Sptbn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptbn2
Entrez Gene ID	20743
Full Name	spectrin beta, non-erythrocytic 2
Synonyms	Spnb3,mKIAA0302
General protein information	Preferred Names spectrin beta, non-erythrocytic 2 Names spectrin beta chain, non-erythrocytic 2 beta-spectrin 3 spectrin beta 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 4.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006531704.3	XP_006531767.1	spectrin beta chain, non-erythrocytic 2 isoform X2
XM_006531703.3	XP_006531766.1	spectrin beta chain, non-erythrocytic 2 isoform X1
XM_006531705.3	XP_006531768.1	spectrin beta chain, non-erythrocytic 2 isoform X2
XM_006531703.4	XP_006531766.1	spectrin beta chain, non-erythrocytic 2 isoform X1
XM_006531704.4	XP_006531767.1	spectrin beta chain, non-erythrocytic 2 isoform X2
XM_006531705.4	XP_006531768.1	spectrin beta chain, non-erythrocytic 2 isoform X2
NM_021287.1	NP_067262.1	spectrin beta chain, non-erythrocytic 2
NM_021287.2	NP_067262.1	spectrin beta chain, non-erythrocytic 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPTBN2	NP_008877.1
	SPTBN2	XP_001108262.2
Mus musculus (house mouse)	Sptbn2	NP_067262.1
Xenopus tropicalis (tropical clawed frog)	LOC100486199	XP_004913752.1
Pan troglodytes (chimpanzee)	SPTBN2	XP_001172486.2
Danio rerio (zebrafish)	LOC558044	NP_001093459.1
Rattus norvegicus (Norway rat)	Sptbn2	NP_062040.1
Canis lupus familiaris (dog)	SPTBN2	XP_540827.3

Gene Ontology
Bibliography

Related articles in PubMed

?III Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons.
Efimova N, Korobova F, Stankewich MC, Moberly AH, Stolz DB, Wang J, Kashina A, Ma M, Svitkina T
The Journal of neuroscience : the official journal of the Society for Neuroscience37(27)6442-6459(2017 Jul)

Transcription factor Emx2 controls stereociliary bundle orientation of sensory hair cells.
Jiang T, Kindt K, Wu DK
eLife6(2017 Mar)

Posterior cerebellar Purkinje cells in an SCA5/SPARCA1 mouse model are especially vulnerable to the synergistic effect of loss of ?-III spectrin and GLAST.
Perkins EM, Suminaite D, Clarkson YL, Lee SK, Lyndon AR, Rothstein JD, Wyllie DJ, Tanaka K, Jackson M
Human molecular genetics25(20)4448-4461(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TGF-beta/beta2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome.
Title: TGF-?/?2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome.

Study shows a direct interaction between beta-III spectrin and erythroid ankyrin in the cerebellum and to demonstrate a critical role for beta-III spectrin in maintaining ankyrin R throughout the Purkinje cell dendritic tree.
Title: ?-III spectrin underpins ankyrin R function in Purkinje cell dendritic trees: protein complex critical for sodium channel activity is impaired by SCA5-associated mutations.

This study demonistrated that beta-III spectrin is critical for development of purkinje cell dendritic tree and spine morphogenesis.
Title: ?-III spectrin is critical for development of purkinje cell dendritic tree and spine morphogenesis.

Beta-III spectrin mutation L253P interferes with binding to Arp1 and protein trafficking from the Golgi.
Title: Beta-III spectrin mutation L253P associated with spinocerebellar ataxia type 5 interferes with binding to Arp1 and protein trafficking from the Golgi.

betaIII spectrin disruption impairs synaptogenesis by disturbing the intracellular pathways selectively regulating protein trafficking to the synapse
Title: Targeted deletion of betaIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypes.

The following Sptbn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptbn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14702	NM_021287.2 Latest version!	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1301.30 $1859.00
OMu14702	XM_006531704.4 Latest version!	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu14702	XM_006531705.4 Latest version!	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu55041	XM_006531703.4 Latest version!	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OMu55041	XM_006531703.3	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu14702	XM_006531704.3	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1301.30 $1859.00
OMu14702	XM_006531705.3	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1301.30 $1859.00
OMu14702	NM_021287.1	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptbn2 ( NM_021287.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067262.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), mRNA.

Price & Availability↑

CloneID	OMu14702
Clone ID Related Accession (Same CDS sequence)	XM_006531704.3 , XM_006531705.3 , XM_006531704.4 , XM_006531705.4 , NM_021287.1 , NM_021287.2
Accession Version	NM_021287.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-24
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079860.1. On Nov 26, 2004 this sequence version replaced XM_129130.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC079860.1, AK147671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067262.1
CDS	445..7611
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	NM_021287.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021287.1

ORF Insert Sequence:

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( NM_021287.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067262.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), mRNA.

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CloneID	OMu14702
Clone ID Related Accession (Same CDS sequence)	XM_006531704.3 , XM_006531705.3 , XM_006531704.4 , XM_006531705.4 , NM_021287.1 , NM_021287.2
Accession Version	NM_021287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-20
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ119420.1, BC079860.1, AC147618.3 and BE993578.1. On Jul 24, 2018 this sequence version replaced NM_021287.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147671.1, BC079860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067262.1
CDS	460..7626
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	NM_021287.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021287.2

ORF Insert Sequence:

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_006531704.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531767.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.

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CloneID	OMu14702
Clone ID Related Accession (Same CDS sequence)	XM_006531704.3 , XM_006531705.3 , XM_006531704.4 , XM_006531705.4 , NM_021287.1 , NM_021287.2
Accession Version	XM_006531704.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531767.1
CDS	107..7273
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_006531704.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531704.3

ORF Insert Sequence:

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_006531705.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531768.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.

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CloneID	OMu14702
Clone ID Related Accession (Same CDS sequence)	XM_006531704.3 , XM_006531705.3 , XM_006531704.4 , XM_006531705.4 , NM_021287.1 , NM_021287.2
Accession Version	XM_006531705.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531768.1
CDS	103..7269
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_006531705.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531705.3

ORF Insert Sequence:

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_006531704.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531767.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.

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CloneID	OMu14702
Clone ID Related Accession (Same CDS sequence)	XM_006531704.3 , XM_006531705.3 , XM_006531704.4 , XM_006531705.4 , NM_021287.1 , NM_021287.2
Accession Version	XM_006531704.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531704.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531767.1
CDS	657..7823
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_006531704.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531704.4

ORF Insert Sequence:

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_006531705.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531768.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.

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CloneID	OMu14702
Clone ID Related Accession (Same CDS sequence)	XM_006531704.3 , XM_006531705.3 , XM_006531704.4 , XM_006531705.4 , NM_021287.1 , NM_021287.2
Accession Version	XM_006531705.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531705.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531768.1
CDS	418..7584
Translation	MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_006531705.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531705.4

ORF Insert Sequence:

