Nrk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nrk
Entrez Gene ID	27206
Full Name	Nik related kinase
Synonyms	AA407323,AA409510,AI225895,AV168563,AW538185,Nesk
General protein information	Preferred Names Nik related kinase Names nik-related protein kinase NIK-like embryo-specific kinase nck-interacting kinase-like embryo specific kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F1\|X 61.15 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013724.2	NP_038752.2	nik-related protein kinase
XM_006528550.3	XP_006528613.1	nik-related protein kinase isoform X1
XM_006528550.4	XP_006528613.1	nik-related protein kinase isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NRK	NP_001178094.1
Homo sapiens (human)	NRK	NP_940867.2
Pan troglodytes (chimpanzee)	NRK	XP_521203.2
	LOC700137	XP_002808591.1
Mus musculus (house mouse)	Nrk	NP_038752.2
Canis lupus familiaris (dog)	NRK	XP_549167.2
Rattus norvegicus (Norway rat)	Nrk	XP_006257362.1

Gene Ontology
Bibliography

Related articles in PubMed

Nik-related kinase regulates trophoblast proliferation and placental development by modulating AKT phosphorylation.
Morioka Y, Nam JM, Ohashi T
PloS one12(2)e0171503(2017)

Deficiency of X-Linked Protein Kinase Nrk during Pregnancy Triggers Breast Tumor in Mice.
Yanagawa T, Denda K, Inatani T, Fukushima T, Tanaka T, Kumaki N, Inagaki Y, Komada M
The American journal of pathology186(10)2751-60(2016 Oct)

Nrk, an X-linked protein kinase in the germinal center kinase family, is required for placental development and fetoplacental induction of labor.
Denda K, Nakao-Wakabayashi K, Okamoto N, Kitamura N, Ryu JY, Tagawa Y, Ichisaka T, Yamanaka S, Komada M
The Journal of biological chemistry286(33)28802-10(2011 Aug)

Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family.
Nakano K, Kanai-Azuma M, Kanai Y, Moriyama K, Yazaki K, Hayashi Y, Kitamura N
Experimental cell research287(2)219-27(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

regulates trophoblast proliferation and placental development by modulating AKT phosphorylation
Title: Nik-related kinase regulates trophoblast proliferation and placental development by modulating AKT phosphorylation.

The results suggest that Nrk suppresses the excessive proliferation of mammary epithelial cells during pregnancy, and the impairment of this regulatory system leads to frequent occurrence of breast tumor in Nrk mutant mice.
Title: Deficiency of X-Linked Protein Kinase Nrk during Pregnancy Triggers Breast Tumor in Mice.

Nrk, an X-linked protein kinase in the germinal center kinase family, is required for placental development and fetoplacental induction of labor.
Title: Nrk, an X-linked protein kinase in the germinal center kinase family, is required for placental development and fetoplacental induction of labor.

