Med25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med25
Entrez Gene ID	75613
Full Name	mediator complex subunit 25
Synonyms	2610034E13Rik,2610529E18Rik,ESTM2
General protein information	Preferred Names mediator complex subunit 25 Names mediator of RNA polymerase II transcription subunit 25 mediator of RNA polymerase II transcription, subunit 9 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B3\|7 28.95 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_029365.2	NP_083641.1	mediator of RNA polymerase II transcription subunit 25
XM_017312368.1	XP_017167857.1	mediator of RNA polymerase II transcription subunit 25 isoform X6
NM_001331208.1	NP_001318137.1	mediator of RNA polymerase II transcription subunit 25 isoform 4
XM_006541265.3	XP_006541328.1	mediator of RNA polymerase II transcription subunit 25 isoform X6
XM_030243067.1	XP_030098927.1	mediator of RNA polymerase II transcription subunit 25 isoform X7
XM_030243071.1	XP_030098931.1	mediator of RNA polymerase II transcription subunit 25 isoform X10
XM_030243073.1	XP_030098933.1	mediator of RNA polymerase II transcription subunit 25 isoform X12
NM_001331207.1	NP_001318136.1	mediator of RNA polymerase II transcription subunit 25 isoform 3
XM_017312366.1	XP_017167855.1	mediator of RNA polymerase II transcription subunit 25 isoform X1
XM_006541263.1	XP_006541326.1	mediator of RNA polymerase II transcription subunit 25 isoform X4
XM_017312367.1	XP_017167856.1	mediator of RNA polymerase II transcription subunit 25 isoform X5
NM_001331206.1	NP_001318135.1	mediator of RNA polymerase II transcription subunit 25 isoform 2
XM_017312366.2	XP_017167855.1	mediator of RNA polymerase II transcription subunit 25 isoform X1
XM_030243066.1	XP_030098926.1	mediator of RNA polymerase II transcription subunit 25 isoform X2
XM_017312367.2	XP_017167856.1	mediator of RNA polymerase II transcription subunit 25 isoform X4
XM_006541264.2	XP_006541327.1	mediator of RNA polymerase II transcription subunit 25 isoform X5
XM_030243072.1	XP_030098932.1	mediator of RNA polymerase II transcription subunit 25 isoform X11
XM_006541264.1	XP_006541327.1	mediator of RNA polymerase II transcription subunit 25 isoform X6
XM_006541265.2	XP_006541328.1	mediator of RNA polymerase II transcription subunit 25 isoform X7
NM_029365.3	NP_083641.1	mediator of RNA polymerase II transcription subunit 25 isoform 1
XM_006541263.2	XP_006541326.1	mediator of RNA polymerase II transcription subunit 25 isoform X3
XM_030243068.1	XP_030098928.1	mediator of RNA polymerase II transcription subunit 25 isoform X8
XM_030243069.1	XP_030098929.1	mediator of RNA polymerase II transcription subunit 25 isoform X9
XM_030243070.1	XP_030098930.1	mediator of RNA polymerase II transcription subunit 25 isoform X10

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MED25	XP_005616347.1
Pan troglodytes (chimpanzee)	MED25	XP_003316596.2
Mus musculus (house mouse)	Med25	NP_083641.1
Rattus norvegicus (Norway rat)	Med25	NP_001163897.1
Bos taurus (cattle)	MED25	NP_001075914.1
Homo sapiens (human)	MED25	NP_112235.2
	MED25	XP_001115505.1
Xenopus tropicalis (tropical clawed frog)	med25	NP_001096266.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

MED25 is a mediator component of HNF4?-driven transcription leading to insulin secretion in pancreatic beta-cells.
Han EH, Rha GB, Chi YI
PloS one7(8)e44007(2012)

Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25.
Nakamura Y, Yamamoto K, He X, Otsuki B, Kim Y, Murao H, Soeda T, Tsumaki N, Deng JM, Zhang Z, Behringer RR, Crombrugghe Bd, Postlethwait JH, Warman ML, Nakamura T, Akiyama H
Nature communications2251(2011)

Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.
Leal A, Huehne K, Bauer F, Sticht H, Berger P, Suter U, Morera B, Del Valle G, Lupski JR, Ekici A, Pasutto F, Endele S, Barrantes R, Berghoff C, Berghoff M, Neund?rfer B, Heuss D, Dorn T, Young P, Santolin L, Uhlmann T, Meisterernst M, Sereda MW, Sereda M, Stassart RM, Meyer zu Horste G, Nave KA, Reis A, Rautenstrauss B
Neurogenetics10(4)275-87(2009 Oct)

MED25 is distinct from TRAP220/MED1 in cooperating with CBP for retinoid receptor activation.
Lee HK, Park UH, Kim EJ, Um SJ
The EMBO journal26(15)3545-57(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the identification of MED25 as one of the HNF4alpha binding partners in pancreatic ss-cells leading to insulin secretion which is impaired in MODY patients
Title: MED25 is a mediator component of HNF4?-driven transcription leading to insulin secretion in pancreatic beta-cells.

Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease.
Title: Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.

The following Med25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu39093	NM_001331208.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu39092	XM_017312368.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu06878	NM_029365.3 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$440.30 $629.00
OMu83365	NM_001331207.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu83367	NM_001331206.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu39090	XM_006541263.2 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu39091	XM_006541264.2 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu39092	XM_006541265.3 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu39093	XM_030243073.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu82246	XM_017312366.2 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu82247	XM_017312367.2 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu89654	XM_030243066.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu89655	XM_030243067.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu89656	XM_030243068.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu89657	XM_030243069.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu89658	XM_030243071.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu89658	XM_030243070.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu89659	XM_030243072.1 Latest version!	Mus musculus mediator complex subunit 25 (Med25), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu82246	XM_017312366.1	Mus musculus mediator complex subunit 25 (Med25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu82247	XM_017312367.1	Mus musculus mediator complex subunit 25 (Med25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu39091	XM_006541264.1	Mus musculus mediator complex subunit 25 (Med25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu39092	XM_006541265.2	Mus musculus mediator complex subunit 25 (Med25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu06878	NM_029365.2	Mus musculus mediator complex subunit 25 (Med25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$440.30 $629.00
OMu39090	XM_006541263.1	Mus musculus mediator complex subunit 25 (Med25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med25 ( NM_001331208.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001318137.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu39093
Clone ID Related Accession (Same CDS sequence)	NM_001331208.1 , XM_030243073.1
Accession Version	NM_001331208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158231.2. On Sep 13, 2016 this sequence version replaced XM_006541266.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC071206.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGCTACAGCT CCGTGCACCA 
CAGCCTCAGC CTCAGGGTGC CGTGGGAGCT TCTGCGGCTA CAGGGCAGCC CCAGCCCCAA 
GGTGCTACCC AGGCCCCCAC AGGCGCACCT CAAGGTCCTC CTGGAGCAGC CCCTGGCCCA 
CCTCCTTCTG GACCCATCCT GCGGCCCCAG AACCCTGGGG CCAACCCCCA GCTGCGGAGC 
CTTCTCCTTA ACCCAGCACC GCCCCAGACT GGGGTGCCCC CGCCCCAGGC CTCCCTACAC 
CACCTACAGC CTCCAGGGGC CCCTACATTG CTGCCACCAC ATCAGAGCAT GGGGCAGCCC 
CAGCTGGGGC CGCAGCTCCT GCATCCTCCA CCTGCCCAGT CATGGCCCAC ACAGCTTCCC 
CAGCGGGCGC CACTGCCAGG TCAGATGCTG CTGAGCGGGG GTCCCAGGGG CCCGGTCCCC 
CAGCCGGGCC TGCAGCCCAG CGTCATGGAG GACGACATCC TCATGGACCT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318137.1
CDS	410..2266
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	NM_001331208.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331208.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGCTACAGCT CCGTGCACCA 
CAGCCTCAGC CTCAGGGTGC CGTGGGAGCT TCTGCGGCTA CAGGGCAGCC CCAGCCCCAA 
GGTGCTACCC AGGCCCCCAC AGGCGCACCT CAAGGTCCTC CTGGAGCAGC CCCTGGCCCA 
CCTCCTTCTG GACCCATCCT GCGGCCCCAG AACCCTGGGG CCAACCCCCA GCTGCGGAGC 
CTTCTCCTTA ACCCAGCACC GCCCCAGACT GGGGTGCCCC CGCCCCAGGC CTCCCTACAC 
CACCTACAGC CTCCAGGGGC CCCTACATTG CTGCCACCAC ATCAGAGCAT GGGGCAGCCC 
CAGCTGGGGC CGCAGCTCCT GCATCCTCCA CCTGCCCAGT CATGGCCCAC ACAGCTTCCC 
CAGCGGGCGC CACTGCCAGG TCAGATGCTG CTGAGCGGGG GTCCCAGGGG CCCGGTCCCC 
CAGCCGGGCC TGCAGCCCAG CGTCATGGAG GACGACATCC TCATGGACCT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_017312368.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167857.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu39092
Clone ID Related Accession (Same CDS sequence)	XM_017312368.1 , XM_006541265.3 , XM_006541265.2
Accession Version	XM_017312368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGTGGCCA AACGAAAGAG AGAAGGAGAG 
GGCCGCGTGT TTCGAGAAAA GTGGGAGCGA GACTATTTCT TTGTGGAAGT GAAGAGCATG 
CCTACGTGCT TAATATGCAA AAAAAACGTG TCAGTGCTGA AAGAGTACAA CCTGAAGCGC 
CACTATGAGT CCCAGCACAG TAAGAGCTAC GATCAGTACA CAGCGCAGAG CCGCGACACA 
CTCCTGCAGG AACTGAAAAG GGCCCTGAGA GCCAGCGAGG CGCTGGAGAG CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167857.1
CDS	405..2504
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_017312368.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312368.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGTGGCCA AACGAAAGAG AGAAGGAGAG 
GGCCGCGTGT TTCGAGAAAA GTGGGAGCGA GACTATTTCT TTGTGGAAGT GAAGAGCATG 
CCTACGTGCT TAATATGCAA AAAAAACGTG TCAGTGCTGA AAGAGTACAA CCTGAAGCGC 
CACTATGAGT CCCAGCACAG TAAGAGCTAC GATCAGTACA CAGCGCAGAG CCGCGACACA 
CTCCTGCAGG AACTGAAAAG GGCCCTGAGA GCCAGCGAGG CGCTGGAGAG CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_006541265.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541328.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X7, mRNA.