ATGAGCAGCA CTCTGTCACC CACTGACTTT GACAGCTTGG AGATCCAGGG CCAGTACAGT 
GACATCAACA ACCGCTGGGA CCTGCCTGAC TCAGACTGGG ACAATGACAG CAGTTCGGCC 
CGCCTCTTTG AGAGGTCCAG GATCAAGGCC CTGGCAGATG AGCGAGAAGC CGTGCAGAAG 
AAAACCTTCA CCAAGTGGGT GAACTCCCAC CTGGCCCGGG TGACATGCCG GGTGGGAGAC 
CTGTACAGCG ACCTGCGGGA CGGGCGCAAC CTCCTGAGGC TCCTGGAGGT GCTCTCGGGA 
GAGACCCTGC CAAAACCTAC CAAGGGCCGA ATGCGGATTC ACTGCCTGGA GAATGTAGAT 
AAAGCGCTGC AGTTCCTGAA GGAGCAGAAG GTGCACTTGG AAAACATGGG CTCCCATGAC 
ATTGTGGACG GAAACCACCG TCTGACCCTT GGGCTAGTGT GGACCATCAT CCTCCGATTT 
CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT 
GCCCTGCTGC TGTGGTGCCA GATGAAGACT GCAGGATACC CCAATGTCAA TGTGCACAAC 
TTTACCACCA GTTGGAGAGA TGGGCTGGCC TTTAATGCCA TTGTGCACAA ACACCGGCCA 
GACCTGTTGG ATTTTGAGTC CCTGAAGAAG TGTAATGCAC ACTACAATCT GCAGAATGCT 
TTCAATCTGG CTGAAAAGGA ACTTGGCCTG ACGAAGCTCC TGGATCCTGA AGATGTGAAT 
GTAGACCAGC CCGACGAGAA GTCTATCATC ACCTACGTGG CCACTTACTA CCACTACTTC 
TCCAAGATGA AGGCCCTGGC TGTGGAAGGC AAAAGAATTG GCAAGGTCCT AGACCATGCC 
ATGGAGGCAG AACACCTGGT AGAGAAATAC GAGTCCCTGG CCTCTGAACT GCTGCAGTGG 
ATTGAGCAAA CGATCGTGAC CCTCAATGAC CGACAGCTGG CCAACTCCCT GAGTGGAGTC 
CAGAACCAGC TGCAGTCTTT CAACTCCTAT CGCACTGTGG AGAAGCCACC CAAGTTCACG 
GAGAAAGGGA ACTTGGAGGT GTTGCTCTTC ACCATCCAGA GCAAGCTACG GGCCAACAAC 
CAGAAGGTCT ACACACCCCG GGAAGGCCGG CTCATCTCGG ACATCAACAA GGCCTGGGAG 
CGGCTGGAGA AAGCCGAACA TGAGCGAGAG CTAGCGCTGC GCACAGAGCT GATCCGCCAG 
GAGAAGCTGG AGCAACTGGC TGCTCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG 
CTCAGTGAGA ACCAGCGACT TGTGTCCCAG GACAACTTTG GGCTGGAACT GGCAGCAGTG 
GAGGCTGCAG TGAGAAAGCA TGAAGCCATT GAGACAGACA TTGTCGCCTA CAGTGGCCGG 
GTGCAGGCGG TGGATGCTGT AGCTGCAGAA CTGGCTGCTG AGCACTACCA TGACATTAAG 
CGCATTGCGG CACGTCAGAA CAACGTGGCC CGGCTCTGGG ACTTCTTACG GCAAATGGTG 
GCTGCCCGCC GTGAGAGACT TCTTCTCAAC CTGGAGCTGC AGAAAGTGTT TCAGGACCTG 
CTCTACCTCA TGGACTGGAT GGCAGAGATG AAGGGCCGAC TGCAGTCTCA GGACTTGGGC 
AAGCACCTGG CTGGAGTGGA GGACCTCCTG CAGCTACACG AACTGGTGGA GGCGGACATC 
GCGGTTCAGG CTGAGAGGGT TCGAGCGGTC AGCGCCTCTG CCCTGCGTTT CTGCGACCCA 
GGGAAAGAGT ATAGGCCATG TGACCCACAG CTGGTGTCAG AGAGGGTGGC CACTCTGGAG 
CAGAGCTATG AGGCCCTGTG TGAATTGGCA GCGACTAGAA GGGCCCGACT GGAAGAGTCA 
CGTCGTCTCT GGAGGTTCCT CTGGGAAGTG GGTGAGGCTG AGGCCTGGGT CCGGGAGCAG 
CAGCACCTCC TGGCCTCAGC TGATACAGGC CGGGACCTGA CTGGTGTCCT TCGCCTGCTC 
AACAAGCACG CAGCCCTACG GGGTGAGATG AGTGGCCGTC TGGGGCCCCT GAAGCTCACC 
CTGGAGCAAG GTCAGCAGTT AGTCGCTGAG GGCCACCCTG GAGCCAACCA AGCCTCAACC 
CGTGCAGCCG AGCTCCAGGC CCAGTGGGAG CGACTAGAAG CCCTGGCCGA GGAGCGAGCC 
CAGCAGCTGG CACAGGCTGC CAGCCTCTAC CAGTTCCAGG CAGATGCAAA TGACATGGAG 
GCCTGGTTGG TGGATGCACT GCGCCTGGTA TCTAGCCCCG AGGTAGGGCA CGACGAGTTC 
TCCACACAGG CCCTGGCCAG GCAGCACAGG GCCCTTGAAG AGGAGATCCG AGCCCACCGG 
CCTACACTGG ATGCCCTGAG GGAGCAGGCT GCAGCCCTGC CACCTGCACT GAGCCACACA 
CCGGAGGTAC AGGGCAGGGT GCCCACTCTG GAGCAGCACT ATGAGGAGCT GCAGGCCCGA 
GCCGGTGAGC GTGCACGAGC CCTGGAGGCA GCCCTGGCGT TCTATACCAT GCTCAGCGAG 
GCTGGGGCCT GTGGCCTCTG GGTAGAGGAG AAGGAGCAAT GGCTCAACGG GCTGGCCCTA 
CCTGAGCGCC TGGAGGACCT GGAGGTGGTC CAGCAGAGGT TTGAGACCCT GGAGCCTGAA 
ATGAACGCCC TGGCTGCACG GGTTACTGCT GTCAATGACA TCGCTGAGCA GCTGCTGAAG 
GCCAGTCCAC CAGGCAAGGA CCGCATCATT GGCACCCAGG AGCAGCTCAA CCAAAGGTGG 
CAGCAGTTTC GGTCCCTGGC AGATGGCAAA AAGGCGGCTC TGACATCAGC CCTGAGCATC 
CAGAATTACC ACCTAGAGTG CACAGAGACC CAGGCCTGGA TGAGAGAGAA GACTAAGGTC 
ATTGAGTCCA CCCAGGGCCT GGGCAATGAT CTAGCTGGTG TGCTGGCCCT GCAGCGGAAG 
TTGGCGGGTA CTGAGAGAGA TCTGGAAGCT ATCTCTGCCC GGGTGGGTGA GCTGACCCAA 
GAGGCAAATG CCTTGGCTGC TGGGCACCCA GCTCAAGCTC CTGCCATCAA CACCCGGCTT 
GGAGAGGTAC AAGCAGGATG GGAAGATCTT CGGGCGACCA TGAGGCGGAG AGAAGAGTCC 
CTGGGTGAGG CTCGGCGGCT GCAGGATTTC CTGCGCAGCT TGGATGACTT CCAGGCCTGG 
CTAGGCCGCA CACAGACCGC TGTAGCCTCC GAAGAAGGGC CAGCCACCCT TCCCGAGGCA 
GAAGCCCTCT TAGCCCAGCA CGCAGCTCTG CGGGGAGAAG TGGAGAGAGC CCAGAGCGAG 
TACAGCCGCC TCAGGACCTT GGGCGAGGAG GTGACCAGAG ACCAGGCTGA TCCCCAGTGC 
CTCTTCCTCA GACAGAGGCT GGAAGCCCTT GGAACCGGCT GGGAGGAGCT GGGTCGCATG 
TGGGAGAGCC GGCAAGGCCG CCTGGCCCAG GCCCATGGCT TCCAGGGGTT TTTAAGAGAT 
GCTCGCCAGG CTGAGGGTGT TCTCAGCAGC CAGGAATATG TCTTGTCTCA CACGGAGATG 
CCAGGGACAC TGCAGGCGGC AGATGCAGCC ATTAAAAAGC TGGAAGACTT CATGAGCACT 