The following Nrk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nrk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu56519	XM_006528550.4 Latest version!	Mus musculus Nik related kinase (Nrk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu09844	NM_013724.2 Latest version!	Mus musculus Nik related kinase (Nrk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu56519	XM_006528550.3	Mus musculus Nik related kinase (Nrk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nrk ( XM_006528550.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528613.1 Mus musculus Nik related kinase (Nrk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56519
Clone ID Related Accession (Same CDS sequence)	XM_006528550.3 , XM_006528550.4
Accession Version	XM_006528550.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nik-related protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528550.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAC CTGGGAGTTG GAGGGACAAG GAGGTGACAG ATCTTGGCCA GTTGCCGGAT 
CCAACTGGAA TATTCTCACT AGATAAAGCC ATTGGTCTTG GTACTTATGG AAGAATCTTT 
TTGGGAATCC ATGAAAAGAC TGGTTCATTG GTGGCTGTTA AAGTAATGAG TGCCAGGAAG 
ACTCCTTTGC CTGAGATAGG AAGACGGGTG AGAGTGAATA AATACCAAAA GTCTGTTGGA 
TGGAGATACA GCGATGAAGA AGAGGATCTT AGGACGGAAC TCAACCTGCT GAGGAAGTAC 
TCTTTCCACA AAAACATTGT GACCTTCTAT GGAGCATTTT TCAAGCTGAA TCCTCCTGGC 
CATCAGCACC AACTTTGGAT GGTAATGGAG CTATGTGCAG CAGGCTCAGT CACTGATGTA 
GTGAGGATGA CAAGAAATCA GAGTTTAAAA GAAGACTGGA TCGCTTATAT CTGTCGAGAA 
ATCCTTCAGG GCTTAGCTCA CCTTCATGCA CACCAAGTAA TTCACCGGGA CATCAAAGGA 
CAAAATGTGC TGCTGACTCA TGATGCTGAA GTCAAAATAG TGGATTTTGG AGTGAGTGCC 
CAAGTGAGCA GAACAAATGG ACGGAGAAAT AGCTTCATTG GTACACCGTA CTGGATGGCC 
CCAGAGGTGA TTCATTGTGA TGAGGACCCA AGGTGTTCCT ATGATTACAG AAGTGATGTG 
TGGTCTGTGG GAATCACTGC TATTGAAATG GCTGAAGGGG CTCCTCCTCT GTGTAAGCTT 
CAGCCTCTGG AAGCCCTTTG TGTTATTCTA CGGGAAGCCG CTCCCAAAGT CAAATCCAGT 
GGATGGTCCC GTAAGTTCCA GAATTTCATG GAAAATTGCA TGATAAAAAA TTTCCTATTT 
CGTCCTACAT CTGGAAACAT GCTTCTTCAC CCATTTGTCC ATGATATAAA AAATGAACGA 
CGTGTTGTGG AGTCCTTGAC AAAACATCTT ACTGGGATCA TTCAAAAGAG AGAGAAAAAA 
GGAATACCTG TGGCCTTTGA AGGAGAAGAA GCTGCTAAGG AACAGTACAT CACAAGGAGA 
TTCAGAGGGC CTTCTTGCAC TCCTGAGCTT CTCAGAGTGC CAACCAGCAG CAGATGCAGA 
CCACTCAGAG TCCTGCATGG AGAGCCCCCT CAGCCAAGGT GGCTACCTGA TCAAGAAGAC 
CCACAGGACC AAGAACTTCA ACAACTGCAG AAGGCAGCCG GAGTGTTCAT GCCTCTACAT 
TCTCAGGACA ATACATCCAA GCTTTTCCCA AAGCAGGTTG AGGTAGCACC CTATCTCCGT 
GGGGCAGCTC AGGTGGTCAT GCCAGTACTG GTACAGGTTG AAGCACCGCC CCAAGTAAGC 
AAAGCAGCCC AGATGCTCAA GTCACTCCCC ACTCAGGACA ACAAAGCTAC TTCTCCAGAG 
GTCCAGGCCC CCGTAGCCGA AGGACAGCAG GCCCAGCATG AGGCCTTAGA GACAGAACAG 
CCTAAAGATT TGGACCAGGT GCCAGAAGAA TTTCAGGGGC AAGACAGGGC ACCTGAACAG 
CCAAGGCAGG GCCAGGCTGC TGAGCAGCAG CAGATACATA ATCCGGTTCC TGAGCAGCCG 
CCGGAGGAGG ATAGGGAGCC TGAGCAAGCT GAGGTGCAGG AAGAGGCTGT GGAGCCTCCC 
CAAGCTGAGA TCGAGGACAA GGAACCTGAG GTAGTGCAAG TGCACGCCCA GGTGCTCCTG 
CCTTTACTGT CCCAGAACCG TCACGTGCTC TTGCCTCTTC ATTTGGATAG ACAGCTGCTC 
ATTCCGGTAG GGGAACAAAA TGAAGAGGTA CCACGAGCCC AGGCCTGGGA TCTAGAGGCC 
TCAAGGGCAG TTGGTGCAGT GCAAGCGCTG ATCGAGGGAC TATCCAGGGA CTTGCTTCGA 
GCACCAAATG CATTTGTCAC AAAACCACTT GGTCCGCTGC AGATCTTCTT GGAAAATTTG 
TCTACAGACG GGTTTTACAC TGAGCCGGAA CCGACACAGA AGAAAAAGTC GAAAGTTGCT 
AGTCTAAGGA AAGCAATAGC AAAACGACTA AGACCCAAGA GGTTCAGAGC AAAGGCATTA 
TGGCGACTTG AAGATTTTGA ATTCTCAGAT GTAGAAACCA GCAGGAGACG GCGCCACCGT 
AGATGGGAGG ACATCTTTAA TCAGCATGAA GAGCAACTGA GACGAGTTGA AAATGACAGA 
GAAGATGACT CATCAGACAA TGATGAAGTG TTTCATTCAA TTCAGGCTGA AGTCCAAATA 
GAACCACACG CTGCAAATCC TGCAGGAAAT GAGGTCCATG AGAGATCTGC TCCCATGCCT 
TGCAACCGGA ATCGCACACA TCGTGTGAAA TTTTCTCCAA GTGTTGGTGA GGAAGAGCCT 
TCTTTGGAAG AAGCCCAGCC GCAGCAGCAG CAGCAGCAGC CCATGAACAT CCGACCGAGG 
AATTGCCTAA ATCCTCAGAA TTTTCAAGCT CAATCAGACT CATCTTCTGA GGAGGACAGT 
CCTGTGACTA GGAGGAAGTC CCAGTCATCA CCGCCTTATT CTACTATTGA TCAGAAGCTA 
CTGATTGATA TCCACGTTCC AGATGGATTT AAAGTGGGGA AAATATCACC TCCTGTGTAC 