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CloneID	OMu39092
Clone ID Related Accession (Same CDS sequence)	XM_017312368.1 , XM_006541265.3 , XM_006541265.2
Accession Version	XM_006541265.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006541265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGTGGCCA AACGAAAGAG AGAAGGAGAG 
GGCCGCGTGT TTCGAGAAAA GTGGGAGCGA GACTATTTCT TTGTGGAAGT GAAGAGCATG 
CCTACGTGCT TAATATGCAA AAAAAACGTG TCAGTGCTGA AAGAGTACAA CCTGAAGCGC 
CACTATGAGT CCCAGCACAG TAAGAGCTAC GATCAGTACA CAGCGCAGAG CCGCGACACA 
CTCCTGCAGG AACTGAAAAG GGCCCTGAGA GCCAGCGAGG CGCTGGAGAG CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541328.1
CDS	381..2480
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_006541265.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541265.2

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGTGGCCA AACGAAAGAG AGAAGGAGAG 
GGCCGCGTGT TTCGAGAAAA GTGGGAGCGA GACTATTTCT TTGTGGAAGT GAAGAGCATG 
CCTACGTGCT TAATATGCAA AAAAAACGTG TCAGTGCTGA AAGAGTACAA CCTGAAGCGC 
CACTATGAGT CCCAGCACAG TAAGAGCTAC GATCAGTACA CAGCGCAGAG CCGCGACACA 
CTCCTGCAGG AACTGAAAAG GGCCCTGAGA GCCAGCGAGG CGCTGGAGAG CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( NM_029365.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083641.1 Mus musculus mediator complex subunit 25 (Med25), mRNA.

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CloneID	OMu06878
Clone ID Related Accession (Same CDS sequence)	NM_029365.2 , NM_029365.3
Accession Version	NM_029365.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	mediator of RNA polymerase II transcription subunit 25
Comment

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_083641.1

Translation

MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	NM_029365.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029365.2

ORF Insert Sequence:

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( NM_029365.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083641.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant 1, mRNA.

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CloneID	OMu06878
Clone ID Related Accession (Same CDS sequence)	NM_029365.2 , NM_029365.3
Accession Version	NM_029365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158231.2. On Sep 13, 2016 this sequence version replaced NM_029365.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC031138.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083641.1
CDS	61..2298
Translation	MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	NM_029365.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029365.3

ORF Insert Sequence:

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( NM_001331207.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001318136.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu83365
Clone ID Related Accession (Same CDS sequence)	NM_001331207.1
Accession Version	NM_001331207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158231.2. On Sep 13, 2016 this sequence version replaced XM_006541261.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK090026.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG TGGCCAAACG AAAGAGAGAA 
GGAGAGGGCC GCGTGTTTCG AGAAAAGTGG GAGCGAGACT ATTTCTTTGT GGAAGTGAAG 
AGCATGCCTA CGTGCTTAAT ATGCAAAAAA AACGTGTCAG TGCTGAAAGA GTACAACCTG 
AAGCGCCACT ATGAGTCCCA GCACAGTAAG AGCTACGATC AGTACACAGC GCAGAGCCGC 
GACACACTCC TGCAGGAACT GAAAAGGGCC CTGAGAGCCA GCGAGGCGCT GGAGAGCCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318136.1
CDS	61..2466
Translation	MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	NM_001331207.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331207.1

ORF Insert Sequence:

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG TGGCCAAACG AAAGAGAGAA 
GGAGAGGGCC GCGTGTTTCG AGAAAAGTGG GAGCGAGACT ATTTCTTTGT GGAAGTGAAG 
AGCATGCCTA CGTGCTTAAT ATGCAAAAAA AACGTGTCAG TGCTGAAAGA GTACAACCTG 
AAGCGCCACT ATGAGTCCCA GCACAGTAAG AGCTACGATC AGTACACAGC GCAGAGCCGC 
GACACACTCC TGCAGGAACT GAAAAGGGCC CTGAGAGCCA GCGAGGCGCT GGAGAGCCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( NM_001331206.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001318135.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83367
Clone ID Related Accession (Same CDS sequence)	NM_001331206.1
Accession Version	NM_001331206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB524888.1, AK158238.1, AK149867.1, AK165760.1 and AW495583.1. On Sep 13, 2016 this sequence version replaced XM_006541262.2. ##Evidence-Data-START## Transcript exon combination :: AK158238.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GCAGGCAGGC ACTGTAACCC CAGGTGGGGT CAATGGTCCT 
TCAGCAGCCC AGCTTGGGGG TCCTGCACTT GGTGGGCAGC AGTCAGTATC CAACAAGCTC 
CTAGCCTGGA GTGGAGTCCT CGAGTGGCAG GAGAAACCCA AGCCTGCATC TGTGGATGCC 
AACACCAAGC TGACGAGGTC TCTGCCTTGC CAGGTCTATG TGAACCATGG AGAAAACCTG 
AAGACTGAGC AGTGGCCCCA GAAACTGATC ATGCAGCTTA TCCCTCAGCA GCTGCTGACC 
ACCCTGGGTC CGCTGTTCCG GAACTCAAGA ATGGTTCAGT TCCACTTCAC CAACAAGGAC 
CTGGAGTCCC TCAAAGGCCT CTACCGCATC ATGGGCAATG GCTTTGCGGG CTGCGTGCAT 
TTCCCGCACA CGGCCCCATG CGAGGTGCGC GTGCTTATGC TCCTGTACTC GTCTAAGAAG 
AAGATCTTCA TGGGCCTCAT CCCCTACGAC CAGAGCGGCT TCGTCAATGG CATCCGCCAG 
GTCATCACCA ACCACAAGCA GGTCCAGCAG CAGAAGCTGG AGCAACAGCG CGGGCTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GTGGCCAAAC GAAAGAGAGA AGGAGAGGGC 
CGCGTGTTTC GAGAAAAGTG GGAGCGAGAC TATTTCTTTG TGGAAGTGAA GAGCATGCCT 
ACGTGCTTAA TATGCAAAAA AAACGTGTCA GTGCTGAAAG AGTACAACCT GAAGCGCCAC 
TATGAGTCCC AGCACAGTAA GAGCTACGAT CAGTACACAG CGCAGAGCCG CGACACACTC 
CTGCAGGAAC TGAAAAGGGC CCTGAGAGCC AGCGAGGCGC TGGAGAGCCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318135.1
CDS	61..2397
Translation	MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	NM_001331206.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331206.1

ORF Insert Sequence:

ATGGTCCCCG GATCCGAAGG CCCGGCGCGG GCCGGGGGCC TGGTCGCCGA TGTGGTGTTT 
GTGATCGAGG GGACAGCAAA CCTGGGGCCG TACTTCGAGG AGCTCCGCAA GCACTACCTG 
CTGCCTGCCA TAGAGTACTT CAACGGGGGA CCCCCTGCAG AGACGGACTT TGGGGGAGAC 
TATGGTGGAA CCCAGTACAG CCTTGTGGTG TTCAACACCG TGGACTGCGC TCCAGAGTCC 
TATGTACAAT GTCACGCACC TACCAGCAGT GCCTATGAGT TTGTCACCTG GCTCGATGGC 
ATCAAGTTCA TGGGCGGTGG TGGCGAGAGC TGTAGTCTCA TCGCTGAAGG TCTGAGCACT 
GCCCTGCAGC TGTTCGATGA CTTTAAGAAG ATGCGTGAAC AGATTGGCCA GACCCACCGA 
GTCTGTCTCC TCATCTGCAA CTCACCCCCG TACCTCCTGC CCGCCGTGGA GAGCACCACC 
TACTCAGGCT GCACTACCGA GAGCCTGGTG CAGAAAATAG GAGAGCGAGG CATCCACTTC 
TCCATCGTTT CTCCGAGAAA GCTGCCAGCC CTCCGGCTTC TGTTTGAGAA GGCGGCTCCT 
CCTGCCCTAC TGGAGCCTTT GCAGCAACCA GCAGACGTCA GCCAGGACCC CAGGCACATG 
GTGCTTGTGC GTGGGCTCGT GCTGCCTGTT GGTGGCAGCT CGACCTCAGG CTCTCTGCAG 
ACCAAGCAAG CAGTCCCCTT GCCACCAGCT CCAGCTTCTG CTGCCACGCT GTCAGCAGCG 
CCCCCGCAGG CTCTGCCCCC TGTCCCACCA CAGTACCAGG TTCCTGGGAA CCTAAGTGCT 
GCTCAGGTGG CTGCGCAGAA TGCTGTGGAG GCTGCCAAGA GCCAGAAGGC CGGGCTGGGC 
CCACGCTTCT CACCCATCAA CCCTCTCCAG CAAGCTGCTC CAGGAGTGGG GCCCCCCTTC 
AGCCAGGCCC CAGCACCCCC ATTGGCTCCT GTGCCCCCTG GAGCTCCAAA GCCACCTCCT 
GCCTCACAGC CTAGCCTAGT CTCCACCGTG GCCCCCGGAC CAGTCCTGGC AGCTCCTGCG 
CAGCCTGGGG CGCCGTCTCT GCAGGCAGGC ACTGTAACCC CAGGTGGGGT CAATGGTCCT 
TCAGCAGCCC AGCTTGGGGG TCCTGCACTT GGTGGGCAGC AGTCAGTATC CAACAAGCTC 
CTAGCCTGGA GTGGAGTCCT CGAGTGGCAG GAGAAACCCA AGCCTGCATC TGTGGATGCC 
AACACCAAGC TGACGAGGTC TCTGCCTTGC CAGGTCTATG TGAACCATGG AGAAAACCTG 
AAGACTGAGC AGTGGCCCCA GAAACTGATC ATGCAGCTTA TCCCTCAGCA GCTGCTGACC 
ACCCTGGGTC CGCTGTTCCG GAACTCAAGA ATGGTTCAGT TCCACTTCAC CAACAAGGAC 
CTGGAGTCCC TCAAAGGCCT CTACCGCATC ATGGGCAATG GCTTTGCGGG CTGCGTGCAT 
TTCCCGCACA CGGCCCCATG CGAGGTGCGC GTGCTTATGC TCCTGTACTC GTCTAAGAAG 
AAGATCTTCA TGGGCCTCAT CCCCTACGAC CAGAGCGGCT TCGTCAATGG CATCCGCCAG 
GTCATCACCA ACCACAAGCA GGTCCAGCAG CAGAAGCTGG AGCAACAGCG CGGGCTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GTGGCCAAAC GAAAGAGAGA AGGAGAGGGC 
CGCGTGTTTC GAGAAAAGTG GGAGCGAGAC TATTTCTTTG TGGAAGTGAA GAGCATGCCT 
ACGTGCTTAA TATGCAAAAA AAACGTGTCA GTGCTGAAAG AGTACAACCT GAAGCGCCAC 
TATGAGTCCC AGCACAGTAA GAGCTACGAT CAGTACACAG CGCAGAGCCG CGACACACTC 
CTGCAGGAAC TGAAAAGGGC CCTGAGAGCC AGCGAGGCGC TGGAGAGCCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_006541263.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541326.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu39090
Clone ID Related Accession (Same CDS sequence)	XM_006541263.1 , XM_006541263.2
Accession Version	XM_006541263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006541263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541326.1
CDS	165..2582
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_006541263.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541263.2

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_006541264.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541327.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu39091
Clone ID Related Accession (Same CDS sequence)	XM_006541264.2 , XM_006541264.1
Accession Version	XM_006541264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006541264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGGC AGGCACTGTA ACCCCAGGTG GGGTCAATGG TCCTTCAGCA 
GCCCAGCTTG GGGGTCCTGC ACTTGGTGGG CAGCAGTCAG TATCCAACAA GCTCCTAGCC 
TGGAGTGGAG TCCTCGAGTG GCAGGAGAAA CCCAAGCCTG CATCTGTGGA TGCCAACACC 
AAGCTGACGA GGTCTCTGCC TTGCCAGGTC TATGTGAACC ATGGAGAAAA CCTGAAGACT 
GAGCAGTGGC CCCAGAAACT GATCATGCAG CTTATCCCTC AGCAGCTGCT GACCACCCTG 
GGTCCGCTGT TCCGGAACTC AAGAATGGTT CAGTTCCACT TCACCAACAA GGACCTGGAG 
TCCCTCAAAG GCCTCTACCG CATCATGGGC AATGGCTTTG CGGGCTGCGT GCATTTCCCG 
CACACGGCCC CATGCGAGGT GCGCGTGCTT ATGCTCCTGT ACTCGTCTAA GAAGAAGATC 
TTCATGGGCC TCATCCCCTA CGACCAGAGC GGCTTCGTCA ATGGCATCCG CCAGGTCATC 
ACCAACCACA AGCAGGTCCA GCAGCAGAAG CTGGAGCAAC AGCGCGGGCT ACAGCTCCGT 
GCACCACAGC CTCAGCCTCA GGGTGCCGTG GGAGCTTCTG CGGCTACAGG GCAGCCCCAG 
CCCCAAGGTG CTACCCAGGC CCCCACAGGC GCACCTCAAG GTCCTCCTGG AGCAGCCCCT 
GGCCCACCTC CTTCTGGACC CATCCTGCGG CCCCAGAACC CTGGGGCCAA CCCCCAGCTG 
CGGAGCCTTC TCCTTAACCC AGCACCGCCC CAGACTGGGG TGCCCCCGCC CCAGGCCTCC 
CTACACCACC TACAGCCTCC AGGGGCCCCT ACATTGCTGC CACCACATCA GAGCATGGGG 
CAGCCCCAGC TGGGGCCGCA GCTCCTGCAT CCTCCACCTG CCCAGTCATG GCCCACACAG 
CTTCCCCAGC GGGCGCCACT GCCAGGTCAG ATGCTGCTGA GCGGGGGTCC CAGGGGCCCG 
GTCCCCCAGC CGGGCCTGCA GCCCAGCGTC ATGGAGGACG ACATCCTCAT GGACCTCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541327.1
CDS	165..2507
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_006541264.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541264.2

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGGC AGGCACTGTA ACCCCAGGTG GGGTCAATGG TCCTTCAGCA 
GCCCAGCTTG GGGGTCCTGC ACTTGGTGGG CAGCAGTCAG TATCCAACAA GCTCCTAGCC 
TGGAGTGGAG TCCTCGAGTG GCAGGAGAAA CCCAAGCCTG CATCTGTGGA TGCCAACACC 
AAGCTGACGA GGTCTCTGCC TTGCCAGGTC TATGTGAACC ATGGAGAAAA CCTGAAGACT 
GAGCAGTGGC CCCAGAAACT GATCATGCAG CTTATCCCTC AGCAGCTGCT GACCACCCTG 
GGTCCGCTGT TCCGGAACTC AAGAATGGTT CAGTTCCACT TCACCAACAA GGACCTGGAG 
TCCCTCAAAG GCCTCTACCG CATCATGGGC AATGGCTTTG CGGGCTGCGT GCATTTCCCG 
CACACGGCCC CATGCGAGGT GCGCGTGCTT ATGCTCCTGT ACTCGTCTAA GAAGAAGATC 
TTCATGGGCC TCATCCCCTA CGACCAGAGC GGCTTCGTCA ATGGCATCCG CCAGGTCATC 
ACCAACCACA AGCAGGTCCA GCAGCAGAAG CTGGAGCAAC AGCGCGGGCT ACAGCTCCGT 
GCACCACAGC CTCAGCCTCA GGGTGCCGTG GGAGCTTCTG CGGCTACAGG GCAGCCCCAG 
CCCCAAGGTG CTACCCAGGC CCCCACAGGC GCACCTCAAG GTCCTCCTGG AGCAGCCCCT 
GGCCCACCTC CTTCTGGACC CATCCTGCGG CCCCAGAACC CTGGGGCCAA CCCCCAGCTG 
CGGAGCCTTC TCCTTAACCC AGCACCGCCC CAGACTGGGG TGCCCCCGCC CCAGGCCTCC 
CTACACCACC TACAGCCTCC AGGGGCCCCT ACATTGCTGC CACCACATCA GAGCATGGGG 
CAGCCCCAGC TGGGGCCGCA GCTCCTGCAT CCTCCACCTG CCCAGTCATG GCCCACACAG 
CTTCCCCAGC GGGCGCCACT GCCAGGTCAG ATGCTGCTGA GCGGGGGTCC CAGGGGCCCG 
GTCCCCCAGC CGGGCCTGCA GCCCAGCGTC ATGGAGGACG ACATCCTCAT GGACCTCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_006541265.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541328.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X6, mRNA.

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CloneID	OMu39092
Clone ID Related Accession (Same CDS sequence)	XM_017312368.1 , XM_006541265.3 , XM_006541265.2
Accession Version	XM_006541265.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006541265.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGTGGCCA AACGAAAGAG AGAAGGAGAG 
GGCCGCGTGT TTCGAGAAAA GTGGGAGCGA GACTATTTCT TTGTGGAAGT GAAGAGCATG 
CCTACGTGCT TAATATGCAA AAAAAACGTG TCAGTGCTGA AAGAGTACAA CCTGAAGCGC 
CACTATGAGT CCCAGCACAG TAAGAGCTAC GATCAGTACA CAGCGCAGAG CCGCGACACA 
CTCCTGCAGG AACTGAAAAG GGCCCTGAGA GCCAGCGAGG CGCTGGAGAG CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541328.1
CDS	404..2503
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_006541265.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541265.3

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGTGGCCA AACGAAAGAG AGAAGGAGAG 
GGCCGCGTGT TTCGAGAAAA GTGGGAGCGA GACTATTTCT TTGTGGAAGT GAAGAGCATG 
CCTACGTGCT TAATATGCAA AAAAAACGTG TCAGTGCTGA AAGAGTACAA CCTGAAGCGC 
CACTATGAGT CCCAGCACAG TAAGAGCTAC GATCAGTACA CAGCGCAGAG CCGCGACACA 
CTCCTGCAGG AACTGAAAAG GGCCCTGAGA GCCAGCGAGG CGCTGGAGAG CCAGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243073.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098933.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X14, mRNA.