ATGGACGCCA ATGGAGAGCG CATCCGCGGA CTCCTGGAGG CTGGACGTCA GCTGGTGTCC 
AAGGGCAATA TCCATGCTGA GAAGATCCAA GAGAAGGCGG ACTCCATCGA GAAGAGGCAC 
AGAAAGAATC AGGAGGCGGT GCAGCAGCTT TTAGGACGCC TTCGGGACAA CCGAGAGCAG 
CAGCACTTCT TGCAAGACTG TCAGGAGCTG AGACTCTGGA TCGATGAGAA GATGCTGACA 
GCTCAGGATG TGTCCTATGA CGAAGCTCGC AACCTGCACA CCAAGTGGCA GAAACACCAG 
GCATTCATGG CCGAGCTTGC AGCCAACAAG GACTGGCTGG ACAAAGTGGA CAAGGAAGGG 
CGGGAGCTGA CTCTTGAAAA GCCAGAACTC AAAGTCGTAG TGTCAGAGAA GCTGGAGGAC 
CTGCACCGGC GCTGGGATGA ACTGGAGACC ACTACCCAAG CCAAGGCCCG CAGTCTTTTT 
GATGCCAACC GGGCAGAGCT ATTTGCTCAA AGCTGTTCTG CCCTGGAGAG CTGGCTGGAG 
AGCCTGCAGG CCCAGCTGCA CTCAGATGAC TATGGCAAAG ACCTCACCAG TGTCAACATT 
CTGCTGAAGA AGCAACAGAT GCTGGAACGA GAGATGGCTG TGAGAGAGAA GGAGGTAGAG 
GCGATACAGG CCCAGGCCCA GGCCCTGGCC CAGGAAGACC AGAGTGCAGG GGAGGTAGAA 
AGGACCTCAA GGGCTGTGGA GGAGAAGTTC AGGGCCTTGT GTCAACCCAT GAAAGAACGC 
TGCCGGCGCC TGCACGCCTC CCGGGAGCAG CACCAGTTCC ACCGGGATGT GGAGGATGAG 
ATATTGTGGG TGACTGAGCG GCTTCCCATG GCCAGCTCTC TGGAACATGG CAAGGACTTG 
CCCAGTGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAGGAGAT CCAGGGCCAT 
GAGCCCCGGA TTGCAGACCT CAAAGAGAGG CAGCGCACTC TGGGAACAGC AGCAGCAGGT 
CCAGAGCTGG CTGAGCTCCA AGAAATGTGG AAACGCCTGA GCCATGAGCT GGAGCTTCGG 
GGCAAGCGGC TGGAGGAAGC TCTTCGAGCC CAGCAATTCT ATCGTGATGC TGCAGAGGCC 
GAGGCTTGGA TGGGTGAGCA GGAGTTACAC ATGATGGGCC AGGAAAAGGC CAAGGATGAG 
CTGAGCGCCC AGGCAGAAGT GAAGAAGCAT CAGGTATTGG AACAAGCCCT TGCTGACTAT 
GCCCAGACCA TCAAACAACT AGCAGCCAGC AGTCAAGATA TGATTGACCA TGAACATCCA 
GAGAGCACAA GACTAACAAT ACGCCAAGCC CAGGTGGACA AGCTGTACGC CAGCCTGAAG 
GAGCTGGCAG GAGAGCGGCG CGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC 
AGAGAGCTGG ATGACCTGGA ACAGTGGATA CAGGAGCGTG AAGTTGTGGC AGCCTCCCAT 
GAACTGGGCC AAGACTATGA GCACGTGACT ATGCTTCGGG ACAAATTCCG AGAGTTCTCC 
AAGGACACCA GCACCATTGG CCAGGAGCGC GTAGACAGTG CCAATGCACT GGCCAATGGG 
CTCATTGCTG GGGGCCATGC TGCACGGGCC ACTGTGGCCG AGTGGAAGGA CAGTCTCAAT 
GAGGCCTGGG CTGACCTGCT GGAGCTGCTG GACACAAGAG GTCAGGTGCT GGCTGCTGCG 
TATGAGCTGC AGCGCTTCCT GCATGGAGCC CGCCAAGCCC TGGCACGGGT ACAGCACAAG 
CAGCAGCAGC TTCCAGACGG GACTGGCCGT GACCTCAATG CTGCTGAGGC CCTGCAGCGC 
CGGCACTGTG CCTATGAGCA CGACATCCAA GCCCTCAGCA CTCAGGTCCA GCAAGTTCAA 
GATGATGGCC AAAGGCTACA GAAGGCCTAT GCTGGAGACA AGGCTGAGGA AATTGGCCGT 
CACATGCAGG CAGTGGCTGA GGCCTGGGCC CAGCTCCAGG GAAGTTCTGC TGCCCGCCGA 
CAGCTGTTAC TGGATACCAC GGACAAATTC CGATTCTTTA AGGCTGTCCG AGAGTTGATG 
CTGTGGATGG ATGGGATTAA CCTGCAGATG GATGCCCAGG AGCGGCCCCG GGATGTGTCC 
TCTGCAGATT TAGTCATCAA AAACCAACAA GGAATCAAAG CAGAGATAGA GGCCAGAGCA 
GACAGGTTCT CCTCCTGCAT TGACATGGGG CAGGAGCTGC TGGCCCGGAG CCACTATGCT 
GCTGAGGAGA TCTCAGAGAA GCTGTCTCAG CTACAGTCCC GGCGCCAGGA GACAGCTGAC 
AAGTGGCAGG AGAAGATGGA CTGGCTACAG CTTGTTTTGG AGGTGCTTGT GTTTGGGAGA 
GATGCAGGGA TGGCGGAGGC CTGGCTGTGC AGTCAGGAGC CATTGGTGCG AAGTGCAGAA 
CTGGGTTGCA CTGTGGACGA AGTAGAGAGC CTCATCAAGC GACACGAAGC CTTTCAGAAG 
TCGGCCGTGG CCTGGGAAGA GCGTTTCAGT GCGCTGGAGA AGCTCACTGC GCTGGAAGAG 
CGGGAAAATG AGCGGAAAAG GAAGAGGGAG GAGGAGGAAC GGAGGAAACA GCCTCCTACT 
TCAGAGCCCA TGGCTAGTCA GCCAGAAGGG AGTCTGGTAG ATGGCCAGAG AGTTCCTGAC 
ACTGCCTGGG ATGGGACTCA GTCAAAATTG CCACCATCCA CACAAGCACC CAGTGTTAAT 
GGGGTCTGCA CGGACACCGA CTCCTCACAG CCCCTGTTGG AACAGCAGAG ACTTGAACAG 
AGCAATGTCC CCGAAGGACC TGGATCTGGC ACAGGAGACG AGTCCAGTGG GCCCCGGGGA 
GAGAGGCAGA CCCTGCCCCG GGGCCCTGCT CCTTCTCCAA TGCCCCAGAG CAGATCATCT 
GAAGCAGCTC ATGGTGCCAC CCTGCCCACA CGAGGACCTG AGCTCTCTGC TCAGGAACAG 
ATGGAAGGGA TGCTGTGCCG CAAACAAGAG ATGGAAGCCT TCAACAAGAA AGCTGCCAAC 
AGGTCATGGC AGAATGTGTA CTGTGTACTT CGGCGTGGGA GCCTCGGCTT TTACAAGGAT 
GCCAGGGCAG CTAGTGCAGG AGTGCCCTAC CATGGAGAAG TGCCTGTCAG CCTGGCCAGG 
GCCCAGGGAA GTGTGGCCTT TGATTATCGG AAACGCAAAC ATGTTTTCAA GCTGGGCTTA 
CAGGATGGCA AAGAATACCT ATTCCAGGCC AAGGACGAGG CAGAGATGAG CTCATGGCTG 
AGAGTGGTGA ATGCAGCCAT TGCCACTGCA TCCTCTGCCC CTGGAGAGTC AGAAGAGCCA 
GTGGTGCCCA GTGCCAGCCG GGGTCTGACC CGGGCCATGA CCATGCCCCC AGTGTCACAG 
CCTGAGGGTT CCATCGTGCT TCGAAGCAAG GATGGCAGAG AAAGAGAGCG AGAAAAACGA 
TTCAGCTTCT TTAAGAAGAA CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_006531703.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531766.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.

