Gene Symbol | Hhat |
Entrez Gene ID | 226861 |
Full Name | hedgehog acyltransferase |
Synonyms | 2810432O22Rik,AI462858,AP-2CRE,Skn,Tg(TFAP2A-cre)1Will |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Hhat cDNA ORF clone
gRNAs
Gene Symbol | Hhat |
Entrez Gene ID | 226861 |
Full Name | hedgehog acyltransferase |
Synonyms | 2810432O22Rik,AI462858,AP-2CRE,Skn,Tg(TFAP2A-cre)1Will |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_017320220.2 | XP_017175709.1 | protein-cysteine N-palmitoyltransferase HHAT isoform X2 |
XM_017320221.2 | XP_017175710.1 | protein-cysteine N-palmitoyltransferase HHAT isoform X3 |
XM_017320220.1 | XP_017175709.1 | protein-cysteine N-palmitoyltransferase HHAT isoform X2 |
XM_017320221.1 | XP_017175710.1 | protein-cysteine N-palmitoyltransferase HHAT isoform X3 |
XM_011238934.3 | XP_011237236.1 | protein-cysteine N-palmitoyltransferase HHAT isoform X1 |
XM_011238934.2 | XP_011237236.1 | protein-cysteine N-palmitoyltransferase HHAT isoform X1 |
NM_144881.4 | NP_659130.2 | protein-cysteine N-palmitoyltransferase HHAT precursor |
Canis lupus familiaris (dog) | HHAT | XP_537140.3 |
Caenorhabditis elegans (roundworm) | hhat-1 | NP_001022489.1 |
Pan troglodytes (chimpanzee) | HHAT | XP_001169380.1 |
LOC712333 | XP_001109445.2 | |
Bos taurus (cattle) | HHAT | XP_002694341.2 |
Mus musculus (house mouse) | Hhat | NP_659130.2 |
Gallus gallus (chicken) | HHAT | XP_001234561.1 |
Drosophila melanogaster (fruit fly) | rasp | NP_523898.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP002602 | XP_312335.5 |
Caenorhabditis elegans (roundworm) | hhat-2 | NP_001255840.1 |
Xenopus tropicalis (tropical clawed frog) | hhat | XP_002934723.2 |
Homo sapiens (human) | HHAT | NP_001164051.1 |
Rattus norvegicus (Norway rat) | Hhat | XP_006250560.1 |
Danio rerio (zebrafish) | hhat | NP_001108362.1 |
Hydronephrosis in the Wnt5a-ablated kidney is caused by an abnormal ureter-bladder connection.
Yun K, Perantoni AO
Differentiation; research in biological diversity941-7(2017 Mar - Apr)
Ventral neural patterning in the absence of a Shh activity gradient from the floorplate.
Iulianella A, Sakai D, Kurosaka H, Trainor PA
Developmental dynamics : an official publication of the American Association of Anatomists247(1)170-184(2018 Jan)
Cranial nerve development requires co-ordinated Shh and canonical Wnt signaling.
Kurosaka H, Trainor PA, Leroux-Berger M, Iulianella A
PloS one10(3)e0120821(2015)
Loss of function mutation in the palmitoyl-transferase HHAT leads to syndromic 46,XY disorder of sex development by impeding Hedgehog protein palmitoylation and signaling.
Callier P, Calvel P, Matevossian A, Makrythanasis P, Bernard P, Kurosaka H, Vannier A, Thauvin-Robinet C, Borel C, Mazaud-Guittot S, Rolland A, Desdoits-Lethimonier C, Guipponi M, Zimmermann C, St?vant I, Kuhne F, Conne B, Santoni F, Lambert S, Huet F, Mugneret F, Jaruzelska J, Faivre L, Wilhelm D, J?gou B, Trainor PA, Resh MD, Antonarakis SE, Nef S
PLoS genetics10(5)e1004340(2014 May)
Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis.
Kurosaka H, Iulianella A, Williams T, Trainor PA
The Journal of clinical investigation124(4)1660-71(2014 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Mutations in Hedgehog acyltransferase (Hhat) perturb Hedgehog signaling, resulting in severe acrania-holoprosencephaly-agnathia craniofacial defects.
Title: Mutations in Hedgehog acyltransferase (Hhat) perturb Hedgehog signaling, resulting in severe acrania-holoprosencephaly-agnathia craniofacial defects.
The following Hhat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hhat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu21722 | |
Clone ID Related Accession (Same CDS sequence) | NM_144881.4 | |
Accession Version | NM_144881.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-20 | |
Organism | Mus musculus(house mouse) | |
Product | protein-cysteine N-palmitoyltransferase HHAT precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY087228.1 and AK028230.1. On May 16, 2009 this sequence version replaced NM_144881.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028230.1, AK172126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGCTGCCCG GGTGGGAACT GACTCTCTGC CTGCTTGTTT CCTTGGGTTT CCACTTCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_659130.2 |
CDS | 280..1779 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGCTGCCCG GGTGGGAACT GACTCTCTGC CTGCTTGTTT CCTTGGGTTT CCACTTCCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu73314 | |
Clone ID Related Accession (Same CDS sequence) | XM_011238934.3 , XM_011238934.2 | |
Accession Version | XM_011238934.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | protein-cysteine N-palmitoyltransferase HHAT isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011237236.1 |
CDS | 109..1635 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu77107 | |
Clone ID Related Accession (Same CDS sequence) | XM_017320220.2 , XM_017320220.1 | |
Accession Version | XM_017320220.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | protein-cysteine N-palmitoyltransferase HHAT isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_017175709.1 |
CDS | 175..1383 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu77107 | |
Clone ID Related Accession (Same CDS sequence) | XM_017320220.2 , XM_017320220.1 | |
Accession Version | XM_017320220.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | protein-cysteine N-palmitoyltransferase HHAT isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_017175709.1 |
CDS | 177..1385 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu77108 | |
Clone ID Related Accession (Same CDS sequence) | XM_017320221.2 , XM_017320221.1 | |
Accession Version | XM_017320221.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | protein-cysteine N-palmitoyltransferase HHAT isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_017175710.1 |
CDS | 81..878 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu77108 | |
Clone ID Related Accession (Same CDS sequence) | XM_017320221.2 , XM_017320221.1 | |
Accession Version | XM_017320221.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | protein-cysteine N-palmitoyltransferase HHAT isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_017175710.1 |
CDS | 81..878 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu73314 | |
Clone ID Related Accession (Same CDS sequence) | XM_011238934.3 , XM_011238934.2 | |
Accession Version | XM_011238934.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | protein-cysteine N-palmitoyltransferase HHAT isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011237236.1 |
CDS | 99..1625 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Hydronephrosis in the Wnt5a-ablated kidney is caused by an abnormal ureter-bladder connection. |
Ventral neural patterning in the absence of a Shh activity gradient from the floorplate. |
Cranial nerve development requires co-ordinated Shh and canonical Wnt signaling. |
Loss of function mutation in the palmitoyl-transferase HHAT leads to syndromic 46,XY disorder of sex development by impeding Hedgehog protein palmitoylation and signaling. |
Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis. |