Ttc21b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ttc21b
Entrez Gene ID	73668
Full Name	tetratricopeptide repeat domain 21B
Synonyms	2410066K11Rik,Thm1,aln,mKIAA1992
General protein information	Preferred Names tetratricopeptide repeat domain 21B Names tetratricopeptide repeat protein 21B TPR repeat protein 21B intraflagellar transport 139 homolog line 158 tetratricopeptide repeat-containing hedgehog modulator 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 C1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006500263.3	XP_006500326.1	tetratricopeptide repeat protein 21B isoform X1
XM_030252122.1	XP_030107982.1	tetratricopeptide repeat protein 21B isoform X2
NM_001290669.1	NP_001277598.1	tetratricopeptide repeat protein 21B isoform b
XM_006500263.4	XP_006500326.1	tetratricopeptide repeat protein 21B isoform X1
NM_001047604.2	NP_001041069.1	tetratricopeptide repeat protein 21B isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TTC21B	XP_850862.2
Homo sapiens (human)	TTC21B	NP_079029.3
Bos taurus (cattle)	TTC21B	NP_001095578.1
Rattus norvegicus (Norway rat)	Ttc21b	NP_001178666.1
Gallus gallus (chicken)	TTC21B	XP_422022.3
Pan troglodytes (chimpanzee)	TTC21B	XP_003309329.1
Mus musculus (house mouse)	Ttc21b	NP_001041069.1
Danio rerio (zebrafish)	ttc21b	NP_001121730.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000136	XP_311010.5
	TTC21B	XP_001096248.2
Caenorhabditis elegans (roundworm)	ZK328.7	NP_001022993.2
Xenopus tropicalis (tropical clawed frog)	ttc21b	XP_002936805.2

Gene Ontology
Bibliography

Related articles in PubMed

A tissue-specific role for intraflagellar transport genes during craniofacial development.
Schock EN, Struve JN, Chang CF, Williams TJ, Snedeker J, Attia AC, Stottmann RW, Brugmann SA
PloS one12(3)e0174206(2017)

Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development.
Snedeker J, Schock EN, Struve JN, Chang CF, Cionni M, Tran PV, Brugmann SA, Stottmann RW
PloS one12(3)e0173258(2017)

Dysfunction of intraflagellar transport-A causes hyperphagia-induced obesity and metabolic syndrome.
Jacobs DT, Silva LM, Allard BA, Schonfeld MP, Chatterjee A, Talbott GC, Beier DR, Tran PV
Disease models & mechanisms9(7)789-98(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

genetically ablated Kif3a, Ift88, and Ttc21b in a series of specific spatiotemporal domains. The resulting phenotypes allow us to draw several conclusions. First, we conclude that the Ttc21b cortical phenotype is not due to the activity of Ttc21b within the brain itself
Title: Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development.

Thm1-mutant ciliary defect diminishes sensitivity to feeding signals, which alters appetite regulation and leads to hyperphagia, obesity and metabolic disease.
Title: Dysfunction of intraflagellar transport-A causes hyperphagia-induced obesity and metabolic syndrome.

loss of Thm1 causes cystic kidney disease, with persistent proliferation of renal cells, elevated cAMP levels, and enhanced expression of Hedgehog signaling genes
Title: Downregulating hedgehog signaling reduces renal cystogenic potential of mouse models.

Data characterize the forebrain phenotype caused by loss of Ttc21b, a gene we identified in an ENU mutagenesis screen as a novel ciliary gene required for retrograde intraflagellar transport.
Title: Ttc21b is required to restrict sonic hedgehog activity in the developing mouse forebrain.