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CloneID	OMu55041
Clone ID Related Accession (Same CDS sequence)	XM_006531703.3 , XM_006531703.4
Accession Version	XM_006531703.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531703.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACAA ACGGAGTGGG CAGGGTCCAT GGGAACGGCC TGAACGGGGC TGCTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAGAAC CCTGGGGGCG TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GCTTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCTA CCAAGGGCCG AATGCGGATT 
CACTGCCTGG AGAATGTAGA TAAAGCGCTG CAGTTCCTGA AGGAGCAGAA GGTGCACTTG 
GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGATAC 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAATGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA TGTAGACCAG CCCGACGAGA AGTCTATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCCAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGAATT 
GGCAAGGTCC TAGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA CGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATTGAGCAA ACGATCGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGAGT CCAGAACCAG CTGCAGTCTT TCAACTCCTA TCGCACTGTG 
GAGAAGCCAC CCAAGTTCAC GGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGCAAGCTAC GGGCCAACAA CCAGAAGGTC TACACACCCC GGGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTGGAG AAAGCCGAAC ATGAGCGAGA GCTAGCGCTG 
CGCACAGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGAC TTGTGTCCCA GGACAACTTT 
GGGCTGGAAC TGGCAGCAGT GGAGGCTGCA GTGAGAAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTCGCCT ACAGTGGCCG GGTGCAGGCG GTGGATGCTG TAGCTGCAGA ACTGGCTGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCACGTCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GGCAAATGGT GGCTGCCCGC CGTGAGAGAC TTCTTCTCAA CCTGGAGCTG 
CAGAAAGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGA 
CTGCAGTCTC AGGACTTGGG CAAGCACCTG GCTGGAGTGG AGGACCTCCT GCAGCTACAC 
GAACTGGTGG AGGCGGACAT CGCGGTTCAG GCTGAGAGGG TTCGAGCGGT CAGCGCCTCT 
GCCCTGCGTT TCTGCGACCC AGGGAAAGAG TATAGGCCAT GTGACCCACA GCTGGTGTCA 
GAGAGGGTGG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GTGAATTGGC AGCGACTAGA 
AGGGCCCGAC TGGAAGAGTC ACGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCT 
GAGGCCTGGG TCCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGATACAGG CCGGGACCTG 
ACTGGTGTCC TTCGCCTGCT CAACAAGCAC GCAGCCCTAC GGGGTGAGAT GAGTGGCCGT 
CTGGGGCCCC TGAAGCTCAC CCTGGAGCAA GGTCAGCAGT TAGTCGCTGA GGGCCACCCT 
GGAGCCAACC AAGCCTCAAC CCGTGCAGCC GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCAGCTG GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCAA ATGACATGGA GGCCTGGTTG GTGGATGCAC TGCGCCTGGT ATCTAGCCCC 
GAGGTAGGGC ACGACGAGTT CTCCACACAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAA 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCCTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCGGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG AGCCGGTGAG CGTGCACGAG CCCTGGAGGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCTGGGGCC TGTGGCCTCT GGGTAGAGGA GAAGGAGCAA 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAGCAGAGG 
TTTGAGACCC TGGAGCCTGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAATGAC 
ATCGCTGAGC AGCTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTT CGGTCCCTGG CAGATGGCAA AAAGGCGGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAGA AGACTAAGGT CATTGAGTCC ACCCAGGGCC TGGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GTTGGCGGGT ACTGAGAGAG ATCTGGAAGC TATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCCTTGGCTG CTGGGCACCC AGCTCAAGCT 
CCTGCCATCA ACACCCGGCT TGGAGAGGTA CAAGCAGGAT GGGAAGATCT TCGGGCGACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGGCGGC TGCAGGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACCG CTGTAGCCTC CGAAGAAGGG 
CCAGCCACCC TTCCCGAGGC AGAAGCCCTC TTAGCCCAGC ACGCAGCTCT GCGGGGAGAA 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAGTG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCCTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTAAGAGA TGCTCGCCAG GCTGAGGGTG TTCTCAGCAG CCAGGAATAT 
GTCTTGTCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CAGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC TATGGACGCC AATGGAGAGC GCATCCGCGG ACTCCTGGAG 
GCTGGACGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCG 
GACTCCATCG AGAAGAGGCA CAGAAAGAAT CAGGAGGCGG TGCAGCAGCT TTTAGGACGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAGACTCTGG 
ATCGATGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ACGAAGCTCG CAACCTGCAC 
ACCAAGTGGC AGAAACACCA GGCATTCATG GCCGAGCTTG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCGTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACCGG CGCTGGGATG AACTGGAGAC CACTACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCCAAC CGGGCAGAGC TATTTGCTCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAA 
GACCTCACCA GTGTCAACAT TCTGCTGAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCGATACAG GCCCAGGCCC AGGCCCTGGC CCAGGAAGAC 
CAGAGTGCAG GGGAGGTAGA AAGGACCTCA AGGGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAACCCA TGAAAGAACG CTGCCGGCGC CTGCACGCCT CCCGGGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATATTGTGG GTGACTGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAACATG GCAAGGACTT GCCCAGTGTC CAGCTTCTCA TGAAGAAAAA CCAGACCCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG GCAGCGCACT 
CTGGGAACAG CAGCAGCAGG TCCAGAGCTG GCTGAGCTCC AAGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGCAAGCGG CTGGAGGAAG CTCTTCGAGC CCAGCAATTC 
TATCGTGATG CTGCAGAGGC CGAGGCTTGG ATGGGTGAGC AGGAGTTACA CATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTATTG 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGACTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTACG CCAGCCTGAA GGAGCTGGCA GGAGAGCGGC GCGAGCGCCT GCAGGAGCAC 
CTCCGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AACAGTGGAT ACAGGAGCGT 
GAAGTTGTGG CAGCCTCCCA TGAACTGGGC CAAGACTATG AGCACGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAAGGACACC AGCACCATTG GCCAGGAGCG CGTAGACAGT 
GCCAATGCAC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACTGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC GTATGAGCTG CAGCGCTTCC TGCATGGAGC CCGCCAAGCC 
CTGGCACGGG TACAGCACAA GCAGCAGCAG CTTCCAGACG GGACTGGCCG TGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGT GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAAGTTCA AGATGATGGC CAAAGGCTAC AGAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCCTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGCCG ACAGCTGTTA CTGGATACCA CGGACAAATT CCGATTCTTT 
AAGGCTGTCC GAGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGCGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGAATCAAA 
GCAGAGATAG AGGCCAGAGC AGACAGGTTC TCCTCCTGCA TTGACATGGG GCAGGAGCTG 
CTGGCCCGGA GCCACTATGC TGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA CAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGG ATGGCGGAGG CCTGGCTGTG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGACG AAGTAGAGAG CCTCATCAAG 
CGACACGAAG CCTTTCAGAA GTCGGCCGTG GCCTGGGAAG AGCGTTTCAG TGCGCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAAAAT GAGCGGAAAA GGAAGAGGGA GGAGGAGGAA 
CGGAGGAAAC AGCCTCCTAC TTCAGAGCCC ATGGCTAGTC AGCCAGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCCTGA CACTGCCTGG GATGGGACTC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGTGTTAA TGGGGTCTGC ACGGACACCG ACTCCTCACA GCCCCTGTTG 
GAACAGCAGA GACTTGAACA GAGCAATGTC CCCGAAGGAC CTGGATCTGG CACAGGAGAC 
GAGTCCAGTG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCTTCTCCA 
ATGCCCCAGA GCAGATCATC TGAAGCAGCT CATGGTGCCA CCCTGCCCAC ACGAGGACCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ATGCTGTGCC GCAAACAAGA GATGGAAGCC 
TTCAACAAGA AAGCTGCCAA CAGGTCATGG CAGAATGTGT ACTGTGTACT TCGGCGTGGG 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCCTA CCATGGAGAA 
GTGCCTGTCA GCCTGGCCAG GGCCCAGGGA AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTTTTCA AGCTGGGCTT ACAGGATGGC AAAGAATACC TATTCCAGGC CAAGGACGAG 
GCAGAGATGA GCTCATGGCT GAGAGTGGTG AATGCAGCCA TTGCCACTGC ATCCTCTGCC 
CCTGGAGAGT CAGAAGAGCC AGTGGTGCCC AGTGCCAGCC GGGGTCTGAC CCGGGCCATG 
ACCATGCCCC CAGTGTCACA GCCTGAGGGT TCCATCGTGC TTCGAAGCAA GGATGGCAGA 
GAAAGAGAGC GAGAAAAACG ATTCAGCTTC TTTAAGAAGA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531766.1
CDS	21..7208
Translation	MNTNGVGRVHGNGLNGAAEFYYEAVEGSQNPGGVLLSPAAFINPAQYASVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_006531703.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531703.4