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CloneID	OMu39093
Clone ID Related Accession (Same CDS sequence)	NM_001331208.1 , XM_030243073.1
Accession Version	XM_030243073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGCTACAGCT CCGTGCACCA 
CAGCCTCAGC CTCAGGGTGC CGTGGGAGCT TCTGCGGCTA CAGGGCAGCC CCAGCCCCAA 
GGTGCTACCC AGGCCCCCAC AGGCGCACCT CAAGGTCCTC CTGGAGCAGC CCCTGGCCCA 
CCTCCTTCTG GACCCATCCT GCGGCCCCAG AACCCTGGGG CCAACCCCCA GCTGCGGAGC 
CTTCTCCTTA ACCCAGCACC GCCCCAGACT GGGGTGCCCC CGCCCCAGGC CTCCCTACAC 
CACCTACAGC CTCCAGGGGC CCCTACATTG CTGCCACCAC ATCAGAGCAT GGGGCAGCCC 
CAGCTGGGGC CGCAGCTCCT GCATCCTCCA CCTGCCCAGT CATGGCCCAC ACAGCTTCCC 
CAGCGGGCGC CACTGCCAGG TCAGATGCTG CTGAGCGGGG GTCCCAGGGG CCCGGTCCCC 
CAGCCGGGCC TGCAGCCCAG CGTCATGGAG GACGACATCC TCATGGACCT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098933.1
CDS	404..2260
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_030243073.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243073.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGCTACAGCT CCGTGCACCA 
CAGCCTCAGC CTCAGGGTGC CGTGGGAGCT TCTGCGGCTA CAGGGCAGCC CCAGCCCCAA 
GGTGCTACCC AGGCCCCCAC AGGCGCACCT CAAGGTCCTC CTGGAGCAGC CCCTGGCCCA 
CCTCCTTCTG GACCCATCCT GCGGCCCCAG AACCCTGGGG CCAACCCCCA GCTGCGGAGC 
CTTCTCCTTA ACCCAGCACC GCCCCAGACT GGGGTGCCCC CGCCCCAGGC CTCCCTACAC 
CACCTACAGC CTCCAGGGGC CCCTACATTG CTGCCACCAC ATCAGAGCAT GGGGCAGCCC 
CAGCTGGGGC CGCAGCTCCT GCATCCTCCA CCTGCCCAGT CATGGCCCAC ACAGCTTCCC 
CAGCGGGCGC CACTGCCAGG TCAGATGCTG CTGAGCGGGG GTCCCAGGGG CCCGGTCCCC 
CAGCCGGGCC TGCAGCCCAG CGTCATGGAG GACGACATCC TCATGGACCT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_017312366.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167855.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X1, mRNA.

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CloneID	OMu82246
Clone ID Related Accession (Same CDS sequence)	XM_017312366.1 , XM_017312366.2
Accession Version	XM_017312366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG TGGCCAAACG AAAGAGAGAA 
GGAGAGGGCC GCGTGTTTCG AGAAAAGTGG GAGCGAGACT ATTTCTTTGT GGAAGTGAAG 
AGCATGCCTA CGTGCTTAAT ATGCAAAAAA AACGTGTCAG TGCTGAAAGA GTACAACCTG 
AAGCGCCACT ATGAGTCCCA GCACAGTAAG AGCTACGATC AGTACACAGC GCAGAGCCGC 
GACACACTCC TGCAGGAACT GAAAAGGGCC CTGAGAGCCA GCGAGGCGCT GGAGAGCCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167855.1
CDS	165..2750
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_017312366.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312366.2

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG TGGCCAAACG AAAGAGAGAA 
GGAGAGGGCC GCGTGTTTCG AGAAAAGTGG GAGCGAGACT ATTTCTTTGT GGAAGTGAAG 
AGCATGCCTA CGTGCTTAAT ATGCAAAAAA AACGTGTCAG TGCTGAAAGA GTACAACCTG 
AAGCGCCACT ATGAGTCCCA GCACAGTAAG AGCTACGATC AGTACACAGC GCAGAGCCGC 
GACACACTCC TGCAGGAACT GAAAAGGGCC CTGAGAGCCA GCGAGGCGCT GGAGAGCCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_017312367.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167856.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X4, mRNA.

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CloneID	OMu82247
Clone ID Related Accession (Same CDS sequence)	XM_017312367.1 , XM_017312367.2
Accession Version	XM_017312367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GCTACAGCTC 
CGTGCACCAC AGCCTCAGCC TCAGGGTGCC GTGGGAGCTT CTGCGGCTAC AGGGCAGCCC 
CAGCCCCAAG GTGCTACCCA GGCCCCCACA GGCGCACCTC AAGGTCCTCC TGGAGCAGCC 
CCTGGCCCAC CTCCTTCTGG ACCCATCCTG CGGCCCCAGA ACCCTGGGGC CAACCCCCAG 
CTGCGGAGCC TTCTCCTTAA CCCAGCACCG CCCCAGACTG GGGTGCCCCC GCCCCAGGCC 
TCCCTACACC ACCTACAGCC TCCAGGGGCC CCTACATTGC TGCCACCACA TCAGAGCATG 
GGGCAGCCCC AGCTGGGGCC GCAGCTCCTG CATCCTCCAC CTGCCCAGTC ATGGCCCACA 
CAGCTTCCCC AGCGGGCGCC ACTGCCAGGT CAGATGCTGC TGAGCGGGGG TCCCAGGGGC 
CCGGTCCCCC AGCCGGGCCT GCAGCCCAGC GTCATGGAGG ACGACATCCT CATGGACCTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167856.1
CDS	165..2510
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_017312367.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312367.2

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GCTACAGCTC 
CGTGCACCAC AGCCTCAGCC TCAGGGTGCC GTGGGAGCTT CTGCGGCTAC AGGGCAGCCC 
CAGCCCCAAG GTGCTACCCA GGCCCCCACA GGCGCACCTC AAGGTCCTCC TGGAGCAGCC 
CCTGGCCCAC CTCCTTCTGG ACCCATCCTG CGGCCCCAGA ACCCTGGGGC CAACCCCCAG 
CTGCGGAGCC TTCTCCTTAA CCCAGCACCG CCCCAGACTG GGGTGCCCCC GCCCCAGGCC 
TCCCTACACC ACCTACAGCC TCCAGGGGCC CCTACATTGC TGCCACCACA TCAGAGCATG 
GGGCAGCCCC AGCTGGGGCC GCAGCTCCTG CATCCTCCAC CTGCCCAGTC ATGGCCCACA 
CAGCTTCCCC AGCGGGCGCC ACTGCCAGGT CAGATGCTGC TGAGCGGGGG TCCCAGGGGC 
CCGGTCCCCC AGCCGGGCCT GCAGCCCAGC GTCATGGAGG ACGACATCCT CATGGACCTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243066.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098926.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu89654
Clone ID Related Accession (Same CDS sequence)	XM_030243066.1
Accession Version	XM_030243066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGGC AGGCACTGTA ACCCCAGGTG GGGTCAATGG TCCTTCAGCA 
GCCCAGCTTG GGGGTCCTGC ACTTGGTGGG CAGCAGTCAG TATCCAACAA GCTCCTAGCC 
TGGAGTGGAG TCCTCGAGTG GCAGGAGAAA CCCAAGCCTG CATCTGTGGA TGCCAACACC 
AAGCTGACGA GGTCTCTGCC TTGCCAGGTC TATGTGAACC ATGGAGAAAA CCTGAAGACT 
GAGCAGTGGC CCCAGAAACT GATCATGCAG CTTATCCCTC AGCAGCTGCT GACCACCCTG 
GGTCCGCTGT TCCGGAACTC AAGAATGGTT CAGTTCCACT TCACCAACAA GGACCTGGAG 
TCCCTCAAAG GCCTCTACCG CATCATGGGC AATGGCTTTG CGGGCTGCGT GCATTTCCCG 
CACACGGCCC CATGCGAGGT GCGCGTGCTT ATGCTCCTGT ACTCGTCTAA GAAGAAGATC 
TTCATGGGCC TCATCCCCTA CGACCAGAGC GGCTTCGTCA ATGGCATCCG CCAGGTCATC 
ACCAACCACA AGCAGGTCCA GCAGCAGAAG CTGGAGCAAC AGCGCGGGCT ACAGCTCCGT 
GCACCACAGC CTCAGCCTCA GGGTGCCGTG GGAGCTTCTG CGGCTACAGG GCAGCCCCAG 
CCCCAAGGTG CTACCCAGGC CCCCACAGGC GCACCTCAAG GTCCTCCTGG AGCAGCCCCT 
GGCCCACCTC CTTCTGGACC CATCCTGCGG CCCCAGAACC CTGGGGCCAA CCCCCAGCTG 
CGGAGCCTTC TCCTTAACCC AGCACCGCCC CAGACTGGGG TGCCCCCGCC CCAGGCCTCC 
CTACACCACC TACAGCCTCC AGGGGCCCCT ACATTGCTGC CACCACATCA GAGCATGGGG 
CAGCCCCAGC TGGGGCCGCA GCTCCTGCAT CCTCCACCTG CCCAGTCATG GCCCACACAG 
CTTCCCCAGC GGGCGCCACT GCCAGTGGCC AAACGAAAGA GAGAAGGAGA GGGCCGCGTG 
TTTCGAGAAA AGTGGGAGCG AGACTATTTC TTTGTGGAAG TGAAGAGCAT GCCTACGTGC 
TTAATATGCA AAAAAAACGT GTCAGTGCTG AAAGAGTACA ACCTGAAGCG CCACTATGAG 
TCCCAGCACA GTAAGAGCTA CGATCAGTAC ACAGCGCAGA GCCGCGACAC ACTCCTGCAG 
GAACTGAAAA GGGCCCTGAG AGCCAGCGAG GCGCTGGAGA GCCAGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098926.1
CDS	165..2675
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_030243066.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243066.1

