAAAGT CCCGTCCTTT
    ACCTTCACGC CAACAGTAAC TTATCAAAGA GGAGGCGAAG CCGTCAGCAG TGGAGGGAGG
    CCGGGACTCC TCAACATTAG TGAACCTGCC ACGCAGCCAT GGCTGGCAGA CACGTGGCCC
    AACACAGGCA ACAACCACAA TGACTGCTCC ATCAACTGCT GCACGGCCGG CAATGGCAAC
    AGCGACAGCA ACCTGACAAC CTACAGTCGC CCAGCTGATT GTATAGCCAA TTATAACAAC
    CAACTGGATA ACAAACAAAC AAATCTGATG CTCCCTGAGT CAACTGTTTA TGGTGATGTG
    GACCTTAGTA ACAAAATCAA TGAGATGAAA ACCTTCAATA GCCCAAATCT GAAGGACGGG
    CGTTTTGTCA ATCCATCAGG GCAGCCCACT CCCTATGCCA CCACTCAACT CATCCAGGCG
    AACCTCAGCA ACAATATGAA TAATGGCGCC GGGGACTCCA GCGAGAAGCA CTGGAAGCCT
    CCGGGACAGC AGAAACCGGA AGTGGCACCA ATTCAGTATA ACATCATGGA GCAAAACAAG
    CTGAACAAAG ATTATCGGGC AAATGACACG ATCCCTCCAA CCATCCCATA CAACCAGTCC
    TATGACCAGA ACACAGGAGG GTCTTATAAC AGCTCAGATC GGGGCAGTAG TACATCCGGG
    AGTCAAGGCC ACAAGAAAGG GGCACGGACA CCAAAGGCAC CCAAGCAGGG TGGCATGAAC
    TGGGCAGACT TGCTGCCCCC TCCTCCGGCG CATCCTCCCC CACATAGCAA CAGTGAAGAA
    TACAACATGT CCGTAGATGA AAGCTATGAT CAAGAAATGC CCTGTCCAGT GCCACCTGCG
    CGGATGTATT TGCAACAGGA TGAATTAGAG GAAGAGGAAG ATGAAAGAGG CCCCACTCCC
    CCTGTCCGGG GGGCAGCTTC TTCTCCAGCT GCTGTGTCCT ACAGCCACCA ATCCACGGCC
    ACTCTCACCC CTTCTCCCCA GGAAGAGCTC CAGCCCATGT TACAGGATTG TCCGGAGGAC
    CTCGGGCACA TGCCCCACCC ACCAGACAGG AGGCGGCAGC CCGTGAGTCC TCCTCCACCG
    CCACGGCCAA TTTCCCCACC TCATACTTAC GGCTACATTT CAGGACCCCT TGTCTCAGAT
    ATGGATACCG ATGCCCCAGA AGAGGAGGAG GACGAAGCTG ACATGGAAGT TGCCAAAATG
    CAGACCCGAA GGCTGTTGTT ACGTGGGCTG GAGCAGACCC CAGCATCCAG TGTTGGGGAC
    CTTGAGAGCT CCGTCACTGG GTCCATGATC AATGGCTGGG GCTCAGCCTC GGAAGAGGAC
    AACATTTCCA GTGGGCGCTC CAGTGTCAGT TCATCCGATG GCTCCTTTTT CACTGACGCT
    GATTTTGCCC AGGCAGTTGC TGCAGCTGCG GAGTATGCGG GCCTGAAAGT GGCTCGCCGC
    CAAATGCAAG ATGCTGCTGG CCGCCGCCAC TTCCATGCCT CTCAGTGCCC AAGGCCCACG
    AGTCCTGTGT CCACAGACAG CAACATGAGT GCTGTTGTGA TCCAGAAAGC CAGACCCGCC
    AAGAAGCAGA AACACCAGCC AGGACATCTG CGCAGGGAAG CCTACGCAGA TGATCTTCCA
    CCCCCTCCAG TGCCACCACC TGCTATAAAA TCGCCCACTG TCCAGTCCAA GGCACAGCTG
    GAGGTACGGC CTGTCATGGT GCCAAAACTC GCGTCTATAG AAGCAAGGAC AGATAGATCG
    TCAGACAGAA AAGGAGGCAG TTACAAGGGG AGAGAAGCTC TGGATGGAAG ACAAGTCACT
    GACCTGCGAA CAAATCCAAG TGACCCCAGA GAGGCACAGG AACAGCCAAA TGACGGGAAA
    GGCCGAGGAA CAAAGGCAGC CAAGCGAGAC CTTCCACCAG CAAAGACGCA CCTCGGCCAA
    GAGGATATTC TTCCGTACTG TAGACCTACT TTCCCAACGT CAAACAACCC TAGAGACCCC
    AGTTCCTCCA GCTCTATGTC ATCAAGAGGA TCAGGGAGCC GACAAAGAGA GCAAGCAAAT
    GTGGGCCGAA GGAATATGGC AGAAATGCAA GTACTTGGGG GATTTGAAAG AGGAGATGAG
    AATAATGAAG AATTAGAGGA AACTGAAAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52704
    Clone ID Related Accession (Same CDS sequence) XM_006522964.4 , XM_006522964.3
    Accession Version XM_006522964.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG
    CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT
    AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA
    GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA
    AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG
    TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG
    GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT
    CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA
    AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT
    GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT
    TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA
    CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT
    CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA
    GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA
    CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC
    AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT
    GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC
    CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC
    AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA
    GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT
    GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG
    CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT
    TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT
    GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC
    AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT
    AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA
    TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA
    GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523027.1
    CDS98..4969
    Translation

    Target ORF information:

    RefSeq Version XM_006522964.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522964.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCTGA GACCTGCACG GGGCTCAGTG TGCTGCAGGA AGGGCAAGGA AGACGTGTGC 
    TGTGTGTTGT CCTTGTGTGT TTTTCTGTCA CATGTGGATT TACTTGTGTC TGGACATGCT
    GTGATCGAAA TGGCAACTAC TGTTACTTCT GTGACCCCAT CAGCAGCTCT GGTGTTGTTT
    CCGACAGACC CTGAGGACCT AGAGAGGGGG AACGACAACG GAACGCCAGC CCCCACCTCT
    GATAACGATG ACAATTCGCT GGGCTACACA GGCTCCCGTC TTCGTCAGGA AGATTTTCCA
    CCCCGCATTG TTGAACACCC TTCAGACCTG ATCGTCTCCA AAGGAGAACC TGCCACTCTG
    AACTGTAAAG CCGAAGGCCG CCCCACCCCG ACGATAGAAT GGTACAAAGG TGGGGAAAGA
    GTGGAGACGG ACAAAGATGA CCCGCGCTCA CACCGGATGC TGCTGCCAAG CGGGTCTTTA
    TTTTTCTTAC GCATAGTCCA TGGACGGAAG AGTAGGCCCG ACGAAGGGGT CTACATCTGT
    GTGGCTAGGA ATTACCTCGG AGAGGCTGTG AGCCACAACG CATCGCTGGA GGTAGCTATA
    CTACGGGATG ACTTCAGACA AAACCCTTCA GATGTCATGG TGGCAGTAGG CGAACCTGCA
    GTCATGGAAT GCCAGCCTCC ACGGGGCCAT CCAGAGCCCA CCATCTCCTG GAAAAAGGAT