ORF Insert Sequence:

ATGAATACAA ACGGAGTGGG CAGGGTCCAT GGGAACGGCC TGAACGGGGC TGCTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAGAAC CCTGGGGGCG TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GCTTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCTA CCAAGGGCCG AATGCGGATT 
CACTGCCTGG AGAATGTAGA TAAAGCGCTG CAGTTCCTGA AGGAGCAGAA GGTGCACTTG 
GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGATAC 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAATGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA TGTAGACCAG CCCGACGAGA AGTCTATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCCAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGAATT 
GGCAAGGTCC TAGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA CGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATTGAGCAA ACGATCGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGAGT CCAGAACCAG CTGCAGTCTT TCAACTCCTA TCGCACTGTG 
GAGAAGCCAC CCAAGTTCAC GGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGCAAGCTAC GGGCCAACAA CCAGAAGGTC TACACACCCC GGGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTGGAG AAAGCCGAAC ATGAGCGAGA GCTAGCGCTG 
CGCACAGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGAC TTGTGTCCCA GGACAACTTT 
GGGCTGGAAC TGGCAGCAGT GGAGGCTGCA GTGAGAAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTCGCCT ACAGTGGCCG GGTGCAGGCG GTGGATGCTG TAGCTGCAGA ACTGGCTGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCACGTCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GGCAAATGGT GGCTGCCCGC CGTGAGAGAC TTCTTCTCAA CCTGGAGCTG 
CAGAAAGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGA 
CTGCAGTCTC AGGACTTGGG CAAGCACCTG GCTGGAGTGG AGGACCTCCT GCAGCTACAC 
GAACTGGTGG AGGCGGACAT CGCGGTTCAG GCTGAGAGGG TTCGAGCGGT CAGCGCCTCT 
GCCCTGCGTT TCTGCGACCC AGGGAAAGAG TATAGGCCAT GTGACCCACA GCTGGTGTCA 
GAGAGGGTGG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GTGAATTGGC AGCGACTAGA 
AGGGCCCGAC TGGAAGAGTC ACGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCT 
GAGGCCTGGG TCCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGATACAGG CCGGGACCTG 
ACTGGTGTCC TTCGCCTGCT CAACAAGCAC GCAGCCCTAC GGGGTGAGAT GAGTGGCCGT 
CTGGGGCCCC TGAAGCTCAC CCTGGAGCAA GGTCAGCAGT TAGTCGCTGA GGGCCACCCT 
GGAGCCAACC AAGCCTCAAC CCGTGCAGCC GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCAGCTG GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCAA ATGACATGGA GGCCTGGTTG GTGGATGCAC TGCGCCTGGT ATCTAGCCCC 
GAGGTAGGGC ACGACGAGTT CTCCACACAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAA 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCCTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCGGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG AGCCGGTGAG CGTGCACGAG CCCTGGAGGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCTGGGGCC TGTGGCCTCT GGGTAGAGGA GAAGGAGCAA 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAGCAGAGG 
TTTGAGACCC TGGAGCCTGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAATGAC 
ATCGCTGAGC AGCTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTT CGGTCCCTGG CAGATGGCAA AAAGGCGGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAGA AGACTAAGGT CATTGAGTCC ACCCAGGGCC TGGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GTTGGCGGGT ACTGAGAGAG ATCTGGAAGC TATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCCTTGGCTG CTGGGCACCC AGCTCAAGCT 
CCTGCCATCA ACACCCGGCT TGGAGAGGTA CAAGCAGGAT GGGAAGATCT TCGGGCGACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGGCGGC TGCAGGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACCG CTGTAGCCTC CGAAGAAGGG 
CCAGCCACCC TTCCCGAGGC AGAAGCCCTC TTAGCCCAGC ACGCAGCTCT GCGGGGAGAA 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAGTG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCCTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTAAGAGA TGCTCGCCAG GCTGAGGGTG TTCTCAGCAG CCAGGAATAT 
GTCTTGTCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CAGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC TATGGACGCC AATGGAGAGC GCATCCGCGG ACTCCTGGAG 
GCTGGACGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCG 
GACTCCATCG AGAAGAGGCA CAGAAAGAAT CAGGAGGCGG TGCAGCAGCT TTTAGGACGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAGACTCTGG 
ATCGATGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ACGAAGCTCG CAACCTGCAC 
ACCAAGTGGC AGAAACACCA GGCATTCATG GCCGAGCTTG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCGTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACCGG CGCTGGGATG AACTGGAGAC CACTACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCCAAC CGGGCAGAGC TATTTGCTCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAA 
GACCTCACCA GTGTCAACAT TCTGCTGAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCGATACAG GCCCAGGCCC AGGCCCTGGC CCAGGAAGAC 
CAGAGTGCAG GGGAGGTAGA AAGGACCTCA AGGGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAACCCA TGAAAGAACG CTGCCGGCGC CTGCACGCCT CCCGGGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATATTGTGG GTGACTGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAACATG GCAAGGACTT GCCCAGTGTC CAGCTTCTCA TGAAGAAAAA CCAGACCCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG GCAGCGCACT 
CTGGGAACAG CAGCAGCAGG TCCAGAGCTG GCTGAGCTCC AAGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGCAAGCGG CTGGAGGAAG CTCTTCGAGC CCAGCAATTC 
TATCGTGATG CTGCAGAGGC CGAGGCTTGG ATGGGTGAGC AGGAGTTACA CATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTATTG 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGACTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTACG CCAGCCTGAA GGAGCTGGCA GGAGAGCGGC GCGAGCGCCT GCAGGAGCAC 
CTCCGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AACAGTGGAT ACAGGAGCGT 
GAAGTTGTGG CAGCCTCCCA TGAACTGGGC CAAGACTATG AGCACGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAAGGACACC AGCACCATTG GCCAGGAGCG CGTAGACAGT 
GCCAATGCAC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACTGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC GTATGAGCTG CAGCGCTTCC TGCATGGAGC CCGCCAAGCC 
CTGGCACGGG TACAGCACAA GCAGCAGCAG CTTCCAGACG GGACTGGCCG TGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGT GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAAGTTCA AGATGATGGC CAAAGGCTAC AGAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCCTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGCCG ACAGCTGTTA CTGGATACCA CGGACAAATT CCGATTCTTT 
AAGGCTGTCC GAGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGCGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGAATCAAA 
GCAGAGATAG AGGCCAGAGC AGACAGGTTC TCCTCCTGCA TTGACATGGG GCAGGAGCTG 
CTGGCCCGGA GCCACTATGC TGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA CAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGG ATGGCGGAGG CCTGGCTGTG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGACG AAGTAGAGAG CCTCATCAAG 
CGACACGAAG CCTTTCAGAA GTCGGCCGTG GCCTGGGAAG AGCGTTTCAG TGCGCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAAAAT GAGCGGAAAA GGAAGAGGGA GGAGGAGGAA 
CGGAGGAAAC AGCCTCCTAC TTCAGAGCCC ATGGCTAGTC AGCCAGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCCTGA CACTGCCTGG GATGGGACTC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGTGTTAA TGGGGTCTGC ACGGACACCG ACTCCTCACA GCCCCTGTTG 
GAACAGCAGA GACTTGAACA GAGCAATGTC CCCGAAGGAC CTGGATCTGG CACAGGAGAC 
GAGTCCAGTG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCTTCTCCA 
ATGCCCCAGA GCAGATCATC TGAAGCAGCT CATGGTGCCA CCCTGCCCAC ACGAGGACCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ATGCTGTGCC GCAAACAAGA GATGGAAGCC 
TTCAACAAGA AAGCTGCCAA CAGGTCATGG CAGAATGTGT ACTGTGTACT TCGGCGTGGG 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCCTA CCATGGAGAA 
GTGCCTGTCA GCCTGGCCAG GGCCCAGGGA AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTTTTCA AGCTGGGCTT ACAGGATGGC AAAGAATACC TATTCCAGGC CAAGGACGAG 
GCAGAGATGA GCTCATGGCT GAGAGTGGTG AATGCAGCCA TTGCCACTGC ATCCTCTGCC 
CCTGGAGAGT CAGAAGAGCC AGTGGTGCCC AGTGCCAGCC GGGGTCTGAC CCGGGCCATG 
ACCATGCCCC CAGTGTCACA GCCTGAGGGT TCCATCGTGC TTCGAAGCAA GGATGGCAGA 
GAAAGAGAGC GAGAAAAACG ATTCAGCTTC TTTAAGAAGA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn2 ( XM_006531703.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531766.1 Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.