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGGC AGGCACTGTA ACCCCAGGTG GGGTCAATGG TCCTTCAGCA 
GCCCAGCTTG GGGGTCCTGC ACTTGGTGGG CAGCAGTCAG TATCCAACAA GCTCCTAGCC 
TGGAGTGGAG TCCTCGAGTG GCAGGAGAAA CCCAAGCCTG CATCTGTGGA TGCCAACACC 
AAGCTGACGA GGTCTCTGCC TTGCCAGGTC TATGTGAACC ATGGAGAAAA CCTGAAGACT 
GAGCAGTGGC CCCAGAAACT GATCATGCAG CTTATCCCTC AGCAGCTGCT GACCACCCTG 
GGTCCGCTGT TCCGGAACTC AAGAATGGTT CAGTTCCACT TCACCAACAA GGACCTGGAG 
TCCCTCAAAG GCCTCTACCG CATCATGGGC AATGGCTTTG CGGGCTGCGT GCATTTCCCG 
CACACGGCCC CATGCGAGGT GCGCGTGCTT ATGCTCCTGT ACTCGTCTAA GAAGAAGATC 
TTCATGGGCC TCATCCCCTA CGACCAGAGC GGCTTCGTCA ATGGCATCCG CCAGGTCATC 
ACCAACCACA AGCAGGTCCA GCAGCAGAAG CTGGAGCAAC AGCGCGGGCT ACAGCTCCGT 
GCACCACAGC CTCAGCCTCA GGGTGCCGTG GGAGCTTCTG CGGCTACAGG GCAGCCCCAG 
CCCCAAGGTG CTACCCAGGC CCCCACAGGC GCACCTCAAG GTCCTCCTGG AGCAGCCCCT 
GGCCCACCTC CTTCTGGACC CATCCTGCGG CCCCAGAACC CTGGGGCCAA CCCCCAGCTG 
CGGAGCCTTC TCCTTAACCC AGCACCGCCC CAGACTGGGG TGCCCCCGCC CCAGGCCTCC 
CTACACCACC TACAGCCTCC AGGGGCCCCT ACATTGCTGC CACCACATCA GAGCATGGGG 
CAGCCCCAGC TGGGGCCGCA GCTCCTGCAT CCTCCACCTG CCCAGTCATG GCCCACACAG 
CTTCCCCAGC GGGCGCCACT GCCAGTGGCC AAACGAAAGA GAGAAGGAGA GGGCCGCGTG 
TTTCGAGAAA AGTGGGAGCG AGACTATTTC TTTGTGGAAG TGAAGAGCAT GCCTACGTGC 
TTAATATGCA AAAAAAACGT GTCAGTGCTG AAAGAGTACA ACCTGAAGCG CCACTATGAG 
TCCCAGCACA GTAAGAGCTA CGATCAGTAC ACAGCGCAGA GCCGCGACAC ACTCCTGCAG 
GAACTGAAAA GGGCCCTGAG AGCCAGCGAG GCGCTGGAGA GCCAGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243067.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098927.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu89655
Clone ID Related Accession (Same CDS sequence)	XM_030243067.1
Accession Version	XM_030243067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGATGGGGGC ACAGCAGGCA 
CCTCCAGTCC TGGGCCCCAT TCTGGAGGAG CAAGCGAGGC CCCCGCAGAA TCTACTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GTGGCCAAAC GAAAGAGAGA AGGAGAGGGC 
CGCGTGTTTC GAGAAAAGTG GGAGCGAGAC TATTTCTTTG TGGAAGTGAA GAGCATGCCT 
ACGTGCTTAA TATGCAAAAA AAACGTGTCA GTGCTGAAAG AGTACAACCT GAAGCGCCAC 
TATGAGTCCC AGCACAGTAA GAGCTACGAT CAGTACACAG CGCAGAGCCG CGACACACTC 
CTGCAGGAAC TGAAAAGGGC CCTGAGAGCC AGCGAGGCGC TGGAGAGCCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098927.1
CDS	405..2501
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_030243067.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243067.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGATGGGGGC ACAGCAGGCA 
CCTCCAGTCC TGGGCCCCAT TCTGGAGGAG CAAGCGAGGC CCCCGCAGAA TCTACTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GTGGCCAAAC GAAAGAGAGA AGGAGAGGGC 
CGCGTGTTTC GAGAAAAGTG GGAGCGAGAC TATTTCTTTG TGGAAGTGAA GAGCATGCCT 
ACGTGCTTAA TATGCAAAAA AAACGTGTCA GTGCTGAAAG AGTACAACCT GAAGCGCCAC 
TATGAGTCCC AGCACAGTAA GAGCTACGAT CAGTACACAG CGCAGAGCCG CGACACACTC 
CTGCAGGAAC TGAAAAGGGC CCTGAGAGCC AGCGAGGCGC TGGAGAGCCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243068.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098928.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu89656
Clone ID Related Accession (Same CDS sequence)	XM_030243068.1
Accession Version	XM_030243068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGCTACA GCTCCGTGCA 
CCACAGCCTC AGCCTCAGGG TGCCGTGGGA GCTTCTGCGG CTACAGGGCA GCCCCAGCCC 
CAAGGTGCTA CCCAGGCCCC CACAGGCGCA CCTCAAGGTC CTCCTGGAGC AGCCCCTGGC 
CCACCTCCTT CTGGACCCAT CCTGCGGCCC CAGAACCCTG GGGCCAACCC CCAGCTGCGG 
AGCCTTCTCC TTAACCCAGC ACCGCCCCAG ACTGGGGTGC CCCCGCCCCA GGCCTCCCTA 
CACCACCTAC AGCCTCCAGG GGCCCCTACA TTGCTGCCAC CACATCAGAG CATGGGGCAG 
CCCCAGCTGG GGCCGCAGCT CCTGCATCCT CCACCTGCCC AGTCATGGCC CACACAGCTT 
CCCCAGCGGG CGCCACTGCC AGTGGCCAAA CGAAAGAGAG AAGGAGAGGG CCGCGTGTTT 
CGAGAAAAGT GGGAGCGAGA CTATTTCTTT GTGGAAGTGA AGAGCATGCC TACGTGCTTA 
ATATGCAAAA AAAACGTGTC AGTGCTGAAA GAGTACAACC TGAAGCGCCA CTATGAGTCC 
CAGCACAGTA AGAGCTACGA TCAGTACACA GCGCAGAGCC GCGACACACT CCTGCAGGAA 
CTGAAAAGGG CCCTGAGAGC CAGCGAGGCG CTGGAGAGCC AGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098928.1
CDS	404..2431
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_030243068.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243068.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGCTACA GCTCCGTGCA 
CCACAGCCTC AGCCTCAGGG TGCCGTGGGA GCTTCTGCGG CTACAGGGCA GCCCCAGCCC 
CAAGGTGCTA CCCAGGCCCC CACAGGCGCA CCTCAAGGTC CTCCTGGAGC AGCCCCTGGC 
CCACCTCCTT CTGGACCCAT CCTGCGGCCC CAGAACCCTG GGGCCAACCC CCAGCTGCGG 
AGCCTTCTCC TTAACCCAGC ACCGCCCCAG ACTGGGGTGC CCCCGCCCCA GGCCTCCCTA 
CACCACCTAC AGCCTCCAGG GGCCCCTACA TTGCTGCCAC CACATCAGAG CATGGGGCAG 
CCCCAGCTGG GGCCGCAGCT CCTGCATCCT CCACCTGCCC AGTCATGGCC CACACAGCTT 
CCCCAGCGGG CGCCACTGCC AGTGGCCAAA CGAAAGAGAG AAGGAGAGGG CCGCGTGTTT 
CGAGAAAAGT GGGAGCGAGA CTATTTCTTT GTGGAAGTGA AGAGCATGCC TACGTGCTTA 
ATATGCAAAA AAAACGTGTC AGTGCTGAAA GAGTACAACC TGAAGCGCCA CTATGAGTCC 
CAGCACAGTA AGAGCTACGA TCAGTACACA GCGCAGAGCC GCGACACACT CCTGCAGGAA 
CTGAAAAGGG CCCTGAGAGC CAGCGAGGCG CTGGAGAGCC AGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243069.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098929.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu89657
Clone ID Related Accession (Same CDS sequence)	XM_030243069.1
Accession Version	XM_030243069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGGTCAGA TGCTGCTGAG CGGGGGTCCC 
AGGGGCCCGG TCCCCCAGCC GGGCCTGCAG CCCAGCGTCA TGGAGGACGA CATCCTCATG 
GACCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098929.1
CDS	405..2336
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_030243069.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243069.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGATGGG GGCACAGCAG 
GCACCTCCAG TCCTGGGCCC CATTCTGGAG GAGCAAGCGA GGCCCCCGCA GAATCTACTA 
CAGCTCCGTG CACCACAGCC TCAGCCTCAG GGTGCCGTGG GAGCTTCTGC GGCTACAGGG 
CAGCCCCAGC CCCAAGGTGC TACCCAGGCC CCCACAGGCG CACCTCAAGG TCCTCCTGGA 
GCAGCCCCTG GCCCACCTCC TTCTGGACCC ATCCTGCGGC CCCAGAACCC TGGGGCCAAC 
CCCCAGCTGC GGAGCCTTCT CCTTAACCCA GCACCGCCCC AGACTGGGGT GCCCCCGCCC 
CAGGCCTCCC TACACCACCT ACAGCCTCCA GGGGCCCCTA CATTGCTGCC ACCACATCAG 
AGCATGGGGC AGCCCCAGCT GGGGCCGCAG CTCCTGCATC CTCCACCTGC CCAGTCATGG 
CCCACACAGC TTCCCCAGCG GGCGCCACTG CCAGGTCAGA TGCTGCTGAG CGGGGGTCCC 
AGGGGCCCGG TCCCCCAGCC GGGCCTGCAG CCCAGCGTCA TGGAGGACGA CATCCTCATG 
GACCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243071.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098931.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu89658
Clone ID Related Accession (Same CDS sequence)	XM_030243071.1 , XM_030243070.1
Accession Version	XM_030243071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGATGGGGGC ACAGCAGGCA 
CCTCCAGTCC TGGGCCCCAT TCTGGAGGAG CAAGCGAGGC CCCCGCAGAA TCTACTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GGTCAGATGC TGCTGAGCGG GGGTCCCAGG 
GGCCCGGTCC CCCAGCCGGG CCTGCAGCCC AGCGTCATGG AGGACGACAT CCTCATGGAC 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098931.1
CDS	406..2334
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_030243071.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243071.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGATGGGGGC ACAGCAGGCA 
CCTCCAGTCC TGGGCCCCAT TCTGGAGGAG CAAGCGAGGC CCCCGCAGAA TCTACTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GGTCAGATGC TGCTGAGCGG GGGTCCCAGG 
GGCCCGGTCC CCCAGCCGGG CCTGCAGCCC AGCGTCATGG AGGACGACAT CCTCATGGAC 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243070.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098930.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu89658
Clone ID Related Accession (Same CDS sequence)	XM_030243071.1 , XM_030243070.1
Accession Version	XM_030243070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGATGGGGGC ACAGCAGGCA 
CCTCCAGTCC TGGGCCCCAT TCTGGAGGAG CAAGCGAGGC CCCCGCAGAA TCTACTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GGTCAGATGC TGCTGAGCGG GGGTCCCAGG 
GGCCCGGTCC CCCAGCCGGG CCTGCAGCCC AGCGTCATGG AGGACGACAT CCTCATGGAC 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098930.1
CDS	404..2332
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_030243070.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243070.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGGCAGGCAC TGTAACCCCA GGTGGGGTCA ATGGTCCTTC AGCAGCCCAG 
CTTGGGGGTC CTGCACTTGG TGGGCAGCAG TCAGTATCCA ACAAGCTCCT AGCCTGGAGT 
GGAGTCCTCG AGTGGCAGGA GAAACCCAAG CCTGCATCTG TGGATGCCAA CACCAAGCTG 
ACGAGGTCTC TGCCTTGCCA GGTCTATGTG AACCATGGAG AAAACCTGAA GACTGAGCAG 
TGGCCCCAGA AACTGATCAT GCAGCTTATC CCTCAGCAGC TGCTGACCAC CCTGGGTCCG 
CTGTTCCGGA ACTCAAGAAT GGTTCAGTTC CACTTCACCA ACAAGGACCT GGAGTCCCTC 
AAAGGCCTCT ACCGCATCAT GGGCAATGGC TTTGCGGGCT GCGTGCATTT CCCGCACACG 
GCCCCATGCG AGGTGCGCGT GCTTATGCTC CTGTACTCGT CTAAGAAGAA GATCTTCATG 
GGCCTCATCC CCTACGACCA GAGCGGCTTC GTCAATGGCA TCCGCCAGGT CATCACCAAC 
CACAAGCAGG TCCAGCAGCA GAAGCTGGAG CAACAGCGCG GGATGGGGGC ACAGCAGGCA 
CCTCCAGTCC TGGGCCCCAT TCTGGAGGAG CAAGCGAGGC CCCCGCAGAA TCTACTACAG 
CTCCGTGCAC CACAGCCTCA GCCTCAGGGT GCCGTGGGAG CTTCTGCGGC TACAGGGCAG 
CCCCAGCCCC AAGGTGCTAC CCAGGCCCCC ACAGGCGCAC CTCAAGGTCC TCCTGGAGCA 
GCCCCTGGCC CACCTCCTTC TGGACCCATC CTGCGGCCCC AGAACCCTGG GGCCAACCCC 
CAGCTGCGGA GCCTTCTCCT TAACCCAGCA CCGCCCCAGA CTGGGGTGCC CCCGCCCCAG 
GCCTCCCTAC ACCACCTACA GCCTCCAGGG GCCCCTACAT TGCTGCCACC ACATCAGAGC 
ATGGGGCAGC CCCAGCTGGG GCCGCAGCTC CTGCATCCTC CACCTGCCCA GTCATGGCCC 
ACACAGCTTC CCCAGCGGGC GCCACTGCCA GGTCAGATGC TGCTGAGCGG GGGTCCCAGG 
GGCCCGGTCC CCCAGCCGGG CCTGCAGCCC AGCGTCATGG AGGACGACAT CCTCATGGAC 
CTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_030243072.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098932.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X13, mRNA.