    GGCTCACCCC TGGATGATAA AGATGAAAGA ATCACTATAC GAGGAGGAAA ACTCATGATC
    ACTTATACGC GTAAAAGCGA TGCTGGCAAG TATGTTTGCG TTGGCACCAA CATGGTGGGG
    GAACGGGAGA GTGAAGTCGC AGAGCTAACC GTTTTAGAGA GACCATCGTT TGTGAAGAGA
    CCCAGTAATT TGGCAGTGAC TGTGGATGAC AGTGCAGAAT TTAAATGTGA GGCCCGAGGA
    GACCCTGTGC CTACAGTTCG ATGGAGGAAA GATGACGGAG AGCTGCCCAA ATCCAGATAT
    GAAATCCGAG ATGATCATAC TTTGAAAATT CGGAAGGTGA CTGCTGGCGA CATGGGATCA
    TACACTTGTG TGGCAGAAAA TATGGTCGGG AAAGCTGAAG CTTCCGCTAC TTTGACAGTT
    CAAGTTGGGT CTGAGCCGCC ACATTTCGTT GTAAAACCTC GGGACCAGGT GGTTGCCTTG
    GGAAGAACTG TGACGTTTCA GTGCGAAGCA ACTGGAAATC CTCAACCAGC TATCTTTTGG
    AGGAGAGAGG GGAGTCAGAA TCTACTTTTC TCCTACCAGC CTCCACAGTC CTCTAGCCGA
    TTTTCAGTTT CGCAGACAGG AGACCTCACC ATTACGAACG TCCAGCGGTC TGATGTCGGC
    TACTACATCT GTCAGACTTT AAATGTTGCT GGAAGTATCA TCACAAAGGC CTATTTGGAA
    GTTACTGATG TGATTGCAGA CCGGCCTCCC CCAGTCATTC GGCAAGGTCC TGTGAATCAG
    ACTGTAGCAG TGGATGGCAC TTTAATCCTC AGCTGTGTGG CCACAGGCAG TCCAGCACCC
    ACAATTCTGT GGAGGAAGGA TGGAGTCCTT GTTTCAACCC AAGATTCTCG GATCAAACAG
    CTGGAGAGTG GCGTGCTGCA GATCCGCTAC GCTAAGCTGG GTGACACGGG TCGGTACACC
    TGCACCGCAT CGACCCCCAG CGGTGAGGCC ACGTGGAGCG CTTACATCGA AGTCCAGGAA
    TTTGGGGTTC CAGTTCAACC CCCAAGACCC ACCGACCCCA ACTTAATCCC CAGCGCCCCC
    TCAAAGCCCG AAGTTACAGA TGTCAGCAAA AACACGGTCA CTTTATCGTG GCAGCCAAAC
    TTGAACTCAG GAGCAACCCC AACATCTTAT ATTATAGAAG CCTTCAGCCA TGCATCTGGT
    AGCAGCTGGC AGACGGCGGC AGAAAATGTG AAAACAGAAA CATTTGCCAT CAAAGGACTC
    AAACCTAACG CCATTTACCT TTTCCTGGTG AGGGCAGCAA ATGCCTATGG AATTAGTGAT
    CCAAGCCAAA TATCAGATCC AGTGAAAACG CAAGATGTCC CACCAACCAG CCAAGGGGTG
    GACCACAAGC AGGTCCAAAG AGAACTGGGG AATGTTGTCT TACATCTGCA CAACCCGACC
    ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC TGGACGGTGG ACCAACAATC TCAATATATT
    CAAGGCTATA AAATTCTCTA CCGCCCATCT GGAGCCAGCC ATGGTGAATC GGAGTGGTTA
    GTTTTTGAAG TGAGGACGCC AACGAAAAAC AGCGTGGTGA TCCCCGATCT CAGAAAAGGC
    GTCAACTACG AGATTAAGGC TCGCCCATTT TTTAATGAGT TTCAAGGAGC AGACAGTGAA
    ATCAAATTTG CCAAAACCTT AGAAGAAGCA CCAAGTGCCC CTCCCCGAAG TGTAACTGTA
    TCCAAGAATG ATGGAAATGG GACTGCAATC CTGGTCACTT GGCAACCGCC TCCTGAAGAC
    ACACAAAATG GAATGGTCCA AGAATATAAG GTTTGGTGTC TCGGTAATGA AACGAAGTAT
    CACATAAACA AGACAGTCGA TGGCTCGACC TTCTCGGTGG TCATCCCCTC TCTGGTTCCT
    GGGATCCGAT ACAGTGTGGA GGTGGCAGCG AGCACTGGGG CGGGTCCCGG GGTGAAGAGC
    GAACCTCAGT TCATCCAGTT AGATTCTCAC GGAAACCCCG TGTCTCCCGA GGACCAAGTC
    AGCCTTGCTC AGCAGATCTC CGACGTGGTG AGGCAGCCGG CGTTCATTGC AGGCATTGGG
    GCAGCCTGTT GGATTATCCT CATGGTCTTC AGCATCTGGC TTTACAGACA CCGCAAGAAG
    AGAAACGGAC TCACCAGCAC GTATGCGGGT ATCCGGAAAG TAACTTATCA AAGAGGAGGC
    GAAGCCGTCA GCAGTGGAGG GAGGCCGGGA CTCCTCAACA TTAGTGAACC TGCCACGCAG
    CCATGGCTGG CAGACACGTG GCCCAACACA GGCAACAACC ACAATGACTG CTCCATCAAC
    TGCTGCACGG CCGGCAATGG CAACAGCGAC AGCAACCTGA CAACCTACAG TCGCCCAGGG
    CAGCCCACTC CCTATGCCAC CACTCAACTC ATCCAGGCGA ACCTCAGCAA CAATATGAAT
    AATGGCGCCG GGGACTCCAG CGAGAAGCAC TGGAAGCCTC CGGGACAGCA GAAACCGGAA
    GTGGCACCAA TTCAGTATAA CATCATGGAG CAAAACAAGC TGAACAAAGA TTATCGGGCA
    AATGACACGA TCCCTCCAAC CATCCCATAC AACCAGTCCT ATGACCAGAA CACAGGAGGG
    TCTTATAACA GCTCAGATCG GGGCAGTAGT ACATCCGGGA GTCAAGGCCA CAAGAAAGGG
    GCACGGACAC CAAAGGCACC CAAGCAGGGT GGCATGAACT GGGCAGACTT GCTGCCCCCT
    CCTCCGGCGC ATCCTCCCCC ACATAGCAAC AGTGAAGAAT ACAACATGTC CGTAGATGAA
    AGCTATGATC AAGAAATGCC CTGTCCAGTG CCACCTGCGC GGATGTATTT GCAACAGGAT
    GAATTAGAGG AAGAGGAAGA TGAAAGAGGC CCCACTCCCC CTGTCCGGGG GGCAGCTTCT
    TCTCCAGCTG CTGTGTCCTA CAGCCACCAA TCCACGGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAGCTCC AGCCCATGTT ACAGGATTGT CCGGAGGACC TCGGGCACAT GCCCCACCCA
    CCAGACAGGA GGCGGCAGCC CGTGAGTCCT CCTCCACCGC CACGGCCAAT TTCCCCACCT
    CATACTTACG GCTACATTTC AGGACCCCTT GTCTCAGATA TGGATACCGA TGCCCCAGAA
    GAGGAGGAGG ACGAAGCTGA CATGGAAGTT GCCAAAATGC AGACCCGAAG GCTGTTGTTA
    CGTGGGCTGG AGCAGACCCC AGCATCCAGT GTTGGGGACC TTGAGAGCTC CGTCACTGGG
    TCCATGATCA ATGGCTGGGG CTCAGCCTCG GAAGAGGACA ACATTTCCAG TGGGCGCTCC
    AGTGTCAGTT CATCCGATGG CTCCTTTTTC ACTGACGCTG ATTTTGCCCA GGCAGTTGCT
    GCAGCTGCGG AGTATGCGGG CCTGAAAGTG GCTCGCCGCC AAATGCAAGA TGCTGCTGGC
    CGCCGCCACT TCCATGCCTC TCAGTGCCCA AGGCCCACGA GTCCTGTGTC CACAGACAGC
    AACATGAGTG CTGTTGTGAT CCAGAAAGCC AGACCCGCCA AGAAGCAGAA ACACCAGCCA
    GGACATCTGC GCAGGGAAGC CTACGCAGAT GATCTTCCAC CCCCTCCAGT GCCACCACCT
    GCTATAAAAT CGCCCACTGT CCAGTCCAAG GCACAGCTGG AGGTACGGCC TGTCATGGTG
    CCAAAACTCG CGTCTATAGA AGCAAGGACA GATAGATCGT CAGACAGAAA AGGAGGCAGT
    TACAAGGGGA GAGAAGCTCT GGATGGAAGA CAAGTCACTG ACCTGCGAAC AAATCCAAGT
    GACCCCAGAG AGGCACAGGA ACAGCCAAAT GACGGGAAAG GCCGAGGAAC AAAGGCAGCC
    AAGCGAGACC TTCCACCAGC AAAGACGCAC CTCGGCCAAG AGGATATTCT TCCGTACTGT
    AGACCTACTT TCCCAACGTC AAACAACCCT AGAGACCCCA GTTCCTCCAG CTCTATGTCA
    TCAAGAGGAT CAGGGAGCCG ACAAAGAGAG CAAGCAAATG TGGGCCGAAG GAATATGGCA
    GAAATGCAAG TACTTGGGGG ATTTGAAAGA GGAGATGAGA ATAATGAAGA ATTAGAGGAA
    ACTGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu52705
    Clone ID Related Accession (Same CDS sequence) XM_006522965.1 , XM_006522965.2
    Accession Version XM_006522965.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA
    CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC
    CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG
    AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT
    ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT
    GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC
    CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG
    GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA
    GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT
    GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT
    GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC
    AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG
    GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT
    GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT
    GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG
    CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG
    AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG
    GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG
    GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA
    GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT
    CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG
    AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC
    CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC
    AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT
    AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT
    CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA
    AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC
    CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG
    GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG
    AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG
    GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA
    TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG
    AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG
    TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG
    AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC
    GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG
    TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA
    AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT
    GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG
    CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG
    ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC
    AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA
    AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006523028.1
    CDS1107..5954
    Translation

    Target ORF information:

    RefSeq Version XM_006522965.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006522965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATCGCGG AGCCTGCTCG CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
    CGTCTTCGTC AGGAAGATTT TCCACCCCGC ATTGTTGAAC ACCCTTCAGA CCTGATCGTC
    TCCAAAGGAG AACCTGCCAC TCTGAACTGT AAAGCCGAAG GCCGCCCCAC CCCGACGATA
    GAATGGTACA AAGGTGGGGA AAGAGTGGAG ACGGACAAAG ATGACCCGCG CTCACACCGG
    ATGCTGCTGC CAAGCGGGTC TTTATTTTTC TTACGCATAG TCCATGGACG GAAGAGTAGG
    CCCGACGAAG GGGTCTACAT CTGTGTGGCT AGGAATTACC TCGGAGAGGC TGTGAGCCAC
    AACGCATCGC TGGAGGTAGC TATACTACGG GATGACTTCA GACAAAACCC TTCAGATGTC
    ATGGTGGCAG TAGGCGAACC TGCAGTCATG GAATGCCAGC CTCCACGGGG CCATCCAGAG
    CCCACCATCT CCTGGAAAAA GGATGGCTCA CCCCTGGATG ATAAAGATGA AAGAATCACT
    ATACGAGGAG GAAAACTCAT GATCACTTAT ACGCGTAAAA GCGATGCTGG CAAGTATGTT
    TGCGTTGGCA CCAACATGGT GGGGGAACGG GAGAGTGAAG TCGCAGAGCT AACCGTTTTA
    GAGAGACCAT CGTTTGTGAA GAGACCCAGT AATTTGGCAG TGACTGTGGA TGACAGTGCA
    GAATTTAAAT GTGAGGCCCG AGGAGACCCT GTGCCTACAG TTCGATGGAG GAAAGATGAC
    GGAGAGCTGC CCAAATCCAG ATATGAAATC CGAGATGATC ATACTTTGAA AATTCGGAAG
    GTGACTGCTG GCGACATGGG ATCATACACT TGTGTGGCAG AAAATATGGT CGGGAAAGCT
    GAAGCTTCCG CTACTTTGAC AGTTCAAGTT GGGTCTGAGC CGCCACATTT CGTTGTAAAA
    CCTCGGGACC AGGTGGTTGC CTTGGGAAGA ACTGTGACGT TTCAGTGCGA AGCAACTGGA
    AATCCTCAAC CAGCTATCTT TTGGAGGAGA GAGGGGAGTC AGAATCTACT TTTCTCCTAC
    CAGCCTCCAC AGTCCTCTAG CCGATTTTCA GTTTCGCAGA CAGGAGACCT CACCATTACG
    AACGTCCAGC GGTCTGATGT CGGCTACTAC ATCTGTCAGA CTTTAAATGT TGCTGGAAGT
    ATCATCACAA AGGCCTATTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCAGTC
    ATTCGGCAAG GTCCTGTGAA TCAGACTGTA GCAGTGGATG GCACTTTAAT CCTCAGCTGT
    GTGGCCACAG GCAGTCCAGC ACCCACAATT CTGTGGAGGA AGGATGGAGT CCTTGTTTCA
    ACCCAAGATT CTCGGATCAA ACAGCTGGAG AGTGGCGTGC TGCAGATCCG CTACGCTAAG
    CTGGGTGACA CGGGTCGGTA CACCTGCACC GCATCGACCC CCAGCGGTGA GGCCACGTGG
    AGCGCTTACA TCGAAGTCCA GGAATTTGGG GTTCCAGTTC AACCCCCAAG ACCCACCGAC
    CCCAACTTAA TCCCCAGCGC CCCCTCAAAG CCCGAAGTTA CAGATGTCAG CAAAAACACG
    GTCACTTTAT CGTGGCAGCC AAACTTGAAC TCAGGAGCAA CCCCAACATC TTATATTATA
    GAAGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAGACGG CGGCAGAAAA TGTGAAAACA
    GAAACATTTG CCATCAAAGG ACTCAAACCT AACGCCATTT ACCTTTTCCT GGTGAGGGCA
    GCAAATGCCT ATGGAATTAG TGATCCAAGC CAAATATCAG ATCCAGTGAA AACGCAAGAT
    GTCCCACCAA CCAGCCAAGG GGTGGACCAC AAGCAGGTCC AAAGAGAACT GGGGAATGTT
    GTCTTACATC TGCACAACCC GACCATCCTT TCTTCCTCCT CAGTCGAAGT GCACTGGACG
    GTGGACCAAC AATCTCAATA TATTCAAGGC TATAAAATTC TCTACCGCCC ATCTGGAGCC
    AGCCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CGCCAACGAA AAACAGCGTG
    GTGATCCCCG ATCTCAGAAA AGGCGTCAAC TACGAGATTA AGGCTCGCCC ATTTTTTAAT
    GAGTTTCAAG GAGCAGACAG TGAAATCAAA TTTGCCAAAA CCTTAGAAGA AGCACCAAGT
    GCCCCTCCCC GAAGTGTAAC TGTATCCAAG AATGATGGAA ATGGGACTGC AATCCTGGTC
    ACTTGGCAAC CGCCTCCTGA AGACACACAA AATGGAATGG TCCAAGAATA TAAGGTTTGG
    TGTCTCGGTA ATGAAACGAA GTATCACATA AACAAGACAG TCGATGGCTC GACCTTCTCG