The following Ttc21b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ttc21b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30943	XM_006500263.4 Latest version!	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu88024	XM_030252122.1 Latest version!	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13939	NM_001047604.2 Latest version!	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$776.30 $1109.00
OMu17401	NM_001290669.1 Latest version!	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu30943	XM_006500263.3	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ttc21b ( XM_006500263.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500326.1 Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30943
Clone ID Related Accession (Same CDS sequence)	XM_006500263.3 , XM_006500263.4
Accession Version	XM_006500263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tetratricopeptide repeat protein 21B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500263.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGGATC ATATCCCAGC TCTCTTGGGA 
ATGGCAACGG CTTATATGAT CTTGAAACAG ACTCCAAAAG CCAGAAACCA GCTGAAGCGG 
ATTGCAAAAA TGCCTTGGAA TCCTATTGAA GCCGAGGATC TGGAGAAGAG CTGGCTGCTG 
CTTGCTGATA TTTATATCCA GTCAGCAAAA TATGACATGG CAGAGGAATT ACTAAAGCGG 
TGTCTCTGCC ATAACCGGTC CTGCTGCAAA GCTTATGAAT ATATGGGGTA CATTATGGAG 
AAAGAGCAAG CATACACAGA TGCGGCCTTC AACTATGAGA TGGCATGGAA ACATAGCAAC 
CAGACAAATC CTGCAGTGGG ATACAAATTG GCATTTAATT ACCTAAAAGC AAAACGATAT 
GTGGATGCAA TTGACGTATG TCACCAGGTT CTTGAAGCAC ATCCAACTTA CCCCAAAATC 
AGAAAGGATA TACTTGATAA GGCGCGTGCA TCTTTAAGAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500326.1
CDS	141..4085
Translation	MDSQGLKTLINYYCQERYYHHVLLVASEGMKKYSSDPVFRFYHAYGTLMEGKAQEALREFEAIKNKQDVSLCSLMALMYVHKMSPNPDREAILELDTKMKEQRKEAGRKALYHAGLFLWHIGRHDKAREYIDRMSKMPHDSNEGPILKAWLDITRGKEPYAKKALRYFEEGLQDGNDIFALLGKVLCLEIRQNYSGALETVSQIIVNFPSFLPAFEKKMKLQLALQDWDQTVETAQRLLLQDNHNVEALRMLALYYLCREGDVEKAATKLENLGNALDVMEPQNAQLFYKITLAFSRTCGRNQLILQKVQSFLEKAFSLTPQQAEIATELGYQMILQGKVKEAWKWYRTAMTLNESNISAVTGLIRCQLIEGQLQDADQQLEFFSEFQQSMGKSAELMYLHAVLATKKNNRQDEVINLLNDVVNTHFSHLEDLPLGIQYFEKLNPDFLLEVVTEYLNLCPIQPAGPGQPLSPVLRRCSSVLETIIRSVPGLPQAVFLMAKVKYLSGDTEAAYNNLQHCLEHSPSYAEAHLLMAQVYLSQDKVKLCSQSLELCLSYNFNVREYPLYHLIKAQSQKKMGEVAEAIKTLHMAMNLPGMRRSRASSKSKHRTEVDASHRLSIFLELVEVHRLNGEQHEAAKVLQDAIHEFSGTCEELRVTIANADLALAQGDTDRALSMLRNVTTEQPYFIEAKEKMADIYLKHRKEKMLYITCYREIAERMPSPRSFLLLGDAYMNIQEPEEAIVAYEQALNQNPKDGTLARKIGKALVKTHNYSKAITYYEAALKSGQQNCLCYDLAELLLRLKLYEKAEKVLQHSLAHEPVSELSALMVDGRSQVLLAKVYSKMERPSDAIAALQQARELQARILKRVQMEQPDAVPSQKHFAAEICAEIAKHSAAQRDYEKAITFYREALVHCETDSKIMLELAQLYLAQEDLDASLRHCALLLQRDQDNEPATMLMADLMFRKQDYEQAVYHLQQLLDRKPDNFMTLSRLIDLLRRCGKLEDVPRFFLMAEKHNSRTKLEPGFQYCKGLHFWYTGEPNDALRHFNKARKDSDWGQNALYNMIEICLNPDNETIGGEVFENLNGDLGTSPEKQESVQLAVRTAEKLLKELKPQTVQGRLQLRIMENCCLMATKQKSSVEQALNTFTEIAASEDHIPALLGMATAYMILKQTPKARNQLKRIAKMPWNPIEAEDLEKSWLLLADIYIQSAKYDMAEELLKRCLCHNRSCCKAYEYMGYIMEKEQAYTDAAFNYEMAWKHSNQTNPAVGYKLAFNYLKAKRYVDAIDVCHQVLEAHPTYPKIRKDILDKARASLRP

Target ORF information:

RefSeq Version	XM_006500263.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500263.4

ORF Insert Sequence:

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGGATC ATATCCCAGC TCTCTTGGGA 
ATGGCAACGG CTTATATGAT CTTGAAACAG ACTCCAAAAG CCAGAAACCA GCTGAAGCGG 
ATTGCAAAAA TGCCTTGGAA TCCTATTGAA GCCGAGGATC TGGAGAAGAG CTGGCTGCTG 
CTTGCTGATA TTTATATCCA GTCAGCAAAA TATGACATGG CAGAGGAATT ACTAAAGCGG 
TGTCTCTGCC ATAACCGGTC CTGCTGCAAA GCTTATGAAT ATATGGGGTA CATTATGGAG 
AAAGAGCAAG CATACACAGA TGCGGCCTTC AACTATGAGA TGGCATGGAA ACATAGCAAC 
CAGACAAATC CTGCAGTGGG ATACAAATTG GCATTTAATT ACCTAAAAGC AAAACGATAT 
GTGGATGCAA TTGACGTATG TCACCAGGTT CTTGAAGCAC ATCCAACTTA CCCCAAAATC 
AGAAAGGATA TACTTGATAA GGCGCGTGCA TCTTTAAGAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttc21b ( XM_030252122.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107982.1 Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu88024
Clone ID Related Accession (Same CDS sequence)	XM_030252122.1
Accession Version	XM_030252122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tetratricopeptide repeat protein 21B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGGATC ATATCCCAGC TCTCTTGGGA 
ATGGCAACGG CTTATATGAT CTTGAAACAG ACTCCAAAAG CCAGAAACCA GCTGAAGCGG 
ATTGCAAAAA TGCCTTGGAA TCCTATTGAA GCCGAGGATC TGGAGAAGAG CTGGCTGCTG 
CTTGCTGATA TTTATATCCA GTCAGCAAAA TATGACATGG CAGAGGAATT ACTAAAGCGG 
TGTCTCTGCC ATAACCGGTC CTGCTGCAAA GCTTATGAAT ATATGGGGTA CATTATGGAG 
AAAGAGCAAG CATACACAGA TGCGGCCTTC AACTATGAGA TGGCATGGAA ACATAGCAAC 
CAGACAAATC CTGCAGTGGG TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107982.1
CDS	141..3947
Translation	MDSQGLKTLINYYCQERYYHHVLLVASEGMKKYSSDPVFRFYHAYGTLMEGKAQEALREFEAIKNKQDVSLCSLMALMYVHKMSPNPDREAILELDTKMKEQRKEAGRKALYHAGLFLWHIGRHDKAREYIDRMSKMPHDSNEGPILKAWLDITRGKEPYAKKALRYFEEGLQDGNDIFALLGKVLCLEIRQNYSGALETVSQIIVNFPSFLPAFEKKMKLQLALQDWDQTVETAQRLLLQDNHNVEALRMLALYYLCREGDVEKAATKLENLGNALDVMEPQNAQLFYKITLAFSRTCGRNQLILQKVQSFLEKAFSLTPQQAEIATELGYQMILQGKVKEAWKWYRTAMTLNESNISAVTGLIRCQLIEGQLQDADQQLEFFSEFQQSMGKSAELMYLHAVLATKKNNRQDEVINLLNDVVNTHFSHLEDLPLGIQYFEKLNPDFLLEVVTEYLNLCPIQPAGPGQPLSPVLRRCSSVLETIIRSVPGLPQAVFLMAKVKYLSGDTEAAYNNLQHCLEHSPSYAEAHLLMAQVYLSQDKVKLCSQSLELCLSYNFNVREYPLYHLIKAQSQKKMGEVAEAIKTLHMAMNLPGMRRSRASSKSKHRTEVDASHRLSIFLELVEVHRLNGEQHEAAKVLQDAIHEFSGTCEELRVTIANADLALAQGDTDRALSMLRNVTTEQPYFIEAKEKMADIYLKHRKEKMLYITCYREIAERMPSPRSFLLLGDAYMNIQEPEEAIVAYEQALNQNPKDGTLARKIGKALVKTHNYSKAITYYEAALKSGQQNCLCYDLAELLLRLKLYEKAEKVLQHSLAHEPVSELSALMVDGRSQVLLAKVYSKMERPSDAIAALQQARELQARILKRVQMEQPDAVPSQKHFAAEICAEIAKHSAAQRDYEKAITFYREALVHCETDSKIMLELAQLYLAQEDLDASLRHCALLLQRDQDNEPATMLMADLMFRKQDYEQAVYHLQQLLDRKPDNFMTLSRLIDLLRRCGKLEDVPRFFLMAEKHNSRTKLEPGFQYCKGLHFWYTGEPNDALRHFNKARKDSDWGQNALYNMIEICLNPDNETIGGEVFENLNGDLGTSPEKQESVQLAVRTAEKLLKELKPQTVQGRLQLRIMENCCLMATKQKSSVEQALNTFTEIAASEDHIPALLGMATAYMILKQTPKARNQLKRIAKMPWNPIEAEDLEKSWLLLADIYIQSAKYDMAEELLKRCLCHNRSCCKAYEYMGYIMEKEQAYTDAAFNYEMAWKHSNQTNPAVGS

Target ORF information:

RefSeq Version	XM_030252122.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252122.1

ORF Insert Sequence:

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGGATC ATATCCCAGC TCTCTTGGGA 
ATGGCAACGG CTTATATGAT CTTGAAACAG ACTCCAAAAG CCAGAAACCA GCTGAAGCGG 
ATTGCAAAAA TGCCTTGGAA TCCTATTGAA GCCGAGGATC TGGAGAAGAG CTGGCTGCTG 
CTTGCTGATA TTTATATCCA GTCAGCAAAA TATGACATGG CAGAGGAATT ACTAAAGCGG 
TGTCTCTGCC ATAACCGGTC CTGCTGCAAA GCTTATGAAT ATATGGGGTA CATTATGGAG 
AAAGAGCAAG CATACACAGA TGCGGCCTTC AACTATGAGA TGGCATGGAA ACATAGCAAC 
CAGACAAATC CTGCAGTGGG TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttc21b ( NM_001047604.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001041069.1 Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant 1, mRNA.