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CloneID	OMu55041
Clone ID Related Accession (Same CDS sequence)	XM_006531703.3 , XM_006531703.4
Accession Version	XM_006531703.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spectrin beta chain, non-erythrocytic 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACAA ACGGAGTGGG CAGGGTCCAT GGGAACGGCC TGAACGGGGC TGCTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAGAAC CCTGGGGGCG TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GCTTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCTA CCAAGGGCCG AATGCGGATT 
CACTGCCTGG AGAATGTAGA TAAAGCGCTG CAGTTCCTGA AGGAGCAGAA GGTGCACTTG 
GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGATAC 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAATGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA TGTAGACCAG CCCGACGAGA AGTCTATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCCAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGAATT 
GGCAAGGTCC TAGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA CGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATTGAGCAA ACGATCGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGAGT CCAGAACCAG CTGCAGTCTT TCAACTCCTA TCGCACTGTG 
GAGAAGCCAC CCAAGTTCAC GGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGCAAGCTAC GGGCCAACAA CCAGAAGGTC TACACACCCC GGGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTGGAG AAAGCCGAAC ATGAGCGAGA GCTAGCGCTG 
CGCACAGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGAC TTGTGTCCCA GGACAACTTT 
GGGCTGGAAC TGGCAGCAGT GGAGGCTGCA GTGAGAAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTCGCCT ACAGTGGCCG GGTGCAGGCG GTGGATGCTG TAGCTGCAGA ACTGGCTGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCACGTCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GGCAAATGGT GGCTGCCCGC CGTGAGAGAC TTCTTCTCAA CCTGGAGCTG 
CAGAAAGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGA 
CTGCAGTCTC AGGACTTGGG CAAGCACCTG GCTGGAGTGG AGGACCTCCT GCAGCTACAC 
GAACTGGTGG AGGCGGACAT CGCGGTTCAG GCTGAGAGGG TTCGAGCGGT CAGCGCCTCT 
GCCCTGCGTT TCTGCGACCC AGGGAAAGAG TATAGGCCAT GTGACCCACA GCTGGTGTCA 
GAGAGGGTGG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GTGAATTGGC AGCGACTAGA 
AGGGCCCGAC TGGAAGAGTC ACGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCT 
GAGGCCTGGG TCCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGATACAGG CCGGGACCTG 
ACTGGTGTCC TTCGCCTGCT CAACAAGCAC GCAGCCCTAC GGGGTGAGAT GAGTGGCCGT 
CTGGGGCCCC TGAAGCTCAC CCTGGAGCAA GGTCAGCAGT TAGTCGCTGA GGGCCACCCT 
GGAGCCAACC AAGCCTCAAC CCGTGCAGCC GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCAGCTG GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCAA ATGACATGGA GGCCTGGTTG GTGGATGCAC TGCGCCTGGT ATCTAGCCCC 
GAGGTAGGGC ACGACGAGTT CTCCACACAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAA 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCCTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCGGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG AGCCGGTGAG CGTGCACGAG CCCTGGAGGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCTGGGGCC TGTGGCCTCT GGGTAGAGGA GAAGGAGCAA 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAGCAGAGG 
TTTGAGACCC TGGAGCCTGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAATGAC 
ATCGCTGAGC AGCTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTT CGGTCCCTGG CAGATGGCAA AAAGGCGGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAGA AGACTAAGGT CATTGAGTCC ACCCAGGGCC TGGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GTTGGCGGGT ACTGAGAGAG ATCTGGAAGC TATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCCTTGGCTG CTGGGCACCC AGCTCAAGCT 
CCTGCCATCA ACACCCGGCT TGGAGAGGTA CAAGCAGGAT GGGAAGATCT TCGGGCGACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGGCGGC TGCAGGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACCG CTGTAGCCTC CGAAGAAGGG 
CCAGCCACCC TTCCCGAGGC AGAAGCCCTC TTAGCCCAGC ACGCAGCTCT GCGGGGAGAA 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAGTG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCCTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTAAGAGA TGCTCGCCAG GCTGAGGGTG TTCTCAGCAG CCAGGAATAT 
GTCTTGTCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CAGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC TATGGACGCC AATGGAGAGC GCATCCGCGG ACTCCTGGAG 
GCTGGACGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCG 
GACTCCATCG AGAAGAGGCA CAGAAAGAAT CAGGAGGCGG TGCAGCAGCT TTTAGGACGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAGACTCTGG 
ATCGATGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ACGAAGCTCG CAACCTGCAC 
ACCAAGTGGC AGAAACACCA GGCATTCATG GCCGAGCTTG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCGTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACCGG CGCTGGGATG AACTGGAGAC CACTACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCCAAC CGGGCAGAGC TATTTGCTCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAA 
GACCTCACCA GTGTCAACAT TCTGCTGAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCGATACAG GCCCAGGCCC AGGCCCTGGC CCAGGAAGAC 
CAGAGTGCAG GGGAGGTAGA AAGGACCTCA AGGGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAACCCA TGAAAGAACG CTGCCGGCGC CTGCACGCCT CCCGGGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATATTGTGG GTGACTGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAACATG GCAAGGACTT GCCCAGTGTC CAGCTTCTCA TGAAGAAAAA CCAGACCCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG GCAGCGCACT 
CTGGGAACAG CAGCAGCAGG TCCAGAGCTG GCTGAGCTCC AAGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGCAAGCGG CTGGAGGAAG CTCTTCGAGC CCAGCAATTC 
TATCGTGATG CTGCAGAGGC CGAGGCTTGG ATGGGTGAGC AGGAGTTACA CATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTATTG 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGACTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTACG CCAGCCTGAA GGAGCTGGCA GGAGAGCGGC GCGAGCGCCT GCAGGAGCAC 
CTCCGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AACAGTGGAT ACAGGAGCGT 
GAAGTTGTGG CAGCCTCCCA TGAACTGGGC CAAGACTATG AGCACGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAAGGACACC AGCACCATTG GCCAGGAGCG CGTAGACAGT 
GCCAATGCAC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACTGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC GTATGAGCTG CAGCGCTTCC TGCATGGAGC CCGCCAAGCC 
CTGGCACGGG TACAGCACAA GCAGCAGCAG CTTCCAGACG GGACTGGCCG TGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGT GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAAGTTCA AGATGATGGC CAAAGGCTAC AGAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCCTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGCCG ACAGCTGTTA CTGGATACCA CGGACAAATT CCGATTCTTT 
AAGGCTGTCC GAGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGCGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGAATCAAA 
GCAGAGATAG AGGCCAGAGC AGACAGGTTC TCCTCCTGCA TTGACATGGG GCAGGAGCTG 
CTGGCCCGGA GCCACTATGC TGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA CAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGG ATGGCGGAGG CCTGGCTGTG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGACG AAGTAGAGAG CCTCATCAAG 
CGACACGAAG CCTTTCAGAA GTCGGCCGTG GCCTGGGAAG AGCGTTTCAG TGCGCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAAAAT GAGCGGAAAA GGAAGAGGGA GGAGGAGGAA 
CGGAGGAAAC AGCCTCCTAC TTCAGAGCCC ATGGCTAGTC AGCCAGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCCTGA CACTGCCTGG GATGGGACTC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGTGTTAA TGGGGTCTGC ACGGACACCG ACTCCTCACA GCCCCTGTTG 
GAACAGCAGA GACTTGAACA GAGCAATGTC CCCGAAGGAC CTGGATCTGG CACAGGAGAC 
GAGTCCAGTG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCTTCTCCA 
ATGCCCCAGA GCAGATCATC TGAAGCAGCT CATGGTGCCA CCCTGCCCAC ACGAGGACCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ATGCTGTGCC GCAAACAAGA GATGGAAGCC 
TTCAACAAGA AAGCTGCCAA CAGGTCATGG CAGAATGTGT ACTGTGTACT TCGGCGTGGG 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCCTA CCATGGAGAA 
GTGCCTGTCA GCCTGGCCAG GGCCCAGGGA AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTTTTCA AGCTGGGCTT ACAGGATGGC AAAGAATACC TATTCCAGGC CAAGGACGAG 
GCAGAGATGA GCTCATGGCT GAGAGTGGTG AATGCAGCCA TTGCCACTGC ATCCTCTGCC 
CCTGGAGAGT CAGAAGAGCC AGTGGTGCCC AGTGCCAGCC GGGGTCTGAC CCGGGCCATG 
ACCATGCCCC CAGTGTCACA GCCTGAGGGT TCCATCGTGC TTCGAAGCAA GGATGGCAGA 
GAAAGAGAGC GAGAAAAACG ATTCAGCTTC TTTAAGAAGA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531766.1
CDS	481..7668
Translation	MNTNGVGRVHGNGLNGAAEFYYEAVEGSQNPGGVLLSPAAFINPAQYASVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGVLRLLNKHAALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQQLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVNDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQAGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRKNQEAVQQLLGRLRDNREQQHFLQDCQELRLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANKDWLDKVDKEGRELTLEKPELKVVVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAQALAQEDQSAGEVERTSRAVEEKFRALCQPMKERCRRLHASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYASLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSKDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGQRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMGQELLARSHYAAEEISEKLSQLQSRRQETADKWQEKMDWLQLVLEVLVFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENERKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVPDTAWDGTQSKLPPSTQAPSVNGVCTDTDSSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPAPSPMPQSRSSEAAHGATLPTRGPELSAQEQMEGMLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQAKDEAEMSSWLRVVNAAIATASSAPGESEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKRFSFFKKNK

Target ORF information:

RefSeq Version	XM_006531703.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin beta, non-erythrocytic 2 (Sptbn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531703.3

ORF Insert Sequence:

ATGAATACAA ACGGAGTGGG CAGGGTCCAT GGGAACGGCC TGAACGGGGC TGCTGAGTTT 
TACTATGAGG CTGTGGAAGG GTCTCAGAAC CCTGGGGGCG TCCTGCTCTC ACCAGCTGCC 
TTCATCAATC CTGCTCAGTA TGCCAGTGTG CTGGAAGGAC GCTTCAAACA GCTTCAAGAT 
GAGCGAGAAG CCGTGCAGAA GAAAACCTTC ACCAAGTGGG TGAACTCCCA CCTGGCCCGG 
GTGACATGCC GGGTGGGAGA CCTGTACAGC GACCTGCGGG ACGGGCGCAA CCTCCTGAGG 
CTCCTGGAGG TGCTCTCGGG AGAGACCCTG CCAAAACCTA CCAAGGGCCG AATGCGGATT 
CACTGCCTGG AGAATGTAGA TAAAGCGCTG CAGTTCCTGA AGGAGCAGAA GGTGCACTTG 
GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GTCTGACCCT TGGGCTAGTG 
TGGACCATCA TCCTCCGATT TCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG 
GAGAAGAAGT CAGCCAAGGA TGCCCTGCTG CTGTGGTGCC AGATGAAGAC TGCAGGATAC 
CCCAATGTCA ATGTGCACAA CTTTACCACC AGTTGGAGAG ATGGGCTGGC CTTTAATGCC 
ATTGTGCACA AACACCGGCC AGACCTGTTG GATTTTGAGT CCCTGAAGAA GTGTAATGCA 
CACTACAATC TGCAGAATGC TTTCAATCTG GCTGAAAAGG AACTTGGCCT GACGAAGCTC 
CTGGATCCTG AAGATGTGAA TGTAGACCAG CCCGACGAGA AGTCTATCAT CACCTACGTG 
GCCACTTACT ACCACTACTT CTCCAAGATG AAGGCCCTGG CTGTGGAAGG CAAAAGAATT 
GGCAAGGTCC TAGACCATGC CATGGAGGCA GAACACCTGG TAGAGAAATA CGAGTCCCTG 
GCCTCTGAAC TGCTGCAGTG GATTGAGCAA ACGATCGTGA CCCTCAATGA CCGACAGCTG 
GCCAACTCCC TGAGTGGAGT CCAGAACCAG CTGCAGTCTT TCAACTCCTA TCGCACTGTG 
GAGAAGCCAC CCAAGTTCAC GGAGAAAGGG AACTTGGAGG TGTTGCTCTT CACCATCCAG 
AGCAAGCTAC GGGCCAACAA CCAGAAGGTC TACACACCCC GGGAAGGCCG GCTCATCTCG 
GACATCAACA AGGCCTGGGA GCGGCTGGAG AAAGCCGAAC ATGAGCGAGA GCTAGCGCTG 
CGCACAGAGC TGATCCGCCA GGAGAAGCTG GAGCAACTGG CTGCTCGCTT CGACCGCAAG 
GCTGCCATGC GGGAGACCTG GCTCAGTGAG AACCAGCGAC TTGTGTCCCA GGACAACTTT 
GGGCTGGAAC TGGCAGCAGT GGAGGCTGCA GTGAGAAAGC ATGAAGCCAT TGAGACAGAC 
ATTGTCGCCT ACAGTGGCCG GGTGCAGGCG GTGGATGCTG TAGCTGCAGA ACTGGCTGCT 
GAGCACTACC ATGACATTAA GCGCATTGCG GCACGTCAGA ACAACGTGGC CCGGCTCTGG 
GACTTCTTAC GGCAAATGGT GGCTGCCCGC CGTGAGAGAC TTCTTCTCAA CCTGGAGCTG 
CAGAAAGTGT TTCAGGACCT GCTCTACCTC ATGGACTGGA TGGCAGAGAT GAAGGGCCGA 
CTGCAGTCTC AGGACTTGGG CAAGCACCTG GCTGGAGTGG AGGACCTCCT GCAGCTACAC 
GAACTGGTGG AGGCGGACAT CGCGGTTCAG GCTGAGAGGG TTCGAGCGGT CAGCGCCTCT 
GCCCTGCGTT TCTGCGACCC AGGGAAAGAG TATAGGCCAT GTGACCCACA GCTGGTGTCA 
GAGAGGGTGG CCACTCTGGA GCAGAGCTAT GAGGCCCTGT GTGAATTGGC AGCGACTAGA 
AGGGCCCGAC TGGAAGAGTC ACGTCGTCTC TGGAGGTTCC TCTGGGAAGT GGGTGAGGCT 
GAGGCCTGGG TCCGGGAGCA GCAGCACCTC CTGGCCTCAG CTGATACAGG CCGGGACCTG 
ACTGGTGTCC TTCGCCTGCT CAACAAGCAC GCAGCCCTAC GGGGTGAGAT GAGTGGCCGT 
CTGGGGCCCC TGAAGCTCAC CCTGGAGCAA GGTCAGCAGT TAGTCGCTGA GGGCCACCCT 
GGAGCCAACC AAGCCTCAAC CCGTGCAGCC GAGCTCCAGG CCCAGTGGGA GCGACTAGAA 
GCCCTGGCCG AGGAGCGAGC CCAGCAGCTG GCACAGGCTG CCAGCCTCTA CCAGTTCCAG 
GCAGATGCAA ATGACATGGA GGCCTGGTTG GTGGATGCAC TGCGCCTGGT ATCTAGCCCC 
GAGGTAGGGC ACGACGAGTT CTCCACACAG GCCCTGGCCA GGCAGCACAG GGCCCTTGAA 
GAGGAGATCC GAGCCCACCG GCCTACACTG GATGCCCTGA GGGAGCAGGC TGCAGCCCTG 
CCACCTGCAC TGAGCCACAC ACCGGAGGTA CAGGGCAGGG TGCCCACTCT GGAGCAGCAC 
TATGAGGAGC TGCAGGCCCG AGCCGGTGAG CGTGCACGAG CCCTGGAGGC AGCCCTGGCG 
TTCTATACCA TGCTCAGCGA GGCTGGGGCC TGTGGCCTCT GGGTAGAGGA GAAGGAGCAA 
TGGCTCAACG GGCTGGCCCT ACCTGAGCGC CTGGAGGACC TGGAGGTGGT CCAGCAGAGG 
TTTGAGACCC TGGAGCCTGA AATGAACGCC CTGGCTGCAC GGGTTACTGC TGTCAATGAC 
ATCGCTGAGC AGCTGCTGAA GGCCAGTCCA CCAGGCAAGG ACCGCATCAT TGGCACCCAG 
GAGCAGCTCA ACCAAAGGTG GCAGCAGTTT CGGTCCCTGG CAGATGGCAA AAAGGCGGCT 
CTGACATCAG CCCTGAGCAT CCAGAATTAC CACCTAGAGT GCACAGAGAC CCAGGCCTGG 
ATGAGAGAGA AGACTAAGGT CATTGAGTCC ACCCAGGGCC TGGGCAATGA TCTAGCTGGT 
GTGCTGGCCC TGCAGCGGAA GTTGGCGGGT ACTGAGAGAG ATCTGGAAGC TATCTCTGCC 
CGGGTGGGTG AGCTGACCCA AGAGGCAAAT GCCTTGGCTG CTGGGCACCC AGCTCAAGCT 
CCTGCCATCA ACACCCGGCT TGGAGAGGTA CAAGCAGGAT GGGAAGATCT TCGGGCGACC 
ATGAGGCGGA GAGAAGAGTC CCTGGGTGAG GCTCGGCGGC TGCAGGATTT CCTGCGCAGC 
TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACACAGACCG CTGTAGCCTC CGAAGAAGGG 
CCAGCCACCC TTCCCGAGGC AGAAGCCCTC TTAGCCCAGC ACGCAGCTCT GCGGGGAGAA 
GTGGAGAGAG CCCAGAGCGA GTACAGCCGC CTCAGGACCT TGGGCGAGGA GGTGACCAGA 
GACCAGGCTG ATCCCCAGTG CCTCTTCCTC AGACAGAGGC TGGAAGCCCT TGGAACCGGC 
TGGGAGGAGC TGGGTCGCAT GTGGGAGAGC CGGCAAGGCC GCCTGGCCCA GGCCCATGGC 
TTCCAGGGGT TTTTAAGAGA TGCTCGCCAG GCTGAGGGTG TTCTCAGCAG CCAGGAATAT 
GTCTTGTCTC ACACGGAGAT GCCAGGGACA CTGCAGGCGG CAGATGCAGC CATTAAAAAG 
CTGGAAGACT TCATGAGCAC TATGGACGCC AATGGAGAGC GCATCCGCGG ACTCCTGGAG 
GCTGGACGTC AGCTGGTGTC CAAGGGCAAT ATCCATGCTG AGAAGATCCA AGAGAAGGCG 
GACTCCATCG AGAAGAGGCA CAGAAAGAAT CAGGAGGCGG TGCAGCAGCT TTTAGGACGC 
CTTCGGGACA ACCGAGAGCA GCAGCACTTC TTGCAAGACT GTCAGGAGCT GAGACTCTGG 
ATCGATGAGA AGATGCTGAC AGCTCAGGAT GTGTCCTATG ACGAAGCTCG CAACCTGCAC 
