Rhoj cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhoj
Entrez Gene ID	80837
Full Name	ras homolog family member J
Synonyms	1110005O19Rik,AW210585,Arhj,TC10L,TCL
General protein information	Preferred Names ras homolog family member J Names rho-related GTP-binding protein RhoJ TC10-like Rho GTPase ras homolog gene family, member J tc10-like GTP-binding protein TCL
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_023275.2	NP_075764.1	rho-related GTP-binding protein RhoJ precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RHOJ	NP_001264477.1
Pan troglodytes (chimpanzee)	RHOJ	XP_509996.2
Bos taurus (cattle)	RHOJ	NP_001076967.1
Rattus norvegicus (Norway rat)	Rhoj	NP_001008321.1
Danio rerio (zebrafish)	rhoj	NP_001038266.1
Xenopus tropicalis (tropical clawed frog)	rhoj	XP_002939520.1
	RHOJ	XP_001100323.1
Mus musculus (house mouse)	Rhoj	NP_075764.1
Homo sapiens (human)	RHOJ	NP_065714.1
Canis lupus familiaris (dog)	RHOJ	XP_854125.2

Gene Ontology
Bibliography

Related articles in PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

The RhoJ-BAD signaling network: An Achilles' heel for BRAF mutant melanomas.
Ruiz R, Jahid S, Harris M, Marzese DM, Espitia F, Vasudeva P, Chen CF, de Feraudy S, Wu J, Gillen DL, Krasieva TB, Tromberg BJ, Pavan WJ, Hoon DS, Ganesan AK
PLoS genetics13(7)e1006913(2017 Jul)

The Genetic Basis of Baculum Size and Shape Variation in Mice.
Schultz NG, Ingels J, Hillhouse A, Wardwell K, Chang PL, Cheverud JM, Lutz C, Lu L, Williams RW, Dean MD
G3 (Bethesda, Md.)6(5)1141-51(2016 05)

RhoJ interacts with the GIT-PIX complex and regulates focal adhesion disassembly.
Wilson E, Leszczynska K, Poulter NS, Edelmann F, Salisbury VA, Noy PJ, Bacon A, Rappoport JZ, Heath JK, Bicknell R, Heath VL
Journal of cell science127(Pt 14)3039-51(2014 Jul)

Vascular RhoJ is an effective and selective target for tumor angiogenesis and vascular disruption.
Kim C, Yang H, Fukushima Y, Saw PE, Lee J, Park JS, Park I, Jung J, Kataoka H, Lee D, Heo WD, Kim I, Jon S, Adams RH, Nishikawa S, Uemura A, Koh GY
Cancer cell25(1)102-17(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RhoJ-knockout mice showed reduced tumour growth and diminished tumour vessel density, identifying a role for RhoJ in mediating tumour angiogenesis. Studies give insight into the molecular function of RhoJ in regulating cell motility.
Title: RhoJ interacts with the GIT-PIX complex and regulates focal adhesion disassembly.

These results identify RhoJ blockade as a selective and effective therapeutic strategy for targeting tumor vasculature with minimal side effects.
Title: Vascular RhoJ is an effective and selective target for tumor angiogenesis and vascular disruption.

RhoJ is endothelial-expressed in vivo, activated by vascular endothelial growth factor, localizes to focal adhesions, regulates endothelial cell migration and tube formation, and modulates actomyosin contractility and focal adhesion numbers.
Title: RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers.

The results strongly suggest that TCL/TC10betaL regulates adipocyte differentiation by controlling mitotic clonal expansion and this regulatory effect is closely linked to C/EBPbeta and C/EBPdelta expression.
Title: TC10-like/TC10betaLong regulates adipogenesis by controlling mitotic clonal expansion.

Insulin induces phosphatidylinositol 3-phosphate formation through TC10 activation.
Title: Insulin induces phosphatidylinositol-3-phosphate formation through TC10 activation.

The following Rhoj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhoj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16318	NM_023275.2 Latest version!	Mus musculus ras homolog family member J (Rhoj), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhoj ( NM_023275.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075764.1 Mus musculus ras homolog family member J (Rhoj), mRNA.

