Smg7 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Smg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu77091 NM_001359257.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OMu77091 XM_017319981.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OMu85124 XM_030253296.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu85124 XM_030253298.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu08004 NM_001160256.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OMu10690 NM_001005507.2
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $559.30
$799.00
OMu28138 XM_006529450.4
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu28139 XM_006529451.4
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu28140 XM_030253290.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu28141 XM_030253292.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu85123 XM_030253294.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu08009 NM_001160257.2
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu08009 XM_006529452.3
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu28140 XM_017319978.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu28141 XM_017319979.1
Latest version!
Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OMu08009 NM_001160257.1 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu28139 XM_006529451.3 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu28138 XM_006529450.3 Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu77091
    Clone ID Related Accession (Same CDS sequence) NM_001359257.1 , XM_017319981.1
    Accession Version NM_001359257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110038.25 and AC123613.16. On Dec 29, 2017 this sequence version replaced XM_017319981.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.199105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT
    CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT
    CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC
    CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC
    TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA
    GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT
    GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT
    ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA
    GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC
    CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT
    CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC
    CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT
    CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC
    AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG
    GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA
    CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG
    GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT
    CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT
    AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC
    ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT
    GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA
    ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA
    GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT
    TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT
    CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT
    GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT
    AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC
    CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT
    CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC
    CCTCCACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001346186.1
    CDS1217..3628
    Translation

    Target ORF information:

    RefSeq Version NM_001359257.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001359257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT
    CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT
    CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC
    CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC
    TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA
    GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT
    GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT
    ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA
    GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC
    CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT
    CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC
    CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT
    CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC
    AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG
    GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA
    CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG
    GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT
    CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT
    AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC
    ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT
    GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA
    ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA
    GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT
    TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT
    CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT
    GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT
    AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC
    CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT
    CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC
    CCTCCACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77091
    Clone ID Related Accession (Same CDS sequence) NM_001359257.1 , XM_017319981.1
    Accession Version XM_017319981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product protein SMG7 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT
    CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT
    CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC
    CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC
    TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA
    GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT
    GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT
    ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA
    GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC
    CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT
    CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC
    CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT
    CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC
    AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG
    GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA
    CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG
    GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT
    CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT
    AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC
    ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT
    GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA
    ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA
    GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT
    TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT
    CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT
    GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT
    AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC
    CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT
    CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC
    CCTCCACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175470.1
    CDS1255..3666
    Translation

    Target ORF information:

    RefSeq Version XM_017319981.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017319981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTAAAATCCC AGACAGAACT AAGAAAGACT
    CCAGTGTCTG AAGCCAGGAA AACGCCTGTC ACTCAAACTC CAAGTCAAAC GAGTAATTCT
    CAGTTCATCC CCATCCATCA CCCTGGAGCC TTCCCTCCTC TTCCCAGCCG ACCAGGATTC
    CCGCCCCCAA CATATGTTAT CCCCCCTCCT GTGGCATTTT CTATGGGCTC AGGTTACACC
    TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGT CTACAGCTCA CTCTCCAGCA
    GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT ACAGCCAGCA ACGGCCCTCT
    GGACCAGGGC CAATGAACCA GGGACCTCAA CAGTCACAGC CACCTTCCCA GCCACCCCTT
    ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC AATTGCAGGT TCAAGCTCTA
    GCTCAGCAAC AGCAATCCCC TACAAAAGTC ATACCAGCTT TGGGGAAAAG CCCGCCTCAC
    CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA AACAGCTGTG GAATCCCCCT
    CAGGTTCAAA GCCCACTAGG GAAAATTATG CCTGTGAAAC AGTCCTACTA CCTTCAGACC
    CAAGACCCTA TAAAACTGTT TGAGCCGTCA TTGCAACCTC CTGTAATACA GCAGCAGCCT
    CTAGAGAAAA AAATGAAGCC TTTCCCCATG GAGCCATATA ACCATAATCC CTCAGAAGTC
    AAGGTCCCAG AGTTCTACTG GGATTCTTCC TACAGCATGG CCGATAACAG AGCAGTAATG
    GCTCAGCAAC CAAATATGGA CCGCAGGAGC AAACGGTCAC CTGGAGTCTT CCGTCCAGAA
    CAGGATCCTG TGCCCAGGAT GCCATTTGAG GACCCCAAGA GCTCCCCTCT GCTTCCTCCG
    GACCTGTTAA AGAGTCTGGC TGCCTTGGAG GAAGAGGAAG AGCTGATCTT TTCTAACCCT
    CCTGATCTTT ACCCAGCTCT GCTGGGTCCT CTCGCCTCTC TTCCTGGACG AAGCCTCTTT
    AAATCCTTAT TGGAGAAGCC TTCTGAGCTC ATGTCACATT CATCTTCTTT CCTGTCCCTC
    ACGGGGTTCT CTGTCAATCA GGAAAGATAT CCAAACAGCA GTATGTTCAA TGAAGTGTAT
    GGAAAAAACC TGACAACCAG CTCCAAGGCA GAACTGAACC CCTCAGTCGC CTCCCAGGAA
    ACATCACTGT ACTCCCTTTT TGAAGGGACC CCATGGTCTC CATCACTTCC TGCCAGTTCA
    GATCATTCGA CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTGCCCAGT
    TCTCCTCCAA CGCATAACCA TAATTCTGCT CCATTCTCTA ATTTTGGACC CATTGGGACT
    CCAGATAACA GGGATAGGCG GCCTGCAGAT AGGTGGAAAA CTGATAAACC AGCCATGGGT
    GGTTTTGGTG TTGATTATCT CTCAGCAACG TCATCATCTG AGAGCAGTTG GCATCAGGCT
    AGCACTCCGA GTGGCACCTG GACAGGCCAC GGCCCCTCCA TGGAGGATTC CTCTGCTGTC
    CTCATGGAAA GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG GCCCTCCGCT
    CTGGAGCAGT TGTTAATGCA GCAGAAGCAG AAACAGCAGC GGGGACAAGG TGCCATGAAC
    CCTCCACACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85124
    Clone ID Related Accession (Same CDS sequence) XM_030253296.1 , XM_030253298.1
    Accession Version XM_030253296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT
    GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC
    ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA
    AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA
    CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA
    TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG
    TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC
    GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT
    GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA
    TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT
    ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC
    TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT
    GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG
    AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGTG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109156.1
    CDS1255..3804
    Translation