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CloneID	OMu13939
Clone ID Related Accession (Same CDS sequence)	NM_001047604.2
Accession Version	NM_001047604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	tetratricopeptide repeat protein 21B isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844526.5 and AL928586.10. On Mar 15, 2014 this sequence version replaced NM_001047604.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ011164.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGAAGG ATCATATCCC AGCTCTCTTG 
GGAATGGCAA CGGCTTATAT GATCTTGAAA CAGACTCCAA AAGCCAGAAA CCAGCTGAAG 
CGGATTGCAA AAATGCCTTG GAATCCTATT GAAGCCGAGG ATCTGGAGAA GAGCTGGCTG 
CTGCTTGCTG ATATTTATAT CCAGTCAGCA AAATATGACA TGGCAGAGGA ATTACTAAAG 
CGGTGTCTCT GCCATAACCG GTCCTGCTGC AAAGCTTATG AATATATGGG GTACATTATG 
GAGAAAGAGC AAGCATACAC AGATGCGGCC TTCAACTATG AGATGGCATG GAAACATAGC 
AACCAGACAA ATCCTGCAGT GGGATACAAA TTGGCATTTA ATTACCTAAA AGCAAAACGA 
TATGTGGATG CAATTGACGT ATGTCACCAG GTTCTTGAAG CACATCCAAC TTACCCCAAA 
ATCAGAAAGG ATATACTTGA TAAGGCGCGT GCATCTTTAA GACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041069.1
CDS	84..4031
Translation	MDSQGLKTLINYYCQERYYHHVLLVASEGMKKYSSDPVFRFYHAYGTLMEGKAQEALREFEAIKNKQDVSLCSLMALMYVHKMSPNPDREAILELDTKMKEQRKEAGRKALYHAGLFLWHIGRHDKAREYIDRMSKMPHDSNEGPILKAWLDITRGKEPYAKKALRYFEEGLQDGNDIFALLGKVLCLEIRQNYSGALETVSQIIVNFPSFLPAFEKKMKLQLALQDWDQTVETAQRLLLQDNHNVEALRMLALYYLCREGDVEKAATKLENLGNALDVMEPQNAQLFYKITLAFSRTCGRNQLILQKVQSFLEKAFSLTPQQAEIATELGYQMILQGKVKEAWKWYRTAMTLNESNISAVTGLIRCQLIEGQLQDADQQLEFFSEFQQSMGKSAELMYLHAVLATKKNNRQDEVINLLNDVVNTHFSHLEDLPLGIQYFEKLNPDFLLEVVTEYLNLCPIQPAGPGQPLSPVLRRCSSVLETIIRSVPGLPQAVFLMAKVKYLSGDTEAAYNNLQHCLEHSPSYAEAHLLMAQVYLSQDKVKLCSQSLELCLSYNFNVREYPLYHLIKAQSQKKMGEVAEAIKTLHMAMNLPGMRRSRASSKSKHRTEVDASHRLSIFLELVEVHRLNGEQHEAAKVLQDAIHEFSGTCEELRVTIANADLALAQGDTDRALSMLRNVTTEQPYFIEAKEKMADIYLKHRKEKMLYITCYREIAERMPSPRSFLLLGDAYMNIQEPEEAIVAYEQALNQNPKDGTLARKIGKALVKTHNYSKAITYYEAALKSGQQNCLCYDLAELLLRLKLYEKAEKVLQHSLAHEPVSELSALMVDGRSQVLLAKVYSKMERPSDAIAALQQARELQARILKRVQMEQPDAVPSQKHFAAEICAEIAKHSAAQRDYEKAITFYREALVHCETDSKIMLELAQLYLAQEDLDASLRHCALLLQRDQDNEPATMLMADLMFRKQDYEQAVYHLQQLLDRKPDNFMTLSRLIDLLRRCGKLEDVPRFFLMAEKHNSRTKLEPGFQYCKGLHFWYTGEPNDALRHFNKARKDSDWGQNALYNMIEICLNPDNETIGGEVFENLNGDLGTSPEKQESVQLAVRTAEKLLKELKPQTVQGRLQLRIMENCCLMATKQKSSVEQALNTFTEIAASEKDHIPALLGMATAYMILKQTPKARNQLKRIAKMPWNPIEAEDLEKSWLLLADIYIQSAKYDMAEELLKRCLCHNRSCCKAYEYMGYIMEKEQAYTDAAFNYEMAWKHSNQTNPAVGYKLAFNYLKAKRYVDAIDVCHQVLEAHPTYPKIRKDILDKARASLRP

Target ORF information:

RefSeq Version	NM_001047604.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047604.2

ORF Insert Sequence:

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGAAGG ATCATATCCC AGCTCTCTTG 
GGAATGGCAA CGGCTTATAT GATCTTGAAA CAGACTCCAA AAGCCAGAAA CCAGCTGAAG 
CGGATTGCAA AAATGCCTTG GAATCCTATT GAAGCCGAGG ATCTGGAGAA GAGCTGGCTG 
CTGCTTGCTG ATATTTATAT CCAGTCAGCA AAATATGACA TGGCAGAGGA ATTACTAAAG 
CGGTGTCTCT GCCATAACCG GTCCTGCTGC AAAGCTTATG AATATATGGG GTACATTATG 
GAGAAAGAGC AAGCATACAC AGATGCGGCC TTCAACTATG AGATGGCATG GAAACATAGC 
AACCAGACAA ATCCTGCAGT GGGATACAAA TTGGCATTTA ATTACCTAAA AGCAAAACGA 
TATGTGGATG CAATTGACGT ATGTCACCAG GTTCTTGAAG CACATCCAAC TTACCCCAAA 
ATCAGAAAGG ATATACTTGA TAAGGCGCGT GCATCTTTAA GACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttc21b ( NM_001290669.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277598.1 Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant 2, mRNA.

