Degs2 cDNA ORF clone, Mus musculus(house mouse)
| Gene Symbol | Degs2 |
| Entrez Gene ID | 70059 |
| Full Name | delta(4)-desaturase, sphingolipid 2 |
| Synonyms | 2210008A03Rik,AI852933,DES2 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_001171002.1 | NP_001164473.1 | sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 2 |
| NM_027299.5 | NP_081575.2 | sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 1 |
| Danio rerio (zebrafish) | degs2 | NP_001156838.1 |
| Caenorhabditis elegans (roundworm) | F33D4.4 | NP_501256.1 |
| Magnaporthe oryzae (rice blast fungus) | MGG_13187 | XP_003712066.1 |
| Pan troglodytes (chimpanzee) | DEGS2 | XP_001157834.1 |
| LOC717222 | XP_001108524.1 | |
| Canis lupus familiaris (dog) | DEGS2 | XP_547976.2 |
| Mus musculus (house mouse) | Degs2 | NP_081575.2 |
| Kluyveromyces lactis | KLLA0F18656g | XP_455916.1 |
| Bos taurus (cattle) | DEGS2 | NP_001069719.1 |
| Drosophila melanogaster (fruit fly) | ifc | NP_476594.1 |
| Caenorhabditis elegans (roundworm) | ttm-5 | NP_493549.1 |
| Oryza sativa (rice) | Os02g0639600 | NP_001047540.1 |
| Xenopus tropicalis (tropical clawed frog) | degs2 | XP_002936627.1 |
| Homo sapiens (human) | DEGS2 | NP_996801.2 |
| Rattus norvegicus (Norway rat) | Degs2 | NP_001017457.1 |
| Gallus gallus (chicken) | DEGS2 | XP_421364.2 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP011402 | XP_317914.3 |
| Eremothecium gossypii | AGOS_AGR025W | NP_986690.2 |
| Schizosaccharomyces pombe (fission yeast) | dsd1 | NP_596407.1 |
| Neurospora crassa | NCU08927 | XP_960609.1 |
| Arabidopsis thaliana (thale cress) | DES-1-LIKE | NP_192402.1 |
Related articles in PubMed
Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil.
Lau CG, Marikawa Y
Molecular reproduction and development81(11)994-1008(2014 Nov)
Dihydroceramide-based response to hypoxia.
Devlin CM, Lahm T, Hubbard WC, Van Demark M, Wang KC, Wu X, Bielawska A, Obeid LM, Ivan M, Petrache I
The Journal of biological chemistry286(44)38069-78(2011 Nov)
Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.
Enomoto A, Omae F, Miyazaki M, Kozutsumi Y, Yubisui T, Suzuki A
The Biochemical journal397(2)289-95(2006 Jul)
Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.
Omae F, Miyazaki M, Enomoto A, Suzuki A
FEBS letters576(1-2)63-7(2004 Oct)
DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine.
Omae F, Miyazaki M, Enomoto A, Suzuki M, Suzuki Y, Suzuki A
The Biochemical journal379(Pt 3)687-95(2004 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
overexpression of DEGS1 or DEGS2 attenuates the DHC accumulation and increased cell proliferation during hypoxia
Title: Dihydroceramide-based response to hypoxia.
the hydroxylation of dihydroceramide: sphinganine C-4 requires complex formation between Des2 and mb5 via their membrane-spanning domains and electron transfer from NADH to the substrate via the reduction of mb5 by b5R
Title: Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.
DES2 is the dihydroceramide C-4 hydroxylase responsible for the biosynthesis of enriched phytosphingoglycolipids in the microvillous membranes of intestinal epithelial cells
Title: DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine.
The following Degs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Degs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu11778 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001171002.1 | |
| Accession Version | NM_001171002.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-02 | |
| Organism | Mus musculus(house mouse) | |
| Product | sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008666.1, AK162979.1 and AC139568.5. Transcript Variant: This variant (2) has an alternate 3' exon, as compared to variant 1. The resulting isoform (2) is shorter and has a different C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK162979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001164473.1 |
| CDS | 65..925 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001171002.1 |
ORF Insert Sequence:
1 | ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu04010 | |
| Clone ID Related Accession (Same CDS sequence) | NM_027299.5 | |
| Accession Version | NM_027299.5 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-02 | |
| Organism | Mus musculus(house mouse) | |
| Product | sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008666.1, AK162979.1 and BU757432.1. On Jan 8, 2010 this sequence version replaced NM_027299.4. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC016427.1, AF466377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_081575.2 |
| CDS | 65..1036 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_027299.5 |
ORF Insert Sequence:
1 | ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil. |
|
Dihydroceramide-based response to hypoxia. |
|
Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5. |
|
Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2. |
|
DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine. |