    Target ORF information:

    RefSeq Version XM_030253296.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT
    GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC
    ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA
    AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA
    CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA
    TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG
    TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC
    GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT
    GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA
    TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT
    ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC
    TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT
    GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG
    AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGTG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85124
    Clone ID Related Accession (Same CDS sequence) XM_030253296.1 , XM_030253298.1
    Accession Version XM_030253298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT
    GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC
    ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA
    AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA
    CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA
    TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG
    TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC
    GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT
    GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA
    TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT
    ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC
    TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT
    GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG
    AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGTG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109158.1
    CDS242..2791
    Translation

    Target ORF information:

    RefSeq Version XM_030253298.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TCGGCATCCT GTGCAAATGT CCTCTCCAGA ATGATTCTCA GGAGTCCAAC 
    AATGCCTACC CCCTTCCTGC AGTCAAGGTC TCCATGGACT GGCTAAGACT CCGACCTAGA
    GTCTTTCAAG AAGCAGTGGT GGATGAAAGA CAGTACATTT GGCCCTGGCT AATTTCTCTT
    CTAAATAGTT TCCATCCCCG TGAAGATGAT CTCTCAAATA CTAATGCCAC ACCACTTCCA
    GAGGAGTTTG AATTACAAGG ATTCTTGGCT TTAAGACCTT CTTTCAGGAA CTTGGATTTT
    TCCAAAGGCC ATCAGGGTAT TACAGGAGAC AAAGAGGGTC AACAACGACG AATACGACAG
    CAGCGTTTGA TCTCCATAGG GAAATGGATT GCTGATAACC AGCCACGGCT GATTCAGTGT
    GAAAATGAGG TAGGGAAATT GTTATTTATC ACAGAAATCC CAGAATTAAT ACTAGAAGAC
    CCCAGTGAAG CCAAAGAGAA CCTCATCCTA CAAGAAACAT CTGTGGTAGA GTCACTGGCT
    ACGGATGGGA GCCCAGGACT GAAATCAGTG CTGTCTACAG GCCGAAATCC AAGCAACAGC
    TGTGACTCAG GAGAGAAACC AGTGGTCACC TTTAAAGAGA ACATTAAGCC ACGAGAAGTG
    AACCAAGGAA GAAGCTTTCC TCCCAAAGAG GTGAGAAGGG ACTGTAGCAA AGGCGTAACT
    GTAACTCAGG AGGACGGACA GAAGGACAGC AGCAAGAGGA GAGCTGAGAC CAAGAGATGC
    ACCTTAGGAA AGTTGCAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CGCCTGTCAC TCAAACTCCA
    AGTCAAACGA GTAATTCTCA GTTCATCCCC ATCCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGAC CAGGATTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGTCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA GTCACAGCCA
    CCTTCCCAGC CACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAA
    TTGCAGGTTC AAGCTCTAGC TCAGCAACAG CAATCCCCTA CAAAAGTCAT ACCAGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAAGC CCACTAGGGA AAATTATGCC TGTGAAACAG
    TCCTACTACC TTCAGACCCA AGACCCTATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATACAGC AGCAGCCTCT AGAGAAAAAA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAG TTCTACTGGG ATTCTTCCTA CAGCATGGCC
    GATAACAGAG CAGTAATGGC TCAGCAACCA AATATGGACC GCAGGAGCAA ACGGTCACCT
    GGAGTCTTCC GTCCAGAACA GGATCCTGTG CCCAGGATGC CATTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATCTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGTCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTATTG GAGAAGCCTT CTGAGCTCAT GTCACATTCA
    TCTTCTTTCC TGTCCCTCAC GGGGTTCTCT GTCAATCAGG AAAGATATCC AAACAGCAGT
    ATGTTCAATG AAGTGTATGG AAAAAACCTG ACAACCAGCT CCAAGGCAGA ACTGAACCCC
    TCAGTCGCCT CCCAGGAAAC ATCACTGTAC TCCCTTTTTG AAGGGACCCC ATGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCGACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TGCCCAGTTC TCCTCCAACG CATAACCATA ATTCTGCTCC ATTCTCTAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGCGGC CTGCAGATAG GTGGAAAACT
    GATAAACCAG CCATGGGTGG TTTTGGTGTT GATTATCTCT CAGCAACGTC ATCATCTGAG
    AGCAGTTGGC ATCAGGCTAG CACTCCGAGT GGCACCTGGA CAGGCCACGG CCCCTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGGC CCTCCGCTCT GGAGCAGTTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGTG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08004
    Clone ID Related Accession (Same CDS sequence) NM_001160256.1
    Accession Version NM_001160256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC086651.1, BC082789.1 and AK129101.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC086651.1, SRR1660815.17053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC
    AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC
    CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT
    GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT
    TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA
    GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG
    AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT
    CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA
    TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG
    CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA
    CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA
    CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG
    AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC
    TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT
    ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC
    AGGATGCCAT TTGAGGACCC CAAGAGCTCC CCTCTGCTTC CTCCGGACCT GTTAAAGAGT
    CTGGCTGCCT TGGAGGAAGA GGAAGAGCTG ATCTTTTCTA ACCCTCCTGA TCTTTACCCA
    GCTCTGCTGG GTCCTCTCGC CTCTCTTCCT GGACGAAGCC TCTTTAAATC CTTATTGGAG
    AAGCCTTCTG AGCTCATGTC ACATTCATCT TCTTTCCTGT CCCTCACGGG GTTCTCTGTC
    AATCAGGAAA GATATCCAAA CAGCAGTATG TTCAATGAAG TGTATGGAAA AAACCTGACA
    ACCAGCTCCA AGGCAGAACT GAACCCCTCA GTCGCCTCCC AGGAAACATC ACTGTACTCC
    CTTTTTGAAG GGACCCCATG GTCTCCATCA CTTCCTGCCA GTTCAGATCA TTCGACACCA
    GCCAGCCAGT CTCCTCATTC CTCTAACCCA AGCAGCCTGC CCAGTTCTCC TCCAACGCAT
    AACCATAATT CTGCTCCATT CTCTAATTTT GGACCCATTG GGACTCCAGA TAACAGGGAT
    AGGCGGCCTG CAGATAGGTG GAAAACTGAT AAACCAGCCA TGGGTGGTTT TGGTGTTGAT
    TATCTCTCAG CAACGTCATC ATCTGAGAGC AGTTGGCATC AGGCTAGCAC TCCGAGTGGC
    ACCTGGACAG GCCACGGCCC CTCCATGGAG GATTCCTCTG CTGTCCTCAT GGAAAGCCTA
    AAGTCTATCT GGTCCAGTTC CATGATGCAT CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA
    ATGCAGCAGA AGCAGAAACA GCAGCGGGGA CAAGGTGCCA TGAACCCTCC ACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153728.1
    CDS110..3526
    Translation