TTGACAAATG AATGGGTAGG CTATAATGCA CTCTCTGAAA TCTTCTGGGA TGACTGGATA 
ATGCCTACAC GTCCCGCTCG GCCACCTGAA GAAGATGGTG ATTATGTTGA ACTCTATGAT 
GCTGATGCCA ATGCTAATGG TGATGAAGAA GTTGCAAACG GTGCTTATGA AGATCCTCGT 
GATGGTGCCA ATGGCCATGA TGACATGAAT AACCAGCTTG ATCAAGCAAA TGGTTATGAA 
GGCCATGGTG CTGCTGGCTA CAATGGTGGA GACGTTGGTG GTAATCATGG TGCTGCTTTC 
AATGGGCCAA GAGCAAATTA TCCCAGAGCG GGAATCTTGA AAAACGGTCA TAATGATGGA 
CGTGCTCTAA ACAGGGGAGC TTTTGGAGTC TTTGGAGACA ATGCAGCCAG AGCTTTCCAT 
GGAGCTGCCG GTGAAGCAGG TGCAGCCTTT GGAAACCATG GAGCCAATAG GGGCAATGGA 
AGAGGTAACA GAAACAGGGA GGCCAATGGA AGGAATGAAG AGAATGGAGC ATTTGGACGG 
GATCAACATG TATTTCCAGA GTTTGAACAT GAGGAATCAG ATCGTGGAAC TGAGACCTCA 
GATTCAATTG CCTTGGAGAT CACATCCTTT GATGGTGAGC AAAACAGTGG TAGACCAGTA 
TCATCAACAA CAATGGGTTT TCCTATTGGA CGCTCATCTC CTAGAGGTTC TGATTTTGGG 
AGTGACATCT CATATAACAG CCCAATACTA CATGTGTATG AAAAGGATTT CTCATCTGAG 
GTCTACTGTG GTTCTTTGTG GGGAGTCAAT TTGTTGCTGG GCACCCAATC TCATCTGTAT 
CTGATGGACC GAAGTGGAAA GGCAGAAATT GTTAAACTTA TAAAGCGAAG ACCATTCCGG 
CAGATTCAAG TCGTGGAGCA GCTCAATTTG CTGATTACCA TCTCTGGCAA AAAGAACAGA 
CTTCGCGTGT ATCATCTGTC TTGGCTGAGG AACAAAATTC TGAATAATGA TCCAAAGAGT 
AAGAAAAGGC AGAAGGCGAT GAGGAAGAAA GAGGAAGCTT GCAAAGCTAT CGATAAGTTG 
ATTGGCTGTG AACACTTCAG TGTCCTTCAA CATGAAGAAA CAACATACAT TGCGGTTGCT 
GTGAAGTCAT CAATTCACCT CTTTGCATGG GCACCAAAAT CCTTTGATGA AAACACTGCC 
ATTAAAGTAT TATGCATTGA CCAATCAGCA GACTCCGAAG GAGACTACAT GTCCTATGAA 
GCCTATATAC GAATATTGGC AAAAATACAG GCAGCTGATC CAGCGAACCG GTTTAAGAGA 
CCAGATGAGC TCCTTCATTT GCTGAAGCTC AAGGTGTTTC CAACTCGTGA TCTTAAGCCA 
CTGACAGTTG ACCTGGCTGT TGGTTCAGAG AAAACACTGA AGATTTTCTT CAGCTCAGCT 
AATGGATATC ATATCATCGA TGCAGAATCT GAAGTTATGT CTGAGGTGAC CTTGCCAAAT 
AATTCACTGG AAATCATTAT CCCACAGAAT GTCGTCATTT TACCTGATTG CTTGGGACTT 
GGTGTGATGC TCTCCCTCAA TGCTGAAGCT GCCTCCGAGG AAGCAAACGA ACAACTCCTT 
AAGAAGATCC TGGACGTGTG GAAGGACATA CCGTCTTCTG TGGCTTTTGA GTGTACAAAG 
AGAATCACTG GATGGGACCA AAAGGCTATT GAAGTGCGCT CTCTTCAGTC AACTATTCTC 
GAGAATGAGC TGAAGCGCCG CTCAATCAAG AAACTGAGAT TTCTCTGTGC CCGTGGTGAC 
AAGATGTTCT TTGCTTCTAC ACTGAGCAAT GACCACAGCC GGGTTTACCT TATGAGCCTT 
GGAAAACTTG AAGAGCTCCA TAGAAGCTAT GCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528613.1
CDS	522..5060
Translation	MAGPGSWRDKEVTDLGQLPDPTGIFSLDKAIGLGTYGRIFLGIHEKTGSLVAVKVMSARKTPLPEIGRRVRVNKYQKSVGWRYSDEEEDLRTELNLLRKYSFHKNIVTFYGAFFKLNPPGHQHQLWMVMELCAAGSVTDVVRMTRNQSLKEDWIAYICREILQGLAHLHAHQVIHRDIKGQNVLLTHDAEVKIVDFGVSAQVSRTNGRRNSFIGTPYWMAPEVIHCDEDPRCSYDYRSDVWSVGITAIEMAEGAPPLCKLQPLEALCVILREAAPKVKSSGWSRKFQNFMENCMIKNFLFRPTSGNMLLHPFVHDIKNERRVVESLTKHLTGIIQKREKKGIPVAFEGEEAAKEQYITRRFRGPSCTPELLRVPTSSRCRPLRVLHGEPPQPRWLPDQEDPQDQELQQLQKAAGVFMPLHSQDNTSKLFPKQVEVAPYLRGAAQVVMPVLVQVEAPPQVSKAAQMLKSLPTQDNKATSPEVQAPVAEGQQAQHEALETEQPKDLDQVPEEFQGQDRAPEQPRQGQAAEQQQIHNPVPEQPPEEDREPEQAEVQEEAVEPPQAEIEDKEPEVVQVHAQVLLPLLSQNRHVLLPLHLDRQLLIPVGEQNEEVPRAQAWDLEASRAVGAVQALIEGLSRDLLRAPNAFVTKPLGPLQIFLENLSTDGFYTEPEPTQKKKSKVASLRKAIAKRLRPKRFRAKALWRLEDFEFSDVETSRRRRHRRWEDIFNQHEEQLRRVENDREDDSSDNDEVFHSIQAEVQIEPHAANPAGNEVHERSAPMPCNRNRTHRVKFSPSVGEEEPSLEEAQPQQQQQQPMNIRPRNCLNPQNFQAQSDSSSEEDSPVTRRKSQSSPPYSTIDQKLLIDIHVPDGFKVGKISPPVYLTNEWVGYNALSEIFWDDWIMPTRPARPPEEDGDYVELYDADANANGDEEVANGAYEDPRDGANGHDDMNNQLDQANGYEGHGAAGYNGGDVGGNHGAAFNGPRANYPRAGILKNGHNDGRALNRGAFGVFGDNAARAFHGAAGEAGAAFGNHGANRGNGRGNRNREANGRNEENGAFGRDQHVFPEFEHEESDRGTETSDSIALEITSFDGEQNSGRPVSSTTMGFPIGRSSPRGSDFGSDISYNSPILHVYEKDFSSEVYCGSLWGVNLLLGTQSHLYLMDRSGKAEIVKLIKRRPFRQIQVVEQLNLLITISGKKNRLRVYHLSWLRNKILNNDPKSKKRQKAMRKKEEACKAIDKLIGCEHFSVLQHEETTYIAVAVKSSIHLFAWAPKSFDENTAIKVLCIDQSADSEGDYMSYEAYIRILAKIQAADPANRFKRPDELLHLLKLKVFPTRDLKPLTVDLAVGSEKTLKIFFSSANGYHIIDAESEVMSEVTLPNNSLEIIIPQNVVILPDCLGLGVMLSLNAEAASEEANEQLLKKILDVWKDIPSSVAFECTKRITGWDQKAIEVRSLQSTILENELKRRSIKKLRFLCARGDKMFFASTLSNDHSRVYLMSLGKLEELHRSYAV

Target ORF information:

RefSeq Version	XM_006528550.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nik related kinase (Nrk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528550.4

ORF Insert Sequence:

ATGGCAGGAC CTGGGAGTTG GAGGGACAAG GAGGTGACAG ATCTTGGCCA GTTGCCGGAT 
CCAACTGGAA TATTCTCACT AGATAAAGCC ATTGGTCTTG GTACTTATGG AAGAATCTTT 
TTGGGAATCC ATGAAAAGAC TGGTTCATTG GTGGCTGTTA AAGTAATGAG TGCCAGGAAG 
ACTCCTTTGC CTGAGATAGG AAGACGGGTG AGAGTGAATA AATACCAAAA GTCTGTTGGA 
TGGAGATACA GCGATGAAGA AGAGGATCTT AGGACGGAAC TCAACCTGCT GAGGAAGTAC 
TCTTTCCACA AAAACATTGT GACCTTCTAT GGAGCATTTT TCAAGCTGAA TCCTCCTGGC 
CATCAGCACC AACTTTGGAT GGTAATGGAG CTATGTGCAG CAGGCTCAGT CACTGATGTA 
GTGAGGATGA CAAGAAATCA GAGTTTAAAA GAAGACTGGA TCGCTTATAT CTGTCGAGAA 
ATCCTTCAGG GCTTAGCTCA CCTTCATGCA CACCAAGTAA TTCACCGGGA CATCAAAGGA 
CAAAATGTGC TGCTGACTCA TGATGCTGAA GTCAAAATAG TGGATTTTGG AGTGAGTGCC 
CAAGTGAGCA GAACAAATGG ACGGAGAAAT AGCTTCATTG GTACACCGTA CTGGATGGCC 
CCAGAGGTGA TTCATTGTGA TGAGGACCCA AGGTGTTCCT ATGATTACAG AAGTGATGTG 
TGGTCTGTGG GAATCACTGC TATTGAAATG GCTGAAGGGG CTCCTCCTCT GTGTAAGCTT 
CAGCCTCTGG AAGCCCTTTG TGTTATTCTA CGGGAAGCCG CTCCCAAAGT CAAATCCAGT 
GGATGGTCCC GTAAGTTCCA GAATTTCATG GAAAATTGCA TGATAAAAAA TTTCCTATTT 
CGTCCTACAT CTGGAAACAT GCTTCTTCAC CCATTTGTCC ATGATATAAA AAATGAACGA 
CGTGTTGTGG AGTCCTTGAC AAAACATCTT ACTGGGATCA TTCAAAAGAG AGAGAAAAAA 
GGAATACCTG TGGCCTTTGA AGGAGAAGAA GCTGCTAAGG AACAGTACAT CACAAGGAGA 
TTCAGAGGGC CTTCTTGCAC TCCTGAGCTT CTCAGAGTGC CAACCAGCAG CAGATGCAGA 
CCACTCAGAG TCCTGCATGG AGAGCCCCCT CAGCCAAGGT GGCTACCTGA TCAAGAAGAC 
CCACAGGACC AAGAACTTCA ACAACTGCAG AAGGCAGCCG GAGTGTTCAT GCCTCTACAT 
TCTCAGGACA ATACATCCAA GCTTTTCCCA AAGCAGGTTG AGGTAGCACC CTATCTCCGT 
GGGGCAGCTC AGGTGGTCAT GCCAGTACTG GTACAGGTTG AAGCACCGCC CCAAGTAAGC 
AAAGCAGCCC AGATGCTCAA GTCACTCCCC ACTCAGGACA ACAAAGCTAC TTCTCCAGAG 
GTCCAGGCCC CCGTAGCCGA AGGACAGCAG GCCCAGCATG AGGCCTTAGA GACAGAACAG 
CCTAAAGATT TGGACCAGGT GCCAGAAGAA TTTCAGGGGC AAGACAGGGC ACCTGAACAG 
CCAAGGCAGG GCCAGGCTGC TGAGCAGCAG CAGATACATA ATCCGGTTCC TGAGCAGCCG 
CCGGAGGAGG ATAGGGAGCC TGAGCAAGCT GAGGTGCAGG AAGAGGCTGT GGAGCCTCCC 
CAAGCTGAGA TCGAGGACAA GGAACCTGAG GTAGTGCAAG TGCACGCCCA GGTGCTCCTG 
CCTTTACTGT CCCAGAACCG TCACGTGCTC TTGCCTCTTC ATTTGGATAG ACAGCTGCTC 
ATTCCGGTAG GGGAACAAAA TGAAGAGGTA CCACGAGCCC AGGCCTGGGA TCTAGAGGCC 
TCAAGGGCAG TTGGTGCAGT GCAAGCGCTG ATCGAGGGAC TATCCAGGGA CTTGCTTCGA 
GCACCAAATG CATTTGTCAC AAAACCACTT GGTCCGCTGC AGATCTTCTT GGAAAATTTG 
TCTACAGACG GGTTTTACAC TGAGCCGGAA CCGACACAGA AGAAAAAGTC GAAAGTTGCT 
AGTCTAAGGA AAGCAATAGC AAAACGACTA AGACCCAAGA GGTTCAGAGC AAAGGCATTA 
TGGCGACTTG AAGATTTTGA ATTCTCAGAT GTAGAAACCA GCAGGAGACG GCGCCACCGT 
AGATGGGAGG ACATCTTTAA TCAGCATGAA GAGCAACTGA GACGAGTTGA AAATGACAGA 
GAAGATGACT CATCAGACAA TGATGAAGTG TTTCATTCAA TTCAGGCTGA AGTCCAAATA 
GAACCACACG CTGCAAATCC TGCAGGAAAT GAGGTCCATG AGAGATCTGC TCCCATGCCT 
TGCAACCGGA ATCGCACACA TCGTGTGAAA TTTTCTCCAA GTGTTGGTGA GGAAGAGCCT 
TCTTTGGAAG AAGCCCAGCC GCAGCAGCAG CAGCAGCAGC CCATGAACAT CCGACCGAGG 
AATTGCCTAA ATCCTCAGAA TTTTCAAGCT CAATCAGACT CATCTTCTGA GGAGGACAGT 
CCTGTGACTA GGAGGAAGTC CCAGTCATCA CCGCCTTATT CTACTATTGA TCAGAAGCTA 
CTGATTGATA TCCACGTTCC AGATGGATTT AAAGTGGGGA AAATATCACC TCCTGTGTAC 
TTGACAAATG AATGGGTAGG CTATAATGCA CTCTCTGAAA TCTTCTGGGA TGACTGGATA 
ATGCCTACAC GTCCCGCTCG GCCACCTGAA GAAGATGGTG ATTATGTTGA ACTCTATGAT 
GCTGATGCCA ATGCTAATGG TGATGAAGAA GTTGCAAACG GTGCTTATGA AGATCCTCGT 
GATGGTGCCA ATGGCCATGA TGACATGAAT AACCAGCTTG ATCAAGCAAA TGGTTATGAA 
GGCCATGGTG CTGCTGGCTA CAATGGTGGA GACGTTGGTG GTAATCATGG TGCTGCTTTC 
AATGGGCCAA GAGCAAATTA TCCCAGAGCG GGAATCTTGA AAAACGGTCA TAATGATGGA 
CGTGCTCTAA ACAGGGGAGC TTTTGGAGTC TTTGGAGACA ATGCAGCCAG AGCTTTCCAT 
GGAGCTGCCG GTGAAGCAGG TGCAGCCTTT GGAAACCATG GAGCCAATAG GGGCAATGGA 
AGAGGTAACA GAAACAGGGA GGCCAATGGA AGGAATGAAG AGAATGGAGC ATTTGGACGG 
GATCAACATG TATTTCCAGA GTTTGAACAT GAGGAATCAG ATCGTGGAAC TGAGACCTCA 
GATTCAATTG CCTTGGAGAT CACATCCTTT GATGGTGAGC AAAACAGTGG TAGACCAGTA 
TCATCAACAA CAATGGGTTT TCCTATTGGA CGCTCATCTC CTAGAGGTTC TGATTTTGGG 
AGTGACATCT CATATAACAG CCCAATACTA CATGTGTATG AAAAGGATTT CTCATCTGAG 
GTCTACTGTG GTTCTTTGTG GGGAGTCAAT TTGTTGCTGG GCACCCAATC TCATCTGTAT 
CTGATGGACC GAAGTGGAAA GGCAGAAATT GTTAAACTTA TAAAGCGAAG ACCATTCCGG 
CAGATTCAAG TCGTGGAGCA GCTCAATTTG CTGATTACCA TCTCTGGCAA AAAGAACAGA 
CTTCGCGTGT ATCATCTGTC TTGGCTGAGG AACAAAATTC TGAATAATGA TCCAAAGAGT 
AAGAAAAGGC AGAAGGCGAT GAGGAAGAAA GAGGAAGCTT GCAAAGCTAT CGATAAGTTG 
ATTGGCTGTG AACACTTCAG TGTCCTTCAA CATGAAGAAA CAACATACAT TGCGGTTGCT 
GTGAAGTCAT CAATTCACCT CTTTGCATGG GCACCAAAAT CCTTTGATGA AAACACTGCC 
ATTAAAGTAT TATGCATTGA CCAATCAGCA GACTCCGAAG GAGACTACAT GTCCTATGAA 
GCCTATATAC GAATATTGGC AAAAATACAG GCAGCTGATC CAGCGAACCG GTTTAAGAGA 
CCAGATGAGC TCCTTCATTT GCTGAAGCTC AAGGTGTTTC CAACTCGTGA TCTTAAGCCA 
CTGACAGTTG ACCTGGCTGT TGGTTCAGAG AAAACACTGA AGATTTTCTT CAGCTCAGCT 
AATGGATATC ATATCATCGA TGCAGAATCT GAAGTTATGT CTGAGGTGAC CTTGCCAAAT 
AATTCACTGG AAATCATTAT CCCACAGAAT GTCGTCATTT TACCTGATTG CTTGGGACTT 
GGTGTGATGC TCTCCCTCAA TGCTGAAGCT GCCTCCGAGG AAGCAAACGA ACAACTCCTT 
AAGAAGATCC TGGACGTGTG GAAGGACATA CCGTCTTCTG TGGCTTTTGA GTGTACAAAG 
AGAATCACTG GATGGGACCA AAAGGCTATT GAAGTGCGCT CTCTTCAGTC AACTATTCTC 
GAGAATGAGC TGAAGCGCCG CTCAATCAAG AAACTGAGAT TTCTCTGTGC CCGTGGTGAC 
AAGATGTTCT TTGCTTCTAC ACTGAGCAAT GACCACAGCC GGGTTTACCT TATGAGCCTT 
GGAAAACTTG AAGAGCTCCA TAGAAGCTAT GCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrk ( NM_013724.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038752.2 Mus musculus Nik related kinase (Nrk), mRNA.