    GTGGTCATCC CCTCTCTGGT TCCTGGGATC CGATACAGTG TGGAGGTGGC AGCGAGCACT
    GGGGCGGGTC CCGGGGTGAA GAGCGAACCT CAGTTCATCC AGTTAGATTC TCACGGAAAC
    CCCGTGTCTC CCGAGGACCA AGTCAGCCTT GCTCAGCAGA TCTCCGACGT GGTGAGGCAG
    CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGCTTTACA GACACCGCAA GAAGAGAAAC GGACTCACCA GCACGTATGC GGGTATCCGG
    AAAGTCCCGT CCTTTACCTT CACGCCAACA GTAACTTATC AAAGAGGAGG CGAAGCCGTC
    AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC ATTAGTGAAC CTGCCACGCA GCCATGGCTG
    GCAGACACGT GGCCCAACAC AGGCAACAAC CACAATGACT GCTCCATCAA CTGCTGCACG
    GCCGGCAATG GCAACAGCGA CAGCAACCTG ACAACCTACA GTCGCCCAGC TGATTGTATA
    GCCAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT
    GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA
    AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCCTA TGCCACCACT
    CAACTCATCC AGGCGAACCT CAGCAACAAT ATGAATAATG GCGCCGGGGA CTCCAGCGAG
    AAGCACTGGA AGCCTCCGGG ACAGCAGAAA CCGGAAGTGG CACCAATTCA GTATAACATC
    ATGGAGCAAA ACAAGCTGAA CAAAGATTAT CGGGCAAATG ACACGATCCC TCCAACCATC
    CCATACAACC AGTCCTATGA CCAGAACACA GGAGGGTCTT ATAACAGCTC AGATCGGGGC
    AGTAGTACAT CCGGGAGTCA AGGCCACAAG AAAGGGGCAC GGACACCAAA GGCACCCAAG
    CAGGGTGGCA TGAACTGGGC AGACTTGCTG CCCCCTCCTC CGGCGCATCC TCCCCCACAT
    AGCAACAGTG AAGAATACAA CATGTCCGTA GATGAAAGCT ATGATCAAGA AATGCCCTGT
    CCAGTGCCAC CTGCGCGGAT GTATTTGCAA CAGGATGAAT TAGAGGAAGA GGAAGATGAA
    AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA GCTTCTTCTC CAGCTGCTGT GTCCTACAGC
    CACCAATCCA CGGCCACTCT CACCCCTTCT CCCCAGGAAG AGCTCCAGCC CATGTTACAG
    GATTGTCCGG AGGACCTCGG GCACATGCCC CACCCACCAG ACAGGAGGCG GCAGCCCGTG
    AGTCCTCCTC CACCGCCACG GCCAATTTCC CCACCTCATA CTTACGGCTA CATTTCAGGA
    CCCCTTGTCT CAGATATGGA TACCGATGCC CCAGAAGAGG AGGAGGACGA AGCTGACATG
    GAAGTTGCCA AAATGCAGAC CCGAAGGCTG TTGTTACGTG GGCTGGAGCA GACCCCAGCA
    TCCAGTGTTG GGGACCTTGA GAGCTCCGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA
    GCCTCGGAAG AGGACAACAT TTCCAGTGGG CGCTCCAGTG TCAGTTCATC CGATGGCTCC
    TTTTTCACTG ACGCTGATTT TGCCCAGGCA GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG
    AAAGTGGCTC GCCGCCAAAT GCAAGATGCT GCTGGCCGCC GCCACTTCCA TGCCTCTCAG
    TGCCCAAGGC CCACGAGTCC TGTGTCCACA GACAGCAACA TGAGTGCTGT TGTGATCCAG
    AAAGCCAGAC CCGCCAAGAA GCAGAAACAC CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC
    GCAGATGATC TTCCACCCCC TCCAGTGCCA CCACCTGCTA TAAAATCGCC CACTGTCCAG
    TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC ATGGTGCCAA AACTCGCGTC TATAGAAGCA
    AGGACAGATA GATCGTCAGA CAGAAAAGGA GGCAGTTACA AGGGGAGAGA AGCTCTGGAT
    GGAAGACAAG TCACTGACCT GCGAACAAAT CCAAGTGACC CCAGAGAGGC ACAGGAACAG
    CCAAATGACG GGAAAGGCCG AGGAACAAAG GCAGCCAAGC GAGACCTTCC ACCAGCAAAG
    ACGCACCTCG GCCAAGAGGA TATTCTTCCG TACTGTAGAC CTACTTTCCC AACGTCAAAC
    AACCCTAGAG ACCCCAGTTC CTCCAGCTCT ATGTCATCAA GAGGATCAGG GAGCCGACAA
    AGAGAGCAAG CAAATGTGGG CCGAAGGAAT ATGGCAGAAA TGCAAGTACT TGGGGGATTT
    GAAAGAGGAG ATGAGAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68623
    Clone ID Related Accession (Same CDS sequence) XM_011246105.2
    Accession Version XM_011246105.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
    GACCCTGAGG ACCTAGAGAG GGGGAACGAC AACGGAACGC CAGCCCCCAC CTCTGATAAC
    GATGACAATT CGCTGGGCTA CACAGGCTCC CGTCTTCGTC AGGAAGATTT TCCACCCCGC
    ATTGTTGAAC ACCCTTCAGA CCTGATCGTC TCCAAAGGAG AACCTGCCAC TCTGAACTGT
    AAAGCCGAAG GCCGCCCCAC CCCGACGATA GAATGGTACA AAGGTGGGGA AAGAGTGGAG
    ACGGACAAAG ATGACCCGCG CTCACACCGG ATGCTGCTGC CAAGCGGGTC TTTATTTTTC
    TTACGCATAG TCCATGGACG GAAGAGTAGG CCCGACGAAG GGGTCTACAT CTGTGTGGCT
    AGGAATTACC TCGGAGAGGC TGTGAGCCAC AACGCATCGC TGGAGGTAGC TATACTACGG
    GATGACTTCA GACAAAACCC TTCAGATGTC ATGGTGGCAG TAGGCGAACC TGCAGTCATG
    GAATGCCAGC CTCCACGGGG CCATCCAGAG CCCACCATCT CCTGGAAAAA GGATGGCTCA
    CCCCTGGATG ATAAAGATGA AAGAATCACT ATACGAGGAG GAAAACTCAT GATCACTTAT
    ACGCGTAAAA GCGATGCTGG CAAGTATGTT TGCGTTGGCA CCAACATGGT GGGGGAACGG
    GAGAGTGAAG TCGCAGAGCT AACCGTTTTA GAGAGACCAT CGTTTGTGAA GAGACCCAGT
    AATTTGGCAG TGACTGTGGA TGACAGTGCA GAATTTAAAT GTGAGGCCCG AGGAGACCCT
    GTGCCTACAG TTCGATGGAG GAAAGATGAC GGAGAGCTGC CCAAATCCAG ATATGAAATC
    CGAGATGATC ATACTTTGAA AATTCGGAAG GTGACTGCTG GCGACATGGG ATCATACACT
    TGTGTGGCAG AAAATATGGT CGGGAAAGCT GAAGCTTCCG CTACTTTGAC AGTTCAAGTT
    GGGTCTGAGC CGCCACATTT CGTTGTAAAA CCTCGGGACC AGGTGGTTGC CTTGGGAAGA
    ACTGTGACGT TTCAGTGCGA AGCAACTGGA AATCCTCAAC CAGCTATCTT TTGGAGGAGA
    GAGGGGAGTC AGAATCTACT TTTCTCCTAC CAGCCTCCAC AGTCCTCTAG CCGATTTTCA
    GTTTCGCAGA CAGGAGACCT CACCATTACG AACGTCCAGC GGTCTGATGT CGGCTACTAC
    ATCTGTCAGA CTTTAAATGT TGCTGGAAGT ATCATCACAA AGGCCTATTT GGAAGTTACT
    GATGTGATTG CAGACCGGCC TCCCCCAGTC ATTCGGCAAG GTCCTGTGAA TCAGACTGTA
    GCAGTGGATG GCACTTTAAT CCTCAGCTGT GTGGCCACAG GCAGTCCAGC ACCCACAATT
    CTGTGGAGGA AGGATGGAGT CCTTGTTTCA ACCCAAGATT CTCGGATCAA ACAGCTGGAG
    AGTGGCGTGC TGCAGATCCG CTACGCTAAG CTGGGTGACA CGGGTCGGTA CACCTGCACC
    GCATCGACCC CCAGCGGTGA GGCCACGTGG AGCGCTTACA TCGAAGTCCA GGAATTTGGG
    GTTCCAGTTC AACCCCCAAG ACCCACCGAC CCCAACTTAA TCCCCAGCGC CCCCTCAAAG
    CCCGAAGTTA CAGATGTCAG CAAAAACACG GTCACTTTAT CGTGGCAGCC AAACTTGAAC
    TCAGGAGCAA CCCCAACATC TTATATTATA GAAGCCTTCA GCCATGCATC TGGTAGCAGC
    TGGCAGACGG CGGCAGAAAA TGTGAAAACA GAAACATTTG CCATCAAAGG ACTCAAACCT
    AACGCCATTT ACCTTTTCCT GGTGAGGGCA GCAAATGCCT ATGGAATTAG TGATCCAAGC
    CAAATATCAG ATCCAGTGAA AACGCAAGAT GTCCCACCAA CCAGCCAAGG GGTGGACCAC