    Target ORF information:

    RefSeq Version NM_001160256.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC
    AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC
    CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT
    GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT
    TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA
    GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG
    AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT
    CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA
    TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG
    CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA
    CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA
    CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG
    AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC
    TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT
    ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC
    AGGATGCCAT TTGAGGACCC CAAGAGCTCC CCTCTGCTTC CTCCGGACCT GTTAAAGAGT
    CTGGCTGCCT TGGAGGAAGA GGAAGAGCTG ATCTTTTCTA ACCCTCCTGA TCTTTACCCA
    GCTCTGCTGG GTCCTCTCGC CTCTCTTCCT GGACGAAGCC TCTTTAAATC CTTATTGGAG
    AAGCCTTCTG AGCTCATGTC ACATTCATCT TCTTTCCTGT CCCTCACGGG GTTCTCTGTC
    AATCAGGAAA GATATCCAAA CAGCAGTATG TTCAATGAAG TGTATGGAAA AAACCTGACA
    ACCAGCTCCA AGGCAGAACT GAACCCCTCA GTCGCCTCCC AGGAAACATC ACTGTACTCC
    CTTTTTGAAG GGACCCCATG GTCTCCATCA CTTCCTGCCA GTTCAGATCA TTCGACACCA
    GCCAGCCAGT CTCCTCATTC CTCTAACCCA AGCAGCCTGC CCAGTTCTCC TCCAACGCAT
    AACCATAATT CTGCTCCATT CTCTAATTTT GGACCCATTG GGACTCCAGA TAACAGGGAT
    AGGCGGCCTG CAGATAGGTG GAAAACTGAT AAACCAGCCA TGGGTGGTTT TGGTGTTGAT
    TATCTCTCAG CAACGTCATC ATCTGAGAGC AGTTGGCATC AGGCTAGCAC TCCGAGTGGC
    ACCTGGACAG GCCACGGCCC CTCCATGGAG GATTCCTCTG CTGTCCTCAT GGAAAGCCTA
    AAGTCTATCT GGTCCAGTTC CATGATGCAT CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA
    ATGCAGCAGA AGCAGAAACA GCAGCGGGGA CAAGGTGCCA TGAACCCTCC ACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu10690
    Clone ID Related Accession (Same CDS sequence) NM_001005507.2
    Accession Version NM_001005507.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC086651.1, BC082789.1 and AK129101.1. On May 16, 2009 this sequence version replaced NM_001005507.1. Transcript Variant: This variant (3) differs in the 5' UTR and has several differences in the coding sequence compared to variant 1. The resulting isoform (3) has a distinct N-terminus and the protein is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC082789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC
    GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG
    CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG
    ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT
    TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA
    TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC
    ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA
    GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC
    TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC
    CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT
    CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT
    CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC
    CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG
    ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG
    CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA
    GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA
    ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA
    GAACAGGATC CTGTGCCCAG GATGCCATTT GAGAAATCCT TATTGGAGAA GCCTTCTGAG
    CTCATGTCAC ATTCATCTTC TTTCCTGTCC CTCACGGGGT TCTCTGTCAA TCAGGAAAGA
    TATCCAAACA GCAGTATGTT CAATGAAGTG TATGGAAAAA ACCTGACAAC CAGCTCCAAG
    GCAGAACTGA ACCCCTCAGT CGCCTCCCAG GAAACATCAC TGTACTCCCT TTTTGAAGGG
    ACCCCATGGT CTCCATCACT TCCTGCCAGT TCAGATCATT CGACACCAGC CAGCCAGTCT
    CCTCATTCCT CTAACCCAAG CAGCCTGCCC AGTTCTCCTC CAACGCATAA CCATAATTCT
    GCTCCATTCT CTAATTTTGG ACCCATTGGG ACTCCAGATA ACAGGGATAG GCGGCCTGCA
    GATAGGTGGA AAACTGATAA ACCAGCCATG GGTGGTTTTG GTGTTGATTA TCTCTCAGCA
    ACGTCATCAT CTGAGAGCAG TTGGCATCAG GCTAGCACTC CGAGTGGCAC CTGGACAGGC
    CACGGCCCCT CCATGGAGGA TTCCTCTGCT GTCCTCATGG AAAGCCTAAA GTCTATCTGG
    TCCAGTTCCA TGATGCATCC TGGGCCCTCC GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG
    CAGAAACAGC AGCGGGGACA AGGTGCCATG AACCCTCCAC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005507.1
    CDS110..3514
    Translation