Price & Availability↑

CloneID	OMu09844
Clone ID Related Accession (Same CDS sequence)	NM_013724.2
Accession Version	NM_013724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	nik-related protein kinase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX324117.16 and BX784027.3. On Mar 21, 2009 this sequence version replaced NM_013724.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC139029.1, AB035267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGAC CTGGGAGTTG GAGGGACAAG GAGGTGACAG ATCTTGGCCA GTTGCCGGAT 
CCAACTGGAA TATTCTCACT AGATAAAGCC ATTGGTCTTG GTACTTATGG AAGAATCTTT 
TTGGGAATCC ATGAAAAGAC TGGTTCATTG GTGGCTGTTA AAGTAATGAG TGCCAGGAAG 
ACTCCTTTGC CTGAGATAGG AAGACGGGTG AGAGTGAATA AATACCAAAA GTCTGTTGGA 
TGGAGATACA GCGATGAAGA AGAGGATCTT AGGACGGAAC TCAACCTGCT GAGGAAGTAC 
TCTTTCCACA AAAACATTGT GACCTTCTAT GGAGCATTTT TCAAGCTGAA TCCTCCTGGC 
CATCAGCACC AACTTTGGAT GGTAATGGAG CTATGTGCAG CAGGCTCAGT CACTGATGTA 
GTGAGGATGA CAAGAAATCA GAGTTTAAAA GAAGACTGGA TCGCTTATAT CTGTCGAGAA 
ATCCTTCAGG GCTTAGCTCA CCTTCATGCA CACCAAGTAA TTCACCGGGA CATCAAAGGA 
CAAAATGTGC TGCTGACTCA TGATGCTGAA GTCAAAATAG TGGATTTTGG AGTGAGTGCC 
CAAGTGAGCA GAACAAATGG ACGGAGAAAT AGCTTCATTG GTACACCGTA CTGGATGGCC 
CCAGAGGTGA TTCATTGTGA TGAGGACCCA AGGTGTTCCT ATGATTACAG AAGTGATGTG 
TGGTCTGTGG GAATCACTGC TATTGAAATG GCTGAAGGGG CTCCTCCTCT GTGTAAGCTT 
CAGCCTCTGG AAGCCCTTTG TGTTATTCTA CGGGAAGCCG CTCCCAAAGT CAAATCCAGT 
GGATGGTCCC GTAAGTTCCA GAATTTCATG GAAAATTGCA TGATAAAAAA TTTCCTATTT 
CGTCCTACAT CTGGAAACAT GCTTCTTCAC CCATTTGTCC ATGATATAAA AAATGAACGA 
CGTGTTGTGG AGTCCTTGAC AAAACATCTT ACTGGGATCA TTCAAAAGAG AGAGAAAAAA 
GGAATACCTG TGGCCTTTGA AGGAGAAGAA GCTGCTAAGG AACAGTACAT CACAAGGAGA 
TTCAGAGGGC CTTCTTGCAC TCCTGAGCTT CTCAGAGTGC CAACCAGCAG CAGATGCAGA 
CCACTCAGAG TCCTGCATGG AGAGCCCCCT CAGCCAAGGT GGCTACCTGA TCAAGAAGAC 
CCACAGGACC AAGAACTTCA ACAACTGCAG AAGGCAGCCG GAGTGTTCAT GCCTCTACAT 
TCTCAGGACA ATACATCCAA GCTTTTCCCA AAGCAGGTTG AGGTAGCACC CTATCTCCGT 
GGGGCAGCTC AGGTGGTCAT GCCAGTACTG GTACAGGTTG AAGCACCGCC CCAAGTAAGC 
AAAGCAGCCC AGATGCTCAA GTCACTCCCC ACTCAGGACA ACAAAGCTAC TTCTCCAGAG 
GTCCAGGCCC CCGTAGCCGA AGGACAGCAG GCCCAGCATG AGGCCTTAGA GACAGAACAG 
CCTAAAGATT TGGACCAGGT GCCAGAAGAA TTTCAGGGGC AAGACAGGGC ACCTGAACAG 
CCAAGGCAGG GCCAGGCTGC TGAGCAGCAG CAGATACATA ATCCGGTTCC TGAGCAGCCG 
CCGGAGGAGG ATAGGGAGCC TGAGCAAGCT GAGGTGCAGG AAGAGGCTGT GGAGCCTCCC 
CAAGCTGAGA TCGAGGACAA GGAACCTGAG GTAGTGCAAG TGCACGCCCA GGTGCTCCTG 
CCTTTACTGT CCCAGAACCG TCACGTGCTC TTGCCTCTTC ATTTGGATAG ACAGCTGCTC 
ATTCCGGTAG GGGAACAAAA TGAAGAGGTA CCACGAGCCC AGGCCTGGGA TCTAGAGGCC 
TCAAGGGCAG TTGGTGCAGT GCAAGCGCTG ATCGAGGGAC TATCCAGGGA CTTGCTTCGA 
GCACCAAATG CATTTGTCAC AAAACCACTT GGTCCGCTGC AGATCTTCTT GGAAAATTTG 
TCTACAGACG GGTTTTACAC TGAGCCGGAA CCGACACAGA AGAAAAAGTC GAAAGTTGCT 
AGTCTAAGGA AAGCAATAGC AAAACGACTA AGACCCAAGA GGTTCAGAGC AAAGGCATTA 
TGGCGACTTG AAGATTTTGA ATTCTCAGAT GTAGAAACCA GCAGGAGACG GCGCCACCGT 
AGATGGGAGG ACATCTTTAA TCAGCATGAA GAGCAACTGA GACGAGTTGA AAATGACAGA 
GAAGATGACT CATCAGACAA TGATGAAGTG TTTCATTCAA TTCAGGCTGA AGTCCAAATA 
GAACCACACG CTGCAAATCC TGCAGGAAAT GAGGTCCATG AGAGATCTGC TCCCATGCCT 
TGCAACCGGA ATCGCACACA TCGTGTGAAA TTTTCTCCAA GTGTTGGTGA GGAAGAGCCT 
TCTTTGGAAG AAGCCCAGCC GCAGCAGCAG CAGCAGCAGC CCATGAACAT CCGACCGAGG 
AATTGCCTAA ATCCTCAGAA TTTTCAAGCT CAATCAGACT CATCTTCTGA GGAGGACAGT 
CCTGTGACTA GGAGGAAGTC CCAGTCATCA CCGCCTTATT CTACTATTGA TCAGAAGCTA 
CTGATTGATA TCCACGTTCC AGATGGATTT AAAGTGGGGA AAATATCACC TCCTGTGTAC 
TTGACAAATG AATGGGTAGG CTATAATGCA CTCTCTGAAA TCTTCTGGGA TGACTGGATA 
ATGCCTACAC GTCCCGCTCG GCCACCTGAA GAAGATGGTG ATTATGTTGA ACTCTATGAT 
GCTGATGCCA ATGCTAATGG TGATGAAGAA GTTGCAAACG GTGCTTATGA AGATCCTCGT 
GATGGTGCCA ATGGCCATGA TGACATGAAT AACCAGCTTG ATCAAGCAAA TGGTTATGAA 
GGCCATGGTG CTGCTGGCTA CAATGGTGGA GACGTTGGTG GTAATCATGG TGCTGCTTTC 
AATGGGCCAA GAGCAAATTA TCCCAGAGCG GGAATCTTGA AAAACGGTCA TAATGATGGA 
CGTGCTCTAA ACAGGGGAGC TTTTGGAGTC TTTGGAGACA ATGCAGCCAG AGCTTTCCAT 
GGAGCTGCCG GTGAAGCAGG TGCAGCCTTT GGAAACCATG GAGCCAATAG GGGCAATGGA 
AGAGGTAACA GAAACAGGGA GGCCAATGGA AGGAATGAAG AGAATGGAGC ATTTGGACGG 
GATCAACATG TATTTCCAGA GTTTGAACAT GAGGAATCAG ATCGTGGAAC TGAGACCTCA 
GATTCAATTG CCTTGGAGAT CACATCCTTT GATGGTGAGC AAAACAGTGG TAGACCAGTA 
TCATCAACAA CAATGGGTTT TCCTATTGGA CGCTCATCTC CTAGAGGTTC TGATTTTGGG 
AGTGACATCT CATATAACAG CCCAATACTA CATGTGTATG AAAAGGATTT CTCATCTGAG 
GTCTACTGTG GTTCTTTGTG GGGAGTCAAT TTGTTGCTGG GCACCCAATC TCATCTGTAT 
CTGATGGACC GAAGTGGAAA GGCAGAAATT GTTAAACTTA TAAAGCGAAG ACCATTCCGG 
CAGATTCAAG TCGTGGAGCA GCTCAATTTG CTGATTACCA TCTCTGGCAA AAAGAACAGA 
CTTCGCGTGT ATCATCTGTC TTGGCTGAGG AACAAAATTC TGAATAATGA TCCAAAGAGT 
AAGAAAAGGC AGAAGGCGAT GAGGAAGAAA GAGGAAGCTT GCAAAGCTAT CGATAAGTTG 
ATTGGCTGTG AACACTTCAG TGTCCTTCAA CATGAAGAAA CAACATACAT TGCGGTTGCT 
GTGAAGTCAT CAATTCACCT CTTTGCATGG GCACCAAAAT CCTTTGATGA AAACACTGCC 
ATTAAAGTGT TTCCAACTCG TGATCTTAAG CCACTGACAG TTGACCTGGC TGTTGGTTCA 
GAGAAAACAC TGAAGATTTT CTTCAGCTCA GCTAATGGAT ATCATATCAT CGATGCAGAA 
TCTGAAGTTA TGTCTGAGGT GACCTTGCCA AATAATAATG TCGTCATTTT ACCTGATTGC 
TTGGGACTTG GTGTGATGCT CTCCCTCAAT GCTGAAGCTG CCTCCGAGGA AGCAAACGAA 
CAACTCCTTA AGAAGATCCT GGACGTGTGG AAGGACATAC CGTCTTCTGT GGCTTTTGAG 
TGTACAAAGA GAATCACTGG ATGGGACCAA AAGGCTATTG AAGTGCGCTC TCTTCAGTCA 
ACTATTCTCG AGAATGAGCT GAAGCGCCGC TCAATCAAGA AACTGAGATT TCTCTGTGCC 
CGTGGTGACA AGATGTTCTT TGCTTCTACA CTGAGCAATG ACCACAGCCG GGTTTACCTT 
ATGAGCCTTG GAAAACTTGA AGAGCTCCAT AGAAGCTATG CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038752.2
CDS	323..4690
Translation	MAGPGSWRDKEVTDLGQLPDPTGIFSLDKAIGLGTYGRIFLGIHEKTGSLVAVKVMSARKTPLPEIGRRVRVNKYQKSVGWRYSDEEEDLRTELNLLRKYSFHKNIVTFYGAFFKLNPPGHQHQLWMVMELCAAGSVTDVVRMTRNQSLKEDWIAYICREILQGLAHLHAHQVIHRDIKGQNVLLTHDAEVKIVDFGVSAQVSRTNGRRNSFIGTPYWMAPEVIHCDEDPRCSYDYRSDVWSVGITAIEMAEGAPPLCKLQPLEALCVILREAAPKVKSSGWSRKFQNFMENCMIKNFLFRPTSGNMLLHPFVHDIKNERRVVESLTKHLTGIIQKREKKGIPVAFEGEEAAKEQYITRRFRGPSCTPELLRVPTSSRCRPLRVLHGEPPQPRWLPDQEDPQDQELQQLQKAAGVFMPLHSQDNTSKLFPKQVEVAPYLRGAAQVVMPVLVQVEAPPQVSKAAQMLKSLPTQDNKATSPEVQAPVAEGQQAQHEALETEQPKDLDQVPEEFQGQDRAPEQPRQGQAAEQQQIHNPVPEQPPEEDREPEQAEVQEEAVEPPQAEIEDKEPEVVQVHAQVLLPLLSQNRHVLLPLHLDRQLLIPVGEQNEEVPRAQAWDLEASRAVGAVQALIEGLSRDLLRAPNAFVTKPLGPLQIFLENLSTDGFYTEPEPTQKKKSKVASLRKAIAKRLRPKRFRAKALWRLEDFEFSDVETSRRRRHRRWEDIFNQHEEQLRRVENDREDDSSDNDEVFHSIQAEVQIEPHAANPAGNEVHERSAPMPCNRNRTHRVKFSPSVGEEEPSLEEAQPQQQQQQPMNIRPRNCLNPQNFQAQSDSSSEEDSPVTRRKSQSSPPYSTIDQKLLIDIHVPDGFKVGKISPPVYLTNEWVGYNALSEIFWDDWIMPTRPARPPEEDGDYVELYDADANANGDEEVANGAYEDPRDGANGHDDMNNQLDQANGYEGHGAAGYNGGDVGGNHGAAFNGPRANYPRAGILKNGHNDGRALNRGAFGVFGDNAARAFHGAAGEAGAAFGNHGANRGNGRGNRNREANGRNEENGAFGRDQHVFPEFEHEESDRGTETSDSIALEITSFDGEQNSGRPVSSTTMGFPIGRSSPRGSDFGSDISYNSPILHVYEKDFSSEVYCGSLWGVNLLLGTQSHLYLMDRSGKAEIVKLIKRRPFRQIQVVEQLNLLITISGKKNRLRVYHLSWLRNKILNNDPKSKKRQKAMRKKEEACKAIDKLIGCEHFSVLQHEETTYIAVAVKSSIHLFAWAPKSFDENTAIKVFPTRDLKPLTVDLAVGSEKTLKIFFSSANGYHIIDAESEVMSEVTLPNNNVVILPDCLGLGVMLSLNAEAASEEANEQLLKKILDVWKDIPSSVAFECTKRITGWDQKAIEVRSLQSTILENELKRRSIKKLRFLCARGDKMFFASTLSNDHSRVYLMSLGKLEELHRSYAV