Price & Availability↑

CloneID	OMu16318
Clone ID Related Accession (Same CDS sequence)	NM_023275.2
Accession Version	NM_023275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoJ precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY004626.1 and AK003482.1. On Jul 19, 2005 this sequence version replaced NM_023275.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159385.1, AK157518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTGCA GAGAGAGGAC CGACAGCAGC TGCGGCTGCA ATGGACATGA GGAGAACAGG 
ATCCTGAAGT GCGTGGTGGT CGGGGACGGC GCGGTGGGGA AGACCTGCTT GCTGATGAGC 
TACGCCAACG ACGCCTTCCC AGAGGAATAC GTGCCCACTG TGTTTGACCA CTACGCAGTT 
ACCGTGACGG TGGGAGGCAA GCAGCACTTG CTTGGACTAT ACGACACTGC AGGACAGGAG 
GATTACAACC AGCTGCGGCC ACTCTCTTAC CCCAACACAG ATGTGTTTCT CATCTGCTTC 
TCTGTCGTAA ACCCAGCCTC TTACCACAAC GTTCAGGAGG AGTGGGTCCC GGAACTGAAG 
GACTGCATGC CTCATGTGCC TTATGTGCTC ATCGGAACCC AGATTGATCT CCGAGATGAC 
CCCAAAACCT TGGCGCGTCT GCTGTACATG AAGGAGAAGC CCCTCACCTA TGAGCACGGC 
GTGAAGCTGG CGAAAGCGAT TGGAGCGCAG TGCTACTTGG AATGTTCTGC CCTGACCCAG 
AAGGGTCTCA AAGCAGTTTT TGATGAAGCC ATCCTCACCA TTTTCCACCC CAAGAAAAAA 
AAGAAAGGCT GCTTAGGGTG TCACGGCTGC TGTGCAATTA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075764.1
CDS	512..1156
Translation	MSCRERTDSSCGCNGHEENRILKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKGCLGCHGCCAII

Target ORF information:

RefSeq Version	NM_023275.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member J (Rhoj), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023275.2

ORF Insert Sequence:

ATGAGCTGCA GAGAGAGGAC CGACAGCAGC TGCGGCTGCA ATGGACATGA GGAGAACAGG 
ATCCTGAAGT GCGTGGTGGT CGGGGACGGC GCGGTGGGGA AGACCTGCTT GCTGATGAGC 
TACGCCAACG ACGCCTTCCC AGAGGAATAC GTGCCCACTG TGTTTGACCA CTACGCAGTT 
ACCGTGACGG TGGGAGGCAA GCAGCACTTG CTTGGACTAT ACGACACTGC AGGACAGGAG 
GATTACAACC AGCTGCGGCC ACTCTCTTAC CCCAACACAG ATGTGTTTCT CATCTGCTTC 
TCTGTCGTAA ACCCAGCCTC TTACCACAAC GTTCAGGAGG AGTGGGTCCC GGAACTGAAG 
GACTGCATGC CTCATGTGCC TTATGTGCTC ATCGGAACCC AGATTGATCT CCGAGATGAC 
CCCAAAACCT TGGCGCGTCT GCTGTACATG AAGGAGAAGC CCCTCACCTA TGAGCACGGC 
GTGAAGCTGG CGAAAGCGAT TGGAGCGCAG TGCTACTTGG AATGTTCTGC CCTGACCCAG 
AAGGGTCTCA AAGCAGTTTT TGATGAAGCC ATCCTCACCA TTTTCCACCC CAAGAAAAAA 
AAGAAAGGCT GCTTAGGGTG TCACGGCTGC TGTGCAATTA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

The RhoJ-BAD signaling network: An Achilles' heel for BRAF mutant melanomas.
PLoS genetics13(7)e1006913(2017 Jul)
Ruiz R,Jahid S,Harris M,Marzese DM,Espitia F,Vasudeva P,Chen CF,de Feraudy S,Wu J,Gillen DL,Krasieva TB,Tromberg BJ,Pavan WJ,Hoon DS,Ganesan AK

The Genetic Basis of Baculum Size and Shape Variation in Mice.
G3 (Bethesda, Md.)6(5)1141-51(2016 05)
Schultz NG,Ingels J,Hillhouse A,Wardwell K,Chang PL,Cheverud JM,Lutz C,Lu L,Williams RW,Dean MD

RhoJ interacts with the GIT-PIX complex and regulates focal adhesion disassembly.
Journal of cell science127(Pt 14)3039-51(2014 Jul)
Wilson E,Leszczynska K,Poulter NS,Edelmann F,Salisbury VA,Noy PJ,Bacon A,Rappoport JZ,Heath JK,Bicknell R,Heath VL

Vascular RhoJ is an effective and selective target for tumor angiogenesis and vascular disruption.
Cancer cell25(1)102-17(2014 Jan)
Kim C,Yang H,Fukushima Y,Saw PE,Lee J,Park JS,Park I,Jung J,Kataoka H,Lee D,Heo WD,Kim I,Jon S,Adams RH,Nishikawa S,Uemura A,Koh GY

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Rhoj cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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