ACCAAGTGGC AGAAACACCA GGCATTCATG GCCGAGCTTG CAGCCAACAA GGACTGGCTG 
GACAAAGTGG ACAAGGAAGG GCGGGAGCTG ACTCTTGAAA AGCCAGAACT CAAAGTCGTA 
GTGTCAGAGA AGCTGGAGGA CCTGCACCGG CGCTGGGATG AACTGGAGAC CACTACCCAA 
GCCAAGGCCC GCAGTCTTTT TGATGCCAAC CGGGCAGAGC TATTTGCTCA AAGCTGTTCT 
GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCAGATGA CTATGGCAAA 
GACCTCACCA GTGTCAACAT TCTGCTGAAG AAGCAACAGA TGCTGGAACG AGAGATGGCT 
GTGAGAGAGA AGGAGGTAGA GGCGATACAG GCCCAGGCCC AGGCCCTGGC CCAGGAAGAC 
CAGAGTGCAG GGGAGGTAGA AAGGACCTCA AGGGCTGTGG AGGAGAAGTT CAGGGCCTTG 
TGTCAACCCA TGAAAGAACG CTGCCGGCGC CTGCACGCCT CCCGGGAGCA GCACCAGTTC 
CACCGGGATG TGGAGGATGA GATATTGTGG GTGACTGAGC GGCTTCCCAT GGCCAGCTCT 
CTGGAACATG GCAAGGACTT GCCCAGTGTC CAGCTTCTCA TGAAGAAAAA CCAGACCCTG 
CAGAAGGAGA TCCAGGGCCA TGAGCCCCGG ATTGCAGACC TCAAAGAGAG GCAGCGCACT 
CTGGGAACAG CAGCAGCAGG TCCAGAGCTG GCTGAGCTCC AAGAAATGTG GAAACGCCTG 
AGCCATGAGC TGGAGCTTCG GGGCAAGCGG CTGGAGGAAG CTCTTCGAGC CCAGCAATTC 
TATCGTGATG CTGCAGAGGC CGAGGCTTGG ATGGGTGAGC AGGAGTTACA CATGATGGGC 
CAGGAAAAGG CCAAGGATGA GCTGAGCGCC CAGGCAGAAG TGAAGAAGCA TCAGGTATTG 
GAACAAGCCC TTGCTGACTA TGCCCAGACC ATCAAACAAC TAGCAGCCAG CAGTCAAGAT 
ATGATTGACC ATGAACATCC AGAGAGCACA AGACTAACAA TACGCCAAGC CCAGGTGGAC 
AAGCTGTACG CCAGCCTGAA GGAGCTGGCA GGAGAGCGGC GCGAGCGCCT GCAGGAGCAC 
CTCCGGCTGT GCCAGCTCCG CAGAGAGCTG GATGACCTGG AACAGTGGAT ACAGGAGCGT 
GAAGTTGTGG CAGCCTCCCA TGAACTGGGC CAAGACTATG AGCACGTGAC TATGCTTCGG 
GACAAATTCC GAGAGTTCTC CAAGGACACC AGCACCATTG GCCAGGAGCG CGTAGACAGT 
GCCAATGCAC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACTGTGGCC 
GAGTGGAAGG ACAGTCTCAA TGAGGCCTGG GCTGACCTGC TGGAGCTGCT GGACACAAGA 
GGTCAGGTGC TGGCTGCTGC GTATGAGCTG CAGCGCTTCC TGCATGGAGC CCGCCAAGCC 
CTGGCACGGG TACAGCACAA GCAGCAGCAG CTTCCAGACG GGACTGGCCG TGACCTCAAT 
GCTGCTGAGG CCCTGCAGCG CCGGCACTGT GCCTATGAGC ACGACATCCA AGCCCTCAGC 
ACTCAGGTCC AGCAAGTTCA AGATGATGGC CAAAGGCTAC AGAAGGCCTA TGCTGGAGAC 
AAGGCTGAGG AAATTGGCCG TCACATGCAG GCAGTGGCTG AGGCCTGGGC CCAGCTCCAG 
GGAAGTTCTG CTGCCCGCCG ACAGCTGTTA CTGGATACCA CGGACAAATT CCGATTCTTT 
AAGGCTGTCC GAGAGTTGAT GCTGTGGATG GATGGGATTA ACCTGCAGAT GGATGCCCAG 
GAGCGGCCCC GGGATGTGTC CTCTGCAGAT TTAGTCATCA AAAACCAACA AGGAATCAAA 
GCAGAGATAG AGGCCAGAGC AGACAGGTTC TCCTCCTGCA TTGACATGGG GCAGGAGCTG 
CTGGCCCGGA GCCACTATGC TGCTGAGGAG ATCTCAGAGA AGCTGTCTCA GCTACAGTCC 
CGGCGCCAGG AGACAGCTGA CAAGTGGCAG GAGAAGATGG ACTGGCTACA GCTTGTTTTG 
GAGGTGCTTG TGTTTGGGAG AGATGCAGGG ATGGCGGAGG CCTGGCTGTG CAGTCAGGAG 
CCATTGGTGC GAAGTGCAGA ACTGGGTTGC ACTGTGGACG AAGTAGAGAG CCTCATCAAG 
CGACACGAAG CCTTTCAGAA GTCGGCCGTG GCCTGGGAAG AGCGTTTCAG TGCGCTGGAG 
AAGCTCACTG CGCTGGAAGA GCGGGAAAAT GAGCGGAAAA GGAAGAGGGA GGAGGAGGAA 
CGGAGGAAAC AGCCTCCTAC TTCAGAGCCC ATGGCTAGTC AGCCAGAAGG GAGTCTGGTA 
GATGGCCAGA GAGTTCCTGA CACTGCCTGG GATGGGACTC AGTCAAAATT GCCACCATCC 
ACACAAGCAC CCAGTGTTAA TGGGGTCTGC ACGGACACCG ACTCCTCACA GCCCCTGTTG 
GAACAGCAGA GACTTGAACA GAGCAATGTC CCCGAAGGAC CTGGATCTGG CACAGGAGAC 
GAGTCCAGTG GGCCCCGGGG AGAGAGGCAG ACCCTGCCCC GGGGCCCTGC TCCTTCTCCA 
ATGCCCCAGA GCAGATCATC TGAAGCAGCT CATGGTGCCA CCCTGCCCAC ACGAGGACCT 
GAGCTCTCTG CTCAGGAACA GATGGAAGGG ATGCTGTGCC GCAAACAAGA GATGGAAGCC 
TTCAACAAGA AAGCTGCCAA CAGGTCATGG CAGAATGTGT ACTGTGTACT TCGGCGTGGG 
AGCCTCGGCT TTTACAAGGA TGCCAGGGCA GCTAGTGCAG GAGTGCCCTA CCATGGAGAA 
GTGCCTGTCA GCCTGGCCAG GGCCCAGGGA AGTGTGGCCT TTGATTATCG GAAACGCAAA 
CATGTTTTCA AGCTGGGCTT ACAGGATGGC AAAGAATACC TATTCCAGGC CAAGGACGAG 
GCAGAGATGA GCTCATGGCT GAGAGTGGTG AATGCAGCCA TTGCCACTGC ATCCTCTGCC 
CCTGGAGAGT CAGAAGAGCC AGTGGTGCCC AGTGCCAGCC GGGGTCTGAC CCGGGCCATG 
ACCATGCCCC CAGTGTCACA GCCTGAGGGT TCCATCGTGC TTCGAAGCAA GGATGGCAGA 
GAAAGAGAGC GAGAAAAACG ATTCAGCTTC TTTAAGAAGA ACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?III Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(27)6442-6459(2017 Jul)
Efimova N,Korobova F,Stankewich MC,Moberly AH,Stolz DB,Wang J,Kashina A,Ma M,Svitkina T

Transcription factor Emx2 controls stereociliary bundle orientation of sensory hair cells.
eLife6(2017 Mar)
Jiang T,Kindt K,Wu DK

Posterior cerebellar Purkinje cells in an SCA5/SPARCA1 mouse model are especially vulnerable to the synergistic effect of loss of ?-III spectrin and GLAST.
Human molecular genetics25(20)4448-4461(2016 10)
Perkins EM,Suminaite D,Clarkson YL,Lee SK,Lyndon AR,Rothstein JD,Wyllie DJ,Tanaka K,Jackson M

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Sptbn2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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