    AAGCAGGTCC AAAGAGAACT GGGGAATGTT GTCTTACATC TGCACAACCC GACCATCCTT
    TCTTCCTCCT CAGTCGAAGT GCACTGGACG GTGGACCAAC AATCTCAATA TATTCAAGGC
    TATAAAATTC TCTACCGCCC ATCTGGAGCC AGCCATGGTG AATCGGAGTG GTTAGTTTTT
    GAAGTGAGGA CGCCAACGAA AAACAGCGTG GTGATCCCCG ATCTCAGAAA AGGCGTCAAC
    TACGAGATTA AGGCTCGCCC ATTTTTTAAT GAGTTTCAAG GAGCAGACAG TGAAATCAAA
    TTTGCCAAAA CCTTAGAAGA AGCACCAAGT GCCCCTCCCC GAAGTGTAAC TGTATCCAAG
    AATGATGGAA ATGGGACTGC AATCCTGGTC ACTTGGCAAC CGCCTCCTGA AGACACACAA
    AATGGAATGG TCCAAGAATA TAAGGTTTGG TGTCTCGGTA ATGAAACGAA GTATCACATA
    AACAAGACAG TCGATGGCTC GACCTTCTCG GTGGTCATCC CCTCTCTGGT TCCTGGGATC
    CGATACAGTG TGGAGGTGGC AGCGAGCACT GGGGCGGGTC CCGGGGTGAA GAGCGAACCT
    CAGTTCATCC AGTTAGATTC TCACGGAAAC CCCGTGTCTC CCGAGGACCA AGTCAGCCTT
    GCTCAGCAGA TCTCCGACGT GGTGAGGCAG CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC
    TGTTGGATTA TCCTCATGGT CTTCAGCATC TGGCTTTACA GACACCGCAA GAAGAGAAAC
    GGACTCACCA GCACGTATGC GGGTATCCGG AAAGTCCCGT CCTTTACCTT CACGCCAACA
    GTAACTTATC AAAGAGGAGG CGAAGCCGTC AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC
    ATTAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCCAACAC AGGCAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACG GCCGGCAATG GCAACAGCGA CAGCAACCTG
    ACAACCTACA GTCGCCCAGC TGATTGTATA GCCAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ACGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACTCCCTA TGCCACCACT CAACTCATCC AGGCGAACCT CAGCAACAAT
    ATGAATAATG GCGCCGGGGA CTCCAGCGAG AAGCACTGGA AGCCTCCGGG ACAGCAGAAA
    CCGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAGCTGAA CAAAGATTAT
    CGGGCAAATG ACACGATCCC TCCAACCATC CCATACAACC AGTCCTATGA CCAGAACACA
    GGAGGGTCTT ATAACAGCTC AGATCGGGGC AGTAGTACAT CCGGGAGTCA AGGCCACAAG
    AAAGGGGCAC GGACACCAAA GGCACCCAAG CAGGGTGGCA TGAACTGGGC AGACTTGCTG
    CCCCCTCCTC CGGCGCATCC TCCCCCACAT AGCAACAGTG AAGAATACAA CATGTCCGTA
    GATGAAAGCT ATGATCAAGA AATGCCCTGT CCAGTGCCAC CTGCGCGGAT GTATTTGCAA
    CAGGATGAAT TAGAGGAAGA GGAAGATGAA AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA
    GCTTCTTCTC CAGCTGCTGT GTCCTACAGC CACCAATCCA CGGCCACTCT CACCCCTTCT
    CCCCAGGAAG AGCTCCAGCC CATGTTACAG GATTGTCCGG AGGACCTCGG GCACATGCCC
    CACCCACCAG ACAGGAGGCG GCAGCCCGTG AGTCCTCCTC CACCGCCACG GCCAATTTCC
    CCACCTCATA CTTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACCGATGCC
    CCAGAAGAGG AGGAGGACGA AGCTGACATG GAAGTTGCCA AAATGCAGAC CCGAAGGCTG
    TTGTTACGTG GGCTGGAGCA GACCCCAGCA TCCAGTGTTG GGGACCTTGA GAGCTCCGTC
    ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCGGAAG AGGACAACAT TTCCAGTGGG
    CGCTCCAGTG TCAGTTCATC CGATGGCTCC TTTTTCACTG ACGCTGATTT TGCCCAGGCA
    GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG AAAGTGGCTC GCCGCCAAAT GCAAGATGCT
    GCTGGCCGCC GCCACTTCCA TGCCTCTCAG TGCCCAAGGC CCACGAGTCC TGTGTCCACA
    GACAGCAACA TGAGTGCTGT TGTGATCCAG AAAGCCAGAC CCGCCAAGAA GCAGAAACAC
    CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC GCAGATGATC TTCCACCCCC TCCAGTGCCA
    CCACCTGCTA TAAAATCGCC CACTGTCCAG TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC
    ATGGTGCCAA AACTCGCGTC TATAGAAGCA AGGACAGATA GATCGTCAGA CAGAAAAGGA
    GGCAGTTACA AGGGGAGAGA AGCTCTGGAT GGAAGACAAG TCACTGACCT GCGAACAAAT
    CCAAGTGACC CCAGAGAGGC ACAGGAACAG CCAAATGACG GGAAAGGCCG AGGAACAAAG
    GCAGCCAAGC GAGACCTTCC ACCAGCAAAG ACGCACCTCG GCCAAGAGGA TATTCTTCCG
    TACTGTAGAC CTACTTTCCC AACGTCAAAC AACCCTAGAG ACCCCAGTTC CTCCAGCTCT
    ATGTCATCAA GAGGATCAGG GAGCCGACAA AGAGAGCAAG CAAATGTGGG CCGAAGGAAT
    ATGGCAGAAA TGCAAGTACT TGGGGGATTT GAAAGAGGAG ATGAGAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244407.1
    CDS20..4957
    Translation

    Target ORF information:

    RefSeq Version XM_011246105.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246105.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
    GACCCTGAGG ACCTAGAGAG GGGGAACGAC AACGGAACGC CAGCCCCCAC CTCTGATAAC
    GATGACAATT CGCTGGGCTA CACAGGCTCC CGTCTTCGTC AGGAAGATTT TCCACCCCGC
    ATTGTTGAAC ACCCTTCAGA CCTGATCGTC TCCAAAGGAG AACCTGCCAC TCTGAACTGT
    AAAGCCGAAG GCCGCCCCAC CCCGACGATA GAATGGTACA AAGGTGGGGA AAGAGTGGAG
    ACGGACAAAG ATGACCCGCG CTCACACCGG ATGCTGCTGC CAAGCGGGTC TTTATTTTTC
    TTACGCATAG TCCATGGACG GAAGAGTAGG CCCGACGAAG GGGTCTACAT CTGTGTGGCT
    AGGAATTACC TCGGAGAGGC TGTGAGCCAC AACGCATCGC TGGAGGTAGC TATACTACGG
    GATGACTTCA GACAAAACCC TTCAGATGTC ATGGTGGCAG TAGGCGAACC TGCAGTCATG
    GAATGCCAGC CTCCACGGGG CCATCCAGAG CCCACCATCT CCTGGAAAAA GGATGGCTCA
    CCCCTGGATG ATAAAGATGA AAGAATCACT ATACGAGGAG GAAAACTCAT GATCACTTAT
    ACGCGTAAAA GCGATGCTGG CAAGTATGTT TGCGTTGGCA CCAACATGGT GGGGGAACGG
    GAGAGTGAAG TCGCAGAGCT AACCGTTTTA GAGAGACCAT CGTTTGTGAA GAGACCCAGT
    AATTTGGCAG TGACTGTGGA TGACAGTGCA GAATTTAAAT GTGAGGCCCG AGGAGACCCT
    GTGCCTACAG TTCGATGGAG GAAAGATGAC GGAGAGCTGC CCAAATCCAG ATATGAAATC
    CGAGATGATC ATACTTTGAA AATTCGGAAG GTGACTGCTG GCGACATGGG ATCATACACT
    TGTGTGGCAG AAAATATGGT CGGGAAAGCT GAAGCTTCCG CTACTTTGAC AGTTCAAGTT
    GGGTCTGAGC CGCCACATTT CGTTGTAAAA CCTCGGGACC AGGTGGTTGC CTTGGGAAGA
    ACTGTGACGT TTCAGTGCGA AGCAACTGGA AATCCTCAAC CAGCTATCTT TTGGAGGAGA
    GAGGGGAGTC AGAATCTACT TTTCTCCTAC CAGCCTCCAC AGTCCTCTAG CCGATTTTCA
    GTTTCGCAGA CAGGAGACCT CACCATTACG AACGTCCAGC GGTCTGATGT CGGCTACTAC
    ATCTGTCAGA CTTTAAATGT TGCTGGAAGT ATCATCACAA AGGCCTATTT GGAAGTTACT
    GATGTGATTG CAGACCGGCC TCCCCCAGTC ATTCGGCAAG GTCCTGTGAA TCAGACTGTA
    GCAGTGGATG GCACTTTAAT CCTCAGCTGT GTGGCCACAG GCAGTCCAGC ACCCACAATT