    Target ORF information:

    RefSeq Version NM_001005507.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005507.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC
    GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG
    CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG
    ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT
    TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA
    TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC
    ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA
    GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC
    TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC
    CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT
    CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT
    CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC
    CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG
    ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG
    CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA
    GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA
    ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA
    GAACAGGATC CTGTGCCCAG GATGCCATTT GAGAAATCCT TATTGGAGAA GCCTTCTGAG
    CTCATGTCAC ATTCATCTTC TTTCCTGTCC CTCACGGGGT TCTCTGTCAA TCAGGAAAGA
    TATCCAAACA GCAGTATGTT CAATGAAGTG TATGGAAAAA ACCTGACAAC CAGCTCCAAG
    GCAGAACTGA ACCCCTCAGT CGCCTCCCAG GAAACATCAC TGTACTCCCT TTTTGAAGGG
    ACCCCATGGT CTCCATCACT TCCTGCCAGT TCAGATCATT CGACACCAGC CAGCCAGTCT
    CCTCATTCCT CTAACCCAAG CAGCCTGCCC AGTTCTCCTC CAACGCATAA CCATAATTCT
    GCTCCATTCT CTAATTTTGG ACCCATTGGG ACTCCAGATA ACAGGGATAG GCGGCCTGCA
    GATAGGTGGA AAACTGATAA ACCAGCCATG GGTGGTTTTG GTGTTGATTA TCTCTCAGCA
    ACGTCATCAT CTGAGAGCAG TTGGCATCAG GCTAGCACTC CGAGTGGCAC CTGGACAGGC
    CACGGCCCCT CCATGGAGGA TTCCTCTGCT GTCCTCATGG AAAGCCTAAA GTCTATCTGG
    TCCAGTTCCA TGATGCATCC TGGGCCCTCC GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG
    CAGAAACAGC AGCGGGGACA AGGTGCCATG AACCCTCCAC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28138
    Clone ID Related Accession (Same CDS sequence) XM_006529450.4 , XM_006529450.3
    Accession Version XM_006529450.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529450.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG
    TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA
    AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA
    CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA
    TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG
    TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA
    TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC
    CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA
    CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006529513.1
    CDS707..4285
    Translation

    Target ORF information:

    RefSeq Version XM_006529450.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006529450.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG
    TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA
    AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA
    CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA
    TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG
    TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA
    TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC
    CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA
    CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28139
    Clone ID Related Accession (Same CDS sequence) XM_006529451.3 , XM_006529451.4
    Accession Version XM_006529451.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529451.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC
    GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG
    CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG
    ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT
    TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA
    TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC
    ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA
    GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC
    TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC
    CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT
    CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT
    CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC
    CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG
    ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG
    CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA
    GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA
    ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA
    GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT
    CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC
    CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC
    TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC
    CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG
    TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG
    GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT
    TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC
    AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG
    ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG
    GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG
    GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT
    GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC
    GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG
    AACCCTCCAC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006529514.1
    CDS594..4148
    Translation

    Target ORF information:

    RefSeq Version XM_006529451.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006529451.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC
    GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG
    CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG
    ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT
    TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA
    TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC
    ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA
    GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC
    TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC
    CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT
    CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT
    CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC
    CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG
    ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG
    CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA
    GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA
    ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA
    GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT
    CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC
    CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC
    TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC
    CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG
    TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG
    GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT
    TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC
    AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG
    ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG
    GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG
    GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT
    GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC
    GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG
    AACCCTCCAC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28140
    Clone ID Related Accession (Same CDS sequence) XM_017319978.1 , XM_030253290.1
    Accession Version XM_030253290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA
    TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG
    GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA
    AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA
    TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT
    CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT
    CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA
    GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT
    TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC
    ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC
    ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG
    GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT
    CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109150.1
    CDS706..4134
    Translation

    Target ORF information:

    RefSeq Version XM_030253290.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA
    TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG
    GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA
    AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA
    TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT
    CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT
    CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA
    GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT
    TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC
    ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC
    ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG
    GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT
    CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28141
    Clone ID Related Accession (Same CDS sequence) XM_030253292.1 , XM_017319979.1
    Accession Version XM_030253292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT
    TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG
    GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC
    TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT
    GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT
    AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG
    CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC
    TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG
    ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG
    CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109152.1
    CDS705..3995
    Translation

    Target ORF information:

    RefSeq Version XM_030253292.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT
    TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG
    GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC
    TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT
    GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT
    AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG
    CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC
    TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG
    ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG
    CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85123
    Clone ID Related Accession (Same CDS sequence) XM_030253294.1
    Accession Version XM_030253294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC
    AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC
    CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT
    GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT
    TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA
    GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG
    AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT
    CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA
    TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG
    CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA
    CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA
    CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG
    AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC
    TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT
    ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC
    AGGATGCCAT TTGAGAAATC CTTATTGGAG AAGCCTTCTG AGCTCATGTC ACATTCATCT
    TCTTTCCTGT CCCTCACGGG GTTCTCTGTC AATCAGGAAA GATATCCAAA CAGCAGTATG
    TTCAATGAAG TGTATGGAAA AAACCTGACA ACCAGCTCCA AGGCAGAACT GAACCCCTCA
    GTCGCCTCCC AGGAAACATC ACTGTACTCC CTTTTTGAAG GGACCCCATG GTCTCCATCA
    CTTCCTGCCA GTTCAGATCA TTCGACACCA GCCAGCCAGT CTCCTCATTC CTCTAACCCA
    AGCAGCCTGC CCAGTTCTCC TCCAACGCAT AACCATAATT CTGCTCCATT CTCTAATTTT
    GGACCCATTG GGACTCCAGA TAACAGGGAT AGGCGGCCTG CAGATAGGTG GAAAACTGAT
    AAACCAGCCA TGGGTGGTTT TGGTGTTGAT TATCTCTCAG CAACGTCATC ATCTGAGAGC
    AGTTGGCATC AGGCTAGCAC TCCGAGTGGC ACCTGGACAG GCCACGGCCC CTCCATGGAG
    GATTCCTCTG CTGTCCTCAT GGAAAGCCTA AAGTCTATCT GGTCCAGTTC CATGATGCAT
    CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA ATGCAGCAGA AGCAGAAACA GCAGCGGGGA
    CAAGGTGCCA TGAACCCTCC ACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109154.1
    CDS592..3858
    Translation

    Target ORF information:

    RefSeq Version XM_030253294.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTAAA ATCCCAGACA GAACTAAGAA AGACTCCAGT GTCTGAAGCC
    AGGAAAACGC CTGTCACTCA AACTCCAAGT CAAACGAGTA ATTCTCAGTT CATCCCCATC
    CATCACCCTG GAGCCTTCCC TCCTCTTCCC AGCCGACCAG GATTCCCGCC CCCAACATAT
    GTTATCCCCC CTCCTGTGGC ATTTTCTATG GGCTCAGGTT ACACCTTCCC AGCTGGTGTT
    TCTGTCCCAG GAACCTTTCT TCAGTCTACA GCTCACTCTC CAGCAGGAAA CCAGGTGCAA
    GCTGGGAAAC AGTCCCACAT TCCTTACAGC CAGCAACGGC CCTCTGGACC AGGGCCAATG
    AACCAGGGAC CTCAACAGTC ACAGCCACCT TCCCAGCCAC CCCTTACATC TTTACCAGCT
    CAGCCAACAG CACAGTCTAC AAGCCAATTG CAGGTTCAAG CTCTAGCTCA GCAACAGCAA
    TCCCCTACAA AAGTCATACC AGCTTTGGGG AAAAGCCCGC CTCACCACTC TGGATTCCAG
    CAGTATCAAC AGGCAGATGC CTCCAAACAG CTGTGGAATC CCCCTCAGGT TCAAAGCCCA
    CTAGGGAAAA TTATGCCTGT GAAACAGTCC TACTACCTTC AGACCCAAGA CCCTATAAAA
    CTGTTTGAGC CGTCATTGCA ACCTCCTGTA ATACAGCAGC AGCCTCTAGA GAAAAAAATG
    AAGCCTTTCC CCATGGAGCC ATATAACCAT AATCCCTCAG AAGTCAAGGT CCCAGAGTTC
    TACTGGGATT CTTCCTACAG CATGGCCGAT AACAGAGCAG TAATGGCTCA GCAACCAAAT
    ATGGACCGCA GGAGCAAACG GTCACCTGGA GTCTTCCGTC CAGAACAGGA TCCTGTGCCC
    AGGATGCCAT TTGAGAAATC CTTATTGGAG AAGCCTTCTG AGCTCATGTC ACATTCATCT
    TCTTTCCTGT CCCTCACGGG GTTCTCTGTC AATCAGGAAA GATATCCAAA CAGCAGTATG
    TTCAATGAAG TGTATGGAAA AAACCTGACA ACCAGCTCCA AGGCAGAACT GAACCCCTCA
    GTCGCCTCCC AGGAAACATC ACTGTACTCC CTTTTTGAAG GGACCCCATG GTCTCCATCA
    CTTCCTGCCA GTTCAGATCA TTCGACACCA GCCAGCCAGT CTCCTCATTC CTCTAACCCA
    AGCAGCCTGC CCAGTTCTCC TCCAACGCAT AACCATAATT CTGCTCCATT CTCTAATTTT
    GGACCCATTG GGACTCCAGA TAACAGGGAT AGGCGGCCTG CAGATAGGTG GAAAACTGAT
    AAACCAGCCA TGGGTGGTTT TGGTGTTGAT TATCTCTCAG CAACGTCATC ATCTGAGAGC
    AGTTGGCATC AGGCTAGCAC TCCGAGTGGC ACCTGGACAG GCCACGGCCC CTCCATGGAG
    GATTCCTCTG CTGTCCTCAT GGAAAGCCTA AAGTCTATCT GGTCCAGTTC CATGATGCAT
    CCTGGGCCCT CCGCTCTGGA GCAGTTGTTA ATGCAGCAGA AGCAGAAACA GCAGCGGGGA
    CAAGGTGCCA TGAACCCTCC ACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08009
    Clone ID Related Accession (Same CDS sequence) NM_001160257.1 , NM_001160257.2 , XM_006529452.3
    Accession Version NM_001160257.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-16
    Organism Mus musculus(House mouse)
    Product protein SMG7 isoform 1
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK129101.1, AC110038.25 and BC082789.1. Transcript Variant: This variant (1) represents the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK129101.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC
    CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT
    GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC
    TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA
    GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG
    CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG
    CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153729.1
    CDS76..3516
    Translation