Target ORF information:

RefSeq Version	NM_013724.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nik related kinase (Nrk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013724.2

ORF Insert Sequence:

ATGGCAGGAC CTGGGAGTTG GAGGGACAAG GAGGTGACAG ATCTTGGCCA GTTGCCGGAT 
CCAACTGGAA TATTCTCACT AGATAAAGCC ATTGGTCTTG GTACTTATGG AAGAATCTTT 
TTGGGAATCC ATGAAAAGAC TGGTTCATTG GTGGCTGTTA AAGTAATGAG TGCCAGGAAG 
ACTCCTTTGC CTGAGATAGG AAGACGGGTG AGAGTGAATA AATACCAAAA GTCTGTTGGA 
TGGAGATACA GCGATGAAGA AGAGGATCTT AGGACGGAAC TCAACCTGCT GAGGAAGTAC 
TCTTTCCACA AAAACATTGT GACCTTCTAT GGAGCATTTT TCAAGCTGAA TCCTCCTGGC 
CATCAGCACC AACTTTGGAT GGTAATGGAG CTATGTGCAG CAGGCTCAGT CACTGATGTA 
GTGAGGATGA CAAGAAATCA GAGTTTAAAA GAAGACTGGA TCGCTTATAT CTGTCGAGAA 
ATCCTTCAGG GCTTAGCTCA CCTTCATGCA CACCAAGTAA TTCACCGGGA CATCAAAGGA 
CAAAATGTGC TGCTGACTCA TGATGCTGAA GTCAAAATAG TGGATTTTGG AGTGAGTGCC 
CAAGTGAGCA GAACAAATGG ACGGAGAAAT AGCTTCATTG GTACACCGTA CTGGATGGCC 
CCAGAGGTGA TTCATTGTGA TGAGGACCCA AGGTGTTCCT ATGATTACAG AAGTGATGTG 
TGGTCTGTGG GAATCACTGC TATTGAAATG GCTGAAGGGG CTCCTCCTCT GTGTAAGCTT 
CAGCCTCTGG AAGCCCTTTG TGTTATTCTA CGGGAAGCCG CTCCCAAAGT CAAATCCAGT 
GGATGGTCCC GTAAGTTCCA GAATTTCATG GAAAATTGCA TGATAAAAAA TTTCCTATTT 
CGTCCTACAT CTGGAAACAT GCTTCTTCAC CCATTTGTCC ATGATATAAA AAATGAACGA 
CGTGTTGTGG AGTCCTTGAC AAAACATCTT ACTGGGATCA TTCAAAAGAG AGAGAAAAAA 
GGAATACCTG TGGCCTTTGA AGGAGAAGAA GCTGCTAAGG AACAGTACAT CACAAGGAGA 
TTCAGAGGGC CTTCTTGCAC TCCTGAGCTT CTCAGAGTGC CAACCAGCAG CAGATGCAGA 
CCACTCAGAG TCCTGCATGG AGAGCCCCCT CAGCCAAGGT GGCTACCTGA TCAAGAAGAC 
CCACAGGACC AAGAACTTCA ACAACTGCAG AAGGCAGCCG GAGTGTTCAT GCCTCTACAT 
TCTCAGGACA ATACATCCAA GCTTTTCCCA AAGCAGGTTG AGGTAGCACC CTATCTCCGT 
GGGGCAGCTC AGGTGGTCAT GCCAGTACTG GTACAGGTTG AAGCACCGCC CCAAGTAAGC 
AAAGCAGCCC AGATGCTCAA GTCACTCCCC ACTCAGGACA ACAAAGCTAC TTCTCCAGAG 
GTCCAGGCCC CCGTAGCCGA AGGACAGCAG GCCCAGCATG AGGCCTTAGA GACAGAACAG 
CCTAAAGATT TGGACCAGGT GCCAGAAGAA TTTCAGGGGC AAGACAGGGC ACCTGAACAG 
CCAAGGCAGG GCCAGGCTGC TGAGCAGCAG CAGATACATA ATCCGGTTCC TGAGCAGCCG 
CCGGAGGAGG ATAGGGAGCC TGAGCAAGCT GAGGTGCAGG AAGAGGCTGT GGAGCCTCCC 
CAAGCTGAGA TCGAGGACAA GGAACCTGAG GTAGTGCAAG TGCACGCCCA GGTGCTCCTG 
CCTTTACTGT CCCAGAACCG TCACGTGCTC TTGCCTCTTC ATTTGGATAG ACAGCTGCTC 
ATTCCGGTAG GGGAACAAAA TGAAGAGGTA CCACGAGCCC AGGCCTGGGA TCTAGAGGCC 
TCAAGGGCAG TTGGTGCAGT GCAAGCGCTG ATCGAGGGAC TATCCAGGGA CTTGCTTCGA 
GCACCAAATG CATTTGTCAC AAAACCACTT GGTCCGCTGC AGATCTTCTT GGAAAATTTG 
TCTACAGACG GGTTTTACAC TGAGCCGGAA CCGACACAGA AGAAAAAGTC GAAAGTTGCT 
AGTCTAAGGA AAGCAATAGC AAAACGACTA AGACCCAAGA GGTTCAGAGC AAAGGCATTA 
TGGCGACTTG AAGATTTTGA ATTCTCAGAT GTAGAAACCA GCAGGAGACG GCGCCACCGT 
AGATGGGAGG ACATCTTTAA TCAGCATGAA GAGCAACTGA GACGAGTTGA AAATGACAGA 
GAAGATGACT CATCAGACAA TGATGAAGTG TTTCATTCAA TTCAGGCTGA AGTCCAAATA 
GAACCACACG CTGCAAATCC TGCAGGAAAT GAGGTCCATG AGAGATCTGC TCCCATGCCT 
TGCAACCGGA ATCGCACACA TCGTGTGAAA TTTTCTCCAA GTGTTGGTGA GGAAGAGCCT 
TCTTTGGAAG AAGCCCAGCC GCAGCAGCAG CAGCAGCAGC CCATGAACAT CCGACCGAGG 
AATTGCCTAA ATCCTCAGAA TTTTCAAGCT CAATCAGACT CATCTTCTGA GGAGGACAGT 
CCTGTGACTA GGAGGAAGTC CCAGTCATCA CCGCCTTATT CTACTATTGA TCAGAAGCTA 
CTGATTGATA TCCACGTTCC AGATGGATTT AAAGTGGGGA AAATATCACC TCCTGTGTAC 
TTGACAAATG AATGGGTAGG CTATAATGCA CTCTCTGAAA TCTTCTGGGA TGACTGGATA 
ATGCCTACAC GTCCCGCTCG GCCACCTGAA GAAGATGGTG ATTATGTTGA ACTCTATGAT 
GCTGATGCCA ATGCTAATGG TGATGAAGAA GTTGCAAACG GTGCTTATGA AGATCCTCGT 
GATGGTGCCA ATGGCCATGA TGACATGAAT AACCAGCTTG ATCAAGCAAA TGGTTATGAA 
GGCCATGGTG CTGCTGGCTA CAATGGTGGA GACGTTGGTG GTAATCATGG TGCTGCTTTC 
AATGGGCCAA GAGCAAATTA TCCCAGAGCG GGAATCTTGA AAAACGGTCA TAATGATGGA 
CGTGCTCTAA ACAGGGGAGC TTTTGGAGTC TTTGGAGACA ATGCAGCCAG AGCTTTCCAT 
GGAGCTGCCG GTGAAGCAGG TGCAGCCTTT GGAAACCATG GAGCCAATAG GGGCAATGGA 
AGAGGTAACA GAAACAGGGA GGCCAATGGA AGGAATGAAG AGAATGGAGC ATTTGGACGG 
GATCAACATG TATTTCCAGA GTTTGAACAT GAGGAATCAG ATCGTGGAAC TGAGACCTCA 
GATTCAATTG CCTTGGAGAT CACATCCTTT GATGGTGAGC AAAACAGTGG TAGACCAGTA 
TCATCAACAA CAATGGGTTT TCCTATTGGA CGCTCATCTC CTAGAGGTTC TGATTTTGGG 
AGTGACATCT CATATAACAG CCCAATACTA CATGTGTATG AAAAGGATTT CTCATCTGAG 
GTCTACTGTG GTTCTTTGTG GGGAGTCAAT TTGTTGCTGG GCACCCAATC TCATCTGTAT 
CTGATGGACC GAAGTGGAAA GGCAGAAATT GTTAAACTTA TAAAGCGAAG ACCATTCCGG 
CAGATTCAAG TCGTGGAGCA GCTCAATTTG CTGATTACCA TCTCTGGCAA AAAGAACAGA 
CTTCGCGTGT ATCATCTGTC TTGGCTGAGG AACAAAATTC TGAATAATGA TCCAAAGAGT 
AAGAAAAGGC AGAAGGCGAT GAGGAAGAAA GAGGAAGCTT GCAAAGCTAT CGATAAGTTG 
ATTGGCTGTG AACACTTCAG TGTCCTTCAA CATGAAGAAA CAACATACAT TGCGGTTGCT 
GTGAAGTCAT CAATTCACCT CTTTGCATGG GCACCAAAAT CCTTTGATGA AAACACTGCC 
ATTAAAGTGT TTCCAACTCG TGATCTTAAG CCACTGACAG TTGACCTGGC TGTTGGTTCA 
GAGAAAACAC TGAAGATTTT CTTCAGCTCA GCTAATGGAT ATCATATCAT CGATGCAGAA 
TCTGAAGTTA TGTCTGAGGT GACCTTGCCA AATAATAATG TCGTCATTTT ACCTGATTGC 
TTGGGACTTG GTGTGATGCT CTCCCTCAAT GCTGAAGCTG CCTCCGAGGA AGCAAACGAA 
CAACTCCTTA AGAAGATCCT GGACGTGTGG AAGGACATAC CGTCTTCTGT GGCTTTTGAG 
TGTACAAAGA GAATCACTGG ATGGGACCAA AAGGCTATTG AAGTGCGCTC TCTTCAGTCA 
ACTATTCTCG AGAATGAGCT GAAGCGCCGC TCAATCAAGA AACTGAGATT TCTCTGTGCC 
CGTGGTGACA AGATGTTCTT TGCTTCTACA CTGAGCAATG ACCACAGCCG GGTTTACCTT 
ATGAGCCTTG GAAAACTTGA AGAGCTCCAT AGAAGCTATG CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nrk ( XM_006528550.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528613.1 Mus musculus Nik related kinase (Nrk), transcript variant X1, mRNA.