    CTGTGGAGGA AGGATGGAGT CCTTGTTTCA ACCCAAGATT CTCGGATCAA ACAGCTGGAG
    AGTGGCGTGC TGCAGATCCG CTACGCTAAG CTGGGTGACA CGGGTCGGTA CACCTGCACC
    GCATCGACCC CCAGCGGTGA GGCCACGTGG AGCGCTTACA TCGAAGTCCA GGAATTTGGG
    GTTCCAGTTC AACCCCCAAG ACCCACCGAC CCCAACTTAA TCCCCAGCGC CCCCTCAAAG
    CCCGAAGTTA CAGATGTCAG CAAAAACACG GTCACTTTAT CGTGGCAGCC AAACTTGAAC
    TCAGGAGCAA CCCCAACATC TTATATTATA GAAGCCTTCA GCCATGCATC TGGTAGCAGC
    TGGCAGACGG CGGCAGAAAA TGTGAAAACA GAAACATTTG CCATCAAAGG ACTCAAACCT
    AACGCCATTT ACCTTTTCCT GGTGAGGGCA GCAAATGCCT ATGGAATTAG TGATCCAAGC
    CAAATATCAG ATCCAGTGAA AACGCAAGAT GTCCCACCAA CCAGCCAAGG GGTGGACCAC
    AAGCAGGTCC AAAGAGAACT GGGGAATGTT GTCTTACATC TGCACAACCC GACCATCCTT
    TCTTCCTCCT CAGTCGAAGT GCACTGGACG GTGGACCAAC AATCTCAATA TATTCAAGGC
    TATAAAATTC TCTACCGCCC ATCTGGAGCC AGCCATGGTG AATCGGAGTG GTTAGTTTTT
    GAAGTGAGGA CGCCAACGAA AAACAGCGTG GTGATCCCCG ATCTCAGAAA AGGCGTCAAC
    TACGAGATTA AGGCTCGCCC ATTTTTTAAT GAGTTTCAAG GAGCAGACAG TGAAATCAAA
    TTTGCCAAAA CCTTAGAAGA AGCACCAAGT GCCCCTCCCC GAAGTGTAAC TGTATCCAAG
    AATGATGGAA ATGGGACTGC AATCCTGGTC ACTTGGCAAC CGCCTCCTGA AGACACACAA
    AATGGAATGG TCCAAGAATA TAAGGTTTGG TGTCTCGGTA ATGAAACGAA GTATCACATA
    AACAAGACAG TCGATGGCTC GACCTTCTCG GTGGTCATCC CCTCTCTGGT TCCTGGGATC
    CGATACAGTG TGGAGGTGGC AGCGAGCACT GGGGCGGGTC CCGGGGTGAA GAGCGAACCT
    CAGTTCATCC AGTTAGATTC TCACGGAAAC CCCGTGTCTC CCGAGGACCA AGTCAGCCTT
    GCTCAGCAGA TCTCCGACGT GGTGAGGCAG CCGGCGTTCA TTGCAGGCAT TGGGGCAGCC
    TGTTGGATTA TCCTCATGGT CTTCAGCATC TGGCTTTACA GACACCGCAA GAAGAGAAAC
    GGACTCACCA GCACGTATGC GGGTATCCGG AAAGTCCCGT CCTTTACCTT CACGCCAACA
    GTAACTTATC AAAGAGGAGG CGAAGCCGTC AGCAGTGGAG GGAGGCCGGG ACTCCTCAAC
    ATTAGTGAAC CTGCCACGCA GCCATGGCTG GCAGACACGT GGCCCAACAC AGGCAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACG GCCGGCAATG GCAACAGCGA CAGCAACCTG
    ACAACCTACA GTCGCCCAGC TGATTGTATA GCCAATTATA ACAACCAACT GGATAACAAA
    CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ACGGGCGTTT TGTCAATCCA
    TCAGGGCAGC CCACTCCCTA TGCCACCACT CAACTCATCC AGGCGAACCT CAGCAACAAT
    ATGAATAATG GCGCCGGGGA CTCCAGCGAG AAGCACTGGA AGCCTCCGGG ACAGCAGAAA
    CCGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAGCTGAA CAAAGATTAT
    CGGGCAAATG ACACGATCCC TCCAACCATC CCATACAACC AGTCCTATGA CCAGAACACA
    GGAGGGTCTT ATAACAGCTC AGATCGGGGC AGTAGTACAT CCGGGAGTCA AGGCCACAAG
    AAAGGGGCAC GGACACCAAA GGCACCCAAG CAGGGTGGCA TGAACTGGGC AGACTTGCTG
    CCCCCTCCTC CGGCGCATCC TCCCCCACAT AGCAACAGTG AAGAATACAA CATGTCCGTA
    GATGAAAGCT ATGATCAAGA AATGCCCTGT CCAGTGCCAC CTGCGCGGAT GTATTTGCAA
    CAGGATGAAT TAGAGGAAGA GGAAGATGAA AGAGGCCCCA CTCCCCCTGT CCGGGGGGCA
    GCTTCTTCTC CAGCTGCTGT GTCCTACAGC CACCAATCCA CGGCCACTCT CACCCCTTCT
    CCCCAGGAAG AGCTCCAGCC CATGTTACAG GATTGTCCGG AGGACCTCGG GCACATGCCC
    CACCCACCAG ACAGGAGGCG GCAGCCCGTG AGTCCTCCTC CACCGCCACG GCCAATTTCC
    CCACCTCATA CTTACGGCTA CATTTCAGGA CCCCTTGTCT CAGATATGGA TACCGATGCC
    CCAGAAGAGG AGGAGGACGA AGCTGACATG GAAGTTGCCA AAATGCAGAC CCGAAGGCTG
    TTGTTACGTG GGCTGGAGCA GACCCCAGCA TCCAGTGTTG GGGACCTTGA GAGCTCCGTC
    ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCGGAAG AGGACAACAT TTCCAGTGGG
    CGCTCCAGTG TCAGTTCATC CGATGGCTCC TTTTTCACTG ACGCTGATTT TGCCCAGGCA
    GTTGCTGCAG CTGCGGAGTA TGCGGGCCTG AAAGTGGCTC GCCGCCAAAT GCAAGATGCT
    GCTGGCCGCC GCCACTTCCA TGCCTCTCAG TGCCCAAGGC CCACGAGTCC TGTGTCCACA
    GACAGCAACA TGAGTGCTGT TGTGATCCAG AAAGCCAGAC CCGCCAAGAA GCAGAAACAC
    CAGCCAGGAC ATCTGCGCAG GGAAGCCTAC GCAGATGATC TTCCACCCCC TCCAGTGCCA
    CCACCTGCTA TAAAATCGCC CACTGTCCAG TCCAAGGCAC AGCTGGAGGT ACGGCCTGTC
    ATGGTGCCAA AACTCGCGTC TATAGAAGCA AGGACAGATA GATCGTCAGA CAGAAAAGGA
    GGCAGTTACA AGGGGAGAGA AGCTCTGGAT GGAAGACAAG TCACTGACCT GCGAACAAAT
    CCAAGTGACC CCAGAGAGGC ACAGGAACAG CCAAATGACG GGAAAGGCCG AGGAACAAAG
    GCAGCCAAGC GAGACCTTCC ACCAGCAAAG ACGCACCTCG GCCAAGAGGA TATTCTTCCG
    TACTGTAGAC CTACTTTCCC AACGTCAAAC AACCCTAGAG ACCCCAGTTC CTCCAGCTCT
    ATGTCATCAA GAGGATCAGG GAGCCGACAA AGAGAGCAAG CAAATGTGGG CCGAAGGAAT
    ATGGCAGAAA TGCAAGTACT TGGGGGATTT GAAAGAGGAG ATGAGAATAA TGAAGAATTA
    GAGGAAACTG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68624
    Clone ID Related Accession (Same CDS sequence) XM_011246106.2
    Accession Version XM_011246106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product roundabout homolog 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
    GGCTCCCGTC TTCGTCAGGA AGATTTTCCA CCCCGCATTG TTGAACACCC TTCAGACCTG
    ATCGTCTCCA AAGGAGAACC TGCCACTCTG AACTGTAAAG CCGAAGGCCG CCCCACCCCG
    ACGATAGAAT GGTACAAAGG TGGGGAAAGA GTGGAGACGG ACAAAGATGA CCCGCGCTCA
    CACCGGATGC TGCTGCCAAG CGGGTCTTTA TTTTTCTTAC GCATAGTCCA TGGACGGAAG
    AGTAGGCCCG ACGAAGGGGT CTACATCTGT GTGGCTAGGA ATTACCTCGG AGAGGCTGTG
    AGCCACAACG CATCGCTGGA GGTAGCTATA CTACGGGATG ACTTCAGACA AAACCCTTCA
    GATGTCATGG TGGCAGTAGG CGAACCTGCA GTCATGGAAT GCCAGCCTCC ACGGGGCCAT
    CCAGAGCCCA CCATCTCCTG GAAAAAGGAT GGCTCACCCC TGGATGATAA AGATGAAAGA
    ATCACTATAC GAGGAGGAAA ACTCATGATC ACTTATACGC GTAAAAGCGA TGCTGGCAAG
    TATGTTTGCG TTGGCACCAA CATGGTGGGG GAACGGGAGA GTGAAGTCGC AGAGCTAACC
    GTTTTAGAGA GACCATCGTT TGTGAAGAGA CCCAGTAATT TGGCAGTGAC TGTGGATGAC
    AGTGCAGAAT TTAAATGTGA GGCCCGAGGA GACCCTGTGC CTACAGTTCG ATGGAGGAAA
    GATGACGGAG AGCTGCCCAA ATCCAGATAT GAAATCCGAG ATGATCATAC TTTGAAAATT
    CGGAAGGTGA CTGCTGGCGA CATGGGATCA TACACTTGTG TGGCAGAAAA TATGGTCGGG