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CloneID	OMu89659
Clone ID Related Accession (Same CDS sequence)	XM_030243072.1
Accession Version	XM_030243072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGCTACA GCTCCGTGCA 
CCACAGCCTC AGCCTCAGGG TGCCGTGGGA GCTTCTGCGG CTACAGGGCA GCCCCAGCCC 
CAAGGTGCTA CCCAGGCCCC CACAGGCGCA CCTCAAGGTC CTCCTGGAGC AGCCCCTGGC 
CCACCTCCTT CTGGACCCAT CCTGCGGCCC CAGAACCCTG GGGCCAACCC CCAGCTGCGG 
AGCCTTCTCC TTAACCCAGC ACCGCCCCAG ACTGGGGTGC CCCCGCCCCA GGCCTCCCTA 
CACCACCTAC AGCCTCCAGG GGCCCCTACA TTGCTGCCAC CACATCAGAG CATGGGGCAG 
CCCCAGCTGG GGCCGCAGCT CCTGCATCCT CCACCTGCCC AGTCATGGCC CACACAGCTT 
CCCCAGCGGG CGCCACTGCC AGGTCAGATG CTGCTGAGCG GGGGTCCCAG GGGCCCGGTC 
CCCCAGCCGG GCCTGCAGCC CAGCGTCATG GAGGACGACA TCCTCATGGA CCTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098932.1
CDS	405..2264
Translation	MGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_030243072.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243072.1

ORF Insert Sequence:

ATGGGCGGTG GTGGCGAGAG CTGTAGTCTC ATCGCTGAAG GTCTGAGCAC TGCCCTGCAG 
CTGTTCGATG ACTTTAAGAA GATGCGTGAA CAGATTGGCC AGACCCACCG AGTCTGTCTC 
CTCATCTGCA ACTCACCCCC GTACCTCCTG CCCGCCGTGG AGAGCACCAC CTACTCAGGC 
TGCACTACCG AGAGCCTGGT GCAGAAAATA GGAGAGCGAG GCATCCACTT CTCCATCGTT 
TCTCCGAGAA AGCTGCCAGC CCTCCGGCTT CTGTTTGAGA AGGCGGCTCC TCCTGCCCTA 
CTGGAGCCTT TGCAGCAACC AGCAGACGTC AGCCAGGACC CCAGGCACAT GGTGCTTGTG 
CGTGGGCTCG TGCTGCCTGT TGGTGGCAGC TCGACCTCAG GCTCTCTGCA GACCAAGCAA 
GCAGTCCCCT TGCCACCAGC TCCAGCTTCT GCTGCCACGC TGTCAGCAGC GCCCCCGCAG 
GCTCTGCCCC CTGTCCCACC ACAGTACCAG GTTCCTGGGA ACCTAAGTGC TGCTCAGGTG 
GCTGCGCAGA ATGCTGTGGA GGCTGCCAAG AGCCAGAAGG CCGGGCTGGG CCCACGCTTC 
TCACCCATCA ACCCTCTCCA GCAAGCTGCT CCAGGAGTGG GGCCCCCCTT CAGCCAGGCC 
CCAGCACCCC CATTGGCTCC TGTGCCCCCT GGAGCTCCAA AGCCACCTCC TGCCTCACAG 
CCTAGCCTAG TCTCCACCGT GGCCCCCGGA CCAGTCCTGG CAGCTCCTGC GCAGCCTGGG 
GCGCCGTCTC TGCAGGCAGG CACTGTAACC CCAGGTGGGG TCAATGGTCC TTCAGCAGCC 
CAGCTTGGGG GTCCTGCACT TGGTGGGCAG CAGTCAGTAT CCAACAAGCT CCTAGCCTGG 
AGTGGAGTCC TCGAGTGGCA GGAGAAACCC AAGCCTGCAT CTGTGGATGC CAACACCAAG 
CTGACGAGGT CTCTGCCTTG CCAGGTCTAT GTGAACCATG GAGAAAACCT GAAGACTGAG 
CAGTGGCCCC AGAAACTGAT CATGCAGCTT ATCCCTCAGC AGCTGCTGAC CACCCTGGGT 
CCGCTGTTCC GGAACTCAAG AATGGTTCAG TTCCACTTCA CCAACAAGGA CCTGGAGTCC 
CTCAAAGGCC TCTACCGCAT CATGGGCAAT GGCTTTGCGG GCTGCGTGCA TTTCCCGCAC 
ACGGCCCCAT GCGAGGTGCG CGTGCTTATG CTCCTGTACT CGTCTAAGAA GAAGATCTTC 
ATGGGCCTCA TCCCCTACGA CCAGAGCGGC TTCGTCAATG GCATCCGCCA GGTCATCACC 
AACCACAAGC AGGTCCAGCA GCAGAAGCTG GAGCAACAGC GCGGGCTACA GCTCCGTGCA 
CCACAGCCTC AGCCTCAGGG TGCCGTGGGA GCTTCTGCGG CTACAGGGCA GCCCCAGCCC 
CAAGGTGCTA CCCAGGCCCC CACAGGCGCA CCTCAAGGTC CTCCTGGAGC AGCCCCTGGC 
CCACCTCCTT CTGGACCCAT CCTGCGGCCC CAGAACCCTG GGGCCAACCC CCAGCTGCGG 
AGCCTTCTCC TTAACCCAGC ACCGCCCCAG ACTGGGGTGC CCCCGCCCCA GGCCTCCCTA 
CACCACCTAC AGCCTCCAGG GGCCCCTACA TTGCTGCCAC CACATCAGAG CATGGGGCAG 
CCCCAGCTGG GGCCGCAGCT CCTGCATCCT CCACCTGCCC AGTCATGGCC CACACAGCTT 
CCCCAGCGGG CGCCACTGCC AGGTCAGATG CTGCTGAGCG GGGGTCCCAG GGGCCCGGTC 
CCCCAGCCGG GCCTGCAGCC CAGCGTCATG GAGGACGACA TCCTCATGGA CCTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_006541263.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541326.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X4, mRNA.