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CloneID	OMu17401
Clone ID Related Accession (Same CDS sequence)	NM_001290669.1
Accession Version	NM_001290669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	tetratricopeptide repeat protein 21B isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844526.5 and AL928586.10. On Mar 15, 2014 this sequence version replaced XM_006500264.1. Transcript Variant: This variant (2) lacks an alternate exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151199.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGAAGG ATCATATCCC AGCTCTCTTG 
GGAATGGCAA CGGCTTATAT GATCTTGAAA CAGACTCCAA AAGCCAGAAA CCAGCTGAAG 
CGGATTGCAA AAATGCCTTG GAATCCTATT GAAGCCGAGG ATCTGGAGAA GAGCTGGCTG 
CTGCTTGCTG ATATTTATAT CCAGTCAGCA AAATATGACA TGGCAGAGGA ATTACTAAAG 
CGGTGTCTCT GCCATAACCG GTCCTGCTGC AAAGCTTATG AATATATGGG GTACATTATG 
GAGAAAGAGC AAGCATACAC AGATGCGGCC TTCAACTATG AGATGGCATG GAAACATAGC 
AACCAGACAA ATCCTGCAGT GGGTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277598.1
CDS	84..3893
Translation	MDSQGLKTLINYYCQERYYHHVLLVASEGMKKYSSDPVFRFYHAYGTLMEGKAQEALREFEAIKNKQDVSLCSLMALMYVHKMSPNPDREAILELDTKMKEQRKEAGRKALYHAGLFLWHIGRHDKAREYIDRMSKMPHDSNEGPILKAWLDITRGKEPYAKKALRYFEEGLQDGNDIFALLGKVLCLEIRQNYSGALETVSQIIVNFPSFLPAFEKKMKLQLALQDWDQTVETAQRLLLQDNHNVEALRMLALYYLCREGDVEKAATKLENLGNALDVMEPQNAQLFYKITLAFSRTCGRNQLILQKVQSFLEKAFSLTPQQAEIATELGYQMILQGKVKEAWKWYRTAMTLNESNISAVTGLIRCQLIEGQLQDADQQLEFFSEFQQSMGKSAELMYLHAVLATKKNNRQDEVINLLNDVVNTHFSHLEDLPLGIQYFEKLNPDFLLEVVTEYLNLCPIQPAGPGQPLSPVLRRCSSVLETIIRSVPGLPQAVFLMAKVKYLSGDTEAAYNNLQHCLEHSPSYAEAHLLMAQVYLSQDKVKLCSQSLELCLSYNFNVREYPLYHLIKAQSQKKMGEVAEAIKTLHMAMNLPGMRRSRASSKSKHRTEVDASHRLSIFLELVEVHRLNGEQHEAAKVLQDAIHEFSGTCEELRVTIANADLALAQGDTDRALSMLRNVTTEQPYFIEAKEKMADIYLKHRKEKMLYITCYREIAERMPSPRSFLLLGDAYMNIQEPEEAIVAYEQALNQNPKDGTLARKIGKALVKTHNYSKAITYYEAALKSGQQNCLCYDLAELLLRLKLYEKAEKVLQHSLAHEPVSELSALMVDGRSQVLLAKVYSKMERPSDAIAALQQARELQARILKRVQMEQPDAVPSQKHFAAEICAEIAKHSAAQRDYEKAITFYREALVHCETDSKIMLELAQLYLAQEDLDASLRHCALLLQRDQDNEPATMLMADLMFRKQDYEQAVYHLQQLLDRKPDNFMTLSRLIDLLRRCGKLEDVPRFFLMAEKHNSRTKLEPGFQYCKGLHFWYTGEPNDALRHFNKARKDSDWGQNALYNMIEICLNPDNETIGGEVFENLNGDLGTSPEKQESVQLAVRTAEKLLKELKPQTVQGRLQLRIMENCCLMATKQKSSVEQALNTFTEIAASEKDHIPALLGMATAYMILKQTPKARNQLKRIAKMPWNPIEAEDLEKSWLLLADIYIQSAKYDMAEELLKRCLCHNRSCCKAYEYMGYIMEKEQAYTDAAFNYEMAWKHSNQTNPAVGS

Target ORF information:

RefSeq Version	NM_001290669.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290669.1

ORF Insert Sequence:

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGAAGG ATCATATCCC AGCTCTCTTG 
GGAATGGCAA CGGCTTATAT GATCTTGAAA CAGACTCCAA AAGCCAGAAA CCAGCTGAAG 
CGGATTGCAA AAATGCCTTG GAATCCTATT GAAGCCGAGG ATCTGGAGAA GAGCTGGCTG 
CTGCTTGCTG ATATTTATAT CCAGTCAGCA AAATATGACA TGGCAGAGGA ATTACTAAAG 
CGGTGTCTCT GCCATAACCG GTCCTGCTGC AAAGCTTATG AATATATGGG GTACATTATG 
GAGAAAGAGC AAGCATACAC AGATGCGGCC TTCAACTATG AGATGGCATG GAAACATAGC 
AACCAGACAA ATCCTGCAGT GGGTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttc21b ( XM_006500263.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500326.1 Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30943
Clone ID Related Accession (Same CDS sequence)	XM_006500263.3 , XM_006500263.4
Accession Version	XM_006500263.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tetratricopeptide repeat protein 21B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGGATC ATATCCCAGC TCTCTTGGGA 
ATGGCAACGG CTTATATGAT CTTGAAACAG ACTCCAAAAG CCAGAAACCA GCTGAAGCGG 
ATTGCAAAAA TGCCTTGGAA TCCTATTGAA GCCGAGGATC TGGAGAAGAG CTGGCTGCTG 
CTTGCTGATA TTTATATCCA GTCAGCAAAA TATGACATGG CAGAGGAATT ACTAAAGCGG 
TGTCTCTGCC ATAACCGGTC CTGCTGCAAA GCTTATGAAT ATATGGGGTA CATTATGGAG 
AAAGAGCAAG CATACACAGA TGCGGCCTTC AACTATGAGA TGGCATGGAA ACATAGCAAC 
CAGACAAATC CTGCAGTGGG ATACAAATTG GCATTTAATT ACCTAAAAGC AAAACGATAT 
GTGGATGCAA TTGACGTATG TCACCAGGTT CTTGAAGCAC ATCCAACTTA CCCCAAAATC 
AGAAAGGATA TACTTGATAA GGCGCGTGCA TCTTTAAGAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500326.1
CDS	113..4057
Translation	MDSQGLKTLINYYCQERYYHHVLLVASEGMKKYSSDPVFRFYHAYGTLMEGKAQEALREFEAIKNKQDVSLCSLMALMYVHKMSPNPDREAILELDTKMKEQRKEAGRKALYHAGLFLWHIGRHDKAREYIDRMSKMPHDSNEGPILKAWLDITRGKEPYAKKALRYFEEGLQDGNDIFALLGKVLCLEIRQNYSGALETVSQIIVNFPSFLPAFEKKMKLQLALQDWDQTVETAQRLLLQDNHNVEALRMLALYYLCREGDVEKAATKLENLGNALDVMEPQNAQLFYKITLAFSRTCGRNQLILQKVQSFLEKAFSLTPQQAEIATELGYQMILQGKVKEAWKWYRTAMTLNESNISAVTGLIRCQLIEGQLQDADQQLEFFSEFQQSMGKSAELMYLHAVLATKKNNRQDEVINLLNDVVNTHFSHLEDLPLGIQYFEKLNPDFLLEVVTEYLNLCPIQPAGPGQPLSPVLRRCSSVLETIIRSVPGLPQAVFLMAKVKYLSGDTEAAYNNLQHCLEHSPSYAEAHLLMAQVYLSQDKVKLCSQSLELCLSYNFNVREYPLYHLIKAQSQKKMGEVAEAIKTLHMAMNLPGMRRSRASSKSKHRTEVDASHRLSIFLELVEVHRLNGEQHEAAKVLQDAIHEFSGTCEELRVTIANADLALAQGDTDRALSMLRNVTTEQPYFIEAKEKMADIYLKHRKEKMLYITCYREIAERMPSPRSFLLLGDAYMNIQEPEEAIVAYEQALNQNPKDGTLARKIGKALVKTHNYSKAITYYEAALKSGQQNCLCYDLAELLLRLKLYEKAEKVLQHSLAHEPVSELSALMVDGRSQVLLAKVYSKMERPSDAIAALQQARELQARILKRVQMEQPDAVPSQKHFAAEICAEIAKHSAAQRDYEKAITFYREALVHCETDSKIMLELAQLYLAQEDLDASLRHCALLLQRDQDNEPATMLMADLMFRKQDYEQAVYHLQQLLDRKPDNFMTLSRLIDLLRRCGKLEDVPRFFLMAEKHNSRTKLEPGFQYCKGLHFWYTGEPNDALRHFNKARKDSDWGQNALYNMIEICLNPDNETIGGEVFENLNGDLGTSPEKQESVQLAVRTAEKLLKELKPQTVQGRLQLRIMENCCLMATKQKSSVEQALNTFTEIAASEDHIPALLGMATAYMILKQTPKARNQLKRIAKMPWNPIEAEDLEKSWLLLADIYIQSAKYDMAEELLKRCLCHNRSCCKAYEYMGYIMEKEQAYTDAAFNYEMAWKHSNQTNPAVGYKLAFNYLKAKRYVDAIDVCHQVLEAHPTYPKIRKDILDKARASLRP

Target ORF information:

RefSeq Version	XM_006500263.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tetratricopeptide repeat domain 21B (Ttc21b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500263.3

ORF Insert Sequence:

ATGGACTCCC AGGGGCTGAA GACGCTGATT AACTACTATT GTCAAGAGCG CTATTATCAC 
CACGTGCTCC TTGTTGCCAG TGAAGGGATG AAGAAGTATA GCAGCGACCC AGTCTTCCGA 
TTTTACCACG CCTACGGCAC ACTGATGGAG GGTAAAGCGC AAGAAGCCCT TCGGGAGTTT 
GAGGCCATTA AAAACAAACA AGACGTGTCA CTGTGTTCTC TGATGGCGCT GATGTATGTC 
CATAAAATGA GCCCCAATCC AGACAGAGAA GCTATCCTGG AATTAGATAC CAAAATGAAG 
GAGCAACGCA AGGAGGCTGG ACGCAAAGCC CTGTACCATG CGGGCCTGTT TTTATGGCAC 
ATTGGTCGTC ATGACAAGGC GAGAGAATAT ATTGACAGAA TGTCAAAAAT GCCACATGAT 
AGCAATGAGG GGCCGATTTT GAAAGCGTGG CTTGATATTA CAAGAGGGAA GGAACCTTAT 
GCTAAAAAAG CCCTGCGGTA TTTTGAGGAG GGATTACAAG ATGGAAATGA TATTTTCGCT 
CTTCTGGGTA AGGTTCTGTG CCTTGAGATT CGACAGAATT ATTCCGGAGC TCTGGAGACC 
GTGAGCCAGA TAATTGTAAA CTTTCCAAGC TTCCTTCCTG CCTTTGAGAA GAAAATGAAA 
TTACAACTGG CTTTACAGGA TTGGGATCAG ACAGTGGAGA CAGCACAAAG GCTGCTGCTT 
CAAGACAATC ACAACGTGGA GGCGCTGAGG ATGCTGGCTC TGTATTATCT GTGTAGGGAA 
GGGGACGTAG AGAAGGCTGC TACCAAGCTG GAAAATTTAG GAAATGCATT GGATGTCATG 
GAACCACAGA ATGCTCAACT TTTTTATAAG ATTACACTAG CCTTCAGCAG AACGTGTGGA 
CGTAATCAAC TCATTCTCCA GAAAGTTCAA AGTTTCCTAG AAAAAGCATT TAGTTTAACT 
CCCCAGCAAG CAGAAATTGC TACAGAGCTC GGCTACCAAA TGATTCTCCA AGGCAAGGTC 
AAGGAGGCCT GGAAGTGGTA CAGGACCGCC ATGACGCTGA ATGAGAGCAA CATCTCTGCT 
GTCACCGGAC TTATCCGATG TCAGTTAATA GAGGGGCAGT TGCAAGATGC AGACCAGCAG 
TTGGAGTTCT TCAGTGAATT CCAGCAGTCA ATGGGGAAAT CTGCGGAATT AATGTATTTG 
CATGCGGTTC TTGCTACGAA AAAAAATAAT CGTCAGGATG AAGTTATAAA TTTGTTGAAT 
GATGTTGTGA ATACTCACTT TTCACACTTG GAAGACCTCC CGCTCGGCAT ACAGTATTTT 
GAGAAGCTCA ACCCTGACTT CTTACTAGAA GTTGTTACCG AGTATCTGAA TCTCTGTCCA 
ATTCAGCCTG CAGGTCCTGG ACAACCTCTT TCTCCAGTTC TCAGACGTTG TTCTTCAGTC 
CTGGAGACGA TTATAAGAAG TGTACCAGGT CTTCCACAAG CTGTCTTCCT AATGGCCAAA 
GTGAAGTATT TGTCAGGTGA CACTGAAGCG GCATACAACA ATCTGCAGCA CTGCCTCGAG 
CACAGCCCCT CCTATGCGGA GGCTCACCTC CTGATGGCAC AGGTGTACCT GTCCCAAGAC 
AAAGTCAAGC TGTGTTCGCA GTCGCTTGAA CTTTGTCTGA GCTACAATTT TAATGTGAGA 
GAGTACCCAT TATATCATTT AATCAAAGCA CAGTCACAAA AGAAAATGGG GGAAGTAGCA 
GAAGCAATTA AAACTCTGCA CATGGCAATG AACCTGCCAG GAATGAGGAG AAGCAGAGCC 
TCCTCGAAAT CAAAGCACAG AACTGAGGTG GACGCGAGCC ATCGGTTATC CATCTTCCTG 
GAGTTGGTGG AGGTCCACCG CTTAAATGGA GAACAGCATG AGGCAGCAAA GGTTTTACAG 
GATGCCATCC ATGAGTTTTC GGGAACTTGT GAAGAATTAC GTGTCACTAT TGCTAATGCA 
GACCTGGCTC TGGCCCAAGG AGATACGGAT CGTGCATTAA GCATGCTTCG AAATGTCACA 
ACCGAACAGC CTTATTTTAT AGAGGCCAAA GAAAAGATGG CAGATATTTA TCTGAAGCAT 
AGGAAAGAGA AAATGTTGTA TATCACTTGT TACAGAGAAA TTGCTGAGAG AATGCCCAGT 
CCCCGGTCTT TCCTGCTCCT CGGCGATGCG TACATGAACA TTCAGGAGCC AGAAGAAGCC 
ATAGTGGCCT ATGAGCAAGC ACTCAATCAG AACCCCAAAG ATGGAACACT GGCAAGAAAA 
ATTGGAAAAG CACTTGTCAA AACTCACAAT TACTCAAAGG CAATCACCTA TTATGAAGCG 