    AAAGCTGAAG CTTCCGCTAC TTTGACAGTT CAAGTTGGGT CTGAGCCGCC ACATTTCGTT
    GTAAAACCTC GGGACCAGGT GGTTGCCTTG GGAAGAACTG TGACGTTTCA GTGCGAAGCA
    ACTGGAAATC CTCAACCAGC TATCTTTTGG AGGAGAGAGG GGAGTCAGAA TCTACTTTTC
    TCCTACCAGC CTCCACAGTC CTCTAGCCGA TTTTCAGTTT CGCAGACAGG AGACCTCACC
    ATTACGAACG TCCAGCGGTC TGATGTCGGC TACTACATCT GTCAGACTTT AAATGTTGCT
    GGAAGTATCA TCACAAAGGC CTATTTGGAA GTTACTGATG TGATTGCAGA CCGGCCTCCC
    CCAGTCATTC GGCAAGGTCC TGTGAATCAG ACTGTAGCAG TGGATGGCAC TTTAATCCTC
    AGCTGTGTGG CCACAGGCAG TCCAGCACCC ACAATTCTGT GGAGGAAGGA TGGAGTCCTT
    GTTTCAACCC AAGATTCTCG GATCAAACAG CTGGAGAGTG GCGTGCTGCA GATCCGCTAC
    GCTAAGCTGG GTGACACGGG TCGGTACACC TGCACCGCAT CGACCCCCAG CGGTGAGGCC
    ACGTGGAGCG CTTACATCGA AGTCCAGGAA TTTGGGGTTC CAGTTCAACC CCCAAGACCC
    ACCGACCCCA ACTTAATCCC CAGCGCCCCC TCAAAGCCCG AAGTTACAGA TGTCAGCAAA
    AACACGGTCA CTTTATCGTG GCAGCCAAAC TTGAACTCAG GAGCAACCCC AACATCTTAT
    ATTATAGAAG CCTTCAGCCA TGCATCTGGT AGCAGCTGGC AGACGGCGGC AGAAAATGTG
    AAAACAGAAA CATTTGCCAT CAAAGGACTC AAACCTAACG CCATTTACCT TTTCCTGGTG
    AGGGCAGCAA ATGCCTATGG AATTAGTGAT CCAAGCCAAA TATCAGATCC AGTGAAAACG
    CAAGATGTCC CACCAACCAG CCAAGGGGTG GACCACAAGC AGGTCCAAAG AGAACTGGGG
    AATGTTGTCT TACATCTGCA CAACCCGACC ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC
    TGGACGGTGG ACCAACAATC TCAATATATT CAAGGCTATA AAATTCTCTA CCGCCCATCT
    GGAGCCAGCC ATGGTGAATC GGAGTGGTTA GTTTTTGAAG TGAGGACGCC AACGAAAAAC
    AGCGTGGTGA TCCCCGATCT CAGAAAAGGC GTCAACTACG AGATTAAGGC TCGCCCATTT
    TTTAATGAGT TTCAAGGAGC AGACAGTGAA ATCAAATTTG CCAAAACCTT AGAAGAAGCA
    CCAAGTGCCC CTCCCCGAAG TGTAACTGTA TCCAAGAATG ATGGAAATGG GACTGCAATC
    CTGGTCACTT GGCAACCGCC TCCTGAAGAC ACACAAAATG GAATGGTCCA AGAATATAAG
    GTTTGGTGTC TCGGTAATGA AACGAAGTAT CACATAAACA AGACAGTCGA TGGCTCGACC
    TTCTCGGTGG TCATCCCCTC TCTGGTTCCT GGGATCCGAT ACAGTGTGGA GGTGGCAGCG
    AGCACTGGGG CGGGTCCCGG GGTGAAGAGC GAACCTCAGT TCATCCAGTT AGATTCTCAC
    GGAAACCCCG TGTCTCCCGA GGACCAAGTC AGCCTTGCTC AGCAGATCTC CGACGTGGTG
    AGGCAGCCGG CGTTCATTGC AGGCATTGGG GCAGCCTGTT GGATTATCCT CATGGTCTTC
    AGCATCTGGC TTTACAGACA CCGCAAGAAG AGAAACGGAC TCACCAGCAC GTATGCGGGT
    ATCCGGAAAG TCCCGTCCTT TACCTTCACG CCAACAGTAA CTTATCAAAG AGGAGGCGAA
    GCCGTCAGCA GTGGAGGGAG GCCGGGACTC CTCAACATTA GTGAACCTGC CACGCAGCCA
    TGGCTGGCAG ACACGTGGCC CAACACAGGC AACAACCACA ATGACTGCTC CATCAACTGC
    TGCACGGCCG GCAATGGCAA CAGCGACAGC AACCTGACAA CCTACAGTCG CCCAGCTGAT
    TGTATAGCCA ATTATAACAA CCAACTGGAT AACAAACAAA CAAATCTGAT GCTCCCTGAG
    TCAACTGTTT ATGGTGATGT GGACCTTAGT AACAAAATCA ATGAGATGAA AACCTTCAAT
    AGCCCAAATC TGAAGGACGG GCGTTTTGTC AATCCATCAG GGCAGCCCAC TCCCTATGCC
    ACCACTCAAC TCATCCAGGC GAACCTCAGC AACAATATGA ATAATGGCGC CGGGGACTCC
    AGCGAGAAGC ACTGGAAGCC TCCGGGACAG CAGAAACCGG AAGTGGCACC AATTCAGTAT
    AACATCATGG AGCAAAACAA GCTGAACAAA GATTATCGGG CAAATGACAC GATCCCTCCA
    ACCATCCCAT ACAACCAGTC CTATGACCAG AACACAGGAG GGTCTTATAA CAGCTCAGAT
    CGGGGCAGTA GTACATCCGG GAGTCAAGGC CACAAGAAAG GGGCACGGAC ACCAAAGGCA
    CCCAAGCAGG GTGGCATGAA CTGGGCAGAC TTGCTGCCCC CTCCTCCGGC GCATCCTCCC
    CCACATAGCA ACAGTGAAGA ATACAACATG TCCGTAGATG AAAGCTATGA TCAAGAAATG
    CCCTGTCCAG TGCCACCTGC GCGGATGTAT TTGCAACAGG ATGAATTAGA GGAAGAGGAA
    GATGAAAGAG GCCCCACTCC CCCTGTCCGG GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC
    TACAGCCACC AATCCACGGC CACTCTCACC CCTTCTCCCC AGGAAGAGCT CCAGCCCATG
    TTACAGGATT GTCCGGAGGA CCTCGGGCAC ATGCCCCACC CACCAGACAG GAGGCGGCAG
    CCCGTGAGTC CTCCTCCACC GCCACGGCCA ATTTCCCCAC CTCATACTTA CGGCTACATT
    TCAGGACCCC TTGTCTCAGA TATGGATACC GATGCCCCAG AAGAGGAGGA GGACGAAGCT
    GACATGGAAG TTGCCAAAAT GCAGACCCGA AGGCTGTTGT TACGTGGGCT GGAGCAGACC
    CCAGCATCCA GTGTTGGGGA CCTTGAGAGC TCCGTCACTG GGTCCATGAT CAATGGCTGG
    GGCTCAGCCT CGGAAGAGGA CAACATTTCC AGTGGGCGCT CCAGTGTCAG TTCATCCGAT
    GGCTCCTTTT TCACTGACGC TGATTTTGCC CAGGCAGTTG CTGCAGCTGC GGAGTATGCG
    GGCCTGAAAG TGGCTCGCCG CCAAATGCAA GATGCTGCTG GCCGCCGCCA CTTCCATGCC
    TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG TCCACAGACA GCAACATGAG TGCTGTTGTG
    ATCCAGAAAG CCAGACCCGC CAAGAAGCAG AAACACCAGC CAGGACATCT GCGCAGGGAA
    GCCTACGCAG ATGATCTTCC ACCCCCTCCA GTGCCACCAC CTGCTATAAA ATCGCCCACT
    GTCCAGTCCA AGGCACAGCT GGAGGTACGG CCTGTCATGG TGCCAAAACT CGCGTCTATA
    GAAGCAAGGA CAGATAGATC GTCAGACAGA AAAGGAGGCA GTTACAAGGG GAGAGAAGCT
    CTGGATGGAA GACAAGTCAC TGACCTGCGA ACAAATCCAA GTGACCCCAG AGAGGCACAG
    GAACAGCCAA ATGACGGGAA AGGCCGAGGA ACAAAGGCAG CCAAGCGAGA CCTTCCACCA
    GCAAAGACGC ACCTCGGCCA AGAGGATATT CTTCCGTACT GTAGACCTAC TTTCCCAACG
    TCAAACAACC CTAGAGACCC CAGTTCCTCC AGCTCTATGT CATCAAGAGG ATCAGGGAGC
    CGACAAAGAG AGCAAGCAAA TGTGGGCCGA AGGAATATGG CAGAAATGCA AGTACTTGGG
    GGATTTGAAA GAGGAGATGA GAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244408.1
    CDS16..4869
    Translation

    Target ORF information:

    RefSeq Version XM_011246106.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus roundabout guidance receptor 1 (Robo1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011246106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAATCACA GCCGCTGTAG ACTCTTGCGT GCAAAGGGCC TGTCATGTCC AGAAGAAACT 
    GGCTCCCGTC TTCGTCAGGA AGATTTTCCA CCCCGCATTG TTGAACACCC TTCAGACCTG
    ATCGTCTCCA AAGGAGAACC TGCCACTCTG AACTGTAAAG CCGAAGGCCG CCCCACCCCG
    ACGATAGAAT GGTACAAAGG TGGGGAAAGA GTGGAGACGG ACAAAGATGA CCCGCGCTCA
    CACCGGATGC TGCTGCCAAG CGGGTCTTTA TTTTTCTTAC GCATAGTCCA TGGACGGAAG
    AGTAGGCCCG ACGAAGGGGT CTACATCTGT GTGGCTAGGA ATTACCTCGG AGAGGCTGTG
