NANOS3 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	NANOS3
Entrez Gene ID	244551
Full Name	nanos C2HC-type zinc finger 3
Synonyms	nos3
General protein information	Preferred Names nanos C2HC-type zinc finger 3 Names nanos homolog 3
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357361.1	NP_001344290.1	nanos homolog 3 isoform 2
NM_194059.2	NP_918948.1	nanos homolog 3 isoform 1
XM_006530981.2	XP_006531044.1	nanos homolog 3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NANOS3	NP_001092092.1
Pan troglodytes (chimpanzee)	NANOS3	XP_001171060.1
Mus musculus (house mouse)	Nanos3	NP_918948.1
Rattus norvegicus (Norway rat)	Nanos3	NP_001099415.1
Danio rerio (zebrafish)	nanos3	NP_571953.1
	NANOS3	XP_001111100.2
Canis lupus familiaris (dog)	NANOS3	XP_003432960.1

Gene Ontology
Bibliography

Related articles in PubMed

NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes.
Pui HP, Saga Y
Mechanisms of development(2018 Jan)

NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes.
Zhou Z, Kawabe H, Suzuki A, Shinmyozu K, Saga Y
Nature communications815662(2017 Jun)

Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes.
Kabayama Y, Toh H, Katanaya A, Sakurai T, Chuma S, Kuramochi-Miyagawa S, Saga Y, Nakano T, Sasaki H
Nucleic acids research45(9)5387-5398(2017 May)

SHISA6 Confers Resistance to Differentiation-Promoting Wnt/?-Catenin Signaling in Mouse Spermatogenic Stem Cells.
Tokue M, Ikami K, Mizuno S, Takagi C, Miyagi A, Takada R, Noda C, Kitadate Y, Hara K, Mizuguchi H, Sato T, Taketo MM, Sugiyama F, Ogawa T, Kobayashi S, Ueno N, Takahashi S, Takada S, Yoshida S
Stem cell reports8(3)561-575(2017 03)

Transport of the outer dynein arm complex to cilia requires a cytoplasmic protein Lrrc6.
Inaba Y, Shinohara K, Botilde Y, Nabeshima R, Takaoka K, Ajima R, Lamri L, Takeda H, Saga Y, Nakamura T, Hamada H
Genes to cells : devoted to molecular & cellular mechanisms21(7)728-39(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NANOS3 mutation is one possible cause of primary ovarian insufficiency.
Title: A NANOS3 mutation linked to protein degradation causes premature ovarian insufficiency.

Report distinct posttranscriptional regulations in primordial germ cells soon after their specification, for which RNA-binding proteins such as NANOS3 would play critical functions.
Title: Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3.

Study suggests that Nanos3-3'UTR has an essential role in translational control in embryo.
Title: The Nanos3-3'UTR is required for germ cell specific NANOS3 expression in mouse embryos.

detection of the germ cell-specific proteins Nanos2 and Nanos3 as markers of spermatogonia has enabled the clear dissection of complex populations of these cells as Nanos2 was recently shown to be involved in the maintenance of stem cells
Title: The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3.

Results suggest that Nanos3 maintains the germ cell lineage by suppressing both Bax-dependent and Bax-independent apoptotic pathways.
Title: Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways.

The following NANOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NANOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14244	NM_194059.2 Latest version!	Mus musculus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu41443	NM_001357361.1 Latest version!	Mus musculus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu41443	XM_006530981.2 Latest version!	Mus musculus nanos homolog 3 (Drosophila) (Nanos3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NANOS3 ( NM_194059.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_918948.1 Mus musculus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14244
Clone ID Related Accession (Same CDS sequence)	NM_194059.2
Accession Version	NM_194059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Mus musculus(house mouse)
Product	nanos homolog 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159266.3. On Nov 17, 2006 this sequence version replaced NM_194059.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB095973.1, BC119513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACTT TCAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATAAGGAAG AGGAGCTTGA TGTAAGGCTG GATCCCAAAC CAGAGCCCAA GCCAAGTTCA 
GAAAGCCAGC AAGCCAGCAA GGAATCCTCT GCAGCTCCTG AACGCTTATG TTCATTCTGC 
AAACACAATG GCGAGTCCCG TGCCATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATTTT GCGGGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT TTGCCCACTC ACCAGCCAGG GCTACACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
GATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTG TCTATGCAGG TTCCAAAAGT 
GGCAGGAAGC CCCCTGGACC TTCACCCTCG GCCTGCTGTC CCTCCACTAC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_918948.1
CDS	22..558
Translation	MGTFNLWTDYLGLARLVGALHKEEELDVRLDPKPEPKPSSESQQASKESSAAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATQEHAHTRRFCPLTSQGYTSVYCYTTRNSAGKKLTRPDKAKTQDAGHRLGGEAAAGVYAGSKSGRKPPGPSPSACCPSTTA

Target ORF information:

RefSeq Version	NM_194059.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194059.2

ORF Insert Sequence:

ATGGGGACTT TCAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATAAGGAAG AGGAGCTTGA TGTAAGGCTG GATCCCAAAC CAGAGCCCAA GCCAAGTTCA 
GAAAGCCAGC AAGCCAGCAA GGAATCCTCT GCAGCTCCTG AACGCTTATG TTCATTCTGC 
AAACACAATG GCGAGTCCCG TGCCATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATTTT GCGGGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT TTGCCCACTC ACCAGCCAGG GCTACACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
GATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTG TCTATGCAGG TTCCAAAAGT 
GGCAGGAAGC CCCCTGGACC TTCACCCTCG GCCTGCTGTC CCTCCACTAC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NANOS3 ( NM_001357361.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001344290.1 Mus musculus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu41443
Clone ID Related Accession (Same CDS sequence)	NM_001357361.1 , XM_006530981.2
Accession Version	NM_001357361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	nanos homolog 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159266.3. On Oct 26, 2017 this sequence version replaced XM_006530981.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC119514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACTT TCAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATAAGGAAG AGGAGCTTGA TGTAAGGCTG GATCCCAAAC CAGAGCCCAA GCCAAGTTCA 
GAAAGCCAGC AAGCCAGCAA GGAATCCTCT GCAGCTCCTG AACGCTTATG TTCATTCTGC 
AAACACAATG GCGAGTCCCG TGCCATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATTTT GCGGGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT TTGCCCACTC ACCAGCCAGG GCTACACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
GATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTT CCAAAAGTGG CAGGAAGCCC 
CCTGGACCTT CACCCTCGGC CTGCTGTCCC TCCACTACGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344290.1
CDS	22..546
Translation	MGTFNLWTDYLGLARLVGALHKEEELDVRLDPKPEPKPSSESQQASKESSAAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATQEHAHTRRFCPLTSQGYTSVYCYTTRNSAGKKLTRPDKAKTQDAGHRLGGEAAAGSKSGRKPPGPSPSACCPSTTA

Target ORF information:

RefSeq Version	NM_001357361.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357361.1

ORF Insert Sequence:

ATGGGGACTT TCAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATAAGGAAG AGGAGCTTGA TGTAAGGCTG GATCCCAAAC CAGAGCCCAA GCCAAGTTCA 
GAAAGCCAGC AAGCCAGCAA GGAATCCTCT GCAGCTCCTG AACGCTTATG TTCATTCTGC 
AAACACAATG GCGAGTCCCG TGCCATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATTTT GCGGGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT TTGCCCACTC ACCAGCCAGG GCTACACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
GATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTT CCAAAAGTGG CAGGAAGCCC 
CCTGGACCTT CACCCTCGGC CTGCTGTCCC TCCACTACGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NANOS3 ( XM_006530981.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006531044.1 Mus musculus nanos homolog 3 (Drosophila) (Nanos3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu41443
Clone ID Related Accession (Same CDS sequence)	NM_001357361.1 , XM_006530981.2
Accession Version	XM_006530981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	nanos homolog 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACTT TCAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATAAGGAAG AGGAGCTTGA TGTAAGGCTG GATCCCAAAC CAGAGCCCAA GCCAAGTTCA 
GAAAGCCAGC AAGCCAGCAA GGAATCCTCT GCAGCTCCTG AACGCTTATG TTCATTCTGC 
AAACACAATG GCGAGTCCCG TGCCATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATTTT GCGGGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT TTGCCCACTC ACCAGCCAGG GCTACACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
GATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTT CCAAAAGTGG CAGGAAGCCC 
CCTGGACCTT CACCCTCGGC CTGCTGTCCC TCCACTACGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531044.1
CDS	415..939
Translation	MGTFNLWTDYLGLARLVGALHKEEELDVRLDPKPEPKPSSESQQASKESSAAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATQEHAHTRRFCPLTSQGYTSVYCYTTRNSAGKKLTRPDKAKTQDAGHRLGGEAAAGSKSGRKPPGPSPSACCPSTTA

Target ORF information:

RefSeq Version	XM_006530981.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus nanos homolog 3 (Drosophila) (Nanos3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530981.2

ORF Insert Sequence:

ATGGGGACTT TCAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATAAGGAAG AGGAGCTTGA TGTAAGGCTG GATCCCAAAC CAGAGCCCAA GCCAAGTTCA 
GAAAGCCAGC AAGCCAGCAA GGAATCCTCT GCAGCTCCTG AACGCTTATG TTCATTCTGC 
AAACACAATG GCGAGTCCCG TGCCATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATTTT GCGGGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT TTGCCCACTC ACCAGCCAGG GCTACACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
GATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTT CCAAAAGTGG CAGGAAGCCC 
CCTGGACCTT CACCCTCGGC CTGCTGTCCC TCCACTACGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes.
Mechanisms of development(2018 Jan)
Pui HP,Saga Y

NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes.
Nature communications815662(2017 Jun)
Zhou Z,Kawabe H,Suzuki A,Shinmyozu K,Saga Y

Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes.
Nucleic acids research45(9)5387-5398(2017 May)
Kabayama Y,Toh H,Katanaya A,Sakurai T,Chuma S,Kuramochi-Miyagawa S,Saga Y,Nakano T,Sasaki H

SHISA6 Confers Resistance to Differentiation-Promoting Wnt/?-Catenin Signaling in Mouse Spermatogenic Stem Cells.
Stem cell reports8(3)561-575(2017 03)
Tokue M,Ikami K,Mizuno S,Takagi C,Miyagi A,Takada R,Noda C,Kitadate Y,Hara K,Mizuguchi H,Sato T,Taketo MM,Sugiyama F,Ogawa T,Kobayashi S,Ueno N,Takahashi S,Takada S,Yoshida S

Transport of the outer dynein arm complex to cilia requires a cytoplasmic protein Lrrc6.
Genes to cells : devoted to molecular & cellular mechanisms21(7)728-39(2016 Jul)
Inaba Y,Shinohara K,Botilde Y,Nabeshima R,Takaoka K,Ajima R,Lamri L,Takeda H,Saga Y,Nakamura T,Hamada H

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NANOS3 cDNA ORF clone, Mus musculus(House mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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