GCTCTGAAAA GTGGACAGCA GAATTGCCTT TGCTATGACC TGGCCGAGCT GCTACTGAGA 
CTGAAGCTGT ACGAGAAAGC AGAGAAAGTG CTCCAGCACT CTCTAGCTCA TGAGCCTGTA 
AGTGAGTTGT CAGCTCTCAT GGTGGATGGG CGGTCCCAAG TCCTTCTTGC AAAAGTTTAC 
TCTAAAATGG AGAGACCCAG CGACGCGATT GCCGCATTAC AGCAGGCTCG AGAGCTGCAG 
GCTCGGATAT TGAAGCGAGT TCAGATGGAG CAGCCAGATG CAGTTCCTTC ACAGAAACAC 
TTCGCAGCTG AAATTTGTGC AGAGATTGCG AAACACTCCG CCGCTCAGCG AGACTATGAG 
AAAGCAATTA CATTTTATAG AGAAGCCCTG GTTCATTGTG AAACAGACAG TAAGATAATG 
TTGGAACTGG CGCAGTTATA CCTGGCCCAA GAAGACCTTG ACGCCTCCCT GCGACACTGT 
GCGCTGCTTC TCCAGAGGGA CCAGGACAAT GAACCTGCCA CCATGTTGAT GGCCGACCTC 
ATGTTCAGAA AGCAAGACTA TGAACAAGCA GTGTATCATC TGCAGCAGCT TTTAGACCGG 
AAACCAGATA ATTTTATGAC TTTGTCCCGT TTGATTGATC TCCTGAGAAG ATGTGGGAAA 
CTTGAGGATG TTCCGAGATT TTTCTTGATG GCTGAGAAAC ACAACTCCAG AACAAAATTG 
GAGCCAGGAT TTCAGTACTG TAAAGGATTA CATTTTTGGT ATACTGGAGA ACCAAATGAT 
GCCCTTCGAC ATTTTAATAA AGCTCGGAAA GATAGTGACT GGGGTCAGAA TGCACTTTAT 
AATATGATAG AAATCTGCCT AAATCCAGAC AATGAAACTA TTGGAGGTGA AGTGTTTGAA 
AACCTGAATG GAGACCTGGG CACTTCCCCT GAGAAGCAGG AGTCTGTGCA GTTAGCAGTG 
AGGACAGCAG AAAAGCTCCT TAAGGAACTG AAGCCTCAGA CCGTCCAGGG CCGCCTGCAA 
CTCCGCATAA TGGAAAACTG CTGCTTGATG GCCACCAAGC AGAAGTCCAG TGTGGAGCAG 
GCACTGAACA CCTTCACCGA AATCGCAGCC TCCGAGGATC ATATCCCAGC TCTCTTGGGA 
ATGGCAACGG CTTATATGAT CTTGAAACAG ACTCCAAAAG CCAGAAACCA GCTGAAGCGG 
ATTGCAAAAA TGCCTTGGAA TCCTATTGAA GCCGAGGATC TGGAGAAGAG CTGGCTGCTG 
CTTGCTGATA TTTATATCCA GTCAGCAAAA TATGACATGG CAGAGGAATT ACTAAAGCGG 
TGTCTCTGCC ATAACCGGTC CTGCTGCAAA GCTTATGAAT ATATGGGGTA CATTATGGAG 
AAAGAGCAAG CATACACAGA TGCGGCCTTC AACTATGAGA TGGCATGGAA ACATAGCAAC 
CAGACAAATC CTGCAGTGGG ATACAAATTG GCATTTAATT ACCTAAAAGC AAAACGATAT 
GTGGATGCAA TTGACGTATG TCACCAGGTT CTTGAAGCAC ATCCAACTTA CCCCAAAATC 
AGAAAGGATA TACTTGATAA GGCGCGTGCA TCTTTAAGAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A tissue-specific role for intraflagellar transport genes during craniofacial development.
PloS one12(3)e0174206(2017)
Schock EN,Struve JN,Chang CF,Williams TJ,Snedeker J,Attia AC,Stottmann RW,Brugmann SA

Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development.
PloS one12(3)e0173258(2017)
Snedeker J,Schock EN,Struve JN,Chang CF,Cionni M,Tran PV,Brugmann SA,Stottmann RW

Dysfunction of intraflagellar transport-A causes hyperphagia-induced obesity and metabolic syndrome.
Disease models & mechanisms9(7)789-98(2016 07)
Jacobs DT,Silva LM,Allard BA,Schonfeld MP,Chatterjee A,Talbott GC,Beier DR,Tran PV

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Ttc21b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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