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CloneID	OMu56519
Clone ID Related Accession (Same CDS sequence)	XM_006528550.3 , XM_006528550.4
Accession Version	XM_006528550.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nik-related protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528550.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAC CTGGGAGTTG GAGGGACAAG GAGGTGACAG ATCTTGGCCA GTTGCCGGAT 
CCAACTGGAA TATTCTCACT AGATAAAGCC ATTGGTCTTG GTACTTATGG AAGAATCTTT 
TTGGGAATCC ATGAAAAGAC TGGTTCATTG GTGGCTGTTA AAGTAATGAG TGCCAGGAAG 
ACTCCTTTGC CTGAGATAGG AAGACGGGTG AGAGTGAATA AATACCAAAA GTCTGTTGGA 
TGGAGATACA GCGATGAAGA AGAGGATCTT AGGACGGAAC TCAACCTGCT GAGGAAGTAC 
TCTTTCCACA AAAACATTGT GACCTTCTAT GGAGCATTTT TCAAGCTGAA TCCTCCTGGC 
CATCAGCACC AACTTTGGAT GGTAATGGAG CTATGTGCAG CAGGCTCAGT CACTGATGTA 
GTGAGGATGA CAAGAAATCA GAGTTTAAAA GAAGACTGGA TCGCTTATAT CTGTCGAGAA 
ATCCTTCAGG GCTTAGCTCA CCTTCATGCA CACCAAGTAA TTCACCGGGA CATCAAAGGA 
CAAAATGTGC TGCTGACTCA TGATGCTGAA GTCAAAATAG TGGATTTTGG AGTGAGTGCC 
CAAGTGAGCA GAACAAATGG ACGGAGAAAT AGCTTCATTG GTACACCGTA CTGGATGGCC 
CCAGAGGTGA TTCATTGTGA TGAGGACCCA AGGTGTTCCT ATGATTACAG AAGTGATGTG 
TGGTCTGTGG GAATCACTGC TATTGAAATG GCTGAAGGGG CTCCTCCTCT GTGTAAGCTT 
CAGCCTCTGG AAGCCCTTTG TGTTATTCTA CGGGAAGCCG CTCCCAAAGT CAAATCCAGT 
GGATGGTCCC GTAAGTTCCA GAATTTCATG GAAAATTGCA TGATAAAAAA TTTCCTATTT 
CGTCCTACAT CTGGAAACAT GCTTCTTCAC CCATTTGTCC ATGATATAAA AAATGAACGA 
CGTGTTGTGG AGTCCTTGAC AAAACATCTT ACTGGGATCA TTCAAAAGAG AGAGAAAAAA 
GGAATACCTG TGGCCTTTGA AGGAGAAGAA GCTGCTAAGG AACAGTACAT CACAAGGAGA 
TTCAGAGGGC CTTCTTGCAC TCCTGAGCTT CTCAGAGTGC CAACCAGCAG CAGATGCAGA 
CCACTCAGAG TCCTGCATGG AGAGCCCCCT CAGCCAAGGT GGCTACCTGA TCAAGAAGAC 
CCACAGGACC AAGAACTTCA ACAACTGCAG AAGGCAGCCG GAGTGTTCAT GCCTCTACAT 
TCTCAGGACA ATACATCCAA GCTTTTCCCA AAGCAGGTTG AGGTAGCACC CTATCTCCGT 
GGGGCAGCTC AGGTGGTCAT GCCAGTACTG GTACAGGTTG AAGCACCGCC CCAAGTAAGC 
AAAGCAGCCC AGATGCTCAA GTCACTCCCC ACTCAGGACA ACAAAGCTAC TTCTCCAGAG 
GTCCAGGCCC CCGTAGCCGA AGGACAGCAG GCCCAGCATG AGGCCTTAGA GACAGAACAG 
CCTAAAGATT TGGACCAGGT GCCAGAAGAA TTTCAGGGGC AAGACAGGGC ACCTGAACAG 
CCAAGGCAGG GCCAGGCTGC TGAGCAGCAG CAGATACATA ATCCGGTTCC TGAGCAGCCG 
CCGGAGGAGG ATAGGGAGCC TGAGCAAGCT GAGGTGCAGG AAGAGGCTGT GGAGCCTCCC 
CAAGCTGAGA TCGAGGACAA GGAACCTGAG GTAGTGCAAG TGCACGCCCA GGTGCTCCTG 
CCTTTACTGT CCCAGAACCG TCACGTGCTC TTGCCTCTTC ATTTGGATAG ACAGCTGCTC 
ATTCCGGTAG GGGAACAAAA TGAAGAGGTA CCACGAGCCC AGGCCTGGGA TCTAGAGGCC 
TCAAGGGCAG TTGGTGCAGT GCAAGCGCTG ATCGAGGGAC TATCCAGGGA CTTGCTTCGA 
GCACCAAATG CATTTGTCAC AAAACCACTT GGTCCGCTGC AGATCTTCTT GGAAAATTTG 
TCTACAGACG GGTTTTACAC TGAGCCGGAA CCGACACAGA AGAAAAAGTC GAAAGTTGCT 
AGTCTAAGGA AAGCAATAGC AAAACGACTA AGACCCAAGA GGTTCAGAGC AAAGGCATTA 
TGGCGACTTG AAGATTTTGA ATTCTCAGAT GTAGAAACCA GCAGGAGACG GCGCCACCGT 
AGATGGGAGG ACATCTTTAA TCAGCATGAA GAGCAACTGA GACGAGTTGA AAATGACAGA 
GAAGATGACT CATCAGACAA TGATGAAGTG TTTCATTCAA TTCAGGCTGA AGTCCAAATA 
GAACCACACG CTGCAAATCC TGCAGGAAAT GAGGTCCATG AGAGATCTGC TCCCATGCCT 
TGCAACCGGA ATCGCACACA TCGTGTGAAA TTTTCTCCAA GTGTTGGTGA GGAAGAGCCT 
TCTTTGGAAG AAGCCCAGCC GCAGCAGCAG CAGCAGCAGC CCATGAACAT CCGACCGAGG 
AATTGCCTAA ATCCTCAGAA TTTTCAAGCT CAATCAGACT CATCTTCTGA GGAGGACAGT 
CCTGTGACTA GGAGGAAGTC CCAGTCATCA CCGCCTTATT CTACTATTGA TCAGAAGCTA 
CTGATTGATA TCCACGTTCC AGATGGATTT AAAGTGGGGA AAATATCACC TCCTGTGTAC 
TTGACAAATG AATGGGTAGG CTATAATGCA CTCTCTGAAA TCTTCTGGGA TGACTGGATA 
ATGCCTACAC GTCCCGCTCG GCCACCTGAA GAAGATGGTG ATTATGTTGA ACTCTATGAT 
GCTGATGCCA ATGCTAATGG TGATGAAGAA GTTGCAAACG GTGCTTATGA AGATCCTCGT 
GATGGTGCCA ATGGCCATGA TGACATGAAT AACCAGCTTG ATCAAGCAAA TGGTTATGAA 
GGCCATGGTG CTGCTGGCTA CAATGGTGGA GACGTTGGTG GTAATCATGG TGCTGCTTTC 
AATGGGCCAA GAGCAAATTA TCCCAGAGCG GGAATCTTGA AAAACGGTCA TAATGATGGA 
CGTGCTCTAA ACAGGGGAGC TTTTGGAGTC TTTGGAGACA ATGCAGCCAG AGCTTTCCAT 
GGAGCTGCCG GTGAAGCAGG TGCAGCCTTT GGAAACCATG GAGCCAATAG GGGCAATGGA 
AGAGGTAACA GAAACAGGGA GGCCAATGGA AGGAATGAAG AGAATGGAGC ATTTGGACGG 
GATCAACATG TATTTCCAGA GTTTGAACAT GAGGAATCAG ATCGTGGAAC TGAGACCTCA 
GATTCAATTG CCTTGGAGAT CACATCCTTT GATGGTGAGC AAAACAGTGG TAGACCAGTA 
TCATCAACAA CAATGGGTTT TCCTATTGGA CGCTCATCTC CTAGAGGTTC TGATTTTGGG 
AGTGACATCT CATATAACAG CCCAATACTA CATGTGTATG AAAAGGATTT CTCATCTGAG 
GTCTACTGTG GTTCTTTGTG GGGAGTCAAT TTGTTGCTGG GCACCCAATC TCATCTGTAT 
CTGATGGACC GAAGTGGAAA GGCAGAAATT GTTAAACTTA TAAAGCGAAG ACCATTCCGG 
CAGATTCAAG TCGTGGAGCA GCTCAATTTG CTGATTACCA TCTCTGGCAA AAAGAACAGA 
CTTCGCGTGT ATCATCTGTC TTGGCTGAGG AACAAAATTC TGAATAATGA TCCAAAGAGT 
AAGAAAAGGC AGAAGGCGAT GAGGAAGAAA GAGGAAGCTT GCAAAGCTAT CGATAAGTTG 
ATTGGCTGTG AACACTTCAG TGTCCTTCAA CATGAAGAAA CAACATACAT TGCGGTTGCT 
GTGAAGTCAT CAATTCACCT CTTTGCATGG GCACCAAAAT CCTTTGATGA AAACACTGCC 
ATTAAAGTAT TATGCATTGA CCAATCAGCA GACTCCGAAG GAGACTACAT GTCCTATGAA 
GCCTATATAC GAATATTGGC AAAAATACAG GCAGCTGATC CAGCGAACCG GTTTAAGAGA 
CCAGATGAGC TCCTTCATTT GCTGAAGCTC AAGGTGTTTC CAACTCGTGA TCTTAAGCCA 
CTGACAGTTG ACCTGGCTGT TGGTTCAGAG AAAACACTGA AGATTTTCTT CAGCTCAGCT 
AATGGATATC ATATCATCGA TGCAGAATCT GAAGTTATGT CTGAGGTGAC CTTGCCAAAT 
AATTCACTGG AAATCATTAT CCCACAGAAT GTCGTCATTT TACCTGATTG CTTGGGACTT 
GGTGTGATGC TCTCCCTCAA TGCTGAAGCT GCCTCCGAGG AAGCAAACGA ACAACTCCTT 
AAGAAGATCC TGGACGTGTG GAAGGACATA CCGTCTTCTG TGGCTTTTGA GTGTACAAAG 
AGAATCACTG GATGGGACCA AAAGGCTATT GAAGTGCGCT CTCTTCAGTC AACTATTCTC 
GAGAATGAGC TGAAGCGCCG CTCAATCAAG AAACTGAGAT TTCTCTGTGC CCGTGGTGAC 
AAGATGTTCT TTGCTTCTAC ACTGAGCAAT GACCACAGCC GGGTTTACCT TATGAGCCTT 
GGAAAACTTG AAGAGCTCCA TAGAAGCTAT GCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528613.1
CDS	435..4973
Translation	MAGPGSWRDKEVTDLGQLPDPTGIFSLDKAIGLGTYGRIFLGIHEKTGSLVAVKVMSARKTPLPEIGRRVRVNKYQKSVGWRYSDEEEDLRTELNLLRKYSFHKNIVTFYGAFFKLNPPGHQHQLWMVMELCAAGSVTDVVRMTRNQSLKEDWIAYICREILQGLAHLHAHQVIHRDIKGQNVLLTHDAEVKIVDFGVSAQVSRTNGRRNSFIGTPYWMAPEVIHCDEDPRCSYDYRSDVWSVGITAIEMAEGAPPLCKLQPLEALCVILREAAPKVKSSGWSRKFQNFMENCMIKNFLFRPTSGNMLLHPFVHDIKNERRVVESLTKHLTGIIQKREKKGIPVAFEGEEAAKEQYITRRFRGPSCTPELLRVPTSSRCRPLRVLHGEPPQPRWLPDQEDPQDQELQQLQKAAGVFMPLHSQDNTSKLFPKQVEVAPYLRGAAQVVMPVLVQVEAPPQVSKAAQMLKSLPTQDNKATSPEVQAPVAEGQQAQHEALETEQPKDLDQVPEEFQGQDRAPEQPRQGQAAEQQQIHNPVPEQPPEEDREPEQAEVQEEAVEPPQAEIEDKEPEVVQVHAQVLLPLLSQNRHVLLPLHLDRQLLIPVGEQNEEVPRAQAWDLEASRAVGAVQALIEGLSRDLLRAPNAFVTKPLGPLQIFLENLSTDGFYTEPEPTQKKKSKVASLRKAIAKRLRPKRFRAKALWRLEDFEFSDVETSRRRRHRRWEDIFNQHEEQLRRVENDREDDSSDNDEVFHSIQAEVQIEPHAANPAGNEVHERSAPMPCNRNRTHRVKFSPSVGEEEPSLEEAQPQQQQQQPMNIRPRNCLNPQNFQAQSDSSSEEDSPVTRRKSQSSPPYSTIDQKLLIDIHVPDGFKVGKISPPVYLTNEWVGYNALSEIFWDDWIMPTRPARPPEEDGDYVELYDADANANGDEEVANGAYEDPRDGANGHDDMNNQLDQANGYEGHGAAGYNGGDVGGNHGAAFNGPRANYPRAGILKNGHNDGRALNRGAFGVFGDNAARAFHGAAGEAGAAFGNHGANRGNGRGNRNREANGRNEENGAFGRDQHVFPEFEHEESDRGTETSDSIALEITSFDGEQNSGRPVSSTTMGFPIGRSSPRGSDFGSDISYNSPILHVYEKDFSSEVYCGSLWGVNLLLGTQSHLYLMDRSGKAEIVKLIKRRPFRQIQVVEQLNLLITISGKKNRLRVYHLSWLRNKILNNDPKSKKRQKAMRKKEEACKAIDKLIGCEHFSVLQHEETTYIAVAVKSSIHLFAWAPKSFDENTAIKVLCIDQSADSEGDYMSYEAYIRILAKIQAADPANRFKRPDELLHLLKLKVFPTRDLKPLTVDLAVGSEKTLKIFFSSANGYHIIDAESEVMSEVTLPNNSLEIIIPQNVVILPDCLGLGVMLSLNAEAASEEANEQLLKKILDVWKDIPSSVAFECTKRITGWDQKAIEVRSLQSTILENELKRRSIKKLRFLCARGDKMFFASTLSNDHSRVYLMSLGKLEELHRSYAV