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CloneID	OMu39090
Clone ID Related Accession (Same CDS sequence)	XM_006541263.1 , XM_006541263.2
Accession Version	XM_006541263.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541326.1
CDS	124..2541
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_006541263.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541263.1

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG GTCAGATGCT GCTGAGCGGG 
GGTCCCAGGG GCCCGGTCCC CCAGCCGGGC CTGCAGCCCA GCGTCATGGA GGACGACATC 
CTCATGGACC TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_006541264.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541327.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu39091
Clone ID Related Accession (Same CDS sequence)	XM_006541264.2 , XM_006541264.1
Accession Version	XM_006541264.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGGC AGGCACTGTA ACCCCAGGTG GGGTCAATGG TCCTTCAGCA 
GCCCAGCTTG GGGGTCCTGC ACTTGGTGGG CAGCAGTCAG TATCCAACAA GCTCCTAGCC 
TGGAGTGGAG TCCTCGAGTG GCAGGAGAAA CCCAAGCCTG CATCTGTGGA TGCCAACACC 
AAGCTGACGA GGTCTCTGCC TTGCCAGGTC TATGTGAACC ATGGAGAAAA CCTGAAGACT 
GAGCAGTGGC CCCAGAAACT GATCATGCAG CTTATCCCTC AGCAGCTGCT GACCACCCTG 
GGTCCGCTGT TCCGGAACTC AAGAATGGTT CAGTTCCACT TCACCAACAA GGACCTGGAG 
TCCCTCAAAG GCCTCTACCG CATCATGGGC AATGGCTTTG CGGGCTGCGT GCATTTCCCG 
CACACGGCCC CATGCGAGGT GCGCGTGCTT ATGCTCCTGT ACTCGTCTAA GAAGAAGATC 
TTCATGGGCC TCATCCCCTA CGACCAGAGC GGCTTCGTCA ATGGCATCCG CCAGGTCATC 
ACCAACCACA AGCAGGTCCA GCAGCAGAAG CTGGAGCAAC AGCGCGGGCT ACAGCTCCGT 
GCACCACAGC CTCAGCCTCA GGGTGCCGTG GGAGCTTCTG CGGCTACAGG GCAGCCCCAG 
CCCCAAGGTG CTACCCAGGC CCCCACAGGC GCACCTCAAG GTCCTCCTGG AGCAGCCCCT 
GGCCCACCTC CTTCTGGACC CATCCTGCGG CCCCAGAACC CTGGGGCCAA CCCCCAGCTG 
CGGAGCCTTC TCCTTAACCC AGCACCGCCC CAGACTGGGG TGCCCCCGCC CCAGGCCTCC 
CTACACCACC TACAGCCTCC AGGGGCCCCT ACATTGCTGC CACCACATCA GAGCATGGGG 
CAGCCCCAGC TGGGGCCGCA GCTCCTGCAT CCTCCACCTG CCCAGTCATG GCCCACACAG 
CTTCCCCAGC GGGCGCCACT GCCAGGTCAG ATGCTGCTGA GCGGGGGTCC CAGGGGCCCG 
GTCCCCCAGC CGGGCCTGCA GCCCAGCGTC ATGGAGGACG ACATCCTCAT GGACCTCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541327.1
CDS	124..2466
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_006541264.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541264.1

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGGC AGGCACTGTA ACCCCAGGTG GGGTCAATGG TCCTTCAGCA 
GCCCAGCTTG GGGGTCCTGC ACTTGGTGGG CAGCAGTCAG TATCCAACAA GCTCCTAGCC 
TGGAGTGGAG TCCTCGAGTG GCAGGAGAAA CCCAAGCCTG CATCTGTGGA TGCCAACACC 
AAGCTGACGA GGTCTCTGCC TTGCCAGGTC TATGTGAACC ATGGAGAAAA CCTGAAGACT 
GAGCAGTGGC CCCAGAAACT GATCATGCAG CTTATCCCTC AGCAGCTGCT GACCACCCTG 
GGTCCGCTGT TCCGGAACTC AAGAATGGTT CAGTTCCACT TCACCAACAA GGACCTGGAG 
TCCCTCAAAG GCCTCTACCG CATCATGGGC AATGGCTTTG CGGGCTGCGT GCATTTCCCG 
CACACGGCCC CATGCGAGGT GCGCGTGCTT ATGCTCCTGT ACTCGTCTAA GAAGAAGATC 
TTCATGGGCC TCATCCCCTA CGACCAGAGC GGCTTCGTCA ATGGCATCCG CCAGGTCATC 
ACCAACCACA AGCAGGTCCA GCAGCAGAAG CTGGAGCAAC AGCGCGGGCT ACAGCTCCGT 
GCACCACAGC CTCAGCCTCA GGGTGCCGTG GGAGCTTCTG CGGCTACAGG GCAGCCCCAG 
CCCCAAGGTG CTACCCAGGC CCCCACAGGC GCACCTCAAG GTCCTCCTGG AGCAGCCCCT 
GGCCCACCTC CTTCTGGACC CATCCTGCGG CCCCAGAACC CTGGGGCCAA CCCCCAGCTG 
CGGAGCCTTC TCCTTAACCC AGCACCGCCC CAGACTGGGG TGCCCCCGCC CCAGGCCTCC 
CTACACCACC TACAGCCTCC AGGGGCCCCT ACATTGCTGC CACCACATCA GAGCATGGGG 
CAGCCCCAGC TGGGGCCGCA GCTCCTGCAT CCTCCACCTG CCCAGTCATG GCCCACACAG 
CTTCCCCAGC GGGCGCCACT GCCAGGTCAG ATGCTGCTGA GCGGGGGTCC CAGGGGCCCG 
GTCCCCCAGC CGGGCCTGCA GCCCAGCGTC ATGGAGGACG ACATCCTCAT GGACCTCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_017312366.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167855.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82246
Clone ID Related Accession (Same CDS sequence)	XM_017312366.1 , XM_017312366.2
Accession Version	XM_017312366.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG TGGCCAAACG AAAGAGAGAA 
GGAGAGGGCC GCGTGTTTCG AGAAAAGTGG GAGCGAGACT ATTTCTTTGT GGAAGTGAAG 
AGCATGCCTA CGTGCTTAAT ATGCAAAAAA AACGTGTCAG TGCTGAAAGA GTACAACCTG 
AAGCGCCACT ATGAGTCCCA GCACAGTAAG AGCTACGATC AGTACACAGC GCAGAGCCGC 
GACACACTCC TGCAGGAACT GAAAAGGGCC CTGAGAGCCA GCGAGGCGCT GGAGAGCCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167855.1
CDS	124..2709
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALESQE

Target ORF information:

RefSeq Version	XM_017312366.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312366.1