    Target ORF information:

    RefSeq Version NM_001160257.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC
    CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT
    GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC
    TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA
    GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG
    CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG
    CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08009
    Clone ID Related Accession (Same CDS sequence) NM_001160257.1 , NM_001160257.2 , XM_006529452.3
    Accession Version NM_001160257.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110038.25, AK129101.1 and BC082789.1. On or before Dec 29, 2017 this sequence version replaced XM_006529452.3, NM_001160257.1. Transcript Variant: This variant (1) represents the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.209834.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC
    CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT
    GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC
    TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA
    GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG
    CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG
    CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153729.1
    CDS222..3662
    Translation

    Target ORF information:

    RefSeq Version NM_001160257.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001160257.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC
    CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT
    GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC
    TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA
    GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG
    CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG
    CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08009
    Clone ID Related Accession (Same CDS sequence) NM_001160257.1 , NM_001160257.2 , XM_006529452.3
    Accession Version XM_006529452.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product protein SMG7 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC
    CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT
    GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC
    TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA
    GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG
    CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG
    CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006529515.1
    CDS261..3701
    Translation

    Target ORF information:

    RefSeq Version XM_006529452.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006529452.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATCTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGTCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTATT GGAGAAGCCT TCTGAGCTCA TGTCACATTC ATCTTCTTTC
    CTGTCCCTCA CGGGGTTCTC TGTCAATCAG GAAAGATATC CAAACAGCAG TATGTTCAAT
    GAAGTGTATG GAAAAAACCT GACAACCAGC TCCAAGGCAG AACTGAACCC CTCAGTCGCC
    TCCCAGGAAA CATCACTGTA CTCCCTTTTT GAAGGGACCC CATGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCGAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTGCCCAGTT CTCCTCCAAC GCATAACCAT AATTCTGCTC CATTCTCTAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGCGG CCTGCAGATA GGTGGAAAAC TGATAAACCA
    GCCATGGGTG GTTTTGGTGT TGATTATCTC TCAGCAACGT CATCATCTGA GAGCAGTTGG
    CATCAGGCTA GCACTCCGAG TGGCACCTGG ACAGGCCACG GCCCCTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGG
    CCCTCCGCTC TGGAGCAGTT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGT
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28138
    Clone ID Related Accession (Same CDS sequence) XM_006529450.4 , XM_006529450.3
    Accession Version XM_006529450.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG
    TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA
    AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA
    CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA
    TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG
    TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA
    TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC
    CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA
    CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006529513.1
    CDS261..3839
    Translation

    Target ORF information:

    RefSeq Version XM_006529450.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006529450.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG
    TCACATTCAT CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA
    AACAGCAGTA TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA
    CTGAACCCCT CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA
    TTCTCTAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG
    TGGAAAACTG ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA
    TCATCTGAGA GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC
    CCCTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA
    CAGCAGCGGG GACAAGGTGC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28139
    Clone ID Related Accession (Same CDS sequence) XM_006529451.3 , XM_006529451.4
    Accession Version XM_006529451.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC
    GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG
    CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG
    ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT
    TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA
    TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC
    ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA
    GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC
    TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC
    CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT
    CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT
    CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC
    CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG
    ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG
    CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA
    GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA
    ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA
    GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT
    CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC
    CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC
    TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC
    CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG
    TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG
    GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT
    TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC
    AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG
    ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG
    GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG
    GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT
    GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC
    GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG
    AACCCTCCAC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006529514.1
    CDS148..3702
    Translation

    Target ORF information:

    RefSeq Version XM_006529451.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006529451.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGAAATGACA 
    GATTCTAAGC TGGGTCCGGC TGAGGTCTGG ACATCCAGGC AAGCTCTGCA GGACTTGTAC
    CAGAAAATGC TAGTTACTGA TCTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAAGCCAA GAATCGAGCA
    AACCCAAATC GAAGTGAAGT CCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGCGGC
    TTCTATACTC AGTTATTACA GGAACTGTGT ACGGTGTTTA ATGTAGATTT GCCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATTAGC AATAAACAGA CGCACAGCAG CACCATAGTG
    AAGCCACAGT CTAGCTCCTG CTCCTACATT TGCCAACACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAATCA GACTAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCACCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAATT CCCTTTCCCA
    GCTGCCTCCA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGTTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTA
    TACCTGAGTA AGAGTTTGGA AAAGCTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGACTGC TGTTCCAAAA GGCTTTCAAC TCTCAACAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAATG AAACAGAGCA ACACAGTTAC
    AGCCAAGATG AACAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTCGGC
    ATCCTGTGCA AATGTCCTCT CCAGAATGAT TCTCAGGAGT CCAACAATGC CTACCCCCTT
    CCTGCAGTCA AGGTCTCCAT GGACTGGCTA AGACTCCGAC CTAGAGTCTT TCAAGAAGCA
    GTGGTGGATG AAAGACAGTA CATTTGGCCC TGGCTAATTT CTCTTCTAAA TAGTTTCCAT
    CCCCGTGAAG ATGATCTCTC AAATACTAAT GCCACACCAC TTCCAGAGGA GTTTGAATTA
    CAAGGATTCT TGGCTTTAAG ACCTTCTTTC AGGAACTTGG ATTTTTCCAA AGGCCATCAG
    GGTATTACAG GAGACAAAGA GGGTCAACAA CGACGAATAC GACAGCAGCG TTTGATCTCC
    ATAGGGAAAT GGATTGCTGA TAACCAGCCA CGGCTGATTC AGTGTGAAAA TGAGGTAGGG
    AAATTGTTAT TTATCACAGA AATCCCAGAA TTAATACTAG AAGACCCCAG TGAAGCCAAA
    GAGAACCTCA TCCTACAAGA AACATCTGTG GTAGAGTCAC TGGCTACGGA TGGGAGCCCA
    GGACTGAAAT CAGTGCTGTC TACAGGCCGA AATCCAAGCA ACAGCTGTGA CTCAGGAGAG
    AAACCAGTGG TCACCTTTAA AGAGAACATT AAGCCACGAG AAGTGAACCA AGGAAGAAGC
    TTTCCTCCCA AAGAGGTGAG AAGGGACTGT AGCAAAGGCG TAACTGTAAC TCAGGAGGAC
    GGACAGAAGG ACAGCAGCAA GAGGAGAGCT GAGACCAAGA GATGCACCTT AGGAAAGTTG
    CAGGAAACAG GAAAGCAGAG TGTGGCAGTG CAGGTAAAAT CCCAGACAGA ACTAAGAAAG
    ACTCCAGTGT CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT
    TCTCAGTTCA TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA
    TTCCCGCCCC CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC
    ACCTTCCCAG CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA
    GCAGGAAACC AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC
    TCTGGACCAG GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC
    CTTACATCTT TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT
    CTAGCTCAGC AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT
    CACCACTCTG GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC
    CCTCAGGTTC AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG
    ACCCAAGACC CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG
    CCTCTAGAGA AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA
    GTCAAGGTCC CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA
    ATGGCTCAGC AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA
    GAACAGGATC CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT
    CCGGACCTGT TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC
    CCTCCTGATC TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC
    TTTAAATCCT TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC
    CTCACGGGGT TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG
    TATGGAAAAA ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG
    GAAACATCAC TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT
    TCAGATCATT CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC
    AGTTCTCCTC CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG
    ACTCCAGATA ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG
    GGTGGTTTTG GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG
    GCTAGCACTC CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT
    GTCCTCATGG AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC
    GCTCTGGAGC AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG
    AACCCTCCAC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28140
    Clone ID Related Accession (Same CDS sequence) XM_017319978.1 , XM_030253290.1
    Accession Version XM_017319978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA
    TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG
    GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA
    AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA
    TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT
    CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT
    CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA
    GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT
    TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC
    ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC
    ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG
    GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT
    CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175467.1
    CDS85..3513
    Translation

    Target ORF information:

    RefSeq Version XM_017319978.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017319978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TGAGAAGGGA CTGTAGCAAA
    GGCGTAACTG TAACTCAGGA GGACGGACAG AAGGACAGCA GCAAGAGGAG AGCTGAGACC
    AAGAGATGCA CCTTAGGAAA GTTGCAGGAA ACAGGAAAGC AGAGTGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT
    CAAACTCCAA GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGTCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG
    TCACAGCCAC CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAAT TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA
    CCAGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT
    GTGAAACAGT CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC
    AGCATGGCCG ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA
    CGGTCACCTG GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGAAA
    TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT CTTCTTTCCT GTCCCTCACG
    GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA TGTTCAATGA AGTGTATGGA
    AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT CAGTCGCCTC CCAGGAAACA
    TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT CACTTCCTGC CAGTTCAGAT
    CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC CAAGCAGCCT GCCCAGTTCT
    CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT TTGGACCCAT TGGGACTCCA
    GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG ATAAACCAGC CATGGGTGGT
    TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA GCAGTTGGCA TCAGGCTAGC
    ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG AGGATTCCTC TGCTGTCCTC
    ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC ATCCTGGGCC CTCCGCTCTG
    GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG GACAAGGTGC CATGAACCCT
    CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu28141
    Clone ID Related Accession (Same CDS sequence) XM_030253292.1 , XM_017319979.1
    Accession Version XM_017319979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein SMG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT
    TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG
    GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC
    TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT
    GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT
    AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG
    CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC
    TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG
    ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG
    CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175468.1
    CDS85..3375
    Translation

    Target ORF information:

    RefSeq Version XM_017319979.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017319979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGGACCG AAAACTTGAA ATCAGAGGAG CATCTGAAAT CAAGTAATAT TAGGCAGGCA 
    GAAGTCCTGA AGGCTGAAAT GACAGATTCT AAGCTGGGTC CGGCTGAGGT CTGGACATCC
    AGGCAAGCTC TGCAGGACTT GTACCAGAAA ATGCTAGTTA CTGATCTGGA ATACGCTTTA
    GACAAGAAAG TAGAACAGGA TCTCTGGAAT CATGCCTTTA AGAATCAGAT CACAACACTG
    CAAGGCCAAG CCAAGAATCG AGCAAACCCA AATCGAAGTG AAGTCCAGGC AAACCTTTCT
    CTGTTCCTAG AGGCAGCTAG CGGCTTCTAT ACTCAGTTAT TACAGGAACT GTGTACGGTG
    TTTAATGTAG ATTTGCCATG CCGTGTGAAG TCTTCCCAGT TGGGAATTAT TAGCAATAAA
    CAGACGCACA GCAGCACCAT AGTGAAGCCA CAGTCTAGCT CCTGCTCCTA CATTTGCCAA
    CACTGCCTCG TCCACCTTGG AGACATTGCT CGATACAGAA ATCAGACTAG CCAGGCAGAG
    TCCTACTATA GGCATGCAGC TCAGCTTGTC CCCTCCAATG GTCAGCCTTA CAATCAGTTG
    GCTATCTTAG CTTCTTCCAA AGGAGACCAC CTGACCACAA TTTTCTACTA CTGCAGAAGC
    ATTGCTGTGA AATTCCCTTT CCCAGCTGCC TCCACTAATC TACAAAAAGC ACTTTCTAAA
    GCACTGGAAA GCCGGGATGA GTTGAAAACC AAGTGGGGTG TTTCTGACTT CATCAAGGCC
    TTTATTAAAT TCCACGGTCA TGTATACCTG AGTAAGAGTT TGGAAAAGCT GAGCCCTCTT
    CGAGAGAAGT TGGAAGAACA GTTTAAGAGA CTGCTGTTCC AAAAGGCTTT CAACTCTCAA
    CAGTTAGTTC ATGTCACTGT CATTAACCTG TTTCAACTTC ATCATCTTCG TGACTTTAGC
    AATGAAACAG AGCAACACAG TTACAGCCAA GATGAACAGC TGTGTTGGAC ACAGTTGCTG
    GCCCTCTTTA TGTCTTTTCT CGGCATCCTG TGCAAATGTC CTCTCCAGAA TGATTCTCAG
    GAGTCCAACA ATGCCTACCC CCTTCCTGCA GTCAAGGTCT CCATGGACTG GCTAAGACTC
    CGACCTAGAG TCTTTCAAGA AGCAGTGGTG GATGAAAGAC AGTACATTTG GCCCTGGCTA
    ATTTCTCTTC TAAATAGTTT CCATCCCCGT GAAGATGATC TCTCAAATAC TAATGCCACA
    CCACTTCCAG AGGAGTTTGA ATTACAAGGA TTCTTGGCTT TAAGACCTTC TTTCAGGAAC
    TTGGATTTTT CCAAAGGCCA TCAGGGTATT ACAGGAGACA AAGAGGGTCA ACAACGACGA
    ATACGACAGC AGCGTTTGAT CTCCATAGGG AAATGGATTG CTGATAACCA GCCACGGCTG
    ATTCAGTGTG AAAATGAGGT AGGGAAATTG TTATTTATCA CAGAAATCCC AGAATTAATA
    CTAGAAGACC CCAGTGAAGC CAAAGAGAAC CTCATCCTAC AAGAAACATC TGTGGTAGAG
    TCACTGGCTA CGGATGGGAG CCCAGGACTG AAATCAGTGC TGTCTACAGG CCGAAATCCA
    AGCAACAGCT GTGACTCAGG AGAGAAACCA GTGGTCACCT TTAAAGAGAA CATTAAGCCA
    CGAGAAGTGA ACCAAGGAAG AAGCTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGGAAA ACGCCTGTCA CTCAAACTCC AAGTCAAACG
    AGTAATTCTC AGTTCATCCC CATCCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCCGA
    CCAGGATTCC CGCCCCCAAC ATATGTTATC CCCCCTCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGTC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AGTCACAGCC ACCTTCCCAG
    CCACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA ATTGCAGGTT
    CAAGCTCTAG CTCAGCAACA GCAATCCCCT ACAAAAGTCA TACCAGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAAG CCCACTAGGG AAAATTATGC CTGTGAAACA GTCCTACTAC
    CTTCAGACCC AAGACCCTAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATACAG
    CAGCAGCCTC TAGAGAAAAA AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA GTTCTACTGG GATTCTTCCT ACAGCATGGC CGATAACAGA
    GCAGTAATGG CTCAGCAACC AAATATGGAC CGCAGGAGCA AACGGTCACC TGGAGTCTTC
    CGTCCAGAAC AGGATCCTGT GCCCAGGATG CCATTTGAGA AATCCTTATT GGAGAAGCCT
    TCTGAGCTCA TGTCACATTC ATCTTCTTTC CTGTCCCTCA CGGGGTTCTC TGTCAATCAG
    GAAAGATATC CAAACAGCAG TATGTTCAAT GAAGTGTATG GAAAAAACCT GACAACCAGC
    TCCAAGGCAG AACTGAACCC CTCAGTCGCC TCCCAGGAAA CATCACTGTA CTCCCTTTTT
    GAAGGGACCC CATGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCGAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTGCCCAGTT CTCCTCCAAC GCATAACCAT
    AATTCTGCTC CATTCTCTAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGCGG
    CCTGCAGATA GGTGGAAAAC TGATAAACCA GCCATGGGTG GTTTTGGTGT TGATTATCTC
    TCAGCAACGT CATCATCTGA GAGCAGTTGG CATCAGGCTA GCACTCCGAG TGGCACCTGG
    ACAGGCCACG GCCCCTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGG CCCTCCGCTC TGGAGCAGTT GTTAATGCAG
    CAGAAGCAGA AACAGCAGCG GGGACAAGGT GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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