    AGCCACAACG CATCGCTGGA GGTAGCTATA CTACGGGATG ACTTCAGACA AAACCCTTCA
    GATGTCATGG TGGCAGTAGG CGAACCTGCA GTCATGGAAT GCCAGCCTCC ACGGGGCCAT
    CCAGAGCCCA CCATCTCCTG GAAAAAGGAT GGCTCACCCC TGGATGATAA AGATGAAAGA
    ATCACTATAC GAGGAGGAAA ACTCATGATC ACTTATACGC GTAAAAGCGA TGCTGGCAAG
    TATGTTTGCG TTGGCACCAA CATGGTGGGG GAACGGGAGA GTGAAGTCGC AGAGCTAACC
    GTTTTAGAGA GACCATCGTT TGTGAAGAGA CCCAGTAATT TGGCAGTGAC TGTGGATGAC
    AGTGCAGAAT TTAAATGTGA GGCCCGAGGA GACCCTGTGC CTACAGTTCG ATGGAGGAAA
    GATGACGGAG AGCTGCCCAA ATCCAGATAT GAAATCCGAG ATGATCATAC TTTGAAAATT
    CGGAAGGTGA CTGCTGGCGA CATGGGATCA TACACTTGTG TGGCAGAAAA TATGGTCGGG
    AAAGCTGAAG CTTCCGCTAC TTTGACAGTT CAAGTTGGGT CTGAGCCGCC ACATTTCGTT
    GTAAAACCTC GGGACCAGGT GGTTGCCTTG GGAAGAACTG TGACGTTTCA GTGCGAAGCA
    ACTGGAAATC CTCAACCAGC TATCTTTTGG AGGAGAGAGG GGAGTCAGAA TCTACTTTTC
    TCCTACCAGC CTCCACAGTC CTCTAGCCGA TTTTCAGTTT CGCAGACAGG AGACCTCACC
    ATTACGAACG TCCAGCGGTC TGATGTCGGC TACTACATCT GTCAGACTTT AAATGTTGCT
    GGAAGTATCA TCACAAAGGC CTATTTGGAA GTTACTGATG TGATTGCAGA CCGGCCTCCC
    CCAGTCATTC GGCAAGGTCC TGTGAATCAG ACTGTAGCAG TGGATGGCAC TTTAATCCTC
    AGCTGTGTGG CCACAGGCAG TCCAGCACCC ACAATTCTGT GGAGGAAGGA TGGAGTCCTT
    GTTTCAACCC AAGATTCTCG GATCAAACAG CTGGAGAGTG GCGTGCTGCA GATCCGCTAC
    GCTAAGCTGG GTGACACGGG TCGGTACACC TGCACCGCAT CGACCCCCAG CGGTGAGGCC
    ACGTGGAGCG CTTACATCGA AGTCCAGGAA TTTGGGGTTC CAGTTCAACC CCCAAGACCC
    ACCGACCCCA ACTTAATCCC CAGCGCCCCC TCAAAGCCCG AAGTTACAGA TGTCAGCAAA
    AACACGGTCA CTTTATCGTG GCAGCCAAAC TTGAACTCAG GAGCAACCCC AACATCTTAT
    ATTATAGAAG CCTTCAGCCA TGCATCTGGT AGCAGCTGGC AGACGGCGGC AGAAAATGTG
    AAAACAGAAA CATTTGCCAT CAAAGGACTC AAACCTAACG CCATTTACCT TTTCCTGGTG
    AGGGCAGCAA ATGCCTATGG AATTAGTGAT CCAAGCCAAA TATCAGATCC AGTGAAAACG
    CAAGATGTCC CACCAACCAG CCAAGGGGTG GACCACAAGC AGGTCCAAAG AGAACTGGGG
    AATGTTGTCT TACATCTGCA CAACCCGACC ATCCTTTCTT CCTCCTCAGT CGAAGTGCAC
    TGGACGGTGG ACCAACAATC TCAATATATT CAAGGCTATA AAATTCTCTA CCGCCCATCT
    GGAGCCAGCC ATGGTGAATC GGAGTGGTTA GTTTTTGAAG TGAGGACGCC AACGAAAAAC
    AGCGTGGTGA TCCCCGATCT CAGAAAAGGC GTCAACTACG AGATTAAGGC TCGCCCATTT
    TTTAATGAGT TTCAAGGAGC AGACAGTGAA ATCAAATTTG CCAAAACCTT AGAAGAAGCA
    CCAAGTGCCC CTCCCCGAAG TGTAACTGTA TCCAAGAATG ATGGAAATGG GACTGCAATC
    CTGGTCACTT GGCAACCGCC TCCTGAAGAC ACACAAAATG GAATGGTCCA AGAATATAAG
    GTTTGGTGTC TCGGTAATGA AACGAAGTAT CACATAAACA AGACAGTCGA TGGCTCGACC
    TTCTCGGTGG TCATCCCCTC TCTGGTTCCT GGGATCCGAT ACAGTGTGGA GGTGGCAGCG
    AGCACTGGGG CGGGTCCCGG GGTGAAGAGC GAACCTCAGT TCATCCAGTT AGATTCTCAC
    GGAAACCCCG TGTCTCCCGA GGACCAAGTC AGCCTTGCTC AGCAGATCTC CGACGTGGTG
    AGGCAGCCGG CGTTCATTGC AGGCATTGGG GCAGCCTGTT GGATTATCCT CATGGTCTTC
    AGCATCTGGC TTTACAGACA CCGCAAGAAG AGAAACGGAC TCACCAGCAC GTATGCGGGT
    ATCCGGAAAG TCCCGTCCTT TACCTTCACG CCAACAGTAA CTTATCAAAG AGGAGGCGAA
    GCCGTCAGCA GTGGAGGGAG GCCGGGACTC CTCAACATTA GTGAACCTGC CACGCAGCCA
    TGGCTGGCAG ACACGTGGCC CAACACAGGC AACAACCACA ATGACTGCTC CATCAACTGC
    TGCACGGCCG GCAATGGCAA CAGCGACAGC AACCTGACAA CCTACAGTCG CCCAGCTGAT
    TGTATAGCCA ATTATAACAA CCAACTGGAT AACAAACAAA CAAATCTGAT GCTCCCTGAG
    TCAACTGTTT ATGGTGATGT GGACCTTAGT AACAAAATCA ATGAGATGAA AACCTTCAAT
    AGCCCAAATC TGAAGGACGG GCGTTTTGTC AATCCATCAG GGCAGCCCAC TCCCTATGCC
    ACCACTCAAC TCATCCAGGC GAACCTCAGC AACAATATGA ATAATGGCGC CGGGGACTCC
    AGCGAGAAGC ACTGGAAGCC TCCGGGACAG CAGAAACCGG AAGTGGCACC AATTCAGTAT
    AACATCATGG AGCAAAACAA GCTGAACAAA GATTATCGGG CAAATGACAC GATCCCTCCA
    ACCATCCCAT ACAACCAGTC CTATGACCAG AACACAGGAG GGTCTTATAA CAGCTCAGAT
    CGGGGCAGTA GTACATCCGG GAGTCAAGGC CACAAGAAAG GGGCACGGAC ACCAAAGGCA
    CCCAAGCAGG GTGGCATGAA CTGGGCAGAC TTGCTGCCCC CTCCTCCGGC GCATCCTCCC
    CCACATAGCA ACAGTGAAGA ATACAACATG TCCGTAGATG AAAGCTATGA TCAAGAAATG
    CCCTGTCCAG TGCCACCTGC GCGGATGTAT TTGCAACAGG ATGAATTAGA GGAAGAGGAA
    GATGAAAGAG GCCCCACTCC CCCTGTCCGG GGGGCAGCTT CTTCTCCAGC TGCTGTGTCC
    TACAGCCACC AATCCACGGC CACTCTCACC CCTTCTCCCC AGGAAGAGCT CCAGCCCATG
    TTACAGGATT GTCCGGAGGA CCTCGGGCAC ATGCCCCACC CACCAGACAG GAGGCGGCAG
    CCCGTGAGTC CTCCTCCACC GCCACGGCCA ATTTCCCCAC CTCATACTTA CGGCTACATT
    TCAGGACCCC TTGTCTCAGA TATGGATACC GATGCCCCAG AAGAGGAGGA GGACGAAGCT
    GACATGGAAG TTGCCAAAAT GCAGACCCGA AGGCTGTTGT TACGTGGGCT GGAGCAGACC
    CCAGCATCCA GTGTTGGGGA CCTTGAGAGC TCCGTCACTG GGTCCATGAT CAATGGCTGG
    GGCTCAGCCT CGGAAGAGGA CAACATTTCC AGTGGGCGCT CCAGTGTCAG TTCATCCGAT
    GGCTCCTTTT TCACTGACGC TGATTTTGCC CAGGCAGTTG CTGCAGCTGC GGAGTATGCG
    GGCCTGAAAG TGGCTCGCCG CCAAATGCAA GATGCTGCTG GCCGCCGCCA CTTCCATGCC
    TCTCAGTGCC CAAGGCCCAC GAGTCCTGTG TCCACAGACA GCAACATGAG TGCTGTTGTG
    ATCCAGAAAG CCAGACCCGC CAAGAAGCAG AAACACCAGC CAGGACATCT GCGCAGGGAA
    GCCTACGCAG ATGATCTTCC ACCCCCTCCA GTGCCACCAC CTGCTATAAA ATCGCCCACT
    GTCCAGTCCA AGGCACAGCT GGAGGTACGG CCTGTCATGG TGCCAAAACT CGCGTCTATA
    GAAGCAAGGA CAGATAGATC GTCAGACAGA AAAGGAGGCA GTTACAAGGG GAGAGAAGCT
    CTGGATGGAA GACAAGTCAC TGACCTGCGA ACAAATCCAA GTGACCCCAG AGAGGCACAG
    GAACAGCCAA ATGACGGGAA AGGCCGAGGA ACAAAGGCAG CCAAGCGAGA CCTTCCACCA
    GCAAAGACGC ACCTCGGCCA AGAGGATATT CTTCCGTACT GTAGACCTAC TTTCCCAACG
    TCAAACAACC CTAGAGACCC CAGTTCCTCC AGCTCTATGT CATCAAGAGG ATCAGGGAGC
    CGACAAAGAG AGCAAGCAAA TGTGGGCCGA AGGAATATGG CAGAAATGCA AGTACTTGGG
    GGATTTGAAA GAGGAGATGA GAATAATGAA GAATTAGAGG AAACTGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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