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GATGGGGGCA 
CAGCAGGCAC CTCCAGTCCT GGGCCCCATT CTGGAGGAGC AAGCGAGGCC CCCGCAGAAT 
CTACTACAGC TCCGTGCACC ACAGCCTCAG CCTCAGGGTG CCGTGGGAGC TTCTGCGGCT 
ACAGGGCAGC CCCAGCCCCA AGGTGCTACC CAGGCCCCCA CAGGCGCACC TCAAGGTCCT 
CCTGGAGCAG CCCCTGGCCC ACCTCCTTCT GGACCCATCC TGCGGCCCCA GAACCCTGGG 
GCCAACCCCC AGCTGCGGAG CCTTCTCCTT AACCCAGCAC CGCCCCAGAC TGGGGTGCCC 
CCGCCCCAGG CCTCCCTACA CCACCTACAG CCTCCAGGGG CCCCTACATT GCTGCCACCA 
CATCAGAGCA TGGGGCAGCC CCAGCTGGGG CCGCAGCTCC TGCATCCTCC ACCTGCCCAG 
TCATGGCCCA CACAGCTTCC CCAGCGGGCG CCACTGCCAG TGGCCAAACG AAAGAGAGAA 
GGAGAGGGCC GCGTGTTTCG AGAAAAGTGG GAGCGAGACT ATTTCTTTGT GGAAGTGAAG 
AGCATGCCTA CGTGCTTAAT ATGCAAAAAA AACGTGTCAG TGCTGAAAGA GTACAACCTG 
AAGCGCCACT ATGAGTCCCA GCACAGTAAG AGCTACGATC AGTACACAGC GCAGAGCCGC 
GACACACTCC TGCAGGAACT GAAAAGGGCC CTGAGAGCCA GCGAGGCGCT GGAGAGCCAG 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med25 ( XM_017312367.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167856.1 Mus musculus mediator complex subunit 25 (Med25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu82247
Clone ID Related Accession (Same CDS sequence)	XM_017312367.1 , XM_017312367.2
Accession Version	XM_017312367.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GCTACAGCTC 
CGTGCACCAC AGCCTCAGCC TCAGGGTGCC GTGGGAGCTT CTGCGGCTAC AGGGCAGCCC 
CAGCCCCAAG GTGCTACCCA GGCCCCCACA GGCGCACCTC AAGGTCCTCC TGGAGCAGCC 
CCTGGCCCAC CTCCTTCTGG ACCCATCCTG CGGCCCCAGA ACCCTGGGGC CAACCCCCAG 
CTGCGGAGCC TTCTCCTTAA CCCAGCACCG CCCCAGACTG GGGTGCCCCC GCCCCAGGCC 
TCCCTACACC ACCTACAGCC TCCAGGGGCC CCTACATTGC TGCCACCACA TCAGAGCATG 
GGGCAGCCCC AGCTGGGGCC GCAGCTCCTG CATCCTCCAC CTGCCCAGTC ATGGCCCACA 
CAGCTTCCCC AGCGGGCGCC ACTGCCAGGT CAGATGCTGC TGAGCGGGGG TCCCAGGGGC 
CCGGTCCCCC AGCCGGGCCT GCAGCCCAGC GTCATGGAGG ACGACATCCT CATGGACCTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167856.1
CDS	124..2469
Translation	MPQGRDKTTARKMPRTNMAVSASAEAARRPAGLRTPRKRTSVRPGTATASAALASAARGMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLQAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI

Target ORF information:

RefSeq Version	XM_017312367.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 25 (Med25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312367.1

ORF Insert Sequence:

ATGCCCCAGG GTAGAGATAA AACCACGGCT CGCAAAATGC CCCGCACAAA CATGGCCGTA 
TCAGCATCAG CAGAAGCTGC CAGACGTCCG GCGGGGCTGC GCACACCGCG CAAGCGCACT 
TCTGTTCGTC CCGGGACGGC GACGGCGTCA GCGGCGCTGG CGTCGGCGGC GCGGGGCATG 
GTCCCCGGAT CCGAAGGCCC GGCGCGGGCC GGGGGCCTGG TCGCCGATGT GGTGTTTGTG 
ATCGAGGGGA CAGCAAACCT GGGGCCGTAC TTCGAGGAGC TCCGCAAGCA CTACCTGCTG 
CCTGCCATAG AGTACTTCAA CGGGGGACCC CCTGCAGAGA CGGACTTTGG GGGAGACTAT 
GGTGGAACCC AGTACAGCCT TGTGGTGTTC AACACCGTGG ACTGCGCTCC AGAGTCCTAT 
GTACAATGTC ACGCACCTAC CAGCAGTGCC TATGAGTTTG TCACCTGGCT CGATGGCATC 
AAGTTCATGG GCGGTGGTGG CGAGAGCTGT AGTCTCATCG CTGAAGGTCT GAGCACTGCC 
CTGCAGCTGT TCGATGACTT TAAGAAGATG CGTGAACAGA TTGGCCAGAC CCACCGAGTC 
TGTCTCCTCA TCTGCAACTC ACCCCCGTAC CTCCTGCCCG CCGTGGAGAG CACCACCTAC 
TCAGGCTGCA CTACCGAGAG CCTGGTGCAG AAAATAGGAG AGCGAGGCAT CCACTTCTCC 
ATCGTTTCTC CGAGAAAGCT GCCAGCCCTC CGGCTTCTGT TTGAGAAGGC GGCTCCTCCT 
GCCCTACTGG AGCCTTTGCA GCAACCAGCA GACGTCAGCC AGGACCCCAG GCACATGGTG 
CTTGTGCGTG GGCTCGTGCT GCCTGTTGGT GGCAGCTCGA CCTCAGGCTC TCTGCAGACC 
AAGCAAGCAG TCCCCTTGCC ACCAGCTCCA GCTTCTGCTG CCACGCTGTC AGCAGCGCCC 
CCGCAGGCTC TGCCCCCTGT CCCACCACAG TACCAGGTTC CTGGGAACCT AAGTGCTGCT 
CAGGTGGCTG CGCAGAATGC TGTGGAGGCT GCCAAGAGCC AGAAGGCCGG GCTGGGCCCA 
CGCTTCTCAC CCATCAACCC TCTCCAGCAA GCTGCTCCAG GAGTGGGGCC CCCCTTCAGC 
CAGGCCCCAG CACCCCCATT GGCTCCTGTG CCCCCTGGAG CTCCAAAGCC ACCTCCTGCC 
TCACAGCCTA GCCTAGTCTC CACCGTGGCC CCCGGACCAG TCCTGGCAGC TCCTGCGCAG 
CCTGGGGCGC CGTCTCTGCA GGCAGGCACT GTAACCCCAG GTGGGGTCAA TGGTCCTTCA 
GCAGCCCAGC TTGGGGGTCC TGCACTTGGT GGGCAGCAGT CAGTATCCAA CAAGCTCCTA 
GCCTGGAGTG GAGTCCTCGA GTGGCAGGAG AAACCCAAGC CTGCATCTGT GGATGCCAAC 
ACCAAGCTGA CGAGGTCTCT GCCTTGCCAG GTCTATGTGA ACCATGGAGA AAACCTGAAG 
ACTGAGCAGT GGCCCCAGAA ACTGATCATG CAGCTTATCC CTCAGCAGCT GCTGACCACC 
CTGGGTCCGC TGTTCCGGAA CTCAAGAATG GTTCAGTTCC ACTTCACCAA CAAGGACCTG 
GAGTCCCTCA AAGGCCTCTA CCGCATCATG GGCAATGGCT TTGCGGGCTG CGTGCATTTC 
CCGCACACGG CCCCATGCGA GGTGCGCGTG CTTATGCTCC TGTACTCGTC TAAGAAGAAG 
ATCTTCATGG GCCTCATCCC CTACGACCAG AGCGGCTTCG TCAATGGCAT CCGCCAGGTC 
ATCACCAACC ACAAGCAGGT CCAGCAGCAG AAGCTGGAGC AACAGCGCGG GCTACAGCTC 
CGTGCACCAC AGCCTCAGCC TCAGGGTGCC GTGGGAGCTT CTGCGGCTAC AGGGCAGCCC 
CAGCCCCAAG GTGCTACCCA GGCCCCCACA GGCGCACCTC AAGGTCCTCC TGGAGCAGCC 
CCTGGCCCAC CTCCTTCTGG ACCCATCCTG CGGCCCCAGA ACCCTGGGGC CAACCCCCAG 
CTGCGGAGCC TTCTCCTTAA CCCAGCACCG CCCCAGACTG GGGTGCCCCC GCCCCAGGCC 
TCCCTACACC ACCTACAGCC TCCAGGGGCC CCTACATTGC TGCCACCACA TCAGAGCATG 
GGGCAGCCCC AGCTGGGGCC GCAGCTCCTG CATCCTCCAC CTGCCCAGTC ATGGCCCACA 
CAGCTTCCCC AGCGGGCGCC ACTGCCAGGT CAGATGCTGC TGAGCGGGGG TCCCAGGGGC 
CCGGTCCCCC AGCCGGGCCT GCAGCCCAGC GTCATGGAGG ACGACATCCT CATGGACCTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

MED25 is a mediator component of HNF4?-driven transcription leading to insulin secretion in pancreatic beta-cells.
PloS one7(8)e44007(2012)
Han EH,Rha GB,Chi YI

Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25.
Nature communications2251(2011)
Nakamura Y,Yamamoto K,He X,Otsuki B,Kim Y,Murao H,Soeda T,Tsumaki N,Deng JM,Zhang Z,Behringer RR,Crombrugghe Bd,Postlethwait JH,Warman ML,Nakamura T,Akiyama H

Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.
Neurogenetics10(4)275-87(2009 Oct)
Leal A,Huehne K,Bauer F,Sticht H,Berger P,Suter U,Morera B,Del Valle G,Lupski JR,Ekici A,Pasutto F,Endele S,Barrantes R,Berghoff C,Berghoff M,Neund?rfer B,Heuss D,Dorn T,Young P,Santolin L,Uhlmann T,Meisterernst M,Sereda MW,Sereda M,Stassart RM,Meyer zu Horste G,Nave KA,Reis A,Rautenstrauss B

MED25 is distinct from TRAP220/MED1 in cooperating with CBP for retinoid receptor activation.
The EMBO journal26(15)3545-57(2007 Aug)
Lee HK,Park UH,Kim EJ,Um SJ

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Med25 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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