Target ORF information:

RefSeq Version	XM_006528550.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nik related kinase (Nrk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528550.3

ORF Insert Sequence:

ATGGCAGGAC CTGGGAGTTG GAGGGACAAG GAGGTGACAG ATCTTGGCCA GTTGCCGGAT 
CCAACTGGAA TATTCTCACT AGATAAAGCC ATTGGTCTTG GTACTTATGG AAGAATCTTT 
TTGGGAATCC ATGAAAAGAC TGGTTCATTG GTGGCTGTTA AAGTAATGAG TGCCAGGAAG 
ACTCCTTTGC CTGAGATAGG AAGACGGGTG AGAGTGAATA AATACCAAAA GTCTGTTGGA 
TGGAGATACA GCGATGAAGA AGAGGATCTT AGGACGGAAC TCAACCTGCT GAGGAAGTAC 
TCTTTCCACA AAAACATTGT GACCTTCTAT GGAGCATTTT TCAAGCTGAA TCCTCCTGGC 
CATCAGCACC AACTTTGGAT GGTAATGGAG CTATGTGCAG CAGGCTCAGT CACTGATGTA 
GTGAGGATGA CAAGAAATCA GAGTTTAAAA GAAGACTGGA TCGCTTATAT CTGTCGAGAA 
ATCCTTCAGG GCTTAGCTCA CCTTCATGCA CACCAAGTAA TTCACCGGGA CATCAAAGGA 
CAAAATGTGC TGCTGACTCA TGATGCTGAA GTCAAAATAG TGGATTTTGG AGTGAGTGCC 
CAAGTGAGCA GAACAAATGG ACGGAGAAAT AGCTTCATTG GTACACCGTA CTGGATGGCC 
CCAGAGGTGA TTCATTGTGA TGAGGACCCA AGGTGTTCCT ATGATTACAG AAGTGATGTG 
TGGTCTGTGG GAATCACTGC TATTGAAATG GCTGAAGGGG CTCCTCCTCT GTGTAAGCTT 
CAGCCTCTGG AAGCCCTTTG TGTTATTCTA CGGGAAGCCG CTCCCAAAGT CAAATCCAGT 
GGATGGTCCC GTAAGTTCCA GAATTTCATG GAAAATTGCA TGATAAAAAA TTTCCTATTT 
CGTCCTACAT CTGGAAACAT GCTTCTTCAC CCATTTGTCC ATGATATAAA AAATGAACGA 
CGTGTTGTGG AGTCCTTGAC AAAACATCTT ACTGGGATCA TTCAAAAGAG AGAGAAAAAA 
GGAATACCTG TGGCCTTTGA AGGAGAAGAA GCTGCTAAGG AACAGTACAT CACAAGGAGA 
TTCAGAGGGC CTTCTTGCAC TCCTGAGCTT CTCAGAGTGC CAACCAGCAG CAGATGCAGA 
CCACTCAGAG TCCTGCATGG AGAGCCCCCT CAGCCAAGGT GGCTACCTGA TCAAGAAGAC 
CCACAGGACC AAGAACTTCA ACAACTGCAG AAGGCAGCCG GAGTGTTCAT GCCTCTACAT 
TCTCAGGACA ATACATCCAA GCTTTTCCCA AAGCAGGTTG AGGTAGCACC CTATCTCCGT 
GGGGCAGCTC AGGTGGTCAT GCCAGTACTG GTACAGGTTG AAGCACCGCC CCAAGTAAGC 
AAAGCAGCCC AGATGCTCAA GTCACTCCCC ACTCAGGACA ACAAAGCTAC TTCTCCAGAG 
GTCCAGGCCC CCGTAGCCGA AGGACAGCAG GCCCAGCATG AGGCCTTAGA GACAGAACAG 
CCTAAAGATT TGGACCAGGT GCCAGAAGAA TTTCAGGGGC AAGACAGGGC ACCTGAACAG 
CCAAGGCAGG GCCAGGCTGC TGAGCAGCAG CAGATACATA ATCCGGTTCC TGAGCAGCCG 
CCGGAGGAGG ATAGGGAGCC TGAGCAAGCT GAGGTGCAGG AAGAGGCTGT GGAGCCTCCC 
CAAGCTGAGA TCGAGGACAA GGAACCTGAG GTAGTGCAAG TGCACGCCCA GGTGCTCCTG 
CCTTTACTGT CCCAGAACCG TCACGTGCTC TTGCCTCTTC ATTTGGATAG ACAGCTGCTC 
ATTCCGGTAG GGGAACAAAA TGAAGAGGTA CCACGAGCCC AGGCCTGGGA TCTAGAGGCC 
TCAAGGGCAG TTGGTGCAGT GCAAGCGCTG ATCGAGGGAC TATCCAGGGA CTTGCTTCGA 
GCACCAAATG CATTTGTCAC AAAACCACTT GGTCCGCTGC AGATCTTCTT GGAAAATTTG 
TCTACAGACG GGTTTTACAC TGAGCCGGAA CCGACACAGA AGAAAAAGTC GAAAGTTGCT 
AGTCTAAGGA AAGCAATAGC AAAACGACTA AGACCCAAGA GGTTCAGAGC AAAGGCATTA 
TGGCGACTTG AAGATTTTGA ATTCTCAGAT GTAGAAACCA GCAGGAGACG GCGCCACCGT 
AGATGGGAGG ACATCTTTAA TCAGCATGAA GAGCAACTGA GACGAGTTGA AAATGACAGA 
GAAGATGACT CATCAGACAA TGATGAAGTG TTTCATTCAA TTCAGGCTGA AGTCCAAATA 
GAACCACACG CTGCAAATCC TGCAGGAAAT GAGGTCCATG AGAGATCTGC TCCCATGCCT 
TGCAACCGGA ATCGCACACA TCGTGTGAAA TTTTCTCCAA GTGTTGGTGA GGAAGAGCCT 
TCTTTGGAAG AAGCCCAGCC GCAGCAGCAG CAGCAGCAGC CCATGAACAT CCGACCGAGG 
AATTGCCTAA ATCCTCAGAA TTTTCAAGCT CAATCAGACT CATCTTCTGA GGAGGACAGT 
CCTGTGACTA GGAGGAAGTC CCAGTCATCA CCGCCTTATT CTACTATTGA TCAGAAGCTA 
CTGATTGATA TCCACGTTCC AGATGGATTT AAAGTGGGGA AAATATCACC TCCTGTGTAC 
TTGACAAATG AATGGGTAGG CTATAATGCA CTCTCTGAAA TCTTCTGGGA TGACTGGATA 
ATGCCTACAC GTCCCGCTCG GCCACCTGAA GAAGATGGTG ATTATGTTGA ACTCTATGAT 
GCTGATGCCA ATGCTAATGG TGATGAAGAA GTTGCAAACG GTGCTTATGA AGATCCTCGT 
GATGGTGCCA ATGGCCATGA TGACATGAAT AACCAGCTTG ATCAAGCAAA TGGTTATGAA 
GGCCATGGTG CTGCTGGCTA CAATGGTGGA GACGTTGGTG GTAATCATGG TGCTGCTTTC 
AATGGGCCAA GAGCAAATTA TCCCAGAGCG GGAATCTTGA AAAACGGTCA TAATGATGGA 
CGTGCTCTAA ACAGGGGAGC TTTTGGAGTC TTTGGAGACA ATGCAGCCAG AGCTTTCCAT 
GGAGCTGCCG GTGAAGCAGG TGCAGCCTTT GGAAACCATG GAGCCAATAG GGGCAATGGA 
AGAGGTAACA GAAACAGGGA GGCCAATGGA AGGAATGAAG AGAATGGAGC ATTTGGACGG 
GATCAACATG TATTTCCAGA GTTTGAACAT GAGGAATCAG ATCGTGGAAC TGAGACCTCA 
GATTCAATTG CCTTGGAGAT CACATCCTTT GATGGTGAGC AAAACAGTGG TAGACCAGTA 
TCATCAACAA CAATGGGTTT TCCTATTGGA CGCTCATCTC CTAGAGGTTC TGATTTTGGG 
AGTGACATCT CATATAACAG CCCAATACTA CATGTGTATG AAAAGGATTT CTCATCTGAG 
GTCTACTGTG GTTCTTTGTG GGGAGTCAAT TTGTTGCTGG GCACCCAATC TCATCTGTAT 
CTGATGGACC GAAGTGGAAA GGCAGAAATT GTTAAACTTA TAAAGCGAAG ACCATTCCGG 
CAGATTCAAG TCGTGGAGCA GCTCAATTTG CTGATTACCA TCTCTGGCAA AAAGAACAGA 
CTTCGCGTGT ATCATCTGTC TTGGCTGAGG AACAAAATTC TGAATAATGA TCCAAAGAGT 
AAGAAAAGGC AGAAGGCGAT GAGGAAGAAA GAGGAAGCTT GCAAAGCTAT CGATAAGTTG 
ATTGGCTGTG AACACTTCAG TGTCCTTCAA CATGAAGAAA CAACATACAT TGCGGTTGCT 
GTGAAGTCAT CAATTCACCT CTTTGCATGG GCACCAAAAT CCTTTGATGA AAACACTGCC 
ATTAAAGTAT TATGCATTGA CCAATCAGCA GACTCCGAAG GAGACTACAT GTCCTATGAA 
GCCTATATAC GAATATTGGC AAAAATACAG GCAGCTGATC CAGCGAACCG GTTTAAGAGA 
CCAGATGAGC TCCTTCATTT GCTGAAGCTC AAGGTGTTTC CAACTCGTGA TCTTAAGCCA 
CTGACAGTTG ACCTGGCTGT TGGTTCAGAG AAAACACTGA AGATTTTCTT CAGCTCAGCT 
AATGGATATC ATATCATCGA TGCAGAATCT GAAGTTATGT CTGAGGTGAC CTTGCCAAAT 
AATTCACTGG AAATCATTAT CCCACAGAAT GTCGTCATTT TACCTGATTG CTTGGGACTT 
GGTGTGATGC TCTCCCTCAA TGCTGAAGCT GCCTCCGAGG AAGCAAACGA ACAACTCCTT 
AAGAAGATCC TGGACGTGTG GAAGGACATA CCGTCTTCTG TGGCTTTTGA GTGTACAAAG 
AGAATCACTG GATGGGACCA AAAGGCTATT GAAGTGCGCT CTCTTCAGTC AACTATTCTC 
GAGAATGAGC TGAAGCGCCG CTCAATCAAG AAACTGAGAT TTCTCTGTGC CCGTGGTGAC 
AAGATGTTCT TTGCTTCTAC ACTGAGCAAT GACCACAGCC GGGTTTACCT TATGAGCCTT 
GGAAAACTTG AAGAGCTCCA TAGAAGCTAT GCTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nik-related kinase regulates trophoblast proliferation and placental development by modulating AKT phosphorylation.
PloS one12(2)e0171503(2017)
Morioka Y,Nam JM,Ohashi T

Deficiency of X-Linked Protein Kinase Nrk during Pregnancy Triggers Breast Tumor in Mice.
The American journal of pathology186(10)2751-60(2016 Oct)
Yanagawa T,Denda K,Inatani T,Fukushima T,Tanaka T,Kumaki N,Inagaki Y,Komada M

Nrk, an X-linked protein kinase in the germinal center kinase family, is required for placental development and fetoplacental induction of labor.
The Journal of biological chemistry286(33)28802-10(2011 Aug)
Denda K,Nakao-Wakabayashi K,Okamoto N,Kitamura N,Ryu JY,Tagawa Y,Ichisaka T,Yamanaka S,Komada M

Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family.
Experimental cell research287(2)219-27(2003 Jul)
Nakano K,Kanai-Azuma M,Kanai Y,Moriyama K,Yazaki K,Hayashi Y,Kitamura N

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Nrk cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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